CN103478493B - Formula of artificial feed for dichocrocis punctiferalis, and manufacturing method of artificial feed - Google Patents
Formula of artificial feed for dichocrocis punctiferalis, and manufacturing method of artificial feed Download PDFInfo
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Abstract
The invention discloses a formula of an artificial feed for dichocrocis punctiferalis, and a manufacturing method of the artificial feed and belongs to the field of artificial feeds for insects. The feed comprises Chinese chestnut powder, maize meal, peach powder, soybean flour, beer yeast powder, multivita-glucose, cholesterol, casein, ascorbic acid, erythromycin, agar powder, methylparaben, methyl parahydroxybenzoats and water. The invention further provides a manufacturing method of the artificial feed for dichocrocis punctiferalis. According to the artificial feed, the Chinese chestnut powder, the maize meal, the soybean flour, the beer yeast powder and the like are added and are in coordination with a plurality of trace elements, so that on the premise of improving the survival rate, the fecundity and the like of dichocrocis punctiferalis, the raising cost is lowered effectively, and the problem that when the conventional artificial feed is used for feeding dichocrocis punctiferalis, larvae refuse feed, the survival rate is low, and the development is not uniform can be solved.
Description
Technical field
The present invention relates to insect feedstuff development field, particularly relate to a kind of man-made feeds formula and preparation method for artificial feeding dichocrocis punctiferalis.
Background technology
Raising insect with man-made feeds is one of basic fundamental entomologizing, can not be subject to host and season limit to breed the object insect of certain kind, for research (Wang Yannian etc., 1984 of the aspects such as insect biology, physiology and integrated pest control measure formulation; 1990).
(Guen é e) is under the jurisdiction of Lepidoptera, Pyralidae to dichocrocis punctiferalis Conogethes punctiferalis, a kind of important omnivorousness and borer pest, it is reported, its host relates to more than 100 kind of fruit tree, forest and the crops such as Chinese chestnut, peach, Lee (deer gold autumn etc., 2010).At present, along with dichocrocis punctiferalis is ecological and the deepening continuously of Study of Prevention Technology, particularly carrying out in a deep going way of the relevant research work such as biological control and resistance to the action of a drug mensuration, needs supply in a large number to try worm sources.Therefore, artificial feeding dichocrocis punctiferalis under laboratory condition, highlights its significance gradually for scientific experiment provides standard examination worm, and there is a correlative study report in China about dichocrocis punctiferalis artificial feeding is rarely seen.The artificial feeding technology of dichocrocis punctiferalis was once had to report abroad, but because some composition in feed is composite parts, do not list respectively, some composition China yet there are no Related product, is difficult to promotion and application at present in China.As: the antiseptic (Antimicrobial agent mixture) of mentioning in Honda etc. (1979) feed formula is unknown composite parts, and its dusty yeast used (Meal powder for mouse) is external product.In addition, while raising with existing man-made feeds, when not only survival rate of larvae is raised compared with natural host plant, significantly reduce, and polus animalium irregular (Honda et al., 1979; Utsumi et al., 1990).
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of dichocrocis punctiferalis man-made feeds formula and preparation method, significantly reduce when thereby not only survival rate of larvae is raised compared with natural host plant while solving existing man-made feeds raising dichocrocis punctiferalis, and the irregular problem of polus animalium.
For solving the problems of the technologies described above, the invention provides a kind of dichocrocis punctiferalis man-made feeds formula, this feed formula is made up of rice-chestnut powder, corn flour, peach powder, soy meal, beer yeast powder, multivita-glucose, casein, cholesterol, ascorbic acid, erythromycin, agar powder, methyl hydroxybenzoate, methyl p-hydroxybenzoate and water.
The present invention also provides a kind of preparation method of dichocrocis punctiferalis man-made feeds, adopts dichocrocis punctiferalis man-made feeds formula of the present invention, comprises the following steps:
The first step: according to recipe ingredient and consumption, weigh corresponding rice-chestnut powder, corn flour, peach powder, soy meal and beer yeast powder, after mixing, put into pot steam 30 minutes form mixture stand-by;
Second step: water intaking is put into pot and heated after adding the continuous stirring of agar powder to make it fully to dissolve, adds multivita-glucose simultaneously, stirs and makes it to be dissolved in agar solution, and wherein, the consumption of water is 20 times of agar powder consumption by weight;
The 3rd step, when the agar solution of making is cooled to 60 DEG C, adds methyl hydroxybenzoate, methyl p-hydroxybenzoate, erythromycin, ascorbic acid and cholesterol until second step, and each component mixes makes homogenate;
The 4th step: the mixture furnishing pasty state first step being made with remaining water, pour in the homogenate that the 3rd step makes, limit bevelling is poured in container after being stirred to evenly, to be cooled to gel state, open container and be placed on 4 DEG C of refrigerations after 24 hours, make the man-made feeds of dichocrocis punctiferalis.
Beneficial effect of the present invention is: the host plant that these man-made feeds are eaten taking dichocrocis punctiferalis happiness is as main component, by adding carbohydrate, protein, various trace elements and anticorrisive agent etc., improving under the prerequisite of the indexs such as dichocrocis punctiferalis survival rate and reproductive capacity, effectively reduce feeding cost, problem while having avoided existing man-made feeds to raise for dichocrocis punctiferalis the problem includes: larva food refusal, survival rate low, grow the problems such as irregular.
Detailed description of the invention
Technical scheme in the embodiment of the present invention is carried out to clear, complete description below, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiment.Based on embodiments of the invention, those of ordinary skill in the art, not making the every other embodiment obtaining under creative work prerequisite, belong to protection scope of the present invention.
The embodiment of the present invention provides a kind of dichocrocis punctiferalis man-made feeds formula, be a kind of feed formula for artificial feeding dichocrocis punctiferalis, this feed formula is made up of rice-chestnut powder, corn flour, peach powder, soy meal, beer yeast powder, multivita-glucose, cholesterol, casein, ascorbic acid, erythromycin, agar powder, methyl hydroxybenzoate, methyl p-hydroxybenzoate and water.
Preferably, in above-mentioned man-made feeds, the consumption of each raw material by mass parts is:
Rice-chestnut powder 40, corn flour 90, peach powder 10, soy meal 30, beer yeast powder 30, multivita-glucose 10, casein 10, cholesterol 0.1, ascorbic acid 2, erythromycin 3, agar powder 10, methyl hydroxybenzoate 1, methyl p-hydroxybenzoate 2 and water 700.
Preferably, the hydromining distilled water in above-mentioned man-made feeds.
The preparation method of above-mentioned man-made feeds is: measure each raw material by the use of above-mentioned formula, make by following steps:
The first step: according to recipe ingredient and consumption, weigh corresponding rice-chestnut powder, corn flour, peach powder, soy meal and beer yeast powder, after mixing, put into pot steam 30 minutes form mixture stand-by;
Second step: water intaking is put into pot and heated, after adding the continuous stirring of agar powder to make it fully to dissolve simultaneously, add multivita-glucose, stirring makes it to be dissolved in agar solution, wherein, the consumption of water is 20 times of agar powder consumption, if total water amount is 700ml, in this step, adding 200ml, is the equal of 20 times of 10 grams of agar powder consumptions by weight;
The 3rd step, when the agar solution of making is cooled to 60 DEG C, adds methyl hydroxybenzoate, methyl p-hydroxybenzoate, erythromycin, ascorbic acid and cholesterol until second step, and each component mixes makes homogenate; In the 3rd step, each component mixes process temperature and is controlled at below 60 DEG C;
The 4th step: the mixture furnishing pasty state first step being made with remaining water, pour in the homogenate that the 3rd step makes, limit bevelling is poured in container (as adopted crisper) after being stirred to evenly, to be cooled to gel state, open container and be placed on 4 DEG C of refrigerations after 24 hours (available refrigerator cold-storage), make the man-made feeds of dichocrocis punctiferalis.
It is stored refrigerated at 4 DEG C that said method makes the man-made feeds of dichocrocis punctiferalis.
Below in conjunction with concrete analysis process, man-made feeds and preparation method to above-described embodiment are described further.
The invention provides the man-made feeds formula of a kind of dichocrocis punctiferalis, for laboratory artificial feeding and the amount reproduction of this worm.Described dichocrocis punctiferalis man-made feeds comprise following component: each ratio of components is in table 1, formula I in table 1 is man-made feeds formula of the present invention, other Three feed formula, for the present invention's formula comparative formula of difference to some extent, in order to compare with the raising result of formula I.
Table 1 dichocrocis punctiferalis larva artificial feed formula
Above-mentioned dichocrocis punctiferalis man-made feeds preparation method, can prepare dichocrocis punctiferalis man-made feeds, and for its indoor population breed in all seasons, the biology, physiology, toxicology and the ecological study that can be this worm provide a large amount of examination worms.The method adopts above-mentioned raw materials and consumption, comprises the following steps:
The first step: according to recipe ingredient and consumption, weigh corresponding rice-chestnut powder, corn flour, peach powder, apple powder, soy meal, beer yeast powder and chestnut shell powder (for different formulas, if do not contain a certain raw material, in the first step, do not add this raw material), after mixing, put into pot steam 30 minutes form mixture stand-by;
Second step: get the distilled water of 200mL, put into pot and heat, after adding the continuous stirring of agar powder to make it fully to dissolve, add multivita-glucose simultaneously, stir and make it to be dissolved in agar solution;
The 3rd step, the agar solution of making until second step adds methyl hydroxybenzoate, methyl p-hydroxybenzoate, erythromycin, ascorbic acid and cholesterol while being cooled to 60 DEG C, (temperature of this step will be controlled at below 60 DEG C, avoids making Some Drugs to lose effect to make homogenate; , each component is mixed meanwhile, avoid causing the each position of feed nutritional labeling heterogeneity, larva ontogeny is irregular);
The 4th step: with remaining distilled water by mixture furnishing the pasty state steamed first step, pour in the homogenate that the 3rd step prepares, limit bevelling is poured in crisper after being stirred to rapidly evenly, to be cooled to gel state, open container and be placed on the interior refrigeration of 4 DEG C of refrigerators after 24 hours, make dichocrocis punctiferalis man-made feeds.These man-made feeds can in crisper, add be placed on 4 DEG C stored refrigerated for subsequent use.
The man-made feeds that utilize the present invention to make are as follows to the method for breeding of dichocrocis punctiferalis:
1) raising condition:
The whole generation all raises in growth cabinet, 23 ± 0.5 DEG C of temperature, relative humidity 75% ± 5%, photoperiod 16L:8D, intensity of illumination 3500lux.In process of the test, the contrast (CK) using the raising result of fresh corn as all feed formulas.
2) larva raising:
Larva, at diameter 12cm, is raised in the cylindrical glass bottle of high 8cm, and bottle cap is equipped with after the circular hole that 6 diameters are 1cm with card punch, welds with copper mesh.Larva hatching same day, choose gently and be equipped with in the man-made feeds vial that two length, width and height are 25mm with writing brush, 40 of every bottle graft newly hatched larvaes, 4 repetitions are established in every processing; Within every 3 days, change a feed, until all larvas all pupate.During this time, statistics survival rate of larvae and larvae development are gone through the phase.
3) raising of pupa:
The pupa on the same day is chosen gently with tweezers from vial, after weighing, put into bottle cap 50ml dactylethrae numbering with holes with electronic balance (CPA2245, Germany) weighing pupa, in growth cabinet, single head is raised to whole emergence.Record development duration and the survival rate of pupa.
4) imago breeding:
Adult is raised in rectangle dependent insect cage (350 × 270 × 250mm) with 6%～8% hydromel, and every 1-2d changes a hydromel.Meanwhile, the 3rd day from sprouting wings starts, puts into dependent insect cage, to lure Adult worms producting eggs after moistening the apple water that is surrounded by cotton gauze.During this time, every natural gift sex records adult death condition, puts into larva raising bottle and waits to hatch, until all adults are all dead after replacing ovum cloth, statistics egg laying amount on the same day on ovum cloth after mark date, numbering.
5) raising of ovum:
The worm's ovum of larva raising bottle is put in observation, adds up F1 generation larva hatching number every day, chooses, with the formula continuation raising of raising previous generation larva by hatching larva.During this time, add up larva hatching on same day number in each insect box, until no longer include larva hatching.
According to raising result, as follows to man-made feeds evaluation analysis of the present invention:
(1) development duration:
Take food the dichocrocis punctiferalis of different formulations feed, its from generation to generation development duration and larva go through extremely significantly (P<0.01) of phase difference, and pupa is gone through the phase, ovum is gone through the phase and preoviposition period difference be not very large.Wherein, it is the shortest that formula I larvae development is gone through the phase, is 20.6d; And formula I I larval phase is the longest, reach 41.7d.Full development duration is from generation to generation followed successively by formula I I, formula I II, formula I V and formula I from long to short, is respectively 63.6d, 53.3d, 49.8d and 42.3d.
The impact of four kinds of formula feeds of table 2 on dichocrocis punctiferalis development duration
Note: in table, data are mean value ± standard error, is illustrated in significant difference in 0.05 level with indicating different lowercases after data line, indicates capitalization and is illustrated in significant difference in 0.01 level (the new multipole difference analysis of DuncanShi); Table 4～5 are same.
(2) survival rate:
The survival rate of larvae that takes food formula I is the highest, being 94.5%, is secondly contrast (fresh corn), formula I V, II, III, and its survival rate of larvae is followed successively by 80.0%, 26.5%, 25.6% and 18.0%, the survival rate that formula I is described is thus higher, also exceeds 15.4% than contrast (fresh corn).
In four kinds of formula feeds, the survival rate (83.1%) that takes food the pupa of formula I is the highest, its excess-three kind is followed successively by formula I V(80.5%), formula I I(78.9%) and formula I II(44.4%), can find out that to take food the pupa survival rate of formula I the highest, only low by 6.2% than contrast (fresh corn).
Formula I (77.7%) that what egg hatching rate was the highest is, be secondly formula I V and contrast (fresh corn), be 58.2%, all the other two kinds be followed successively by formula I II(58.2%) and formula I I(16.9%); The F1 generation egg hatching rate that can find out formula I is the highest, higher by 19.5% than contrast (fresh corn).
The impact of four kinds of formula feeds of table 3 on dichocrocis punctiferalis survival rate
(3) pupa weight:
The female worm pupa heavy (0.073g) that takes food formula I is significantly higher than other formulas; Although formula I contrasts (fresh corn) low 0.001g, between the two without significant difference; Between formula I V, formula I I and formula I II, without significant difference, be respectively 0.064g, 0.060g and 0.058g.
The male worm pupa heavy (0.077g) that takes food formula I, is significantly higher than other formulas, and exceeds 0.008g than contrast (fresh corn), forms significant difference; Secondly be formula I II(0.060g) and formula I V(0.055g), formula I I(0.049g) minimum.
The heavy conspicuousness trend heavy with male worm pupa of average pupa is identical, and formula I the highest (0.075g) and contrast (fresh corn) and other equal significant differences of respectively filling a prescription.
Four kinds of formula feed impacts heavy on dichocrocis punctiferalis pupa of table 4
(4) Life of Adult and single female egg laying amount:
As can be seen from Table 5, the man-made feeds of four kinds of different amounts proportionings all have impact in various degree to dichocrocis punctiferalis Life of Adult and fecundity.Female, the male imago average life span that take food formula I II are respectively 13.2d and 23.3d, are significantly higher than other formulas.From single female egg laying amount, formula I V(107.1 grain) and formula I (97.9) although contrast (fresh corn) (112.9) lower slightly, but between the two, and with contrast between (fresh corn) all without significant difference, and apparently higher than the raising result of other formulas.
The impact of four kinds of formula feeds of table 5 on dichocrocis punctiferalis Life of Adult and single female egg laying amount
(5) dichocrocis punctiferalis population given age life table:
Obtain as calculated dichocrocis punctiferalis population given age life table (table 6).From table, parameter can be found out, what dichocrocis punctiferalis trend index of population was the highest is formula I, reaches 24.404, only low by 7.532 than contrast (fresh corn); Next is formula I V(1.790) and formula I II(1.789), formula I I is minimum, is 0.142.
For intrinsic rate of increase, the highest is also formula I, is 0.060, only low by 0.011 than contrast (fresh corn); Secondly be formula I II(0.008), other formulas are all negatives, belong to decline state, are respectively formula I V(-0.002), formula I I(-0.026).
For finite rate of increase, the highest still formula I, reaches 1.06, only low by 0.12 than contrast (fresh corn); Secondly be formula I II(1.01), formula I V(1.00) and formula I I(0.97) lower.
The life table parameters of table 6 dichocrocis punctiferalis on different formulations feed
Can be learnt by above result, the man-made feeds made from formula I are raised dichocrocis punctiferalis larva, every indexes such as its generation development duration, survival rate, heavy, the single female egg laying amount of average pupa, intrinsic rate of increase, trend index of population and finite rate of increase are all obviously better than other formulas, and the survival rate of its larva and pupa, and average pupa weight average is better than contrast (fresh corn) raising result.
The dichocrocis punctiferalis laboratory population generation survival rate of raising in view of formula I feed is high, grows neatly, and every indexes such as intrinsic rate of increase, trend index of population and finite rate of increase are all higher, thinks that formula I can be used as the preferred man-made feeds formula of raising dichocrocis punctiferalis.
The above; only for preferably detailed description of the invention of the present invention, but protection scope of the present invention is not limited to this, is anyly familiar with in technical scope that those skilled in the art disclose in the present invention; the variation that can expect easily or replacement, within all should being encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (5)
1. dichocrocis punctiferalis man-made feeds, it is characterized in that, this feed is made up of rice-chestnut powder, corn flour, peach powder, soy meal, beer yeast powder, multivita-glucose, cholesterol, casein, ascorbic acid, erythromycin, agar powder, methyl hydroxybenzoate, methyl p-hydroxybenzoate and water;
The consumption of each raw material of described feed by mass parts is: rice-chestnut powder 40, corn flour 90, peach powder 10, soy meal 30, beer yeast powder 30, multivita-glucose 10, casein 10, cholesterol 0.1, ascorbic acid 2, erythromycin 3, agar powder 10, methyl hydroxybenzoate 1, methyl p-hydroxybenzoate 2 and water 700.
2. dichocrocis punctiferalis man-made feeds according to claim 1, is characterized in that, described hydromining distilled water.
3. a preparation method for the dichocrocis punctiferalis man-made feeds as described in claim 1 to 2 any one, is characterized in that, comprises the following steps:
The first step: weigh corresponding rice-chestnut powder, corn flour, peach powder, soy meal and beer yeast powder, after mixing, put into pot steam 30 minutes form mixture stand-by;
Second step: water intaking is put into pot and heated after adding the continuous stirring of agar powder to make it fully to dissolve, adds multivita-glucose simultaneously, stirs and makes it to be dissolved in agar solution, and wherein, the consumption of water is 20 times of agar powder consumption;
The 3rd step, when the agar solution of making is cooled to 60 DEG C, adds methyl hydroxybenzoate, methyl p-hydroxybenzoate, erythromycin, ascorbic acid and cholesterol until second step, and each component mixes makes homogenate;
The 4th step: the mixture furnishing pasty state first step being made with remaining water, pour in the homogenate that the 3rd step makes, limit bevelling is poured in container after being stirred to evenly, to be cooled to gel state, open container and be placed on 4 DEG C of refrigerations after 24 hours, make the man-made feeds of dichocrocis punctiferalis.
4. the preparation method of dichocrocis punctiferalis man-made feeds according to claim 3, is characterized in that, in described the 3rd step, each component mixes process temperature and is controlled at below 60 DEG C.
5. the preparation method of dichocrocis punctiferalis man-made feeds according to claim 3, is characterized in that, described in make dichocrocis punctiferalis man-made feeds under frozen state, preserve.
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| CN104304694B (en) * | 2014-08-27 | 2017-06-06 | 河南科技大学 | Dichocrocis punctiferalis man-made feeds, preparation method and continuously rearing method |
| CN104920824B (en) * | 2015-05-18 | 2018-03-30 | 广州甘蔗糖业研究所 | Feed for raising article sugarcane borer and preparation method thereof |
| CN106212397B (en) * | 2016-10-09 | 2019-05-31 | 青岛真源生物技术有限公司 | A kind of artificial fecundation method of aphidius gifuensis |
| CN106954765B (en) * | 2017-04-11 | 2021-03-02 | 广东人为峰健康管理有限公司 | Insect feed and preparation method and application thereof |
| CN107853251B (en) * | 2017-12-07 | 2020-11-27 | 山东省果树研究所 | Method for continuously and rapidly obtaining large amount of dichocrocis punctiferalis test insects |
| CN108041353A (en) * | 2018-02-01 | 2018-05-18 | 河北省农林科学院植物保护研究所 | A kind of dichocrocis punctiferalis man-made feeds and its preparation method and application |
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| CN103110020B (en) * | 2013-03-08 | 2014-02-05 | 吉林省农业科学院 | Asiatic corn borer artificial feed containing Chinese herbal medicine additives and preparation method of artificial feed |
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