CN103250899A - Citrus fruit fly artificial-feed and preparation method thereof - Google Patents
Citrus fruit fly artificial-feed and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a citrus fruit fly artificial-feed and a preparation method of the citrus fruit fly artificial-feed. At present, no citrus fruit fly artificial-feed for controlling multiple indexes such as pupation rate, larval stage, pupal weight, female-male ratio, single-female egg laying amount and egg hatchability of citrus fruit flies is invented in domestic. The citrus fruit fly artificial-feed disclosed by the invention comprises the following components in percentage by mass: 10-15% of sucrose, 3-10% of yeast powder and 20-27% of wheat bran, and the mass sum of the sucrose, the yeast powder and the wheat bran accounts for 40% of the mass of the citrus fruit fly artificial-feed. The preparation method comprises the following steps of: mixing sucrose, yeast powder and part of a preservative; adding water into the obtained mixture, uniformly stirring the obtained product, heating the obtained product in a water bath for 20 minutes at the temperature of 70 DEG C, and stirring constantly; after the obtained object is cooled, adding the rest of the preservative and a pH regulator, and uniformly stirring the obtained mixture; and adding wheat bran into the obtained product so as to obtain a finished product. The citrus fruit fly artificial-feed disclosed by the invention is easily available in raw materials, and can control the indexes such as pupation rate, larval stage, pupal weight, female-male ratio, single-female egg laying amount and egg hatchability of citrus fruit flies.
Description
Technical field
The present invention relates to a kind of man-made feeds and preparation method thereof, especially relate to a kind of citrus fruit fly man-made feeds and preparation method thereof, belong to field of fodder.
Background technology
Citrus fruit fly is a kind of economic insects very harmful to fruits and vegetables.For studying needs ecological and Prevention Technique, since Finney successfully prepares the citrus fruit fly larva artificial feed with raw materials such as fresh carrots first, nearly 60 years of the research of its feed formula, and the substituting and raw material proportion research two aspects in the feed filler that focus mostly on.Facts have proved, desirable feed filler not only will be for the trypetid larva provides suitable feed quality, and also should solve feed nutrition effectively need lack, and infers if any research, sterol in the wheat bran is pupated most important to trypetid, and plant-feed insect does not have the ability of the synthetic sterol of oneself.So first with behind the general filler of wheat bran as citrus fruit fly and Mediterranean fruitfly larva, because of its good feeding effect, become the universal feed filler of external a plurality of authoritative departments from Tanaka.
Yet, because both at home and abroad the nutritive value between the different and different regions of wheat bran performance rating standard being existed difference, make the feed of identical proportioning exist the disunity in feed quality and the nutrition.And the basic demand that suitable feed quality and nutritional amt are desirable man-made feeds, wherein nutrient balance is particularly important, and insect's food-taking, nutrition are transformed for it and all there is bigger influence in the speed of growing; In addition, to specific insect, absorb the important prerequisite that the nutritive proportion consistent with its adaptability also is desirable man-made feeds, therefore, desirable man-made feeds will consider that not only its physical property comprises that quality, hardness, particle diameter etc. to the adaptability of insect, also must satisfy its nutritional need; Not only to perfect the feed nutrition kind, also need appropriate nutritive proportion.
Some citrus fruit fly man-made feeds are relatively preferably also arranged now, as the outstanding master thesis full-text database of China, the 1st phase in 2011,25-26,69-70 page or leaf, a kind of citrus fruit fly man-made feeds are disclosed, the ingredient requirement of these citrus fruit fly man-made feeds is more general, the not enough science of the consumption of raw material, indexs such as percentage of pupation, the larva that can not effectively control citrus fruit fly gone through the phase, pupa weight, female and male ratio, single female egg laying amount and egg hatching rate.
Also there is not a kind of raw material to be easy to get at present at home, but simulation is strong, and the citrus fruit fly man-made feeds of a plurality of indexs such as percentage of pupation, the larva that can effectively control citrus fruit fly are gone through the phase, pupa weight, female and male ratio, single female egg laying amount and egg hatching rate and preparation method thereof.
Summary of the invention
The objective of the invention is to overcome above shortcomings in the prior art, and provide a kind of raw material to be easy to get, but simulation is strong, and the citrus fruit fly man-made feeds of indexs such as percentage of pupation, the larva that can effectively control citrus fruit fly are gone through the phase, pupa weight, female and male ratio, single female egg laying amount and egg hatching rate and preparation method thereof.
The present invention addresses the above problem the technical scheme that adopts: these citrus fruit fly man-made feeds comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, its characteristics are: the quality percentage composition of described sucrose is 10-15%, the quality percentage composition of described dusty yeast is 3-10%, the quality percentage composition of described wheat bran is 20-27%, the quality sum of described sucrose, dusty yeast and wheat bran accounts for 40% of citrus fruit fly man-made feeds gross mass, and the pH value of described citrus fruit fly man-made feeds is 4-5.Make the consumption science of sucrose, dusty yeast and wheat bran among the present invention thus, can produce good reciprocation between sucrose, dusty yeast and the wheat bran, thus indexs such as percentage of pupation, the larva that can effectively control citrus fruit fly are gone through the phase, pupa weight, female and male ratio, single female egg laying amount and egg hatching rate.
As preferably, the quality percentage composition of sucrose of the present invention is 11.13%, and the quality percentage composition of described dusty yeast is 8.41%, and the quality percentage composition of described wheat bran is 20.46%.Make the pupa of the citrus fruit fly raised with these citrus fruit fly man-made feeds heavy thus, property than and the female egg laying amount of list big.
As preferably, the quality percentage composition of sucrose of the present invention is 11.04%, and the quality percentage composition of described dusty yeast is 3.27%, and the quality percentage composition of described wheat bran is 25.69%.Make that thus the cost of material of citrus fruit fly man-made feeds is low, and obtain with the citrus fruit fly that this citrus fruit fly man-made feeds are raised that can to educate the offspring many.
As preferably, the quality percentage composition of sucrose of the present invention is 12.11%, and the quality percentage composition of described dusty yeast is 3%, and the quality percentage composition of described wheat bran is 24.89%.Make that thus the cost of material of citrus fruit fly man-made feeds is low, and obtain with the citrus fruit fly that this citrus fruit fly man-made feeds are raised that can to educate the offspring many.
As preferably, the quality percentage composition of sucrose of the present invention is 14.62%, and the quality percentage composition of described dusty yeast is 3%, and the quality percentage composition of described wheat bran is 22.38%.Make that thus the cost of material of citrus fruit fly man-made feeds is low, and the egg hatching rate height of the citrus fruit fly of raising with this citrus fruit fly man-made feeds.
As preferably, the quality percentage composition of sucrose of the present invention is 15%, and the quality percentage composition of described dusty yeast is 3.13%, and the quality percentage composition of described wheat bran is 21.87%.Make with the percentage of pupation of the citrus fruit fly of this citrus fruit fly man-made feeds raising greatly thus, and that larva is gone through the phase is little.
As preferably, the quality percentage composition of sucrose of the present invention is 11%, and the quality percentage composition of described dusty yeast is 3.3%, and the quality percentage composition of described wheat bran is 25.7%.Make that thus the cost of material of citrus fruit fly man-made feeds is low, and obtain with the citrus fruit fly that this citrus fruit fly man-made feeds are raised that can to educate the offspring many.
As preferably, the quality percentage composition of sucrose of the present invention is 12.1%, and the quality percentage composition of described dusty yeast is 3%, and the quality percentage composition of described wheat bran is 24.9%.Make that thus the cost of material of citrus fruit fly man-made feeds is low, and obtain with the citrus fruit fly that this citrus fruit fly man-made feeds are raised that can to educate the offspring many.
As preferably, pH conditioning agent of the present invention is citric acid.Make that thus the adjusting function of pH conditioning agent is better among the present invention, be conducive to better promote the overall quality of citrus fruit fly man-made feeds.
The preparation method's of citrus fruit fly man-made feeds characteristics are among the present invention: the raw material of described citrus fruit fly man-made feeds comprises sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, the quality percentage composition of described sucrose is 10-15%, the quality percentage composition of described dusty yeast is 3-10%, the quality percentage composition of described wheat bran is 20-27%, and the quality sum of described sucrose, dusty yeast and wheat bran accounts for 40% of citrus fruit fly man-made feeds gross mass; Described preparation method in turn includes the following steps:
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed;
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously;
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs;
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Make that thus preparation method's the technology of the citrus fruit fly man-made feeds among the present invention is simple, processing ease, production cost is low.
The present invention compared with prior art, have the following advantages and effect: the raw material of citrus fruit fly man-made feeds is easy to get, cost of material is cheap, but simulation is strong, can take into full account citrus fruit fly larva raising quality and nutritive proportion, and illustrate reciprocation between sucrose, dusty yeast and the wheat bran in the citrus fruit fly man-made feeds, thereby by percentage of pupation, larva that this technology is effectively controlled citrus fruit fly go through the phase, indexs such as pupa weight, female and male ratio, single female egg laying amount and egg hatching rate.
Description of drawings
Fig. 1 be sucrose among the present invention, dusty yeast and wheat bran mass percent to the contour map of the percentage of pupation index in the feed evaluation index, wherein, the unit of percentage of pupation is %.
Fig. 2 is that sucrose among the present invention, dusty yeast and wheat bran mass percent are gone through index futures target contour map to the larva in the feed evaluation index, and wherein, the unit that larva is gone through the phase is the sky.
Fig. 3 is sucrose among the present invention, dusty yeast and wheat bran mass percent to the contour map of the heavy index of the pupa in the feed evaluation index, and wherein, the unit that pupa is heavy is mg.
Fig. 4 be sucrose among the present invention, dusty yeast and wheat bran mass percent to the contour map of the property in the feed evaluation index than index, wherein, the property ratio refers to female/hero.
Fig. 5 be sucrose among the present invention, dusty yeast and wheat bran mass percent to the contour map of the female egg laying amount index of the list in the feed evaluation index, wherein, the unit of single female egg laying amount be.
Fig. 6 be sucrose among the present invention, dusty yeast and wheat bran mass percent to the contour map of the egg hatching rate index in the feed evaluation index, wherein, the unit of egg hatching rate is %.
The specific embodiment
The present invention is described in further detail below in conjunction with accompanying drawing and by embodiment, and following examples are explanation of the invention and the present invention is not limited to following examples.
Embodiment 1.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 11.13%, the quality percentage composition of described dusty yeast is 8.41%, the quality percentage composition of described wheat bran is 20.46%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the mass ratio of Sodium Benzoate and methyl p-hydroxybenzoate is 1:1, and the pH conditioning agent is citric acid.
Dusty yeast used in the present embodiment is the high activity dried yeast powder, derives from Angel limited company; Sucrose is pure for analyzing, and derives from Tianjin Kermel Chemical Reagent Co., Ltd.; Methyl p-hydroxybenzoate, namely nipalgin is chemical pure, derives from chemical reagent purchase and supply 5-linked chemical plant, Shanghai; Sodium Benzoate is pure for analyzing, and derives from Shanghai Lai Ze chemical institute; Citric acid is pure for analyzing, and derives from the high brilliant Fine Chemical Co., Ltd in Hangzhou; Wheat bran is commercially available sheet products, originates from Zaozhuang, Shandong, and part is worn into powder, crosses 80 mesh sieves.
The pH value of the citrus fruit fly man-made feeds in the present embodiment is 4-5.Use the pupa of the citrus fruit fly that the citrus fruit fly man-made feeds in the present embodiment raise heavy, property than and single female egg laying amount big.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 2.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 11.04%, the quality percentage composition of dusty yeast is 3.27%, the quality percentage composition of wheat bran is 25.69%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the mass ratio of Sodium Benzoate and methyl p-hydroxybenzoate is 1:1, and the pH conditioning agent is citric acid.The pH value of the citrus fruit fly man-made feeds in the present embodiment is 4-5.The cost of material of the citrus fruit fly man-made feeds in the present embodiment is low, and obtains with the citrus fruit fly that this citrus fruit fly man-made feeds are raised that can to educate the offspring many.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 3.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 12.11%, the quality percentage composition of dusty yeast is 3%, the quality percentage composition of wheat bran is 24.89%, the quality percentage composition of the quality percentage composition water of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the mass ratio of Sodium Benzoate and methyl p-hydroxybenzoate is 1:1, and the pH conditioning agent is citric acid.The pH value of the citrus fruit fly man-made feeds in the present embodiment is 4-5.The cost of material of the citrus fruit fly man-made feeds in the present embodiment is low, and obtains with the citrus fruit fly that this citrus fruit fly man-made feeds are raised that can to educate the offspring many.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 4.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 14.62%, the quality percentage composition of dusty yeast is 3%, the quality percentage composition of wheat bran is 22.38%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the pH conditioning agent is citric acid.The pH value of citrus fruit fly man-made feeds is 4-5.The cost of material of the citrus fruit fly man-made feeds in the present embodiment is low, and the egg hatching rate height of the citrus fruit fly of raising with this citrus fruit fly man-made feeds.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 5.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 15%, the quality percentage composition of dusty yeast is 3.13%, the quality percentage composition of wheat bran is 21.87%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the pH conditioning agent is citric acid.The pH value of citrus fruit fly man-made feeds is 4-5.Use the percentage of pupation of the citrus fruit fly that the citrus fruit fly man-made feeds in the present embodiment raise big, and that larva is gone through the phase is little.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 6.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 11%, the quality percentage composition of dusty yeast is 3.3%, the quality percentage composition of wheat bran is 25.7%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the pH conditioning agent is citric acid.The pH value of citrus fruit fly man-made feeds is 4-5.Make that thus the cost of material of citrus fruit fly man-made feeds is low, and obtain with the citrus fruit fly that this citrus fruit fly man-made feeds are raised that can to educate the offspring many.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 7.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 12.1%, the quality percentage composition of dusty yeast is 3%, the quality percentage composition of wheat bran is 24.9%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the pH conditioning agent is citric acid.The pH value of citrus fruit fly man-made feeds is 4-5.Make that thus the cost of material of citrus fruit fly man-made feeds is low, and obtain with the citrus fruit fly that this citrus fruit fly man-made feeds are raised that can to educate the offspring many.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 8.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 10%, the quality percentage composition of dusty yeast is 10%, the quality percentage composition of wheat bran is 20%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the pH conditioning agent is citric acid.The pH value of citrus fruit fly man-made feeds is 4-5.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 9.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 15%, the quality percentage composition of dusty yeast is 4%, the quality percentage composition of wheat bran is 21%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the pH conditioning agent is citric acid.The pH value of citrus fruit fly man-made feeds is 4-5.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 10.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 11%, the quality percentage composition of dusty yeast is 3%, the quality percentage composition of wheat bran is 26%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the pH conditioning agent is citric acid.The pH value of citrus fruit fly man-made feeds is 4-5.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 11.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 14%, the quality percentage composition of dusty yeast is 6%, the quality percentage composition of wheat bran is 20%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the pH conditioning agent is citric acid.The pH value of citrus fruit fly man-made feeds is 4-5.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Embodiment 12.
Citrus fruit fly man-made feeds in the present embodiment comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, wherein, the quality percentage composition of sucrose is 10%, the quality percentage composition of dusty yeast is 3%, the quality percentage composition of wheat bran is 27%, the quality percentage composition of water is 58.3%, and the quality percentage composition of anticorrisive agent is that the quality percentage composition of 0.2%, pH conditioning agent is 1.5%.
Anticorrisive agent in the present embodiment is Sodium Benzoate and methyl p-hydroxybenzoate, and the pH conditioning agent is citric acid.The pH value of citrus fruit fly man-made feeds is 4-5.
In the citrus fruit fly man-made feeds of the present invention, the quality percentage composition of sucrose can be 10-15%, the quality percentage composition of dusty yeast can be 3-10%, the quality percentage composition of wheat bran can be 20-27%, but, the quality sum of sucrose, dusty yeast and wheat bran need account for 40% of citrus fruit fly man-made feeds gross mass, and namely the shared mass percent of the quality sum of sucrose, dusty yeast and wheat bran is 40%.And the consumption of water, anticorrisive agent and pH conditioning agent is common practise to those skilled in the art.
The preparation method of citrus fruit fly man-made feeds in turn includes the following steps in the present embodiment.
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed.
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously.
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs.
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
Be example with the citrus fruit fly man-made feeds among the embodiment 1-embodiment 5 below, mode by experiment verifies that citrus fruit fly man-made feeds among the present invention can control effectively that percentage of pupation, the larva of citrus fruit fly are gone through the phase, indexs such as pupa weight, female and male ratio, single female egg laying amount and egg hatching rate.
Supplying examination worm source is the citrus fruit fly experimental population in China Measures Institute adventive laboratory, this population derives from orchard, Wenling, Taizhou, take back laboratory natural host banana fruit breeding grub, (dusty yeast: sucrose=1:3) is raised adult with man-made feeds, the raising condition is T=26 ± 1 ℃, L:D=14:10, RH=75% ± 5%, and worm source, the open-air field of annual regular replenishment is to strengthen the population vigor of raising, and this population artificial feeding accumulation surpassed for 15 generations.
With sucrose, the mass percent of dusty yeast and wheat bran is variable, set bound, with reference to external universal feed prescription, make its in feed proportion and be defined as 40%, because three's variable domain there are differences, so adopt D-optimized design scheme, and with larva to the pupa ratio, larva is gone through the phase, pupa is heavy, the adult egg laying amount, adult property ratio and egg hatching rate are as the feed evaluation index, and suppose that three rank mixing multinomials are initial fitting expression, it is respectively handled proportioning and sees Table 1, final test comprises 10 regression model structure groups (model points), 9 are lost plan test set (lack of fit), amount to 19 processing, more than handle with grouping generating at random by software.
Respectively handle feed formula with reference to preparation shown in the table 1, regulate final pH value to 4.5 ± 0.2 with the 2M citric acid.Test is carried out in two batches: first is raised for a small amount of, take by weighing and respectively handle feed 10g and put in the culture dish (3cm), culture dish is put in (7 * 7 * 7cm) (lid is welded with 80 order copper mesh and is used for ventilation) in the plastic casing, each is handled and inserts first hatching larva (in the 2h) 10 is 1 repetition, amounts to 3 repetitions; In second batch is raised in batches, be layered on citrus fruit fly worm's ovum (about 200) on the wetting filter paper bar and place 100g respectively to handle feed and raise, the back is similar in said method, pupates after larva is aging, sprouts wings.
Place the artificial intelligence climate box at 26 ± 1 ℃ above-mentioned insect box, L:D=14:10, raise under RH=75% ± 5% condition, at the 6th day each processing feed and larva are layered on the gauze of cutting, gauze is placed be beneficial to mature larva on the vermiculite of absolute water content 20% and get out feed and pupate, add up the quantity of pupa in the vermiculite every day, and calculate larva and go through phase and percentage of pupation, 10 of the pupas of each batch raising acquisition of picked at random are 1 group (totally 3 groups) in addition, with putting into the EP pipe that contains above-mentioned vermiculite behind the scales/electronic balance weighing one by one, clog the mouth of pipe with cotton and treat its emergence, the adult property ratio that statistics is sprouted wings.
Get each raise in batches adult of obtaining with 5 male 5 female be that 1 group (totally 3 groups) place homemade dependent insect cage, be provided with man-made feeds (dusty yeast: sucrose=1:3) raise with the water source, rose at the 20th day and to begin to carry out the egg laying amount test, egg-laying time is 10:00-16:00, the female total amount of laying eggs of list of adding up 8 days, and measure the incubation rate of the 20th day ovum.
*Be the content of powdery wheat bran in the bracket.
The result be analyzed as follows.
1, the match of model and predictability thereof.
Table 2 and table 3 have been enumerated predicted value and the measured value of six variablees in the batch mixing test, according to above-mentioned data and through the software match, have set up each batch mixing model respectively, and it is specific as follows:
Y
Percentage of pupation=184.79A+100.30B+89.94C-214.28AB-201.27AC-58.81BC+479.15A BC-192.39AB (A-B)-187.04AC (A-C)-97.00BC (B-C)
Y
Larva is gone through the phase=9.86A+7.90B+8.24C-1.14AB+0.10AC-1.26BC+26.63ABC
Y
Pupa is heavy=18.88A+17.41B+16.56C-8.77AB-11.89AC-0.66BC-1.89ABC-4.53A B (A-B)-3.11AC (A-C)+6.33BC (B-C)
Y
Property is than (female/hero)=2.89A+3.80B+3.23C-7.39AB-8.93AC-7.78BC
Y
Single female egg laying amount=129.05A+381.23B+308.24C
Y
Egg hatching rate=132.37A+47.61B+82.90C-201.90AB-149.79AC-89.32BC+365.14AB C-326.01AB (A-B)-104.14AC (A-C)+276.50BC (B-C)
By above-mentioned model and table 4 as can be known, (A: sucrose, B: dusty yeast, C: the index all can fine match investigated of level wheat bran), its P value all reaches significance to three kinds of raw materials in the citrus fruit fly larvae feeds.In addition, lose and intend check P value all greater than 0.1, do not reach significance, this shows that this batch mixing regression model type can fully show three-component Changing Pattern, need not it is adjusted, by R
2, R
2 Adj, R
2 PredThe comprehensive correlation of judging different batch mixing models and variable is followed successively by as can be known from high to low that pupa is heavy, larva is gone through the phase, larva is to pupa ratio, single female egg laying amount, egg hatching rate and property ratio, except egg hatching rate and property than, R
2, R
2 Adj, R
2 PredBetween difference less than 0.2, according to the ratio of useful signal and noise, namely precision (Adeq precision) is all greater than 4 again, model prediction is better as can be known, this is consistent with actual result, specifically sees Table 2 and table 3.
2, the feed among the embodiment 1-embodiment 5 is to the influence of citrus fruit fly biological character.
Fig. 1 to Fig. 6 is the contour map of batch mixing model, wherein, represent percentage of pupation (%) among Fig. 1, the expression larva is gone through the phase (d) among Fig. 2, expression pupa heavy (mg) among Fig. 3, expression property is than (female/hero) among Fig. 4, the single female egg laying amount (grain) of expression among Fig. 5, represent egg hatching rate (%) among Fig. 6, the position of the corresponding three-component perpendicular bisector in each site is the concrete proportioning of feed, itself and all be fixed as 40%, with the more near point of each triangle vertex distance, its corresponding material rate is more high, otherwise it is then opposite, in the delta-shaped region of each model, all identical through the corresponding value of same isocontour feed formula, reduce gradually by being deep to shallow its corresponding isocontour parameter value according to field color.
By Fig. 1 to Fig. 6 as can be known, all there is a maximum point zone in the heavy and single female egg laying amount of pupa, and all near this apex region of dusty yeast, the feed that namely contains the high yeast concentration powder can improve the two parameter value, its theoretical prediction maximum is respectively 17.76mg, 381.22 grain, and corresponding quality of the fodder percentage composition proportioning is respectively sucrose 10.00%, dusty yeast 8.90%, wheat bran 21.10% and sucrose 10.00%, dusty yeast 10.00%, wheat bran 20.00%, go through the phase for larva, it puts high selecting near the sucrose apex region, and in the bottom of whole Delta Region, it is the low sugar zone, there is the theoretical prediction minimum of a value in it, by software as can be known its minimum of a value be 7.73d, and corresponding proportioning is sucrose 10.00%, dusty yeast 7.43%, wheat bran 22.57%, for property ratio and egg hatching rate, all there is maximum in both in two zones, when improving dusty yeast and wheat bran content, its property is increased to maximum than gradually, and namely female borer population is maximum, and minimum is positioned at the zone that takes over, center, work as sucrose, the quality percentage composition proportioning of dusty yeast and wheat bran is 12.83%, 4.55%, 22.62% o'clock, its value is 0.56, be that the male worm number is maximum, for egg hatching rate, the dusty yeast zone of high concentration and low concentration all can reach the purpose that improves incubation rate, and work as sucrose, dusty yeast and wheat bran proportioning are 11.51%, 3.00%, 25.49% o'clock, its predicted maximum is 78.25%, for percentage of pupation, there are three high value zones in it, and namely its percentage of pupation is higher when three kinds of raw materials are positioned at higher percent separately, and its predicted value can reach 100% in a plurality of ratio ranges, this ratio range sucrose, dusty yeast and wheat bran are followed successively by 10.00%-15.00%, 3.28%-9.99%, 20.00%-21.75%.
The batch mixing model also can be assessed three variablees for the influence of each evaluate parameter and the reciprocation between variable, by the regression coefficient in each model as can be known, sucrose for percentage of pupation, larva go through the phase, pupa is heavy and the having the greatest impact separately of egg hatching rate, and to the influence minimum of property ratio with single female egg laying amount, dusty yeast is for both have the greatest impact separately of back, and larva is gone through the minimum that influences of phase and egg hatching rate, heavy as for percentage of pupation and pupa, its separately influence minimum be wheat bran.Except single female egg laying amount, all there is reciprocation in three kinds of raw materials for each parameter in addition, and all reach utmost point significant difference level (
P＜0.01), go through the phase for percentage of pupation and larva, its three-component significant interaction, and the former all deposits dusty yeast and wheat bran, sucrose and dusty yeast, wheat bran and three kinds of two-component reciprocations of sucrose with the property ratio, heavy for pupa, its reciprocation only is present in the above two, egg hatching rate then exist the back both.
3, the optimization of citrus fruit fly larvae feeds proportioning.
Under test setting range condition, further optimize span (seeing table 5 for details) according to each parameter prediction value, investigation can be satisfied the desired value of above-mentioned 6 parameters, has finally obtained the prescription of 5 kinds of feeds by software and predicts the outcome accordingly, sees Table 6.Occupy inferior low and minimum in the above-described embodiments because of the cost of material among embodiment 2 and the embodiment 3 again, and institute obtains can educate the offspring and occupy second, third, so inferring embodiment 2 and embodiment 3 is comparatively ideal citrus fruit fly larvae feeds prescription, its sucrose, dusty yeast and wheat bran proportioning are respectively 11.04%, 3.27%, 25.69% and 12.11%, 3.00%, 24.89%.
4, the citrus fruit fly larvae feeds is optimized the checking of embodiment.
Feed among the acquired embodiment 1-embodiment 5 is verified with identical test method, the results are shown in Table 7, the result shows, the larva of embodiment 4 and embodiment 5 is gone through the phase maximum, and with embodiment 1-embodiment 3 significant difference of existence is arranged, the pupa representation work of embodiment 1 is higher than embodiment 2-embodiment 5, and the percentage of pupation between the feed among 5 embodiment, the property ratio, all there are not significant difference in single female egg laying amount and egg hatching rate, and embodiment 2, embodiment 3 can educate progeny size and be only second to embodiment 1, in full accord with the deduction in the 3rd, this has verified the advantage of embodiment 2 and embodiment 3 effectively.
* adopt Turkey ' the s one-factor analysis of variance, when every row letter is identical difference not significantly (
P=0.05).
Current, the application that the trypetid man-made feeds of being made up of sucrose, dusty yeast and wheat bran have been succeeded abroad, with this three for feed formula that main nutrition was constituted the U.S. used 30 surplus year, it not only satisfies larva for the growth demand of nutrition quality and quantity, also consider the feed cost of suitable feed quality and cheapness simultaneously, dusty yeast not only contains rich in protein, has also perfected simultaneously the nutrition kind of feed, abroad many with the torula powder (
Candida utilis) application more general, and wheat bran is on the basis that supplements the nutrients, further perfect physics quality and the retentiveness of feed, yet, difference by domestic and international wheat bran quality has directly caused the raising result of different regions wheat goods feed far from each other, and torula powder use cost at home is higher, therefore, there is certain necessity in the feed formula that research is fit to domestic scale raising trypetid, sucrose and dusty yeast that this research is adopted are the domestic standard product, the universal qualities that wheat bran then obtains for domestic production, the being easy to get property that its tool is good and economy, by the differentiation to brown shorts (sheet) and wheat bran powder, adopt the method for mixture experiment design, but overcome the shortcoming of external feed formula simulation difference, successfully obtained desirable forage compounding, the result proves that brown shorts and wheat bran powder ratio see Table 6 embodiment 2 and embodiment 3 at 2.3-2.6() time its wheat goods that obtain be good trypetid feedstuff.
By above-mentioned experiment as can be known, these three kinds of raw materials of sucrose, dusty yeast and wheat bran are not isolated for the effect of each growth indexes of citrus fruit fly, there is reciprocation, this phenomenon also is present in other insect feedstuff raw material, therefore, the ratio of a certain feedstuff of single change can not reach the purpose that improves feeding effect.This reciprocation has not only been illustrated in the application of mixture experiment design, also can be in whole successive range distinct forage compounding to the effect of its evaluation index.Originally the corresponding forage compounding of the ideal value zone that studies show that each feed evaluation index there are differences, sucrose proportioning as higher concentration is positioned at larva to the high point of pupa ratio, but also be positioned at the high point that larva is gone through the phase simultaneously, see Fig. 1 and Fig. 2, and there is adverse effect in the dusty yeast of low concentration to its egg laying amount and egg hatching rate, see Fig. 5 and Fig. 6, nutritional need of this hint citrus fruit fly different developmental phases exists diversity, is discrepant based on the forage compounding of different evaluation indexes.Thereby, can't multianalysis estimate the feed desired value and the feed formula that obtains, be unscientific, the application of mixture experiment design then can remedy above-mentioned defective.
Insect malesterile technique (sterile insect technique) is a kind of desirable biological control technology through facts have proved, and the prerequisite that this technology is implemented is to need male worm in batches, many methods by artificial feeding obtain abroad, yet, the resulting adult property ratio of desirable man-made feeds is usually close to 1, this has just caused the waste of female worm, and then reduced the production efficiency of male worm, and the mechanism that sex determines is also comparatively complicated, it comprises the disappearance of male parent gene, it is male that (female) is different joins, environment mechanism etc., originally studies have shown that nutritive proportion influences its correlation (R for property than also existing
2) can reach 54%, by the assessment of batch mixing model, can obtain male partially forage compounding, it is worth further research for the effect that improves Prevention Technique.
Sucrose, dusty yeast, wheat bran, water, anticorrisive agent and the used % of pH conditioning agent are the quality percentage composition among the present invention.
Though the present invention with embodiment openly as above; but it is not in order to limit protection scope of the present invention; any technical staff who is familiar with this technology not breaking away from change and the retouching of doing in the spirit and scope of the present invention, all should belong to protection scope of the present invention.
Claims (5)
1. citrus fruit fly man-made feeds, comprise sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, it is characterized in that: the quality percentage composition of described sucrose is 10-15%, the quality percentage composition of described dusty yeast is 3-10%, the quality percentage composition of described wheat bran is 20-27%, the quality sum of described sucrose, dusty yeast and wheat bran accounts for 40% of citrus fruit fly man-made feeds gross mass, and the pH value of described citrus fruit fly man-made feeds is 4-5.
2. citrus fruit fly man-made feeds according to claim 1, it is characterized in that: the quality percentage composition of described sucrose is 11.04%, and the quality percentage composition of described dusty yeast is 3.27%, and the quality percentage composition of described wheat bran is 25.69%.
3. citrus fruit fly man-made feeds according to claim 1, it is characterized in that: the quality percentage composition of described sucrose is 12.11%, and the quality percentage composition of described dusty yeast is 3%, and the quality percentage composition of described wheat bran is 24.89%.
4. citrus fruit fly man-made feeds according to claim 1, it is characterized in that: described pH conditioning agent is citric acid.
5. the preparation method of citrus fruit fly man-made feeds, it is characterized in that: the raw material of described citrus fruit fly man-made feeds comprises sucrose, dusty yeast, wheat bran, water, anticorrisive agent and pH conditioning agent, the quality percentage composition of described sucrose is 10-15%, the quality percentage composition of described dusty yeast is 3-10%, the quality percentage composition of described wheat bran is 20-27%, and the quality sum of described sucrose, dusty yeast and wheat bran accounts for 40% of citrus fruit fly man-made feeds gross mass; Described preparation method in turn includes the following steps:
Step 1: sucrose, dusty yeast and part anticorrisive agent are mixed;
Step 2: add water, stir, in 70 ℃ water-bath, heated 20 minutes then, constantly stir simultaneously;
Step 3: after the cooling, the pH conditioning agent that adds remaining anticorrisive agent and be used for regulating the pH value stirs;
Step 4: add wheat bran while stirring, mix the back and obtain the citrus fruit fly man-made feeds.
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Application publication date: 20130821 |