CN102342376A - Wheat bran medium for culturing fruit bat fly in laboratory - Google Patents
Wheat bran medium for culturing fruit bat fly in laboratory Download PDFInfo
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- CN102342376A CN102342376A CN2011101827817A CN201110182781A CN102342376A CN 102342376 A CN102342376 A CN 102342376A CN 2011101827817 A CN2011101827817 A CN 2011101827817A CN 201110182781 A CN201110182781 A CN 201110182781A CN 102342376 A CN102342376 A CN 102342376A
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Abstract
A wheat bran medium for culturing fruit bat fly in laboratory comprises 10g of wheat bran, 10 g of brown sugar, 1.2 g of agar, 1.2g of yeast and 0.15g of benzoic acid. A preparation method of the medium comprises steps of: dissolving the components in 100 ml of tap water and carrying out an autoclaving; taking glass test tube (25*200 mm) treated with dry heat sterilization (120 DEG C for 2h); subpackaging 20ml of medium into each test tube; cooling to a room temperature until the medium being solid gel; and putting fruit bat fly in the medium, wherein a test tube plug is absorbent cotton treated with dry heat sterilization. The easily prepared medium has low costs, good production effect and strong mildewing resistance, and is convenient for genetics and development biology researches on fruit bat fly.
Description
Technical field
The present invention relates to a kind of laboratory cultures fruit bat and use the wheat bran culture medium, make the fruit bat long-term surviving, with the convenient research of fruit bat being carried out aspects such as science of heredity and Developmental Biology as fruit bat food.
Background technology
Fruit bat is food with the saccharomycete, and the every matrix that can ferment may be used to raise fruit bat.Fruit bat is easy to raise, but in the laboratory cultures process, selects both had good growth result, has anti-mold effect preferably again, and is fit to the experimentize culture medium of operation of fruit bat very crucial.In genetics experiments research in the past, that raises the fruit bat employing compiles the prescription in " genetics experiments " (second edition, 1987) according to Liu Zudong, Jiang Shaohui, is mixed with maize powder medium.But preparation is complicated, and culture effect is also bad, and the reproductive capacity of fruit bat, emergence time etc. does not reach requirement.And culture medium is mouldy easily, has increased trouble to experiment.
Tradition maize powder medium prescription is, corn flour 10 grams, and sucrose 2 grams, YE 2 grams, propionic acid 0.5 ml, agar 1.5 grams are dissolved in 100 ml tap waters.
Summary of the invention
In order to overcome existing corn culture medium production performance deficiency, to be prone to mouldy shortcoming, the present invention provides a kind of novel fruit bat culture medium, wheat bran culture medium.This culture medium cost is cheap, and the pupa time of fruit bat can shift to an earlier date 24-36 hour, and the first filial generation number per dayly reaches 181.1% than maize powder medium increase preceding 5, and the anti-ability of going mouldy greatly improves.
The technical solution adopted for the present invention to solve the technical problems is: 1, adopt the main component of cheap wheat bran (wheat bran) instead of corn powder as culture medium.Say from nutritional labeling, contain the protein about 15% in the wheat bran, and corn contains protein only 8.5%.Contain trace elements such as thiamine, riboflavin, niacin in the wheat bran, also contain amylase, oxidizing ferment, peroxidase etc., these all possibly utilized by the larva of fruit bat.Say that from physical structure the wheat bran culture medium is more loose, good permeability, inside can provide sufficient oxygen and help drosophila larvae and get into the cave and ingest.2, the employing vigor is the strongest and cheap, easy purchase be that dry yeast replaces YE as nitrogenous source.Fruit bat is food with the saccharomycete, although a large amount of saccharomycete in the culture medium have lost activity through sterilization, but still is keeping visible component, and not forfeiture of nutrient content, can ingested and digests by the fruit bat larva.3, adopt steam pressure sterilization pot that culture medium is carried out autoclaving, and teat glass and tampon are carried out dry heat sterilization, under the situation that does not increase the anticorrisive agent consumption, improved the anti-ability of going mouldy of culture medium greatly.4, the place of all the other optimizations is, use cheap, but the abundant brown sugar place of sucrose of nutrition content; Propionic acid and benzoic acid all play the corrosion-resistant effect, but propionic acid is a liquid, and consumption is few, are difficult for measuring in the actual preparation, so replace with benzoic acid (dry powder).
The invention has the beneficial effects as follows that the culture medium cost of exploitation is cheap, simple and convenient preparation, production effect is good, and possesses the stronger anti-ability of going mouldy.
Description of drawings
Fig. 1 wheat bran culture medium preparation is used and laboratory cultures fruit bat sketch map
Wherein, 1: the degreasing tampon; 2: teat glass; 3: the fruit bat that is attached to the teat glass inwall; 4: prepare wheat bran culture medium in the slope.
The specific embodiment
Specify the present invention below in conjunction with accompanying drawing and through the specific embodiment.
Used instrument of the present invention and reagent are following
Wheat bran source becomes the byproduct behind the flour for wheat processing, buys from Taobao.Brown sugar is commercially available common brown sugar; Agar is available from Changxing, Qionghai City, Hainan Province agar factory (also available biochemical reagents rank agar powder replaces); Benzoic acid is available from Tianjin Da Mao chemical reagent factory (analyze pure level), dusty yeast be commercially available common be dry yeast (Yanshan Mountain board is dry yeast).
Instrument and the consumptive material used have, 50 L pressure steam sterilizers (available from the rich news company in Shanghai, YXQ-LS-50S II type), medical absorbent cotton (available from Henan Piaoan Group Co., Ltd.); Teat glass (25 * 200 millimeters reach glassware Co., Ltd available from Haian County's perseverance); Air dry oven (available from Longkou City instrument company of SAST, 101-2A type).
Embodiment
Early stage, the preparation method was:
(1) constituent of wheat bran culture medium does, wheat bran 10 grams, brown sugar 10 grams, agar 1.2 grams, yeast 1.2 grams, benzoic acid 0.15 gram.(2) take by weighing the component of capacity, all components is placed in 1000 ml beakers; (3) add 100 milliliters running water in the beaker, stir slightly; (4) beaker is put into the sterilization of 50 L pressure steam sterilizer mesohighs; (5) the pressure steam sterilizer setup parameter is 121 ℃, 20 minutes; (6) gone out and take out culture medium behind the bacterium, indoor placement got final product packing after 5 minutes.
Later stage, the preparation method was:
(1) gets an amount of teat glass (25 * 200 millimeters of specifications), and medical absorbent cotton, put in 120 ℃ of air dry ovens hot air sterilization 2 hours; (2) pour culture medium after sterilization into every teat glass 20 milliliters; (3) tilt to place glass tube (being 30 degree angles) and make into the inclined-plane, be beneficial to fruit bat and ingest with desktop; (4) indoor placement is 48 hours, and the condensation globule of test tube tube wall is volatilized naturally; (5) get a certain amount of absorbent cotton, fill in the teat glass mouth, process tampon, tampon thickness gets final product to stop fruit bat to climb out of; (6) putting into fruit bat experimentizes.
Claims (5)
1. a laboratory cultures fruit bat is used the wheat bran culture medium.
2. constituent does, wheat bran 10 grams, and brown sugar 10 grams, agar 1.2 grams, yeast 1.2 grams, benzoic acid 0.15 gram is dissolved in 100 ml tap waters, autoclaving.
3. get the teat glass (25 * 200 millimeters) of hot air sterilization (120 ℃ 2 hours) then, the culture volume packing with 20 milliliters in every test tube is cooled to room temperature, and culture medium is the solid gel shape, can put into fruit bat and cultivate.
4. the test tube stopper is the absorbent cotton that hot air sterilization crosses.
5. this culture medium cost is cheap, and preparation is simple, and production effect is good, and possesses the stronger anti-ability of going mouldy, the very convenient research of on this basis fruit bat being carried out aspects such as science of heredity and Developmental Biology.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011101827817A CN102342376A (en) | 2011-07-01 | 2011-07-01 | Wheat bran medium for culturing fruit bat fly in laboratory |
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| CN2011101827817A CN102342376A (en) | 2011-07-01 | 2011-07-01 | Wheat bran medium for culturing fruit bat fly in laboratory |
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| CN2011101827817A Pending CN102342376A (en) | 2011-07-01 | 2011-07-01 | Wheat bran medium for culturing fruit bat fly in laboratory |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630838A (en) * | 2012-04-27 | 2012-08-15 | 中国计量学院 | Preparation scheme and method of citrus fruit fly larva feed |
| CN103371277A (en) * | 2012-04-26 | 2013-10-30 | 中国科学院上海生命科学研究院 | Stable food containing gold nanoparticles |
| CN103918887A (en) * | 2014-04-22 | 2014-07-16 | 山东省农业科学院植物保护研究所 | Artificial feed for suzukii fruit bat fly |
| CN106386713A (en) * | 2016-12-15 | 2017-02-15 | 中山出入境检验检疫局检验检疫技术中心 | Incubation of cockroach egg pod and observing device |
| CN107028035A (en) * | 2017-03-22 | 2017-08-11 | 广东人为峰健康管理有限公司 | A kind of drosophila nutrient fodder and preparation method thereof |
| CN111149773A (en) * | 2020-02-17 | 2020-05-15 | 山西大学 | Drosophila resistance strain screening system |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560477A (en) * | 2008-04-16 | 2009-10-21 | 上海医药工业研究院 | Culture medium for fermenting pleocidin producing bacteria |
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2011
- 2011-07-01 CN CN2011101827817A patent/CN102342376A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560477A (en) * | 2008-04-16 | 2009-10-21 | 上海医药工业研究院 | Culture medium for fermenting pleocidin producing bacteria |
Non-Patent Citations (3)
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| 《遗传学实验》 19890630 梁彦生等 果蝇培养基 北京师范大学出版社 91 2 , * |
| 《韶关学院学报 自然科学》 20060930 彭晓云等 果蝇培养效果的影响因子分析 96-98 1,4 第27卷, 第9期 * |
| 《韶关学院学报 自然科学》 20060930 彭晓云等 果蝇培养效果的影响因子分析 96-98 2 第27卷, 第9期 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103371277A (en) * | 2012-04-26 | 2013-10-30 | 中国科学院上海生命科学研究院 | Stable food containing gold nanoparticles |
| CN103371277B (en) * | 2012-04-26 | 2014-10-22 | 中国科学院上海生命科学研究院 | Stable food containing gold nanoparticles |
| CN102630838A (en) * | 2012-04-27 | 2012-08-15 | 中国计量学院 | Preparation scheme and method of citrus fruit fly larva feed |
| CN103918887A (en) * | 2014-04-22 | 2014-07-16 | 山东省农业科学院植物保护研究所 | Artificial feed for suzukii fruit bat fly |
| CN103918887B (en) * | 2014-04-22 | 2015-12-02 | 山东省农业科学院植物保护研究所 | A kind of Ling Mushi fruit bat Prof. Du Yucang feed |
| CN106386713A (en) * | 2016-12-15 | 2017-02-15 | 中山出入境检验检疫局检验检疫技术中心 | Incubation of cockroach egg pod and observing device |
| CN107028035A (en) * | 2017-03-22 | 2017-08-11 | 广东人为峰健康管理有限公司 | A kind of drosophila nutrient fodder and preparation method thereof |
| CN111149773A (en) * | 2020-02-17 | 2020-05-15 | 山西大学 | Drosophila resistance strain screening system |
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Application publication date: 20120208 |