CN103371277B - Stable food containing gold nanoparticles - Google Patents
Stable food containing gold nanoparticles Download PDFInfo
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- CN103371277B CN103371277B CN201210127264.4A CN201210127264A CN103371277B CN 103371277 B CN103371277 B CN 103371277B CN 201210127264 A CN201210127264 A CN 201210127264A CN 103371277 B CN103371277 B CN 103371277B
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Abstract
The invention discloses a stable food containing gold nanoparticles. The food comprises gold nanoparticles and propionic acid. The invention also discloses a preparation method and application of the food containing the gold nanoparticles. According to the food, the gold nanoparticles are uniformly dispersed in the food so as to be stably absorbed by animal tissues, the lipid metabolism is improved, the activity of an Akt signal channel is improved, the food can be used for feeding animals, the fat content of the animals is improved, and the food can be used for preparing compounds loading drugs or nucleic acid and lays a foundation for research of further researching biological effects of nanogold loaded drugs or nucleic acid in animal bodies.
Description
Technical field
The present invention relates to a kind of food, especially animal food, is specifically related to a kind of stable food that comprises nanogold particle.
Background technology
Nano material, mean the natural or artificial material of diameter between 1-100nm, because its size is similar to most of biomolecule, can freely pass through cell membrane, and easily carry a large amount of medicines or biomolecule, therefore the biological study based on nano material is at (the Daniel that developed by leaps and bounds in recent ten years, M.C. & Astruc, D.Gold nanoparticles:assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology.Chem Rev 104, 293-346, 2004).
Nm of gold is as the nano material being employed the earliest, and its history can be traced back to the ancient Egypt before several thousand.Alchemists will just be contained nm of gold in the Jinsui River of drinking for Pharaoh of making after gold fusing.And also popular this method in medieval European noble, and be used to treat gout or arthritis.And research in recent years shows, this therapy molecule mechanism is behind that nm of gold is for the interference of inflammation signal path (the .Arthritis Rheum 56 (2) such as Amelioration of collagen-induced arthritis in rats by nanogold.Tsai CY, 544-54,2007).Because nm of gold is less to the toxicity of cell; carry drug molecule or specific nucleic acid molecule (comprising antisense DNA or siRNA) taking nm of gold as carrier and enter cell; the strategy that carries out (comprising mRNA and protein) targeted therapy for abasement gene product has extraordinary application prospect (Rosi; N.L. wait .Oligonucleotide-modified gold nanoparticles for intracellular gene regulation.Science 312; 1027-1030,2006).
In vertebrate, import at present nm of gold carrier and mainly take intravenous method, be that nm of gold is arrived whole body and absorbed (Boisselier E & Astruc D.Gold nanoparticles in nanomedicine:preparations by target cell by blood circulation system, imaging, diagnostics, therapies and toxicity.Chem Soc Rev 38 (6), 1759-82, (2009)).But,, cannot inject because it is small-sized as fruit bat for model animal, cause very large difficulty for the effect of studying nm of gold in fruit bat body.
Recently, after having researcher that other two kinds of nano materials (Nano Silver and CNT) are infiltrated to fruit bat food, feed fruit bat, find that this bi-material can be absorbed (the .Silver nanoparticles induced heat shock protein 70 such as Ahamed M by fruit bat tissue, oxidative stress and apoptosis in Drosophila melanogaster.Toxicol Appl Pharmacol 242 (3), 263-9,2010; The .Single-walled carbon nanotubes in the intact organism:near-IR imaging and biocompatibility studies in Drosophila.Nano Lett 7 (9) such as Leeuw TK, 2650-4,2007).But this method has obvious defect, can not ensure the homogeneity of nano material concentration in food, cannot do the research quantizing to the vivo effect of nano material.In order to make nano material in fruit bat food there is the concentration of homogeneous, need to be before foodstuff coagulation and nano material premix, but nano material is difficult to stand the test of high temperature conventionally, cause the physical property of material to change (for example nano material can be assembled), thus further research of impact.
Therefore, still need that development is a kind of to be applicable to oral and nano material and to be uniformly distributed food therein.
Summary of the invention
The object of the present invention is to provide a kind of stable food that comprises nanogold particle, can ensure the homogeneity of nanogold particle concentration in food.
A first aspect of the present invention, provides a kind of food, contains nanogold particle and 0.1wt%-3wt% propionic acid, by the gross weight of food in described food.
In another preference, described propionic acid is both as anticorrisive agent, also as the agglutination inhibitor that suppresses nanogold particle gathering.
In another preference, described food has following one or more characteristics:
(a) stability: preserve under room temperature after 5 days nm of gold clustering phenomena does not occur;
(b) anti-corrosive properties: preserve under room temperature after 5 days and do not occur food spoilage.
In another preference, the concentration of described nanogold particle is 0.1-10nM.
In another preference, the concentration of described nanogold particle is 0.5-5nM, preferably, is 0.5-2nM.
In another preference, by the gross weight of food, the concentration of described nanogold particle is 2.4-240 μ g/ml, preferably, is 12-192 μ g/ml.
In another preference, by the gross weight of food, the concentration of described nanogold particle is 12-120 μ g/ml, preferably, is 12-48 μ g/ml.
The average grain diameter of described nanogold particle is 1-100nm.
Preferably, the average grain diameter of described nanogold particle is 5-60nm, and more preferably, the average grain diameter of described nanogold particle is 10-40nm.
In another preference, described nanogold particle is evenly distributed in described food.
In another preference, described nanogold particle comprises nanosphere, nanometer rods, nano wire, nanobelt.
In another preference, the content of described propionic acid is 0.25wt%-1.5wt%.
In another preference, the content of described propionic acid is 0.5wt%-1wt%.
In another preference, the adsorption of described nanogold particle has albumen.
In another preference, described albumen is bovine serum albumin(BSA), or oralbumin.
A second aspect of the present invention, provides a kind of composition, and described composition comprises:
(a) food described in first aspect;
(b) active component, wherein,
Described active constituent loading is on described nanogold particle.
In another preference, described active component is medicine or nucleic acid.
A third aspect of the present invention, provides the preparation method of the food described in first aspect, comprises the following steps:
(a) propionic acid is joined and in the animal food not condensing, obtain food first product;
(b) nano-Au solution is added in the described food first product that step (a) obtains, obtains food claimed in claim 1.
In another preference, in described step (b), mixing temperature is 50-55 DEG C.
In another preference, adopt albumen step that nanogold particle is processed front also comprising in described step (b):
(b0) adopt the protein solution of 0.1-100 μ g/ml to process described nanogold particle 5-30min, make the adsorption albumen of nanogold particle.
In another preference, described albumen is bovine serum albumin(BSA), or oralbumin.
In another preference, described protein concentration is 0.1-25 μ g/ml, preferably, is 2-10 μ g/ml.
In another preference, described animal food is the food of fruit bat food, C. Elegans Automatic Screening food, rabbit, the food of dog, food, mouse food or the rat food of pig.
A fourth aspect of the present invention, provides the purposes of the food described in first aspect, is selected from lower group:
(a) for letting animals feed;
(b) for improving the fat content of animal;
(c) for the preparation of the composition of carrying medicament or nucleic acid.
Food of the present invention, nanogold particle is evenly dispersed in food, can stably be absorbed by animal tissue, change fat metabolism, improve the activity of Akt signal path, can, for letting animals feed, improve the fat content of animal, also can be for the preparation of the composition of carrying medicament or nucleic acid, for further research nm of gold carrying medicament or nucleic acid in animal body the research of biological effect lay the foundation.
In should be understood that within the scope of the present invention, above-mentioned each technical characterictic of the present invention and can combining mutually between specifically described each technical characterictic in below (eg embodiment), thus form new or preferred technical scheme.As space is limited, tire out and state no longer one by one at this.
Detailed description of the invention
Present inventor is through extensive and deep research, and unexpected discovery, under propionic acid exists, mixes nanogold particle with animal food, and nanogold particle can be evenly dispersed in food, is difficult for assembling.On this basis, completed the present invention.
Term
Nanogold particle (also claiming nm of gold)
Nanogold particle refers to the gold particle of size within the scope of 1-100nm, has high electron density, dielectric property and catalytic action, can be combined with multiple large biological molecule, and not affect its biologically active.
The preparation method of nanogold particle mainly adopts reducing agent, as natrium citricum, sodium borohydride etc., and reduction gold chloride.In preparation process, by controlling reaction condition, reagent dosage, can obtain the good gold grain of monodispersity.Nanogold particle used herein can adopt any known method in prior art to prepare.
Nanogold particle with-SH ,-NH
2,-the groups such as CH have very strong affinity.These character make gold grain have the application of special biochemical and medical science aspect.Gold grain is connected by covalent bond with the oligonucleotides of alkyl modified, and composition nanometer bio probe, can be used for the detection of DNA sequence dna; Nm of gold carrying antisense DNA or s iRNA are combined with the mRNA of target gene after entering cell, can suppress the expression of target gene.Gold grain can also be connected with protein, as being connected with specific antibodies, detects its corresponding antigen.In vivo, the gold grain that carries medicine and specific antibodies (as the antibody of cancer cell surface antigen) can enter target cell specifically, by growth, metabolism and the differentiation etc. of the drug influence target cell of carrying.Use gold grain to carry out DNA or protein detection as probe, not only simple to operate, phenomenon obviously but also the degree of accuracy, highly sensitive; Carry nucleic acid or medicine taking gold grain as carrier and there is high efficiency and specificity.In addition, gold grain itself also has impact to cell activities.Gold grain can change its Acidity of Aikalinity after entering lysosome, thereby affects cell autophagy.
Universal method
Nanogold particle is assembled the detection of situation:
Nano-Au solution is red, and after occurring to assemble, color transition is blue.After food mixes with nm of gold monomer, because the impact of yeast color is rufous, as color changes, can think and assemble.
If color does not change, the food that comprises nanogold particle is placed after 5 days in room temperature, whether the nanogold particle that adopts transmission electron microscope to detect in food there is a small amount of gathering.Under room temperature, preserve after 5 days nm of gold clustering phenomena does not occur, can think that the food that comprises nanogold particle is stable.
Food (food)
The invention provides a kind of novel food, in described food, contain nanogold particle and 0.1wt%-3wt% propionic acid, by the gross weight of food.
In another preference, described propionic acid is both as anticorrisive agent, also as the agglutination inhibitor that suppresses nanogold particle gathering.
In another preference, described food has following one or more characteristics:
(a) stability: preserve under room temperature after 5 days nm of gold clustering phenomena does not occur;
(b) anti-corrosive properties: preserve under room temperature after 5 days and do not occur food spoilage.
In another preference, the concentration of described nanogold particle is 0.1-10nM.
In another preference, the concentration of described nanogold particle is 0.5-5nM, preferably, is 0.5-2nM.
In another preference, by the gross weight of food, the concentration of described nanogold particle is 2.4-240 μ g/ml, preferably, is 12-192 μ g/ml.
In another preference, by the gross weight of food, the concentration of described nanogold particle is 12-120 μ g/ml, preferably, is 12-48 μ g/ml.
The average grain diameter of described nanogold particle is 1-100nm.
Preferably, the average grain diameter of described nanogold particle is 5-60nm, and more preferably, the average grain diameter of described nanogold particle is 10-40nm.
In another preference, described nanogold particle is evenly distributed in described food.
In another preference, described nanogold particle comprises nanosphere, nanometer rods, nano wire, nanobelt.
In another preference, the content of described propionic acid is 0.25wt%-1.5wt%.
In another preference, the content of described propionic acid is 0.5wt%-1wt%.
In another preference, the adsorption of described nanogold particle has albumen.
In another preference, described albumen is bovine serum albumin(BSA), or oralbumin.
In another preference, food of the present invention comprises animal food, nanogold particle and 0.1wt%-3wt% propionic acid, by the gross weight of food.
Animal self can not be manufactured organic matter, must obtain food from the external world, to obtain growth desired nutritional and energy.As used herein, term " animal food ", " animal foodstuff " or " animal feed " have identical implication, all refer to the food of letting animals feed.As in laboratory research, raise the food of model animal pig, dog, rabbit, mouse, rat etc.The formula of this food can adopt food preparation known in the art.
As the formula of fruit bat food and compound method as follows:
10wt% dusty yeast, 10wt% sucrose, 1.5wt% agar powder, anticorrisive agent (0.1wt% Sodium Benzoate, or 0.025wt% methyl hydroxybenzoate, or 0.7wt% propionic acid) surplus is water.
Dusty yeast, sucrose, agar powder are added to the water, and after boiling, are cooled to 60-65 DEG C, add anticorrisive agent.
Wherein, propionic acid is the carboxylic acid of three carbon, and chemical formula is CH
3cH
2cOOH.Pure propionic acid is colourless, corrosive liquid, with penetrating odor, can be used as esterifying agent, solvent, plasticizer, emulsifying agent, herbicide, can also be used for preparing food, as anticorrisive agent.
Formula and the compound method of C. Elegans Automatic Screening food are as follows:
1.7wt% agar, 0.3wt%NaCl, 0.25wt% peptone, 5mg/L cholesterol, 1mM calcium chloride, 1mM magnesium sulfate, 25mM potassium phosphate, surplus is water.
Agar, NaCl, peptone, cholesterol is added to the water, and after boiling, is cooled to 60-65 DEG C, adds calcium chloride, magnesium sulfate, potassium phosphate, then cooling condensing.Before use, smear Escherichia coli.
Mouse feed formula: corn flour 27wt%, wheat bran 19wt%, rice 16wt%, soya-bean cake 16wt%, fish meal 13wt%, calcium powder 3wt%, bone meal 3wt%, dusty yeast 2.3wt%, salt 0.5wt%, B B-complex 0.1wt%, micro-0.1wt%.
Composition
Composition of the present invention, comprises aforementioned food and active component, and wherein said active constituent loading is on described nanogold particle.
Composition of the present invention is food compositions or composite medicine.
As used herein, term " active component " is medicine or nucleic acid, can adopt the known method of prior art to load on nanogold particle.
Composition of the present invention, enters in animal body by oral route.
Preparation method
The preparation method of food of the present invention, comprises the following steps:
(a) propionic acid is joined and in the animal food not condensing, obtain food first product;
(b) nano-Au solution is added in the described food first product that step (a) obtains, obtains food claimed in claim 1.
In another preference, in described step (b), nano-Au solution is added in the described food first product that step (a) obtains, the final concentration of described nm of gold is 0.1-10nM.Being preferably 0.5-5nM, more preferably, is 0.5-2nM.
In another preference, in described step (b), mixing temperature is 50-55 DEG C.
In another preference, the front step that adopts albumen to process nanogold particle that also comprises of described step (b):
(b0) adopt the protein solution of 0.1-100 μ g/ml to process described nanogold particle 5-30min, make the adsorption albumen of nanogold particle.
In another preference, described albumen is bovine serum albumin(BSA), or oralbumin.
In another preference, described protein concentration is 0.1-25 μ g/ml, preferably, is 2-10 μ g/ml.
Described animal food is the food of fruit bat food, C. Elegans Automatic Screening food, rabbit, the food of dog, food, mouse food or the rat food of pig.Can adopt the whole bag of tricks preparation known in the art.
Purposes
Food of the present invention, can be for letting animals feed.
In addition, food of the present invention, also can be used for improving the fat content of animal.
Further, food of the present invention, can be for the preparation of the composition of carrying medicament or nucleic acid.
In another preference, described animal is fruit bat, Samsonite nematode, mouse, rat.
The above-mentioned feature that the present invention mentions, or the feature that embodiment mentions can be combined.All features that this case description discloses can with any composition forms use, each feature disclosing in description, can be replaced by any alternative characteristics that identical, impartial or similar object are provided.Therefore apart from special instruction, the feature disclosing is only the general example of equalization or similar features.
Major advantage of the present invention is:
(1) provide a kind of novel animal food.
(2) animal food provided by the invention, nm of gold is dispersed in animal food, and concentration is all difficult for assembling with, nanogold particle everywhere.
(3) the invention provides a kind of preparation method of food of nano-golden particle-containing.
(4) lay the foundation for nano material or the research in vivo of medicine carrying/nucleic acid nano material.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.The experimental technique of unreceipted actual conditions in the following example, the condition of conventionally advising according to normal condition or according to manufacturer.
Unless otherwise defined, the familiar meaning of all specialties that use in literary composition and scientific words and one skilled in the art is identical.In addition, any method similar or impartial to described content and material all can be applicable in the inventive method.The use that better implementation method described in literary composition and material only present a demonstration.
Embodiment 1
The preparation of nano-Au solution
The gold chloride reducing process of employing standard adds gold chloride in boiling water, adds rapidly natrium citricum under the condition of vigorous stirring, the nano-Au solution mother liquor of cooling rear formation 20nM.
Embodiment 2
The pretreatment of nanogold particle
2a: be 1: 200 according to volume ratio, 100 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in nano-Au solution prepared by embodiment 1, the final concentration that makes bovine serum albumin(BSA) is the 0.5 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min, makes nm of gold adsorption BSA.
2b: be 1: 200 according to volume ratio, 200 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in nano-Au solution prepared by embodiment 1, the final concentration that makes bovine serum albumin(BSA) is the 1 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min, makes nm of gold adsorption BSA.
2c: be 1: 200 according to volume ratio, 400 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in nano-Au solution prepared by embodiment 1, the final concentration that makes bovine serum albumin(BSA) is the 2 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min, makes nm of gold adsorption BSA.
2d: be 1: 200 according to volume ratio, 600 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in nano-Au solution prepared by embodiment 1, the final concentration that makes bovine serum albumin(BSA) is the 3 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min, makes nm of gold adsorption BSA.
2e: be 1: 200 according to volume ratio, 1000 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in nano-Au solution prepared by embodiment 1, the final concentration that makes bovine serum albumin(BSA) is the 5 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min, makes nm of gold adsorption BSA.
2f: be 1: 200 according to volume ratio, 1400 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in nano-Au solution prepared by embodiment 1, the final concentration that makes bovine serum albumin(BSA) is the 7 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min, makes nm of gold adsorption BSA.
2g: be 1: 200 according to volume ratio, in the nano-Au solution that 2000 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are prepared to embodiment 1 with 1: 200 dilution proportion, the final concentration that makes bovine serum albumin(BSA) is the 10 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min, makes nm of gold adsorption BSA.
Embodiment 3
The preparation of food
Preparation fruit bat food, being cooled to 50-55 DEG C adds respectively in proportion through embodiment 2a～2g and processes the nm of gold of the adsorption BSA obtaining, the nano-Au solution that embodiment 1 obtains, mix, the final concentration of nanogold particle in food is 2nM, the content of anticorrisive agent propionic acid is 0.7wt%, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
Preparation fruit bat food, being cooled to 48 DEG C adds respectively in proportion through embodiment 2a～2g and processes the nm of gold of the adsorption BSA obtaining, the nano-Au solution that embodiment 1 obtains, mix, the final concentration of nanogold particle in food is 2nM, the content of anticorrisive agent propionic acid is 0.7wt%, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
Preparation fruit bat food, being cooled to 60 DEG C adds respectively in proportion through embodiment 2a～2g and processes the nm of gold of the adsorption BSA obtaining, the nano-Au solution that embodiment 1 obtains, mix, the final concentration of nanogold particle in food is 2nM, the content of anticorrisive agent propionic acid is 0.7wt%, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
Table 1 temperature and the BSA impact on stability of nano gold
The result of table 1 shows, concentration >=0.5 μ g/ml BSA, and after especially nm of gold being wrapped up in concentration >=2 μ g/ml BSA, the stability to nm of gold in food has facilitation.Between 50-55 DEG C, nm of gold is mixed with the fruit bat food not condensing, can prevent the gathering of nm of gold.At the temperature higher than 55 DEG C, mix nm of gold and fruit bat food, nm of gold is easily assembled.At the temperature lower than 50 DEG C, fruit bat food condenses rapidly, and nm of gold is not easy to mix with food.
Embodiment 4
The preparation of food
4a: at 60 DEG C, propionic acid is joined in fruit bat food, be cooled to 50-55 DEG C and add in proportion the nm of gold of processing the adsorption BSA obtaining through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, and the content of propionic acid is 0.7wt%.
4b: methyl hydroxybenzoate is joined in fruit bat food at 60 DEG C, be cooled to 50-55 DEG C and add in proportion the nm of gold of processing the adsorption BSA obtaining through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, and the content of methyl hydroxybenzoate is 0.025wt%.
4c: Sodium Benzoate is joined in fruit bat food at 60 DEG C, be cooled to 50-55 DEG C and add in proportion the nm of gold of processing the adsorption BSA obtaining through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, and the content of Sodium Benzoate is 0.1wt%.
4d: propionic acid and methyl hydroxybenzoate are joined in fruit bat food at 60 DEG C, be cooled to 50-55 DEG C and add in proportion the nm of gold of processing the adsorption BSA obtaining through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, the content of propionic acid is 0.7wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
4e: propionic acid and Sodium Benzoate are joined in fruit bat food at 60 DEG C, be cooled to 50-55 DEG C and add in proportion the nm of gold of processing the adsorption BSA obtaining through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, the content of propionic acid is 0.7wt%, and the content of Sodium Benzoate is 0.1wt%.
4f: Sodium Benzoate and methyl hydroxybenzoate are joined in fruit bat food at 60 DEG C, be cooled to 50-55 DEG C and add in proportion the nm of gold of processing the adsorption BSA obtaining through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
4g: propionic acid, methyl hydroxybenzoate and Sodium Benzoate are joined in fruit bat food at 60 DEG C, be cooled to 50-55 DEG C and add in proportion the nm of gold of processing the adsorption BSA obtaining through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, the content of propionic acid is 0.7wt%, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
4h: do not add anticorrisive agent, fruit bat food is cooled to 50-55 DEG C and adds in proportion the nm of gold of processing the adsorption BSA obtaining through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM.
The impact of table 2 anticorrisive agent on stability of nano gold
| Embodiment | 4a | 4b | 4c | 4d | 4e | 4f | 4g | 4h |
| Sodium Benzoate | - | - | + | - | + | + | + | - |
| Methyl hydroxybenzoate | - | + | - | + | - | + | + | - |
| Propionic acid | + | - | - | + | + | - | + | - |
| After 2 days, whether assemble | No | Be | Be | No | No | Be | Be | No |
| After 5 days, whether assemble | No | Be | Be | Be | Be | Be | Be | Be |
Wherein ,+represent to add ,-represent not add
Table 2 result shows, Sodium Benzoate and methyl hydroxybenzoate can be promoted the gathering of nm of gold in food.While only adding propionic acid, it is constant that the food that comprises nm of gold still maintained color after 5 days, detects nanogold particle do not assemble through transmission electron microscope.What is interesting is, even if do not add any anticorrisive agent, nm of gold still can be assembled after 5 days, illustrated that propionic acid brought into play the function outside anticorrisive agent, prevented that nm of gold from assembling in food.
Embodiment 5
The preparation of food
5a: basic identical with the method for embodiment 4a, difference is only, the final concentration of nanogold particle in food is 0.5nM.
5b: basic identical with the method for embodiment 4a, difference is only, the final concentration of nanogold particle in food is 1nM.
5c: basic identical with the method for embodiment 4a, difference is only, the final concentration of nanogold particle in food is 3nM.
5d: basic identical with the method for embodiment 4a, difference is only, the final concentration of nanogold particle in food is 5nM.
5e: basic identical with the method for embodiment 4a, difference is only, the content of propionic acid is 0.5wt%.
5f: basic identical with the method for embodiment 4a, difference is only, the content of propionic acid is 1wt%.
5g: basic identical with the method for embodiment 4a, difference is only, the content of propionic acid is 1.5wt%.
5h: basic identical with the method for embodiment 4a, difference is only, adds the nm of gold of processing the adsorption BSA obtaining through embodiment 2e.
5i: basic identical with the method for embodiment 4a, difference is only, adds the nm of gold of processing the adsorption BSA obtaining through embodiment 2g.
5j: basic identical with the method for embodiment 4a, difference is only, propionic acid is joined in the food of C. Elegans Automatic Screening.
Result shows, in food prepared by embodiment 5a-5j, nm of gold is assembled.
Embodiment 6
In body, take in research
Adopt food prepared by embodiment 4a to feed fruit bat, allow drosophila larvae grow on food.After 4 days, separate the organ (epidermis, intestines, fat-body, imaginal discs, brain and tracheae) of fruit bat, adopt the picked-up situation of I transmission electron microscope observing or detection nm of gold in individual organ.
Result shows, nm of gold can be taken in and absorb by fruit bat, mainly be distributed in epidermis, intestines and fat-body, distributes less in imaginal discs, brain and tracheae.
Embodiment 7
Internal metabolism research
Adopt food prepared by embodiment 4a to feed fruit bat, allow drosophila larvae grow on food.
Taking the food of plus nano gold not as control group.
After 4 days, after the cracking of employing fruit bat, measure fat content with surveying fat kit (Sigma).
Compare with contrasting fruit bat, its fat content rises, and detects the Akt content of activation through Western Blot, finds to rise with the activity of the closely-related Akt signal path of fruit bat fat metabolism, illustrates after nm of gold enters fruit bat tissue and can change fat metabolism.
Above result shows, the food that comprises nm of gold of preparing through the inventive method, not only has stable physical property, does not assemble, the more important thing is and can effectively nm of gold be imported in fruit bat body, for nm of gold carrier band medicine or nucleic acid molecules, the research in fruit bat body is laid a good foundation.
All documents of mentioning in the present invention are all quoted as a reference in this application, are just quoted separately as a reference as each section of document.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read above-mentioned instruction content of the present invention, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (10)
1. a food, is characterized in that, contains nanogold particle and 0.1wt%-3wt% propionic acid, by the gross weight of food in described food; Wherein,
The concentration of described nanogold particle is 0.5-5nM;
And the adsorption of described nanogold particle has albumen.
2. food as claimed in claim 1, is characterized in that, the concentration of described nanogold particle is 0.5-2nM.
3. food as claimed in claim 1, is characterized in that, the content of described propionic acid is 0.25wt%-1.5wt%.
4. food as claimed in claim 1, is characterized in that, adopts the protein solution of 0.1-100 μ g/ml to process described nanogold particle 5-30min, makes the adsorption of described nanogold particle have albumen.
5. a composition, is characterized in that, described composition comprises:
(a) food claimed in claim 1;
(b) active component, wherein,
Described active constituent loading is on described nanogold particle.
6. composition as claimed in claim 5, is characterized in that, described active component is medicine or nucleic acid.
7. the preparation method of food as claimed in claim 1, is characterized in that, said method comprising the steps of:
(a) propionic acid is joined and in the animal food not condensing, obtain food first product;
(b) nano-Au solution is added in the described food first product that step (a) obtains, obtains food claimed in claim 1.
8. preparation method as claimed in claim 7, is characterized in that, adopts albumen step that nanogold particle is processed front also comprising in described step (b):
(b0) adopt the protein solution of 0.1-100 μ g/ml to process described nanogold particle 5-30min, make the adsorption albumen of nanogold particle.
9. preparation method as claimed in claim 7, is characterized in that, described animal food is the food of fruit bat food, C. Elegans Automatic Screening food, rabbit, the food of dog, food, mouse food or the rat food of pig.
10. the purposes of food as claimed in claim 1, is characterized in that, described purposes is selected from lower group:
(a) for letting animals feed;
(b) for improving the fat content of animal;
(c) for the preparation of the composition of carrying medicament or nucleic acid.
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