CN103371277A - Stable food containing gold nanoparticles - Google Patents
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- CN103371277A CN103371277A CN2012101272644A CN201210127264A CN103371277A CN 103371277 A CN103371277 A CN 103371277A CN 2012101272644 A CN2012101272644 A CN 2012101272644A CN 201210127264 A CN201210127264 A CN 201210127264A CN 103371277 A CN103371277 A CN 103371277A
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Abstract
The invention discloses a stable food containing gold nanoparticles. The food comprises gold nanoparticles and propionic acid. The invention also discloses a preparation method and application of the food containing the gold nanoparticles. According to the food, the gold nanoparticles are uniformly dispersed in the food so as to be stably absorbed by animal tissues, the lipid metabolism is improved, the activity of an Akt signal channel is improved, the food can be used for feeding animals, the fat content of the animals is improved, and the food can be used for preparing compounds loading drugs or nucleic acid and lays a foundation for research of further researching biological effects of nanogold loaded drugs or nucleic acid in animal bodies.
Description
Technical field
The present invention relates to a kind of food, especially animal food is specifically related to a kind of stable food that comprises nanogold particle.
Background technology
Nano material, mean the natural or artificial material of diameter between 1-100nm, because its size is similar to most of biomolecule, can freely pass through cell membrane, and carry easily a large amount of medicines or biomolecule, therefore based on the biological study of nano material at (Daniel, the M.C.﹠amp of being developed by leaps and bounds in recent ten years; Astruc, D.Gold nanoparticles:assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology.Chem Rev 104,293-346,2004).
Nm of gold is as the nano material that is employed the earliest, and its history can be traced back to the ancient Egypt before several thousand.Just contain nm of gold in Jinsui River that supplies the Pharaoh to drink that alchemists make after gold is melted.And also popular this method among the medieval European noble, and be used to treat gout or arthritis.And studies show that in recent years, this therapy molecule mechanism behind is that nm of gold is for the interference of inflammation signal path (the .Arthritis Rheum 56 (2) such as Amelioration of collagen-induced arthritis in rats by nanogold.Tsai CY, 544-54,2007).Because nm of gold is less to the toxicity of cell; carry drug molecule or specific nucleic acid molecule (comprising antisense DNA or siRNA) take nm of gold as carrier and enter cell; the strategy that carries out (comprising mRNA and protein) targeted therapy for abasement gene product has extraordinary application prospect (Rosi; N.L. wait .Oligonucleotide-modified gold nanoparticles for intracellular gene regulation.Science 312; 1027-1030,2006).
Import at present the nm of gold carrier and mainly take intravenous method in vertebrate, namely nm of gold arrives whole body and is absorbed (Boisselier E ﹠amp by target cell by blood circulation system; Astruc D.Gold nanoparticles in nanomedicine:preparations, imaging, diagnostics, therapies and toxicity.Chem Soc Rev 38 (6), 1759-82, (2009)).But for model animal such as fruit bat, because it is small-sized, can't inject, cause very large difficulty for the effect of in the fruit bat body, studying nm of gold.
Recently, feed fruit bat after having the researcher that other two kinds of nano materials (Nano Silver and CNT) are infiltrated fruit bat food, find that this bi-material can be absorbed by the fruit bat tissue (the .Silver nanoparticles induced heat shock protein 70 such as Ahamed M, oxidative stress and apoptosis in Drosophila melanogaster.Toxicol Appl Pharmacol 242 (3), 263-9,2010; The .Single-walled carbon nanotubes in the intact organism:near-IR imaging and biocompatibility studies in Drosophila.Nano Lett 7 (9) such as Leeuw TK, 2650-4,2007).But this method has obvious defective, namely can not guarantee the homogeneity of nano material concentration in food, can't do to the vivo effect of nano material the research of quantification.In order to make the nano material in the fruit bat food have the concentration of homogeneous, need to be before foodstuff coagulation and the nano material premix, but nano material is difficult to stand the test of high temperature usually, cause the physical property of material to change (for example nano material can be assembled), thus further research of impact.
Therefore, still need that development is a kind of to be fit to oral and nano material and to be evenly distributed on wherein food.
Summary of the invention
The object of the present invention is to provide a kind of stable food that comprises nanogold particle, can guarantee the homogeneity of nanogold particle concentration in food.
A first aspect of the present invention provides a kind of food, contains nanogold particle and 0.1wt%-3wt% propionic acid in the described food, by the gross weight of food.
In another preference, described propionic acid is both as anticorrisive agent, also as the agglutination inhibitor that suppresses the nanogold particle gathering.
In another preference, described food has following one or more characteristics:
(a) stability: the nm of gold clustering phenomena did not occur after 5 days in preservation under the room temperature;
(b) anti-corrosive properties: preservation food spoilage do not occur after 5 days under the room temperature.
In another preference, the concentration of described nanogold particle is 0.1-10nM.
In another preference, the concentration of described nanogold particle is 0.5-5nM, preferably, is 0.5-2nM.
In another preference, by the gross weight of food, the concentration of described nanogold particle is 2.4-240 μ g/ml, preferably, is 12-192 μ g/ml.
In another preference, by the gross weight of food, the concentration of described nanogold particle is 12-120 μ g/ml, preferably, is 12-48 μ g/ml.
The average grain diameter of described nanogold particle is 1-100nm.
Preferably, the average grain diameter of described nanogold particle is 5-60nm, and more preferably, the average grain diameter of described nanogold particle is 10-40nm.
In another preference, described nanogold particle is evenly distributed in the described food.
In another preference, described nanogold particle comprises nanosphere, nanometer rods, nano wire, nanobelt.
In another preference, the content of described propionic acid is 0.25wt%-1.5wt%.
In another preference, the content of described propionic acid is 0.5wt%-1wt%.
In another preference, the adsorption of described nanogold particle has albumen.
In another preference, described albumen is bovine serum albumin(BSA), or oralbumin.
A second aspect of the present invention provides a kind of composition, and described composition comprises:
(a) the described food of first aspect;
(b) active component, wherein,
Described active constituent loading is on described nanogold particle.
In another preference, described active component is medicine or nucleic acid.
A third aspect of the present invention provides the preparation method of the described food of first aspect, may further comprise the steps:
(a) propionic acid is joined obtain the food first product in the animal food that does not condense;
(b) nano-Au solution is added in the described food first product that step (a) obtains, obtains food claimed in claim 1.
In another preference, mixing temperature is 50-55 ℃ in the described step (b).
In another preference, in the front step that adopts albumen that nanogold particle is processed that also comprises of described step (b):
(b0) adopt the protein solution of 0.1-100 μ g/ml to process described nanogold particle 5-30min, make the adsorption albumen of nanogold particle.
In another preference, described albumen is bovine serum albumin(BSA), or oralbumin.
In another preference, described protein concentration is 0.1-25 μ g/ml, preferably, is 2-10 μ g/ml.
In another preference, described animal food is the food of fruit bat food, C. Elegans Automatic Screening food, rabbit, the food of dog, food, mouse food or the rat food of pig.
A fourth aspect of the present invention provides the purposes of the described food of first aspect, is selected from lower group:
(a) be used for letting animals feed;
(b) for the fat content that improves animal;
(c) for the preparation of the composition of carrying medicament or nucleic acid.
Food of the present invention, nanogold particle is evenly dispersed in the food, can stably be absorbed by animal tissue, change the fat metabolism, improve the activity of Akt signal path, can be used for letting animals feed, improve the fat content of animal, also can be for the preparation of the composition of carrying medicament or nucleic acid, for further research nm of gold carrying medicament or nucleic acid in animal body the research of biological effect lay the foundation.
In should be understood that within the scope of the present invention, above-mentioned each technical characterictic of the present invention and can making up mutually between specifically described each technical characterictic in below (eg embodiment), thus consist of new or preferred technical scheme.As space is limited, this tired stating no longer one by one.
The specific embodiment
The present inventor is through extensive and deep research, and unexpected discovery in the presence of propionic acid, mixes nanogold particle with animal food, and nanogold particle can be evenly dispersed in the food, is difficult for assembling.On this basis, finished the present invention.
Term
Nanogold particle (also claiming nm of gold)
Nanogold particle refers to the gold particle of size in the 1-100nm scope, has high electron density, dielectric property and catalytic action, can be combined with multiple large biological molecule, and not affect its biologically active.
The preparation method of nanogold particle mainly adopts reducing agent, such as natrium citricum, sodium borohydride etc., and the reduction gold chloride.By control reaction condition, reagent dosage, can obtain the good gold grain of monodispersity in the preparation process.Nanogold particle used herein can adopt that any known method prepares in the prior art.
Nanogold particle and-SH ,-NH
2,-the groups such as CH have very strong affinity.These character make gold grain that special biochemical, and the application of medical science aspect arranged.Gold grain links to each other by covalent bond with the oligonucleotides of alkyl modified, forms nanometer bio probe, can be used for the detection of dna sequence dna; Nm of gold carrying antisense DNA or s iRNA are combined with the mRNA of target gene after entering cell, can suppress the expression of target gene.Gold grain can also link to each other with protein, as linking to each other with specific antibodies, detects its corresponding antigen.In vivo, the gold grain that carries medicine and specific antibodies (such as the antibody of cancer cell surface antigen) can enter target cell specifically, by growth, metabolism and the differentiation etc. of the drug influence target cell of carrying.Use gold grain to carry out DNA or protein detection as probe, not only simple to operate, phenomenon obviously but also the degree of accuracy, highly sensitive; Carry nucleic acid or medicine take gold grain as carrier and have high efficiency and specificity.In addition, gold grain itself is also influential to cell activities.Gold grain can change its Acidity of Aikalinity after entering lysosome, thereby affects cell autophagy.
Universal method
Nanogold particle is assembled the detection of situation:
Nano-Au solution is red, and color transition is blue after occuring to assemble.Impact because of the yeast color after food mixes with the nm of gold monomer is rufous, changes such as color, can think and assemble.
If color does not change, will comprise the food of nanogold particle after room temperature is placed 5 days, whether the nanogold particle that adopts transmission electron microscope to detect in the food a small amount of gathering occurs.The nm of gold clustering phenomena did not occur after 5 days in preservation under the room temperature, can think that the food that comprises nanogold particle is stable.
Food (food)
The invention provides a kind of novel food, contain nanogold particle and 0.1wt%-3wt% propionic acid in the described food, by the gross weight of food.
In another preference, described propionic acid is both as anticorrisive agent, also as the agglutination inhibitor that suppresses the nanogold particle gathering.
In another preference, described food has following one or more characteristics:
(a) stability: the nm of gold clustering phenomena did not occur after 5 days in preservation under the room temperature;
(b) anti-corrosive properties: preservation food spoilage do not occur after 5 days under the room temperature.
In another preference, the concentration of described nanogold particle is 0.1-10nM.
In another preference, the concentration of described nanogold particle is 0.5-5nM, preferably, is 0.5-2nM.
In another preference, by the gross weight of food, the concentration of described nanogold particle is 2.4-240 μ g/ml, preferably, is 12-192 μ g/ml.
In another preference, by the gross weight of food, the concentration of described nanogold particle is 12-120 μ g/ml, preferably, is 12-48 μ g/ml.
The average grain diameter of described nanogold particle is 1-100nm.
Preferably, the average grain diameter of described nanogold particle is 5-60nm, and more preferably, the average grain diameter of described nanogold particle is 10-40nm.
In another preference, described nanogold particle is evenly distributed in the described food.
In another preference, described nanogold particle comprises nanosphere, nanometer rods, nano wire, nanobelt.
In another preference, the content of described propionic acid is 0.25wt%-1.5wt%.
In another preference, the content of described propionic acid is 0.5wt%-1wt%.
In another preference, the adsorption of described nanogold particle has albumen.
In another preference, described albumen is bovine serum albumin(BSA), or oralbumin.
In another preference, food of the present invention comprises animal food, nanogold particle and 0.1wt%-3wt% propionic acid, by the gross weight of food.
Animal self can not be made organic matter, must obtain food from the external world, to obtain growth desired nutritional and energy.As used herein, term " animal food ", " animal foodstuff " or " animal feed " have identical implication, all refer to the food of letting animals feed.As in laboratory research, raise the food of model animal pig, dog, rabbit, mouse, rat etc.The prescription of this food can adopt food preparation known in the art.
Prescription and compound method such as fruit bat food are as follows:
The 10wt% dusty yeast, 10wt% sucrose, the 1.5wt% agar powder, anticorrisive agent (0.1wt% Sodium Benzoate, or 0.025wt% methyl hydroxybenzoate, or 0.7wt% propionic acid) surplus is water.
Dusty yeast, sucrose, agar powder are added to the water, and are cooled to 60-65 ℃ after boiling, and add anticorrisive agent.
Wherein, propionic acid is the carboxylic acid of three carbon, and chemical formula is CH
3CH
2COOH.Pure propionic acid is colourless, corrosive liquid, with penetrating odor, can be used as esterifying agent, solvent, plasticizer, emulsifying agent, herbicide, can also be used for preparation food, as anticorrisive agent.
Prescription and the compound method of C. Elegans Automatic Screening food are as follows:
1.7wt% agar, 0.3wt%NaCl, the 0.25wt% peptone, the 5mg/L cholesterol, 1mM calcium chloride, 1mM magnesium sulfate, the 25mM potassium phosphate, surplus is water.
Agar, NaCl, peptone, cholesterol is added to the water, and is cooled to 60-65 ℃ after boiling, and adds calcium chloride, magnesium sulfate, potassium phosphate, then cooling is condensed.Smear Escherichia coli before the use.
Mouse feed prescription: corn flour 27wt%, wheat bran 19wt%, rice 16wt%, soya-bean cake 16wt%, fish meal 13wt%, calcium powder 3wt%, bone meal 3wt%, dusty yeast 2.3wt%, salt 0.5wt%, B B-complex 0.1wt%, micro-0.1wt%.
Composition
Composition of the present invention comprises aforementioned food and active component, and wherein said active constituent loading is on described nanogold particle.
Composition of the present invention is food compositions or composite medicine.
As used herein, term " active component " is medicine or nucleic acid, can adopt the known method of prior art to load on the nanogold particle.
Composition of the present invention enters in the animal body by oral route.
The preparation method
The preparation method of food of the present invention may further comprise the steps:
(a) propionic acid is joined obtain the food first product in the animal food that does not condense;
(b) nano-Au solution is added in the described food first product that step (a) obtains, obtains food claimed in claim 1.
In another preference, in the described step (b), nano-Au solution is added in the described food first product that step (a) obtains, the final concentration of described nm of gold is 0.1-10nM.Preferably be 0.5-5nM, more preferably, be 0.5-2nM.
In another preference, mixing temperature is 50-55 ℃ in the described step (b).
In another preference, the front step that adopts albumen that nanogold particle is processed that also comprises of described step (b):
(b0) adopt the protein solution of 0.1-100 μ g/ml to process described nanogold particle 5-30min, make the adsorption albumen of nanogold particle.
In another preference, described albumen is bovine serum albumin(BSA), or oralbumin.
In another preference, described protein concentration is 0.1-25 μ g/ml, preferably, is 2-10 μ g/ml.
Described animal food is the food of fruit bat food, C. Elegans Automatic Screening food, rabbit, the food of dog, food, mouse food or the rat food of pig.Can adopt the whole bag of tricks preparation known in the art.
Purposes
Food of the present invention can be used for letting animals feed.
In addition, food of the present invention also can be used for improving the fat content of animal.
Further, food of the present invention can be for the preparation of the composition of carrying medicament or nucleic acid.
In another preference, described animal is fruit bat, Samsonite nematode, mouse, rat.
The above-mentioned feature that the present invention mentions, or the feature that embodiment mentions can any combination.All features that this case specification discloses can with any composition forms and usefulness, each feature that discloses in the specification can be replaced by any alternative characteristics of identical, impartial or similar purpose that provides.Therefore except special instruction is arranged, the feature that discloses only is the general example of equalization or similar features.
Major advantage of the present invention is:
(1) provides a kind of novel animal food.
(2) animal food provided by the invention, nm of gold is dispersed in the animal food, and concentration all is difficult for assembling with, nanogold particle everywhere.
(3) the invention provides a kind of preparation method of food of nano-golden particle-containing.
(4) lay the foundation for nano material or medicine carrying/nucleic acid nano material research in vivo.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example is usually according to normal condition or the condition of advising according to manufacturer.
Unless otherwise defined, employed all specialties are identical with the meaning that scientific words and one skilled in the art are familiar with in the literary composition.In addition, any method similar or impartial to described content and material all can be applicable in the inventive method.The usefulness that better implementation method described in the literary composition and material only present a demonstration.
Embodiment 1
The preparation of nano-Au solution
The gold chloride reducing process of employing standard namely adds gold chloride in boiling water, add rapidly natrium citricum under the condition of vigorous stirring, forms the nano-Au solution mother liquor of 20nM after the cooling.
Embodiment 2
The preliminary treatment of nanogold particle
2a: be 1: 200 according to volume ratio, 100 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in the nano-Au solution of embodiment 1 preparation, the final concentration that makes bovine serum albumin(BSA) is the 0.5 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min makes nm of gold adsorption BSA.
2b: be 1: 200 according to volume ratio, 200 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in the nano-Au solution of embodiment 1 preparation, the final concentration that makes bovine serum albumin(BSA) is the 1 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min makes nm of gold adsorption BSA.
2c: be 1: 200 according to volume ratio, 400 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in the nano-Au solution of embodiment 1 preparation, the final concentration that makes bovine serum albumin(BSA) is the 2 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min makes nm of gold adsorption BSA.
2d: be 1: 200 according to volume ratio, 600 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in the nano-Au solution of embodiment 1 preparation, the final concentration that makes bovine serum albumin(BSA) is the 3 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min makes nm of gold adsorption BSA.
2e: be 1: 200 according to volume ratio, 1000 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in the nano-Au solution of embodiment 1 preparation, the final concentration that makes bovine serum albumin(BSA) is the 5 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min makes nm of gold adsorption BSA.
2f: be 1: 200 according to volume ratio, 1400 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution are joined in the nano-Au solution of embodiment 1 preparation, the final concentration that makes bovine serum albumin(BSA) is the 7 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min makes nm of gold adsorption BSA.
2g: be 1: 200 according to volume ratio, with 2000 μ g/ml bovine serum albumin(BSA) (BSA) aqueous solution with 1: 200 dilution proportion in the nano-Au solution of embodiment 1 preparation, the final concentration that makes bovine serum albumin(BSA) is the 10 μ g/ml aqueous solution, the concentration of nanogold particle is substantially constant, be about 20nM, parcel 10min makes nm of gold adsorption BSA.
Embodiment 3
The preparation of food
Preparation fruit bat food, being cooled to 50-55 ℃ adds respectively in proportion through embodiment 2a～nm of gold of the adsorption BSA that 2g processing obtains, the nano-Au solution that embodiment 1 obtains, mix, the final concentration of nanogold particle in food is 2nM, the content of anticorrisive agent propionic acid is 0.7wt%, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
Preparation fruit bat food, being cooled to 48 ℃ adds respectively in proportion through embodiment 2a～nm of gold of the adsorption BSA that 2g processing obtains, the nano-Au solution that embodiment 1 obtains, mix, the final concentration of nanogold particle in food is 2nM, the content of anticorrisive agent propionic acid is 0.7wt%, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
Preparation fruit bat food, being cooled to 60 ℃ adds respectively in proportion through embodiment 2a～nm of gold of the adsorption BSA that 2g processing obtains, the nano-Au solution that embodiment 1 obtains, mix, the final concentration of nanogold particle in food is 2nM, the content of anticorrisive agent propionic acid is 0.7wt%, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
Table 1 temperature and BSA are on the impact of stability of nano gold
The result of table 1 shows, concentration 〉=0.5 μ g/ml BSA after especially among concentration 〉=2 μ g/ml BSA nm of gold being wrapped up, has facilitation to the stability of nm of gold in food.Between 50-55 ℃, nm of gold is mixed with the fruit bat food that does not condense, can prevent the gathering of nm of gold.Mix nm of gold and fruit bat food under 55 ℃ the temperature being higher than, nm of gold is assembled easily.Be lower than under 50 ℃ the temperature, fruit bat food condenses rapidly, and nm of gold is not easy to mix with food.
Embodiment 4
The preparation of food
4a: at 60 ℃ propionic acid is joined in the fruit bat food, be cooled to 50-55 ℃ and add in proportion the nm of gold of processing the adsorption BSA that obtains through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, and the content of propionic acid is 0.7wt%.
4b: methyl hydroxybenzoate is joined in the fruit bat food at 60 ℃, be cooled to 50-55 ℃ and add in proportion the nm of gold of processing the adsorption BSA that obtains through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, and the content of methyl hydroxybenzoate is 0.025wt%.
4c: Sodium Benzoate is joined in the fruit bat food at 60 ℃, be cooled to 50-55 ℃ and add in proportion the nm of gold of processing the adsorption BSA that obtains through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, and the content of Sodium Benzoate is 0.1wt%.
4d: propionic acid and methyl hydroxybenzoate are joined in the fruit bat food at 60 ℃, be cooled to 50-55 ℃ and add in proportion the nm of gold of processing the adsorption BSA that obtains through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, the content of propionic acid is 0.7wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
4e: propionic acid and Sodium Benzoate are joined in the fruit bat food at 60 ℃, be cooled to 50-55 ℃ and add in proportion the nm of gold of processing the adsorption BSA that obtains through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, the content of propionic acid is 0.7wt%, and the content of Sodium Benzoate is 0.1wt%.
4f: Sodium Benzoate and methyl hydroxybenzoate are joined in the fruit bat food at 60 ℃, be cooled to 50-55 ℃ and add in proportion the nm of gold of processing the adsorption BSA that obtains through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
4g: propionic acid, methyl hydroxybenzoate and Sodium Benzoate are joined in the fruit bat food at 60 ℃, be cooled to 50-55 ℃ and add in proportion the nm of gold of processing the adsorption BSA that obtains through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM, the content of propionic acid is 0.7wt%, the content of Sodium Benzoate is 0.1wt%, and the content of methyl hydroxybenzoate is 0.025wt%.
4h: do not add anticorrisive agent, fruit bat food is cooled to 50-55 ℃ adds in proportion the nm of gold of processing the adsorption BSA that obtains through embodiment 2c, mix, the final concentration of nanogold particle in food is 2nM.
Table 2 anticorrisive agent is on the impact of stability of nano gold
| Embodiment | 4a | 4b | 4c | 4d | 4e | 4f | 4g | 4h |
| Sodium Benzoate | - | - | + | - | + | + | + | - |
| Methyl hydroxybenzoate | - | + | - | + | - | + | + | - |
| Propionic acid | + | - | - | + | + | - | + | - |
| Whether assemble after 2 days | No | Be | Be | No | No | Be | Be | No |
| Whether assemble after 5 days | No | Be | Be | Be | Be | Be | Be | Be |
Wherein ,+expression is added, and-expression is not added
Table 2 is the result show, Sodium Benzoate and methyl hydroxybenzoate can be promoted the gathering of nm of gold in food.When only adding propionic acid, the food that comprises nm of gold was still kept color after 5 days constant, detects nanogold particle through transmission electron microscope and do not assemble.What is interesting is that even do not add any anticorrisive agent, nm of gold still can be assembled after 5 days, illustrate that propionic acid brought into play the function outside the anticorrisive agent, prevent that namely nm of gold from assembling in food.
Embodiment 5
The preparation of food
5a: basic identical with the method for embodiment 4a, difference only is that the final concentration of nanogold particle in food is 0.5nM.
5b: basic identical with the method for embodiment 4a, difference only is that the final concentration of nanogold particle in food is 1nM.
5c: basic identical with the method for embodiment 4a, difference only is that the final concentration of nanogold particle in food is 3nM.
5d: basic identical with the method for embodiment 4a, difference only is that the final concentration of nanogold particle in food is 5nM.
5e: basic identical with the method for embodiment 4a, difference only is that the content of propionic acid is 0.5wt%.
5f: basic identical with the method for embodiment 4a, difference only is that the content of propionic acid is 1wt%.
5g: basic identical with the method for embodiment 4a, difference only is that the content of propionic acid is 1.5wt%.
5h: basic identical with the method for embodiment 4a, difference only is, adds the nm of gold of processing the adsorption BSA that obtains through embodiment 2e.
5i: basic identical with the method for embodiment 4a, difference only is, adds the nm of gold of processing the adsorption BSA that obtains through embodiment 2g.
5j: basic identical with the method for embodiment 4a, difference only is, propionic acid is joined in the food of C. Elegans Automatic Screening.
The result shows that in the food of embodiment 5a-5j preparation, nm of gold is assembled.
Embodiment 6
Take in research in the body
Adopt the food of embodiment 4a preparation to feed fruit bat, namely allow drosophila larvae grow at food.After 4 days, separate the organ (epidermis, intestines, fat-body, imaginal discs, brain and tracheae) of fruit bat, adopt the picked-up situation of I transmission electron microscope observing or detection nm of gold in individual organ.
The result shows that nm of gold can be taken in by fruit bat and absorb, and mainly is distributed in epidermis, intestines and fat-body, and it is less to distribute in imaginal discs, brain and tracheae.
Embodiment 7
Internal metabolism research
Adopt the food of embodiment 4a preparation to feed fruit bat, namely allow drosophila larvae grow at food.
Take the food of plus nano gold not as control group.
After 4 days, measure fat content with surveying fat kit (Sigma) after the cracking of employing fruit bat.
Compare with the contrast fruit bat, its fat content rises, and detects the Akt content that activates through Western Blot, finds to rise with the activity of the closely-related Akt signal path of fruit bat fat metabolism, illustrates after nm of gold enters the fruit bat tissue to change the fat metabolism.
Above result shows, the food that comprises nm of gold through the inventive method preparation not only has stable physical property, does not assemble, the more important thing is and effectively nm of gold to be imported in the fruit bat body that the research in the fruit bat body is laid a good foundation for nm of gold carrier band medicine or nucleic acid molecules.
All quote in this application as a reference at all documents that the present invention mentions, just as each piece document is quoted separately as a reference.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after having read above-mentioned instruction content of the present invention, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (10)
1. a food is characterized in that, contains nanogold particle and 0.1wt%-3wt% propionic acid in the described food, by the gross weight of food.
2. food as claimed in claim 1 is characterized in that, the concentration of described nanogold particle is 0.1-10nM.
3. food as claimed in claim 1 is characterized in that, the content of described propionic acid is 0.25wt%-1.5wt%.
4. food as claimed in claim 1 is characterized in that, the adsorption of described nanogold particle has albumen.
5. a composition is characterized in that, described composition comprises:
(a) food claimed in claim 1;
(b) active component, wherein,
Described active constituent loading is on described nanogold particle.
6. composition as claimed in claim 5 is characterized in that, described active component is medicine or nucleic acid.
7. the preparation method of food as claimed in claim 1 is characterized in that, said method comprising the steps of:
(a) propionic acid is joined obtain the food first product in the animal food that does not condense;
(b) nano-Au solution is added in the described food first product that step (a) obtains, obtains food claimed in claim 1.
8. preparation method as claimed in claim 7 is characterized in that, in the front step that adopts albumen that nanogold particle is processed that also comprises of described step (b):
(b0) adopt the protein solution of 0.1-100 μ g/ml to process described nanogold particle 5-30min, make the adsorption albumen of nanogold particle.
9. preparation method as claimed in claim 7 is characterized in that, described animal food is the food of fruit bat food, C. Elegans Automatic Screening food, rabbit, the food of dog, food, mouse food or the rat food of pig.
10. the purposes of food as claimed in claim 1 is characterized in that, described purposes is selected from lower group:
(a) be used for letting animals feed;
(b) for the fat content that improves animal;
(c) for the preparation of the composition of carrying medicament or nucleic acid.
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Address after: 200031 Yueyang Road, Shanghai, No. 319, No. Patentee after: Shanghai Institute of nutrition and health, Chinese Academy of Sciences Address before: 200031 Yueyang Road, Shanghai, No. 319, No. Patentee before: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES |
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