CN101144068B - Fast dissolution preparation method for blood culture medium - Google Patents
Fast dissolution preparation method for blood culture medium Download PDFInfo
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- CN101144068B CN101144068B CN 200710045544 CN200710045544A CN101144068B CN 101144068 B CN101144068 B CN 101144068B CN 200710045544 CN200710045544 CN 200710045544 CN 200710045544 A CN200710045544 A CN 200710045544A CN 101144068 B CN101144068 B CN 101144068B
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Abstract
The present invention relates to the technology field of the biomedicine, in particular to a preparation method for the rapid dissolving of a blood culture medium. Firstly, the raw materials of the culture medium are weighed, and the raw materials include that peptone is 10 g, beef extract is 3 g, sodium chloride is 5 g, sodium citrate is 3 g, and agar is 0.5 g; the fast solution is prepared by the preparation method that 24.6 percent of magnesium sulfate solution is prepared to be used as fast solution 1 to be reserved; 0.5 percent of paraaminobenzoic acid solution is prepared to be used as fast solution 2 to be reserved; the mixing is realized by that about 20 ml raw material of the fast solution is taken and 10 ml fast solution is taken to be added into the raw material of the culture medium and agitated into paste, and then 1000ml distilled water is added into to be rocked and mixed, and statically positioned for three to five minutes to obtain the transparent culture medium fluid; the pH value is adjusted to be 7.4; the culture medium fluid is subpackaged in a sealed liquid bottle, and then sterilized in the high pressure; the finished product of the blood culture medium includes the obtaining process that 100 units of penicillase are added in each bottle through an injection syringe or not added, after the sterility test, the blood culture medium is reserved in the room temperature. Compared with the prior art, the present invention eliminates the long time heating dissolving and filter process, saves the preparation time and the filter material, and the operation issimple and convenient.
Description
[technical field]
The present invention relates to field of biomedicine technology, specifically a kind of fast dissolution preparation method for blood culture medium.
[background technology]
Blood culture medium is prepared cumbersome according to a conventional method, and the steps, particularly heating for dissolving such as weighing, Jia Shui, heating for dissolving, filtration, accent pH value, packing, autoclaving are arranged usually, length consuming time, weak effect has muddiness during substratum, impact is to the adjusting of pH value and the observation of bacterial growth situation.
[summary of the invention]
Purpose of the present invention overcomes the deficiencies in the prior art exactly, and the instant compounding process of a kind of blood culture medium that provides.
For achieving the above object, design a kind of fast dissolution preparation method for blood culture medium,
(1) take the culture medium raw material of following component, form instant liquid: peptone 10g, extractum carnis 3g, sodium-chlor 5g, Sodium Citrate 3g, nucleic acid 2g, agar 0.5g;
(2) configure instant liquid: the Adlerika of preparation 24.6% is standby as instant liquid 1; The para-amino benzoic acid solution of preparation 0.5% is standby as instant liquid 2;
(3) mix: get the described instant liquid 1 of 20ml and the instant liquid 2 of 10ml, add in culture medium raw material, stir into pasty state, add the distilled water of 1000ml and shake mixing, obtained Clear ﹠ Transparent liquid medium in standing 3-5 minute;
(4) transfer pH value: the pH value of liquid medium is adjusted to 7.4;
(5) packing is loaded on the liquid medium that the mixes up pH value amount with every bottle of 50ml in the sealing fluid bottle;
(6) autoclaving;
(7) get the blood culture medium finished product; Add penicillinase 100 units or do not add with every bottle of syringe, after sterility test, room storage is standby.
Described instant liquid 1 is that 24.6g sal epsom adding distil water 100ml dissolving is made.
Described instant liquid 2 is that 0.5g para-amino benzoic acid adding distil water 100ml dissolving is made.
Described accent pH value is to adopt ionocolorimeter to transfer the pH value of substratum.
Described autoclaving was in high-pressure sterilizing pot, with the lasting sterilization of the pressure of 0.7-1 kilogram 15 minutes.
The blood culture medium of described method preparation can be applicable to clinical bacteriology check and blood, marrow is cultivated and checked.
The present invention has compared with prior art removed long-time heating dissolving and filtration step, saves the preparation time, and has saved filtering material, makes easy and simple to handlely, is beneficial to batch production.
[description of drawings]
Fig. 1 is process flow sheet of the present invention.
Appointment Fig. 1 is Figure of abstract.
Referring to Fig. 1,1 for taking culture medium raw material; 2 is preparation of instant liquid; 3 for mixing; 4 for transferring pH value; 5 are packing; 6 is autoclaving; 7 be the blood culture medium finished product.
[specific embodiment]
The present invention is further illustrated below in conjunction with accompanying drawing, and the present invention is still more clearly concerning the people of this professional skill field.
Embodiment:
Take culture medium raw material: peptone 10g, extractum carnis 3g, sodium-chlor 5g, Sodium Citrate 3 grams, nucleic acid 2 grams, agar 0.5 gram, standby.
Preparation of instant liquid:
Instant liquid 1: it is standby that the sal epsom that takes 24.6g adds the dissolving of 100ml distilled water to make 24.6% Adlerika;
Instant liquid 2: the para-amino benzoic acid adding distil water 100ml that takes 0.5g makes 0.5% para-amino benzoic acid solution for standby;
Mix: culture medium raw material is put into large Erlenmeyer flask, then get the instant liquid 1 of 20ml and the instant liquid 2 of 10ml adds in culture medium raw material, after stirring into pasty state, add again the distilled water of 1000ml and shake mixing, standing 3-5 minute, just obtain Clear ﹠ Transparent liquid medium, dissolution rate is fast;
Transfer pH value: with ionocolorimeter, the pH value of liquid medium is adjusted to 7.4;
Packing: being 7.4 liquid medium with pH value is loaded in liquid bottles with the amount of every bottle of 50ml, and with soft rubber ball envelope bottleneck, soft rubber ball is outward again with the aluminium lid press seal;
Autoclaving: in high-pressure sterilizing pot, with the lasting sterilization of the pressure of 0.7-1 kilogram 15 minutes.
At last, add penicillinase 100 units with every bottle of syringe, play the antibiotic effect of neutralization, also can not add, after sterility test, room storage is standby, and these blood culture mediums namely can be used for the use of blood, bone marrow fluid microbial culture.
Claims (5)
1. fast dissolution preparation method for blood culture medium, it is characterized in that: (1) takes the culture medium raw material of following component: peptone 10g, extractum carnis 3g, sodium-chlor 5g, Sodium Citrate 3g, nucleic acid 2g, agar 0.5g; (2) preparation of instant liquid: the Adlerika of preparation 24.6% is standby as instant liquid 1; The para-amino benzoic acid solution of preparation 0.5% is standby as instant liquid 2; (3) mix: above-mentioned instant liquid 1 is got 20ml and above-mentioned instant liquid 2 get 10ml and add in culture medium raw material, stir into pasty state, add the distilled water of 1000ml and shake mixing, obtained Clear ﹠ Transparent liquid medium in standing 3-5 minute; (4) transfer pH value: the pH value of liquid medium is adjusted to 7.4; (5) packing is loaded on the liquid medium that the mixes up pH value amount with every bottle of 50ml in the sealing fluid bottle; (6) autoclaving; (7) get the blood culture medium finished product: add penicillinase 100 units or do not add with every bottle of syringe, after sterility test, room storage is standby.
2. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described instant liquid 1 is that 24.6g sal epsom adding distil water 100ml dissolving is made.
3. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described instant liquid 2 is that 0.5g para-amino benzoic acid adding distil water 100ml dissolving is made.
4. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described accent pH value is to adopt ionocolorimeter to transfer the pH value of substratum.
5. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described autoclaving is in high-pressure sterilizing pot, continues sterilization 15 minutes with the pressure of 0.7-1 kilogram.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710045544 CN101144068B (en) | 2007-09-03 | 2007-09-03 | Fast dissolution preparation method for blood culture medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710045544 CN101144068B (en) | 2007-09-03 | 2007-09-03 | Fast dissolution preparation method for blood culture medium |
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| CN101144068A CN101144068A (en) | 2008-03-19 |
| CN101144068B true CN101144068B (en) | 2013-06-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200710045544 Active CN101144068B (en) | 2007-09-03 | 2007-09-03 | Fast dissolution preparation method for blood culture medium |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337184B (en) * | 2011-06-09 | 2012-11-07 | 王惠萱 | Solvent, preparation method and application thereof |
| CN102747132A (en) * | 2012-07-23 | 2012-10-24 | 史煜波 | Aerobic bacterial culture solution for machines |
| CN106222236A (en) * | 2016-07-27 | 2016-12-14 | 郑州点石生物技术有限公司 | Microorganism detection reagent and preparation method thereof in blood |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125619A (en) * | 1994-12-30 | 1996-07-03 | 江苏省卫生防疫站 | Preparation method of bacteriophagic leech and vibrio ecological preparation |
| CN1181890A (en) * | 1996-11-08 | 1998-05-20 | 包头粮油食品科学研究所 | Biological bran converting method utilizing photosynthetic bacteria and its application |
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2007
- 2007-09-03 CN CN 200710045544 patent/CN101144068B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125619A (en) * | 1994-12-30 | 1996-07-03 | 江苏省卫生防疫站 | Preparation method of bacteriophagic leech and vibrio ecological preparation |
| CN1181890A (en) * | 1996-11-08 | 1998-05-20 | 包头粮油食品科学研究所 | Biological bran converting method utilizing photosynthetic bacteria and its application |
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| CN101144068A (en) | 2008-03-19 |
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Effective date of registration: 20221228 Address after: 650000 floor 2, building 4, pharmaceutical technology R & D base, intersection of Haiyuan North Road and Keji Road (Yunnan Haobang Investment Co., Ltd.), Kunming, Yunnan Patentee after: Yunnan Dean Medical Laboratory Co.,Ltd. Address before: 650032 Department of clinical laboratory, Kunming general hospital, No. 212 military area, Daguan Road, Yunnan, Kunming Patentee before: Wang Huixuan |