CN101144068A - Fast dissolution preparation method for blood culture medium - Google Patents
Fast dissolution preparation method for blood culture medium Download PDFInfo
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- CN101144068A CN101144068A CNA200710045544XA CN200710045544A CN101144068A CN 101144068 A CN101144068 A CN 101144068A CN A200710045544X A CNA200710045544X A CN A200710045544XA CN 200710045544 A CN200710045544 A CN 200710045544A CN 101144068 A CN101144068 A CN 101144068A
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Abstract
The present invention relates to the technology field of the biomedicine, in particular to a preparation method for the rapid dissolving of a blood culture medium. Firstly, the raw materials of the culture medium are weighed, and the raw materials include that peptone is 10 g, beef extract is 3 g, sodium chloride is 5 g, sodium citrate is 3 g, and agar is 0.5 g; the fast solution is prepared by the preparation method that 24.6 percent of magnesium sulfate solution is prepared to be used as fast solution 1 to be reserved; 0.5 percent of paraaminobenzoic acid solution is prepared to be used as fast solution 2 to be reserved; the mixing is realized by that about 20 ml raw material of the fast solution is taken and 10 ml fast solution is taken to be added into the raw material of the culture medium and agitated into paste, and then 1000ml distilled water is added into to be rocked and mixed, and statically positioned for three to five minutes to obtain the transparent culture medium fluid; the pH value is adjusted to be 7.4; the culture medium fluid is subpackaged in a sealed liquid bottle, and then sterilized in the high pressure; the finished product of the blood culture medium includes the obtaining process that 100 units of penicillase are added in each bottle through an injection syringe or not added, after the sterility test, the blood culture medium is reserved in the room temperature. Compared with the prior art, the present invention eliminates the long time heating dissolving and filter process, saves the preparation time and the filter material, and the operation is simple and convenient.
Description
[technical field]
The present invention relates to field of biomedicine technology, a kind of specifically fast dissolution preparation method for blood culture medium.
[background technology]
Blood culture medium is prepared cumbersome according to a conventional method, and steps, particularly heating for dissolving such as weighing, Jia Shui, heating for dissolving, filtration, accent pH value, packing, autoclaving are arranged usually, length consuming time, weak effect has muddiness during substratum, influence is to the adjusting of pH value and the observation of bacterial growth situation.
[summary of the invention]
Purpose of the present invention overcomes the deficiencies in the prior art exactly, and the instant compounding process of a kind of blood culture medium that provides.
For achieving the above object, design a kind of fast dissolution preparation method for blood culture medium, it is characterized in that: (1) takes by weighing the culture medium raw material of following component and forms instant liquid: peptone 10g, extractum carnis 3g, sodium-chlor 5g, Sodium Citrate 3g, nucleic acid 2g, agar 0.5g; (2) preparation of instant liquid: preparation 24.6% Adlerika is standby as instant liquid 1; Preparation 0.5% para-amino benzoic acid solution is standby as instant liquid 2; (3) mix: will the raw material of above-mentioned instant liquid get 20ml and above-mentioned preparation of instant liquid and get 10ml and add in the culture medium raw material, stir into pasty state, the distilled water of adding 1000ml also shakes mixing, leaves standstill and obtains Clear ﹠ Transparent liquid medium in 3-5 minute; (4) transfer pH value: the pH value of liquid medium is adjusted to 7.4; (5) packing is loaded on the liquid medium that the mixes up pH value amount with every bottle of 50ml in the sealing fluid bottle; (6) autoclaving; (7) the blood culture medium finished product: add penicillinase 100 units or do not add with every bottle of syringe, after the sterility test, room storage is standby.Described instant liquid is that 24.6g sal epsom adding distil water 100ml dissolving is made.Described preparation of instant liquid is that 0.5g para-amino benzoic acid adding distil water 100ml dissolving is made.Described accent pH value is to adopt ionocolorimeter to transfer the pH value of substratum.Described autoclaving was in high-pressure sterilizing pot, with the lasting sterilization of the pressure of 0.7-1 kilogram 15 minutes.The blood culture medium of described method preparation can be applicable to clinical bacteriology check and blood, marrow is cultivated and checked.
The present invention has compared with prior art removed long-time heating dissolving and filtration step, saves the preparation time, and has saved filtering material, makes easy and simple to handlely, is beneficial to batch process.
[description of drawings]
Fig. 1 is a process flow sheet of the present invention.
Appointment Fig. 1 is a Figure of abstract.
Referring to Fig. 1,1 for taking by weighing culture medium raw material; 2 is preparation of instant liquid; 3 for mixing; 4 for transferring pH value; 5 are packing; 6 is autoclaving; 7 be the blood culture medium finished product.
[specific embodiment]
The present invention is further illustrated below in conjunction with accompanying drawing, and the present invention is still more clearly concerning the people of this professional skill field.
Embodiment:
Take by weighing culture medium raw material: peptone 10g, extractum carnis 3g, sodium-chlor 5 g, Sodium Citrate 3 grams, nucleic acid 2 grams, agar 0.5 gram, standby.
Preparation of instant liquid:
Instant liquid 1: the sal epsom that takes by weighing 24.6g adds the 100ml dissolved in distilled water, and to make 24.6% Adlerika standby;
Instant liquid 2: the para-amino benzoic acid adding distil water 100ml that takes by weighing 0.5g makes 0.5% para-amino benzoic acid solution for standby;
Mix: culture medium raw material is put into big Erlenmeyer flask, gets the instant liquid 2 of instant liquid 1 of 20ml and 10ml then and add in the culture medium raw materials, stir into pasty state after, add the distilled water of 1000ml again and shake mixing, left standstill 3-5 minute, and just obtained Clear ﹠ Transparent liquid medium, dissolution rate is fast;
Transfer pH value: the pH value of liquid medium is adjusted to 7.4 with ionocolorimeter;
Packing: being 7.4 liquid medium with pH value is loaded in the liquid bottles with the amount of every bottle of 50ml, and with soft rubber ball envelope bottleneck, soft rubber ball is outward again with the aluminium lid press seal;
Autoclaving: in high-pressure sterilizing pot, with the lasting sterilization of the pressure of 0.7-1 kilogram 15 minutes.
At last, add penicillinase 100 units with every bottle of syringe, play the antibiotic effect of neutralization, also can not add, after the sterility test, room storage is standby, and these blood culture mediums promptly can be used for the usefulness of blood, bone marrow fluid microbial culture.
Claims (6)
1. fast dissolution preparation method for blood culture medium, it is characterized in that: (1) takes by weighing the culture medium raw material of following component and forms instant liquid: peptone 10g, extractum carnis 3g, sodium-chlor 5g, Sodium Citrate 3g, nucleic acid 2g, agar 0.5g; (2) preparation of instant liquid: preparation 24.6% Adlerika is standby as instant liquid 1; Preparation 0.5% para-amino benzoic acid solution is standby as instant liquid 2; (3) mix: will the raw material of above-mentioned instant liquid get 20ml and above-mentioned preparation of instant liquid and get 10ml and add in the culture medium raw material, stir into pasty state, the distilled water of adding 1000ml also shakes mixing, leaves standstill and obtains Clear ﹠ Transparent liquid medium in 3-5 minute; (4) transfer pH value: the pH value of liquid medium is adjusted to 7.4; (5) packing is loaded on the liquid medium that the mixes up pH value amount with every bottle of 50ml in the sealing fluid bottle; (6) autoclaving; (7) the blood culture medium finished product: add penicillinase 100 units or do not add with every bottle of syringe, after the sterility test, room storage is standby.
2. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described instant liquid is that 24.6g sal epsom adding distil water 100ml dissolving is made.
3. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described preparation of instant liquid is that 0.5g para-amino benzoic acid adding distil water 100ml dissolving is made.
4. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described accent pH value is to adopt ionocolorimeter to transfer the pH value of substratum.
5. a kind of fast dissolution preparation method for blood culture medium as claimed in claim 1 is characterized in that: described autoclaving is in high-pressure sterilizing pot, with the lasting sterilization of the pressure of 0.7-1 kilogram 15 minutes.
6. the application of the described a kind of fast dissolution preparation method for blood culture medium of claim 1 is characterized in that: the blood culture medium of described method preparation can be applicable to clinical bacteriology check and blood, marrow is cultivated and checked.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710045544 CN101144068B (en) | 2007-09-03 | 2007-09-03 | Fast dissolution preparation method for blood culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200710045544 CN101144068B (en) | 2007-09-03 | 2007-09-03 | Fast dissolution preparation method for blood culture medium |
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| CN101144068A true CN101144068A (en) | 2008-03-19 |
| CN101144068B CN101144068B (en) | 2013-06-05 |
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| CN 200710045544 Active CN101144068B (en) | 2007-09-03 | 2007-09-03 | Fast dissolution preparation method for blood culture medium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337184A (en) * | 2011-06-09 | 2012-02-01 | 王惠萱 | Solvent, preparation method and application thereof |
| CN103343157A (en) * | 2012-07-23 | 2013-10-09 | 史煜波 | Bacterial culture solution for detecting pathogenic bacteria in blood |
| CN106222236A (en) * | 2016-07-27 | 2016-12-14 | 郑州点石生物技术有限公司 | Microorganism detection reagent and preparation method thereof in blood |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125619A (en) * | 1994-12-30 | 1996-07-03 | 江苏省卫生防疫站 | Preparation method of bacteriophagic leech and vibrio ecological preparation |
| CN1181890A (en) * | 1996-11-08 | 1998-05-20 | 包头粮油食品科学研究所 | Biological bran converting method utilizing photosynthetic bacteria and its application |
-
2007
- 2007-09-03 CN CN 200710045544 patent/CN101144068B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125619A (en) * | 1994-12-30 | 1996-07-03 | 江苏省卫生防疫站 | Preparation method of bacteriophagic leech and vibrio ecological preparation |
| CN1181890A (en) * | 1996-11-08 | 1998-05-20 | 包头粮油食品科学研究所 | Biological bran converting method utilizing photosynthetic bacteria and its application |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337184A (en) * | 2011-06-09 | 2012-02-01 | 王惠萱 | Solvent, preparation method and application thereof |
| CN102337184B (en) * | 2011-06-09 | 2012-11-07 | 王惠萱 | Solvent, preparation method and application thereof |
| CN103343157A (en) * | 2012-07-23 | 2013-10-09 | 史煜波 | Bacterial culture solution for detecting pathogenic bacteria in blood |
| CN106222236A (en) * | 2016-07-27 | 2016-12-14 | 郑州点石生物技术有限公司 | Microorganism detection reagent and preparation method thereof in blood |
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| Publication number | Publication date |
|---|---|
| CN101144068B (en) | 2013-06-05 |
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Effective date of registration: 20221228 Address after: 650000 floor 2, building 4, pharmaceutical technology R & D base, intersection of Haiyuan North Road and Keji Road (Yunnan Haobang Investment Co., Ltd.), Kunming, Yunnan Patentee after: Yunnan Dean Medical Laboratory Co.,Ltd. Address before: 650032 Department of clinical laboratory, Kunming general hospital, No. 212 military area, Daguan Road, Yunnan, Kunming Patentee before: Wang Huixuan |
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