CN102337184B - Solvent, preparation method and application thereof - Google Patents
Solvent, preparation method and application thereof Download PDFInfo
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- CN102337184B CN102337184B CN2011101556887A CN201110155688A CN102337184B CN 102337184 B CN102337184 B CN 102337184B CN 2011101556887 A CN2011101556887 A CN 2011101556887A CN 201110155688 A CN201110155688 A CN 201110155688A CN 102337184 B CN102337184 B CN 102337184B
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Abstract
The invention discloses a solvent, a preparation method and an application thereof. The solvent provided by the invention has the following effective components of: by weight, 3-7 parts of sodium citrate, 5-9 parts of sodium chloride, 8-12 parts of magnesium sulfate, 2-4 parts of p-aminobenzoic acid, 1-5 parts of 4-Aminoantipyrine, 3-7 parts of disodium hydrogen phosphate and 1-5 parts of potassium dihydrogen phosphate. The solvent provided by the invention can be used to rapidly dissolve protein substances, has low cost and is easy to prepare.
Description
Technical field
The present invention relates to the solvating agent technical field, specifically a kind of solvating agent that is used for solubilising protein class material.
Background technology
Protein substance is infected with on the clothing, or is infected with in fields such as Clinical microorganism checks that last time is difficult to remove on the vessel such as test tube.It is not ideal that solvating agent of the prior art or method are removed protein, and some not too is applicable to the proteinic removal on the vessel in fields such as test, check.Need a kind of solvating agent can remove protein substance fast, up hill and dale for this reason.
In the Clinical microorganism check, need use substratum.Culture medium preparation adopts the mode of heating for dissolving at present.The moisture content of substratum and overflowing of solids component take place easily, thereby the concentration of the substratum that causes processing is inaccurate, influences effect in this process.In addition, adopt the mode of heating to prepare the substratum needs 2 hours, the running time is long, and efficient is low, labor intensive.Based on the problems that type of heating exists, the mode that the inventor has proposed a kind of new employing chemical dissolution prepares the method (CN101144068A) of substratum.The method of the chemical dissolution that CN101144068A proposes prepares substratum and has reduced the running time with respect to heating means, and easy handling.But the substratum among the CN101144068A also exists dissolution rate and stable less-than-ideal problem.
Based on the problem that above-mentioned prior art exists, inventor's active research to be to provide a kind of solvating agent, solubilising protein fast, and the protein that reaches body surface is removed; And can be used for the preparation of substratum, and make medium preparation quick, and stable.
Summary of the invention
In order to solve the problems referred to above that exist in the prior art, the invention provides a kind of solvating agent, fast solubilising protein class material.
In order to solve the problems of the technologies described above, the present invention has adopted following technical scheme:
A kind of solvating agent, its effective ingredient is:
Further, the effective ingredient of said solvating agent is:
The present invention also provides a kind of preparation method of above-mentioned solvating agent, simple, the easy row of method, and it is effective that the solvating agent of preparation is removed protein.Its technical scheme is following:
A kind of preparation method of solvating agent; Comprise the steps: to weigh up each component, be dissolved in 1000 ml distilled waters and get final product by the proportioning of Sodium Citrate 3～7 gram, sodium-chlor 5～9 grams, sal epsom 8～12 grams, para-amino benzoic acid 1～4 gram, 4-aminoantipyrene 1～5 gram, Sodium phosphate, dibasic 3～7 grams, potassium primary phosphate 1～5 gram.
Further, said each component to take by weighing proportioning following: Sodium Citrate 5 grams, sodium-chlor 7 grams, sal epsom 10 grams, para-amino benzoic acid 2 grams, 4-aminoantipyrene 3 grams, Sodium phosphate, dibasic 5 grams, potassium primary phosphate 3 grams.
Another object of the present invention is for providing a kind of substratum, and this medium preparation process is fast, and finished product is stable.The technical scheme that realizes this purpose is following:
Substratum is made by solvating agent dissolved solids raw material, wherein
Solid material comprises peptone 10 mass parts, Carnis Bovis seu Bubali cream 3 mass parts, sodium-chlor 5 mass parts, Sodium Citrate 3 mass parts and agar 0.5 mass parts;
Solvating agent is dissolved in by Sodium Citrate 3～7 mass parts, sodium-chlor 5～9 mass parts, sal epsom 8～12 mass parts, para-amino benzoic acid 1～4 mass parts, 4-aminoantipyrene 1～5 mass parts, Sodium phosphate, dibasic 3～7 mass parts and potassium primary phosphate 1～5 mass parts and makes the solution that concentration is 35 grams per liters in the zero(ppm) water.
Further, said solvating agent makes the solution that concentration is 35 grams per liters for being dissolved in by Sodium Citrate 5 mass parts, sodium-chlor 7 mass parts, sal epsom 10 mass parts, para-amino benzoic acid 2 mass parts, 4-aminoantipyrene 3 mass parts, Sodium phosphate, dibasic 5 mass parts and potassium primary phosphate 3 mass parts in the zero(ppm) water.
The present invention also provides a kind of preparation method of above-mentioned substratum, and its technical scheme is following:
The preparation method of above-mentioned substratum; Comprise the steps: to take by weighing in proportion solid material, and the preparation solvating agent, per 21.5 grams of above-mentioned solid material are mixed into pasty state with 10～20 milliliters of solvating agents; Add zero(ppm) water to 1000 milliliter again; Left standstill 3-5 minute, and regulated pH value to 7.4, promptly get substratum after the sterilization.
Further, per 21.5 grams of said solid material are mixed into pasty state with 15 milliliters of solvating agents.
Compared with prior art, beneficial effect of the present invention is:
1, solvating agent of the present invention can fast and effeciently be removed the protein substance of being infected with on the object.
2, solvating agent cost of the present invention is low, is easy to preparation.
3, the dissolution rate in the preparation process has been accelerated in the application of solvating agent of the present invention in substratum, has further reduced preparation time.
4, the application of solvating agent of the present invention in substratum makes substratum have higher stability.
5, the application of solvating agent of the present invention in substratum makes substratum have higher quality and lower cost.
Embodiment
Below in conjunction with specific embodiment the present invention is described in further detail, but not as to qualification of the present invention.
Embodiment 1
Originally be embodied as the preferred embodiment of solvating agent and preparation method thereof, the effective ingredient of solvating agent is: Sodium Citrate 5 mass parts, sodium-chlor 7 mass parts, sal epsom 10 mass parts, para-amino benzoic acid 2 mass parts, 4-aminoantipyrene 3 mass parts, Sodium phosphate, dibasic 5 mass parts, potassium primary phosphate 3 mass parts.Proportioning by Sodium Citrate 5 grams, sodium-chlor 7 grams, sal epsom 10 grams, para-amino benzoic acid 2 grams, 4-aminoantipyrene 3 grams, Sodium phosphate, dibasic 5 grams and potassium primary phosphate 3 grams during preparation weighs up each component, is dissolved in 1000 ml distilled waters to get final product.
Detect the effect that solvating agent of the present invention is removed protein-based dirt through experiment below.Wherein be made as control group with 84 common on market thimerosals, the solvating agent that the above embodiment of the present invention 1 is prepared is made as experimental group, and cleaning object is test tube, plastics tubing, cotton fabric and desktop.Useless blood after above-mentioned cleaning object used with the hospital routine work is infected with.Remove by the protein on the cleaning objects such as the fabric of blood (rich in proteins) pollution and test tube through the mode of soaking 30 minutes, how many protein residual quantities that detects at last on the cleaning object after cleaning verifies removal effect.Concrete outcome sees the following form 1.
Table 1
| Cleaning object | Control group protein residual quantity | Experimental group protein residual quantity |
| Test tube | 2.7 grams per liter | 0.2 grams per liter |
| Plastics tubing | 2.3 grams per liter | 0.3 grams per liter |
| Cotton fabric | 4.5 grams per liter | 0.7 grams per liter |
| Desktop | 3.8 grams per liter | 0.1 grams per liter |
Can find out that through last table 1 ability that solvating agent of the present invention is removed protein substance obviously is superior to control group.
Embodiment 2
Present embodiment is the preferred embodiment of substratum and preparation method thereof.
Substratum is made by solvating agent dissolved solids raw material, wherein
Solid material comprises peptone 10 grams, Carnis Bovis seu Bubali cream 3 grams, sodium-chlor 5 grams, Sodium Citrate 3 grams and agar 0.5 gram.
Solvating agent is dissolved in 1000 ml distilled waters by Sodium Citrate 5 gram, sodium-chlor 7 grams, sal epsom 10 grams, para-amino benzoic acid 2 grams, 4-aminoantipyrene 3 grams, Sodium phosphate, dibasic 5 grams and potassium primary phosphate 3 grams and makes.
Per 21.5 grams of above-mentioned solid material were stirred 1～2 minute with 10～20 milliliters of solvating agents (being preferably 11～15 milliliters); Be mixed into pasty state; Add zero(ppm) water to 1000 milliliter again, left standstill after mixing 3-5 minute, then with the pH value to 7.4 of ionocolorimeter regulator solution.
Above-mentioned culture medium solution is carried out packing by general consumption, and general amount by 50 milliliters every part is loaded in the liquid bottles.Liquid bottles is used the aluminium lid press seal after sealing with soft rubber ball again.
High-pressure sterilizing pot is adopted in sterilization, gets final product in 15 minutes with the lasting sterilization of the pressure of 0.7-1 kilogram.Obtain substratum of the present invention.
The mould antibiotic effect that is used for neutralizing of penicillium mould that also can add 100 units in the above-mentioned per 50 milliliters substratum.
Above-mentioned substratum at room temperature stores for future use and gets final product.The usefulness that can be used for blood, marrow cultivation and clinical bacteriology check at any time.
Substratum described in the CN101144068A that mentions in substratum of the present invention and the background technology is compared, and it is fast to have dissolution rate, the characteristics of good stability.Specifically see the following form 2.
Table 2
| Dissolution rate | Stability | |
| Substratum (background technology) | 30-60 minutes | 45 days |
| Substratum (the present invention) | 5-10 minute | 60 days |
Above embodiment is merely exemplary embodiment of the present invention, is not used in restriction the present invention, and protection scope of the present invention is defined by the claims.Those skilled in the art can make various modifications or be equal to replacement the present invention in essence of the present invention and protection domain, this modification or be equal to replacement and also should be regarded as dropping in protection scope of the present invention.
Claims (8)
1. a solvating agent is characterized in that, its effective ingredient is:
2. solvating agent according to claim 1 is characterized in that, the effective ingredient of said solvating agent is:
3. the preparation method of a solvating agent; It is characterized in that; Comprise the steps: to weigh up each component, each components dissolved is got final product in 1000 ml distilled waters by the proportioning of Sodium Citrate 3～7 grams, sodium-chlor 5～9 grams, sal epsom 8～12 grams, para-amino benzoic acid 1～4 gram, 4-aminoantipyrene 1～5 gram, Sodium phosphate, dibasic 3～7 grams, potassium primary phosphate 1～5 gram.
4. the preparation method of solvating agent according to claim 3; It is characterized in that, said each component to take by weighing proportioning following: Sodium Citrate 5 grams, sodium-chlor 7 grams, sal epsom 10 grams, para-amino benzoic acid 2 grams, 4-aminoantipyrene 3 grams, Sodium phosphate, dibasic 5 grams, potassium primary phosphate 3 grams.
5. substratum is made by solvating agent dissolved solids raw material, it is characterized in that, wherein
Solid material comprises peptone 10 mass parts, Carnis Bovis seu Bubali cream 3 mass parts, sodium-chlor 5 mass parts, Sodium Citrate 3 mass parts and agar 0.5 mass parts;
Solvating agent is dissolved in by Sodium Citrate 3～7 mass parts, sodium-chlor 5～9 mass parts, sal epsom 8～12 mass parts, para-amino benzoic acid 1～4 mass parts, 4-aminoantipyrene 1～5 mass parts, Sodium phosphate, dibasic 3～7 mass parts and potassium primary phosphate 1～5 mass parts and makes the solution that concentration is 35 grams per liters in the zero(ppm) water.
6. substratum according to claim 5; It is characterized in that said solvating agent makes the solution that concentration is 35 grams per liters for being dissolved in by Sodium Citrate 5 mass parts, sodium-chlor 7 mass parts, sal epsom 10 mass parts, para-amino benzoic acid 2 mass parts, 4-aminoantipyrene 3 mass parts, Sodium phosphate, dibasic 5 mass parts and potassium primary phosphate 3 mass parts in the zero(ppm) water.
7. the preparation method of claim 5 or 6 substratum is characterized in that the preparation method of above-mentioned substratum; Comprise the steps: to take by weighing in proportion solid material, and the preparation solvating agent, per 21.5 grams of above-mentioned solid material are mixed into pasty state with 10～20 milliliters of solvating agents; Add zero(ppm) water to 1000 milliliter again; Left standstill 3-5 minute, and regulated pH value to 7.4, promptly get substratum after the sterilization.
8. the preparation method of substratum according to claim 7 is characterized in that, per 21.5 grams of said solid material are mixed into pasty state with 15 milliliters of solvating agents.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101556887A CN102337184B (en) | 2011-06-09 | 2011-06-09 | Solvent, preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101556887A CN102337184B (en) | 2011-06-09 | 2011-06-09 | Solvent, preparation method and application thereof |
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| Publication Number | Publication Date |
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| CN102337184A CN102337184A (en) | 2012-02-01 |
| CN102337184B true CN102337184B (en) | 2012-11-07 |
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| CN2011101556887A Active CN102337184B (en) | 2011-06-09 | 2011-06-09 | Solvent, preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113322055B (en) * | 2020-02-28 | 2023-06-20 | 中国石油化工股份有限公司 | Dissolving agent and application thereof in dissolving well cementation sliding sleeve |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3579423A (en) * | 1968-12-13 | 1971-05-18 | Flow Lab | Preparation of tissue culture media |
| CN101144068A (en) * | 2007-09-03 | 2008-03-19 | 王惠萱 | Fast dissolution preparation method for blood culture medium |
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2011
- 2011-06-09 CN CN2011101556887A patent/CN102337184B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3579423A (en) * | 1968-12-13 | 1971-05-18 | Flow Lab | Preparation of tissue culture media |
| CN101144068A (en) * | 2007-09-03 | 2008-03-19 | 王惠萱 | Fast dissolution preparation method for blood culture medium |
Non-Patent Citations (1)
| Title |
|---|
| 王惠萱.基础肉汤培养基速溶配制法.《西南国防医药》.1991,(第2期), * |
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Effective date of registration: 20230105 Address after: 650000 floor 2, building 4, pharmaceutical technology R & D base, intersection of Haiyuan North Road and Keji Road (Yunnan Haobang Investment Co., Ltd.), Kunming, Yunnan Patentee after: Yunnan Dean Medical Laboratory Co.,Ltd. Address before: No. 212, Daguan Road, Kunming, Yunnan 650032 - Laboratory Department of Kunming General Hospital, Military Region Patentee before: Wang Huixuan |