CN104095151B - A kind of citrus fruit fly larva meridic diet and preparation method thereof - Google Patents
A kind of citrus fruit fly larva meridic diet and preparation method thereof Download PDFInfo
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Abstract
A kind of citrus fruit fly larva meridic diet and preparation method thereof, this meridic diet is made up of the raw material of following proportioning: analysis for soybean powder 20～30g, Semen Maydis powder 20～30g, sugar 5～10g, yeast 4～8g, vitamin 0.3～0.4g, acid 0.05～0.1mL, fresh Citrus sarcocarp 10～15g, Spirulina powder 0～0.5g, fresh orange blossom 8～12mL, toilet paper 2～5g, water 120～140mL, preservative 0.08～0.2g.Present invention additionally comprises the preparation method of described citrus fruit fly larva meridic diet.The meridic diet formula materials of the present invention is easy to get, low cost, and preparation method is simple, can mass rearing citrus fruit fly larva.
Description
Technical field
The present invention relates to a kind of meridic diet and preparation method thereof, especially relate to a kind of citrus fruit fly larva with half
Man-made feeds and preparation method thereof.
Background technology
Citrus fruit flyBactrocera minax(Enderlein) Diptera (Diptera) Tephritidae is belonged to
(Tephritidae), being the crushing insect of Citrus, this worm is distributed mainly on Hunan, Hubei, Sichuan, Chongqing, cloud in China
The Citrus producing regions such as south, Guizhou.There is a generation in 1 year in this worm, it lays eggs in citrusfruit, and larva is bored in moth Fructus Citri tangerinae fruit with Fructus Citri tangerinae lobe fruit
Meat is food, causes the underdone first Huang of wormed fruit to come off in advance, loses edibility, fruit losses rate about 20-30%, and production loss is serious
Shi Keda 70%-80% is down to 100%, it has also become the first insect pest of China's Orange Producing, the situation is tense in prevention and control.The biggest to Citrus
The research of trypetid, primarily rest on Natural Population or utilize natural feed (citrusfruit) raise study its biological characteristics,
The aspects such as occurrence characteristic, behavior and prophylactico-therapeutic measures.And carry out the artificial breeding of citrus fruit fly, optimize citrus fruit fly larva people
Work breeding technology, sets up stable indoor population, it is provided that a large amount of health of neat and consistent of growing, for examination worm source, are to carry out further
The basis of citrus fruit fly Physiology and biochemistry, hereditism, toxicology and study on prevention thereof.Artificial from starting calliphora vomitoria in 1908
After feed formula, the research of insecticide artificial breeding gradually grows up, and up to now, has 1000 various insects both at home and abroad permissible
Application man-made feeds are raised and are bred.1956, Finney fresh carrot, sucrose, torula, antibiotics composition
Larva artificial feed has successfully fed citrus fruit fly.A lot of scholars, through years of researches, can use Semen Maydis powder, sugar, ferment the most
Mass rearing citrus fruit fly, Mediterranean fruitfly and the melon trypetids etc. such as mother.But about citrus fruit fly man-made feeds, it is up till now
Only, only report about its adult man-made feeds (" man-made feeds are to citrus fruit fly Life of Adult and numerous for Wang little Jiao etc.
Grow the impact of characteristic ", see hubei agricultural science, 2012,51(11): 2222-2224), about its larva artificial feed formula and
Artificial diet technique, there is not yet any report so far.
Summary of the invention
The technical problem to be solved in the present invention is to provide that a kind of material is easy to get, preparation method is simple, low cost, can be a large amount of
Raise citrus fruit fly larva meridic diet of citrus fruit fly and preparation method thereof.
The present invention solves its technical problem and the technical scheme is that
The citrus fruit fly larva meridic diet of the present invention, is made up of the raw material of following proportioning: analysis for soybean powder 20～
30g, Semen Maydis powder 20～30g, sugar 5～10g, yeast 4～8g, vitamin 0.3～0.4g, acid 0.05～0.1mL, fresh citrus fruit
Meat 10～15g, Spirulina powder 0～0.5g, fresh orange blossom 8～12mL, toilet paper 2～5g, water 120～140mL, preservative
0.08～0.2g；
Described sugar can be at least one in white sugar, brown sugar, crystal sugar, maltose, sucrose, glucose, preferably white sugar；
Described yeast can be yeast extract, yeast powder, torula, beer yeast, Chinese liquor yeast or dry yeast, preferred yeast
Cream；
Described vitamin can be compound vitamin, vitamin C (ascorbic acid) or 21 JIWEITA, preferably compound vitamin；
Described acid can be in hydrochloric acid, citric acid, malic acid, tartaric acid, oxalic acid, succinic acid, lactic acid, formic acid, acetic acid, butanoic acid
At least one, preferably hydrochloric acid；Add the pH of acid 0.05～0.1mL regulation solution so that it is for faintly acid；
Described fresh Citrus can be Citrus aurantium Linn., Fructus Citri sinensis, Citrus sinensis Osbeck, crystal sugar orange, mandarin orange, Citrus chachiensis Hort., red Fructus Citri tangerinae, Fructus Citri grandis, Fructus Citri Limoniae, Fructus Citri or
Fructus Citri Sarcodactylis, preferably Citrus sinensis Osbeck；
Described water can be distilled water, tap water, mineral water, pure water or distilled water, preferably distilled water；
Described preservative can be benzoic acid, to hydroxy-methylbenzene, sodium benzoate, sorbic acid, potassium sorbate, calcium propionate, propanoic acid
In sodium, methyl parahydroxybenzoate, dehydroactic acid, sodium diacetate, nitrate, nitrite, sodium lactate, sodium propionate at least one
Kind, preferably benzoic acid and to the mixture that hydroxy-methylbenzene mass ratio is 1:0.8～1.2；
Described Semen Maydis powder, vitamin, sugar and spirulina are commercially available, and vitamin and spirulina grind to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described citrus fruit fly larva also can add agar with meridic diet, to utilize molding；
Described toilet paper usable fibers element replaces.
Described fresh Citrus sarcocarp and the acquisition methods of fresh orange blossom: fresh citrusfruit goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus are cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes, it is thus achieved that filtrate is fresh orange juice.
The preparation method of the citrus fruit fly larva meridic diet of the present invention, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20～30g, Semen Maydis powder 20～30g, sugar 5～10g, yeast 4～8g, vitamin
0.3～0.4g, acid 0.05～0.1mL, fresh Citrus sarcocarp 10～15g, Spirulina powder 0～0.5g, fresh orange blossom 8～
12mL, toilet paper 2～5g, water 120～140mL, preservative 0.08～0.2g；
(2) by the analysis for soybean powder weighed up, Semen Maydis powder and sugar mixing；
(3) fetch water with beaker, heating, it is subsequently placed into and weighs toilet paper that is good and that tear up, boil while stir, until water boiling
Rise；
(4) analysis for soybean powder, Semen Maydis powder and the sugar mixture that mix through step (2) are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 48～52 DEG C, admix rapidly Spirulina powder,
Vitamin, yeast, fresh Citrus sarcocarp, fresh orange blossom, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add acid and preservative, fully stir evenly,.
The citrus fruit fly larva meridic diet of the present invention, with a certain amount of analysis for soybean powder, Semen Maydis powder, sugar, yeast, dimension
Raw Citrus sarcocarp plain, sour, fresh, fresh orange blossom, spirulina, water and preservative, i.e. can successfully raise Citrus big in indoor
Trypetid larva, this meridic diet formula materials is easy to get, low cost, and preparation method is simple, can mass rearing citrus fruit fly children
Worm.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.To the inventive method, step or
Amendment that condition is made or replacement, belong to protection scope of the present invention.
Raw material used in embodiment, unless otherwise indicated, is commercial goods.
Embodiment I
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 20g, white sugar 5g, yeast extract 4g, compound vitamin 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh umbilicus
Fructus Citri junoris meat 10g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzoic acid
With 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 20g, white sugar 5g, yeast extract 4g, compound vitamin 0.3g, hydrochloric acid
0.05mL, fresh Citrus sinensis Osbeck sarcocarp 10g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anticorrosion
Agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment II
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 30g,
Semen Maydis powder 20g, white sugar 5g, yeast extract 4g, compound vitamin 0.4g, hydrochloric acid (concentration of hydrochloric acid is 36wt%) 0.1mL, fresh umbilicus
Fructus Citri junoris meat 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzoic acid
With 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 30g, Semen Maydis powder 20g, white sugar 5g, yeast extract 4g, compound vitamin 0.4g, hydrochloric acid
0.1mL, fresh Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative
0.12g；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment III
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 30g,
Semen Maydis powder 20g, white sugar 10g, yeast extract 8g, compound vitamin 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh
Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzene first
Acid and 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 30g, Semen Maydis powder 20g, white sugar 10g, yeast extract 8g, compound vitamin 0.3g, salt
Acid 0.05mL, fresh Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anti-
Rotten agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment IV
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 20g, white sugar 10g, yeast extract 8g, compound vitamin 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.1mL, fresh umbilicus
Fructus Citri junoris meat 10g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzoic acid
With 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 20g, white sugar 10g, yeast extract 8g, compound vitamin 0.4g, salt
Acid 0.1mL, fresh Citrus sinensis Osbeck sarcocarp 10g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anticorrosion
Agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment V
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 30g,
Semen Maydis powder 30g, white sugar 5g, yeast extract 8g, compound vitamin 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh umbilicus
Fructus Citri junoris meat 10g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzoic acid
With 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 30g, Semen Maydis powder 30g, white sugar 5g, yeast extract 8g, compound vitamin 0.4g, hydrochloric acid
0.05mL, fresh Citrus sinensis Osbeck sarcocarp 10g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anticorrosion
Agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment VI
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 30g, white sugar 5g, yeast extract 8g, compound vitamin 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.1mL, fresh umbilicus
Fructus Citri junoris meat 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzoic acid
With 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 30g, white sugar 5g, yeast extract 8g, compound vitamin 0.3g, hydrochloric acid
0.1mL, fresh Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative
0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 52 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment VII
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh
Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzene first
Acid and 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, salt
Acid 0.05mL, fresh Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anti-
Rotten agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 48 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment VIII
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 30g,
Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.1mL, fresh umbilicus
Fructus Citri junoris meat 10g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzoic acid
With 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 30g, Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.3g, salt
Acid 0.1mL, fresh Citrus sinensis Osbeck sarcocarp 10g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anticorrosion
Agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment IX
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh
Citrus sinensis Osbeck sarcocarp 15g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzoic acid and to hydroxyl first
The each 0.06g of benzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.3g, salt
Acid 0.05mL, fresh Citrus sinensis Osbeck sarcocarp 15g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzene first
Acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment X
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh
Citrus sinensis Osbeck sarcocarp 15g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzoic acid and to hydroxyl first
The each 0.06g of benzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, salt
Acid 0.05mL, fresh Citrus sinensis Osbeck sarcocarp 15g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzene first
Acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment XI
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh
Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.1g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzene first
Acid and 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, salt
Acid 0.05mL, fresh Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.1g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anti-
Rotten agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment XII
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.3g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh
Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.2g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzene first
Acid and 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, salt
Acid 0.05mL, fresh Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.2g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anti-
Rotten agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment XIII
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh
Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.3g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzene first
Acid and 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, salt
Acid 0.05mL, fresh Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.3g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anti-
Rotten agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment XIV
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh
Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzene first
Acid and 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, salt
Acid 0.05mL, fresh Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.4g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anti-
Rotten agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Embodiment XV
The citrus fruit fly larva meridic diet of the present embodiment, is made up of the raw material of following proportioning: analysis for soybean powder 20g,
Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, hydrochloric acid (concentration of hydrochloric acid is 38wt%) 0.05mL, fresh
Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.5g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, preservative 0.12g(benzene first
Acid and 0.06g each to hydroxy-methylbenzene)；
Described Semen Maydis powder, compound vitamin, white sugar are commercially available, and compound vitamin grinds to form powdery；
Described analysis for soybean powder is commercially available Semen Glycines and breaks into powdery；
Described fresh Citrus sinensis Osbeck sarcocarp and the acquisition methods of fresh naval orange juice: fresh Navel Orange Fruits goes peel, take out Fructus Citri tangerinae lobe
Remove endocarp, obtain fresh Citrus sinensis Osbeck sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus sinensis Osbeck is cut in half
Directly extruding, the juice of extrusion filters with 60 mesh gauzes), it is thus achieved that filtrate is fresh Citrus sinensis Osbeck fruit juice.
The preparation method of the citrus fruit fly larva meridic diet of the present embodiment, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20g, Semen Maydis powder 30g, white sugar 10g, yeast extract 4g, compound vitamin 0.4g, salt
Acid 0.05mL, fresh Citrus sinensis Osbeck sarcocarp 15g, Spirulina powder 0.5g, fresh naval orange juice 10mL, medicated napkin 3g, distilled water 120mL, anti-
Rotten agent 0.12g(benzoic acid and 0.06g each to hydroxy-methylbenzene)；
(2) analysis for soybean powder weighed up, Semen Maydis powder and white sugar are mixed；
(3) distilled water is taken with beaker, heating, it is subsequently placed into and weighs medicated napkin that is good and that tear up, boil while stir, until water
Boiling；
(4) analysis for soybean powder mixed through step (2), Semen Maydis powder and white sugar mixture are poured in step (3) gained boiling water, stir
Even, obtain culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 50 DEG C, admixes rapidly Spirulina powder, be combined
Vitamin, yeast extract, fresh Citrus sinensis Osbeck sarcocarp, fresh naval orange juice, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add hydrochloric acid and preservative, fully stir evenly, to obtain final product.
Feed and test:
(every 10 is a repetition, weight to feed citrus fruit fly larva with embodiment of the present invention I～XV gained meridic diet
Multiple 6～15 times), larva number that each embodiment is fed and the average viability of 10d after raising, raise after the body length of 10d average
Increment, and the most always percentage of pupation is shown in Table 1.
Table 1 embodiment I～XV gained meridic diet feed the result data of citrus fruit fly larva
Note: in table 1, every 10 is a repetition.Wherein,
Average viability (%): represent in each repetition, survival larva number accounts for the percentage rate for examination larva number；
The long average increasing amount of body (mm/ head): represent in each repetition, survival larva the long increment of average body (millimeter/
Head)；
Average total percentage of pupation (%): representing in each repetition, borer population of pupating accounts for the percentage rate for examination larva number；
In table, with lower case difference person after column data, it is poor through the SPSS Software of Data Statistics new multipole of Duncan ' s to represent
Multiple comparison graph is checked, notable at 0.05 level difference.
Claims (8)
1. a citrus fruit fly larva meridic diet, it is characterised in that be made up of the raw material of following proportioning: analysis for soybean powder 20
～30g, Semen Maydis powder 20～30g, sugar 5～10g, yeast 4～8g, vitamin 0.3～0.4g, acid 0.05～0.1mL, fresh Citrus
Sarcocarp 10～15g, Spirulina powder 0～0.5g, fresh orange blossom 8～12mL, toilet paper 2～5g, water 120～140mL, preservative
0.08～0.2g；
Described preservative be benzoic acid, to hydroxy-methylbenzene, sodium benzoate, sorbic acid, potassium sorbate, calcium propionate, sodium propionate, to hydroxyl
At least one in yl benzoic acid methyl ester, dehydroactic acid, sodium diacetate, nitrate, nitrite, sodium lactate, sodium propionate；
Described fresh Citrus sarcocarp and the acquisition methods of fresh orange blossom: fresh citrusfruit goes peel, take out Fructus Citri tangerinae lobe and remove
Endocarp, obtains fresh Citrus sarcocarp；The Fructus Citri tangerinae lobe taken out is wrapped up with 60 mesh gauzes, extruding, or Citrus are cut in half directly
Extruding, the juice of extrusion filters with 60 mesh gauzes, it is thus achieved that filtrate is fresh orange juice；
The preparation method of described citrus fruit fly larva meridic diet, comprises the following steps:
(1) weigh or measure: analysis for soybean powder 20～30g, Semen Maydis powder 20～30g, sugar 5～10g, yeast 4～8g, vitamin 0.3～
0.4g, acid 0.05～0.1mL, fresh Citrus sarcocarp 10～15g, Spirulina powder 0～0.5g, fresh orange blossom 8～12mL, health
Paper 2～5g, water 120～140mL, preservative 0.08～0.2g；
(2) by the analysis for soybean powder weighed up, Semen Maydis powder and sugar mixing；
(3) fetch water with beaker, heating, it is subsequently placed into and weighs toilet paper that is good and that tear up, boil while stir, until water boiling；
(4) analysis for soybean powder, Semen Maydis powder and the sugar mixture that mix through step (2) are poured in step (3) gained boiling water, stir evenly,
Culture medium；
(5) by step (4) gained culture medium natural cooling, when temperature is down to 48～52 DEG C, Spirulina powder, dimension life are admixed rapidly
Element, yeast, fresh Citrus sarcocarp, fresh orange blossom, stir evenly；
(6) after the culture medium processed through step (5) cools down completely, add acid and preservative, fully stir evenly,.
Citrus fruit fly larva meridic diet the most according to claim 1, it is characterised in that described sugar be white sugar,
At least one in brown sugar, crystal sugar, maltose, sucrose, glucose.
Citrus fruit fly larva meridic diet the most according to claim 1 and 2, it is characterised in that described yeast is
Yeast extract, yeast powder, torula, dry yeast, beer yeast or Chinese liquor yeast.
Citrus fruit fly larva meridic diet the most according to claim 1 and 2, it is characterised in that described vitamin
For compound vitamin, vitamin C or 21 JIWEITA.
Citrus fruit fly larva meridic diet the most according to claim 1 and 2, it is characterised in that described acid is salt
At least one in acid, citric acid, malic acid, tartaric acid, oxalic acid, succinic acid, lactic acid, formic acid, acetic acid, butanoic acid.
Citrus fruit fly larva meridic diet the most according to claim 1 and 2, it is characterised in that described fresh Citrus chachiensis Hort.
Fructus Citri tangerinae is Citrus aurantium Linn., Fructus Citri sinensis, Citrus sinensis Osbeck, crystal sugar orange, mandarin orange, Citrus chachiensis Hort., red Fructus Citri tangerinae, Fructus Citri grandis, Fructus Citri Limoniae, Fructus Citri or Fructus Citri Sarcodactylis.
Citrus fruit fly larva meridic diet the most according to claim 1 and 2, it is characterised in that described water is for steaming
Distilled water, tap water, mineral water, pure water or distilled water.
Citrus fruit fly larva meridic diet the most according to claim 1 and 2, it is characterised in that described preservative
For benzoic acid with to the mixture that hydroxy-methylbenzene mass ratio is 1:0.8～1.2.
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| CN105494411B (en) * | 2015-12-18 | 2018-01-16 | 莫博程 | A kind of citrus fruit fly adult attractant and its application method |
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| CN102987165A (en) * | 2012-12-27 | 2013-03-27 | 福建农林大学 | South Asia trypetid larva artificial feed |
| CN103300257B (en) * | 2013-07-09 | 2015-05-06 | 海南出入境检验检疫局热带植物隔离检疫中心 | Artificial feed for bactrocera dorsalis larvas |
| CN103315183B (en) * | 2013-07-10 | 2015-05-20 | 海南出入境检验检疫局热带植物隔离检疫中心 | Artificial feed for melon fly larvae |
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2013
- 2013-04-02 CN CN201310112622.9A patent/CN104095151B/en active Active
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