CN104431439A - Artificial feed for adult bactrocera minax, and preparation method and application thereof - Google Patents
Artificial feed for adult bactrocera minax, and preparation method and application thereof Download PDFInfo
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- CN104431439A CN104431439A CN201410661188.4A CN201410661188A CN104431439A CN 104431439 A CN104431439 A CN 104431439A CN 201410661188 A CN201410661188 A CN 201410661188A CN 104431439 A CN104431439 A CN 104431439A
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Abstract
The invention discloses an artificial feed for adult bactrocera minax, and a preparation method and application thereof. The artificial feed is prepared from the following materials: 24-36g of brown sugar, 8-12g of yeast extract powder or/and yeast paste or/and yeast powder and 200-400g of distilled water. The invention also discloses the preparation method and the application of the artificial feed. The artificial feed disclosed by the invention has the advantages that the materials are easily available, the operation is simple, the cost is low, the life of adult bactrocera minax is long, the fertility of adult bactrocera minax is high and the environmental-friendly effect is achieved.
Description
Technical field
The present invention relates to a kind of citrus fruit fly man-made feeds and preparation method thereof and application, especially relate to a kind of man-made feeds and preparation method thereof and application.
Background technology
Citrus fruit fly
bactrocera minax(Enderlein) belong to Diptera (Diptera) Tephritidae (Tephritidae), be the crushing insect of oranges and tangerines, be mainly distributed in the areas such as Hunan, Hubei, Sichuan, Chongqing, Yunnan, Guizhou in China.Within 1 year, there is a generation in this worm, it lays eggs in citrusfruit, larva bore moth tangerine fruit in tangerine lobe pulp for food, the underdone first Huang of wormed fruit is caused to come off in advance, lose edibility, fruit losses rate is about 20-30%, can reach 70%-80% so that 100% when production loss is serious, become the first insect pest of China's Orange Producing, the situation is tense in prevention and control.
Carry out the research of citrus fruit fly prevention and control measure, need the experimental population setting up artificial feeding, for the smooth enforcement of this research provides safeguard.In addition, because the ovum of this worm, larva and pupa life area are hidden, wherein ovum and larva live in citrusfruit, pupa is in soil, control is got up more difficult, therefore, the adult stage of free living has just become the key period of control, needs a large amount of healthy adult to ensure carrying out smoothly of every experimental study, therefore, the raising in adult stage is also of crucial importance.
The adult of citrus fruit fly is from emergence to mate and oviposit, need the particular time through nutritional supplementation, supplementing of nutrition directly has influence on its fertility and life-span, screening and optimizing citrus fruit fly adult man-made feeds, in laboratory, carry out the important ring that the artificial feeding of citrus fruit fly and population continue generation breeding, can be the fundamental biological knowledge research of citrus fruit fly, and further microcosmic electro physiology, molecular biology, the worm source that toxicology and the research of various prevention and controls provide source consistent, and further for the formulation of its prevention and control measure and the development of adult lure (comprising food lure and gyplure) provide foundation.
The man-made feeds of citrus fruit fly adult, only there is one section of report of (2012) such as Wang little Jiao at present, have studied hydromel, sugar-vinegar liquid, brewer's yeast sucrose water and peptone sucrose water etc., the 23-45 days but the adult that these man-made feeds are fed only can survive, and egg laying amount is lower.
Summary of the invention
The technical problem to be solved in the present invention is, overcomes the deficiencies in the prior art, provides a kind of raw material to be easy to get, with low cost, citrus fruit fly adult man-made feeds that preparation method is easy and preparation method thereof and application, the citrus fruit fly Life of Adult that its man-made feeds are raised is longer, and egg laying amount is higher.
The technical scheme that the present invention solves the employing of its technical problem is that the citrus fruit fly adult man-made feeds of the present invention, make primarily of following raw material: brown sugar 24-36g, and yeast soaks powder or/and yeast extract is or/and dusty yeast 8-12g, distilled water 200-400g.
Preferred material rate is brown sugar: yeast leaching powder is or/and yeast extract is or/and dusty yeast: distilled water=3:1:30.
Described brown sugar also can adopt honey, white sugar or sucrose to replace.
Described yeast leaching powder is or/and yeast extract is or/and dusty yeast also can with dry ferment or/and brewer's yeast replaces.
The preparation method of the citrus fruit fly man-made feeds of the present invention, comprises the following steps:
(1) raw materials weighing: brown sugar 24-36g, yeast soaks powder or/and yeast extract is or/and dusty yeast 8-12g, distilled water 200-400g;
(2) after distilled water is boiled, add brown sugar, after being stirred to dissolving, treat that it naturally cools to 45-50 DEG C, add yeast leaching powder or/and yeast extract is or/and dusty yeast, fully stir evenly, to obtain final product.
The application of the citrus fruit fly man-made feeds of the present invention, namely utilizes the man-made feeds of the present invention to raise the method for citrus fruit fly, comprises the following steps:
Medical cotton is pulled into microscler, with filter paper packet into strips, insert with cover in the middle of perforation, fill in the disposable little plastic cup of liquid man-made feeds, strip cotton core one end is inserted in man-made feeds liquid, and the other end exposes the length covering outer 0.5-1cm; This device is placed in cage inner bottom part, makes nutrient solution pass cotton core for citrus fruit fly imago feeding, prevent worm wing from being clung by nutrient solution and causing the death of worm; Hang over top in cage by unsettled for the little plastic sheet dispersion being moistened with liquid man-made feeds simultaneously, for imago feeding, do not drip with liquid and be advisable, stick on liquid when preventing adult from creeping.There is provided potted plant oranges and tangerines seedling to stop for citrus fruit fly adult, every 2-4 days (preferably 3 days) add a feed, and clean and change cotton core and cup.
Raise indoor conditions: periodicity of illumination L:D=14h:10h, temperature 25 ± 2 DEG C; Every day, according to indoor humidity situation, takes the circumstances into consideration to spray water 3-5 time toward dependent insect cage inwall, notes, not directly facing to citrus fruit fly spray, in cage, separately hanging the cotton balls of immersion, for adult provides drinking-water, and holding chamber humidity 60 ~ 75%.
Adopt the adult man-made feeds of the present invention to raise citrus fruit fly, the longlyest adult can be made to survive reach 53 days, single female egg laying amount reaches as high as 26.15/head.
The citrus fruit fly adult man-made feeds of the present invention, raw material is easy to get, easy and simple to handle, with low cost, and raising citrus fruit fly Life of Adult is longer, and reproductive capacity is higher, environmental friendliness.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment A formula brown sugar: yeast leaching powder: distilled water=3:1:20 (W/W/W)
Embodiment B formula sucrose: yeast leaching powder: distilled water=3:1:20(W/W/W)
Embodiment C formula brown sugar: yeast leaching powder: distilled water=3:1:30(W/W/W)
Embodiment D fills a prescription sucrose: yeast leaching powder: distilled water=3:1:30(W/W/W)
Embodiment E formula brown sugar: yeast leaching powder: distilled water=3:1:40(W/W/W)
Embodiment F formula sucrose: yeast leaching powder: distilled water=3:1:40(W/W/W)
The preparation method of the citrus fruit fly man-made feeds of the present embodiment, comprises the following steps:
(1) raw materials weighing;
(2) after distilled water is boiled, add brown sugar or the sucrose of corresponding formula rate, after being stirred to dissolving, treat that it naturally cools to 50 DEG C, add the yeast leaching powder of setting ratio, fully mix thoroughly, to obtain final product.
The application of the citrus fruit fly man-made feeds of the present embodiment, namely utilizes the man-made feeds of the present embodiment to raise the method for citrus fruit fly, comprises the following steps:
Medical cotton is pulled into microscler, with filter paper packet into strips, insert the middle perforation of lid, fill in the disposable little plastic cup of liquid man-made feeds of the present embodiment, strip cotton core one end is inserted in man-made feeds liquid, the other end exposes the length covering outer 0.5-1cm, make nutrient solution pass cotton core for citrus fruit fly imago feeding, prevent worm wing from being clung by nutrient solution and causing the death of worm, cup is placed in cage inner bottom part; Hang over top in cage and provide feed by unsettled for the little plastic sheet dispersion being moistened with liquid feed for adult simultaneously, do not drip with liquid and be advisable, stick on liquid when preventing adult from creeping, and provide potted plant oranges and tangerines seedling to stop for adult.
The citrus fruit fly adult recently sprouted wings caught the same period puts into 6 dependent insect cages (500mm*500mm*500mm) at random respectively, 30, every cage, and the one formula in the nutritional supplementation liquid of filling a prescription with above-mentioned different proportion raised by every cage.Within every 3 days, add replacing fresh feed, and clean and change cotton core and cup.Operate the same.Every day observed and recorded citrus fruit fly adult activity and survival condition, remove dead worm in time, add up remaining survival borer population.
In addition, get the adult recently sprouted wings and to move in 6 dependent insect cages same as described above (every cage female, each 15 of male worm), oranges and tangerines branch with fruit (diameter is 10mm ~ 30mm) is inserted in the mineral water bottle of band water, bottleneck cotton balls clogs (avoiding adult to fall into drowned), and the same method provides 6 kinds to fill a prescription man-made feeds in each cage respectively.Every 3d changes new branch, and each every cage is supplied to 4 ~ 6, the branch of citrus fruit fly band fruit, totally 10 fruits.Change the fruit of branch, find trace of laying eggs, along laying eggs, trace cuts fruit, statistics ovum amount.
Insectary condition remains on periodicity of illumination L:D=14h:10h, temperature 25 ± 2 DEG C.Every day, timing was sprinkled water in insectary, used air humidifier to increase indoor humidity.And take the circumstances into consideration every day to spray water 3-5 time toward dependent insect cage inwall, in cage, separately hang the cotton-wool of immersion, provide drinking-water to adult.Indoor humidity 60 ~ 75%.
The impact of embodiment A ~ F different formulations man-made feeds on the survival of citrus fruit fly adult and reproductive capacity the results are shown in Table 1.
Can find out, the man-made feeds of above-mentioned formula all can raise citrus fruit fly adult, but under equal conditions, formula using brown sugar as nucleopolyhedrovirus (A C E), than the citrus fruit fly adult that the formula (B D F) using sucrose as nucleopolyhedrovirus is raised, its Life of Adult and reproductive capacity are all higher.Meanwhile, the formula of different proportion, also has impact to result, with brown sugar: yeast leaching powder: the ratio of water is that the formula of 3:1:30 is the most suitable.
Claims (8)
1. citrus fruit fly adult man-made feeds, is characterized in that, are made up of following raw material: brown sugar 24-36g, and yeast soaks powder or/and yeast extract is or/and dusty yeast 8-12g, distilled water 200-400g.
2. citrus fruit fly adult man-made feeds according to claim 1, is characterized in that, be made up: brown sugar of the raw material of following weight proportion: yeast leaching powder is or/and yeast extract is or/and dusty yeast: distilled water=3:1:30.
3. citrus fruit fly adult man-made feeds according to claim 1 and 2, is characterized in that, described brown sugar adopts honey or/and white sugar is or/and sucrose replaces.
4. citrus fruit fly adult man-made feeds according to claim 1 and 2, is characterized in that, described yeast leaching powder is or/and yeast extract is or/and dusty yeast adopts dry ferment or/and brewer's yeast replaces.
5. prepare a method for citrus fruit fly man-made feeds as described in one of claim 1-4, it is characterized in that, comprise the following steps:
(1) raw materials weighing: brown sugar 24-36g, yeast soaks powder or/and yeast extract is or/and dusty yeast 8-12g, distilled water 200-400g;
(2) after distilled water is boiled, add brown sugar, after being stirred to dissolving, treat that it naturally cools to 45-50 DEG C, add yeast leaching powder or/and yeast extract is or/and dusty yeast, fully stir evenly, to obtain final product.
6. the application of citrus fruit fly man-made feeds as described in one of claim 1-4, is characterized in that, comprise the following steps:
Medical cotton is pulled into microscler, with filter paper packet into strips, insert the middle perforation of lid, fill in the disposable little plastic cup of liquid man-made feeds, strip cotton core one end is inserted in man-made feeds liquid, the other end exposes the length covering outer 0.5-1cm, this device is placed in dependent insect cage inner bottom part, makes nutrient solution pass cotton core and take food for citrus fruit fly; The little plastic sheet dispersion being moistened with liquid man-made feeds is suspended on top in cage, for imago feeding simultaneously; There is provided potted plant oranges and tangerines seedling to stop for citrus fruit fly adult, every 2-4 days adds a feed, and cleans and change cotton core and cup.
7. the application of citrus fruit fly man-made feeds according to claim 6, is characterized in that, within every 3 days, adds a feed.
8. the application of the citrus fruit fly man-made feeds according to claim 6 or 7, is characterized in that,
Raise indoor conditions: periodicity of illumination L:D=14h:10h, temperature 25 ± 2 DEG C; Every day sprays water 3-5 time toward dependent insect cage inwall, directly facing to citrus fruit fly spray, hangs the cotton balls of immersion in dependent insect cage simultaneously, for adult provides drinking-water, and holding chamber humidity 60 ~ 75%.
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| CN201410661188.4A CN104431439A (en) | 2014-11-19 | 2014-11-19 | Artificial feed for adult bactrocera minax, and preparation method and application thereof |
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| CN105166585A (en) * | 2015-10-28 | 2015-12-23 | 浙江省柑桔研究所 | Artificial feed for bactrocera tau adults and preparation method thereof |
| CN106173568A (en) * | 2016-07-22 | 2016-12-07 | 红河学院 | A kind of Fructus Capsici trypetid larva artificial feed and preparation method thereof |
| CN107517934A (en) * | 2017-09-06 | 2017-12-29 | 云南省林业科学院 | A kind of more worm state box for feeding pests of citrus fruit fly and its method for breeding |
| CN110115250A (en) * | 2019-05-10 | 2019-08-13 | 湖南农业大学 | A kind of rearing method and larva artificial feed of the full worm state of citrus fruit fly |
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| CN105166585A (en) * | 2015-10-28 | 2015-12-23 | 浙江省柑桔研究所 | Artificial feed for bactrocera tau adults and preparation method thereof |
| CN106173568A (en) * | 2016-07-22 | 2016-12-07 | 红河学院 | A kind of Fructus Capsici trypetid larva artificial feed and preparation method thereof |
| CN107517934A (en) * | 2017-09-06 | 2017-12-29 | 云南省林业科学院 | A kind of more worm state box for feeding pests of citrus fruit fly and its method for breeding |
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| CN110115250A (en) * | 2019-05-10 | 2019-08-13 | 湖南农业大学 | A kind of rearing method and larva artificial feed of the full worm state of citrus fruit fly |
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