CN103315183A - Artificial feed for melon fly larvae - Google Patents
Artificial feed for melon fly larvae Download PDFInfo
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- CN103315183A CN103315183A CN2013102867191A CN201310286719A CN103315183A CN 103315183 A CN103315183 A CN 103315183A CN 2013102867191 A CN2013102867191 A CN 2013102867191A CN 201310286719 A CN201310286719 A CN 201310286719A CN 103315183 A CN103315183 A CN 103315183A
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Abstract
The invention discloses an artificial feed for melon fly larvae, which comprises the following ingredients: a main agent, a filling agent, a preservative, an acidity regulator and water, wherein the main agent comprises beer yeast and sugar; the filling agent comprises wheat bran, fine corn particles and sanitary pulp; the preservative comprises methyl parahydroxybenzoats; the acidity regulator comprises hydrochloric acid and an alkaline neutralizing agent or an acidic neutralizing agent. The artificial feed disclosed by the invention is simple and efficient in melon fly larvae formula, affordable, practical and easy to operate, completely solves the technical problem of widely artificially feeding melon fly larvae in melon fly rooms, promotes the smooth research on steaming and quarantine inspection treatment measures for tropical fruits such as exported fresh fruit pawpaw and the like, provides an important technical support for the export sale of fresh fruits such as pawpaw in out country, significantly promotes the research and the development of artificial feed for melon flies in China, and provides a large amount of important larvae sources for various future scientific researches on melon flies, and therefore, the artificial feed has significant ecological, economic and social benefits.
Description
Technical field
The invention belongs to agricultural biological technical field, be specifically related to a kind of good melon trypetid larva artificial feed prescription.
Background technology
Melon trypetid Bactrocera cucurbitae Coquillett is commonly called as the pin honeybee, and larva claims the melon maggot, belongs to Diptera Diptera, Tephritidae Tephritidae, Dacinae Dacinae, Bactrocera Bactrocera Macquart.The melon trypetid was in the news in India first in 1913, be distributed widely at present more than 30 countries and regions, subtropical zone, mainly be distributed in the areas such as Guangdong, Guangxi, Hainan, Fujian, Yunnan, Taiwan in China, the sociales of southern trypetid class, its host range is wide, reach 120 various plants, mainly cause harm Curcurbitaceae and plant of Solanaceae are such as muskmelon, cucumber, balsam pear etc.The female adult of melon trypetid is thrust in the young melon epidermis with oopod and lays eggs, the melon that causes being injured thorn injury condensing flow glue, and deformity is sunk, and pericarp is strong, the melon bitter and puckery flavor; Namely pierce to take food in the melon after the larva hatching and cause harm, cause the curcumbitate distortion, rot, cause the melon that falls in a large number, have a strong impact on the quality and yield of melon and fruit.The melon trypetid endangers the loss that 60-70% can be caused in serious area, and local even total crop failure brings serious economic loss to agricultural production, comprises that as important quarantine object is all classified the melon trypetid in a plurality of countries and regions of China.Because China some areas are the epidemic-stricken area of melon trypetid, a lot of fruit of China, vegetables and flower export all are restricted, and have a strong impact on and restrict the fast development of China's agricultural industry.
In recent years, because the tropical fruit (tree)s such as China's mango, pawpaw have the risk that passes with melon trypetid live body, be subject to the restriction of developed country's quarantine regulations such as Japan, for this reason, State General Administration for Quality Supervision's send the documents continuously " carrying out notice from correlation technique work to Japan outlet fruit quarantine market that inject about tissue ", technical barrier is processed in the steaming quarantine that requires inspection and quarantine department to solve early the tropical fruit (tree)s such as outlet pawpaw, squeezes into the international market as early as possible to realize as early as possible the smooth outlets of fresh fruit such as domestic high-quality mango and papaya.Owing to killing the steaming quarantine treatment measures of fresh fruit melon trypetid, need to provide tens thousand of even 1,000,000 physiological standards are consistent for examination worm source, need to carry out standardized melon trypetid man-made feeds mass rearing reproduction technique research.Meanwhile, along with to the deepening continuously and the development of Prevention Technique of the ambits such as biology, physiology, ecology, virulence, pathology, science of heredity and molecular biology of trypetid research, it is test material that the consistent melon trypetid of a large amount of healthy development is needed in the laboratory badly.But because natural feed is subjected to season, the easy effects limit such as rot, so that explore the man-made feeds prescription of melon trypetid, science artificial feeding melon trypetid solves the seasonal shortage problem of melon trypetid, becomes more and more important.
The research of melon trypetid larva artificial feed, foreign study relatively early be the larva artificial feed that Finney forms with fresh carrot, sucrose, torula, antibiotic, but the easy microbial contamination of feed; Chang research is made into liquid feed with brewer's yeast, sucrose, antiseptic and citric acid and raises the melon trypetid, and the adding wheat-germ oil is raised several trypetid larvas in the liquid man-made feeds, but this wheat-germ oil is under-supply on the market, and price is relatively high again.At home, the research of this respect concentrates on the research of citrus fruit fly larva artificial feed mostly, but the formulating for melon trypetid larva artificial feed is less, and the domestic present prescription that uses can't be grown as the melon trypetid provides comprehensive nutrition, can not carry out indoor large-scale artificial feeding, and there is the problem of going mouldy with peculiar smell in the raising later stage, and heavy corrosion Laboratory Instruments equipment affects lab assistant healthy.As domestic at present mainly take single wheat bran as medium, add sucrose and yeast complex larva artificial feed, carry out the artificial feeding of melon trypetid, its egg hatching rate is lower, a lot of larva individualities are less than normal, percentage of pupation and average pupa kind all with under the nature have very big-difference, be difficult to provide that a large amount of growths are consistent, reliable in quality for examination worm source, and raising the later stage, man-made feeds have pollution, mildew phenomena rots, produce very heavy peculiar smell, very easily cause the breeding grub mortality, thereby affect every scientific research of melon trypetid.Thereby develop good, economical and practical melon trypetid larva artificial feed, for every scientific experimentation research of melon trypetid provides that a large amount of growths are consistent, reliable in quality for examination worm source, be the significant task of current melon trypetid artificial feeding technology.
Summary of the invention
The purpose of this invention is to provide a kind of good melon trypetid larva artificial feed.Melon trypetid larva prescription of the present invention is simple efficient, economic and practical, easy operating, thoroughly solved the technical barrier of melon trypetid indoors artificial mass rearing, and acquisition Japanese side approval, solved State Administration of Quality Supervision, Inspection and Quarantine and Hainan Province's emphasis science and technology item " a defeated day mango quarantine steaming Processing Technology Research " and scientific research projects such as " Chinese papaya fresh fruit steaming quarantine Processing Technology Research " for trying the worm source problem, promoted the smooth research of the tropical fruit (tree) steaming quarantine treatment measures such as outlet pawpaw fresh fruit, for the outlet of the fresh fruits such as China pawpaw provides important technical support abroad, advanced extremely significantly the research and development of China's melon trypetid man-made feeds, for the in the future every scientific research of melon trypetid provides a large amount of important worm sources, has significant ecology, economic and social benefit.
To achieve these goals, technical scheme of the present invention is: a kind of melon trypetid larva artificial feed is provided, and composition comprises host, filler, anticorrisive agent, acidity regulator and water, best proportioning and concrete compound method, and its particular content is as follows:
1), host-brewer's yeast and sucrose
Brewer's yeast and sucrose are respectively 25g and 30g, and wherein yeast mainly provides protein and the vitamin that the melon trypetid grows to be needed, and the melon trypetid grows essential B family vitamin; Sucrose is desirable carbon source, and carbohydrate mainly is provided, and is the necessary material that melon trypetid normal growth is grown, on its larva go through the phase, the ontogeny parameters such as survival rate, percentage of pupation, eclosion rate all have appreciable impact.
2), filler-wheat bran, corn particle in small, broken bits, health paper pulp
Filler corn particle 100g in small, broken bits, wheat bran 50g, health paper pulp 20g, for the melon trypetid has been replenished abundant nitrogenous source, part vitamin and crude fibre and unrighted acid, make the quality of feed can satisfy the characteristic of ingesting of its larva, and satisfy the packing action of melon trypetid nutrition quality and quantity.
3), anticorrisive agent-methyl p-hydroxybenzoate
Anticorrisive agent-methyl p-hydroxybenzoate 0.9g is killed various moulds in the feed, prevents that feed is putrid and deteriorated.
4), acidity regulator-hydrochloric acid
Acidity regulator-1mol/L hydrochloric acid 4ml, control feed pH value is 4.0-5.0 approximately, prevents growth of microorganism; Regulate feed viscosity, to adapt to the needs of ingesting.
5), water-400ml
Under normal operation, adopt the man-made feeds prescription after the present invention optimizes: corn particle in small, broken bits (cooking) 100g, wheat bran 50g, sucrose 30g, brewer's yeast 25g, health paper pulp 20g, 1mol/L hydrochloric acid 4ml, anticorrisive agent (methyl p-hydroxybenzoate) 0.9g, can guarantee its larva normal development, can keep again feed not go mouldy lastingly and free from extraneous odour, be suitable for the artificial mass rearing of indoor melon trypetid larva.Wherein corn and wheat bran crop filler can either replenish for larva and enrich comprehensive nutrition, also make the feed modest viscosity, are beneficial to larval growth and grow; The reasonable use of hydrochloric acid and methyl p-hydroxybenzoate amount so that the man-made feeds after optimizing do not go mouldy, has thoroughly been eliminated peculiar smell, has solved corrosion instrument and equipment and the technical barrier that affects the personnel health.Wherein its filial generation incubation rate of this prescription melon trypetid, percentage of pupation, eclosion rate and average pupa heavily are respectively; 78.50 ± 0.04%, 95.73 ± 0.03%, 94.57 ± 0.02 %, 18.62 ± 0.23 mg (table 2) all apparently higher than the man-made feeds prescription of single filler, also obviously are better than the prescription take wheat bran and wheat germ as mixed filler.In 22 kinds of larva artificial feeds of test, obviously be better than man-made feeds prescription with single filler with the mixed filler prescription, wherein mixed filler wheat bran and corn formulation totally are better than wheat germ and wheat bran, wherein the feeding effect of A8, B18 and B22 is better, but the filial generation incubation rate the highest (78.5 ± 0.04%) of B18, next is A5(77.5 ± 0.1%), but all the other majority parameters of prescription B18 all are higher than other prescription, from several indexs comprehensively relatively, the man-made feeds of melon trypetid larva are optimum with prescription B18.
Melon trypetid larva prescription of the present invention is simple efficient, economic and practical, easy operating, thoroughly solved the technical barrier of melon trypetid indoors artificial mass rearing, and acquisition Japanese side approval, solved State Administration of Quality Supervision, Inspection and Quarantine and Hainan Province's emphasis science and technology item " a defeated day mango quarantine steaming Processing Technology Research " and scientific research projects such as " Chinese papaya fresh fruit steaming quarantine Processing Technology Research " for trying the worm source problem, promoted the smooth research of the tropical fruit (tree) steaming quarantine treatment measures such as outlet pawpaw fresh fruit, for the outlet of the fresh fruits such as China pawpaw provides important technical support abroad, advanced extremely significantly the research and development of China's melon trypetid man-made feeds, for the in the future every scientific research of melon trypetid provides a large amount of important worm sources, has significant ecology, economic and social benefit.
The specific embodiment
For describe in detail melon trypetid larva artificial feed of the present invention prescription technology contents, operating process etc., be described further below in conjunction with embodiment.
Melon trypetid man-made feeds prescription, mainly comprise corn particle in small, broken bits (cooking) 100g, wheat bran 50g, sucrose 30g, brewer's yeast 25g, health paper pulp 20g, 1mol/L hydrochloric acid 4ml, anticorrisive agent (methyl p-hydroxybenzoate) 0.9g, its concrete preparation steps is as follows:
1) claim (amount) to get in the prescription various compositions with assay balance and pipettor stand-by;
2) sanitary paper towel is put into breaks into even paper pulp in the automatic pulper;
3) with suitable quantity of water with granulated sugar, brewer's yeast and methyl hydroxybenzoate dissolving, mixing, slowly stir in the impouring paper pulp;
4) stir pouring in the hydrochloric acid again behind the above-mentioned composition mixing;
5) with antalkali (calcium carbonate) or acid acceptor organic acid (acetic acid) man-made feeds that prepare are transferred to the pH value and be 4.0～5.0, for subsequent use;
6) about 10,000 ovum is evenly accessed in the man-made feeds, carry out the larva raising about 20 days.
Be the comparison of other prescription with the present invention's prescription such as following table 1; Table 2 is that the feeding effect of melon trypetid larva corresponding to the various prescriptions of table 1 compares.
Table 1 melon larva artificial feed formula table
Annotate: CK1-CK4 is domestic traditional Single Medium man-made feeds prescription; A5-A13 is wheat germ and wheat bran Latin orthogonal optimization prescription; B14-B22 is corn and wheat bran Latin orthogonal optimization prescription.
Table 2 different formulations man-made feeds are raised melon larva effect relatively
Annotate: data are average ± standard deviations in the table, with indicating different letter representations behind the column data through Duncan ' s duncan's new multiple range method check otherness remarkable (P＜0.05)
Test method and biological parameter statistical description:
1) test method
Get and raise 66 in box, per 22 boxes are that a test repeats, and amount to 3 repetitions.On the paper handkerchief that 200 on every box access ovum is completed in the feed surface, cover with gobo again after covering lid, place under the condition of receptacle and raise.Larva namely pierces in the feed and takes food after the ovum hatching, grows to ripe.
2) percentage of pupation and average pupa are heavy
When most larvae development to 3 during age, it is on the support in the 150 cm basins that the larva raising box is placed diameter, the mature larva that has the spring habit after opening the lid is successively jumped in the water in basin, collect 3 instar larvaes 1 time every 1.5 h, each choosing wherein 100 put into the case of pupating and enter the slightly wet sand bed in bottom for it and pupate, after 3 d pupate to finish, sieve sand and get pupa.Record respectively and add up its pupa number and pupa gross weight, calculate its percentage of pupation heavy with average pupa.
Percentage of pupation (%)=pupa number/confession examination 3 instar larvae number * 100
Heavy (the mg)=pupa gross weight (mg) of average pupa/pupa number
3) eclosion rate
The melon trypetid pupa (counting) of collecting is put into the bottom be covered with the approximately clean wet husky plastic bowl (diameter 10cm) of 2cm of a layer depth, place in the rearging cage, after adult all sprouts wings, check the puparium number of having sprouted wings with record (=sprout wings into borer population) and the pupa number of emergence not, calculate its eclosion rate.
Eclosion rate (%)=sprout wings into borer population/confession to try pupa number * 100
4) filial generation incubation rate
Melon trypetid adult after the emergence is got in 200 accesses of ovum different formulations larva artificial feed and raises after raising enters the egg-laying season, and inspection record hatching larva number calculates its filial generation egg hatching rate behind 6 d.
Filial generation incubation rate (%)=hatching larva number/confession examination ovum number * 100
Above disclosed only is preferred embodiment of the present invention, certainly can not limit with this interest field of the present invention, and the equivalent variations of therefore doing according to claim of the present invention still belongs to the scope that the present invention is contained.
Claims (4)
1. melon trypetid larva artificial feed, it is characterized in that: composition comprises host, filler, anticorrisive agent, acidity regulator and water; Described host comprises brewer's yeast and sucrose; Filler comprises wheat bran, corn particle in small, broken bits and health paper pulp; Anticorrisive agent comprises methyl p-hydroxybenzoate; Acidity regulator comprises hydrochloric acid and antalkali or acid acceptor.
2. melon trypetid larva artificial feed as claimed in claim 1 is characterized in that: described brewer's yeast 25g, sucrose 30g, wheat bran 50g, corn particle 100g in small, broken bits, health paper pulp 20g, 1mol/L hydrochloric acid 4ml, methyl p-hydroxybenzoate 0.9g, water 400ml.
3. melon trypetid larva artificial feed as claimed in claim 1, it is characterized in that: described antalkali is calcium carbonate, acid acceptor is acetic acid.
4. melon trypetid larva artificial feed as claimed in claim 1, it is characterized in that: this melon trypetid larva artificial feed pH value is 4.0～5.0.
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| CN106173568A (en) * | 2016-07-22 | 2016-12-07 | 红河学院 | A kind of Fructus Capsici trypetid larva artificial feed and preparation method thereof |
| CN106306610A (en) * | 2016-08-23 | 2017-01-11 | 张驰 | Feed for larvae of apple flies and preparation method thereof |
| CN106689825A (en) * | 2016-12-05 | 2017-05-24 | 长沙秋点兵信息科技有限公司 | High-hatchability feed for bactrocera mediterranei larvae |
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| CN110613065A (en) * | 2019-07-29 | 2019-12-27 | 海南大学 | Melon fly solid culture medium and preparation method thereof |
| CN115886160A (en) * | 2022-11-30 | 2023-04-04 | 华南农业大学 | Feed for larvae of Bactrocera dorsalis in south Asia |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104095151A (en) * | 2013-04-02 | 2014-10-15 | 湖南师范大学 | Semi-artificial feed for Bactrocera minax larvae and preparation method thereof |
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| CN106173568A (en) * | 2016-07-22 | 2016-12-07 | 红河学院 | A kind of Fructus Capsici trypetid larva artificial feed and preparation method thereof |
| CN106306610A (en) * | 2016-08-23 | 2017-01-11 | 张驰 | Feed for larvae of apple flies and preparation method thereof |
| CN106689825A (en) * | 2016-12-05 | 2017-05-24 | 长沙秋点兵信息科技有限公司 | High-hatchability feed for bactrocera mediterranei larvae |
| CN106954764A (en) * | 2017-03-17 | 2017-07-18 | 中国热带农业科学院环境与植物保护研究所 | A kind of artificial feed for melon fly larvae and preparation method thereof |
| CN110613065A (en) * | 2019-07-29 | 2019-12-27 | 海南大学 | Melon fly solid culture medium and preparation method thereof |
| CN115886160A (en) * | 2022-11-30 | 2023-04-04 | 华南农业大学 | Feed for larvae of Bactrocera dorsalis in south Asia |
| CN115886160B (en) * | 2022-11-30 | 2024-05-28 | 华南农业大学 | Nanya fruit fly larva feed |
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