CN107517934A - A kind of more worm state box for feeding pests of citrus fruit fly and its method for breeding - Google Patents
A kind of more worm state box for feeding pests of citrus fruit fly and its method for breeding Download PDFInfo
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- CN107517934A CN107517934A CN201710796067.4A CN201710796067A CN107517934A CN 107517934 A CN107517934 A CN 107517934A CN 201710796067 A CN201710796067 A CN 201710796067A CN 107517934 A CN107517934 A CN 107517934A
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- gauze
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- citrus fruit
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- 241000255588 Tephritidae Species 0.000 title claims abstract description 48
- 235000020971 citrus fruits Nutrition 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000009395 breeding Methods 0.000 title claims description 13
- 230000001488 breeding effect Effects 0.000 title claims description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 30
- 241000382353 Pupa Species 0.000 claims abstract description 27
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 26
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 26
- 210000004681 ovum Anatomy 0.000 claims abstract description 26
- 239000006004 Quartz sand Substances 0.000 claims abstract description 24
- 239000011521 glass Substances 0.000 claims abstract description 19
- 241000238631 Hexapoda Species 0.000 claims abstract description 14
- 230000018109 developmental process Effects 0.000 claims abstract description 10
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 10
- 239000010935 stainless steel Substances 0.000 claims abstract description 10
- 239000004459 forage Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000012447 hatching Effects 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 8
- 239000000284 extract Substances 0.000 claims description 7
- 235000004936 Bromus mango Nutrition 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 240000007228 Mangifera indica Species 0.000 claims description 6
- 235000014826 Mangifera indica Nutrition 0.000 claims description 6
- 235000009184 Spondias indica Nutrition 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 239000005667 attractant Substances 0.000 claims description 4
- 230000031902 chemoattractant activity Effects 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 3
- 241000209140 Triticum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 3
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims description 3
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims description 3
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims description 3
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- 230000032669 eclosion Effects 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 230000019617 pupation Effects 0.000 description 5
- 241000675108 Citrus tangerina Species 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
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- 230000003031 feeding effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000009368 gene silencing by RNA Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000003716 rejuvenation Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 244000241257 Cucumis melo Species 0.000 description 1
- 241000798443 Hornodermoporus martius Species 0.000 description 1
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- 238000002386 leaching Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feeding And Watering For Cattle Raising And Animal Husbandry (AREA)
Abstract
The present invention relates to a kind of cultured insect equipment, the more worm state box for feeding pests of particularly a kind of citrus fruit fly and its cultural method, box for feeding pests casing top side, bottom side and rear side use stainless steel gauze, and the left and right sides uses glass plate；Glass plate top sets through hole, and the feed pipe through glass plate, feed pipe upper opening are set in casing, and feed pipe one end setting diameter is more than the fixed cover of through hole, and the other end sets horizontal upward dog-house；On both sides of the middle sets support in casing, and material disc is placed on support；Cabinet front top uses gauze, and gauze center sets gauze to tuck inside the sleeve, and the magic tape that gauze is set by outer rim is connected with casing；Stainless steel gauze is used in the middle part of cabinet front, cabinet front bottom sets drawer type collet, quartz sand is laid in collet；Sponge is placed in feeding tube opening, gauze is covered outside sponge, facilitates trypetid imago feeding；The collet both ends of cabinet front set soft magnetic stripe, avoid trypetid from escaping；Also it is hung with below box for feeding pests inner bottom part and takes egg apparatus.The box for feeding pests can accurately obtain the experiment material of the specific developmental processes of four worm states such as the ovum of trypetid, larva, pupa, adult.
Description
Technical field
The present invention relates to a kind of cultured insect equipment, the more worm state box for feeding pests of particularly a kind of citrus fruit fly and its cultivation side
Method.
Background technology
Citrus fruit fly（Bactrocera dorsalis（Hendal））, also known as orient fruit fly (oriental fruit
Fly), Diptera is belonged to（Diptera）Tephritidae (Trypetidea), Bactrocera (Bactrocera), it is that the class of China two enters the territory
Quarantine pest.What the worm can endanger 40 sections more than 300 plants melon and fruit.Yunnan Province is one of generating region of citrus fruit fly, and the worm is tight
Yunnan Province's Perenniporia martius fruit, such as pomegranate, mango, banana, orange, orange, pears, guava various fruits etc. are endangered again,
Cause serious economic loss.For a long time, how effective prevention and control citrus fruit fly turns into the focus studied both at home and abroad, establishes stable
Experimental population be the key for studying the effective Prevention Technique of the worm and method.In recent years, with the development of insect molecular biology,
By the method for insect genes groups, using gene perturbation technique, such as RNAi technology, to control pest damage into the heat of research
One of point.RNAi etc. prevents and treats the research and development of new technology, and higher requirement is proposed to insect experimental population, for citrus fruit fly,
Substantial amounts of citrus fruit fly test material is not only needed, and requires accurately obtain different days not at any time during experiment
With the test material of worm state, traditional citrus fruit fly breeding apparatus, it is difficult to meet the requirement of above-mentioned insect test material.
Prior art（Pan Fei, Qin Shuan, Xiao Tongbin, a kind of simple breeding technology [J] plant protection of melonflies is waited,
2017,6:62-63.）Devise a kind of simple insect cage（100cm×45cm×120cm）Melonfly is raised, its insect cage is overall
For stainless steel, both sides are iron sand net, and cage top portion, front portion, rear portion and bottom are tumbled in lucite, but with insectary ring
The effect that border is consistent is barely satisfactory.Another prior art（Yuan Shengyong, Kong Qiong, Xiao Chun citrus fruit fly adult man-made feeds
Screen [J] Hua Zhong Agriculture University journal）Insect cage, cage top portion, front-back, two sides tumble in glass, are only opened on glass
Individual diameter 14cm circular port, equally it is difficult to ensure that in case that environment is consistent with insectary environmental condition.
The content of the invention
It is difficult that satisfaction can accurately obtain different days difference worm state insect at any time for traditional citrus fruit fly breeding apparatus
The problem of test material, the present invention propose a kind of more worm state box for feeding pests of citrus fruit fly.
The more worm state box for feeding pests of citrus fruit fly of the present invention, using the rectangular box with leg, it is characterised in that casing top side,
Bottom side and rear side use stainless steel gauze, and the left and right sides uses glass plate；Glass plate top sets through hole, sets and passes through in casing
The feed pipe of glass plate, feed pipe upper opening are worn, feed pipe one end sets diameter to be set more than the fixed cover of through hole, the other end
Horizontal upward dog-house；On both sides of the middle sets support in casing, and material disc is placed on support；Cabinet front top uses yarn
Cloth, gauze center set gauze to tuck inside the sleeve, and the magic tape that gauze is set by outer rim is connected with casing；Using stainless in the middle part of cabinet front
Steel gauze, cabinet front bottom set drawer type collet, quartz sand are laid in collet；Sponge, sponge are placed in feeding tube opening
Outer covering gauze, facilitates trypetid imago feeding；The collet both ends of cabinet front set soft magnetic stripe, avoid trypetid from escaping；In foster worm
Also it is hung with below case inner bottom part and takes egg apparatus, described to go egg apparatus to be connected through a screw thread by two hollow hemispheres to form, outward appearance is in shallow
Yellow green, several spawning apertures are uniformly distributed above；Circular hole is provided with the top of the hollow hemisphere of top, running through in circular hole has one carefully
Bar, thin bar fineness are consistent with episphere circular hole size, and thin bar bottom is provided with the container for holding attractant, and thin bar is leaned on
Circular stull is provided with below nearly aperture, the hook cap of threaded connection is provided with the top of thin bar, hook cap bottom is vertically set
The screw for coordinating thin bar to use is put, horizontal hole is arranged at top.
Described quartz sand must sterilize and keep moistening, i.e., pinching anhydrous ooze of quartz sand with hand shows, and hand is decontroled can nature
Scatter.
The box for feeding pests adds material disc, feed pipe design, conveniently feeds and cleans, trypetid larva is positioned in material disc
Facilitate the bait of feeding material disc, jump into collet and pupate when larval growth is developed to mature larva, quartz sand has buffering to make
With；Trypetid adult is active in casing internal upper part, passes through feed pipe feeding.Glass plate is easy to researcher to observe citrus fruit fly worm state
Change.Relatively low humidity is needed when relatively high humidity is needed in larva worm state additionally, due to trypetid, and being pupated, quartz sand
Easily sterilizing, quartz sand avoid going mouldy when trypetid can be made to pupate.Box for feeding pests is except two sides glass plate support feeding tube
Outside, its lap is all made up of iron sand net and gauze, and leg can be such that bottom isolates with ground, is easy to the circulation of air, can be very
Environment is consistent with insectary environment in good guarantee box for feeding pests, and the condition such as temperature, humidity and illumination by controlling insectary is just
Environment in the case that citrus fruit fly grows can be ensured.Take egg apparatus that citrus fruit fly can be lured to produce ovum at it through spawning aperture
Inner surface, the ovum of inner surface is brushed down with writing brush or brush, is sprinkled upon in material disc and breeds., can be by worm's ovum by taking egg apparatus
Centralized collection, developmental process and the time of ovum can be accurately grasped, is equably dispersed in the larvae feeds of material disc, makes citrus fruit fly
It is larva growing homogenous, energy accurate perception larvae development process and time, convenient that resource optimization is carried out to raising resource.
Using the method for breeding of the box for feeding pests, it is positioned in insectary, the adult that contemporaneity is sprouted wings is gathered and supported above-mentioned
In box for feeding pests, adult forage is added in feed pipe within every 24 hours, larvae feeds are added in material disc, two to three days to above-mentioned
Box for feeding pests is cleaned and sterilization；The worm's ovum centralized collection that will be taken daily in egg apparatus, equably it is dispersed in the larva of material disc
In feed, and record adopts the details of ovum in detail, and the ovum of different days can be obtained before egg hatching, can be accurate after egg hatching
Grasp the developmental process of larva（Such as：Age in days）, raise to there is mature larva to jump into the quartz sand same day, extract out daily and change bottom
Support, sifts out the pupa changed in collet after 24 hours, is placed in the quartz sand for mix up humidity and preserves, successively multi-cycle separation, and carry out
The details pupated, it can accurately grasp the developmental process of pupa（Such as：Age in days）, different days can be obtained before pupa emergence
Pupa；Raised to the citrus fruit fly adult same day for finding to have emergence, extract out daily and change collet, firm emerging adult in collet is small
The heart, which is picked up, to be put back in original rearing-box, and the collet equipped with pupa of not sprouting wings is put into the more worm state box for feeding pests of standby citrus fruit fly
In, multi-cycle separation, untill being put into the pupa sprouted wings in case and all sprouting wings, so then separates the adult to sprout wings daily successively,
The developmental process of adult can accurately be grasped（Such as：Age in days）, so as to conveniently get the adult of different days as needed.
Described adult forage uses mango juice, or yeast extract and sucrose to press 1:The aqueous solution made from 3 ratio.Adult
Feed uses liquid, and adult liquid feed, the outer covering yarn for the sterilization being put into pipe are poured into by the dog-house of feed pipe both sides
The sponge strip of cloth, feed pipe can slowly release feed liquid, facilitate imago feeding, and both ends are screwed the convenient supplement of lid or more
Feed is changed, can also avoid citrus fruit fly from escaping.Described larvae feeds are using bean dregs 45.0g, wheat germ 7g, sucrose 14g, yeast
Powder 7g, hydrochloric acid 0.3ml, methyl p-hydroxybenzoate 0.3g are subject to appropriate distilled water and are configured to mass, by its feed
PH value is regulated to 3.6.Such a feed is exclusively used in eating fly larvae, and feeding effect is obvious.
As another embodiment of this box for feeding pests, the fruit such as the mango thinly sliced or banana are only launched in material disc
Adult forage and larvae feeds are used as simultaneously, be built into clear water in feed pipe drinks water for citrus fruit fly adult, adds water every time to sea
It is continuous all to soak.
Described insectary requires ventilation and penetrating light, is avoided that the interference of other insects or animal；Insectary is equipped with artificial light
Source, indoor humidifier adjust humidity, and holding relative humidity is 50%-60%, and for temperature at 25-30 DEG C, light dark period is light：Secretly=
12h：12h.
The more worm state box for feeding pests of trypetid of the present invention, it can not only meet that natural fruit raises trypetid, can also meet man-made feeds
The requirement of extensive raising trypetid, it is crucial that, using this new box for feeding pests and its method for breeding, it can accurately obtain reality
The specific developmental process of four worm states such as the ovum of fly, larva, pupa, adult（Age in days）Experiment material, and feeding, insect-taking, more
The operating process changed and cleaned etc. is simple and convenient, and device cost of manufacture is cheap.The box for feeding pests and its method for breeding, can be that tangerine is small
Trypetid and other fruit flies（Bactrocera）The research of insect provides more, finer test material.And traditional box for feeding pests
The acquisition of different days, different worm state test materials is not accomplished to be accurate to.In addition, the box for feeding pests and its method for breeding are also convenient for trying
Test the rejuvenation of insect, it is only necessary to be put into from the aggrieved fruit of field acquisition in case, treat that mature larva is jumped into collet, but be transferred to other
Raised in casing, that is, complete the rejuvenation of experiment insect.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention.
Wherein, feed pipe 1, material disc 2, gauze sleeve 3, collet 4.
Embodiment
Embodiment 1：The present embodiment picks up from Yunnan using the man-made feeds raising small food fly of tangerine, raising worm sources citrus fruit fly larva
The blue or green jujube garden wormed fruit of province Salvador in Yuanjiang County of Yunnan Province plant protection unit, citrus fruit fly larva is put into the more worm state box for feeding pests of citrus fruit fly, the box for feeding pests is adopted
With the rectangular box with leg, it is characterised in that casing top side, bottom side and rear side use stainless steel gauze, and the left and right sides uses
Glass plate；Glass plate top sets through hole, and the feed pipe through glass plate, feed pipe upper opening, feed pipe are set in casing
One end sets diameter, and more than the fixed cover of through hole, the other end sets horizontal upward dog-house；On both sides of the middle sets branch in casing
Frame, material disc is placed on support；Cabinet front top uses gauze, and gauze center sets gauze to tuck inside the sleeve, and gauze is set by outer rim
Magic tape be connected with casing；Stainless steel gauze is used in the middle part of cabinet front, cabinet front bottom sets drawer type collet, collet
Interior laying quartz sand；Sponge is placed in feeding tube opening, gauze is covered outside sponge, facilitates trypetid imago feeding；Cabinet front
Collet both ends set soft magnetic stripe, avoid trypetid from escaping；Also be hung with below box for feeding pests inner bottom part and take egg apparatus, it is described go egg apparatus by
Two hollow hemispheres are connected through a screw thread and formed, and outward appearance is in chartreuse, are uniformly distributed several spawning apertures above；Top is empty
Circular hole is provided with the top of heart hemisphere, running through in circular hole has a thin bar, and thin bar fineness is consistent with episphere circular hole size, thin bar
Bottom is provided with the container for holding oviposition attractant, and thin bar is provided with circular stull below aperture, set at the top of thin bar
The hook cap being connected through a screw thread is equipped with, hook cap bottom is vertically arranged the screw for coordinating thin bar to use, and horizontal hole is arranged at top.
Quartz sand must sterilize and keep moistening, i.e., pinching anhydrous ooze of quartz sand with hand shows, and hand is decontroled meeting nature and scattered.Utilize this
The method for breeding of box for feeding pests, is positioned in insectary, and the adult that contemporaneity is sprouted wings is gathered and supported in above-mentioned box for feeding pests, every 24 is small
When adult forage is added in feed pipe, larvae feeds are added in material disc, above-mentioned box for feeding pests were cleaned in two to three days
And sterilization；The worm's ovum centralized collection that will be taken daily in egg apparatus, is equably dispersed in the larvae feeds of material disc, and remember in detail
The details of ovum are adopted in record, and the ovum of different days can be obtained before egg hatching；The development of larva can be accurately grasped after egg hatching
Process（Age in days）, raise to there is mature larva to jump into the quartz sand same day, extract out daily and change collet, collet is sifted out after 24 hours
The pupa inside changed, is placed in the quartz sand for mix up humidity and preserves, successively multi-cycle separation, and carries out the details pupated, in pupa
The pupa of different days can be obtained before emergence；Raised to the citrus fruit fly adult same day for finding to have emergence, extract out and change daily
Collet, firm emerging adult in collet is carefully picked up and put back in original rearing-box, the collet equipped with pupa of not sprouting wings is put into standby
In the more worm state box for feeding pests of citrus fruit fly, multi-cycle separation successively, untill being put into the pupa sprouted wings in case and all sprouting wings, so
Then the adult to sprout wings daily is separated, and obtains the adult of different days.Described adult forage uses mango juice, or yeast leaching
Powder and sucrose press 1:The aqueous solution made from 3 ratio.Adult forage uses liquid, coordinates and covers gauze outside sponge and sponge, into
Worm feeding, while by the timely supplement feed of dog-house of feed pipe both sides, citrus fruit fly can be avoided to escape, and pass through fixed cover
Liquid is released, regularly replaces adult forage；Described larvae feeds are using bean dregs 45.0g, wheat germ 7g, sucrose 14g, dusty yeast
7g, hydrochloric acid 0.3ml, methyl p-hydroxybenzoate 0.3g are subject to appropriate distilled water and are configured to mass, by the PH of its feed
Value is regulated to 3.6.Such a feed is exclusively used in eating fly larvae, and feeding effect is obvious.
Embodiment 2：The present embodiment picks up from Yunnan Province's member using the fruit raising small food fly of tangerine, raising worm sources citrus fruit fly larva
The blue or green jujube garden wormed fruit of river county plant protection unit, citrus fruit fly larva is put into the more worm state box for feeding pests of citrus fruit fly, the box for feeding pests uses band
The rectangular box of leg, it is characterised in that casing top side, bottom side and rear side use stainless steel gauze, and the left and right sides uses glass
Plate；Glass plate top sets through hole, and the feed pipe through glass plate, feed pipe upper opening, feed pipe one end are set in casing
Diameter is set to be more than the fixed cover of through hole, the other end sets horizontal upward dog-house；On both sides of the middle sets support, branch in casing
Material disc is placed on frame；Cabinet front top uses gauze, and gauze center sets gauze to tuck inside the sleeve, the thread gluing that gauze is set by outer rim
Band is connected with casing；Stainless steel gauze is used in the middle part of cabinet front, cabinet front bottom sets drawer type collet, laid in collet
Quartz sand；Sponge is placed in feeding tube opening, gauze is covered outside sponge, facilitates trypetid imago feeding；The collet two of cabinet front
End sets soft magnetic stripe, avoids trypetid from escaping；Also it is hung with below box for feeding pests inner bottom part and takes egg apparatus, it is described to remove egg apparatus by two skies
Heart hemisphere, which is connected through a screw thread, to be formed, and outward appearance is in chartreuse, is uniformly distributed several spawning apertures above；Top hollow hemisphere
Top is provided with circular hole, and running through in circular hole has a thin bar, and thin bar fineness is consistent with episphere circular hole size, and thin bar bottom is set
The container for holding attractant is equipped with, thin bar is provided with circular stull below aperture, spiral shell is provided with the top of thin bar
The hook cap of line connection, hook cap bottom are vertically arranged the screw for coordinating thin bar to use, and horizontal hole is arranged at top.Quartz sand is necessary
Sterilize and keep moistening, i.e., pinching anhydrous ooze of quartz sand with hand shows, and hand is decontroled meeting nature and scattered.Utilize the feeding of the box for feeding pests
The method of supporting, is positioned in insectary, and the adult that contemporaneity is sprouted wings is gathered and supported in above-mentioned box for feeding pests, every 24 hours in material disc
Interior addition feed, is cleaned and sterilization for two to three days to above-mentioned box for feeding pests；The worm's ovum taken in egg apparatus is concentrated daily and received
Collection, is equably dispersed in the larvae feeds of material disc, and record adopts the details of ovum in detail, can be obtained not before egg hatching
With the ovum of age in days；Raising is extracted out to there is mature larva to jump into the quartz sand same day and changes collet, collet is sifted out after 24 hours daily
The pupa inside changed, is placed in the quartz sand for mix up humidity and preserves, successively multi-cycle separation, and carries out the details pupated, in pupa
The pupa of different days can be obtained before emergence；Raised to the citrus fruit fly adult same day for finding to have emergence, extract out and change daily
Collet, firm emerging adult in collet is carefully picked up and put back in original rearing-box, the collet equipped with pupa of not sprouting wings is put into standby
In the more worm state box for feeding pests of citrus fruit fly, multi-cycle separation successively, untill being put into the pupa sprouted wings in case and all sprouting wings, so
Then the adult to sprout wings daily is separated, and obtains the adult of different days.The mango or banana thinly sliced are launched in material disc
Adult forage and larvae feeds are used as simultaneously Deng fruit, be built into clear water in feed pipe drinks water for citrus fruit fly adult, adds every time
Water all soaks to sponge.
Taken 500 ovum are placed in the artificial larvae feeds configured, allow it freely to hatch, record and statistics ovum are incubated
Change the quantity of the larva of gained, so as to obtain the incubation rate of ovum（Incubation rate=hatch larva number/experiment ovum number）；It will take at random
300 larvas gone out, which are put into artificial beverage, to be raised, and is treated that it is developed to mature larva and jumped into sand and is pupated, collect pupa daily, remembers
Record and the quantity of statistics pupa（Percentage of pupation=number/experiment larva number of pupating）；200 pupas of random taking-up are put into bottom drawer formula support
In the fine sand of disk（And do not have when keeping the humidity of fine sand to be clutched with hand under water droplet, unclamp hand sand and scatter naturally）Allow its free plumage
Change, the quantity of adult after statistics is sprouted wings（Eclosion rate=emerging adult number/experiment pupa number）.
Experimental result is shown in Table 1.
Two kinds of different box for feeding pests are compared by upper table and raise citrus fruit fly, incubation rate, percentage of pupation and the eclosion rate of ovum etc.
Biological indicator, result of the test can be seen that the artificial feeding that citrus fruit fly is carried out using the more worm state box for feeding pests of new trypetid, ovum
Incubation rate 95.7%, percentage of pupation 95.1%, eclosion rate 94.63%.The artificial feeding of citrus fruit fly is carried out using traditional box for feeding pests,
Incubation rate 79.5%, percentage of pupation 85.6%, the eclosion rate 79.5% of ovum.It can be seen that the hatching of the ovum of box for feeding pests raising citrus fruit fly
Rate, percentage of pupation, eclosion rate are substantially higher than the raising of traditional box for feeding pests.Illustrate to use the box for feeding pests and the small reality of method indoor feeding tangerine
Fly effect is good, is feasible.
Claims (5)
1. the more worm state box for feeding pests of citrus fruit fly, using the rectangular box with leg, it is characterised in that casing top side, bottom side and after
Side uses stainless steel gauze, and the left and right sides uses glass plate；Glass plate top sets through hole, is set in casing through glass plate
Feed pipe, feed pipe upper opening, feed pipe one end set diameter to be more than the fixed cover of through hole, and the other end sets horizontal upward
Dog-house；On both sides of the middle sets support in casing, and material disc is placed on support；Cabinet front top uses gauze, gauze center
Gauze is set to tuck inside the sleeve, the magic tape that gauze is set by outer rim is connected with casing；Stainless steel gauze, casing are used in the middle part of cabinet front
Lower front sets drawer type collet, and quartz sand is laid in collet；Sponge is placed in feeding tube opening, gauze is covered outside sponge,
Facilitate trypetid imago feeding；The collet both ends of cabinet front set soft magnetic stripe, avoid trypetid from escaping；Below box for feeding pests inner bottom part
Also be hung with and take egg apparatus, described to take egg apparatus to be connected through a screw thread by two hollow hemispheres to form, outward appearance is in chartreuse, above
Several spawning apertures of even distribution；Circular hole is provided with the top of the hollow hemisphere of top, running through in circular hole has a thin bar, thin bar thickness journey
Degree is consistent with episphere circular hole size, and thin bar bottom is provided with the container for holding attractant, and thin bar is set below aperture
Circular stull is equipped with, the hook cap of threaded connection is provided with the top of thin bar, hook cap bottom, which is vertically arranged, coordinates thin bar to make
Horizontal hole is arranged at screw, top.
2. the more worm state box for feeding pests of citrus fruit fly as claimed in claim 1, it is characterised in that quartz sand must sterilize and keep wet
Profit, i.e., pinch anhydrous ooze of quartz sand with hand and show, hand is decontroled meeting nature and scattered.
3. utilize the citrus fruit fly method for breeding of more worm state box for feeding pests, it is characterised in that be positioned in insectary, by contemporaneity
The adult of emergence is poly- to be supported in above-mentioned box for feeding pests, adult forage is added in feed pipe within every 24 hours, children is added in material disc
Worm feed, is cleaned and sterilization for two to three days to above-mentioned box for feeding pests；The worm's ovum centralized collection that will be taken daily in egg apparatus,
It is dispersed in evenly in the larvae feeds of material disc, and record adopts the details of ovum in detail, can be obtained not on the same day before egg hatching
The ovum in age；The developmental process of larva can be accurately grasped after egg hatching（Age in days）, raising to there is mature larva to jump into the quartz sand same day,
Extract out daily and change collet, the pupa changed in collet is sifted out after 24 hours, is placed in the quartz sand for mix up humidity and preserves, successively
Multi-cycle separation, and the details pupated are carried out, the pupa of different days can be obtained before pupa emergence；Raising to discovery has emergence
The citrus fruit fly adult same day, extract out daily and change collet, firm emerging adult in collet is carefully picked up and puts back to original raising
In case, the collet equipped with pupa of not sprouting wings is put into the more worm state box for feeding pests of standby citrus fruit fly, successively multi-cycle separation, until
Untill being put into the pupa whole emergence sprouted wings in case, so then the adult to sprout wings daily is separated, and obtain the adult of different days.
4. the citrus fruit fly method for breeding of more worm state box for feeding pests as claimed in claim 3, it is characterised in that adult forage uses
Mango juice, or yeast extract and sucrose press 1:The aqueous solution made from 3 ratio；Larvae feeds using bean dregs 45.0g, wheat germ 7g,
Sucrose 14g, dusty yeast 7g, hydrochloric acid 0.3ml, methyl p-hydroxybenzoate 0.3g are subject to appropriate distilled water and are configured to pasty state feeding
Material, 3.6 are regulated to by the pH value of its feed.
5. the citrus fruit fly method for breeding of more worm state box for feeding pests as claimed in claim 3, it is characterised in that insectary requires logical
Wind printing opacity, it is avoided that the interference of other insects or animal；Insectary is equipped with artificial light source, and indoor humidifier adjusts humidity, protects
It is 50%-60% to hold relative humidity, and for temperature at 25-30 DEG C, light dark period is light：Secretly=12h：12h.
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