CN109496990A - A kind of device and method for indoor raising drosophila parasitic wasp - Google Patents
A kind of device and method for indoor raising drosophila parasitic wasp Download PDFInfo
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- CN109496990A CN109496990A CN201811308993.3A CN201811308993A CN109496990A CN 109496990 A CN109496990 A CN 109496990A CN 201811308993 A CN201811308993 A CN 201811308993A CN 109496990 A CN109496990 A CN 109496990A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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Abstract
The invention belongs to plant protection arts and technical field of insect feeding, and in particular to a kind of device for indoor raising drosophila parasitic wasp, and further disclose a kind of method for carrying out indoor raising drosophila parasitic wasp using the device.Device of the present invention for indoor raising drosophila parasitic wasp, process by simulating fruit shapes and parasitic wasp host in raising container is designed, and the method for indoor raising drosophila parasitic wasp of the present invention, the raising of parasitic wasp is carried out using the device, the parasitic rate and bee yield of parasitic wasp are effectively increased, to meet the prevention and treatment demand of melon and fruit field of planting pest.
Description
Technical field
The invention belongs to plant protection arts and technical field of insect feeding, and in particular to one kind is for indoor raising drosophila
The device of parasitic wasp, and further disclose a kind of method for carrying out indoor raising drosophila parasitic wasp using the device.
Background technique
Drosophila feeding habits are extensive, and growing environment is special, like dark moist habitat, have to sour-sweet and corrupt smell bright
Aobvious taxis, the succulence organ of can cause harm a variety of gourd, fruit and vegetables and many plants are important agricultural pests.Studies have shown that in indigo plant
The cultivation of a variety of berries such as the certain kind of berries, blackberry, blueberry, cherry, strawberry, plum, peach, grape, fig, Kiwi berry and pears and stone fruit
During training, the harm of spotted wing drosophila and Drosophila melanogaster be can not be ignored.For example, spotted wing drosophila can lay eggs on new fresh grape,
Fruit caused by feeding softens after mechanical damage and larvae hatch caused by oviposition, can cause rotting for grape, breeds big
Microorganism is measured, and causes largely laying eggs, seriously affect the yield and quality of fruit for Drosophila melanogaster, or even cause to have no harvest.Therefore,
Have great importance to the preventing and controlling of drosophila for the plantation of melon and fruit.And as people's environmental consciousness and integrated control are realized
Enhancing, simple pesticide control gradually appears its harm, and carries out biological control using natural enemy and increasingly received
With use.Therefore, kind of a team control is carried out to drosophila class pest using natural enemy insect more and more in integrated pest control management
System.
Hair angle clavicorn serphid Trichopria drosophilae Perkins, belongs to Hymenoptera Diapriidae, is drosophila
The main parasitic bee of mature larva, host range is wide, if carrying out mass propagation and being discharged, can have to the prevention and treatment of drosophila
Apparent control action.Studies have shown that development is higher from the hair angle clavicorn serphid eclosion rate of Drosophila melanogaster pupa and parasitic efficiency, and
And advantage long with the service life, that fertility is strong, it is the good source in terms of preventing and treating drosophila.If can vast propagation hair angle clavicorn
Serphid has important meaning for the effect for improving control of insect in actual production.
But currently, both at home and abroad about the raising of drosophila parasitic wasp there are technical obstacle, in conventional husbandry methods, due to
The uncontrollability of humidity and the deterioration of feeding environment, cause the parasitic wasp death rate higher, and therefore, there is no develop in the prior art
More effectively it is suitable for the method for indoor mass rearing parasitic wasp out.Therefore, it develops one kind and is suitable for indoor raising drosophila
The device and method for breeding of parasitic wasp plant pest for improving the parasitic rate and bee yield of drosophila parasitic wasp, and to melon and fruit
Prevention and treatment have great importance.
Summary of the invention
For this purpose, technical problem to be solved by the present invention lies in provide a kind of dress for indoor raising drosophila parasitic wasp
It sets, to solve the problems, such as that the indoor raising drosophila parasitic wasp death rate is higher in the prior art；
Second technical problem to be solved by this invention is to provide a kind of utilization above-mentioned apparatus raising drosophila parasitic wasp
Method, this method is host with Drosophila melanogaster, mix by simulation fruit shapes and with feed media, and utilize suspension type
The method of raising is raised.So that the parasitic rate of parasitic wasp, bee yield are enhanced, for for the use for trying parasitic wasp
And large-scale production provides advantage.
In order to solve the above technical problems, a kind of device for indoor raising drosophila parasitic wasp of the present invention, including
For the raising container of parasitic wasp breeding, the parasitic agent for being suitable for parasitic wasp breeding is vacantly provided in the raising container；
The parasitic agent is the Agar substrate for being mixed with the ball shape structure of drosophila host and host's feed.
The parasitic agent includes agar ball and is coated on be mixed with drosophila host and the host of the agar ectosphere and raises
The media packs layer of material.
The agar ball is preferably diameter 6-8cm, and the thickness of the media packs layer is preferably 1-2cm, i.e., described to post
The diameter of raw medium is preferably 8-10cm, this thickness had not only guaranteed the humidity of man-made feeds, but also can prevent due to the excessive humidity of agar
It is excessive that man-made feeds is caused to go mouldy.
The parasitic agent is contained in scalability mesh bag, and is hung in the raising container.
Gauze used in the scalability mesh bag is common hospital gauze, at low cost, is easily obtained.Compare nylon yarn
For cloth, flexibility is stronger, can tightly wrap up simulation fruit, drill out and pupate convenient for drosophila larvae, be also convenient for parasitic wasp parasitism,
Improve parasitic rate.
The drosophila host is the mature larva of Drosophila melanogaster.
When using Drosophila melanogaster larva as host, the age selected is mature larva, according to previous experiments as a result, it has been found that,
The best host insect state of parasitic wasp is the intermediate stage between mature larva and pupa, and this stage is shorter, average time 0.5
It, therefore using mature larva as host condition, parasitic efficiency can be improved.
Host's feed includes the component of following parts by weight: banana 100-130 parts by weight, apple 100-130 parts by weight,
Banana corn flour 40-50 parts by weight, sucrose 20-25 parts by weight, yeast powder 20-25 parts by weight, preservative 1-2 parts by weight and
Agar 8-10 parts by weight.
The preservative is preferably food preservative well known in the prior art, such as is preferably potassium sorbate.
The present invention also provides a kind of methods of indoor raising drosophila parasitic wasp, including parasitic wasp to be placed in the device
The step of middle progress expanding propagation.
Specifically, the method for the indoor raising drosophila parasitic wasp, includes the following steps:
(1) field acquisition Drosophila melanogaster larva and pupa, and artificial feeding to its sprout wings parasitic wasp out after as parasitic wasp worm
Kind is numerous wait expand, spare；
(2) host's feed is made according to selected components and ratio, and carries out the drosophila using host's feed
The raising of host, and the host's feed for being mixed with the drosophila host is coated on agar ectosphere, parasitic Jie is made
Matter is simultaneously hung in the raising container；
(3) parasitic wasp obtained in step (1) is placed in the raising container and expand numerous raising, parasitic wasp oviposition knot
Shu Houhui is dead；
(4) parasitic wasp was raised to after 15-20 days, the parasitic wasp out that sprouts wings is transferred in new raising container into
Row expands numerous.
In the step (1), the condition of the artificial feeding are as follows: 26 ± 1 DEG C of raising temperature of control, relative humidity 50-
60%, light application time 16L:8D.
In the step (2), the preparation of the parasitic agent is specifically included:
Weigh a selected amount of banana and apple, with being made pureed after mixing, and with a selected amount of banana corn
Powder, sucrose, yeast powder, preservative and agar mix, and are placed in pallet and are cooled to semisolid,；
The Drosophila melanogaster adult that obtained parasitic feed semisolid is placed in cage, and discharges egg production peak time carries out numerous
It grows；After Drosophila melanogaster oviposition for 24 hours, it is old to its development that taking-up pallet, which is placed in the insectary of the same terms, which breeds 3-4 days,
Ripe larva is to get to the parasitic forage mixture for being mixed with required drosophila host；
The mixture of the resulting parasitic feed for being mixed with drosophila host is coated on agar ball surface, ball shape structure is made
Required parasitic agent.
The process of above-mentioned raising Drosophila melanogaster mature larva only allows Adult worms producting eggs for 24 hours, this is because Drosophila melanogaster produces
It when ovum peak period, lays eggs 30-40 per female within 24 hours, is convenient for the larva quantity in rough Statistics pallet after egg hatching as a result,
The quantity of placement Drosophila melanogaster adult can be determined according to the demand of larva.
Pallet thickness used in the man-made feeds of above-mentioned raising drosophila is no more than 1cm, it is ensured that in package agar ball, production
When simulating the parasitic agent of fruit shape, the larva of Drosophila melanogaster is convenient for parasitic wasp parasitic in the outer layer of " fruit ".
In the step (3), the supply volume ratio of the Drosophila melanogaster and the parasitic wasp is 40-60:1-1.5, and is controlled
The male and female quantitative proportion for making the parasitic wasp launched is 1-3:1, during the feeding process, by the above-mentioned parameter ratio amount of progress
Change, population colonization speed can be effectively improved.
Device of the present invention for indoor raising drosophila parasitic wasp, by the simulation fruit shapes in raising container and
The process of parasitic wasp host is designed, and the Agar substrate for being mixed with parasitic drosophila and parasitic feed is vacantly set to the raising
In container, with the life habit of analog parasitic bee, the parasitic rate and bee yield of parasitic wasp are effectively increased；And passing through will be described
Parasitic agent is designed as ball shape structure, increases effectively area and probability that parasitic wasp finds host, further improves parasitism
The parasitic rate of bee.Also, breeding apparatus material therefor cost of the present invention is lower, easily obtains, easy to operate, to raising people
Member is suitable for promoting on a large scale without very high technical requirements.
The method of raising drosophila parasitic wasp in interior of the present invention, is practised using the life of above-mentioned breeding apparatus analog parasitic bee
Property, and the control of the quantity of Drosophila melanogaster larva and parasitic wasp can be reduced the waste of insect resources, not only grasped in certain proportion
Make simply, it is at low cost, raising scale is effectively improved, the parasitic rate of parasitic wasp is improved, improves answering in insect raising field
With value.
Detailed description of the invention
In order to make the content of the present invention more clearly understood, it below according to specific embodiments of the present invention and combines
Attached drawing, the present invention is described in further detail, wherein
Fig. 1 is the structural schematic diagram of raising drosophila parasitic wasp device of the present invention；
Fig. 2 is the structural schematic diagram of scalability mesh bag of the present invention；Wherein, A, B respectively indicate the mesh bag in Fig. 2
Structural form before and after being packed into the parasitic agent；
Appended drawing reference indicates in figure are as follows: 1- raises container, 2- mesh bag, 3- parasitic agent, 4- media packs layer, 5- agar
Ball, 6- square, 7- gauze, 8- drawstring, 9- hang iron wire, 10- filter paper.
Specific embodiment
Apparatus structure as shown in Figure 1, the device of the present invention for indoor raising drosophila parasitic wasp, including raising are held
The open end of device 1, the raising container 1 is provided with square 6, and the hole of the square 6 is so that drosophila host and parasitic wasp are equal
The raising container that can not fly out is advisable.
In structure as shown in Figure 1, the top of the raising container 1 is additionally provided with suspension iron wire 9, the suspension iron wire 9
Place's suspension is provided with parasitic agent 3, and the parasitic agent 3 is the agar base for being mixed with the ball shape structure of drosophila host and host's feed
Matter, expansion for the parasitic wasp is numerous to be used.In breeding apparatus of the present invention, the parasitic agent 3 is contained in scalability
In mesh bag 2, the hanging setting of the parasitic agent 3 is realized.In the structure of mesh bag 2 as shown in Figure 2, the mesh bag 2 includes shape
At the gauze 7 of the splendid attire parasitic agent 3, and it is provided with drawstring 8 at the closing in position of the mesh bag 2, on the one hand for real
Closing in after the existing loading of parasitic agent 3, hangs on the suspension iron wire 9 simultaneously also by by the drawstring 8, realizes institute
State the hanging setting of parasitic agent 3.
And gauze 7 used in scalability mesh bag 2 of the present invention is common hospital gauze, compare nylon
For gauze, flexibility is stronger, can tightly wrap up simulation fruit, drill out and pupate convenient for drosophila larvae, be also convenient for parasitic wasp and post
It is raw, improve parasitic rate.
In structure as shown in Figure 1, the parasitic agent 3 is ball shape structure, and internal layer is agar ball 5, the agar ball 5
External sheath have the media packs layer 4 for being mixed with drosophila host and host's feed.The agar ball is preferably diameter 6-8cm, and
The thickness of the media packs layer is preferably 1-2cm, i.e., the diameter of the described parasitic agent is preferably 8-10cm, this thickness both guaranteed
The humidity of man-made feeds, and can prevent from causing man-made feeds to go mouldy since the excessive humidity of agar is excessive.
In the parasitic agent 3, the drosophila host is preferably the mature larva of Drosophila melanogaster, and host's feed is preferred
For the feed for being suitable for drosophila parasitic wasp breeding in the prior art, and form by addition agar the medium packet of solidifiable
Coating 4.In breeding apparatus of the present invention, host's feed preferably includes the component of following parts by weight: banana 100-130 weight
Measure part, apple 100-130 parts by weight, banana corn flour 40-50 parts by weight, sucrose 20-25 parts by weight, yeast powder 20-25 weight
Part, preservative 1-2 parts by weight and agar 8-10 parts by weight.
In breeding apparatus of the present invention, the preparation process of the parasitic agent 3 specifically may include following steps:
Banana 100-130 parts by weight, apple 100-130 parts by weight are weighed, pureed is made with juice extractor after being mixed；Separately
Row weighs banana corn flour 40-50 parts by weight, sucrose 20-25 parts by weight, yeast powder 20-25 parts by weight, preservative 1-2 weight
Part and agar 8-10 parts by weight, it is spare after being mixed with the banana puree and apple butter prepared；
The above-mentioned parasitic feed prepared is layered in the pallet of 30-50cm wide, 50-80cm long, control thickness is not higher than
1cm, it is to be cooled to be placed in cage of corresponding size as after semisolid, and the mesh number for controlling cage mesh enclosure is 60 mesh, is prevented
Drosophila escape；The then Drosophila melanogaster of release egg production peak time, after Drosophila melanogaster oviposition for 24 hours, taking-up pallet is placed in identical
It is mature larva to its development after 3-4 days in the insectary of condition, obtains the mixing for being mixed with parasitic feed and parasitic drosophila
Matrix；
Taking diameter is the agar ball 5 of 6-8cm, and utilizes spherical die, is mixed with parasitic feed and parasitic drosophila for resulting
Mixed-matrix be coated on the agar ball surface and form the media packs layer 4, control the media packs layer 4 with a thickness of
2cm or so obtains required ball-type parasitic agent 3；
The parasitic agent obtained is fitted into scalable mesh bag, is hung on the raising container 1 after tensing drawstring 8
On the suspension iron wire 9 of side, and a filter paper 10 is placed at the bottom of the raising container 1.
In the method for raising drosophila parasitic wasp in interior described in the following each embodiments of the present invention, it is all made of above-mentioned such as Fig. 1-2
The breeding apparatus of shown structure carries out.
Embodiment 1
The method of interior raising drosophila parasitic wasp described in the present embodiment, the breeding apparatus based on structure shown in Fig. 1-2 into
Row, specifically comprises the following steps:
(1) field acquisition Drosophila melanogaster larva and pupa, are placed in cage in the insectary of suitable condition, to its emergence
Out as the numerous source of expansion after parasitic wasp；Control the condition of the artificial feeding are as follows: 26 ± 1 DEG C of raising temperature, relative humidity 50-
60%, light application time 16L:8D；
(2) man-made feeds are prepared: weighing banana 120g, apple 120g, pureed is made with juice extractor after being mixed；Separately
Banana corn flour 45g, sucrose 22g, yeast powder 22g, preservative (potassium sorbate) 1.5g, agar 9g are weighed, with the perfume (or spice) prepared
It is spare after any of several broadleaf plants mud and apple butter mixing；
The man-made feeds prepared are layered in the pallet of 40cm wide, 65cm long, control thickness is not higher than 1cm, is cooled to
It after semisolid, is placed in cage of corresponding size, the Drosophila melanogaster adult 50 for discharging egg production peak time is right, controls cage net
The mesh number of cover is 60 mesh, prevents drosophila from escaping；It after oviposition for 24 hours, takes out pallet and is placed in insectary, after 3-4 days, to its hair
Educate for mature larva, through detecting, quantity is about 1500-2000 head, and then obtain containing the Drosophila melanogaster mature larva and
The Agar substrate of parasitic feed；
Choose diameter be 8cm agar ball (formed agar ball concentration be 2.5%), using spherical die by it is above-mentioned containing
The Agar substrate of the Drosophila melanogaster mature larva and parasitic feed is coated on the outside of the agar ball, controls the medium packet
Coating with a thickness of 2cm or so, the diameter for obtaining the parasitic agent is 10cm or so；By parasitic agent one obtained above
It is divided into four, includes Drosophila melanogaster mature larva about 400-500 head in every part, be wrapped in around the outer layer of agar ball respectively；So
The parasitic agent is fitted into scalable mesh bag afterwards, hangs on the raising container of 4 identical structure snd size after tension respectively
On the suspension iron wire of top, and a filter paper, as 4 parallel repeating samples are respectively placed in the bottom of described device；
(3) in each breeding apparatus, 10 pairs of parasitic wasps (quantitative proportion for controlling female drone is 1:1) is placed, is placed in
In the insectary of the same terms, after 3-4 days, the breeding apparatus that the parasitic wasp of survival is more renewed, until parasitic wasp oviposition terminates
It is dead afterwards；
(4) after 15-20 days, the parasitic wasp to sprout wings out in each breeding apparatus is transferred in new raising container expand it is numerous.
Embodiment 2
The method of interior raising drosophila parasitic wasp described in the present embodiment, the breeding apparatus based on structure shown in Fig. 1-2 into
Row, specifically comprises the following steps:
(1) field acquisition Drosophila melanogaster larva and pupa, are placed in cage in the insectary of suitable condition, to its emergence
It waits expanding as worm kind after parasitic wasp out numerous；Control the condition of the artificial feeding are as follows: 26 ± 1 DEG C of raising temperature, relative humidity 50-
60%, light application time 16L:8D；
(2) man-made feeds are prepared: weighing banana 100g, apple 130g, pureed is made with juice extractor after being mixed；Separately
Banana corn flour 40g, sucrose 25g, yeast powder 20g, preservative (potassium sorbate) 2g, agar 8g are weighed, with the banana prepared
It is spare after mud and apple butter mixing.
The man-made feeds prepared are layered in the pallet of 40cm wide, 65cm long, control thickness is not higher than 1cm, is cooled to
It after semisolid, is placed in cage of corresponding size, the Drosophila melanogaster adult 30 for discharging egg production peak time is right, cage mesh enclosure
Mesh number is 60 mesh, prevents drosophila from escaping；After oviposition for 24 hours, takes out pallet and be placed in the insectary of the same terms, after 3-4 days,
It is mature larva to its development, through detecting, quantity is about 900-1200 head, and then is obtained containing the aging children of the Drosophila melanogaster
The Agar substrate of worm and parasitic feed；
Choose diameter be 8cm agar ball (formed agar ball concentration be 2.5%), using spherical die by it is above-mentioned containing
The Agar substrate of the Drosophila melanogaster mature larva and parasitic feed is coated on the outside of the agar ball, controls the medium packet
Coating with a thickness of 2cm or so, the diameter for obtaining the parasitic agent is 10cm or so；By parasitic agent one obtained above
It is divided into four, every part includes Drosophila melanogaster mature larva 250-300 head, is wrapped in around agar ball respectively；Then it is posted described
Raw medium is fitted into scalable mesh bag, hangs on the suspension above the raising container of 4 identical structure snd size after tension respectively
On iron wire, and a filter paper, as 4 parallel repeating samples are respectively placed in the bottom of described device；
(3) in each breeding apparatus, 5 pairs of parasitic wasps (quantitative proportion for controlling female drone is 1.5:1) is placed, is placed in
In the insectary of the same terms, after 3-4 days, the breeding apparatus that the parasitic wasp of survival is more renewed, meeting after parasitic wasp lays eggs
It is dead；
(6) after 15-20 days, the parasitic wasp out that sprouts wings is transferred in new raising container expand it is numerous.
Embodiment 3
The method of interior raising drosophila parasitic wasp described in the present embodiment, the breeding apparatus based on structure shown in Fig. 1-2 into
Row, specifically comprises the following steps:
(1) field acquisition Drosophila melanogaster larva and pupa, are placed in cage in the insectary of suitable condition, to its emergence
It waits expanding as worm kind after parasitic wasp out numerous；Control the condition of the artificial feeding are as follows: 26 ± 1 DEG C of raising temperature, relative humidity 50-
60%, light application time 16L:8D；
(2) man-made feeds are prepared: weighing banana 130g, apple 100g, pureed is made with juice extractor after being mixed；Separately
Banana corn flour 50g, sucrose 20g, yeast powder 25g, preservative (potassium sorbate) 1g, agar 10g are weighed, with the banana prepared
It is spare after mud and apple butter mixing；
The man-made feeds prepared are layered in the pallet of 30-50cm wide, 50-80cm long, thickness is not higher than 1cm, cooling
It after semisolid, is placed in cage of corresponding size, the Drosophila melanogaster adult 50 for discharging egg production peak time is right, cage mesh enclosure
Mesh number be 60 mesh, prevent drosophila from escaping；After oviposition for 24 hours, takes out pallet and be placed in the insectary of the same terms, after 3-4 days,
It is mature larva to its development, through detecting, quantity is about 1500-2000 head, and then is obtained aging containing the Drosophila melanogaster
The Agar substrate of larva and parasitic feed；
Choose diameter be 6cm agar ball (formed agar ball concentration be 2.5%), using spherical die by it is above-mentioned containing
The Agar substrate of the Drosophila melanogaster mature larva and parasitic feed is coated on the outside of the agar ball, controls the medium packet
Coating with a thickness of 2cm or so, the diameter for obtaining the parasitic agent is 8cm or so；By parasitic agent one obtained above
It is divided into five, every part includes Drosophila melanogaster mature larva 300-400 head, is wrapped in around agar ball respectively；Then it is posted described
Raw medium is fitted into scalable mesh bag, hangs on the suspension above the raising container of 4 identical structure snd size after tension respectively
On iron wire, and a filter paper, as 5 parallel repeating samples are respectively placed in the bottom of described device；
(3) in each breeding apparatus, 8 pairs of parasitic wasps (quantitative proportion for controlling female drone is 3:1) is placed, phase is placed in
With in the insectary of condition, after 3-4 days, the breeding apparatus that the parasitic wasp of survival is more renewed, parasitic wasp can be dead after laying eggs
It dies；
(4) after 15-20 days, the parasitic wasp out that sprouts wings is transferred in new raising container expand it is numerous.
Comparative example 1
The method of interior raising drosophila parasitic wasp, same as Example 1 described in this comparative example, and difference is only that described post
Raw medium is not provided in the mesh bag, and is placed in the bottom position of the raising container, is specifically comprised the following steps:
(1) field acquisition Drosophila melanogaster larva and pupa, are placed in cage in the insectary of suitable condition, to its emergence
It waits expanding as worm kind after parasitic wasp out numerous；Control the condition of the artificial feeding are as follows: 26 ± 1 DEG C of raising temperature, relative humidity 50-
60%, light application time 16L:8D；
(2) man-made feeds are prepared: weighing banana 120g, apple 120g, pureed is made with juice extractor after being mixed；Separately
Banana corn flour 45g, sucrose 22g, yeast powder 22g, preservative (potassium sorbate) 1.5g, agar 9g are weighed, with the perfume (or spice) prepared
It is spare after any of several broadleaf plants mud and apple butter mixing；
The man-made feeds prepared are layered in the pallet of 40cm wide, 65cm long, control thickness is not higher than 1cm, is cooled to
It after semisolid, is placed in cage of corresponding size, the Drosophila melanogaster adult 50 for discharging egg production peak time is right, controls cage net
The mesh number of cover is 60 mesh, prevents drosophila from escaping；It after oviposition for 24 hours, takes out pallet and is placed in insectary, after 3-4 days, to its hair
Educate for mature larva, through detecting, quantity is about 1500-2000 head, and then obtain containing the Drosophila melanogaster mature larva and
The Agar substrate of parasitic feed；
Choose diameter be 8cm agar ball (formed agar ball concentration be 2.5%), using spherical die by it is above-mentioned containing
The Agar substrate of the Drosophila melanogaster mature larva and parasitic feed is coated on the outside of the agar ball, controls the medium packet
Coating with a thickness of 2cm or so, the diameter for obtaining the parasitic agent is 10cm or so；By parasitic agent one obtained above
It is divided into four, includes Drosophila melanogaster mature larva about 400-500 head in every part, be wrapped in around the outer layer of agar ball respectively；So
The parasitic agent is laid in afterwards at the bottom of bottle position of the parasitic container；
(3) in each raising bottle, 10 pairs of parasitic wasps (quantitative proportion for controlling female drone is 1:1) is placed, phase is placed in
With in the insectary of condition, after 3-4 days, the breeding apparatus that the parasitic wasp of survival is more renewed, parasitic wasp can be dead after laying eggs
It dies；
(4) after 15-20 days, the parasitic wasp out that sprouts wings is transferred in new raising container expand it is numerous.
Comparative example 2
The method of interior raising drosophila parasitic wasp described in this comparative example, the breeding apparatus based on structure shown in Fig. 1-2 into
Row, specifically comprises the following steps:
(1) field acquisition Drosophila melanogaster larva and pupa, are placed in cage in the insectary of suitable condition, to its emergence
It waits expanding as worm kind after parasitic wasp out numerous；Control the condition of the artificial feeding are as follows: 26 ± 1 DEG C of raising temperature, relative humidity 50-
60%, light application time 16L:8D；
(2) man-made feeds are prepared: weighing banana 120g, apple 120g, pureed is made with juice extractor after being mixed；Separately
Banana corn flour 45g, sucrose 22g, yeast powder 22g, preservative (potassium sorbate) 1.5g, agar 9g are weighed, with the perfume (or spice) prepared
It is spare after any of several broadleaf plants mud and apple butter mixing；
The man-made feeds prepared are layered in the pallet of 40cm wide, 65cm long, control thickness is not higher than 1cm, is cooled to
It after semisolid, is placed in cage of corresponding size, the Drosophila melanogaster adult 50 for discharging egg production peak time is right, controls cage net
The mesh number of cover is 60 mesh, prevents drosophila from escaping；It after oviposition for 24 hours, takes out pallet and is placed in insectary, after 3-4 days, to its hair
It educates for mature larva, through detecting, quantity is about 1600-2000 head；, and then obtain containing the Drosophila melanogaster mature larva and
The Agar substrate of parasitic feed；
Choose diameter be 8cm agar ball (formed agar ball concentration be 2.5%), using spherical die by it is above-mentioned containing
The Agar substrate of the Drosophila melanogaster mature larva and parasitic feed is coated on the outside of the agar ball, controls the medium packet
Coating with a thickness of 2cm or so, the diameter for obtaining the parasitic agent is 10cm or so；By parasitic agent one obtained above
It is divided into four, includes Drosophila melanogaster mature larva about 400-500 head in every part, be wrapped in around the outer layer of agar ball respectively；So
The parasitic agent is fitted into scalable mesh bag afterwards, hangs on the raising container of 4 identical structure snd size after tension respectively
On the suspension iron wire of top, and a filter paper, as 4 parallel repeating samples are respectively placed in the bottom of described device；
(3) in each breeding apparatus, 5 pairs of parasitic wasps (quantitative proportion for controlling female drone is 1.5:1) is placed, is placed in
In the insectary of the same terms, after 3-4 days, the breeding apparatus that the parasitic wasp of survival is more renewed, meeting after parasitic wasp lays eggs
It is dead；
(4) after 15-20 days, the parasitic wasp out that sprouts wings is transferred in new raising container expand it is numerous.
Comparative example 3
The method of interior raising drosophila parasitic wasp described in the present embodiment, the breeding apparatus based on structure shown in Fig. 1-2 into
Row, specifically comprises the following steps:
(1) field acquisition Drosophila melanogaster larva and pupa, are placed in cage in the insectary of suitable condition, to its emergence
It waits expanding as worm kind after parasitic wasp out numerous；Control the condition of the artificial feeding are as follows: 26 ± 1 DEG C of raising temperature, relative humidity 50-
60%, light application time 16L:8D；
(2) man-made feeds are prepared: weighing banana 120g, apple 120g, pureed is made with juice extractor after being mixed；Separately
Banana corn flour 45g, sucrose 22g, yeast powder 22g, preservative (potassium sorbate) 1.5g, agar 9g are weighed, with the perfume (or spice) prepared
It is spare after any of several broadleaf plants mud and apple butter mixing；
(man-made feeds prepared are layered in the pallet of 40cm wide, 65cm long, control thickness is not higher than 1cm, is cooled to
It after semisolid, is placed in cage of corresponding size, the Drosophila melanogaster adult 50 for discharging egg production peak time is right, controls cage net
The mesh number of cover is 60 mesh, prevents drosophila from escaping；It after oviposition for 24 hours, takes out pallet and is placed in insectary, after 3-4 days, to its hair
Educate for mature larva, through detecting, quantity is about 1500-2000 head, and then obtain containing the Drosophila melanogaster mature larva and
The Agar substrate of parasitic feed；
Choose diameter be 8cm agar ball (formed agar ball concentration be 2.5%), using spherical die by it is above-mentioned containing
The Agar substrate of the Drosophila melanogaster mature larva and parasitic feed is coated on the outside of the agar ball, controls the medium packet
Coating with a thickness of 2cm or so, the diameter for obtaining the parasitic agent is 10cm or so；By parasitic agent one obtained above
It is divided into four, includes Drosophila melanogaster mature larva about 400-500 head in every part, be wrapped in around the outer layer of agar ball respectively；So
The parasitic agent is fitted into scalable mesh bag afterwards, hangs on the raising container of 4 identical structure snd size after tension respectively
On the suspension iron wire of top, and a filter paper, as 4 parallel repeating samples are respectively placed in the bottom of described device；
(3) in each breeding apparatus, 20 pairs of parasitic wasps (quantitative proportion for controlling female drone is 3:1) is placed, is placed in
In the insectary of the same terms, after 3-4 days, the breeding apparatus that the parasitic wasp of survival is more renewed, meeting after parasitic wasp lays eggs
It is dead；
(4) after 15-20 days, the parasitic wasp out that sprouts wings is transferred in new raising container expand it is numerous.
Above-described embodiment 1-3 and the quantity that compared the parasitic wasp after raising in 1-3 are detected, and calculates and removes table
Middle items life parameters, calculated result see the table below shown in 1.Wherein,
Drosophila eclosion rate (%) refers to and is not sprouted wings by parasitism that drosophila quantity out accounts for the ratio of all drosophila quantity；
Parasitic wasp bee hatching rate (%) refers to by parasitism and parasitic wasp quantity out of sprouting wings accounts for the ratio of all drosophila quantity；
Breeding amount (head/female) refers to algebra after parasitic wasp caused by every female parasitic wasp.
The life parameters of drosophila and parasitic wasp under the different rearing conditions of table 1
From upper table data it is found that in embodiment 1 and embodiment 2, drosophila is launched in control and the ratio of parasitic wasp is set separately
For 40-50:1 and 50-60:1, drosophila eclosion rate, parasitic wasp bee hatching rate and breeding amount difference are not obvious；And in embodiment 3,
The ratio that drosophila and parasitic wasp are launched in control is set as 40-50:1, but from embodiment 1 and 2 and unlike, simulate the ball of fruit
Shape media diameters are not identical, and diameter is respectively that 8cm and 6cm also adjust the quantity of host according to the size of its diameter
Whole, as can be seen from the results, drosophila eclosion rate, parasitic wasp bee hatching rate and breeding amount difference are not obvious.As long as illustrating drosophila and parasitism
The ratio of bee and the size control of agar ball have no effect on the feeding effect of parasitic wasp in a certain range.
And by by comparative example 1 compared with Example 1 compared with not obviously increased by parasitic drosophila quantity, and parasitic wasp
Bee hatching rate and breeding amount are declined, and for this explanation compared with traditional method for breeding, the shape of design parasitic medium is spherical shape
And suspension processing is carried out, the bee hatching rate and breeding amount of parasitic wasp are improved, helps to save cost, is reduced artificial.
By the way that comparative example 2 compares with embodiment 1, although the breeding amount of its parasitic wasp increases, under bee hatching rate is significant
Drop, drosophila eclosion rate significantly increase.This explanation, excessive host will cause its waste, also can after not sprouted wings by parasitic drosophila
The transfer of parasitic wasp is influenced, cost of labor is increased.Comparative example 3 is compared with embodiment 1, although parasitic wasp bee hatching rate increases,
But breeding amount is decreased obviously, this explanation, excessive parasitic wasp will also result in its waste, reduces overall reproductive amount.It can be seen that posting
The main ratio with parasitic wasp answers requirement according to the invention, and control is in a certain range.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (10)
1. a kind of device for indoor raising drosophila parasitic wasp, which is characterized in that including the raising appearance for parasitic wasp breeding
Device (1) is vacantly provided with the parasitic agent (3) for being suitable for parasitic wasp breeding in raising container (1)；The parasitic agent
It (3) is the Agar substrate for being mixed with the ball shape structure of drosophila host and host's feed.
2. the device according to claim 1 for indoor raising drosophila parasitic wasp, which is characterized in that the parasitic agent
It (3) include agar ball (5) and the media packs for being mixed with drosophila host and host's feed for being coated on agar ball (5) outer layer
Layer (4).
3. the device according to claim 1 or 2 for indoor raising drosophila parasitic wasp, which is characterized in that the parasitism
Medium (3) is contained in scalability mesh bag (2), and is hung in the raising container (1).
4. the device according to claim 1-3 for indoor raising drosophila parasitic wasp, which is characterized in that described
Drosophila host is the mature larva of Drosophila melanogaster.
5. the device according to claim 1-4 for indoor raising drosophila parasitic wasp, which is characterized in that described
Host's feed includes the component of following parts by weight: banana 100-130 parts by weight, apple 100-130 parts by weight, banana corn flour
40-50 parts by weight, sucrose 20-25 parts by weight, yeast powder 20-25 parts by weight, preservative 1-2 parts by weight and agar 8-10 weight
Measure part.
6. a kind of method of interior raising drosophila parasitic wasp, which is characterized in that any including parasitic wasp is placed in claim 1-5
The step of expanding propagation is carried out in device described in.
7. the method for raising drosophila parasitic wasp in interior according to claim 6, which comprises the steps of:
(1) field acquisition Drosophila melanogaster larva and pupa, and artificial feeding sprout wings parasitic wasp out to it after wait for as parasitic wasp worm kind
Expand it is numerous, it is spare；
(2) host's feed is made according to selected components and ratio, and carries out the drosophila host using host's feed
Raising, and the host's feed for being mixed with the drosophila host is coated on agar ectosphere, the parasitic agent is made simultaneously
It hangs in the raising container；
(3) parasitic wasp obtained in step (1) is placed in the raising container and expand numerous raising, after parasitic wasp lays eggs
It can be dead；
(4) parasitic wasp was raised to after 15-20 days, the parasitic wasp out that sprouts wings is transferred in new raising container and is expanded
It is numerous.
8. the method for raising drosophila parasitic wasp in interior according to claim 7, which is characterized in that in the step (1), institute
State the condition of artificial feeding are as follows: 26 ± 1 DEG C of raising temperature of control, relative humidity 50-60%, light application time 16L:8D.
9. the method for raising drosophila parasitic wasp in interior according to claim 7 or 8, which is characterized in that the step (2)
In, the preparation of the parasitic agent specifically includes:
Weigh a selected amount of banana and apple, with being made pureed after mixing, and with a selected amount of banana corn flour, sugarcane
Sugar, yeast powder, preservative and agar mix, and are placed in pallet and are cooled to semisolid；
Obtained parasitic feed semisolid is placed in cage, and the Drosophila melanogaster adult for discharging egg production peak time is bred；
After Drosophila melanogaster oviposition for 24 hours, it is aging to its development that taking-up pallet, which is placed in the insectary of the same terms, which breeds 3-4 days,
Larva is to get to the parasitic forage mixture for being mixed with required drosophila host；
The mixture of the resulting parasitic feed for being mixed with drosophila host is coated on agar ball surface, the required of ball shape structure is made
Parasitic agent.
10. according to the method for the described in any item indoor raising drosophila parasitic wasps of claim 7-9, which is characterized in that the step
Suddenly in (3), the supply volume ratio of the Drosophila melanogaster and the parasitic wasp is 40-60:1-1.5, and controls the described of dispensing and post
The male and female quantitative proportion of raw bee is 1-3:1.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109769755A (en) * | 2019-03-29 | 2019-05-21 | 福建农林大学 | A kind of simple and fast propagation method of spotted wing drosophila pupa parasitic wasp hair angle clavicorn serphid |
| CN110150232A (en) * | 2019-06-06 | 2019-08-23 | 贵州省烟草公司贵阳市公司 | A kind of method that batch raising microbracon hebetor bee pupa kills storage cacac moth |
| CN110742023A (en) * | 2019-10-25 | 2020-02-04 | 山东省葡萄研究院 | Breeding device and breeding method for fruit fly pupa parasitic wasps |
| CN110810337A (en) * | 2019-11-04 | 2020-02-21 | 山东省葡萄研究院 | Drosophila pupae parasitic wasp feeding bottle convenient to operate and operation method |
| CN111296368A (en) * | 2020-04-23 | 2020-06-19 | 福建农林大学 | Method for producing fruit fly parasitic wasps by using drosophila melanogaster |
| CN111543392A (en) * | 2020-04-27 | 2020-08-18 | 浙江大学 | Method and device for large-scale raising of fruit fly parasitic wasps |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918887A (en) * | 2014-04-22 | 2014-07-16 | 山东省农业科学院植物保护研究所 | Artificial feed for suzukii fruit bat fly |
| CN103931565A (en) * | 2014-04-22 | 2014-07-23 | 山东省农业科学院植物保护研究所 | Artificial breeding method of Suzuki fruit flies for testing |
| CN203735281U (en) * | 2014-02-20 | 2014-07-30 | 宁波市林特科技推广中心 | Simple pachycrepoideus vindemmiae releasing device |
| CN204860629U (en) * | 2015-07-07 | 2015-12-16 | 中国农业科学院草原研究所 | Parasitic device is raised to parasitics insect |
| CN105794728A (en) * | 2016-04-07 | 2016-07-27 | 云南省农业科学院农业环境资源研究所 | Indoor breeding method for drosophila-suzukii-matsumura advantageous parasitic wasps trichopria drosophilae |
-
2018
- 2018-11-05 CN CN201811308993.3A patent/CN109496990B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203735281U (en) * | 2014-02-20 | 2014-07-30 | 宁波市林特科技推广中心 | Simple pachycrepoideus vindemmiae releasing device |
| CN103918887A (en) * | 2014-04-22 | 2014-07-16 | 山东省农业科学院植物保护研究所 | Artificial feed for suzukii fruit bat fly |
| CN103931565A (en) * | 2014-04-22 | 2014-07-23 | 山东省农业科学院植物保护研究所 | Artificial breeding method of Suzuki fruit flies for testing |
| CN204860629U (en) * | 2015-07-07 | 2015-12-16 | 中国农业科学院草原研究所 | Parasitic device is raised to parasitics insect |
| CN105794728A (en) * | 2016-04-07 | 2016-07-27 | 云南省农业科学院农业环境资源研究所 | Indoor breeding method for drosophila-suzukii-matsumura advantageous parasitic wasps trichopria drosophilae |
Non-Patent Citations (1)
| Title |
|---|
| 耿军灵等: "不同琼脂配比对阿里山潜蝇茧蜂寄生效果的影响", 《福建林学院学报》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109769755A (en) * | 2019-03-29 | 2019-05-21 | 福建农林大学 | A kind of simple and fast propagation method of spotted wing drosophila pupa parasitic wasp hair angle clavicorn serphid |
| CN109769755B (en) * | 2019-03-29 | 2021-09-07 | 福建农林大学 | Simple and rapid propagation method of euproctis maculata pupa parasitic wasps, namely horned and branchlet wasps |
| CN110150232A (en) * | 2019-06-06 | 2019-08-23 | 贵州省烟草公司贵阳市公司 | A kind of method that batch raising microbracon hebetor bee pupa kills storage cacac moth |
| CN110150232B (en) * | 2019-06-06 | 2021-11-02 | 贵州省烟草公司贵阳市公司 | Method for killing stored tobacco meal moth by batch breeding of meadow moth braconid bees and pupas |
| CN110742023A (en) * | 2019-10-25 | 2020-02-04 | 山东省葡萄研究院 | Breeding device and breeding method for fruit fly pupa parasitic wasps |
| CN110810337A (en) * | 2019-11-04 | 2020-02-21 | 山东省葡萄研究院 | Drosophila pupae parasitic wasp feeding bottle convenient to operate and operation method |
| CN110810337B (en) * | 2019-11-04 | 2022-03-11 | 山东省葡萄研究院 | Drosophila pupae parasitic wasp feeding bottle convenient to operate and operation method |
| CN111296368A (en) * | 2020-04-23 | 2020-06-19 | 福建农林大学 | Method for producing fruit fly parasitic wasps by using drosophila melanogaster |
| CN111543392A (en) * | 2020-04-27 | 2020-08-18 | 浙江大学 | Method and device for large-scale raising of fruit fly parasitic wasps |
| CN111543392B (en) * | 2020-04-27 | 2022-01-18 | 浙江大学 | Method and device for large-scale raising of fruit fly parasitic wasps |
| CN113892465A (en) * | 2021-10-08 | 2022-01-07 | 福建艳璇生物防治技术有限公司 | Method for automatically propagating fruit fly parasitic wasps in field |
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