CN110810337A - Drosophila pupae parasitic wasp feeding bottle convenient to operate and operation method - Google Patents

Abstract

The easy-to-operate breeding bottle of the Drosophila pupal parasitoid is used to facilitate the breeding of the Drosophila pupal parasitoid. It includes: a single bottle, three of which are arranged side by side; a conduit, which is provided with conduits at the left and right ends of each single bottle, and two adjacent single bottles are connected together by conduits; an opening and closing unit, The opening and closing unit is arranged on the duct to realize the opening and closing of the duct; the bee-blocking unit is arranged on the bottle mouth of the single bottle to prevent parasitic wasps from flying out of the single bottle, but does not affect the air exchange inside and outside the single bottle . The easy-to-operate feeding bottle and the operation method of the Drosophila pupa parasitoid provided by the invention can realize the breeding of the parasitic wasps; The transfer between them is convenient and simple to operate.

Description

Raising bottle of Drosophila pupal parasitoid that is easy to operate and operation method

technical field

The invention relates to the technical field of breeding of Drosophila pupa parasitoids, in particular to a breeding bottle for Drosophila pupa parasitoids and an operation method that is easy to operate.

Background technique

As a pest, Drosophila seriously threatens the yield and quality of cherries and peaches. In the prevention and control of fruit flies, biological control is the control measure with the least pollution to the environment. The mass reproduction of Drosophila biological control is achieved by placing Drosophila pupal parasitoids. Before placing, the Drosophila pupa parasitoids need to be bred. During the breeding process, when the Drosophila pupae are parasitized, the parasitoids need to be taken out and transferred to other breeding bottles for continued breeding. This requires regular transfer of parasitic wasps, which undoubtedly increases the workload of breeders.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a kind of feeding bottle and operation method of the Drosophila pupa parasitoid that is easy to operate, and is used to facilitate the breeding of the Drosophila pupa parasitoid.

The technical scheme adopted by the present invention to solve the technical problem is: the feeding bottle of the Drosophila pupa parasitoid is easy to operate, and it is characterized in that it comprises:

A single bottle, the single bottle is three arranged side by side;

A conduit, the left and right ends of each single bottle are provided with conduits, and two adjacent single bottles are connected together by conduits;

an opening and closing unit, the opening and closing unit is arranged on the conduit to realize opening and closing of the conduit;

The bee blocking unit, which is arranged on the bottle mouth of the single bottle, prevents parasitic wasps from flying out of the single bottle, but does not affect the air exchange inside and outside the single bottle.

Further, the opening and closing unit includes a sliding rod, a baffle plate fixed at the end of the sliding rod, and a sealing ring arranged in the conduit, the sliding rods are three evenly arranged along the same circumference, and the sliding rods are slidably installed on the side wall of the conduit. ; The sealing ring is a circular structure, the inner wall of the sealing ring is provided with a plate groove, the baffle is a fan-shaped structure and is slidably connected with the plate groove, when the three baffles are in contact with each other, the conduit is closed; the baffle moves to The conduit is opened when the sealing ring is in the groove of the plate.

Further, a through hole matched with the sliding rod is provided on the side wall of the conduit, and a damping member is provided between the through hole and the sliding rod.

Further, the opening and closing unit includes a rotating shaft rotatably installed on the side wall of the guide tube, a handle fixed on the upper end of the rotating shaft, and a ball valve fixed on the lower end of the rotating shaft and placed inside the guide tube. There is a stopper, the outer wall of the stopper is in contact with the inner wall of the conduit, the first end of the stopper is provided with a spherical surface contacting the outer wall of the ball valve, the second end of the stopper is provided with a communication hole, and the conduit communicates with the circular hole through the communication hole.

Further, a sealing strip is arranged on the first contact surface of the baffle, and a long groove is arranged on the second contact surface of the baffle; between two adjacent baffles, the seal on the first baffle is The strip extends into the long groove of the second baffle, and the sealing strip on the second baffle extends into the long groove of the first baffle.

Further, a support body is fixed on the top of the conduit, and the rotating shaft is rotatably connected with the support body.

Further, the bee blocking unit comprises a bottle cap threadedly connected with the bottle mouth, a hanging plate arranged on the inner side of the bottle cap, a radial rod arranged between the hanging plate and the inner wall of the bottle cap, and a radial rod arranged between adjacent radial bars. And the gauze between the inner walls of the bottle cap, and the sealing plate is threaded on the top of the bottle cap.

Further, a limit ring fixedly connected with the inner wall of the bottle cap is arranged above the radial rod, and the limit ring is used for contacting with the sealing plate.

Further, a hanging hole is provided in the center of the hanging plate, and the hanging hole is used for hanging the breeding bag.

The operation method of the feeding bottle of the Drosophila pupa parasitoid that is easy to operate, is characterized in that, may further comprise the steps:

(1) hanging the breeding bag on the bottle cap of the first single bottle, and making the breeding bag placed in the first single bottle;

(2) When most of the third instar larvae in the breeding bag in the first single bottle develop fruit fly pupae and adhere to the inner wall of the first single bottle, put the parasitic wasp into the second single bottle, and seal the first single bottle. The conduit at the right end of the bottle, open the conduits at the left end of the first single bottle, both ends of the second single bottle and both ends of the third single bottle, close the caps of the second single bottle and the third single bottle through the sealing plate, and then Blow air into the tube at the left end of the third single bottle, and the parasitic wasps in the second single bottle are transferred to the first single bottle; then, close the tube at the left end of the first single bottle;

(3) Hang the breeding bag on the cap of the second single bottle, place the breeding bag in the second single bottle, remove the sealing plate on the cap of the second single bottle, and close the second single bottle conduits at both ends;

(4) After the fruit fly pupae in the first single bottle are exhausted, install a sealing plate on the bottle cap of the first single bottle, remove the sealing plate on the bottle cap of the second single bottle, and open the second single bottle. Then, blow air into the tube at the right end of the first single bottle, transfer the parasitic wasps in the first single bottle to the second single bottle, and then close the tube at the right end of the second single bottle. , the parasitic wasps continue to breed in the second single bottle;

(5) Hang the breeding bag on the bottle cap of the third single bottle, place the breeding bag in the third single bottle, remove the sealing plate on the bottle cap of the third single bottle, and close the third single bottle conduits at both ends;

(6) After the fruit fly pupae in the second single bottle are exhausted, install a sealing plate on the bottle cap of the second single bottle, remove the sealing plate on the bottle cap of the third single bottle, and open the third single bottle. Then blow air into the tube at the right end of the first single bottle, transfer the parasitic wasps in the second single bottle to the third single bottle, and then close the tube at the right end of the third single bottle , at which time the parasitoid continued to breed in the third single bottle until maturity.

The beneficial effects of the invention are as follows: the easy-to-operate breeding bottle and the operation method of the Drosophila pupa parasitoid can realize the breeding of the parasitoid; The transfer of parasitic wasps between individual bottles is convenient and simple to operate.

Description of drawings

Fig. 1 is the front view of the feeding bottle of Drosophila pupal parasitoid;

Fig. 2 is a three-dimensional view of the rearing bottle after removing the gauze;

Fig. 3 is the top view of Fig. 2;

Fig. 4 is the right side view of Fig. 2;

Figure 5 is a sectional view of a single bottle;

Figure 6 is a side view of the catheter;

7 is a schematic diagram of the opening and closing unit of the first embodiment;

Fig. 8 is A-A sectional view in Fig. 3;

9 is a schematic diagram of the installation of the opening and closing unit of the second embodiment;

10 is a schematic diagram of the opening and closing unit of the second embodiment;

Figure 11 is a bottom view of the bottle cap;

Figure 12 is a top view of the bottle cap;

Figure 13 is a sectional view of the bottle cap;

Figure 14 is a schematic diagram of hanging the breeding bag on the bottle cap

Figure 15 is a schematic diagram when transferring parasitic wasps;

Figure 16 is a schematic view of a sealing ring;

Figure 17 is a schematic diagram of the assembly of the ball valve in the conduit;

In the picture: 1 single bottle, 11 bottle mouth, 12 placement surface, 13 conduit mounting surface, 2 conduits, 20 through holes, 21 sliding rods, 22 sealing rings, 23 baffles, 24 sealing strips, 25 ball valves, 26 round holes, 27 shaft, 28 handle, 29 support, 3 gauze, 31 rubber band, 4 bottle cap, 41 hanging plate, 42 lifting hole, 43 radial rod, 44 gauze, 45 limit ring, 46 sealing plate, 47 handle, 5 Breeding bag, 51 knots, 6 blocks, 61 spherical surfaces, 62 connecting holes.

Detailed ways

As shown in FIG. 1 to FIG. 17 , the feeding bottle of the present invention mainly includes a single bottle 1 , a conduit 2 and an opening and closing unit. The present invention will be described in detail below with reference to the accompanying drawings.

As shown in Figures 1 to 5, the single bottle 1 is a transparent glass bottle or plastic bottle, and the bottom surface of the single bottle is a horizontal plane. When in use, the bottom surface is contacted with the desktop to make the single bottle in an upright state; the outer wall of the single bottle is provided with There is a pair of duct installation surfaces 13 arranged in parallel, the duct installation surface is a vertical surface, and a placement surface 12 is also provided on the outer wall of the single bottle. .

A conduit 2 is fixed on the conduit installation surface, the conduit is a transparent round tube, and the conduit and the single bottle are integrally formed. An opening and closing unit is provided on the duct. As shown in FIG. 5 , three through holes 20 are arranged on the side wall of the duct evenly arranged along the same circumference. As shown in FIG. 6 , a sliding rod 21 is slidably installed in the through holes. , the first end of the sliding rod extends into the duct, the second end of the sliding rod protrudes out of the duct, the first end of the sliding rod is fixed with a baffle 23, the baffle is a fan-shaped structure, and a sealing ring 22 is fixed on the inner wall of the duct , the sealing ring is a circular structure, as shown in Figure 16, and the inner wall of the sealing ring is provided with three evenly arranged plate grooves 221, and the baffles are slidably arranged in the corresponding plate grooves. Pull the slide bar outward until the baffle plate contacts the inner wall of the conduit, and the baffle plate moves completely into the plate groove; push the slider bar inward until the three baffle plates are in contact with each other, the baffle plate closes the inner cavity of the conduit. As shown in Figure 7, in order to ensure the sealing effect of the baffle on the inner cavity of the catheter, a long groove is provided on one of the contact surfaces of the baffle, and a sealing strip 24 is provided on the other contact surface of the baffle. When the sealing strip on the baffle extends into the long groove of the adjacent baffle, and the sealing strip on the adjacent baffle extends into the long groove of the certain baffle, the tightness between the contact surfaces of the three baffles better. A damping member is arranged between the sliding rod and the through hole to avoid any sliding between the sliding rod and the conduit. The sliding rod, the sealing ring, the baffle and the sealing strip constitute the opening and closing unit, and the conduction and closing of the conduit are realized through the opening and closing unit, and the position of the entire opening and closing unit on the conduit makes the opening and closing unit close to the single bottle. A support body 29 is provided on the top of the conduit, the rotating shaft is rotatably mounted on the support body, and the support body can be a bearing.

As shown in FIG. 2 , the rearing bottle of the present invention includes at least three single bottles, and the single bottles are arranged on the same straight line, and two adjacent single bottles are connected together by conduits. The dimensions of the conduits on two adjacent single bottles are different; however, in two adjacent single bottles, as shown in Figure 8, the conduits on one of the single bottles can extend into the inside of the conduits of the other single bottle, and the two conduits are close contact between them. When three single bottles are provided, the outer diameter of the conduit on the single bottle in the middle is equal to the inner diameter of the conduit on the other two single bottles.

When in use, three single vials are connected together by conduits, and all conduits are closed by opening and closing units. In the first single bottle, hang the breeding bag 5 containing the third instar larvae of Drosophila and food, wrap the gauze 3 on the bottle mouth 11 of the single bottle, and fasten the gauze with the elastic band 31 . When the third instar larvae grow into Drosophila pupae and attach to the inner wall of the single bottle, parasitic wasps are put into the first single bottle, and the parasitic wasps will grow on the Drosophila pupae. At the same time, add the breeding bag to the second single bottle. When the fruit fly pupae in the first single bottle are exhausted, the parasitic wasps in the first single bottle are taken out by the bee suction device and transferred to the second single bottle. At this time, most of the fruit fly pupae in the second single bottle are The third instar larvae of the fly grow into Drosophila pupae and attach to the inner wall of the single bottle; after the parasitic wasps have been bred in the second single bottle for a period of time, a breeding bag is added to the third single bottle, when the second single bottle After the fruit fly pupae are used up, the parasitic wasps are taken out again and placed in the third single bottle. At this time, most of the third instar larvae in the third single bottle grow into Drosophila pupae attached to the inner wall of the single bottle. When the fruit fly pupae in the third single bottle are exhausted, the parasitic wasps have developed into adult bees and can be used for placement in the field. The gauze constitutes a bee-blocking unit that blocks the mouth of a single bottle and prevents parasitic wasps from flying out of the single bottle.

As shown in Figures 9 and 10, as another structural form of the opening and closing unit, the opening and closing unit includes a ball valve 25, a rotating shaft 27 fixed on the ball valve, and a handle 28 fixed on the rotating shaft. It is rotatably mounted on the side wall of the conduit, and the handle is arranged above the conduit. There is a round hole 26 on the ball valve, and by rotating the knob, the ball valve can be rotated in the conduit. When the axis of the round hole is collinear with the axis of the conduit, the conduit is completely opened; as shown in Figure 17, when the axis of the round hole is perpendicular to the axis of the conduit , most of the catheter lumen is closed. In order to realize the complete closure of the conduit, a stopper 6 is respectively provided on both sides of the ball valve along the axis of the conduit. The outer wall of the ball valve is in close contact, and when the axis of the circular hole is perpendicular to the axis of the conduit, the two stoppers cooperate to realize the closure of the circular hole, thereby realizing the complete closure of the conduit. When the axis of the circular hole is collinear with the axis of the catheter, the setting of the communication hole makes the circular hole communicate with the inner cavity of the catheter.

In order to transfer the parasitic wasps in the first single bottle to the second single bottle without opening the bottle mouth, screw the bottle cap 4 on the bottle mouth, as shown in Figure 11 to Figure 13, the bottle cap 4 It is a circular structure, a circular hanging plate 41 is arranged on the inner side of the bottle cap, and at least three radial rods 43 are arranged between the hanging plate and the inner wall of the bottle cap. connection between. As shown in FIG. 14 , a hanging hole 42 is provided in the center of the hanging plate. During installation, the hanging rope of the breeding bag 5 is stretched into the hanging hole, and a knot 51 is attached to the upper end of the hanging rope, and the size of the knot is larger than the hanging hole, thereby realizing the suspension of the breeding bag 5 . A gauze 44 is arranged between the adjacent radial rods and the inner wall of the bottle cap, which can prevent parasitic wasps from flying out of the single bottle under the premise of ensuring the air circulation in the single bottle. An annular limit ring 45 is arranged above the radial rod, and the outer wall of the limit ring is fixed in contact with the inner wall of the bottle cap. A circular sealing plate 46 threadedly connected to the inner wall of the bottle cap is arranged above the limiting ring, and a handle 47 is fixed on the sealing plate. The bottle cap, gauze, radial rod and hanging plate constitute a bee blocking unit that can block the bottle mouth of a single bottle and prevent parasitic wasps from flying out of the single bottle.

When breeding parasitic wasps in a single bottle, remove the sealing plate to ensure good air permeability. When it is necessary to transfer the parasitic wasps in the first single bottle to the second single bottle, as shown in Figure 15, open the two conduits on the first single bottle, and place the left end of the second single bottle. The conduit is closed and the conduit at the right end is opened. Install the sealing plate on the top of the bottle cap on the first single bottle, and then blow air into the first single bottle through the conduit at the right end of the first single bottle. The parasitic wasps in the bottle are blown into the second single bottle, until the parasitic wasps in the first single bottle are completely transferred to the second single bottle, the conduit at the right end of the second single bottle is closed, and finally the first single bottle is closed. The sealing plates on the bottle caps on the two single bottles are opened, and the parasitic wasps can continue to breed in the second single bottle at this time. In the same way, the parasitic wasps in the second single bottle can be transferred to the third single bottle by the above method.

The operation method of the rearing bottle of the Drosophila pupa parasitoid that is easy to operate, comprises the following steps:

(1) hanging the breeding bag on the bottle cap of the first single bottle, and making the breeding bag placed in the first single bottle;

(2) When most of the third instar larvae in the breeding bag in the first single bottle develop fruit fly pupae and adhere to the inner wall of the first single bottle, put the parasitic wasp into the second single bottle, and seal the first single bottle. The conduit at the right end of the bottle, open the conduits at the left end of the first single bottle, both ends of the second single bottle and both ends of the third single bottle, close the caps of the second single bottle and the third single bottle through the sealing plate, and then Blow air into the tube at the left end of the third single bottle, and the parasitic wasps in the second single bottle are transferred to the first single bottle; then, close the tube at the left end of the first single bottle;

(3) Hang the breeding bag on the cap of the second single bottle, place the breeding bag in the second single bottle, remove the sealing plate on the cap of the second single bottle, and close the second single bottle conduits at both ends;

(4) After the fruit fly pupae in the first single bottle are exhausted, install a sealing plate on the bottle cap of the first single bottle, remove the sealing plate on the bottle cap of the second single bottle, and open the second single bottle. Then, blow air into the tube at the right end of the first single bottle, transfer the parasitic wasps in the first single bottle to the second single bottle, and then close the tube at the right end of the second single bottle. , the parasitic wasps continue to breed in the second single bottle;

(5) Hang the breeding bag on the bottle cap of the third single bottle, place the breeding bag in the third single bottle, remove the sealing plate on the bottle cap of the third single bottle, and close the third single bottle conduits at both ends;

(6) After the fruit fly pupae in the second single bottle are exhausted, install a sealing plate on the bottle cap of the second single bottle, remove the sealing plate on the bottle cap of the third single bottle, and open the third single bottle. Then blow air into the tube at the right end of the first single bottle, transfer the parasitic wasps in the second single bottle to the third single bottle, and then close the tube at the right end of the third single bottle , at which time the parasitoid continued to breed in the third single bottle until maturity.

Claims (10)

1. the rearing bottle of the Drosophila pupa parasitoid that is easy to operate, is characterized in that, it comprises: A single bottle, the single bottle is three arranged side by side; A conduit, the left and right ends of each single bottle are provided with conduits, and two adjacent single bottles are connected together by conduits; an opening and closing unit, the opening and closing unit is arranged on the conduit to realize opening and closing of the conduit; The bee blocking unit, which is arranged on the bottle mouth of the single bottle, prevents parasitic wasps from flying out of the single bottle, but does not affect the air exchange inside and outside the single bottle. 2. the rearing bottle of the Drosophila pupa parasitoid that is easy to operate according to claim 1, it is characterized in that, the opening and closing unit comprises sliding rod, the baffle plate that is fixed at the end of the sliding rod, the sealing ring that is arranged in the conduit, so The sliding rods are three uniformly arranged along the same circumference, and the sliding rods are slidably installed on the side wall of the conduit; the sealing ring is a circular ring structure, the inner wall of the sealing ring is provided with a plate groove, and the baffle is a fan-shaped structure and is connected with the plate groove. Sliding connection, when the three baffles are in contact with each other, the conduit is closed; when the baffle moves into the plate groove on the sealing ring, the conduit is opened. 3. the rearing bottle of the Drosophila pupa parasitoid that is easy to operate according to claim 2, it is characterized in that, be provided with the through hole that cooperates with the sliding rod on the side wall of the conduit, be provided with between the through hole and the sliding rod Damping parts. 4. the rearing bottle of the Drosophila pupa parasitoid that is easy to operate according to claim 3, it is characterized in that, the opening and closing unit comprises the rotating shaft that is rotatably installed on the side wall of the duct, the handle that is fixed on the upper end of the rotating shaft, is fixed on the lower end of the rotating shaft The ball valve placed on the inner side of the duct is provided with a round hole on the ball valve, and blocks are respectively provided on both sides of the ball valve. A communication hole is provided at the second end of the stopper, and the conduit communicates with the circular hole through the communication hole. 5. the rearing bottle of the easy-to-operate Drosophila pupa parasitoid according to claim 1, is characterized in that, be provided with sealing strip on the first contact surface of baffle plate, on the second contact surface of baffle plate There is a long groove; between two adjacent baffles, the sealing strip on the first baffle extends into the long groove of the second baffle, and the sealing strip on the second baffle extends into the first baffle in the slot of the plate. 6. The easy-to-operate rearing bottle of Drosophila pupa parasitoid according to claim 4, characterized in that, a support body is fixed on the top of the conduit, and the rotating shaft is rotatably connected to the support body. 7. the rearing bottle of the easy-to-operate Drosophila pupa parasitoid according to claim 1, is characterized in that, the bee-blocking unit comprises the bottle cap threadedly connected with the bottle mouth, the hanging board that is arranged on the inside of the bottle cap, is arranged on the hanging plate The radial rods between the plate and the inner wall of the bottle cap, the gauze set between the adjacent radial rods and the inner wall of the bottle cap, and the sealing plate is screwed on the top of the bottle cap. 8. the rearing bottle of the Drosophila pupa parasitoid that is easy to operate according to claim 7, it is characterized in that, be provided with the limiting ring fixedly connected with the inner wall of the bottle cap above the radial rod, the limiting ring is used for and the sealing board contact. 9. The feeding bottle of the Drosophila pupa parasitoid that is easy to operate according to claim 7, wherein a hanging hole is provided in the center of the hanging plate, and the hanging hole is used for hanging a breeding bag. 10. the operation method of the feeding bottle of the Drosophila pupa parasitoid that is easy to operate, is characterized in that, may further comprise the steps: (1) hanging the breeding bag on the bottle cap of the first single bottle, and making the breeding bag placed in the first single bottle; (2) When most of the third instar larvae in the breeding bag in the first single bottle develop fruit fly pupae and adhere to the inner wall of the first single bottle, put the parasitic wasp into the second single bottle, and seal the first single bottle. The conduit at the right end of the bottle, open the conduits at the left end of the first single bottle, both ends of the second single bottle and both ends of the third single bottle, close the caps of the second single bottle and the third single bottle through the sealing plate, and then Blow air into the tube at the left end of the third single bottle, and the parasitic wasps in the second single bottle are transferred to the first single bottle; then, close the tube at the left end of the first single bottle; (3) Hang the breeding bag on the cap of the second single bottle, place the breeding bag in the second single bottle, remove the sealing plate on the cap of the second single bottle, and close the second single bottle conduits at both ends; (4) After the fruit fly pupae in the first single bottle are exhausted, install a sealing plate on the bottle cap of the first single bottle, remove the sealing plate on the bottle cap of the second single bottle, and open the second single bottle. Then, blow air into the tube at the right end of the first single bottle, transfer the parasitic wasps in the first single bottle to the second single bottle, and then close the tube at the right end of the second single bottle. , the parasitic wasps continue to breed in the second single bottle; (5) Hang the breeding bag on the bottle cap of the third single bottle, place the breeding bag in the third single bottle, remove the sealing plate on the bottle cap of the third single bottle, and close the third single bottle conduits at both ends; (6) After the fruit fly pupae in the second single bottle are exhausted, install a sealing plate on the bottle cap of the second single bottle, remove the sealing plate on the bottle cap of the third single bottle, and open the third single bottle. Then blow air into the tube at the right end of the first single bottle, transfer the parasitic wasps in the second single bottle to the third single bottle, and then close the tube at the right end of the third single bottle , at which time the parasitoid continued to breed in the third single bottle until maturity.

Images