CN101347184B - Semi-artificial cultivated diamondback moth feed and method of preparing the same - Google Patents
Semi-artificial cultivated diamondback moth feed and method of preparing the same Download PDFInfo
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- CN101347184B CN101347184B CN200810058886XA CN200810058886A CN101347184B CN 101347184 B CN101347184 B CN 101347184B CN 200810058886X A CN200810058886X A CN 200810058886XA CN 200810058886 A CN200810058886 A CN 200810058886A CN 101347184 B CN101347184 B CN 101347184B
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Abstract
The invention relates to a half artificially fed cabbage moth feed and a preparation method thereof. The feed is prepared by mixtures A, B and C, agar and distilled water, wherein the mixture A comprises casein, sucrose, malt meal and wild cabbage powder; the mixture B comprises cellulose, para hydroxybenzoic acid, potassium sorbate and Watkins salt; the mixture C comprises vitamin C, streptomycin sulphate, propyl gallate, vitamin B complex, edible vegetable oil, potassium hydroxide and formalin. The preparation method comprises the following steps: components of the mixture A, B and C are respectively put into a container; 5/6 of the distilled water and the agar powder are added into the container for heating and stirring to thawing; the mixture A is poured into the agar liquid and stirred until uniformity; the mixture B is added and stirred until uniformity; the rest distilled water is added, and the mixture C is added when the temperature is decreased to 70 DEG C, and then stirred until uniformity; the obtained product is filled into moth feeding cups; after the surface moisture is dried, the cups are put into a refrigerator for storage. The preparation method and the feed of the invention are scientific, simple, and easy to standardize propagation the cabbage moth indoors with large scale; the cabbage moth fed with the feed has high larva survival rate, orderly developmentand sound growth; and the descendants of the cabbage moth have high hatching rate.
Description
Technical field
The present invention relates to propagate artificially the feed of insect, concrete is semi-artificial cultivated diamondback moth feed and preparation method thereof.
Background technology
Diamondback moth Plutella xylostella (Linnaeus1758) was from whole so far 250 years of definite designation.
At present, diamondback moth is universal brassicaceous vegetable important pests, and according to the nineties document announcement in last century, the expense that the whole world is used to prevent and treat diamondback moth every year reaches 1,000,000,000 dollars, and only the U.S. is annual just expensive 100,000,000 dollars.
Biology and ecological characteristics such as diamondback moth has that reproductive capacity is strong, life cycle is short, the alternation of generations and adaptability are strong, and fast with its development of drug resistance to agricultural chemicals, resistance; Cause the chemical pesticide control difficulty big, cause in the chemical prevention process, the working concentration and the medication of agricultural chemicals frequently improve constantly; Formed the high chi in " agricultural chemicals-resistance-agricultural chemicals " road; The evil that the improves constantly circulation that evil spirit is high one zhang, and the major pest of the current vegetables that become also is to be studied proof up to now to nearly all agricultural chemicals; Comprise that biological pesticide BT has produced the insect of resistance, also be considered to the example of a relatively more typical failure of chemical pesticide control insect simultaneously.
Harm how economic, safe for seeking, that continue and control effectively diamondback moth, this patent applicant has introduced diamondback moth larvae advantage parasitic enemy insect---and curved tail ichneumon wasp is carried out diamondback moth biological control Study on Technology.
For enlarging the technological achievement of vegetable-crop pest-insect biological control; Carry out indoor batch and expand numerous curved tail ichneumon wasp; At first need regularly acquisition healthy diamondback moth in enormous quantities, this just needs artificial feeding diamondback moth in enormous quantities, is time in the indoor Brussels sprouts feeding process commonly used of solution diamondback moth and the disease and pest problem in space and the growing vegetables process; Man-made feeds breeding diamondback moth is an important channel, and the key issue of artificial feeding diamondback moth is the feed problem.When the prescription that uses other data to provide was cultivated diamondback moth, diamondback moth larvae was grown slowly usually, and development time reaches one month, and development duration is irregular, and is difficult to normally pupate and sprout wings.
Summary of the invention
The purpose of this patent invention is to provide a kind of semi-artificial cultivated diamondback moth feed and preparation method thereof.It is normal that this method can make diamondback moth larvae grow, and it is healthy to grow up, and normally pupates and sprouts wings, for diamondback moth provides in good time and wide variety of materials as the test insect with the numerous indoor host of natural enemy insect parasitic wasp expansion.
The technical scheme of this patent invention does, the feed of semi-artificial cultivated diamondback moth is characterized in that by mixture A, mixture B and mixture C and agar 80-120 gram, distilled water 2500-3500 milliliter preparation composition, wherein:
The composition of mixture A and weight portion are: casein 100-140, sucrose 110-150, malt flour 150-200, wild cabbage powder 50-150;
The composition of mixture B and weight portion are: cellulose 20-30, methyl p-hydroxybenzoate 4-6, potassium sorbate 3-5, Webster salt 20-50;
The composition of mixture C and consumption are: vitamin C 12-16, streptomycin sulphate 1-8, propyl group gallate 0.5-1.2, vitamin B compound 33-38, edible vegetable oil 20-50,45% potassium hydroxide 8-10,40% formalin 2-5, and wherein vitamin C, streptomycin sulphate, propyl group gallate, vitamin B compound are weight portion; Edible vegetable oil, 45% potassium hydroxide, 40% formalin are volume part.
The preparation method of semi-artificial cultivated diamondback moth feed is characterized in that following step preparation:
(1), presses the composition difference of mixture A, mixture B and mixture C group according to good each composition of prescription weighing
Put into three containers, each composition part is mixed to evenly, is mixture A, mixture B and mixture C;
(2) get distilled water total amount about 5/6ths and agar powder and add in the rustless steel container, heating is not higher than 90 ℃,
Be stirred to melt fully and be agar solution;
(3) mixture A is poured in the agar solution for preparing, be stirred to evenly;
(4) add mixture B, be stirred to evenly;
(5) add remaining distilled water, when cooling to 70 ℃;
(6) add mixture C, be stirred to evenly;
(7) with supporting the packing of worm cup, bowl cover is opened wide, put into refrigerator, 4 ℃ of stored refrigerated after drying surface moisture.Described malt flour and wild cabbage powder are bright article oven dry, grind, and cross 80 mesh sieves, with sieving dry powder down.
Diamondback moth meridic diet composition must comprise the growth course desired nutritional material of diamondback moth, and the basic biological characteristicses such as feeding habit that meet diamondback moth.The feed of half artificial feeding diamondback moth reasonable in design should possess, necessary nutrition and rational nutritive proportion; Stimulateing appetite arranged and help the composition that diamondback moth grows; The effective ingredient of preventing and curing diseases is arranged; Good antiseptic effect is arranged.Nutrient balance is the key problem of development feed, and the imbalance of feed nutrition composition will influence the normal growth of diamondback moth and grow.
Growth course of diamondback moth and life habit have uniqueness and the nutritional need of self, on nutriment kind and ratio, have significant difference with the insect of other artificial feeding.
The present invention is according to the physiological requirements of growing of diamondback moth, in feed formula reasonable disposition essential various nutritions and the corresponding proportion thereof of diamondback moth growth course.Because the specified temp and the humidity of diamondback moth larvae growth, niche is vulnerable to the pollution of various assorted bacterium and causes feed mold, and becoming influence diamondback moth and normally get and eat and paathogenic factor, even mortality.So, be furnished with the effective ingredients such as antibiotic of preventing and curing diseases in the feed formula.
The composition that the present invention is directed in the feed has been researched and developed specific preparation method, makes that the physical behavior and the chemical analysis of the effective ingredient in the feed are protected to greatest extent.
Feed formula of the present invention has the characteristics of nutrient balance to the diamondback moth whole growing, and the normal growth that is suitable for diamondback moth larvae is grown, and raw material conveniently is easy to get, and is cheap; Feed formula preparation method science, easy is prone to scale and the numerous diamondback moth of the indoor expansion of standardization; It is neat that the diamondback moth larvae of being raised has survival rate height, robust growth, growth, and the incubation rate for the diamondback moth ovum is high thereafter, raises generation surplus the diamondback moth 10 with the present invention, do not find degradation phenomena.
The specific embodiment
According to the form below is got the raw materials ready:
Wherein malt flour and wild cabbage powder are to be milled to 100 orders after bright Fructus Hordei Germinatus or the cabbage leaves oven dry.
The preparation method of the feed of semi-artificial cultivated diamondback moth is following step:
(1) according to good each composition of above-mentioned prescription weighing, by mixture A, mixture B, and mixture C composition put into three containers respectively, each is formed and part fully mixes, and becomes mixture A, mixture B and mixture C;
(2) distilled water and the agar powder of getting total amount about 5/6ths are put into rustless steel container, and heating is not higher than 90 ℃, are stirred well to melt fully to be agar solution;
(3) mixture A is poured in the agar solution for preparing, fully stir 30 seconds to even; Then add mixture B, fully stir 30 seconds, add remaining distilled water to evenly; When cooling to 70 ℃, add mixture C, stir 1 minute to even; With a cup packing, bowl cover is opened wide, can put into refrigerator after drying surperficial moisture content; 4 ℃ of stored refrigerated, the feed for semi-artificial cultivated diamondback moth takes out during use.
Through the test statistics analysis, with the diamondback moth larvae that feed formula of the present invention is raised, the survival rate 63.1 ± 2.4% of first-instar young, the percentage of pupation 56.4 ± 1.4% of four-age larva, the incubation rate of ovum are 55.5 ± 5.2%.Conclusion: the feed of production of the present invention can be used for indoor diamondback moth on a large scale and raise.
Claims (3)
1. semi-artificial cultivated diamondback moth feed is characterized in that distilled water 2500-3500 milliliter is processed by mixture A, mixture B and mixture C and agar 80-120 gram, wherein:
The composition of mixture A and weight are: casein 100-140 gram, sucrose 110-150 gram, malt flour 150-200 gram, wild cabbage powder 50-150 gram;
The composition of mixture B and weight are: cellulose 20-30 gram, methyl p-hydroxybenzoate 4-6 gram, potassium sorbate 3-5 gram, Webster salt 20-50 gram;
The composition of mixture C and consumption are: vitamin C 12-16 gram, streptomycin sulphate 1-8 gram, propyl group gallate 0.5-1.2 gram, vitamin B compound 33-38 gram, edible vegetable oil 20-50 milliliter, 45% potassium hydroxide 8-10 milliliter, 40% formalin 2-5 milliliter; Wherein vitamin C, streptomycin sulphate, propyl group gallate, vitamin B compound are weight; Edible vegetable oil, 45% potassium hydroxide, 40% formalin are volume.
2. the preparation method of semi-artificial cultivated diamondback moth feed as claimed in claim 1 is characterized in that by following step preparation:
(1) according to good each composition of prescription weighing, put into three containers respectively by the composition of mixture A, mixture B and mixture C group, each forms part abundant a mixing, becomes mixture A, mixture B and mixture C;
(2) get distilled water total amount about 5/6ths and agar powder and add in the rustless steel container, heating is not higher than 90 ℃, is stirred to melt fully to be agar solution;
(3) mixture A is poured in the agar solution for preparing, be stirred to evenly;
(4) add mixture B, be stirred to evenly;
(5) add remaining distilled water, when cooling to 70 ℃;
(6) add mixture C, be stirred to evenly;
(7) with supporting the packing of worm cup, bowl cover is opened wide, put into refrigerator, 4 ℃ of stored refrigerated after drying surface moisture.
3. like the preparation method of the said semi-artificial cultivated diamondback moth feed of claim 2, it is characterized in that described malt flour and wild cabbage powder are bright article oven dry, grind, and cross 80 mesh sieves, with sieving dry powder down.
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| CN200810058886XA CN101347184B (en) | 2008-09-09 | 2008-09-09 | Semi-artificial cultivated diamondback moth feed and method of preparing the same |
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| CN200810058886XA CN101347184B (en) | 2008-09-09 | 2008-09-09 | Semi-artificial cultivated diamondback moth feed and method of preparing the same |
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| CN101347184B true CN101347184B (en) | 2012-05-23 |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507472B (en) * | 2009-03-19 | 2011-09-07 | 中国农业科学院植物保护研究所 | Artificial feedstuff of striped rice borer, preparation method thereof and breeding method |
| CN101584420B (en) * | 2009-06-10 | 2011-11-23 | 中国热带农业科学院环境与植物保护研究所 | Brontispa longissima larva artificial feed and preparation method |
| CN101703172B (en) * | 2009-11-09 | 2012-05-23 | 中国热带农业科学院椰子研究所 | Semi-artificial feed for larva of rhynchophorus ferrugineus |
| CN101971931B (en) * | 2010-11-09 | 2012-07-04 | 中国农业科学院植物保护研究所 | Artificial feed of sesamia inferens walker as well as preparation method and feeding method thereof |
| CN102599388B (en) * | 2012-04-09 | 2013-04-10 | 天津师范大学 | Artificial feed for Zeuzera leuconotum and preparation method of the feed |
| CN102987159B (en) * | 2012-12-17 | 2014-10-29 | 湖北省农业科学院植保土肥研究所 | Semi-artificial diet for plutella xylostella larvae, and preparation method and application of diet |
| CN103478486A (en) * | 2013-09-11 | 2014-01-01 | 华南农业大学 | Diamondback moth larva pure artificial feed and preparation method thereof |
| CN103858827A (en) * | 2014-02-20 | 2014-06-18 | 云南省农业科学院农业环境资源研究所 | Method for carrying out indoor large-scale diadegma semiclausum hellen breeding |
| CN105010243B (en) * | 2015-07-28 | 2017-06-16 | 武汉科诺生物科技股份有限公司 | A kind of diamondback moth man-made feeds and diamondback moth indoors artificial method for breeding |
| CN105433065A (en) * | 2015-10-22 | 2016-03-30 | 延安大学 | Artificial compound feed for breeding adult Plutella xylostella and preparation method thereof |
| CN106106375B (en) * | 2016-06-30 | 2019-02-05 | 云南省农业科学院农业环境资源研究所 | A method of with mass rearing diamondback moth in water planting wild cabbage room |
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| CN1994241A (en) * | 2005-12-06 | 2007-07-11 | 中国林业科学研究院森林保护研究所 | American white moth artificial breeding and propagating method and artificial feed for larva thereof |
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Patent Citations (1)
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|---|---|---|---|---|
| CN1994241A (en) * | 2005-12-06 | 2007-07-11 | 中国林业科学研究院森林保护研究所 | American white moth artificial breeding and propagating method and artificial feed for larva thereof |
Non-Patent Citations (3)
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