CN105433065A - Artificial compound feed for breeding adult Plutella xylostella and preparation method thereof - Google Patents

Artificial compound feed for breeding adult Plutella xylostella and preparation method thereof Download PDF

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Publication number
CN105433065A
CN105433065A CN201510696195.2A CN201510696195A CN105433065A CN 105433065 A CN105433065 A CN 105433065A CN 201510696195 A CN201510696195 A CN 201510696195A CN 105433065 A CN105433065 A CN 105433065A
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China
Prior art keywords
compound feed
preparation
plutella xylostella
distilled water
adult
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CN201510696195.2A
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Chinese (zh)
Inventor
王秀康
邢英英
徐世才
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Yanan University
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Yanan University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention discloses an artificial compound feed for breeding adult Plutella xylostella. The artificial compound feed for breeding adult Plutella xylostella comprises the following ingredients in percentage by weight in per 100%: 1-2% of glucosinolate, 11-12% of jujube-blossom honey, 10-12% of sucrose, 11-13% of milk, 2.5-3.5% of yeast, 2-3% of royal jelly and distilled water that accounts for all the remaining percentages. The invention further discloses a preparation method of the artificial compound feed. The preparation method comprises the following steps: heating the distilled water to 60 DEG C; adding the milk and the sucrose into the heated distilled water; cooling the mixture to 45 DEG C; adding the jujube-blossom honey, the royal jelly and the yeast into the cooled mixture then uniformly mixing, so that a mixed solution is obtained; cooling the mixed solution to 30 DEG C, adding the glucosinolate into the cooled mixed solution, uniformly mixing then cooling the mixture, so that the artificial compound feed is prepared. The artificial compound feed for breeding adult Plutella xylostella disclosed by the invention is rich in various nutrients and is capable of increasing egg-laying amount of the adult Plutella xylostella as well as raising the hatching rate; and the larvae of the Plutella xylostella incubated from the eggs are uniform in instars. Moreover, the preparation method is simple, convenient to operate and easy to commercialize.

Description

A kind of artificial mixed feed cultivating diamondback moth adult and preparation method thereof
Technical field
The invention belongs to mixed feed technical field, be specifically related to a kind of artificial mixed feed cultivating diamondback moth adult, the invention still further relates to the preparation method of this mixed feed.
Background technology
Diamondback moth has another name called little blue or green worm, and being the important pests of the brassicaceous vegetables such as Chinese cabbage, rape, cauliflower, wild cabbage, is also a kind of insect widely distributed in the world.Because the reasons such as the heavy development of drug resistance of its harm is fast, high-output stress-resistance become the important experimental insect of the aspects such as research pest resistance to insecticide, physiology, pathology, toxicology.But the worm source ages gathered in field differ, cannot as supplying examination worm source.Just need to propagate artificially to obtain the neat experimental insect of a large amount of growth.At present, conventional adult breeding feed mainly contains glucose solution, honey solution.But the diamondback moth adult utilizing existing feed to cultivate exists growing way heterogeneity, egg laying amount is low, and incubation rate is lower, the not first-class problem of larval instar of hatching.And man-made feeds batching is many, process for preparation is still aobvious loaded down with trivial details, and not easily commercialization, applies not extensive.Therefore a kind of artificial mixed feed cultivating diamondback moth adult is made particularly important.
Summary of the invention
The object of this invention is to provide a kind of artificial mixed feed cultivating diamondback moth adult, solve egg laying amount when utilizing existing feed to carry out the cultivation of diamondback moth adult low, incubation rate is lower, the problem that the larva worm of hatching differs age.
Another object of the present invention is to provide a kind of preparation method cultivating the artificial mixed feed of diamondback moth adult.
The technical solution adopted in the present invention is, a kind of artificial mixed feed cultivating diamondback moth adult, according to mass percent, composed of the following components: glucosinolate 1 ~ 2%, jujube flower nectar 11 ~ 12%, sucrose 10 ~ 12%, milk 11 ~ 13%, yeast 2.5 ~ 3.5%, royal jelly 2 ~ 3%, all the other are distilled water, and above constituent mass percentage sum is 100%.
Another technical scheme of the present invention is, a kind of preparation method cultivating the artificial mixed feed of diamondback moth adult, specifically implements according to following steps:
Step 1, takes glucosinolate 1 ~ 2% by mass percentage respectively, jujube flower nectar 11 ~ 12%, sucrose 10 ~ 12%, milk 11 ~ 13%, yeast 2.5 ~ 3.5%, royal jelly 2 ~ 3%, and all the other are distilled water, and above constituent mass percentage sum is 100%;
Step 2, is heated to 60 DEG C by distilled water, add milk and sucrose stirring and dissolving wherein, after being cooled to 45 DEG C, then adds jujube flower nectar, royal jelly, yeast mixing, stirs, obtain mixed solution;
Step 3, when step 2 gained mixed solution is cooled to 30 DEG C, adds glucosinolate, stirs, cool and get final product.
The invention has the beneficial effects as follows, the present invention cultivates the artificial mixed feed of diamondback moth adult, is rich in multiple nutritional components, and utilize this feed to cultivate diamondback moth, diamondback moth Adult worms producting eggs amount increases, and incubation rate increases, and the diamondback moth larvae worm of hatching gained is neat for age; And drawing materials conveniently, preparation method is simple, easy and simple to handle, is easy to commercialization.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in detail.
The present invention cultivates the artificial mixed feed of diamondback moth adult, according to mass percent, composed of the following components: glucosinolate 1 ~ 2%, jujube flower nectar 11 ~ 12%, sucrose 10 ~ 12%, milk 11 ~ 13%, yeast 2.5 ~ 3.5%, royal jelly 2 ~ 3%, all the other are distilled water, and above constituent mass percentage sum is 100%.
The preparation method of above-mentioned artificial mixed feed, specifically implements according to following steps:
Step 1, takes glucosinolate 1 ~ 2% by mass percentage respectively, jujube flower nectar 11 ~ 12%, sucrose 10 ~ 12%, milk 11 ~ 13%, yeast 2.5 ~ 3.5%, royal jelly 2 ~ 3%, and all the other are distilled water, and above constituent mass percentage sum is 100%;
Step 2, is heated to 60 DEG C by distilled water, add milk and sucrose stirring and dissolving wherein, after being cooled to 45 DEG C, then adds jujube flower nectar, royal jelly, yeast mixing, stirs, obtain mixed solution;
Step 3, when step 2 gained mixed solution is cooled to 30 DEG C, adds glucosinolate, stirs, cool and get final product.
Feeding environment and method: use the dependent insect cage of 20 × 20 × 40cm to cultivate 50 pairs of diamondback moth adults, put into growth cabinet, temperature is 24 ~ 26 DEG C, relative humidity is 60 ~ 70%, infiltrate this feed 40ml with sponge or cotton, be put in dependent insect cage, 3h changes once.This experiment in triplicate.
The present invention is a kind of cultivates jujube flower nectar in the artificial mixed feed of diamondback moth adult, sucrose, and milk, containing abundant nutrition, can provide nutritional need for the growth of diamondback moth adult; Mustard oil wherein originates in crucifer, and diamondback moth has chemotaxis to mustard oil, adds mustard oil and diamondback moth can be attracted to take food; Add yeast, can fermentation protein, high molecular weight protein in milk and jujube flower nectar is converted into small-molecular peptides, takes food for diamondback moth; Wherein royal jelly nutritive enriches, nutriment is planted containing royal jelly acid, protein, amino acid, aliphatic acid, vitamin, choline, mineral matter, nucleic acid and nucleotides etc. more than 30, wherein royal jelly acid is most important one, there is the function of well antibacterial and reinforced immunological power, the immunity of diamondback moth can be strengthened.Utilize this feed to cultivate diamondback moth, diamondback moth Adult worms producting eggs amount increases, and incubation rate increases, and the diamondback moth larvae worm of hatching gained is neat for age;
Embodiment 1
Get 60mL distilled water and be heated to 60 DEG C, add 11g milk and 12g sucrose wherein, stirring and dissolving is molten be cooled to 45 DEG C after, then add 11g jujube flower nectar, 2g royal jelly, 3g yeast, mixing and stirring, obtains mixed solution; Until mixed solution be cooled to 30 DEG C time, add 1g glucosinolate, stir, cool and get final product.
Embodiment 2
Get 57.5mL distilled water and be heated to 60 DEG C, add 13g milk and 10g sucrose stirring and dissolving wherein, after being cooled to 45 DEG C, then add 11g jujube flower nectar, 3g royal jelly, the mixing of 3.5g yeast, stir, obtain mixed solution; Until mixed solution be cooled to 30 DEG C time, add 2g glucosinolate, stir, cool and get final product.
Embodiment 3
Get 58.5mL distilled water and be heated to 60 DEG C, add 12g milk and 11g sucrose stirring and dissolving wherein, after being cooled to 45 DEG C, then add 12g jujube flower nectar, 2g royal jelly, the mixing of 2.5g yeast, stir, obtain mixed solution; Until mixed solution be cooled to 30 DEG C time, add 2g glucosinolate, stir, cool and get final product.
Utilize the artificial mixed feed of the embodiment of the present invention 2 gained (A), 10% Glucose Liquid (B) carries out diamondback moth imago breeding respectively under same environmental condition, its egg laying amount and incubation rate are as following table:
As can be seen from the table, when supplying examination borer population, rearing conditions identical, the diamondback moth Adult worms producting eggs amount of the artificial mixed feed cultivation of diamondback moth adult and incubation rate are all higher than 10% Glucose Liquid, the present invention cultivates the artificial mixed feed of diamondback moth adult, be rich in multiple nutritional components, and draw materials conveniently, preparation method is simple, easy and simple to handle, be easy to commercialization.

Claims (2)

1. one kind cultivates the artificial mixed feed of diamondback moth adult, it is characterized in that, according to mass percent, composed of the following components: to get glucosinolate 1 ~ 2%, jujube flower nectar 11 ~ 12%, sucrose 10 ~ 12%, milk 11 ~ 13%, yeast 2.5 ~ 3.5%, royal jelly 2 ~ 3%, all the other are distilled water, and above constituent mass percentage sum is 100%.
2. cultivate a preparation method for the artificial mixed feed of diamondback moth adult, it is characterized in that, specifically implement according to following steps:
Step 1, takes respectively by mass percentage and gets glucosinolate 1 ~ 2%, jujube flower nectar 11 ~ 12%, sucrose 10 ~ 12%, milk 11 ~ 13%, yeast 2.5 ~ 3.5%, royal jelly 2 ~ 3%, and all the other are distilled water, and above constituent mass percentage sum is 100%;
Step 2, is heated to 60 DEG C by distilled water, add milk and sucrose stirring and dissolving wherein, after being cooled to 45 DEG C, then adds jujube flower nectar, royal jelly, yeast mixing, stirs, obtain mixed solution;
Step 3, when step 2 gained mixed solution is cooled to 30 DEG C, adds glucosinolate, stirs, cool and get final product.
CN201510696195.2A 2015-10-22 2015-10-22 Artificial compound feed for breeding adult Plutella xylostella and preparation method thereof Pending CN105433065A (en)

Priority Applications (1)

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CN201510696195.2A CN105433065A (en) 2015-10-22 2015-10-22 Artificial compound feed for breeding adult Plutella xylostella and preparation method thereof

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101347184A (en) * 2008-09-09 2009-01-21 云南省农业科学院农业环境资源研究所 Semi-artificial cultivated diamondback moth feed and method of preparing the same
CN102125184A (en) * 2011-01-18 2011-07-20 武汉武大绿洲生物技术有限公司 Artificial feed for larvae of plutella xylostella and preparation method thereof
CN103380860A (en) * 2013-07-05 2013-11-06 延安大学 Artificial mixed feed used for culturing Buthus martensii Karsch
CN104351574A (en) * 2014-11-28 2015-02-18 延安大学 Artificial compound feed for breeding sericinus montelus and preparation method thereof
CN104814352A (en) * 2015-05-20 2015-08-05 何文雅 Tenebrio molitor adult feed

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101347184A (en) * 2008-09-09 2009-01-21 云南省农业科学院农业环境资源研究所 Semi-artificial cultivated diamondback moth feed and method of preparing the same
CN102125184A (en) * 2011-01-18 2011-07-20 武汉武大绿洲生物技术有限公司 Artificial feed for larvae of plutella xylostella and preparation method thereof
CN103380860A (en) * 2013-07-05 2013-11-06 延安大学 Artificial mixed feed used for culturing Buthus martensii Karsch
CN104351574A (en) * 2014-11-28 2015-02-18 延安大学 Artificial compound feed for breeding sericinus montelus and preparation method thereof
CN104814352A (en) * 2015-05-20 2015-08-05 何文雅 Tenebrio molitor adult feed

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
娄国强等: "《昆虫研究技术》", 31 October 2006, 西南交通大学出版社 *

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Application publication date: 20160330