CN114916505B - Population continuation research method for biodegradable farmland residual films of Chilo suppressalis - Google Patents
Population continuation research method for biodegradable farmland residual films of Chilo suppressalis Download PDFInfo
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- CN114916505B CN114916505B CN202210706023.9A CN202210706023A CN114916505B CN 114916505 B CN114916505 B CN 114916505B CN 202210706023 A CN202210706023 A CN 202210706023A CN 114916505 B CN114916505 B CN 114916505B
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- 241000426497 Chilo suppressalis Species 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 53
- 238000011160 research Methods 0.000 title claims abstract description 20
- 230000012447 hatching Effects 0.000 claims abstract description 43
- 238000011282 treatment Methods 0.000 claims abstract description 43
- 239000004698 Polyethylene Substances 0.000 claims abstract description 32
- -1 polyethylene Polymers 0.000 claims abstract description 25
- 229920000573 polyethylene Polymers 0.000 claims abstract description 25
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 22
- 239000000463 material Substances 0.000 claims abstract description 13
- 230000037406 food intake Effects 0.000 claims abstract description 7
- 241000382353 Pupa Species 0.000 claims description 51
- 235000013601 eggs Nutrition 0.000 claims description 27
- 238000012360 testing method Methods 0.000 claims description 23
- 241000238631 Hexapoda Species 0.000 claims description 22
- 230000017448 oviposition Effects 0.000 claims description 17
- 238000012258 culturing Methods 0.000 claims description 12
- 230000037396 body weight Effects 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 9
- 235000013305 food Nutrition 0.000 claims description 7
- 238000003973 irrigation Methods 0.000 claims description 7
- 230000002262 irrigation Effects 0.000 claims description 7
- 230000019617 pupation Effects 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000000645 desinfectant Substances 0.000 claims description 5
- 230000013011 mating Effects 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000009395 breeding Methods 0.000 claims description 4
- 230000001488 breeding effect Effects 0.000 claims description 4
- 230000007850 degeneration Effects 0.000 claims description 4
- 230000032669 eclosion Effects 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 239000002985 plastic film Substances 0.000 claims 2
- 229920006255 plastic film Polymers 0.000 claims 2
- 230000000593 degrading effect Effects 0.000 claims 1
- 235000013339 cereals Nutrition 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 5
- 235000012631 food intake Nutrition 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000001993 wax Substances 0.000 description 33
- 229920003023 plastic Polymers 0.000 description 11
- 239000004033 plastic Substances 0.000 description 9
- 239000000843 powder Substances 0.000 description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
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- 238000004458 analytical method Methods 0.000 description 4
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- 235000021050 feed intake Nutrition 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 235000013871 bee wax Nutrition 0.000 description 2
- 239000012166 beeswax Substances 0.000 description 2
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- 238000010832 independent-sample T-test Methods 0.000 description 2
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- 230000007774 longterm Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
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- 241000894006 Bacteria Species 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009341 apiculture Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 239000007766 cera flava Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
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- 230000003071 parasitic effect Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a population continuation research method of a large wax moth biodegradable farmland residual film, which comprises the steps of under the condition of artificial feed feeding, ensuring that the spawning quantity of female moths is highest at 25-35 ℃ and 30 ℃ to reach 1139 grains/female moths; 64 In a cm 3 hatching bottle, the hatching rate of the larvae of the Chilo suppressalis is highest and is up to 74.17% at 80 grains within the range of 40-120 grains. Namely, the temperature is 30 ℃ and the egg density is 80, which is the optimal condition for continuing the population of the Chilo suppressalis. The second generation of food intake of the wax moth treated by different polyethylene materials is higher than that of the first generation, which is beneficial to population continuation, and the farmland residual film is polyethylene treatment with the best effect from the viewpoint of spawning amount. Under the condition of feeding the Xinjiang farmland residual film, the highest feeding amount of the large wax moth is 100% of Xinjiang farmland residual film treatment, and the highest spawning amount and hatching rate of wheat bran treatment. The invention verifies the feasibility of large-scale application and popularization of the large wax moth biodegradable farmland residual film under the environment-controllable condition.
Description
Technical Field
The invention relates to the technical field of population continuation of Chilo suppressalis, in particular to a population continuation research method for biodegradable farmland residual films of Chilo suppressalis.
Background
Residual film pollution is a worldwide problem, and the traditional treatment measures of landfill, incineration, chemical treatment and recycling after manual and mechanical recovery have high cost and cause secondary pollution to the environment, and biodegradation tends to be great.
The large wax moth is a main pest of the beekeeping industry, but has the advantages of rich protein content, short growth period, rich foodstuff sources, edibility, feeding and the like, the larva of the large wax moth is usually used as an infection model for researching the toxicity, pathogenicity and interaction of bacteria and a host, is also usually used for trapping and breeding nematodes and researching the propagation of parasitic bees, and the large wax moth biodegradable polyethylene plastic also becomes a research hotspot in recent years. The influence of environmental factors on growth and reproduction of large wax moth fed in laboratory scale is very important, and related researches such as: the method which is easy for laboratory annual batch breeding of the Chilo suppressalis is explored; research on environmental factors such as temperature, humidity range, illumination condition and the like of artificial feeding of the Chilo suppressalis is carried out; research on artificial feed formula of Chilo suppressalis is carried out in terms of material drawing, price and the like. However, the specific influence research on the growth and reproduction of the Chilo suppressalis by environmental factors such as temperature and density under indoor culture conditions in the related reports is not strong.
At present, the research of the polyethylene plastic degraded by the large wax moth is mostly biased to the degradation of the polyethylene plastic by intestinal microorganisms, namely, the capability of the polyethylene plastic degraded by the large wax moth is verified by extracting bacterial strains in the large wax moth, and the large wax moth can degrade the polyethylene plastic by observing large wax moth faeces microscopy, thermal capacity analysis and other methods of feeding the polyethylene plastic. The influence of the feeding of polyethylene plastic directly by the Chilo suppressalis on the growth of the Chilo suppressalis is focused on the influence on the contemporary growth of the Chilo suppressalis, and the continuous research of the feeding of polyethylene material on the Chilo suppressalis population is less.
In conclusion, the invention designs a population continuation research method for the biodegradable farmland residual film of the Chilo suppressalis.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention aims to provide a population continuation research method for the biodegradable farmland residual film of the Chilo suppressalis, which is carried out under the environment-controllable condition, and is used for researching the influence of temperature and density on the continuation of the Chilo suppressalis population under the artificial feed feeding condition, the influence of different food (polyethylene material) feeding conditions and Xinjiang farmland residual film feeding conditions on the continuation of the Chilo suppressalis population, thereby providing a certain reference basis for the development and utilization of the Chilo suppressalis insect resource.
In order to achieve the above object, the present invention is realized by the following technical scheme: a population continuation research method for biodegradable farmland residual films of Chilo suppressalis comprises the following steps:
1. Cleaning and sterilizing a feeding environment: the indoor floor is sterilized by 84 sterilizing liquid in a ratio of 1:500, walls, windows, test tables and the like around the incubator are sterilized by 84 sterilizing liquid in a ratio of 1:100, and the incubator, the insect culturing box, the tweezers and the like are sterilized by 75% alcohol solution.
2. Influence of temperature on growth and reproduction of Chilo suppressalis: different temperature gradients (25, 30, 35 ℃) and humidity of 60% + -10% RH (relative humidity) were set, and the wax moth was raised under dark room conditions for 1 cycle (egg-larva-pupa-adult-egg). Eggs (density 40, 60, 80, 100 and 120) are put into a hatching bottle and raised for 30d (enough artificial feed is ensured), larvae with weight of 0.1000-0.2000g (the sizes of larvae used in experiments are similar and experimental errors are reduced) are picked out, respectively put into an insect raising box and 10 heads of each box with 10.0000g artificial feed, after pupation (mature larvae are cocoons) are formed, the pupa is taken out in time and put into the insect raising box, after eclosion, female and male adults are put into the egg laying boxes with egg laying paper sheets in pairs, the adult period is not fed due to degeneration of the siphon mouth parts, and after mating and egg laying of the adults, the statistical egg laying amount of the egg sheets is taken out. The whole process is observed and recorded for each stage calendar period, the hatching rate is calculated, and the larva weight and body length change, the feeding amount, the pupa weight, the pupa length, the number of male and female adults, the spawning amount, the adult weight, the body length and the like are measured regularly. And 3 times of treatment, and comprehensively analyzing the influence of the temperature on the growth and reproduction of the Chilo suppressalis.
3. Effects of density on growth and propagation of Chilo suppressalis: putting 40, 60, 80, 100 and 120 eggs into transparent plastic hatching bottles of 64cm 3 respectively, adding sufficient artificial feed, repeating for 3 times each treatment, putting into incubator of 25, 30 and 35 ℃ respectively, keeping humidity at 60% +/-10% RH, culturing under darkroom condition, observing and recording daily, culturing for 30 days, counting the numbers of larvae of <0.1000g, 0.1000-0.2000g and >0.2000g in hatching bottles of different densities, calculating hatching rate, and comprehensively analyzing the influence of egg density on growth and reproduction of Chilo suppressalis.
4. The measuring method comprises the following steps: ① The method for measuring the body length of the larva of the Chilo suppressalis comprises the following steps: carefully clamping 1 head of larva of Chilo suppressalis on a test table by using forceps, and measuring the length of the larva of Chilo suppressalis in sequence when the body of the larva of Chilo suppressalis linearly moves and stretches; ② The method for measuring the pupa weight and the pupa length comprises the following steps: observing twice daily (10:00, 18:00) at regular time, measuring pupa weight and pupa length (weight and length of cocoons) when pupa appear, and separating to another insect-raising box; ③ Adult body weight and length measuring method: and (5) baking at 70 ℃ for 20min after spawning is finished, and weighing the dead body weight and the dead body length.
5. The test data are processed by Excel97-2003, and are subjected to SPSS 21 statistical analysis, and the significance statistics among the data are subjected to LSD method single-factor variance analysis.
6. Analysis of experimental results: the influence of temperature on growth and reproduction of the wax moth is mainly manifested in calendar, larva feeding capacity, weight and length of the wax moth, spawning amount, hatching rate and the like. 25. The generation period and the calendar period of the wax moth show a decreasing trend along with the temperature rise at 30 and 35 ℃, the higher the temperature is, the larger the feeding capacity, the weight and the body length (larva, pupa and adult) and the hatching rate of the wax moth are, the highest the spawning amount at 30 ℃ is, and the 1139 grains/female moth is reached.
The influence of density on growth and reproduction of the wax moth is mainly reflected in the aspects of larva weight and egg hatching rate. Under the conditions of a 64cm 3 hatching bottle and sufficient feed, at the same temperature, the egg density is 40, 60, 80, 100 and 120 grains, the number of larvae in weight sections of less than 0.1000g increases with the increase of the density, the number of larvae in weight sections of 0.1000-0.2000g is more when the densities 60 and 80 are treated, and the number of larvae in weight sections of more than 0.2000g decreases with the increase of the density; the hatching rate is highest and reaches 74.17% at 80 egg grain number.
The invention has the beneficial effects that:
the treatment of the invention affects the population continuation by affecting the feed intake, the body weight and length, the spawning amount and the hatching rate of the Chilo suppressalis. Provides a certain reference basis for the development and utilization of wax moth insect resources.
Detailed Description
The invention is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
Example 1: a population continuation research method of a large wax moth biodegradable farmland residual film (the continuous influence of temperature and density on the large wax moth population under the condition of artificial feed feeding) comprises the following steps:
1. instrument and apparatus: constant temperature incubator (Tianjin Test instruments Co., ltd DH6000A type), hygrothermograph (Anyi G-337), ten-thousandth electronic balance (Metrer-Tolyduo instruments (Shanghai Co., ltd., AL 104)), vernier caliper (precision 0.02 mm), plastic insect-raising box (length width 9.5X19.5X15.5 cm 3, evenly distributed ventilation holes on box cover), plastic hatching bottle (cylindrical, volume 64cm 3), magnifying glass (magnification 100, shanghai Lao Fuxiang optical instruments Co., ltd.), tweezers, etc.
2. Feed and insect source
The feed is artificial feed, and the formula comprises 1 part of milk powder, flour, wheat bran, beeswax, yeast powder, bean powder and honey, and 0.6 part of glycerol and corn powder. The preparation method comprises mixing milk powder, flour, testa Tritici, bean flour, semen Maydis powder and yeast powder, adding glycerol and Mel, mixing, adding heated and melted Cera flava, mixing, and refrigerating in refrigerator.
The tested insect source is from Shanxi university forest college laboratory, and the population is established in 2017, and is fed in an incubator by artificial feed (formula: milk powder 50g, yeast powder 20g, flour 100g, wheat bran 100g, corn flour 100g, beeswax 50g, honey 50g and glycerin 60 g), at 30.0+/-0.5 ℃ and humidity of 75.0+/-5.0%RH, and fed in dark.
3. The test method comprises the following steps:
(1) Cleaning and sterilizing a feeding environment: 84 disinfectant for indoor floor surface 1:500, wall, window, test bench around incubator, etc. 1:100, and an incubator, an insect-raising box, tweezers and the like are sterilized with a 75% alcohol solution.
(2) Test time and place: in 2021, a laboratory of a institute of soil fertilizer and agricultural water conservation;
(3) Influence of temperature on growth and reproduction of Chilo suppressalis: different temperature gradients (25, 30, 35 ℃) and humidity of 60% + -10% RH (relative humidity) were set, and the wax moth was raised under dark room conditions for 1 cycle (egg-larva-pupa-adult-egg). Eggs (density 40, 60, 80, 100 and 120) are put into a hatching bottle and raised for 30d (enough artificial feed is ensured), larvae with weight of 0.1000-0.2000g (the sizes of larvae used in experiments are similar and experimental errors are reduced) are picked out, respectively put into an insect raising box and 10 heads of each box with 10.0000g artificial feed, after pupation (mature larvae are cocoons) are formed, the pupa is taken out in time and put into the insect raising box, after eclosion, female and male adults are put into the egg laying boxes with egg laying paper sheets in pairs, the adult period is not fed due to degeneration of the siphon mouth parts, and after mating and egg laying of the adults, the statistical egg laying amount of the egg sheets is taken out. The whole process is observed and recorded for each stage calendar period, the hatching rate is calculated, and the larva weight and body length change, the feeding amount, the pupa weight, the pupa length, the number of male and female adults, the spawning amount, the adult weight, the body length and the like are measured regularly. And 3 times of treatment, and comprehensively analyzing the influence of the temperature on the growth and reproduction of the Chilo suppressalis.
(4) Effects of density on growth and propagation of Chilo suppressalis: putting 40, 60, 80, 100 and 120 eggs into transparent plastic hatching bottles respectively, adding enough artificial feed, putting the eggs into incubator at 25, 30 and 35 ℃ respectively for 3 times of treatment, keeping humidity of 60% +/-10% RH, keeping darkroom condition, observing and recording every day, keeping for 30 days, counting the numbers of larvae with the density of <0.1000g, 0.1000-0.2000g and >0.2000g in hatching bottles, calculating hatching rate, and comprehensively analyzing the influence of egg density on growth and reproduction of wax moth.
(5) The measuring method comprises the following steps: ① The method for measuring the body length of the larva of the Chilo suppressalis comprises the following steps: carefully clamping 1 head of larva of Chilo suppressalis on a test table by using forceps, and measuring the length of the larva of Chilo suppressalis in sequence when the body of the larva of Chilo suppressalis linearly moves and stretches; ② The method for measuring the pupa weight and the pupa length comprises the following steps: observing twice daily (10:00, 18:00) at regular time, measuring pupa weight and pupa length (weight and length of cocoons) when pupa appear, and separating to another insect-raising box; ③ Adult body weight and length measuring method: and (5) baking at 70 ℃ for 20min after spawning is finished, and weighing the dead body weight and the dead body length.
4. Data processing
The test data are processed by Excel97-2003, and are subjected to SPSS 21 statistical analysis, and the significance statistics among the data are subjected to LSD method single-factor variance analysis.
5. Test results:
The influence of temperature on growth and reproduction of the wax moth is mainly manifested in calendar, larva feeding capacity, weight and length of the wax moth, spawning amount, hatching rate and the like. 25. The generation period and the calendar period of the wax moth show a decreasing trend along with the temperature rise at 30 and 35 ℃, the higher the temperature is, the larger the feeding capacity, the weight and the body length (larva, pupa and adult) and the hatching rate of the wax moth are, the highest the spawning amount at 30 ℃ is, and the 1139 grains/female moth is reached.
The influence of density on growth and reproduction of the wax moth is mainly reflected in the aspects of larva weight and egg hatching rate. Under the conditions of a 64cm < 3 > hatching bottle and sufficient feed, at the same temperature, the egg density is 40, 60, 80, 100 and 120 grains, the number of larvae in weight sections of less than 0.1000g increases with the increase of the density, the number of larvae in weight sections of 0.1000-0.2000g is more when the densities 60 and 80 are treated, and the number of larvae in weight sections of more than 0.2000g decreases with the increase of the density; the hatching rate is highest and reaches 74.17% at 80 egg grain number.
Example 2: a population continuation research method for biodegradable farmland residual films of Chilo suppressalis (the influence of feeding of different polyethylenes on population continuation of the Chilo suppressalis) comprises the following steps:
1. instrument and apparatus: as in example 1;
2. Feed and insect source:
Feed: artificial feed, degradable film new film, PE new film, farmland residual drip irrigation belt and farmland residual film.
Preparing artificial feed: as in example 1;
The farmland residual film and the farmland residual drip irrigation belt are collected from a national desert soil fertility and fertilizer long-term monitoring station, wherein the farmland residual drip irrigation belt is the residue of the recovered farmland soil; the PE new film and the degradable film are produced by Xinjiang Uruku Jikuhui Limited and Xinjiang Kang Runjie environmental protection technology Co., ltd respectively.
Test insect source: as in example 1.
3. The test method comprises the following steps:
(1) Cleaning and sterilizing a feeding environment: as in example 1;
(2) Test time and place: as in example 1;
(3) The test process and method are as follows: under indoor constant temperature, constant humidity and darkroom conditions, namely 30 ℃ and 60% +/-10% RH (relative humidity), different polyethylene materials (degradable film new film, PE new film, DGD and farmland residual film) are used for artificially feeding the Chilo suppressalis for 2 generations, and each generation of experience stage is egg-larva-pupa-adult-egg, and the specific operation is as follows: putting 80 eggs in a hatching bottle of 64cm 3 for 30d (ensuring sufficient artificial feed), picking up larvae with the weight of 0.1000-0.2000g (ensuring the sizes of larvae used in experiments are similar and experimental errors are reduced) and putting into an empty insect-culturing box for 24h (aiming at leading the larvae to excrete the excrement of the early-stage feeding artificial feed and ensuring the accuracy of later-stage experiments), respectively putting into an insect-culturing box and 10 heads of each box which are added with 2.0000g of different polyethylene materials and 10.0000g of artificial feed, taking out the pupae in time after the pupae are formed (mature larvae are formed into cocoons), putting the pupae into the insect-culturing box alone, and putting female and male adults into the egg-culturing box with egg-culturing paper sheets in pairs after the eclosion of the pupae, wherein the adult is not fed in the adult stage and the egg-culturing sheets are taken out after the adult mating egg-culturing because of the degeneration of a siphon mouth. The whole process is observed and recorded for calendar of each stage, and the body weight and length changes, the feeding amount, the pupa weight, the pupa length, the spawning amount, the hatching rate, the adult weight, the body length and the like of the larvae are counted. Then the spawning at the same period is fed to the next generation according to the same method. And 3 times of treatment, comprehensively analyzing the influence of different foods on the continuation of the large wax moth population.
(4) The measuring method comprises the following steps: as in example 1;
4. and (3) data processing: test data were processed and analyzed using Excel 97-2003, origin2018 and SPSS 21, and data were compared using the LSD method and the independent sample T test method.
5. Test results:
The continuous influence of different foods on the population of the Chilo suppressalis is mainly expressed in aspects of feed intake, cocoon yield, pupa weight and pupa length, adult weight and length, spawning amount, hatching rate and the like: the feeding rate of different foods for feeding the Chilo suppressalis is obviously higher than that of different polyethylene materials, namely, the feeding rate, the pupa weight and the pupa length, the adult weight and the egg laying rate of the artificial feed are respectively 25.86-239.87 times (feeding rate), 2.07-3.75 times (pupa weight), 0.20-0.76 times (pupa length), 0.57-0.87 times (adult weight), 0.12-0.46 times (adult length), 0.33-0.54 times (egg laying rate) and 0.60-0.87 times (hatching rate). The food intake of the second generation of the treatment of different polyethylenes is increased compared with that of the first generation, wherein the food intake of the degradation film new film, the PE new film, the farmland residual drip irrigation tape and the farmland residual film is respectively 0.73 times, 2.16 times, 0.88 times and 1.80 times higher than that of the first generation, and the pupal weight, the pupal length, the adult weight and the egg hatching rate are lower than those of the second generation. The spawning amount is sequentially from high to low of farmland residual film, farmland residual drip irrigation tape, PE new film and degradable film new film. The cocoon setting rate of artificial feed is more than or equal to 90%, and the cocoon setting rate of different polyethylene treatments is 40% -85%. The weight of 0.1000-0.2000g of larva of Chilo suppressalis has the tendency of reducing the total calendar period when being treated by different polyethylenes compared with the artificial feed under different food conditions.
Example 3: a population continuation research method of a large wax moth biodegradable farmland residual film (population continuation research of the large wax moth under the condition of feeding the Xinjiang farmland residual film) comprises the following steps:
1. instrument and apparatus: as in example 1;
2. Feed and insect source:
Feeding raw materials: farmland residual film (Xinjiang farmland residual film), wheat bran;
the farmland residual film is collected from a national grayish-desert soil fertility and fertilizer long-term monitoring station; wheat bran was purchased from a grain and oil store in wuluzhuiqi, xinjiang.
The artificial feed formulation was the same as in example 1.
Test insect source: as in example 1.
3 Test method
(1) Cleaning and sterilizing a feeding environment: as in example 1;
(2) Test time and place: as in example 1;
(3) The test process and method are as follows: under indoor constant temperature, constant humidity and darkroom conditions, namely 30 ℃ and 60% +/-10% RH (relative humidity), mixing wheat bran and Xinjiang farmland residual film according to different proportions, artificially feeding Chilo suppressalis for 2 generations, wherein each generation of experience stage is egg-larva-pupa-adult-egg, and the specific operation is as follows: putting 80 eggs in a hatching bottle of 64cm 3 for 30d (ensuring sufficient artificial feed), picking up larvae with the weight of 0.1000-0.2000g (ensuring the sizes of larvae used in experiments to be similar and reducing experimental errors) into an empty larva raising box for 24h (ensuring the accuracy of later experiments), respectively putting into larva raising boxes with 5.0000g wheat bran (wheat bran treatment), 2.5000g wheat bran+ 2.5000g farmland residual film (50% farmland residual film treatment) and 5.0000g farmland residual film (100% farmland residual film treatment), taking out pupation (mature larva cocooning is pupation) after the pupation is formed, putting female and male adults into egg boxes with egg laying paper sheets in pairs after the pupation, and taking out egg pieces after the adult mating egg laying. The whole process is observed and recorded for calendar of each stage, and the body weight and length changes, the feeding amount, the pupa weight, the pupa length, the spawning amount, the hatching rate, the adult weight, the body length and the like of the larvae are counted. Then the spawning at the same period is fed to the next generation according to the same method. And 3 times of treatment, comprehensively analyzing the influence of the ingestion of the Xinjiang farmland residual film on the continuation of the Chilo suppressalis population.
(4) The measuring method comprises the following steps: as in example 1.
4. Data processing
Test data were processed and analyzed using Excel 97-2003 and SPSS 21, and data were compared using the LSD method and the independent sample T test method.
5. Test results:
Feeding weight segments of 0.1000-0.2000g of larva of Chilo suppressalis by using different proportions of Xinjiang farmland residual film and wheat bran (auxiliary nutrition), wherein the feeding wheat bran amount of larva of Chilo suppressalis is reduced (P < 0.05) along with the increase of the residual film adding amount, the feeding residual film amount is increased along with the increase of the residual film adding amount, the feeding wheat bran amount of each second generation is reduced compared with the first generation (wheat bran treatment is reduced by 0.15 times, 50% farmland residual film treatment is reduced by 0.29 times), and the feeding residual film amount is increased compared with the first generation (50% farmland residual film treatment is increased by 2.7 times and 100% farmland residual film treatment is increased by 3 times); the pupa weight and the pupa length of the large wax moth are reduced along with the increase of the residual film quantity, the pupa weight and the pupa length of 3 second generation are lower than those of the first generation (wheat bran, 50% farmland residual film and 100% farmland residual film are respectively lower than those of the first generation by 11.60%, 39.81% and 14.62%, and the pupa length is respectively lower than those of the first generation by 15.27%, 20.53% and 5.78%); the weight of male and female moths in different treatments is not obviously influenced by the weight of the male and female moths in different treatments, the weight of the male and female moths in different treatments is lower than that in the first generation (the weight of the female moths in the second generation is reduced by 22.88 percent, 23.85 percent and 11.63 percent compared with that in the first generation after the treatment of wheat bran, 50 percent of farmland residual film and 100 percent of farmland residual film, and the weight of the male and female moths in the second generation is reduced by 23.84 percent, 30.16 percent and 5.98 percent compared with that in the first generation, and the body length of the male and female moths in different treatments has no obvious change rule; the spawning amount of the wax moth is reduced along with the increase of the residual film amount, the spawning amount of the second generation of 100 percent farmland residual film treatment is increased by 0.23 percent (P is more than 0.05) compared with that of the first generation, and the spawning amount of the second generation of wheat bran and 50 percent farmland residual film treatment is less than that of the first generation (46.92 percent and 37.23 percent respectively) (P is less than 0.05); the hatching rate of the large wax moth eggs is reduced (P is more than 0.05) along with the increase of the addition of the farmland residual film, and the hatching rate of the second generation is smaller than that of the first generation (wheat bran, 50% farmland residual film and 100% farmland residual film treatment are respectively 10.35%, 5.20% and 9.27% smaller than those of the first generation) (P is more than 0.05).
In summary, the experiments of the present invention lead to the following conclusions:
(1) Under the condition of artificial feed feeding, the oviposition amount of female moths is highest at the temperature of 30 ℃ within the range of 25-35 ℃, and the oviposition amount reaches 1139 grains/female moths; in a 64cm 3 hatching bottle, the hatching rate of the larvae of the Chilo suppressalis is highest and is up to 74.17% at 80 grains within the range of 40-120 grains. Namely, the temperature is 30 ℃ and the egg density is 80, which is the optimal condition for continuing the population of the Chilo suppressalis.
(2) Under different food feeding conditions, the feeding amount, spawning amount and hatching rate of the larvae of the Chilo suppressalis treated by the artificial feed are all obviously higher than those of the larvae treated by different polyethylene materials; the food intake of the second generation artificial feed treatment is reduced compared with that of the first generation artificial feed treatment, and the food intake of the second generation artificial feed treatment is higher than that of the first generation artificial feed treatment; the hatching rate of the second generation of the treatment of different polyethylene materials is slightly lower than that of the first generation, and the treatment of different polyethylene materials sequentially comprises the treatment of farmland residual films, farmland residual drip irrigation belts, PE new films and degradable films from high to low. The second generation of feed intake of the wax moth treated by different polyethylene materials is higher than that of the first generation, which is beneficial to population continuation, and the artificial feed is polyethylene treatment with the best effect from the viewpoint of spawning amount.
(3) Under the condition of feeding Xinjiang artificial feed (different proportions of wheat bran and farmland residual film), the highest feeding amount of the large wax moth is 100% farmland residual film treatment, and the highest spawning amount and hatching rate of the wheat bran treatment; the residual film amount of the second generation eating farmland of each treatment is increased compared with that of the first generation, and the spawning amount of the second generation of 100% farmland residual film treatment is increased by 0.23% (P is more than 0.05) compared with that of the first generation.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (2)
1. A population continuation research method for biodegradable farmland residual films of Chilo suppressalis comprises the following steps:
(1) Cleaning and sterilizing the breeding environment: 84 disinfectant of 1:500 is used for indoor ground, 84 disinfectant of 1:100 is used for walls, windows and test tables around the incubator, and 75% alcohol solution is used for sterilizing the incubator, the insect culturing box and the tweezers;
(2) Under indoor constant temperature, constant humidity and darkroom conditions, namely 30 ℃ and 60% +/-10% RH, degrading a new film, a new PE film, a farmland residual drip irrigation tape, a farmland residual film and artificial feeding of Chilo suppressalis by using different polyethylene materials for 2 generations, wherein each generation of the process is egg-larva-pupa-adult-egg, and the specific operation is as follows: putting 80 eggs purchased in the same spawning period into a 64 cm3 hatching bottle for raising 30 d, picking larvae with the weight section of 0.1000-0.2000 g, putting into an empty insect raising box 24 h, respectively putting into an insect raising box added with 2.0000g of different polyethylene materials and 10.0000g of artificial feed and 10 heads of each box, taking out the pupa after the pupa of the larvae are formed, putting the pupa into the insect raising box in time, and putting female and male adults into the egg raising box with egg laying paper sheets in pairs after the pupa is formed, wherein the adult siphon mouth parts are degraded, so that the adult is not fed in the adult period, and taking out the egg sheets after the adult is mated and laid;
(3) Observing and recording the calendar of each stage in the whole process, and counting the weight and length changes of the larvae, the feeding capacity, the pupa weight, the pupa length, the spawning quantity, the hatching rate, the adult weight and the body length; then the spawning in the same period is fed to the next generation according to the same method; each treatment is repeated for 3 times, and the influence of different foods to the continuation of the large wax moth population is comprehensively analyzed;
(4) The measuring method comprises the following steps: ① The method for measuring the body length of the larva of the Chilo suppressalis comprises the following steps: carefully clamping 1 head of larva of Chilo suppressalis on a test table by using forceps, and measuring the length of the larva of Chilo suppressalis in sequence when the body of the larva of Chilo suppressalis linearly moves and stretches; ② The method for measuring the pupa weight and the pupa length comprises the following steps: observing twice at a timing of 10:00 and 18:00 a day, measuring pupa weight and pupa length when pupa appears, and separating to another insect-raising box; ③ Adult body weight and length measuring method: and (5) baking at 70 ℃ for 20 min after spawning is finished, and weighing the dead body weight and the body length.
2. A population continuation research method for a biodegradable farmland residual film of Chilo suppressalis is characterized by comprising the following steps:
(1) Cleaning and sterilizing the breeding environment: 84 disinfectant of 1:500 is used for indoor ground, 84 disinfectant of 1:100 is used for walls, windows and test tables around the incubator, and 75% alcohol solution is used for sterilizing the incubator, the insect culturing box and the tweezers;
(2) Under indoor constant temperature, constant humidity and darkroom conditions, namely 30 ℃ and 60% +/-10% RH, mixing wheat bran and Xinjiang farmland residual film according to different proportions, artificially feeding Chilo suppressalis for 2 generations, wherein each generation of experience stage is egg-larva-pupa-adult-egg, and the specific operation is as follows: putting 80 eggs purchased in the same spawning period into a 64 cm 3 hatching bottle, raising for 30 d, picking larvae with the weight section of 0.1000-0.2000 g, putting into an empty insect raising box 24 h, respectively putting into an insect raising box with 5.0000g wheat bran, 2.5000g wheat bran+ 2.5000g farmland plastic film residue and 5.0000g farmland plastic film residue and 10 heads of each box, taking out the insect pupae in time after pupation, putting the insect pupae into the insect raising box alone, and putting female and male adults into the egg raising boxes with egg laying paper sheets in pairs after eclosion, wherein the adult period is not fed due to degeneration of an adult siphon mouth piece, and taking out the egg laying sheets after adult mating;
(3) Observing and recording the calendar of each stage in the whole process, and counting the weight and length changes of the larvae, the feeding capacity, the pupa weight, the pupa length, the spawning quantity, the hatching rate, the adult weight and the body length; then the spawning in the same period is fed to the next generation according to the same method; each treatment is repeated for 3 times, and the influence of the ingestion of the Xinjiang farmland residual film on the continuation of the large wax moth population is comprehensively analyzed;
(4) The measuring method comprises the following steps: ① The method for measuring the body length of the larva of the Chilo suppressalis comprises the following steps: carefully clamping 1 head of larva of Chilo suppressalis on a test table by using forceps, and measuring the length of the larva of Chilo suppressalis in sequence when the body of the larva of Chilo suppressalis linearly moves and stretches; ② The method for measuring the pupa weight and the pupa length comprises the following steps: observing twice at a timing of 10:00 and 18:00 a day, measuring pupa weight and pupa length when pupa appears, and separating to another insect-raising box; ③ Adult body weight and length measuring method: and (5) baking at 70 ℃ for 20 min after spawning is finished, and weighing the dead body weight and the body length.
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