CN108244413A - A kind of control on Cryptophlebia ombrodelta Lower in litchi man-made feeds and preparation method - Google Patents
A kind of control on Cryptophlebia ombrodelta Lower in litchi man-made feeds and preparation method Download PDFInfo
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Abstract
The present invention provides a kind of control on Cryptophlebia ombrodelta Lower in litchi man-made feeds and preparation method, the feed includes mixed fodder, lures additive, coagulator and antistaling agent, and the mass percent of each ingredient is respectively in man-made feeds：Corn flour 17.58%~19.27%, dusty yeast 7.03%~7.71%, white sugar 3.52%~4.82%, sorbic acid 0.18%~0.29%, casein 1.76%~3.85%, methyl hydroxybenzoate 0.48%~0.53%, ascorbic acid 0.70%~0.77%, cholesterol 0.04%~0.06%, catsup or lichee sauce 33.72%~43.96% lure additive 3.52%~5.78%, coagulator 3.52%~3.85%, antistaling agent 0.09%~0.14%, and surplus is analysis for soybean powder.The ultra-pure water of addition mixed fodder 10~15 times of volumes of total amount is needed in process for preparation.
Description
Technical field
The invention belongs to field of feed, and in particular to a kind of control on Cryptophlebia ombrodelta Lower in litchi man-made feeds and preparation method.
Background technology
Control on Cryptophlebia ombrodelta Lower in litchi (CryptophlebiaombrodeltaL.) belongs to Lepidoptera Tortricidae abnormity steinernema category,
Alias lichee steinernema, the brown leaf roller of stain；TaiWan, China is known as thick foot Ji leaf roller, and Queensland nut producing region is known as Queensland nut
Eat into fruit snout moth's larva, Queensland nut borer.Control on Cryptophlebia ombrodelta Lower in litchi host range is wider, lichee of mainly causing harm, longan, sweet orange, Australia
The pods of the Leguminosae trees such as the fruit of the industrial crops such as nut and erythrophloeum ferdii of causing harm, asoka, Tokyo You Nan, instrument flower, seed and
Tender tip or semi-lignified tender tip.
Insect raising is of great significance for carrying out control of insect, insect physiology and ecology etc. research.It is different
Insect artificial diet's difference of type is big or even with stronger specificity.The reality of studies in China control on Cryptophlebia ombrodelta Lower in litchi at present
It tests material to capture by field acquisition, is influenced to cannot be guaranteed sample size by growth cycle, host, environmental condition etc., in addition
Carry out insect growth and behavioral study not only has high requirement to feeding environment condition, to the dependency degree higher of feed.
Incidence of the control on Cryptophlebia ombrodelta Lower in litchi in Fujian, Guangdong, Guangxi erythrophloeum ferdii Artificial Pure leads to lattice close to 100%
Wooden apical dominance is lost, it is difficult to grow into forest.Treelet insect pest incidence is also very high in its wildwood, and treelet survival is influenced when serious
And population recruitment.Therefore, it is most important that special control on Cryptophlebia ombrodelta Lower in litchi man-made feeds are researched and developed, to ensure correlative development and life
The smooth development of the researchs such as state.It investigates and finds in more ground rare tree artificial forest, control on Cryptophlebia ombrodelta Lower in litchi is only to erythrophloeum ferdii
Evil is serious, and to the non-occurring and damage of multiple seeds such as Lauraceae, Fagaceae, pulse family Dalbergia, Pterocarpus, which extremely likes erythrophloeum ferdii
It is good, and main cause harm erythrophloeum ferdii tender tip and compound leaf base portion, infer that there may be the chemicals that it is lured to lay eggs or cause harm at these positions
Matter, therefore, the man-made feeds of the control on Cryptophlebia ombrodelta Lower in litchi of focus development addition erythrophloeum ferdii branch powder or leaf powder help to find out growth
Law of development provides theory and technology support to carry out its effective prevention and control.
Invention content
In view of this, the present invention provides a kind of control on Cryptophlebia ombrodelta Lower in litchi man-made feeds and preparation method
Technical scheme is as follows：
A kind of control on Cryptophlebia ombrodelta Lower in litchi man-made feeds including mixed fodder, lure additive, coagulator and antistaling agent, institute
The mass percent for stating each ingredient of man-made feeds is respectively：Corn flour 17.58%~19.27%, dusty yeast 7.03%~
7.71%th, white sugar 3.52%~4.82%, sorbic acid 0.18%~0.29%, casein 1.76%~3.85%, Metagin
Ester 0.48%~0.53%, ascorbic acid 0.70%~0.77%, cholesterol 0.04%~0.06%, catsup or lichee sauce
33.72%~43.96%, lure additive A or lure additive B 3.52%~5.78%, coagulator 3.52%~
3.85%th, antistaling agent 0.09%~0.14%, surplus are analysis for soybean powder.Addition feed 10~15 times of volumes of total amount are needed in process for preparation
Ultra-pure water.
Further, formula I includes mixed fodder, lures additive, coagulator and antistaling agent, the man-made feeds respectively into
Point mass percent be respectively：Corn flour 17.95%, white sugar 3.99%, sorbic acid 0.25%, is done dusty yeast 7.38%
Casein 2.53%, ascorbic acid 0.76%, cholesterol 0.05%, catsup 37.91%, is lured and is added at methyl hydroxybenzoate 0.51%
Add agent A5.05%, coagulator 3.54%, antistaling agent 0.13%, surplus is analysis for soybean powder, and addition feed total amount 10 is needed in process for preparation
The ultra-pure water of~15 times of volumes.
Further, formula II includes mixed fodder, lures additive, coagulator and antistaling agent, and the man-made feeds are each
The mass percent of ingredient is respectively：Corn flour 18.95%, dusty yeast 7.58%, white sugar 3.79%, sorbic acid 0.25%,
Casein 2.53%, ascorbic acid 0.76%, cholesterol 0.05%, catsup 37.91%, is lured at methyl hydroxybenzoate 0.51%
Additive B 5.05%, coagulator 3.54%, antistaling agent 0.13%, surplus are analysis for soybean powder, and addition feed total amount is needed in process for preparation
The ultra-pure water of 10~15 times of volumes.
Further, formula III includes mixed fodder, lures additive, coagulator, antistaling agent and ultra-pure water, described artificial
The mass percent of each ingredient of feed is respectively：Corn flour 18.45%, dusty yeast 7.58%, white sugar 3.79%, sorbic acid
0.25%th, casein 2.53%, methyl hydroxybenzoate 0.51%, ascorbic acid 0.76%, cholesterol 0.05%, lichee sauce
38.61%th, additive A 5.05%, coagulator 3.54%, antistaling agent 0.13% are lured, surplus is analysis for soybean powder, is needed in process for preparation
Add the ultra-pure water of feed 10~15 times of volumes of total amount.
Further, formula IV includes mixed fodder, lures additive, coagulator and antistaling agent, and the man-made feeds are each
The mass percent of ingredient is respectively：Corn flour 17.95%, dusty yeast 7.58%, white sugar 3.79%, sorbic acid 0.25%,
Casein 2.53%, ascorbic acid 0.76%, cholesterol 0.05%, lichee sauce 37.91%, is lured at methyl hydroxybenzoate 0.51%
Additive B 5.05%, coagulator 3.54%, antistaling agent 0.13%, surplus are analysis for soybean powder, and addition feed total amount is needed in process for preparation
The ultra-pure water of 10~15 times of volumes.
Further, formula V includes mixed fodder, coagulator and antistaling agent, the quality hundred of each ingredient of man-made feeds
Point ratio is respectively：Corn flour 25.80%, dusty yeast 10.32%, white sugar 5.16%, sorbic acid 0.34%, methyl hydroxybenzoate
0.69%th, ascorbic acid 1.03%, cholesterol 0.07%, catsup 51.60%, coagulator 4.82%, antistaling agent 0.17%, it is remaining
It measures as analysis for soybean powder, the ultra-pure water of addition feed 10~15 times of volumes of total amount is needed in process for preparation.
Further, it is described to lure additive A and to lure additive B be respectively erythrophloeum ferdii leaf powder and erythrophloeum ferdii branch powder, coagulator
It is formaldehyde for agar, antistaling agent.
The present invention also provides a kind of preparation methods of control on Cryptophlebia ombrodelta Lower in litchi man-made feeds, include the following steps：
Step 1) is stocked up, and is obtained mixed fodder, is lured additive, coagulator, antistaling agent and ultra-pure water spare；
Step 2) adds in 1/ after weighing corn flour, analysis for soybean powder, dusty yeast, erythrophloeum ferdii branch powder or erythrophloeum ferdii leaf powder mixing in proportion
The ultra-pure water stirring of 3 total amounts is for use, and ferment 5~8h；
Step 3) takes agar powder in proportion, adds in the ultra-pure water of 2/3 total amount, boils and be allowed to dissolve fully, cools down about 3min,
Then sorbic acid, methyl hydroxybenzoate, ascorbic acid, cholesterol, formaldehyde, white sugar are proportionally added into；
Step 4) adds in corn flour, analysis for soybean powder, dusty yeast, erythrophloeum ferdii leaf powder or erythrophloeum ferdii branch powder that step 2) fermentation is completed
Mixture simultaneously stirs evenly, and a small amount of ultra-pure water can be added to be tuned into paste, catsup or lichee sauce is eventually adding, after stirring evenly rapidly
It pours into plastic casing, it is for use that refrigeration in 4 DEG C of refrigerators is put into after its cooling.
The beneficial effects of the invention are as follows：
Artificial feeding is carried out to control on Cryptophlebia ombrodelta Lower in litchi by the feed of offer, the hair of control on Cryptophlebia ombrodelta Lower in litchi can be promoted
It educates, improves artificial feeding effect, compared with not adding the formula of attractant, the larva of optimization formula goes through the phase and pupa goes through phase shortening
8%~9%, larva survival rate improves 23%, and pupa weight and percentage of pupation improve 19% and 12%, and cumulative survival rate improves 48%, production
Ovum amount improves 16%, helps to probe into the growth and development situation of control on Cryptophlebia ombrodelta Lower in litchi and Biological Mechanism.
Description of the drawings
Fig. 1 compares figure for influence of 5 kinds of feed formulas to control on Cryptophlebia ombrodelta Lower in litchi growth and development in the present invention.
Specific embodiment
Technical scheme of the present invention is further explained below by embodiment, but protection scope of the present invention is not by reality
Apply the limitation of example in any form.
A kind of formula of control on Cryptophlebia ombrodelta Lower in litchi man-made feeds of the present invention and preparation method thereof, includes the following steps：
Step 1) is stocked up, and is obtained mixed fodder, is lured additive, coagulator, antistaling agent and ultra-pure water spare；
After step 2) weighs corn flour, analysis for soybean powder, dusty yeast, erythrophloeum ferdii branch powder or erythrophloeum ferdii leaf powder mixing in proportion, add in
The ultra-pure water of 1/3 total amount stirs evenly, and ferment 5~8h；
Step 3) takes agar powder in proportion, adds in the ultra-pure water of 2/3 total amount, boils and be allowed to dissolve fully, cools down about 3min,
Then sorbic acid, methyl hydroxybenzoate, ascorbic acid, cholesterol, formaldehyde, white sugar are proportionally added into；
Step 4) adds in corn flour, analysis for soybean powder, dusty yeast, erythrophloeum ferdii leaf powder or erythrophloeum ferdii branch powder that step 2) fermentation is completed
Mixture simultaneously stirs evenly, and a small amount of ultra-pure water can be added to be tuned into paste, is eventually adding catsup or lichee sauce, stirs evenly rapidly
After pour into plastic casing, it is for use that refrigeration in 4 DEG C of refrigerators is put into after its cooling.
It is the Application Example of feed prepared by this method below.
Selected insect source and its primary culture
In acquiring tool yellow-white unqualified shape substance on branch in erythrophloeum ferdii artificial forest, (control on Cryptophlebia ombrodelta Lower in litchi is caused harm initial stage disease
Shape) erythrophloeum ferdii branch and take back laboratory, be first placed in moisturizing raising box, be subsequently placed in growth cabinet and cultivate, culture
Condition is that temperature is 25 DEG C, relative humidity 80%, and the photoperiod is illumination 15h, dark 9h.It is different by lichee using erythrophloeum ferdii branch
Shape steinernema completes larvae development, treats that larvae pupation is placed in round culture box, until pupa turns into adult.Lichee abnormity is small
Roll up the raising of moth
(1) collection and hatching of ovum：After adult eclosion, each both ends of female male imago of same day emergence are chosen, are put into simultaneously
In 1500ml insect box, template is placed in bottom, collects a deutovum paper every other day, and ovum and ovum paper are put into culture dish together,
Go out larva to egg hatching, culture dish is put into the cotton balls moisturizing for being moistened with 5% hydromel.
(2) larva raising：Raising culture dish accesses the larva after just hatching, and both ends children is put into each culture dish
Worm, and prepared feed is put into culture dish, feed is replaced according to raising situation in time.Larva appetite after 5 ages is acute
Increase, replacement feed needs are more frequent, in addition the sundries and excrement in the culture dish of timing cleaning daily.
(3) it pupates and sprouts wings：The survival condition of larva is observed in timing daily, in larva last period in age, records linal-instar larvae
Transformation situation, and in time record pupate the time；The emergence situation of pupa is observed in timing daily, until pupa turns into adult, records it
Emergence situation.
(4) it mates and lays eggs：Each both ends of female male imago of same day emergence are chosen, adult is moved into 1500ml insect box,
Bottom is placed the template one made and is opened for its oviposition, by the use of the cotton balls for being moistened with 5% hydromel as extra-nutrition source, remembers daily
Record egg laying amount.
Instance analysis
With reference to Fig. 1, Fig. 1 compares for influence of 5 kinds of feed formulas to control on Cryptophlebia ombrodelta Lower in litchi growth and development.
5 kinds of feed formulas are gone through phase and Life of Adult to the larvae development of control on Cryptophlebia ombrodelta Lower in litchi and are had a significant impact, to pupa
It is not notable to go through phase influence.From the point of view of entire development duration, the effect of feed formula II is best, the larva of control on Cryptophlebia ombrodelta Lower in litchi and
The development duration of pupa is most short, respectively 19.15d and 8.10d, and relatively formula V declines 8%~9% respectively；Life of Adult is matched
Square II longest is 9.28d, and relatively formula V improves 15%, i.e., formula II can both shorten larva and pupa goes through the phase, while improve adult
Service life.Survival rate of larvae and percentage of pupation significant difference between formula, wherein it is optimal with formula II, respectively 82.4% and 90.2%,
23% and 12% is improved compared with formula V, formula V takes second place.Different feeds are to the weight and eclosion rate of control on Cryptophlebia ombrodelta Lower in litchi pupa
Significant influence is influencing not notable, pupa weight, the survival rate of pupa and the eclosion rate of feed formula II to the survival rate of pupa
Higher than other 4 kinds of feed formulas, respectively 48.57g, 80.2% and 91.8%, relatively formula V improve 19%, 3% and 4%, match
Side V takes second place.Influence of the different feeds to the egg laying amount of control on Cryptophlebia ombrodelta Lower in litchi adult is notable, wherein the egg laying amount of formula II is high
It it is 53.3 in other formulas, relatively formula V improves 16%.
Survival rate be judge raising whether one of a successful important index, it is that evaluation insect is that can offspring, which breed,
A no important biological indicator for being capable of mass rearing, the pupa of offspring are again bigger, it was demonstrated that the fecundity of female adult is higher.It is comprehensive
From the point of view of closing, the otherness of feed formula is mainly manifested in larval stage, and the influence to pupa is smaller, but to adult eclosion and oviposition shadow
It rings notable.Cumulative survival rate (larva survival rate × percentage of pupation × pupa survival rate × eclosion rate) is ordered as formula II (55%)>Match
IV (46%) of side>It is formulated I (39%)>It is formulated V (37%)>III (26%) is formulated, the sequences such as pupa weight, eclosion rate, egg laying amount are therewith
Unanimously.
4 kinds of powder of branch containing erythrophloeum ferdii of this research or the feed formula raising control on Cryptophlebia ombrodelta Lower in litchi of leaf powder can complete the population
Generation development, in addition to formula III, the feed formula of the powder of branch containing erythrophloeum ferdii or leaf powder (formula II, formula IV, formula I) is superior to not
The feed (formula V) of erythrophloeum ferdii ingredient is added, adds the formula (formula II, formula IV) of erythrophloeum ferdii branch powder better than addition erythrophloeum ferdii leaf powder
Formula (formula I, formula III), add formula (formula I, formula II, formula V) matching better than addition lichee sauce of catsup
Side's (formula III, formula IV).It is small that II pair of formula of the comprehensive analysis based on adding erythrophloeum ferdii branch powder, catsup shortens lichee abnormity
Moth development duration is rolled up, survival rate is improved, increases pupa weight, egg laying amount etc. the most significantly.
Better embodiment for the present invention above, but embodiment is not for limiting the present invention, is not departing from this
Any equivalence changes done in the spirit and scope of invention or retouching also belong to the protection domain of the present invention, therefore this hair
Bright protection domain should be using the content that claims hereof is defined as standard.
Claims (8)
- A kind of 1. control on Cryptophlebia ombrodelta Lower in litchi man-made feeds, which is characterized in that the mass percent of each ingredient point in the man-made feeds It is not：Corn flour 17.58%~19.27%, dusty yeast 7.03%~7.71%, white sugar 3.52%~4.82%, sorbic acid 0.18%~0.29%, casein 1.76%~3.85%, methyl hydroxybenzoate 0.48%~0.53%, ascorbic acid 0.70%~ 0.77%th, cholesterol 0.04%~0.06%, catsup or lichee sauce 33.72%~43.96%, lure additive A or lure Additive B 3.52%~5.78%, coagulator 3.52%~3.85%, antistaling agent 0.09%~0.14%, surplus are analysis for soybean powder, The ultra-pure water of addition feed 10~15 times of volumes of total amount is needed in process for preparation.
- 2. control on Cryptophlebia ombrodelta Lower in litchi man-made feeds according to claim 1, which is characterized in that each ingredient in the man-made feeds Mass percent be respectively：Corn flour 17.95%, dusty yeast 7.38%, white sugar 3.99%, sorbic acid 0.25%, cheese Element 2.53%, ascorbic acid 0.76%, cholesterol 0.05%, catsup 37.91%, lures addition at methyl hydroxybenzoate 0.51% Agent A5.05%, coagulator 3.54%, antistaling agent 0.13%, surplus are analysis for soybean powder, needed in process for preparation addition feed total amount 10~ The ultra-pure water of 15 times of volumes.
- 3. control on Cryptophlebia ombrodelta Lower in litchi man-made feeds according to claim 1, which is characterized in that each ingredient in the man-made feeds Mass percent be respectively：Corn flour 18.95%, dusty yeast 7.58%, white sugar 3.79%, sorbic acid 0.25%, cheese Element 2.53%, ascorbic acid 0.76%, cholesterol 0.05%, catsup 37.91%, lures addition at methyl hydroxybenzoate 0.51% Agent B5.05%, coagulator 3.54%, antistaling agent 0.13%, surplus are analysis for soybean powder, needed in process for preparation addition feed total amount 10~ The ultra-pure water of 15 times of volumes.
- 4. control on Cryptophlebia ombrodelta Lower in litchi man-made feeds according to claim 1, which is characterized in that each ingredient in the man-made feeds Mass percent be respectively：Corn flour 18.45%, dusty yeast 7.58%, white sugar 3.79%, sorbic acid 0.25%, cheese Element 2.53%, ascorbic acid 0.76%, cholesterol 0.05%, lichee sauce 38.61%, lures addition at methyl hydroxybenzoate 0.51% Agent A5.05%, coagulator 3.54%, antistaling agent 0.13%, surplus are analysis for soybean powder, needed in process for preparation addition feed total amount 10~ The ultra-pure water of 15 times of volumes.
- 5. control on Cryptophlebia ombrodelta Lower in litchi man-made feeds according to claim 1, which is characterized in that each ingredient in the man-made feeds Mass percent be respectively：Corn flour 17.95%, dusty yeast 7.58%, white sugar 3.79%, sorbic acid 0.25%, cheese Element 2.53%, ascorbic acid 0.76%, cholesterol 0.05%, lichee sauce 37.91%, lures addition at methyl hydroxybenzoate 0.51% Agent B5.05%, coagulator 3.54%, antistaling agent 0.13%, surplus are analysis for soybean powder, needed in process for preparation addition feed total amount 10~ The ultra-pure water of 15 times of volumes.
- 6. the control on Cryptophlebia ombrodelta Lower in litchi man-made feeds according to claims 1 or 2 or 4, it is characterised in that described to lure addition Agent A is erythrophloeum ferdii branch powder, and coagulator is agar, and antistaling agent is formaldehyde.
- 7. according to the control on Cryptophlebia ombrodelta Lower in litchi man-made feeds described in claim 1 or 3 or 5, it is characterised in that described to lure addition Agent B is erythrophloeum ferdii leaf powder, and coagulator is agar, and antistaling agent is formaldehyde.
- 8. a kind of preparation method of control on Cryptophlebia ombrodelta Lower in litchi man-made feeds, which is characterized in that include the following steps：Step 1) is stocked up, and is obtained mixed fodder, is lured additive, coagulator, antistaling agent and ultra-pure water spare；Step 2) weighs corn flour, analysis for soybean powder, dusty yeast, erythrophloeum ferdii branch powder or leaf powder in proportion, and 1/3 total amount is added in after mixing Ultra-pure water stirs evenly, and ferments 5~8 hours；Step 3) takes agar powder in proportion, adds in the ultra-pure water of 2/3 total amount, boils and be allowed to dissolve fully, after cooling down about 3min, presses Ratio adds in sorbic acid, methyl hydroxybenzoate, ascorbic acid, cholesterol, formaldehyde, white sugar；Step 4) adds in the mixing of corn flour, analysis for soybean powder, dusty yeast, erythrophloeum ferdii leaf powder or erythrophloeum ferdii branch powder that step 2) fermentation is completed Object simultaneously stirs evenly, and a small amount of ultra-pure water can be added to be tuned into paste, is finally proportionally added into catsup or lichee sauce, stirs evenly rapidly After pour into plastic casing, treat that its cooling prepares completion, storage can be placed in 4 DEG C of refrigerator cold-storages.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713496A (en) * | 2019-03-22 | 2020-09-29 | 广西壮族自治区林业科学研究院 | Efficient sex attractant for litchi special-shaped cabbage moths |
| CN114304073A (en) * | 2022-01-12 | 2022-04-12 | 广东省农业科学院植物保护研究所 | Method for artificial feeding and worm egg collection of neovaricella deltoidea |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101731482A (en) * | 2010-02-08 | 2010-06-16 | 西北农林科技大学 | Artificial feed for Grapholitha molesta Busck larvae and preparation method thereof |
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| CN111713496A (en) * | 2019-03-22 | 2020-09-29 | 广西壮族自治区林业科学研究院 | Efficient sex attractant for litchi special-shaped cabbage moths |
| CN114304073A (en) * | 2022-01-12 | 2022-04-12 | 广东省农业科学院植物保护研究所 | Method for artificial feeding and worm egg collection of neovaricella deltoidea |
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