CN100360049C - Method for producing maggot protein by using ecological treatment of after shelf-like food - Google Patents
Method for producing maggot protein by using ecological treatment of after shelf-like food Download PDFInfo
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- CN100360049C CN100360049C CNB2005100158798A CN200510015879A CN100360049C CN 100360049 C CN100360049 C CN 100360049C CN B2005100158798 A CNB2005100158798 A CN B2005100158798A CN 200510015879 A CN200510015879 A CN 200510015879A CN 100360049 C CN100360049 C CN 100360049C
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Abstract
The present invention relates to a method for preparing fly maggot protein from expired food by ecological treatment. By using house fly larvae which serve as a bioreactor for converting waste protein in expired food to fly maggot protein which can be used for providing food processing of feed. The aim of decrement and innocent treatment of food and resource utilization are achieved by procedures such as expired food treatment, fly cultivation, fly-blow collection, fly-blow inoculation, fly maggot protein separation by fly maggots, etc. The content of obtained larvae contains 58 to 62 percent of protein, the conversion rate respectively comprises 24 to 19 percent of milk products, 28 to 30 percent of meat products, 27 to 30 percent of water products and 23 to 32 percent of cereal wastes. The present invention improves biological efficacy of food chains, and besides, the present invention has favorable social, economic and environmental benefits.
Description
Technical field
The present invention relates to a kind of method of producing maggot protein by using ecological treatment of after shelf-like food.
Background technology
Expired food is meant the food that surpasses safe-use period or shelf-life, be that State Administration for Industry and Commerce's explicit order defective food of releasing market that should stop immediately marketing is the food security of guaranteeing the consumer, expired food should be removed frame and recall concentrated the destruction, main destroying method is to bury or burn this disposal processing method can bring certain influence to environment, and uneconomical, do not meet the principle of recycling.
Summary of the invention
The object of the invention provides a kind of method of producing maggot protein by using ecological treatment of after shelf-like food, be mostly to be organic waste at expired food, can become the characteristics of utility through bio-transformation, utilizing common house-fly grub as bioreactor discarded protein in the expired food to be transformed into can provide fly-maggot protein that the feed food processing uses with the biopotency that the purpose the present invention who reaches expired food minimizing harmless treatment and recycling has improved food chain, has good socialbenefit and economic effect and environmental benefit.
The method of producing maggot protein by using ecological treatment of after shelf-like food of the present invention, comprise that kind of an expired food processing fly cultivation fly blow production, fly blow are collected the fly blow inoculation, fly maggot separates operations such as fly-maggot protein processing, make fly-maggot protein product obsolescence food and generally concentrate on supermarket and food processing factory, mainly contain dairy produce, meat product, cereal product, aquatic products etc.With the expired food concentrated by above classification unpack drying, pulverize stand-by.
The kind fly of adopting is housefly (Musca domestica).Be by luring the hatching of the collection ovum that wild housefly produced at first.
With the common house-fly grub is that bioreactor transforms expired food: the fly blow of centralized collection is except that small part keeps kind of fly, and major part is as the bio-transformation of food waste.The food waste of having handled is added cereal discarded object wheat bran preparation hatching material by a certain percentage, the ovum material that connects that contains ovum is layered on the hatching material.The hatching material that connects ovum is put into incubator, be placed in 28 ℃ the culturing room.Food waste for the hatching of larva provides a large amount of mycelium 3-5 days, promptly hatches larva after abundant fermentation.Before larvae pupation, utilize common house-fly grub to have negative phototactic characteristics, carry out the worm material with manual method and separate, the larva that is separated to is exactly fly-maggot protein after treatment.
The method of producing maggot protein by using ecological treatment of after shelf-like food of the present invention comprises following concrete steps in detail:
1) will collect expired food and unpack, classify, pulverize, mix, dehydrate the place.Wheat bran is used as major ingredient and other expired food of common house-fly grub hatching material, is prepared into the hatching material; The part by weight of wheat bran and expired food is respectively:
Wheat bran: milk powder=1: 0.1～0.3
Wheat bran: liquid milk=1: 0.3～0.5
Wheat bran: meat products=1: 0.27～0.4
Wheat bran: aquatic products=1: 0.34～0.5.
2) cleaning of planting fly is cultured:
(1) under field conditions (factors), the saprophytic food that utilizes housefly happiness food lures the wild housefly of collection (Musca domestica) hatching kind of the fly that lays eggs with fly trapping cage, and totally-enclosed artificial feeding kind fly use screen cloth is made the fly cage; With plastic box as the larva raising box, box sizes: 60cm * 35cm * 15cm.
(2) raising of one-tenth fly: pupa is placed in the fly cage, and 25 ℃～32 ℃ conditions are cultivated and were become fly to sprout wings out in 3-4 days, put into feed and water, and becoming the fly feed is that milk powder and white sugar were prepared by 1: 1, and every day, feed was changed once, water replacing secondary; Become fly emergence to begin mating after 2-3 days, begin to lay eggs after 3-4 days, the egg-laying season can keep about 15 days.
(3) collection of fly blow: when becoming fly to sprout wings by 3 days, put in the cage and connect the ovum box, connecing the ovum material is to be soaked by wheat bran to form, and humidity is 60-70%; Worm's ovum centralized collection every day twice guarantees that collected ovum larva hatching has synchronism.
(4) hatching of larva: the hatching material is that major ingredient adds the treated expired food of step 1) in proportion with wheat bran, the hatching material contains plastic box, adding water stirs, humidity remains on the ovum material that connects that 50-70% will contain worm material and sneaks into equably in the hatching material, raises the density of 15000 larvas by the per kilogram material and raises.Expect thick 10-12cm.28-30 ℃ of hatching indoor temperature, temperature 40-50 ℃ is the suitableeest incubation condition in the feed.
(5) collection of larva and pupating: the larva of visible hatching after 24 hours, can obtain the larva of 4-5 order after 3-5 days, utilize the negative phototaxis of common house-fly grub, larva can be bored toward the feed depths automatically, and feed floats automatically, constantly removes the feed on upper strata, just can isolate larva.Obtain larva after the worm material separates, pupated after under the condition of drying 2 days, the pupa of selecting high-quality stays the emergence of doing kind of fly.
3) expired food and wheat bran are prepared into the hatching material in proportion behind the mixing and are contained in the plastic box; The worm's ovum that step (3) and (4) obtain after kind of fly is done in the reservation of small part worm's ovum, is sneaked into plastic box with most of worm's ovum and is transformed expired food as bioreactor; The worm material can separate under illumination condition, utilizes common house-fly grub to bear phototaxis, and larva will lucifuge down be bored, and the worm material is separated, and removes the hatching material on upper strata, or with 16～20 purpose stainless steel mesh fly maggot is sifted out, and promptly obtains a large amount of larvas;
4) fly maggot larva live body is put to death through hot water earlier, under 60 ℃ of conditions, dry again fly-maggot protein.
The present invention utilizes common house-fly grub to transform food waste as bioreactor, and the changing effect of discarded albumen is obvious, and gained larvae protein content is 58%～62%, and conversion ratio is respectively:
Dairy produce: 24%～29%
Meat products: 28%～30%
Aquatic products: 27%～30%
Cereal discarded object: 23%～32%.
The present invention utilizes common house-fly grub as bioreactor the fly-maggot protein that can provide the feed food processing to use to be provided discarded protein in the expired food.Reached the purpose of expired food minimizing, harmless treatment and recycling.The present invention has improved the biopotency of food chain, has good social benefit, economic benefit and environmental benefit.
Description of drawings
Expired milk powder of Fig. 1 and wheat bran.
Fig. 2 is hatched material.
Hatching material in Fig. 3 fermentation.
Fig. 4 adult housefly.
Fig. 5 common house-fly grub.
Fig. 6 housefly pupa.
The specific embodiment
Embodiment
The present invention can describe in detail by embodiment, but they are not that the present invention is done any restriction.
Collect expired food:
Buy expired food dairy produce (milk powder and liquid milk), meat product (cooked meat product), aquatic products, wheat bran from the supermarket and the market of farm produce.Wheat bran is the cereal discarded object of grain processing plant, and protein content is higher in expired food and the discarded object, measures through Kjeldahl and sees Table 1:
Table 1: discarded Protein content in the expired food
| The hatching material | The dry total protein content |
| Wheat bran | 18.54％ |
| Milk powder | 19.90％ |
| Milk | 2.9g/100ml |
| Ham sausage | 30.59％ |
| The angle | 60.00％ |
The expired food of commodity packaging is unpacked earlier, classifies, pulverizes, is mixed, and dry back is stand-by.Water content higher preferably with collecting with utilization, unavailablely at that time should dehydrate preservation.Wheat bran is used as major ingredient and other expired food of common house-fly grub hatching material.Ratio is respectively:
Wheat bran: milk powder=1: 0.1～0.3
Wheat bran: liquid milk=1: 0.3～0.5
Wheat bran: meat products=1: 0.27～0.4
Wheat bran: aquatic products=1: 0.34～0.5.
Adopt the totally-enclosed artificial feeding kind of clean cultural technique fly:
The first, the collection epidermis is vivid, stature is moderate, normotrophic pupa, pupa is put into the clorox of disinfectant 2% and is sterilized 30 minutes, after the surface sterilization sterilization, puts kind of a fly cage into and waits to sprout wings;
Second, become the raising of fly to adopt cage system, sprouting wings in the washing back, sprouts wings and the raising condition: 25 ℃-32 ℃ of temperature, the nature of every day or artificial lighting 5 hours, with this understanding, can sprout wings into fly in 3-4 days, and become the raising of fly to use man-made feeds and clean water, after each breeding cycle is finished, wash all utensils, raising instrument and receptacle are carried out the routine sterilization;
The 3rd, the collection of fly blow: be placed with in the cage and connect the ovum dish, the one-tenth fly of mating begins to lay eggs after three days, and every day, centralized collection was twice;
The 4th, larva raising: select the ovum of high-quality for use, hatch larva raising separately, feeds utilized is wheat bran.Through pupating, sprouting wings, repeat above process, keep kind of a fly.
Planting the cleaning of fly cultures:
Kind of fly is to lure the wild housefly of collection (Musca domestica) hatching of laying eggs under the natural conditions.The fly cage is to make 80cm * 80cm * 80cm by screen cloth; The larva raising box is the plastic box of market sale, box sizes: 60cm * 35cm * 15cm.
Become the raising of fly: pupa is placed in the fly cage, and 25 ℃～32 ℃ conditions cultivations became fly to sprout wings out in 3-4 days, put into feed and water, and becoming the fly feed is that milk powder and white sugar were prepared by 1: 1, and water is clean running water, and every day, feed was changed water replacing secondary one time.Become fly emergence to begin mating after 2-3 days, begin to lay eggs after 3-4 days, the egg-laying season can keep about 15 days.
The collection of fly blow: when becoming fly to sprout wings by 3 days, put in the cage and connect the ovum box, connecing the ovum material is to be soaked by wheat bran to form, and humidity is 60-70%.Worm's ovum centralized collection every day twice guarantees that collected ovum larva hatching has synchronism.
The hatching of larva and the conversion of expired food: the hatching material is a major ingredient with wheat bran, by 1: the 0.1 treated expired milk powder (nest's milk powder) of interpolation, the hatching material contains plastic box, adding water stirs, humidity remains on the ovum material that connects that 50-70% will contain worm material and sneaks into equably in the hatching material, raises the density of 15000 larvas by the per kilogram material and raises.Expect thick 12cm.28-30 ℃ of hatching indoor temperature, temperature 40-50 ℃ is the suitableeest incubation condition in the feed.
The collection box of larva is pupated: the larva of visible hatching after 24 hours, can obtain the larva that 4-5 makes after 3-5 days, and utilize the negative phototaxis of common house-fly grub, larva can be bored toward the feed depths automatically, and feed floats automatically, constantly removes the feed on upper strata, just can isolate larva.Separate through the worm material, obtain larva, pupated after under the condition of drying 2 days, the pupa of selecting high-quality stays the emergence of doing kind of fly.
The conversion of expired food:
The expired milk powder that processing is collected (nest's milk powder) was prepared into the hatching material to wheat bran and nest's milk powder in 1: 0.1 and is contained in the plastic box behind the ratio mixing, contain 7.5 kilograms in feed in each Turnover Box, and wherein wheat bran is 6.82 kilograms, 0.68 kilogram of expired milk powder.Expect thickly, connect several about 90,000 of ovum (can adjust stocking density) according to required larva polypide size at 10-12cm.
As stated above, remove the small part worm's ovum keep do kind of fly after, most of worm's ovum is sneaked into plastic box, under the optimal conditions of knowing, raise, transform expired food as bioreactor.A large amount of worm material separate and can utilize common house-fly grub to bear phototaxis under illumination condition, and larva will lucifuge down be bored, and the worm material is separated, and remove the hatching material on upper strata, promptly obtain 1.8 kilograms of bright larvas.Fly maggot larva live body is put to death through hot water earlier, under 60 ℃ of conditions, dry again 0.35 kilogram of fly maggot dry.
The changing effect of expired milk powder: gained larvae protein content is 58%～62%, albumen conversion ratio 24%～29%.
Annotate: computing formula is
Use above-mentioned same experiment condition changing effect: conversion ratio is respectively:
Dairy produce: 24%～29%
Meat products: 28%～30%
Aquatic products: 27%～30%
Cereal discarded object: 23%～32%.
Claims (3)
1, a kind of method of producing maggot protein by using ecological treatment of after shelf-like food comprises the preparation of fly larva hatching material, and the kind fly is cultured, the kind maggot is raised and the processing of fly maggot larva, it is characterized in that the following step of described method process:
1) the expired food that will collect is unpacked, classifies, pulverizes, is mixed, and dehydrates processing; The wheat bran major ingredient that hatching is expected as common house-fly grub is become the hatching material with expired food preparation; Described expired food is milk powder, dairy produce, meat products or aquatic products; The part by weight of wheat bran and expired food is respectively:
Wheat bran: milk powder=1: 0.1～0.3
Wheat bran: liquid milk=1: 0.3～0.5
Wheat bran: meat products=1: 0.27～0.4
Wheat bran: aquatic products=1: 0.34～0.5;
2) cleaning of planting fly is cultured:
(1) under field conditions (factors), utilize the saprophytic food of housefly happiness food to be bait, lure the wild housefly of collection (Muscadomestica) to lay eggs with fly trapping cage, lay eggs obtains kind of a fly through hatching; The fly cage that totally-enclosed artificial feeding kind fly uses screen cloth to make; With plastic box as the larva raising box;
(2) raising of one-tenth fly: pupa is placed in the fly cage, and 25 ℃～32 ℃ conditions are cultivated and were become fly to sprout wings out in 3-4 days, put into feed and water, and becoming the fly feed is that expired milk powder and white sugar were prepared by 1: 1, and every day, feed was changed once, water replacing secondary; Become fly emergence to begin mating after 2-3 days, begin to lay eggs after 3-4 days, the egg-laying season can keep about 15 days;
(3) collection of fly blow: when becoming fly to sprout wings by 3 days, put in the cage and connect the ovum box, connecing the ovum material is to be soaked by wheat bran to form, and humidity is 60-70%; Worm's ovum centralized collection every day twice guarantees that collected ovum has synchronism when larva hatches;
(4) hatching of larva: hatching material is a major ingredient with wheat bran, set by step 1) ratio is added treated expired food, and the hatching material contains plastic box, adds water and stirs, and humidity remains on 50-70%; The ovum material that connects that will contain the worm material is sneaked in the hatching material equably, raises the density of 15000 larvas by the per kilogram material and raises; 28-30 ℃ of hatching indoor temperature, interior temperature 40-50 ℃ of feed;
(5) collection of larva and pupating: the larva of visible hatching after 24 hours, can obtain the 4-5 larva in age after 3-5 days, larva can be bored toward the feed depths automatically, and feed floats automatically, constantly removes the feed on upper strata, isolates larva; Obtain larva after the worm material separates, pupated after under the condition of drying 2 days, the pupa of selecting high-quality stays the emergence of doing kind of fly;
3) expired food and wheat bran set by step 1) be prepared into the hatching material behind the ratio mixing and be contained in the plastic box; The worm's ovum that step (3) and (4) obtain after kind of fly is done in the reservation of small part worm's ovum, is sneaked into plastic box with most of worm's ovum and is transformed expired food as bioreactor; Worm, material separate under illumination condition, and the larva lucifuge is down bored, and the worm material is separated, and remove the hatching material on upper strata, or with 16～20 purpose stainless steel mesh fly maggot are sifted out, and promptly obtain a large amount of larvas;
4) fly maggot larva live body is put to death through hot water earlier, under 60 ℃ of conditions, dry again fly-maggot protein.
2,, it is characterized in that described fly cage is of a size of 80cm * 80cm * 80cm according to the method for the described producing maggot protein by using ecological treatment of after shelf-like food of claim 1; Described plastic box box sizes is 60cm * 35cm * 15cm.
3, according to the method for the described producing maggot protein by using ecological treatment of after shelf-like food of claim 1, it is characterized in that step 2) cleaning of the planting fly hatching material thickness of culturing described larva hatching is 10-12cm.
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| FR2930116B1 (en) * | 2008-04-16 | 2017-02-03 | Inst De Rech Pour Le Dev | PRODUCTION AND USE OF LIVING MINI-LARVAES OF INSECTS FOR AQUARIUM FISH FEEDING AND / OR ALEVINS OF ANIMAL FISH |
| CN104874584B (en) * | 2015-05-12 | 2017-04-26 | 浙江大学 | Feed preparation system and method suitable for a worm biological reactor |
| CN108770789A (en) * | 2018-02-11 | 2018-11-09 | 宁波七禾加百列农业科技有限公司 | Fly pieces of an egg hatch material and its hatching method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1328766A (en) * | 2000-06-16 | 2002-01-02 | 江来章 | Production technique of fast-production regeneration animal protein 'fly larva protein' |
| CN1439285A (en) * | 2002-06-13 | 2003-09-03 | 蒋甫定 | Fly maggot powder and producing process thereof |
| CN1579196A (en) * | 2004-05-18 | 2005-02-16 | 李发明 | Engineered fly biological high-protein powder |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1328766A (en) * | 2000-06-16 | 2002-01-02 | 江来章 | Production technique of fast-production regeneration animal protein 'fly larva protein' |
| CN1439285A (en) * | 2002-06-13 | 2003-09-03 | 蒋甫定 | Fly maggot powder and producing process thereof |
| CN1579196A (en) * | 2004-05-18 | 2005-02-16 | 李发明 | Engineered fly biological high-protein powder |
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