CN104334031A - Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same - Google Patents
Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same Download PDFInfo
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- CN104334031A CN104334031A CN201380027010.8A CN201380027010A CN104334031A CN 104334031 A CN104334031 A CN 104334031A CN 201380027010 A CN201380027010 A CN 201380027010A CN 104334031 A CN104334031 A CN 104334031A
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- VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 description 1
- 229960002894 oxiconazole nitrate Drugs 0.000 description 1
- WVNOAGNOIPTWPT-NDUABGMUSA-N oxiconazole nitrate Chemical compound O[N+]([O-])=O.ClC1=CC(Cl)=CC=C1CO\N=C(C=1C(=CC(Cl)=CC=1)Cl)/CN1C=NC=C1 WVNOAGNOIPTWPT-NDUABGMUSA-N 0.000 description 1
- 150000004291 polyenes Chemical class 0.000 description 1
- 229960001589 posaconazole Drugs 0.000 description 1
- RAGOYPUPXAKGKH-XAKZXMRKSA-N posaconazole Chemical compound O=C1N([C@H]([C@H](C)O)CC)N=CN1C1=CC=C(N2CCN(CC2)C=2C=CC(OC[C@H]3C[C@@](CN4N=CN=C4)(OC3)C=3C(=CC(F)=CC=3)F)=CC=2)C=C1 RAGOYPUPXAKGKH-XAKZXMRKSA-N 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229960002607 sulconazole Drugs 0.000 description 1
- 239000012622 synthetic inhibitor Substances 0.000 description 1
- 229960000580 terconazole Drugs 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960004214 tioconazole Drugs 0.000 description 1
- 229940075466 undecylenate Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229960004740 voriconazole Drugs 0.000 description 1
- BCEHBSKCWLPMDN-MGPLVRAMSA-N voriconazole Chemical compound C1([C@H](C)[C@](O)(CN2N=CN=C2)C=2C(=CC(F)=CC=2)F)=NC=NC=C1F BCEHBSKCWLPMDN-MGPLVRAMSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/32—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from hydrolysates of wood or straw
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Inorganic Chemistry (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to artificial feed for the mass culture of long-horned beetles, to a method for manufacturing the artificial feed, and to a method for breeding long-horned beetles using same. The artificial feed for long-horned beetles according to the present invention contains oak powder irradiated with microwaves, and thus improves the initial development of larvae, decreases the larval age gap between hatched larvae and full-grown larvae, and improves the larval survival rate by increasing resistance to pathogenic bacteria and the like. In other words, according to the present invention, long-horned beetles which normally cannot be artificially bred can be bred year-round, and long-horned beetles can be bred in large quantities by increasing the larval survival rate and emergence rate.
Description
Technical field
The present invention relates to a kind of for the man-made feeds of the huge longicorn of amount reproduction, the manufacture method of man-made feeds and utilize these man-made feeds to raise the method for huge longicorn.
Background technology
At present, breed and also accurately do not grasped at the caste of the earth, approximately calculate 500 ~ 1,000 ten thousand kinds.Wherein, directly the kind relevant with the mankind is about 15, and more than 000 plants, and insect is subject to 21 century maximum untapped living resources with abundant, various kind and attracts attention, and also concentrates applying flexibly of care insect for increasing revenue source in agriculture field.
At present, domestic insect industry is divided into, natural enemy insect field, flowerpot are propagated with insect field, pet study insect field, edible medicinal insect field, red-letter day with insect field etc., recently in the agricultural aquatic food portion of Korea S by delivering " long-range planning 2020 ", the plan of insect industry is cultivated the market scale of more than 7,000 hundred million yuan of South Korean Wons, and insect industry meets the policy direction of government, supporting prediction growth backward according to the cause of government can be very high.
On the other hand, be subject to the huge longicorn (Callipogon relictus) of one of the insect of maximum welcome to belong to coleoptera Cerambycidae with fluent and elegant outward appearance and preciousness, inhabit the jungle of the tall and big trees of growth such as carpinus turczaninowii, Mongolian oak, Fraxinus rhynchophylla.Large 12 centimetres of male body, the large 7-8 cm of female gonosome, body look is black or pitchy, and has strong light tone, and back has dense filemot hair.
Huge longicorn is worth precious insect as biological classification and chorology and is designated as natural monument, and along with the insect museum continued to increase, the trend of reading extensively the insect Industry growth such as guild hall, ecological park, experiential learning shop, the needs of study or pet also increase gradually.
The feed existed to raise your insect light nature uncommon such as huge longicorn is not enough, can the feed of mass rearing insect so must research and develop, and to find out preferred method for breeding be very important.
But, in domestic office of independent state or the investment research institute that there is no research insect resources of Korea S, the research to insect cause is performed in offices such as some universities, public institute of state, research institutes among the people, but only centered by the honeybee of useful insect resource, cicada worm, chafer, butterfly, aquatic insect class, natural enemy insect class, obtain some achievements, to the indoor physiological Study of huge longicorn and ecological research because of also incomplete, so be difficult to prolific fact.
To this, the present invention, in order to overcome the relevant problem of longicorn huge with amount reproduction, the result of effort after deliberation, develops the man-made feeds meeting and raise huge longicorn, and, when raising huge longicorn with this feed, a large amount of production can be guaranteed, thus complete the present invention.
Summary of the invention
The object of the invention is to, provide a kind of can the man-made feeds of the huge longicorn of amount reproduction and manufacture method thereof in indoor.
Another object of the present invention is to, a kind of suitable feeding environment by guaranteeing huge longicorn is provided, not only the larval phase of 5 years to 6 years is foreshortened to 3.8 years, the incubation rate of larva and the eclosion rate of adult can also be improved, and the whole year can the method for breeding of the huge longicorn of amount reproduction.
In order to reach above-mentioned purpose, the invention provides huge longicorn breeding man-made feeds, wherein, to Oak Tree irradiating microwaves at temperature 50 C is to 60 DEG C of infiltration, until dry to moisture be after 1% to 5%, the Oak Tree of oven dry is ground into Oak Tree sawdust, then mixed-powder mixture on Oak Tree sawdust.
Further, the invention provides the manufacture method of huge longicorn breeding man-made feeds, wherein, to Oak Tree irradiating microwaves at temperature 50 C is to 60 DEG C of infiltration, until drying to moisture is after 1% to 5%, pulverize the sawdust producing Oak Tree, then mixed-powder mixture on above-mentioned Oak Tree sawdust.
Oak Tree impregnated in after in water and pulverizes after microwave irradiation process by the present invention, the moisture being fully absorbed in Oak Tree tissue in infiltration process is activated in microwave irradiation process, and trickle damage is caused to Oak Tree histological structure, cause institutional framework changes, Oak Tree can be processed by this process and make huge longicorn can draw Oak Tree better.Further, by above-mentioned microwave irradiation process, the bacterium on rotten Oak Tree, the ovum of fungi or potential ant that huge roundheaded borer is worked the mischief or other insect predators or larva can be removed.
Above-mentioned microwave can use the microwave of any frequency being suitable for water activity, but preferably use is easily absorbed in water and hyperfrequency between the strong 300MHz to 3GHz of sterilizing power, more preferably uses the microwave of the 2540MHz bandwidth identical with the resonant frequency of water.
Any Oak Tree can be used being used in Oak Tree of the present invention, but preferably use rotten suitable Oak Tree, but consider the characteristic of ingesting of huge longicorn, more preferably use the Oak Tree decayed timber that pith portion is not exclusively rotten.Now, use as good after removing the crust of Oak Tree completely." decayed timber " is the tree of being rotted by microorganisms such as mushrooms.
In the process of irradiating microwaves, consider convenience and the efficiency being organized trickle damage by water activity Oak Tree, and the characteristic of ingesting of huge longicorn, preferably use the Oak Tree of the size of long 10cm × diameter 10-20cm.
The Oak Tree of seeping water in the process of irradiating microwaves is that nature is dried, and Oak Tree or the Oak Tree powder of removing moisture are like this easy to keeping because being not easy rotten.
The moisture of the Oak Tree of drying is preferably 1% to 5%, if moisture is lower than 1%, then there is the shortcoming of drying time and expense increase, if more than 5%, then Oak Tree can be easily rotten.
Supplied by the Oak Tree sawdust of the method dried powder cellulose source as huge roundheaded borer.To the not restriction of the pulverized size of above-mentioned Oak Tree sawdust, but conveniently ingest in order to huge longicorn, its size is preferably 50 to 60 meshes.
In huge longicorn breeding man-made feeds of the present invention, consider the equilibrium of the nutritional labeling of above-mentioned huge longicorn and characteristic of ingesting, the weight portion that above-mentioned Oak Tree saw comprises 95 to 97 relative to man-made feeds 100 weight portion is good.
In huge longicorn breeding man-made feeds of the present invention, above-mentioned mixture of powders contains the mineral composition needed for huge longicorn breeding, and above-mentioned mineral composition preferably comprises the mineral matters of raising needed for huge longicorn such as calcium, magnesium, phosphorus, potassium, sodium, iron, manganese, zinc and copper.
Further, above-mentioned Oak Tree can impregnated on vitamin mixed solution and use, and the vitamin now used is ascorbic acid, uric acid, folic acid, pantothenic acid, biotin, vitamin B1, vitamin B2, vitamin B6, inositol.When using vitamin mixed solution to seep water, the vitamin in Oak Tree micro organization is permeated together with moisture, in irradiating microwaves process, by the activation of moisture, disperse equably and be absorbed on Oak Tree, therefore, even if Oak Tree is ground into powder, also because of said vitamin uniform composition be distributed on the powder of Oak Tree, thus can trophism be improved.Further, as the ascorbic acid that said vitamin adds, because acidity is low, therefore, the rotten of man-made feeds can be prevented, anticorrisive agent effect can be played.
Now, said vitamin mixed solution can use with water 300 weight portion be as the criterion add ascorbic acid 0.05 to 0.1 weight portion, urine 0.05 to 0.1 weight portion, folic acid 0.0005 to 0.0015 weight portion, pantothenic acid 0.003 to 0.007 weight portion, biotin 0.0003 to 0.0007 weight portion, vitaminB10 .0003 to 0.0007 weight portion, vitamin B2 0.0005 to 0.0009 weight portion, vitamin B6 0.0006 to 0.001 weight portion and inositol 0.1 to 0.3 weight portion after the vitamin mixed solution that dilutes.
So, mixed-powder mixture on the Oak Tree that irradiating microwaves is dried and the man-made feeds of huge longicorn that manufacture are the progenesis being of value to larva, not only can shorten the institute of larvae extremely between late-maturing larva through the date, the resistance to pathogen etc. can also be improved, thus improve the survival rate of larva.
According to other forms of the present invention, the invention provides the artificial fecundation method of huge longicorn, wherein, comprising: the breeding man-made feeds larvae of huge longicorn being supplied under the environment of temperature 20 DEG C, humidity 60% to above-mentioned huge longicorn, prepare the stage of the whole instar larvae through late-maturing larval phase; And by above-mentioned whole instar larvae nymphosis to stage of cicada pupa.
Specifically, the artificial fecundation method of huge longicorn of the present invention is first prepare the larvae of huge longicorn, then supplies man-made feeds of the present invention to prepare the whole instar larvae through late-maturing larval phase.The larvae of above-mentioned huge longicorn is not particularly limited, but gathers the ovum of huge longicorn on Oak Tree decayed timber, and hatches out on the culturing base using man-made feeds of the present invention.Prepare from above-mentioned larvae during the whole instar larvae of late-maturing larval phase general about 1350 days, now, the raising temperature of larva preferably remains on 18 DEG C to 22 DEG C that are applicable to growth, preferably keeps the constant temperature of 20 DEG C.
Further, in order to promote the food of huge longicorn movable, above-mentioned larvae is separated 1 respectively and is put in vial and raises as good when 1 instar larvae.Above-mentioned vial will regularly wash, sterilizing keeps clean, in case larva obtains disease.
Whole instar larvae through this process peels into cicada pupa through nymphosis process.The above-mentioned nymphosis stage preferably carries out under the condition of temperature 20 DEG C, humidity 60%, and when raising under these conditions, whole instar larvae will remove rest period from, thus between the feeding period that greatly can shorten huge longicorn.
Preferably remain on 60% in the preparation stage of above-mentioned whole instar larvae and the humidity in above-mentioned nymphosis stage, if humidity is more than 60% or lower than when raising under the environment of 60%, the nymphosis rate step-down of larva, therefore, above-mentioned larva is preferably raised under the condition of humidity 60%.
Now, in the stage preparing whole instar larvae, regulate humidity in mode water being directly sprayed onto above-mentioned man-made feeds, in the above-mentioned nymphosis stage, water is directly sprayed onto with it cicada pupa, in order to not be stressed, regulate humidity in the mode of flower mud supply moisture.
And, when supplying moisture in order to above-mentioned humidity regulation, in order to resist the infection of the Mycophyta being harmful to huge longicorn, input antifungal agent can be mixed on a small quantity, the antifungal agent that now can mix is Butoconazole Nitrate, clotrimazole, nitric acid ketoconazole, Miconazole, polyene antifungal agent, nystatin, amphotericin B, pimaricin, Oxiconazole Nitrate, nitric acid terconazole, tioconazole, flutrimazole, Yin health azoles, allylamine, this naphthalene special is fragrant, butenafine, Amorolfine, Naftifine, Portugal's health azoles, azole, econazole, voriconazole (voriconizole), ammonia health azoles, posaconazole, sulconazole, double-benzimidazoles (diction bis-benzimida zoles), glucan synthetic inhibitor, echinocandin-class (echinacandins), anidulafungin, Caspofungin, MFG, dapsone, Ciclopirox Olamine, Haloprogin, Tuo Natan (tolnatane), undecylenate etc.
The above-mentioned nymphosis stage carries out under the photoperiod environment of OL (bright phase), 24D (dark phase).
Supply man-made feeds of the present invention to raise to the larva stored according to the present invention under the photoperiod environment of temperature 20 DEG C, humidity 60%, OL (bright phase), 24D (dark phase), thus, can the larva of artificial incubation and the huge longicorn of nymphosis in indoor.
According to the propagation method of huge longicorn of the present invention, the survival rate of larva significantly improves compared with the survival rate of the larva under natural environment, therefore can improve nymphosis rate and eclosion rate, also can shorten from larvae to late-maturing larva institute through the date.
That is, originally the huge longicorn of artificial feeding not only can not can be bred through the whole year by the present invention, survival rate and the eclosion rate of larva can also be improved, thus can the huge longicorn of amount reproduction every year.
Invention effect
As mentioned above, contain the Oak Tree powder of irradiating microwaves according to the man-made feeds of huge longicorn of the present invention, thus be of value to the progenesis of larva, not only can shorten the institute of larvae extremely between late-maturing larva through the date, the resistance to pathogen can also be improved, thus improve the survival rate of larva.
Further, the rest period of huge longicorn can be removed from according to the present invention, not only annually can breed huge longicorn, and improve survival rate and the eclosion rate of larva, thus the whole year can the huge longicorn of amount reproduction.
Accompanying drawing explanation
Fig. 1 is the flow chart of the method for breeding according to huge longicorn of the present invention.
Detailed description of the invention
Below, the present invention is described in detail by embodiment.The present embodiment is used to illustrate of the present invention, not delimit the scope of the invention.
[Production Example 1] utilizes the Oak Tree powder that impregnated in the vitamin aqueous solution to carry out manufacture of intraocular feed
Remove the completely not rotten long 10cm × diameter 10-20cm Oak Tree crust in pith portion completely, the vitamin aqueous solution soaks 2 hours, then utilize micro-wave oven irradiating microwaves under 60 DEG C of environment to dry Oak Tree until moisture is 3%, then pulverize with disintegrating machine and manufacture Oak Tree powder.
The said vitamin aqueous solution uses at water 300ml mixing ascorbic acid 85.8mg, uric acid 75.3mg, folic acid 0.95mg, pantothenic acid 4.6mg, biotin 0.61mg, vitaminB10 .4mg, vitamin B2 0.67mg, vitamin B6 0.84mg, inositol 186mg.
Calcium mixture 290g, magnesium 70g, phosphorus 150g, potassium 610g, sodium 20g, iron 0.3g, manganese 1.6g, zinc 2.34g and copper 0.2g make powder mixture 1444.44g.
Mix above-mentioned Oak Tree powder 35kg and above-mentioned mixture of powders 1444.44g and produce huge sky cattle breeding employment work feed.
[Production Example 2] does not use the vitamin aqueous solution and uses simple addition manner manufacture of intraocular feed
Replace vitamin aqueous impregnation Oak Tree with water and manufacture Oak Tree powder, and the vitamin same with at the vitamin of above-mentioned Production Example 1 is simply added in Oak Tree powder using.Except above 2, the method identical with above-mentioned Production Example 1 is used to manufacture huge sky cattle breeding employment work feed.
[comparative example] does not use microwave manufacture of intraocular feed
Do not carry out heat drying engineering to after Oak Tree irradiating microwaves, and utilize steam sterilization 15 minutes, then utilize drying machine dry.Except above heat drying engineering, the method identical with above-mentioned Production Example 1 is used to manufacture huge sky cattle breeding employment work feed.
[experimental example 1] adds the development characteristics of vitamin and the huge longicorn through irradiating microwaves process.
In order to observe the development characteristics of adding vitamin and the huge longicorn through irradiating microwaves process, select larvae to test.
Choose the adult 6 (female 3, male 3) at initial stage, adult is carried out mating, the decayed timber of Oak Tree is received after laying eggs and take huge roundheaded borer (82) to carry out artificial feeding.
The larvae selected is supplied the man-made feeds manufactured according to above-mentioned Production Example 1, Production Example 2 and comparative example like this, through the huge roundheaded borer phase of 200 days, is measured the survival rate of larva, with following table 1 show measure larval phase institute through period.
Table 1[table 1]
As above-mentioned table 1, supply experiment group 1-1 and 1-2 and supply through the Production Example 1 of irradiating microwaves process manufacture and the man-made feeds of Production Example 2 without compared with the experiment group 1-3 after the man-made feeds of the comparative example of microwave treatment, through the time point of 200 days, the survival rate of larva is increased to the numerical value of 11% and 8% respectively.
Can find out according to such result, use the man-made feeds efficiency of feed compared with the man-made feeds used without the Oak Tree powder of microwave processing process through the Oak Tree powder of irradiating microwaves processing procedure micro organization damage to be improved, thus the survival rate of larva can be improved.
And, the kind comparative development feature of the aqueous solution used when soaking according to Oak Tree, supply uses the experiment group 1-15 of the man-made feeds of the Production Example 1 of the vitamin aqueous solution and supply to use development characteristics compared with the simple experiment group 1-2 adding the man-made feeds of the Production Example 2 of vitamin to be improved.
Such result is, vitamin in Oak Tree micro organization is permeated in irradiating microwaves process by the activate of moisture together with moisture, disperse equably and be absorbed on Oak Tree, therefore, even if Oak Tree be ground into powder also due to said vitamin uniform composition be distributed on the powder of Oak Tree, thus can trophism be improved.
And, larva is observed through known according to supplied feed kind, supply uses the vitamin aqueous solution, and in the experiment group 1-1 of the man-made feeds of the Production Example 1 of microwave irradiation process, larval phase, is the shortest through the date, supplies larva in the feed experiment group 1-3 of the comparative example of microwave irradiation process the longest through the date.
Comprehensive analysis the above results is known, irradiating microwaves and impregnated in the vitamin aqueous solution and whether play material impact for the feature of huge longicorn man-made feeds and the growth of huge roundheaded borer on Oak Tree decayed timber.
[experimental example 2] establishes the most applicable feeding environment of huge roundheaded borer
2-1 larvae is to the applicable feeding environment of nymphosis process
The man-made feeds showing the Production Example 1 of best feed efficiency in experimental example 1 are utilized to look for the optimal environment of the raising of huge roundheaded borer.
In order to ensure the optimal environment of raising, only form the man-made feeds of 1 group of supply Production Example 1 with huge longicorn larvae 5, raise according to the temperature of following table 2 and damp condition, and observe the nymphosis rate of larva and the change of eclosion rate.
Table 2
| Experiment group | Temperature | Humidity | Nymphosis rate | Eclosion rate |
| 2-1 | 15℃ | 60％ | 40％ | 40％ |
| 2-2 | 18℃ | 60％ | 100％ | 100％ |
| 2-3 | 20℃ | 60％ | 100％ | 100％ |
| 2-4 | 22℃ | 60％ | 80％ | 80％ |
| 2-5 | 25℃ | 60％ | 20％ | 20％ |
| 2-6 | 20℃ | 40％ | 40％ | 80％ |
| 2-7 | 20℃ | 50％ | 80％ | 80％ |
| 2-8 | 20℃ | 70％ | 80％ | 80％ |
| 2-9 | 20℃ | 80％ | 40％ | 80％ |
As shown in Table 2 above, observe that humidity is same is set as 60%, and the experiment group 2-1 to 2-5 of variations in temperature is known, the larva raised under the environment of temperature 18 DEG C and 20 DEG C is all by nymphosis, under raising temperature is low to moderate the environment of 15 DEG C, nymphosis rate is for dropping to 40%, it is somely drop to 80% that raising temperature rises to nymphosis rate under the environment of 22 DEG C, and under temperature being risen to the environment of 25 DEG C, its nymphosis rate sharply drops to 20%.
Observe that temperature is same is set as 20 DEG C again, and the experiment group 2-3 and 2-6 to 2-9 of humidity change, be as the criterion with humidity 60%, along with the increase and decrease nymphosis rate of humidity is continuous decrease.
Based on the above results, in order to improve the nymphosis rate of huge roundheaded borer, under the temperature conditions of 18 DEG C to 22 DEG C, preferably keeping the damp condition of 50% to 70% to raise, under the temperature conditions of 20 DEG C, most preferably keep the damp condition of 60% to raise.Further, from the result of above-mentioned table 2, as long as the huge roundheaded borer of nymphosis substantially 100% can be sprouted wings.
the whole instar larvae of 2-2 is to the applicable feeding environment of nymphosis
When raising huge longicorn, the stage of most critical is the stage entering nymphosis process from whole instar larvae, carries out as follows to find this experiment of feeding environment the most suitable.
By the man-made feeds of the whole instar larvae 5 of huge longicorn composition 1 group of supply Production Example 1, according to raise under the temperature of following table 3 and damp condition, and observe between the nymphosis rate of larva, prepupal period, the change of eclosion rate.
Table 3
| Experiment group | Temperature | Humidity | Nymphosis rate | * between prepupal period | Eclosion rate |
| 3-1 | 15℃ | 60％ | 40％ | 20.9 days | 40％ |
| 3-2 | 18℃ | 60％ | 100％ | 15.7 days | 100％ |
| 3-3 | 20℃ | 60％ | 100％ | 13.4 days | 100％ |
| 3-4 | 22℃ | 60％ | 80％ | 16.5 days | 80％ |
| 3-5 | 25℃ | 60％ | 20％ | 18.4 days | 20％ |
| 3-6 | 20℃ | 40％ | 40％ | 16.7 days | 80％ |
| 3-7 | 20℃ | 50％ | 80％ | 14.3 days | 80％ |
| 3-8 | 20℃ | 70％ | 80％ | 17.6 days | 80％ |
| 3-9 | 20℃ | 80％ | 40％ | 15.7 days | 80％ |
* the number of days from whole instar larvae to nymphosis is referred between prepupal period.
From above-mentioned table 3, according to the nymphosis rate of the whole instar larvae of rearing conditions and eclosion rate identical with the nymphosis rate of the larvae of above-mentioned experimental example 2-1 and the result of variations of eclosion rate, but have difference between prepupal period.
That is, observe whole instar larvae by between the prepupal period of nymphosis, under the condition of temperature 20 DEG C, humidity 60% period be 13.4 days the shortest, along with the increase and decrease of temperature or damp condition, period also increases.Can find out that whole instar larvae reacts according to the sensitive of rearing conditions with such result, also can find out that the most applicable rearing conditions can removing the nymphosis of the whole instar larvae of the rest period of huge longicorn from is temperature 20 DEG C, humidity 60%.
[experimental example 3] establishes the photoperiod condition being applicable to huge roundheaded borer and raising
Under above-mentioned huge roundheaded borer shows the condition (20 DEG C, 60%) of the highest nymphosis rate and eclosion rate, investigation is applicable to the photoperiod that huge roundheaded borer is raised.
In order to establish the photoperiod condition being applicable to raising, by the larvae 5 of huge longicorn composition 1 group, the man-made feeds of supply Production Example 1, observe the nymphosis rate of larva and the change of eclosion rate after raising with the photoperiod condition of following table 4.
Table 4
As shown in Table 4 above, the all larvas throwing in long day experiment group 4-1 to 4-3 under condition of light for one day more than 12 hours are got killed extremely, the dead rate of getting killed also observing short day larva under condition of throwing in light for one day 6 hours is high, the experiment group 4-5 larva raised under dark phase condition in one day 24 hours is all by nymphosis, and the larva of nymphosis is all sprouted wings.
Can find out that the huge longicorn of nymphosis is for best under the photoperiod condition of 0L24D.
Claims (12)
1. the manufacture method of huge longicorn breeding man-made feeds, containing Oak Tree sawdust and mixture of powders in described man-made feeds, it is characterized in that, to Oak Tree irradiating microwaves at humidity 50 DEG C to 60 DEG C of infiltration, until dry to moisture be after 1% to 5%, the Oak Tree of oven dry is ground into Oak Tree sawdust, then mixed-powder mixture on above-mentioned Oak Tree sawdust.
2. the manufacture method of huge longicorn breeding man-made feeds according to claim 1, is characterized in that, above-mentioned Oak Tree sawdust is relative to the weight portion of man-made feeds 100 weight portion mixing 95 to 97.
3. the manufacture method of huge longicorn breeding man-made feeds according to claim 1, it is characterized in that, contain in above-mentioned mixture of powders the mineral composition needed for huge longicorn breeding, above-mentioned mineral composition selects more than one in calcium, magnesium, phosphorus, potassium, sodium, iron, manganese, zinc and copper.
4. the manufacture method of huge longicorn breeding man-made feeds according to claim 1, it is characterized in that, the Oak Tree of above-mentioned infiltration impregnated in vitamin mixed solution.
5. the manufacture method of huge longicorn breeding man-made feeds according to claim 4, it is characterized in that, said vitamin mixed solution comprises the more than one vitamin be selected from ascorbic acid, uric acid, folic acid, pantothenic acid, biotin, vitamin B1, vitamin B2, vitamin B6, inositol.
6. the manufacture method of huge longicorn breeding man-made feeds according to claim 5, it is characterized in that, said vitamin mixed solution is for be as the criterion with water 300 weight portion, add ascorbic acid 0.05 to 0.1 weight portion, uric acid 0.05 to 0.1 weight portion, folic acid 0.0005 to 0.0015 weight portion, pantothenic acid 0.003 to 0.007 weight portion, biotin 0.0003 to 0.0007 weight portion, vitaminB10 .0003 to 0.0007 weight portion, vitamin B2 0.0005 to 0.0009 weight portion, the vitamin mixed solution of vitamin B6 0.0006 to 0.001 weight portion and the dilution of inositol 0.1 to 0.3 weight portion.
7. an artificial fecundation method for huge longicorn, is characterized in that, comprising:
Under the environment of temperature 18 DEG C to 20 DEG C, humidity 50% to 70%, raise the larvae of huge longicorn with the huge longicorn breeding man-made feeds by the method manufacture according to any one of claim 1 to 6, prepare the stage of the whole instar larvae through late-maturing larval phase; And
By above-mentioned whole instar larvae nymphosis to stage of cicada pupa.
8. the artificial fecundation method of huge longicorn according to claim 7, is characterized in that, above-mentioned larvae for when 1 instar larvae respectively 1 be separated to vial raise.
9. the artificial fecundation method of huge longicorn according to claim 7, is characterized in that, the above-mentioned nymphosis stage carries out under the condition of temperature 20 DEG C, humidity 60%.
10. the artificial fecundation method of huge longicorn according to claim 9, it is characterized in that, preparing the stage of above-mentioned whole instar larvae, regulating humidity in mode water being directly sprayed onto above-mentioned man-made feeds, in the above-mentioned nymphosis stage, to regulate humidity at flower mud for the mode fed water.
The artificial fecundation method of 11. huge longicorns according to claim 7, is characterized in that, the above-mentioned nymphosis stage carries out under the photoperiod condition of 0L24D.
12. 1 kinds of huge longicorn breeding man-made feeds, described man-made feeds comprise Oak Tree sawdust and mixture of powders, it is characterized in that, to above-mentioned Oak Tree irradiating microwaves at temperature 50 C is to 60 DEG C of infiltration, until drying to moisture is after 1% to 5%, the Oak Tree of oven dry is ground into Oak Tree sawdust.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020120054975A KR101222469B1 (en) | 2012-05-23 | 2012-05-23 | Methods of making artificial diet for callipogon relictus, and mass breeding callipogon relictus using thereof |
| KR10-2012-0054975 | 2012-05-23 | ||
| PCT/KR2013/004362 WO2013176441A1 (en) | 2012-05-23 | 2013-05-16 | Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same |
Publications (2)
| Publication Number | Publication Date |
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| CN104334031A true CN104334031A (en) | 2015-02-04 |
| CN104334031B CN104334031B (en) | 2016-06-22 |
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| CN201380027010.8A Expired - Fee Related CN104334031B (en) | 2012-05-23 | 2013-05-16 | For the man-made feeds of the huge Anoplophorae seu Aprionae of amount reproduction, man-made feeds manufacture method and utilize these man-made feeds raise huge Anoplophorae seu Aprionae method |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JP2015520610A (en) |
| KR (1) | KR101222469B1 (en) |
| CN (1) | CN104334031B (en) |
| CA (1) | CA2874428C (en) |
| RU (1) | RU2605749C2 (en) |
| WO (1) | WO2013176441A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123691A (en) * | 2015-09-10 | 2015-12-09 | 王志刚 | Longicorn phagostimulant, application and method thereof applied for disturbing host recognition behavior of longicorn, and longicorn insecticide |
| CN110692600A (en) * | 2018-07-10 | 2020-01-17 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feed for anoplophora chinensis larvae and subculturing method of anoplophora chinensis |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430183B (en) * | 2013-09-13 | 2017-06-16 | 广西壮族自治区林业科学研究院 | Large-scale breeding and method for breeding in a kind of greater wax moth room |
| CN104381221B (en) * | 2014-10-11 | 2017-01-25 | 中国农业科学院植物保护研究所 | Bactrocera dorsalis large-scale feeding method |
| CN104982390A (en) * | 2015-07-11 | 2015-10-21 | 云南农业大学 | Method for efficiently culturing queen bees of west bees by using incubator |
| CN110402898B (en) * | 2019-08-16 | 2021-10-19 | 云南省热带作物科学研究所 | Method for obtaining standard test insects of rhaponticum uniflorum |
| CN113907051B (en) * | 2021-12-03 | 2022-11-04 | 遵义市林业科学研究所 | Artificial breeding method of zanthoxylum bungeanum and tiger longicorn |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN104334031B (en) | 2016-06-22 |
| RU2015101919A (en) | 2016-08-10 |
| KR101222469B1 (en) | 2013-01-15 |
| CA2874428A1 (en) | 2013-11-28 |
| JP2015520610A (en) | 2015-07-23 |
| WO2013176441A1 (en) | 2013-11-28 |
| RU2605749C2 (en) | 2016-12-27 |
| CA2874428C (en) | 2018-02-13 |
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