CN104334031B - For the man-made feeds of the huge Anoplophorae seu Aprionae of amount reproduction, man-made feeds manufacture method and utilize these man-made feeds raise huge Anoplophorae seu Aprionae method - Google Patents
For the man-made feeds of the huge Anoplophorae seu Aprionae of amount reproduction, man-made feeds manufacture method and utilize these man-made feeds raise huge Anoplophorae seu Aprionae method Download PDFInfo
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- CN104334031B CN104334031B CN201380027010.8A CN201380027010A CN104334031B CN 104334031 B CN104334031 B CN 104334031B CN 201380027010 A CN201380027010 A CN 201380027010A CN 104334031 B CN104334031 B CN 104334031B
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- VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 description 1
- 229960002894 oxiconazole nitrate Drugs 0.000 description 1
- WVNOAGNOIPTWPT-NDUABGMUSA-N oxiconazole nitrate Chemical compound O[N+]([O-])=O.ClC1=CC(Cl)=CC=C1CO\N=C(C=1C(=CC(Cl)=CC=1)Cl)/CN1C=NC=C1 WVNOAGNOIPTWPT-NDUABGMUSA-N 0.000 description 1
- 150000004291 polyenes Chemical class 0.000 description 1
- 229960001589 posaconazole Drugs 0.000 description 1
- RAGOYPUPXAKGKH-XAKZXMRKSA-N posaconazole Chemical compound O=C1N([C@H]([C@H](C)O)CC)N=CN1C1=CC=C(N2CCN(CC2)C=2C=CC(OC[C@H]3C[C@@](CN4N=CN=C4)(OC3)C=3C(=CC(F)=CC=3)F)=CC=2)C=C1 RAGOYPUPXAKGKH-XAKZXMRKSA-N 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229960002607 sulconazole Drugs 0.000 description 1
- 239000012622 synthetic inhibitor Substances 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 229960004214 tioconazole Drugs 0.000 description 1
- 229940075466 undecylenate Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 229960004740 voriconazole Drugs 0.000 description 1
- BCEHBSKCWLPMDN-MGPLVRAMSA-N voriconazole Chemical compound C1([C@H](C)[C@](O)(CN2N=CN=C2)C=2C(=CC(F)=CC=2)F)=NC=NC=C1F BCEHBSKCWLPMDN-MGPLVRAMSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/32—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from hydrolysates of wood or straw
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Physiology (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Insects & Arthropods (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Inorganic Chemistry (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of study for the huge Anoplophorae seu Aprionae of amount reproduction man-made feeds, man-made feeds manufacture method and utilize these man-made feeds raise huge Anoplophorae seu Aprionae method for breeding。Man-made feeds according to the huge Anoplophorae seu Aprionae of the present invention contain the Oak Tree powder of irradiating microwaves, make to be of value to the progenesis of larva, such that it is able to shorten the institute from larvae to ripe larva through the date, and by improving the resistance to pathogenic bacteria etc., the viability of larva can be effectively improved。That is, according to the present invention originally can not the huge Anoplophorae seu Aprionae of artificial breeding, not only can breed the whole year, also by the raw rate and the eclosion rate that improve larva, energy amount reproduction。
Description
Technical field
The present invention relates to a kind of for the man-made feeds of the huge Anoplophorae seu Aprionae of amount reproduction, man-made feeds manufacture method and utilize these man-made feeds raise huge Anoplophorae seu Aprionae method。
Background technology
At present, breed at the caste of the earth but without accurately being grasped, about calculate 500～10,000,000 kinds。Wherein, direct relevant with mankind kind is about more than 15,000 kinds, and insecticide is subject to 21 century maximum untapped living resources attracts attention with abundant, various kind, and is also used for increasing applying flexibly of revenue source concentration care insecticide in agriculture field。
At present, domestic insecticide industry is divided into, natural enemy insecticide field, flowerpot are propagated with insecticide field, house pet study insecticide field, edible medicinal insecticide field, red-letter day with insecticide field etc., recently in the agricultural aquatic food portion of Korea S by delivering " long-range planning 2020 ", insecticide industry plan is cultivated the market scale of more than 700,000,000,000 yuan of South Korean Wons, and insecticide industry meets the policy direction of government, supporting prediction growth backward according to the cause of government can be significantly high。
On the other hand, it is belonging to coleoptera Cerambycidae with the huge Anoplophorae seu Aprionae (Callipogonrelictus) of one of fluent and elegant outward appearance and the preciousness insecticide being subject to maximum welcome, inhabits carpinus turczaninowii, Quercus mongolica, Fraxinus rhynchophylla etc. and grow the jungle of tall and big trees。Big 12 centimetres of male body, the big 7-8 cm of female gonosome, body color is black or pitchy, and has strong light tone, and back has close filemot hair。
Huge Anoplophorae seu Aprionae is worth precious insecticide as biological classification and chorology and is designated as natural monument, and along with the trend of the insecticide museum continued to increase, insecticide Industry growth such as review guild hall, ecological park, experiential learning shop etc., study or house pet need also be gradually increased。
The feedstuff existed to raise your insecticide light nature uncommon such as huge Anoplophorae seu Aprionae is not enough, thus have to research and develop can the feedstuff of mass rearing insecticide, and to find out preferred method for breeding be particularly important。
But, in the domestic office of independent state that there is no research insect resources of Korea S or investment research institute, the research to insecticide cause is performed in offices such as some universities, public institute of state, institutes among the people, but only centered by the Apis of useful insect resource, Cicadae worm, chafer, butterfly, aquatic insect class, natural enemy insect class, obtain some achievements, to the indoor physiological Study of huge Anoplophorae seu Aprionae and ecological research because of also incomplete, so being difficult to prolific fact。
To this, the present invention in order to overcome the problem relevant with the huge Anoplophorae seu Aprionae of amount reproduction, the result of effort after deliberation, develop and meet the man-made feeds raising huge Anoplophorae seu Aprionae, and, when raising huge Anoplophorae seu Aprionae with this feedstuff, it can be ensured that produce in a large number, thus completing the present invention。
Summary of the invention
It is an object of the invention to, it is provided that a kind of can the man-made feeds of the huge Anoplophorae seu Aprionae of amount reproduction and manufacture method thereof in indoor。
Another object of the present invention is to, there is provided a kind of by assuring that the suitable feeding environment of huge Anoplophorae seu Aprionae, not only the larval phase of 5 years to 6 years, is foreshortened to 3.8 years, it is also possible to improve the eclosion rate of the incubation rate of larva and adult, and the whole year can the method for breeding of the huge Anoplophorae seu Aprionae of amount reproduction。
In order to reach above-mentioned purpose, the present invention provides huge Anoplophorae seu Aprionae breeding man-made feeds, wherein, Oak Tree irradiating microwaves at temperature 50 C to 60 DEG C to infiltration, until dry to moisture be after 1% to 5%, the Oak Tree of drying is ground into Oak Tree sawdust, then mixed-powder mixture on Oak Tree sawdust。
Further, the present invention provides the manufacture method of huge Anoplophorae seu Aprionae breeding man-made feeds, wherein, Oak Tree irradiating microwaves at temperature 50 C to 60 DEG C to infiltration, until drying to moisture is after 1% to 5%, pulverize the sawdust producing Oak Tree, then mixed-powder mixture on above-mentioned Oak Tree sawdust。
Oak Tree be impregnated in and pulverizes after microwave irradiation processes after in water by the present invention, infiltration process fully absorbs the moisture at Oak Tree tissue be activated in microwave irradiation process, and Oak Tree histological structure is caused trickle damage, cause organizational structure changes, Oak Tree can be processed by this process and make huge Anoplophorae seu Aprionae can draw Oak Tree better。Further, processed by above-mentioned microwave irradiation, it is possible to remove ovum or the larva of the antibacterial on rotten Oak Tree, fungus or the potential Formica fusca that huge roundheaded borer is worked the mischief or other insect predators。
Above-mentioned microwave can use the microwave of any frequency being suitable for water activity, but the hyperfrequency that be easily absorbed in water and sterilizing power strong 300MHz to 3GHz between is preferably used, and more preferably uses the microwave of the 2540MHz bandwidth identical with the resonant frequency of water。
Any Oak Tree can be used the Oak Tree being used in the present invention, but rotten suitable Oak Tree is preferably used, but consider the characteristic of ingesting of huge Anoplophorae seu Aprionae, more preferably use the Oak Tree decayed timber that pith portion is not exclusively rotten。Now, use after removing the crust of Oak Tree completely and be preferred。" decayed timber " is the tree rotted by microorganisms such as mushrooms。
In the process of irradiating microwaves, it is contemplated that convenience with by the efficiency of the water activity trickle damage of Oak Tree tissue, and the characteristic of ingesting of huge Anoplophorae seu Aprionae, it is preferred to use the Oak Tree of the size of long 10cm × diameter 10-20cm。
In the process of irradiating microwaves, the Oak Tree of infiltration is that nature is dried, and Oak Tree or the Oak Tree powder of so removing moisture are prone to keeping because being not easy rotten。
The moisture of the Oak Tree of drying is preferably 1% to 5%, if moisture is lower than 1%, then there is drying time and the shortcoming of expense increase, if more than 5%, then Oak Tree can be easily rotten。
Supplied as the cellulose source of huge roundheaded borer by the Oak Tree sawdust of the method dried powder。Pulverized size not restriction to above-mentioned Oak Tree sawdust, but in order to huge Anoplophorae seu Aprionae conveniently ingests, it is preferably sized to 50 to 60 meshes。
In the huge Anoplophorae seu Aprionae breeding man-made feeds of the present invention, it is contemplated that the equilibrium of the nutritional labeling of above-mentioned huge Anoplophorae seu Aprionae and characteristic of ingesting, the weight portion that above-mentioned Oak Tree saw comprises 95 to 97 relative to man-made feeds 100 weight portion is preferred。
In the huge Anoplophorae seu Aprionae breeding man-made feeds of the present invention, above-mentioned mixture of powders contains the mineral composition that the breeding of huge Anoplophorae seu Aprionae is required, and above-mentioned mineral composition preferably comprises calcium, magnesium, phosphorus, potassium, sodium, ferrum, manganese, zinc and copper etc. and raises the mineral needed for huge Anoplophorae seu Aprionae。
Further, above-mentioned Oak Tree can impregnated in and use on vitamin mixed solution, and the vitamin now used is ascorbic acid, uric acid, folic acid, pantothenic acid, biotin, vitamin B1, vitamin B2, vitamin B6, inositol。When using vitamin mixed solution to seep water, the vitamin in Oak Tree micro organization is permeated together with moisture, in irradiating microwaves process, by the activation of moisture, it is uniformly dispersed and absorbs on Oak Tree, therefore, even if Oak Tree is ground into powder, also because said vitamin composition is evenly distributed on the powder of Oak Tree, it is thus possible to improve trophism。Further, as the ascorbic acid added on said vitamin, owing to acidity is low, accordingly it is possible to prevent man-made feeds is rotten, it is possible to play preservative effect。
Now, said vitamin mixed solution can use to be as the criterion with water 300 weight portion and add the vitamin mixed solution of dilution behind ascorbic acid 0.05 to 0.1 weight portion, urine 0.05 to 0.1 weight portion, folic acid 0.0005 to 0.0015 weight portion, pantothenic acid 0.003 to 0.007 weight portion, biotin 0.0003 to 0.0007 weight portion, vitaminB10 .0003 to 0.0007 weight portion, vitamin B2 0.0005 to 0.0009 weight portion, vitamin B6 0.0006 to 0.001 weight portion and inositol 0.1 to 0.3 weight portion。
So, mixed-powder mixture on the Oak Tree that irradiating microwaves is dried and the man-made feeds of huge Anoplophorae seu Aprionae that manufacture are the progenesis being of value to larva, not only can shorten larvae to the institute between late-maturing larva through the date, moreover it is possible to improve the resistance to pathogen etc., thus improving the survival rate of larva。
Other forms according to the present invention, the present invention provides the artificial fecundation method of huge Anoplophorae seu Aprionae, wherein, including: to the larvae of huge Anoplophorae seu Aprionae temperature 20 DEG C, humidity 60% environment under supply the breeding man-made feeds of above-mentioned huge Anoplophorae seu Aprionae, prepare the stage of whole instar larvae through late-maturing larval phase；And the stage by above-mentioned whole instar larvae nymphosis to Cicadae pupa。
Specifically, the artificial fecundation method of the huge Anoplophorae seu Aprionae of the present invention is first prepare the larvae of huge Anoplophorae seu Aprionae, and the man-made feeds then supplying the present invention prepare the whole instar larvae through late-maturing larval phase。The larvae of above-mentioned huge Anoplophorae seu Aprionae is not particularly limited, but gathers the ovum of huge Anoplophorae seu Aprionae on Oak Tree decayed timber, and hatches out on the culturing base of man-made feeds using the present invention。Preparing from above-mentioned larvae through the period of the whole instar larvae of late-maturing larval phase general about 1350 days, now, the raising temperature of larva preferably remains in 18 DEG C to 22 DEG C that are suitable for growth, it is preferable that keep the constant temperature of 20 DEG C。
Further, in order to the food promoting huge Anoplophorae seu Aprionae is movable, above-mentioned larvae is individually separated 1 and is put in vial and raises is preferred when 1 instar larvae。Above-mentioned vial regularly to wash, maintenance cleaning of sterilizing, in case larva obtains disease。
Whole instar larvae through this process peels into Cicadae pupa through nymphosis process。Carrying out when the above-mentioned nymphosis stage is preferably in temperature 20 DEG C, humidity 60%, when raising under these conditions, whole instar larvae will remove rest period from, such that it is able to during being greatly shortened the raising of huge Anoplophorae seu Aprionae。
60% is preferably remained in the humidity preparing stage of above-mentioned whole instar larvae and above-mentioned nymphosis stage, if humidity is more than 60% or lower than when raising under the environment of 60%, the nymphosis rate step-down of larva, therefore, raise when above-mentioned larva is preferably in humidity 60%。
Now, in the stage preparing whole instar larvae, in the way of water is directly sprayed onto above-mentioned man-made feeds, regulate humidity, in the above-mentioned nymphosis stage, water is directly sprayed onto with Cicadae pupa, in order to not be stressed, to regulate humidity in the way of flower mud supply moisture。
And, when supplying moisture for above-mentioned humidity regulation, in order to resist the infection of the Mycophyta being harmful to huge Anoplophorae seu Aprionae, input antifungal can be mixed on a small quantity, the antifungal that now can mix is Nitric acid butoconazole, clotrimazole, nitric acid ketoconazole, miconazole, polyene antifungal agent, nystatin, amphotericin B, pimaricin, Oxiconazole Nitrate, nitric acid terconazole (triaconazole), tioconazole, flutrimazole, Yin health azoles, allylamine, this naphthalene special is fragrant, butenafine, amorolfine, naftifine, Portugal's health azoles, azole, econazole, voriconazole (voriconizole), ammonia health azoles, posaconazole, sulconazole, double-benzimidazoles (dictionbis-benzimidazoles), glucosan synthetic inhibitor, echinocandin-class (echinacandins), anidulafungin, Caspofungin, MFG, dapsone, ciclopirox olamine, haloprogin, Tuo Natan (tolnatane), undecylenate etc.。
The above-mentioned nymphosis stage be OL (bright phase), 24D (dark phase) photoperiod environment under carry out。
Temperature 20 DEG C, humidity 60%, OL (bright phase), 24D (dark phase) photoperiod environment under to store according to the present invention larva supply the present invention man-made feeds raise, thus, can the larva of artificial incubation and the huge Anoplophorae seu Aprionae of nymphosis in indoor。
The propagation method of the huge Anoplophorae seu Aprionae according to the present invention, the survival rate of larva significantly improves compared with the survival rate of the larva under natural environment, therefore can improve nymphosis rate and eclosion rate, it is also possible to shorten the institute from larvae to late-maturing larva through the date。
That is, originally can not the huge Anoplophorae seu Aprionae of artificial breeding not only can be bred through the whole year by the present invention, it is also possible to improve survival rate and the eclosion rate of larva, such that it is able to the annual huge Anoplophorae seu Aprionae of amount reproduction。
Invention effect
As mentioned above, the man-made feeds of the huge Anoplophorae seu Aprionae according to the present invention contain the Oak Tree powder of irradiating microwaves, thus being of value to the progenesis of larva, not only can shorten larvae to the institute between late-maturing larva through the date, the resistance to pathogen can also be improved, thus improving the survival rate of larva。
Further, the rest period of huge Anoplophorae seu Aprionae can be removed from according to the present invention, not only annual can breed huge Anoplophorae seu Aprionae, and improve survival rate and the eclosion rate of larva, thus, the whole year can the huge Anoplophorae seu Aprionae of amount reproduction。
Accompanying drawing explanation
Fig. 1 is the flow chart of the method for breeding of the huge Anoplophorae seu Aprionae according to the present invention。
Detailed description of the invention
Hereinafter, the present invention is described in detail by embodiment。The present embodiment is used to illustrate the present invention, not delimit the scope of the invention。
[manufacturing example 1] utilizes the Oak Tree powder that impregnated in vitamin aqueous solution to carry out manufacture of intraocular feedstuff
Remove long 10cm × diameter 10-20cm Oak Tree crust that pith portion is completely not rotten completely, vitamin aqueous solution soaks 2 hours, then utilize microwave oven irradiating microwaves under 60 DEG C of environment to dry Oak Tree until moisture is 3%, then pulverize with disintegrating machine and manufacture Oak Tree powder。
Said vitamin aqueous solution uses at water 300ml mixing ascorbic acid 85.8mg, uric acid 75.3mg, folic acid 0.95mg, pantothenic acid 4.6mg, biotin 0.61mg, vitaminB10 .4mg, vitamin B2 0.67mg, vitamin B6 0.84mg, inositol 186mg。
Calcium mixture 290g, magnesium 70g, phosphorus 150g, potassium 610g, sodium 20g, ferrum 0.3g, manganese 1.6g, zinc 2.34g and copper 0.2g manufacture mixture of powders 1444.44g。
Mix above-mentioned Oak Tree powder 35kg and above-mentioned mixture of powders 1444.44g and produce huge sky cattle breeding employment work feedstuff。
[manufacturing example 2] does not use vitamin aqueous solution to use simple addition manner manufacture of intraocular feedstuff
Replace vitamin aqueous impregnation Oak Tree with water and manufacture Oak Tree powder, and the vitamin same with the vitamin in above-mentioned manufacture example 1 is simply added in Oak Tree powder use。Outside upper 2, the method identical with above-mentioned manufacture example 1 is used to manufacture huge sky cattle breeding employment work feedstuff。
[comparative example] does not use microwave manufacture of intraocular feedstuff
To not being thermally dried engineering after Oak Tree irradiating microwaves, and utilize steam to sterilize 15 minutes, then utilize drying machine to dry。Except above heat drying engineering, the method identical with above-mentioned manufacture example 1 is used to manufacture huge sky cattle breeding employment work feedstuff。
[experimental example 1] adds vitamin and the development characteristics of the huge Anoplophorae seu Aprionae through irradiating microwaves process。
In order to observe the development characteristics adding vitamin and the huge Anoplophorae seu Aprionae through irradiating microwaves process, larvae is selected to test。
Choose the adult 6 (female 3, male 3) at initial stage, adult is carried out copulation, the decayed timber of Oak Tree is received and after laying eggs, takes huge roundheaded borer (82) to carry out artificial breeding。
Supplying according to above-mentioned manufacture example 1 on the larvae so selected, manufacture example 2 and the man-made feeds of comparative example manufacture, through the huge roundheaded borer phase of 200 days, measure the survival rate of larva, table 1 below display measures the institute of larval phase through period。
Table 1[table 1]
Such as above-mentioned table 1, be fed past irradiating microwaves process the manufactures example 1 manufactured and manufacture example 2 man-made feeds test group 1-1 and 1-2 and supply without compared with the experiment group 1-3 after the man-made feeds of the comparative example of microwave treatment, through the time point of 200 days, the survival rate of larva increases respectively to the numerical value of 11% and 8%。
Can be seen that according to such result, the man-made feeds using the Oak Tree powder through the damage of irradiating microwaves processing procedure micro organization are improved without the efficiency of feedstuff compared with the man-made feeds of the Oak Tree powder of microwave processing process with using, it is thus possible to improve the survival rate of larva。
And, the kind comparative development feature of the aqueous solution used when soaking according to Oak Tree is it can be seen that supply uses the experiment group 1-15 of the man-made feeds of the manufacture example 1 of vitamin aqueous solution development characteristics compared with the experiment group 1-2 supplying the man-made feeds using the simple manufacture example 2 adding vitamin to be improved。
Such result is, the vitamin in Oak Tree micro organization is permeated in irradiating microwaves process by the activation of moisture together with moisture, it is uniformly dispersed and absorbs on Oak Tree, therefore, even if Oak Tree is ground into powder also due to said vitamin composition is evenly distributed on the powder of Oak Tree, it is thus possible to improve trophism。
And, larva is observed through known according to the feedstuff kind supplied, supply uses vitamin aqueous solution, and in the experiment group 1-1 of the man-made feeds of the manufacture example 1 of microwave irradiation process, larval phase, is the shortest through the date, supplies larva in the feedstuff experiment group 1-3 of the comparative example without microwave irradiation process the longest through the date。
Comprehensive analyze the above results it can be seen that on Oak Tree decayed timber irradiating microwaves and impregnated in whether vitamin aqueous solution plays material impact for the feature of huge Anoplophorae seu Aprionae man-made feeds and the growth of huge roundheaded borer。
What [experimental example 2] established huge roundheaded borer is best suitable for feeding environment
2-1 larvae is to the applicable feeding environment of nymphosis process
The optimal environment of the raising to huge roundheaded borer looked for by the man-made feeds utilizing the manufacture example 1 showing best feed efficiency in experimental example 1。
In order to ensure raising optimal environment, only forming 1 group of supply with huge Anoplophorae seu Aprionae larvae 5 and manufacture the man-made feeds of example 1, temperature and damp condition according to table 2 below are raised, and observe the nymphosis rate of larva and the change of eclosion rate。
Table 2
| Experiment group | Temperature | Humidity | Nymphosis rate | Eclosion rate |
| 2-1 | 15℃ | 60% | 40% | 40% |
| 2-2 | 18℃ | 60% | 100% | 100% |
| 2-3 | 20℃ | 60% | 100% | 100% 5--> |
| 2-4 | 22℃ | 60% | 80% | 80% |
| 2-5 | 25℃ | 60% | 20% | 20% |
| 2-6 | 20℃ | 40% | 40% | 80% |
| 2-7 | 20℃ | 50% | 80% | 80% |
| 2-8 | 20℃ | 70% | 80% | 80% |
| 2-9 | 20℃ | 80% | 40% | 80% |
As shown in Table 2 above, observe that humidity is same is set as 60%, and the experiment group 2-1 to 2-5 of variations in temperature is known, the larva raised under the environment of temperature 18 DEG C and 20 DEG C is all by nymphosis, raising temperature is low to moderate under the environment of 15 DEG C nymphosis rate for dropping to 40%, it is some drop to 80% that raising temperature rises to nymphosis rate under the environment of 22 DEG C, and its nymphosis rate under the environment of 25 DEG C that temperature risen to sharply drops to 20%。
Observe that temperature is same is set as 20 DEG C again, and the experiment group 2-3 and 2-6 to 2-9 of humidity change, it is as the criterion with humidity 60%, along with the increase and decrease nymphosis rate of humidity is continuous decrease。
Based on the above results, in order to improve the nymphosis rate of huge roundheaded borer, it is preferable that keep the damp condition of 50% to 70% to raise under the temperature conditions of 18 DEG C to 22 DEG C, it is most preferred that keep the damp condition of 60% to raise under the temperature conditions of 20 DEG C。Further, as long as by the result of above-mentioned table 2 it can be seen that the huge roundheaded borer of nymphosis is substantially 100% can to sprout wings。
2-2 instar larvae at end is to the applicable feeding environment of nymphosis
When raising huge Anoplophorae seu Aprionae, the stage of most critical is the stage entering nymphosis process from whole instar larvae, is carried out as follows to find this experiment of feeding environment the most suitable。
The whole instar larvae 5 of huge Anoplophorae seu Aprionae is formed 1 group of supply and manufactures the man-made feeds of example 1, raise according at the temperature of table 3 below and damp condition, and during observing the nymphosis rate of larva, propupa, the change of eclosion rate。
Table 3
| Experiment group | Temperature | Humidity | Nymphosis rate | Propupa period * | Eclosion rate |
| 3-1 | 15℃ | 60% | 40% | 20.9 my god | 40% |
| 3-2 | 18℃ | 60% | 100% | 15.7 my god | 100% |
| 3-3 | 20℃ | 60% | 100% | 13.4 my god | 100% |
| 3-4 | 22℃ | 60% | 80% | 16.5 my god | 80% |
| 3-5 | 25℃ | 60% | 20% | 18.4 my god | 20% |
| 3-6 | 20℃ | 40% | 40% | 16.7 my god | 80% |
| 3-7 | 20℃ | 50% | 80% | 14.3 my god | 80% |
| 3-8 | 20℃ | 70% | 80% | 17.6 my god | 80% |
| 3-9 | 20℃ | 80% | 40% | 15.7 my god | 80% |
* the number of days from whole instar larvae to nymphosis is referred to during propupa。
By above-mentioned table 3 it can be seen that nymphosis rate and eclosion rate according to rearing conditions instar larvae at end are identical with the result of variations of the nymphosis rate of the larvae of above-mentioned experimental example 2-1 and eclosion rate, but during propupa, there is difference。
That is, observe whole instar larvae by during the propupa of nymphosis, when temperature 20 DEG C, humidity 60% period be 13.4 days the shortest, along with the increase and decrease of temperature or damp condition, period also increases。Can be seen that whole instar larvae reacts according to the sensitive of rearing conditions with such result, it is also possible to the rearing conditions finding out the nymphosis of the whole instar larvae of the rest period being best suitable for removing huge Anoplophorae seu Aprionae from is temperature 20 DEG C, humidity 60%。
[experimental example 3] is established and is suitable for the photoperiod condition that huge roundheaded borer is raised
Under the condition (20 DEG C, 60%) that above-mentioned huge roundheaded borer shows the highest nymphosis rate and eclosion rate, investigation is suitable for the photoperiod that huge roundheaded borer is raised。
In order to establish the photoperiod condition being suitable for raising, the larvae 5 of huge Anoplophorae seu Aprionae being formed 1 group, supply manufactures the man-made feeds of example 1, observes the nymphosis rate of larva and the change of eclosion rate after raising according to the photoperiod condition of table 4 below。
Table 4
As shown in Table 4 above, all larvas of the experiment group 4-1 to 4-3 when the long day throwing in light for more than 12 hours in one day are got killed extremely, also observe when the short day throwing in light in one day 6 hours that the dead rate of getting killed of larva is high, the experiment group 4-5 larva raised when the dark phase in one day 24 hours is all by nymphosis, and the larva of nymphosis is all sprouted wings。
Can be seen that the huge Anoplophorae seu Aprionae of nymphosis is the best when the photoperiod of 0L 24D。
Claims (12)
1. the manufacture method of huge Anoplophorae seu Aprionae breeding man-made feeds, containing Oak Tree sawdust and mixture of powders in described man-made feeds, it is characterized in that, Oak Tree irradiating microwaves at the temperature of 50 DEG C to 60 DEG C to infiltration, until dry to moisture be after 1% to 5%, the Oak Tree of drying is ground into Oak Tree sawdust, then mixed-powder mixture on above-mentioned Oak Tree sawdust。
2. the manufacture method of huge Anoplophorae seu Aprionae breeding man-made feeds according to claim 1, it is characterised in that above-mentioned Oak Tree sawdust mixes the weight portion of 95 to 97 relative to man-made feeds 100 weight portion。
3. the manufacture method of huge Anoplophorae seu Aprionae breeding man-made feeds according to claim 1, it is characterized in that, containing the mineral composition that the breeding of huge Anoplophorae seu Aprionae is required in above-mentioned mixture of powders, above-mentioned mineral composition is selected more than one in calcium, magnesium, phosphorus, potassium, sodium, ferrum, manganese, zinc and copper。
4. the manufacture method of huge Anoplophorae seu Aprionae breeding man-made feeds according to claim 1, it is characterised in that the Oak Tree of above-mentioned infiltration impregnated in vitamin mixed solution。
5. the manufacture method of huge Anoplophorae seu Aprionae breeding man-made feeds according to claim 4, it is characterised in that said vitamin mixed solution comprises selected from ascorbic acid, uric acid, folic acid, pantothenic acid, biotin, vitamin B1, vitamin B2, vitamin B6, more than one vitamin in inositol。
6. the manufacture method of huge Anoplophorae seu Aprionae breeding man-made feeds according to claim 5, it is characterized in that, said vitamin mixed solution, for be as the criterion with water 300 weight portion, adds ascorbic acid 0.05 to 0.1 weight portion, uric acid 0.05 to 0.1 weight portion, folic acid 0.0005 to 0.0015 weight portion, pantothenic acid 0.003 to 0.007 weight portion, biotin 0.0003 to 0.0007 weight portion, vitamin B10.0003 to 0.0007 weight portion, vitamin B20.0005 to 0.0009 weight portion, vitamin B6The vitamin mixed solution of 0.0006 to 0.001 weight portion and the dilution of inositol 0.1 to 0.3 weight portion。
7. the artificial fecundation method of a huge Anoplophorae seu Aprionae, it is characterised in that including:
Temperature 18 DEG C to 20 DEG C, humidity 50% to 70% environment under, with the larvae being raised huge Anoplophorae seu Aprionae by the huge Anoplophorae seu Aprionae breeding man-made feeds of the method manufacture according to any one of claim 1 to 6, preparation is through the stage of the whole instar larvae of late-maturing larval phase；And
Stage by above-mentioned whole instar larvae nymphosis to Cicadae pupa。
8. the artificial fecundation method of huge Anoplophorae seu Aprionae according to claim 7, it is characterised in that above-mentioned larvae is raised for 11 instar larvae is put into vial。
9. the artificial fecundation method of huge Anoplophorae seu Aprionae according to claim 7, it is characterised in that the above-mentioned nymphosis stage carries out when temperature 20 DEG C, humidity 60%。
10. the artificial fecundation method of huge Anoplophorae seu Aprionae according to claim 9, it is characterized in that, in the stage preparing above-mentioned whole instar larvae, in the way of water is directly sprayed onto above-mentioned man-made feeds, regulate humidity, in the above-mentioned nymphosis stage, to regulate humidity at flower mud in the way of feeding water。
11. the artificial fecundation method of huge Anoplophorae seu Aprionae according to claim 7, it is characterised in that the above-mentioned nymphosis stage is to carry out when the photoperiod of 0L 24D。
12. huge Anoplophorae seu Aprionae breeding man-made feeds, described man-made feeds comprise Oak Tree sawdust and mixture of powders, it is characterised in that to above-mentioned Oak Tree irradiating microwaves at the temperature of 50 DEG C to 60 DEG C of infiltration, until drying to moisture is after 1% to 5%, the Oak Tree of drying is ground into Oak Tree sawdust。
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| KR1020120054975A KR101222469B1 (en) | 2012-05-23 | 2012-05-23 | Methods of making artificial diet for callipogon relictus, and mass breeding callipogon relictus using thereof |
| KR10-2012-0054975 | 2012-05-23 | ||
| PCT/KR2013/004362 WO2013176441A1 (en) | 2012-05-23 | 2013-05-16 | Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same |
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| CN104430183B (en) * | 2013-09-13 | 2017-06-16 | 广西壮族自治区林业科学研究院 | Large-scale breeding and method for breeding in a kind of greater wax moth room |
| CN104381221B (en) * | 2014-10-11 | 2017-01-25 | 中国农业科学院植物保护研究所 | Bactrocera dorsalis large-scale feeding method |
| CN104982390A (en) * | 2015-07-11 | 2015-10-21 | 云南农业大学 | Method for efficiently culturing queen bees of west bees by using incubator |
| CN105123691B (en) * | 2015-09-10 | 2017-07-11 | 王志刚 | A kind of longicorn phagostimulant, its application, its be used to disturbing the host of longicorn to recognize the method and a kind of longicorn insecticide of behavior |
| CN110692600B (en) * | 2018-07-10 | 2022-04-19 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feed for anoplophora chinensis larvae and subculturing method of anoplophora chinensis |
| CN110402898B (en) * | 2019-08-16 | 2021-10-19 | 云南省热带作物科学研究所 | Method for obtaining standard test insects of rhaponticum uniflorum |
| CN113907051B (en) * | 2021-12-03 | 2022-11-04 | 遵义市林业科学研究所 | Artificial breeding method of zanthoxylum bungeanum and tiger longicorn |
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| CA2874428C (en) | 2018-02-13 |
| CN104334031A (en) | 2015-02-04 |
| WO2013176441A1 (en) | 2013-11-28 |
| CA2874428A1 (en) | 2013-11-28 |
| RU2015101919A (en) | 2016-08-10 |
| JP2015520610A (en) | 2015-07-23 |
| KR101222469B1 (en) | 2013-01-15 |
| RU2605749C2 (en) | 2016-12-27 |
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