CA2874428C

CA2874428C - Artificial feed for mass culture of long-horned beetles, method for manufacturing same, and breeding method using same

Info

Publication number: CA2874428C
Application number: CA2874428A
Authority: CA
Inventors: Dae Am Yi
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-05-23
Filing date: 2013-05-16
Publication date: 2018-02-13
Anticipated expiration: 2033-05-16
Also published as: CN104334031B; RU2015101919A; KR101222469B1; CA2874428A1; JP2015520610A; CN104334031A; WO2013176441A1; RU2605749C2

Abstract

The present invention relates to artificial feed for the mass culture of long-horned beetles, to a method for manufacturing the artificial feed, and to a method for breeding long-horned beetles using same. The artificial feed for long-horned beetles according to the present invention contains oak powder irradiated with microwaves, and thus improves the initial development of larvae, decreases the larval age gap between hatched larvae and full-grown larvae, and improves the larval survival rate by increasing resistance to pathogenic bacteria and the like. In other words, according to the present invention, long-horned beetles which normally cannot be artificially bred can be bred year-round, and long-horned beetles can be bred in large quantities by increasing the larval survival rate and emergence rate.

Description

ARTIFICIAL FEED FOR MASS CULTURE OF LONG-HORNED BEETLES, METHOD FOR MANUFACTURING
SAME, AND BREEDING METHOD USING SAME
Technical Field [1] The present invention relates to an artificial feed for the mass culture of long-horned beetles, to a method for manufacturing the artificial feed, and to a method for growing long-horned beetles using same.
io Background Art

[2] The total number of the species of insects living on the earth is not accurately known, but it may be estimated that 5 million to 10 million species of the insects are living on the earth. It is reported that the number of the species who are directly or indirectly related to the human being may be about 15,000 species, and the insects receive attention as biological resources biggest in the 21st century along with a variety of enough specie diversities.
In the agricultural sector, there is a bit attention in the application of insects as a source for increasing the income of the farm.
.[3] The insects-related industry in Korea may be divided into an insect field for natural enemy, an insect field for pollen medium, an insect field for study and pets, an insect field for foods and medicines, an insect field for feasts, etc. In recent years, it was announced that the ministry for food, agriculture, forestry and fisheries of Korea had planned to grow the insect industry up to the market scale of more than 7 hundred billion won in the 2020 in "Vision 2020". It is expected that the insect industry would grow greatly thanks to the business support from the government in conformity with the direction of the governmental policy.
[4] Meanwhile, a long-horned beetle (Callipogon relictus), who is one of the most popular insects thanks to its beautiful exterior and rarity, is an insect belonging to Coleoptera and lives forest where old and tall read-leaved io hornbeam, mongolian oak, ash tree, etc. grow. The long-horned beetle is 12cm long in case of a male and is 7-8cm long in case of a female. The long-horned beetle has a black or dark brown body and has dense gloss and light brown fine hairs on its back.
[5] The demand for such insects for the sake of learning or pets Is gradually increases in conformity with the trend of the growth of insect business, for example, the long-horned beetle is a very rare insect who have organism classification and distribution science-based values and is designated as a natural monument and is being protected, and an exhibition, an exposition, an ecological park, an experiential learning place, etc. which all use insects 20 gradually increase.
[6] In order to feed a rare insect such as a long-horned beetle, since the feed of insects who exist in the natural system is insufficient, the technology development such as the development of feed which will be used for the sake of mass growth of such insects is necessary, and a way for the optimized raising method for the targeted insects becomes an important issue.
[7] In Korea, there however are not any independent national organization or government-assisted research organization which were set up for the sake of insect resource recovery study, and only part of universities, national and pubic research centers, private research centers, etc. is currently conducting the .insect-based business; however in the useful insect resource development, some performance results from bee, white grub, blue polished i 0 chafer, butterfly, aquatic insect, natural enemy insect, etc., but an indoor biological research and ecological research of the long-horned beetle is incomplete, so the mass culture of the long-horned beetle is hard.
[8] As a result of the effort to improve the problems related to the mass culture of the long-horned beetles, the inventor of the present invention has confirmed that the long-horned beetles might be mass-cultured if the long-horned beetles are bred in such a way that an artificial feed optimized for the growth of the long-horned beetles is developed and is fed to the long-horned beetles, and then has invented the claimed invention.
Disclosure of Invention [9] Accordingly, it is an object of the present invention to provide an artificial feed for an indoor mass culture of long-horned beetles and a

3 method for manufacturing the same.
[10] It is another object of the present invention through set a proper growth environment not only that greatly shorten the larvaperiod from 5 to 6 years to 3.8 years in natural condition, but also enhance the larvae hatching rate and adult eclosion rate, and so to provide a method for growing, in large quantities, long-horned beetles all the year round.
[11] To achieve the above objects, there is provided an artificial feed for growing long-horned beetles, which comprises oak sawdust and a powder mixture wherein the oak sawdust is prepared in such a way that water-to soaked oak is irradiated with microwaves at 50 C to 60 C and is dried until moisture content becomes 1% to 5% and is crushed into powder.
112] In addition, there is a method for preparing artificial feed for growing long-horned beetles wherein oak sawdust is prepared in such a way that water-soaked oak is irradiated with microwaves at 50 C to 60 C and is Is dried until moisture content becomes 1% to 5% and is crushed, and a powder mixture is added to the thusly prepared oak sawdust.
113] The oak of the present invention is soaked with water and irradiated with microwaves and is crushed. The moisture enough soaked into the tissue of the oak during the water-soaking procedure is activated during the 20 irradiation of the microwaves, so minute damages occurs in the tissue structure of the oak, and the tissue structures are changed for thereby preparing, through the above procedures, the oak which may be easily eaten by the long-horned

4 beetles. In addition, through the irradiation process procedure of the microwaves, it is possible to additionally obtain effects of eliminating bacteria and fungus which exist in the conky oak or eggs or larva of the ants or predacious insects which might become competitors against the larva of the long-horned beetles.
114] In case of the microwaves, any frequency of microwave is available as far as it is proper for the activation of moisture. It is preferred that microwaves of 300Hz to 3GHz which may be well absorbed by water and may have strong sterilization effects is used. It is more preferred that microwaves of io 2540MHz having the same bandwidth as the resonance frequency of water is used.
[15] In case of the oak used in the present invention, any type of oak may be used. It is preferred that properly rotten oak is used. It is more preferred that the conky oak the core of which is not fully rotten is used in consideration of the feeding characteristics of the long-horned beetles. At this time, it is preferred to completely peel off the outer skin of the oak. Here, the conky wood represents the wood rotten by microorganism such as mushroom, etc.
[16] It is preferred that the oak has a length of 10cm and a diameter of 10-.12cm in consideration of any convenience during the irradiation of microwaves, the minute damage efficiency of the tissues of the oak through the moisture activation and the feeding characteristics of the long-horned

5 beetles.
[17] The oak water-soaked during the irradiation of the microwaves may be naturally dried. Since the thusly moisture-eliminated oak or oak powder is not easily rotten, the preservation may be improved.
[18] When drying the oak, it is preferred that the moisture content is 1%-5%. If the moisture content is less than 1%, the drying time and cost disadvantageously increase, and if higher than 5%, the oak may be easily =
rotten.
[19] The thusly dried and powdered oak sawdust is supplied as to the cellulose source of the larva of the long-horned beetles. The sizes of the crushed oak sawdust are not limited, but it is preferred that the sizes thereof are 50 to 60 meshes for the sake of comfort feeding of the long-horned beetles.
[20] It is preferred that in the artificial feed for growing the long-horned beetles according to the present invention, the oak sawdust contains 95 to 97 parts by weight with respect to 100 parts by weight of the artificial feed in consideration of the balance and feeding characteristics of the nutrition components of the long-horned beetles.=
[21] In the artificial feed for growing the long-horned beetles according to the present invention, the powder mixture contains mineral components which are necessary for growing the long-horned beetles. It is preferred that such mineral component comprises minerals necessary for growing the long-horned beetles such as calcium, magnesium, phosphorus,

6 potassium, sodium, iron, manganese, zinc and copper.
[22] In addition, the oak may be used after it is soaked in vitamin-mixed solution. The vitamin used then is ascorbic acid, urea, folate, pantothenic acid, biotin, vitamin B1, vitamin B2, vitamin 66 or inositol. In case of the oak is soaked using the vitamin-mixed solution, the vitamin penetrated along with the moisture into the minute tissues of the oak may be evenly distributed and absorbed by the oak through the activation operation of the moisture during the irradiation of the microwaves, so even when the oak is crushed into powder, the vitamin components are evenly distributed in the oak powder for thereby io improving nourishment. In addition, the ascorbic acid added as vitamin component may play a role of antiseptic which prevents the decomposition of the artificial feed thanks to its low acidity.
[23] At this time, the vitamin-mixed solution is a vitamin-mixed solution diluted by adding, with respect to 300 parts by weight, 0.05 to 0.1 parts by weight of ascorbic acid, 0.05 to 0.1 parts by weight of urea, 0.0005 to 0.0015 parts by weight of folate, 0.003 to 0.007 parts by weight of pantothenic acid, 0.0003 to 0.0007 parts by weight of biotin, 0.0003 to 0.0007 parts by weight of vitamin B1, 0.0005 to 0.0009 parts by weight of vitamin B2, 0.0006 to 0.001 parts by weight of vitamin B6 and 0.1 to 0.3 parts by weight of inositol.
=[24] The artificial feed for long-horned beetles prepared by mixing the powder mixture to the oak which is dried by irradiating microwaves improves the initial growing of the larva, by which it is possible to shorten the

7 larva duration period from the hatched larva to the full-grown larva and improve the survival rate of the larva by increasing resistance against pathogenic bacteria.
[25]
[26] According to an aspect of the present invention, the present invention provides a method for artificially growing long-horned beetles comprising preparing the last instar larva, who has lived as a full-grown larva, by supplying, to the latched larva of the long-horned beetles, the artificial feed for growing long-horned beetles under a condition of a temperature of 20 C and io humidity of 60%
and growing the larva, and pupating the stored last instar larva into pupa.
[27] More specifically, in the method for artificially growing long-horned beetles according to the present invention, the hatched larva of the long-horned beetles are prepared, and the last instar larva, who has lived as a full-grown larva, is prepared by supplying the artificial feed of the present invention.
The hatched larva of the long-horned larva are not limited, but the larva may be hatched in a culture medium which uses the artificial feed of the present invention after the eggs of the long-horned beetles are collected from the conky oak. The duration for which the last instar larva, who have lived from the hatched larva to the full-grown larva, is prepared is about 1,350 days. At this time, it is preferred that the temperature of the larva is maintained at 18 to 22 which is proper for the growing of the larva, and the constant temperature condition of 20 is maintained.
[28] In addition, it is preferred that the hatched larva are separated one by one and are grown in glass bottles so as to improve the eating activity of the long-horned beetles when the hatched larva are 1 year.
It is also preferred that the larva should not get a disease by maintaining cleanness in such a way to ash, disinfect or exchange the glass bottle at predetermined time intervals.
[29] The thusly prepared last instar larva becomes pupa through the pupation procedure. It is preferred that the step of the pupation is performed 0 under a condition of a temperature of 20 C and humidity of 60%. In case that the larva is grown under such a condition, it is possible to greatly reduce the growth period of the long-horned beetles by eliminating the dormancy period of the last instar larva.
130] It is preferred to maintain humidity at 60% during the preparations of the last instar larva and the pupation. In case that the larva are grown at higher or lower than 60% of humidity, the pupation rate of the larva is decreased, so it is most preferred that the larva are grown under a condition of humidity of 60%.
= 131] At this time, the adjustment of humidity in the step for preparing the last instar larva is performed by directly spraying water onto the artificial feed, and the adjustment of humidity of the step of the pupation is performed by directly spraying water onto the bodies of the pupas, and in order to eliminate any causes for which stress occurs, moisture is supplied to floral foam.
[32] In addition, when moisture is supplied for the sake of adjustment of humidity, it is possible to add a small amount of antifungal agent so as to prevent any infection from fungus which is harmful to the long-horned beetles. At this time, the mixed antifungal agent is butoconazole nitrate, clotrimazole, ketoconazole nitrate, miconazole, antifungal polyene, Nystatin, amphotericin B, Pimaricin, Oxiconazole nitrate, terconazole nitrate, tioconazole, flutrimazole, intraconizole, Ally!amine, terbenafine, Butenafine, Amorolfine, Naftifine, gluconazole, azole, Econazole, Voriconazole, fluconazole, posaconazole, sulconazole, diction bis-benzimidazoles, glucan-combined inhibitor, echinacandins, Anidulafungin, caspofungin, micafugin, diaphenylsulfone, ciclopiroxolamine, haloprogin, tolnatane, and undecylenate.
[33] The step of the pupation may be performed under a photoperiod environment of OL(bright stage), 24D (dark stage).
[34] When growing the larva stored according to the present invention under an environment of a temperature of 20 C and humidity of 60%
and a photoperiod environment of OL (bright stage) and 240 (dark stage) while feeding the artificial feed of the present invention to the larva, it is possible to artificially indoors pupate and emerge the larva of the long-horned beetles.
[35] According to the method for growing the long-horned beetles of the present invention, the survival rate of the larva may be much more increased as compared with the survival rate of in natural states, so the pupation rate and the emergence rate may be significantly increased, and the larva duration periods from the hatched larva to the full-grown larva may be shortened. Namely, according to the present invention, it is possible to breed all the year round the long-horned beetles which was impossible to artificially breed in the conventional art, and the survival rate and the pupation rate of the larva may be increased, so the long-horned beetles may be artificially grown in large quantities all the year round.
[36]
ro Advantageous effects [37] As mentioned earlier, the artificial feed for long-horned beetles according to the present invention is characterized in that the period of the growth of larva from a hatched larva to a full-grown larva may be shortened in such a way the initial development of the larva is improved because the artificial feed for long-horned contains oak powder irradiated with microwave, and it is possible to improve the survival rate of the larva by enhancing resistance to pathogenic bacteria.
[38] In addition, the long-horned beetles can be grown all the year round by eliminating a period of dormancy, and it is possible to breed in large quantities the long-horned beetles by increasing the survival rate and emergence rate of larva.

Brief Description of Drawings [39] Figure 1 is a flow chart illustrating a method for growing long-horned beetles.
Best modes for carrying out the invention [40] The present invention will be described in more detail along with the exemplary embodiments. The exemplary embodiments are provided for only illustrative purposes and should not be interpreted like the scope of the present invention might be limited by such exemplary embodiments.
[41]
[42] [Manufacture Example 1] Manufacture of artificial feed using vitamin aqueous solution-soaked oak powder [43] The outer skin of the oak whose core was not completely rotten and which had a dimension of a length of 10cm x a diameter of 10-12cm was fully removed and was soaked in vitamin aqueous solution for 2 hours. The oak was heated and dried in such a way that it was irradiated with microwaves using a microwave range at 60 C until it had 3% of moisture. Thereafter, the oak was crushed using a crusher for thereby manufacturing oak powder.
[44] The vitamin aqueous water was prepared by adding, to 300mg of water, 85.8mg of ascorbic acid, 75.3mg of urea, 0.95mg of folate, 4.6mg of pantothenic acid, 0.61mg of biotin, 0.4mg of vitamin B1, 0.67mg of vitamin B2, 0.84mg of vitamin B6 and 186mg of inositol.
[45] 1444.44g of the powder mixture was prepared by mixing 290g of calcium, 70g of magnesium, 150g of phosphorus, 610g of potassium, 20g of sodium, 0.3g of iron, 1.6g of manganese, 2.34g of zinc and 0.2g of copper.
[46] The artificial feed for growing long-horned beetles was prepared by mixing 35kg of the oak powder and 1444.44g of the powder mixture.
[47]
[48] [Manufacture Example 2] Manufacture of artificial feed by simply adding vitamin, not in a form of aqueous solution [49] The oak powder was prepared using the water-soaked oak using water, instead of the vitamin aqueous solution. The vitamin was obtained in such a way that the vitamin same as the vitamin used in the manufacture example 1 was simply added to the oak powder. Except for the above mentioned two points, the artificial feed for growing the long-horned beetles was manufactured by the same method as the manufacture example 1.
[50]
[51] [Comparison Example] Manufacture of artificial feed, not using microwaves [52] Instead of the heating and drying process of the oak by the irradiation of microwaves, the oak was sterilized for 15 minutes using vapor and was dried using a drier. Except for the above mentioned heating and drying process, the artificial feed for growing the long-horned beetles was manufactured by the same method as the manufacture example 1.
[53]
[54] [Experiment Example 1] Growing characteristics of the long-horned beetles based on the addition of vitamin and the irradiation of microwaves [55] The hatched larva was selected and used for the experiment in order to check the growing characteristics of the long-horned beetles based on the addition of vitamin and the irradiation of microwaves.
[56] The hatched larva were obtained in such a way that 6 adult imago (3 imago of female and 3 imago of male) were obtained at the initial stage and io were mated to lay eggs in the conky oak, and the larva (82 larva) of the long-horned beetles were collected and artificially hatched.
[57] The artificial feed manufactured by the manufacture examples 1 and 2 and the comparison example were fed to the thusly selected hatched larva, and the larva survival rates were measured at the time 200 days of the larva of the long-horned beetles elapsed, and the larva duration period was measured. A result of the measurement is shown in the table 1 below.

[58] Table 1 ILarva Experiment Kinds of Feeds Survival Duration of Larva(days) Groups Rate(%) Artificial Feed of 1-1 Manufacture 98 1347 Example 1 Artificial Feed of 1-2 Manufacture 95 1373 Example 2 Artificial Feed of 1-3 Comparison 87 1665 Example [59] As seen in Table 1, the experiment groups 1-1 and 1-2 fed with the artificial feeds of the manufacture examples 1 and 2 manufactured through the microwave-irradiated process procedure showed that the larva survival rates were increased by 11% and 8% at the time 200 days elapsed as compared with the experiment group 1-3 fed with the artificial feed of the comparison example which was not processed with the microwaves.
[60] As a result, the artificial feeds manufactured using the powder of the oak whose tissues are minutely damaged through the microwave irradiation process procedure come to have improved feed efficiencies as compared with the artificial feeds manufactured using the oak powder which was not irradiated with the microwaves, which results in improved larva survival rates.
[61] In addition, when comparing the growing characteristics based on the kinds of the aqueous solutions used when soaking the oak in water. it was known that the experiment group 1-1 fed with the artificial feed of the manufacture example 1 manufactured using the vitamin aqueous solution = showed the more improved growing characteristics as compared with the experiment group 1-1 fed with the artificial feed of the manufacture example 2 simply added with vitamin.
[62] It is considered that the above result was obtained because the vitamin components which penetrated into the minute tissues of the oak along with moisture were evenly distributed and absorbed into the oak through the activation operation of moisture during the irradiation procedure of the microwaves. Thereafter, even though the oak was crushed into powder, the vitamin components remained evenly distributed in the oak powder for thereby improving nutrition.
[63] For the larva duration based on the kinds of the fed feed, it was observed that the experiment group 1-1 fed with the artificial feed of the manufactured example 1 irradiated with the microwaves showed the shortest duration period of the larva, and the experiment group 1-3 fed with the feeds of the comparison example not irradiated with the microwaves showed the longest larva duration.
[64] As a result of the comprehensive analysis based on the above results, the irradiation process of the microwaves of the conky oak and the soaking in the .vitamin aqueous solution were key factors which affected the = CA 02874428 2016-08-09 feed characteristics of the artificial feeds of the long-horned beetles and the growing of the larva of the long-horned beetles.
[65]
[66] Establishment of Proper Growth Condition Environment of Larva of Long-horned Beetles [67] 2-1.. Proper Growth Environment from Hatched Larva to Pupation [68] In the experiment example 1, the experiment for searching for a proper growth environment condition of the larva of the long-horned beetles was performed using the artificial feed of the manufacture example 1 where showed to the highest feed efficiency.
[69] In =order to establish the proper growth environment condition, 5 hatched larva of the long-horned beetles were grouped as one set, and the artificial feed of the manufacture example 1 was fed, and the larva were grown based on the temperature and humidity condition of Table 2, and any change in the pupation rate and the emergence rate of the larva were observed.

[70] Table 2 Experiment Temperature Humidity Pupation rate Emergence group 2-1 15 C 60% 40% 40%
2-2 18 C 60% 100% 100%
2-3 = 20 C 60% 100% 100%
2-4 22 C 60% 80% 80%
2-5 25 C 60% 20% 20%
2-6 20 C 40%
140% go%
2-7 .20 C 50% go% 80%
2-8 20 C 70% go% 80%
2-9 20 C 80% 40% go%
[71] As seen in Table 2, when observing the experiment groups 2-1 to 2-5 wherein the humidity was fixed at 60%, and the temperature was changed, the larva grown at 18 C and 20 C were all pupated, and when the temperature was lowered to 15 C, the pupation rate (40%) was decreased, and when the temperature was increased to 22 C, the pupation rate (80%) was slightly decreased, and then when the temperature was increased to 25 C, the population rate was sharply decreased to 20%.
[72] In addition, when observing the experiment groups 2-3 and 2-6 to to 2-9 wherein the temperature was fixed at 20 C, and the humidity was changed, . as the humidify was increased from the criteria humidity of 60%, it was observed that the pupation rate was gradually decreased.
[73] Based on the above result, in order to improve the pupation rate of the larva of the long-horned beetles, it was preferred to breed the larva while maintaining the temperature conditions at 18 C to 22 C and the humidity conditions at 50% to 70%, and it was most preferred to breed the larva while maintaining the temperature condition at 20 C and the humidity condition at 60%. In addition, as seen from the result of Table 2, it was observed that almost 100% of the larva of the long-horned beetles, who were once pupated, emerged.
[74] 2-2.Proper Growth Environment from Last instar larva to Pupation [75] The following experiment was performed in order to search for the optimized growth environment in the step where the last instar larva started pupating, the stage of which was the most important stage during the growth of the long-horned beetles.
[76] 5 last instar larva of the long-horned beetles were grouped as one set, and the artificial feed of the manufacture example 1 was fed, and the last instar larva were grown based on the temperature and humidity condition of Table 3, and any changes in the pupation rate, the exclusive period and emergence rate of the larva were observed.

[77] Table 3 lExperiment Pupation Exclusive Emergence Temperature Humidity Group Rate Period* Rate 3-1 15 C 60% 40% 20.9 days 40%
3-2 18 C 60% 100% 15.7 days 100%
3-3 20 C 60% 100% 13.4 days 100%
3-4 22 C 60% 80% 16.5 days 80%
3-5 25 C 60% 20% 18.4 days 20%
3-6 20 C 40% 40% 16.7 days 80%
3-7 20 C 50% 80% 14.3 days 80%
3-8 20 C 70% 80% 17.6 days 80%
3-9 20 C 80% 40% 15.7 days 80%
[78] * The exclusive period represents the days from the last instar larva to the pupation..
[79] As seen in the above Table 3, the pupation rate and the emergence rate of the last instar larva based on the growth condition were the same as the results of the changes in the pupation rate and the emergence rate of the hatched larva of the experiment example 2-1, and there was not any change in the exclusive period.
[80] Namely, when observing the exclusive period from the last instar io larva to the pupation, the exclusive period under the condition of 20 C
of = CA 02874428 2016-08-09 temperature and 60% of humidity was the shortest 13.4 days. As the temperature or humidity conditions were increased or decreased, the exclusive period was increased. From the above result, it was observed that the last instar larva was sensitively reacted based on the change in the growth condition, and it was known that the growth condition optimized for the pupation of the last instar larva so as to eliminate the resting stage of the long-horned beetles was 20 C and 60% humidity.
[81]
[82] [Experiment Example 3] Photoperiod Condition Establishment ir) proper to the Growing of Larva of Long-horned Beetles [83] The photoperiod condition proper for the growth of larva of long-horned beetles was searched for under the condition (20 C, 60%) where the larva of long-horned beetles had the highest pupation rate and the emergence rate.
[84] 5 hatched larva of long-horned beetles were grouped as one set so as to establish the photoperiod condition proper for the growth, and the artificial feed of the manufacture example 1 was fed to the larva, and the larva were grown based on the photoperiod condition of Table 4, and the pupation rate and the emergency rate of the larva were observed.

[85] Table 4 Experiment Photoperiod Emergence Pupation Rate(%) Group Condition Rate(%) 4-2 " 181_=6D
4-3 12L=12D
4-4 61...18D 20% 20%
4-5 OL=24D 100% 100%
[86] As seen in Table 4, all the larva of the experiment groups 4-1 to 4-3 under the long-day condition where light was shone more than 12 hours a day were died, and it was observed that the death rate of the larva under the single condition where light was shone for 6 hours a day was very high. In the experiment group 4-5 grown under in the dark space for 24 hours a day, all the larva were pupated, and all the pupated larva emerged.
[87] In conclusion, it is most preferred that the long-horned beetles are pupated under the photoperiod condition of OL-24D.

Claims

Claims:

1. A method for manufacturing an artificial feed for mass culture of long-horned beetles, which contains oak sawdust and a powder mixture, comprising preparing oak sawdust in such a way that microwaves are irradiated to water-soaked oak at a temperature of 50°C to 60°C, and the water-soaked oak is dried until it has moisture content of 1% to 5% and is crushed, and mixing the powder mixture, containing mineral components necessary for the growth of long-horned beetles, with the oak sawdust.

2. The method of claim 1, wherein the oak sawdust is mixed to have 95 to 97 parts by weight with respect to 100 parts by weight of the artificial feed

3. The method of claim 1, wherein the mineral component is one or more than one component selected from a group consisting of calcium, magnesium, phosphorus, potassium, sodium, iron, manganese, zinc and copper

4 The method of claim 1, wherein the water-soaked oak is immersed in a vitamin-mixed solution.

5. The method of claim 4, wherein the vitamin-mixed solution contains urea and at least one vitamin selected from a group consisting of ascorbic acid, folate, pantothenic acid, biotin, vitamin B1, vitamin B2, vitamin B6 and inositol.

6. The method of claim 5, wherein the vitamin-mixed solution is a vitamin-mixed solution diluted by adding, with respect to 300 parts by weight of water, 0.05 to 0 1 parts by weight of ascorbic acid, 0 05 to 0.1 parts by weight of urea, 0.0005 to 0.0015 parts by weight of folate, 0,003 to 0 007 parts by weight of pantothenic acid, 0 0003 to 0.0007 parts by weight of biotin, 0 0003 to 0.0007 parts by weight of vitamin B1, 0.0005 to 0.0009 parts by weight of vitamin B2, 0.0006 to 0.001 parts by weight of vitamin B6 and 0.1 to 0.3 parts by weight of inositol.

7. A method for artificially growing long-horned beetles, comprising:
preparing the last instar beetles, who has lived as a full-grown beetle, bred by feeding the artificial feed for the long-horned beetles prepared by the method of any one of claims 1 to 6 to the hatched beetles of the long-horned beetles under a condition of a temperature of 18°C to 22°C and at a humidity of 50% to 70%, and pupating the thusly prepared last instar beetles into a pupa

8. The method of claim 7, wherein the hatched beetles are one year beetles grown in a glass bottle.

9. The method of claim 7, wherein the step of the pupation is performed under a condition of a temperature of 20°C and a humidity of 60%.

10. The method of claim 9, wherein the adjustment of the humidity of the step for preparing the last instar beetles is performed by spraying water onto the artificial feed, and the adjustment of the humidity of the step of the pupation is performed by supplying moisture onto floral form

11. The method of claim 7, wherein the step of the pupation is performed under a photoperiod environment of 0L.cndot.24D.

12. An artificial feed for long-horned beetles, comprising oak sawdust; and a powder mixture, containing mineral components necessary for the breeding of long-horned beetles, wherein the oak sawdust is a powder type sawdust which is prepared in such a way that water-soaked oak is irradiated with microwaves at a temperature of 50°C to 60°C and is dried until the moisture content becomes 1% to 5% and is crushed into powder.