CN102217729B - Semi-artificial feed for anoplophora chinensis larvae and artificial feeding method - Google Patents

Semi-artificial feed for anoplophora chinensis larvae and artificial feeding method Download PDF

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CN102217729B
CN102217729B CN2011101052137A CN201110105213A CN102217729B CN 102217729 B CN102217729 B CN 102217729B CN 2011101052137 A CN2011101052137 A CN 2011101052137A CN 201110105213 A CN201110105213 A CN 201110105213A CN 102217729 B CN102217729 B CN 102217729B
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longicorn beetle
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蔡守平
黄金水
何学友
曾丽琼
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FUJIAN ACADEMY OF FORESTRY
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Abstract

The invention provides a semi-artificial feed for anoplophora chinensis larvae and a preparation method thereof, as well as an artificial feeding method for the anoplophora chinensis larvae. The semi-artificial feed comprises the following ingredients in parts by weight: 50 to 60 parts of saw dust, 25 to 30 parts of soybean powder, 25 to 30 parts of wheat bran, 2 to 3 parts of yeast powder, 2 to 3 parts of casein, 0.5 to 1 part of food preservative, 5 to 10 parts of agar and 130 to 170 parts of water. The preparation method comprises the steps of mixing, stirring and sterilizing the raw materials; the artificial feeding method for the anoplophora chinensis larvae comprises the steps of collection and feeding of ova and larvae. The semi-artificial feed provided by the invention has the advantages of comprehensive nutritions, low cost, simple preparation method as well as simplicity and convenience for operation. By adoption of the semi-artificial feed and the feeding method provided by the invention, a large amount of anoplophora chinensis larvae for tests can be provided for scientific researches.

Description

Meridic diet of a kind of longicorn beetle larva and rearing method
Technical field
The present invention relates to a kind of meridic diet and rearing method of insect larvae, particularly relate to meridic diet and the method for breeding thereof of longicorn beetle larva.
Technical background
Longicorn beetle Anoplophora chinensis, be commonly called as white star longicorn, silver-colored longicorn beetle, belong to coleoptera (Coleoptera) Cerambycidae (Cerambycidae), ditch shin longicorn subfamily (Lamiinae) longicorn beetle genus ( AnoplophoraHope).The host of longicorn beetle is very extensive, can endanger 19 sections 29 and belong to 48 kinds.Main host plants: horse-tail, poplar, willow, elm, locust tree, walnut, mulberry tree, toon, Chinese catalpa, Chinese tallow tree, Chinese parasol tree, acacia rachii, China tree, plane tree, female life, oak, citrus and other woods fruit plant.With the trunk or the main root of larva moth food near-earth, destroy the transportation of setting recuperate branch and moisture, so that tree vigo(u)r is weak, the whole strain of weight person is withered.
In China southeastern coastal areas, longicorn beetle is to the coast protection forest vanguard tree seed---and horse-tail has been caused serious harm, and according to investigations, in some horse-tail standing forests, hazard ratio is up to more than 50%.Longicorn beetle newly hatched larvae moth food bast internal layer and form layer around the tocostome under bark, after pierce xylem harm.The lighter influences growth, and weight person subjects to windbreak, and causes other disease and pest to take place, and causes forest dead, has a strong impact on the construction of shelter-forest project, threatens local people to live and work in peace and contentment.
On China oranges and tangerines producing region and forestry, green tree species, longicorn beetle harm is also very general and serious, has brought tremendous loss to production of forestry.
Longicorn beetle larva life is hidden, in trunk life cycle long, make its control very difficult.Biological control is one of effective means of longicorn beetle Comprehensive Control, as using muscardine in the roundheaded borer phase Beauveria bassiana, green muscardine fungus Metarhizium anisopliaeBiologic products such as sticking cream have good prevention effect, or the swollen leg honeybee of Shi Fangguanshi Scleroderma guani, Scleroderma sichuanensis Xiao Scleroderma sichuanensisDeng parasitic wasp natural preferably control action is arranged also.But raise owing to can't carry out roundheaded borer, can't provide a large amount of roundheaded borer examination worms to carry out indoor pharmacodynamic test or pathogenicity mensuration, thereby can't screen and produce the high-performance bio pesticide that can be applicable to prevent and treat roundheaded borer.Simultaneously, owing to be difficult to solve the vector problem of natural enemy insect, also can't breed natural enemy insect in a large number.
A generation took place in general 1 year in longicorn beetle, and larval phase long (more than 300 days), the artificial feeding difficulty is very big, did not have any report about the artificial feeding of longicorn beetle larva at present.Other only a few longicorn beetle larva artificial feeding, the complicated component of its man-made feeds, raw material are difficult for obtaining, and cost is high.
To the problems referred to above; Carry out artificial feeding longicorn beetle larva; Both can conveniently observe the longicorn beetle life habit; Also can also carrier be provided for breeding longicorn beetle natural enemy insect, and technical support be provided for scientific researches such as biologicall test or toxicity test provide required test material to the protein food development and utilization of roundheaded borer.
For these reasons, we have carried out the invention research of longicorn beetle larva meridic diet and method for breeding thereof.
Summary of the invention
The purpose of this invention is to provide meridic diet of a kind of longicorn beetle larva and preparation method thereof, the present invention also aims to provide the rearing method of a kind of longicorn beetle larva.Meridic diet comprehensive nutrition of the present invention, cost is low and preparation method is simple, and is easy and simple to handle.Through the longicorn beetle larva that meridic diet of the present invention and method for breeding are raised, can be scientific research a large amount of test examination worms are provided.
Technical scheme of the present invention:
A kind of longicorn beetle larva meridic diet, the parts by weight of each component are in the said meridic diet: 50 ~ 60 parts in sawdust, 25 ~ 30 parts of analysis for soybean powder; 25 ~ 30 parts in wheat bran, 2～3 parts of dusty yeasts, 2～3 parts of casein; 0.5 ~ 1 part of food preservative, 5 ~ 10 parts in agar, 130～170 parts in water.
Said sawdust is the sawdust of longicorn beetle host plant, and said host plant comprises horse-tail, China tree, oranges and tangerines, willow or birch, and sawdust weight is the dry weight after drying.
Described food preservative is sorbic acid, potassium sorbate or Sodium Benzoate.
The preparation method of described longicorn beetle larva meridic diet comprises mixing, stirring, the sterilization of raw material, it is characterized in that:
Figure 2011101052137100002DEST_PATH_IMAGE002
is with 50 ~ 60 parts of sawdusts; 25 ~ 30 parts of analysis for soybean powder and 25 ~ 30 parts of wheat bran mix; Fully mix thoroughly, subsequent use;
Figure DEST_PATH_IMAGE004
is with 2～3 parts of dusty yeasts; 2～3 parts of casein and 0.5 ~ 1 part of mixing of food preservative; Fully mix thoroughly, subsequent use;
Figure DEST_PATH_IMAGE006
uses a clean container; Fetch water 130～170 parts; To wherein adding 5 ~ 10 parts in agar; Heating is also constantly stirred; Boil; After treating that agar dissolves fully, slowly add the mixture of step
Figure 724529DEST_PATH_IMAGE004
and step
Figure 579353DEST_PATH_IMAGE002
successively, stir while feeding in raw material; In order to avoid produce caking, make the longicorn beetle larvae feeds;
Figure DEST_PATH_IMAGE008
packs the longicorn beetle larvae feeds branch that stirs in the container; With the raw glass rod with the feed compacting; Feed is filled to 1/2 to 2/3 of container volume, cover lid;
The container that
Figure DEST_PATH_IMAGE010
will be equipped with the longicorn beetle larvae feeds is positioned in the autoclave; The 30min that under 121 ℃ of conditions, sterilizes promptly obtains longicorn beetle larva meridic diet after the cooling.
The described container of step
Figure 483724DEST_PATH_IMAGE008
comprises 140ml container or 300ml container; Wherein the 140ml container is the test tube (length 200mm, bore 30mm) of PC material with cover, and the 330ml container is the tissue culture bottle (high 107mm, bore 60mm, footpath, end 70mm) of the PC material of band gas permeable devices.
A duck eye digs with aseptic instrument in longicorn beetle larva meridic diet central authorities making, is convenient to the longicorn beetle larva and bores moth entering feed inside, and the size in hole is decided according to the polypide size.
The present invention also provides the rearing method of a kind of longicorn beetle larva, comprises collection and the raising of ovum, larva, and concrete steps are following:
Figure 878933DEST_PATH_IMAGE002
collects ovum: catch the longicorn beetle adult in woodland; Place dependent insect cage; With longicorn beetle host plant spray feeding; Place longicorn beetle host plant juggle simultaneously; Supply the longicorn beetle adult to lay eggs, and will produce in the juggle insertion water of ovum and preserve moisture, treat that the longicorn beetle ovum hatches naturally; Or after the longicorn beetle adult has produced ovum, directly strip out the ovum in the juggle, preserve moisture, indoor hatching; Saidly preserve moisture, the method for indoor hatching is: ovum is put down gently on the wetting filter paper of sterilized water, is placed sterile petri dish together, be positioned over hatching under the dark surrounds;
larva collects and raises: the longicorn beetle small grub that careful taking-up has been hatched; And it is put down gently to off-the-shelf longicorn beetle larva meridic diet; Cover lid; Place then under the dark condition and raise; According to the growth progress of longicorn beetle, change longicorn feed and suitable raising container in good time;
the routine observation longicorn beetle larval growth situation and situation of pupating; After treating that longicorn beetle pupates, sprouts wings; Raise adult with longicorn beetle host plant spray; Let it carry out mating, lay eggs, carry out the raising of the second generation.
Said longicorn beetle host plant wood comprises Chinese ephedra, China tree, birch or willow.
Positive beneficial effect of the present invention:
meridic diet comprehensive nutrition of the present invention; Raw material sources extensively are easy to get; Cheap; Cost is low, and economic benefit is obvious.
meridic diet preparation method of the present invention is simple; Easy and simple to handle, need not special installation.
Figure 953376DEST_PATH_IMAGE006
meridic diet of the present invention can be raised multiple roundheaded borer; Like Monochamus alternatus larva, anoplophora glabripennis larva, stem borer larva etc.; The corresponding host plant sawdust that only needs the sawdust composition to be changed into certain longicorn gets final product, and method for breeding is consistent.
The longicorn beetle larva that
Figure 884422DEST_PATH_IMAGE008
raises through meridic diet of the present invention and method for breeding; Can be scientific research a large amount of tests examination worms are provided, also can be and breed natural enemy insect vector etc. is provided.
Figure 638752DEST_PATH_IMAGE010
raises the longicorn beetle larva through method for breeding of the present invention, and the raising cost of container is cheap, and volume is little; It is little to take up space; Can reuse, be convenient to observe its history of life simultaneously, raise and need not special installation; The raising survival rate is high, and feeding effect is stable.
The specific embodiment
Embodiment one:
1. longicorn beetle larva meridic diet is filled a prescription:
Horse-tail sawdust 200g, analysis for soybean powder 100g, wheat bran 100g, dusty yeast 10g, casein 10g, sorbic acid 4g, agar 40g, water 600ml.
2. meridic diet preparation method:
Figure 562714DEST_PATH_IMAGE002
takes by weighing 200g horse-tail sawdust; The 100g analysis for soybean powder; 100g wheat bran; Be positioned in the plastic tub, fully mix thoroughly, subsequent use;
takes by weighing dusty yeast 10g; Casein 10g; Sorbic acid 4g; Be positioned in the beaker, fully mix thoroughly, subsequent use;
Figure 453627DEST_PATH_IMAGE006
is with the stainless-steel pan 600ml that fetches water; To wherein adding agar 40g; Heating is also constantly stirred; Boil; After treating that agar dissolves fully; Slowly add step
Figure 882203DEST_PATH_IMAGE004
and the described mixture of step
Figure 95010DEST_PATH_IMAGE002
successively; Stir while feeding in raw material, in order to avoid produce caking;
is with the larvae feeds branch that stirs 140ml Boiling tube (the length 200mm that packs into; Bore 30mm) in; With the mortar rod with the feed compacting; Feed is filled to 2/3 of container volume, cover lid;
Figure 260598DEST_PATH_IMAGE010
is positioned over the longicorn beetle larvae feeds for preparing in the autoclave; The 30min that under 121 ℃ of conditions, sterilizes, the cooling back is subsequent use;
Figure DEST_PATH_IMAGE012
digs a duck eye in feed central authorities with glass bar, so that roundheaded borer bores moth.
3. longicorn beetle larva raising method:
⑴ collect larva: catch the longicorn beetle adult in woodland, place dependent insect cage, with horse-tail or China tree spray feeding; Placing diameter simultaneously is the China tree juggle about 5cm; Supply longicorn to lay eggs, and will produce in the juggle insertion water of ovum and preserve moisture, let the longicorn beetle ovum hatch naturally.
⑵ after treat that larva hatches 5-7 days; Carefully with the bark of above-mentioned juggle with pocket knife strip off gently, the longicorn small grub that careful taking-up has been hatched, and it is put down gently to off-the-shelf meridic diet; Place under the dark condition and raise; According to the growth progress of longicorn, change the longicorn feed, weighing larva polypide weight when at every turn changing feed in good time.
When
Figure 927203DEST_PATH_IMAGE006
requires when larval growth to Pass Test examination worm, can be used for scientific researches such as biologicall test or toxicity test.
4. feeding effect: raise 300 of longicorn beetle larvas through above-mentioned method for breeding.Behind the 30d, average every net gain 0.663g, 4.64 times of average weight gains; Average every larva net gain 1.371g behind the 100d is 9.90 times of initial larva; The 30d survival rate of larvae is 95.7%, is 92.0% during 100d, visible longicorn beetle larva well-grown in meridic diet of the present invention, and survival rate is high.The roundheaded borer of raising out can be scientific research provides physiological standard unified examination worm, also can be used as the vector of breeding natural enemy insect.
Table 1 longicorn beetle feeding effect
Embodiment two:
1. longicorn beetle larva meridic diet is filled a prescription:
Horse-tail sawdust 200g, analysis for soybean powder 100g, wheat bran 100g, dusty yeast 10g, casein 10g, potassium sorbate 4g, agar 20g, water 600ml.
2. meridic diet preparation method: (with embodiment one)
3. longicorn beetle larva raising method:
⑴ collect larva: catch the longicorn beetle adult in woodland, place dependent insect cage, with horse-tail spray feeding, placing diameter simultaneously is the China tree juggle about 5cm, supplies longicorn to lay eggs, and will produce in the juggle insertion water of ovum and preserve moisture.
⑵ directly strip out the ovum in the juggle after the longicorn adult has produced ovum, carry out artificial incubation.Artificial incubation method: ovum is placed on the wetting filter paper of sterilized water gently; Place sterile petri dish together; Be positioned under the dark surrounds, can hatch about 10d and it is put down gently to off-the-shelf meridic diet, place then under the dark condition and raise; According to the growth progress of longicorn, change longicorn feed and suitable raising container in good time.
4. routine observation longicorn beetle history of life, behind the adult mate and oviposit of emergence, the larva that hatches is used for the second generation raising of going down to posterity.

Claims (7)

1. the meridic diet of a longicorn beetle larva, it is characterized in that: the parts by weight of each component are in the said meridic diet: 50～60 parts in sawdust, 25～30 parts of analysis for soybean powder; 25～30 parts in wheat bran; 2～3 parts of dusty yeasts, 2～3 parts of casein, 0.5～1 part of food preservative; 5～10 parts in agar, 130～170 parts in water;
Said sawdust is the sawdust of longicorn beetle host plant, and said host plant comprises horse-tail, China tree, oranges and tangerines, willow or birch, and sawdust weight is the dry weight after drying.
2. the meridic diet of longicorn beetle larva according to claim 1 is characterized in that: described food preservative is sorbic acid, potassium sorbate or Sodium Benzoate.
3. the preparation method of a longicorn beetle larva meridic diet according to claim 1 or claim 2 comprises mixing, stirring, the sterilization of raw material, it is characterized in that:
Figure 521355DEST_PATH_IMAGE001
is with 50～60 parts of sawdusts; 25～30 parts of analysis for soybean powder and 25～30 parts of wheat bran mix; Fully mix thoroughly, subsequent use;
is with 2～3 parts of dusty yeasts; 2～3 parts of casein and 0.5～1 part of mixing of food preservative; Fully mix thoroughly, subsequent use;
Figure 266774DEST_PATH_IMAGE003
uses a clean container; Fetch water 130～170 parts; To wherein adding 5～10 parts in agar; Heating is also constantly stirred; Boil; After treating that agar dissolves fully; The mixture that slowly adds step
Figure 702435DEST_PATH_IMAGE002
and step
Figure 474082DEST_PATH_IMAGE001
successively; Stir while feeding in raw material,, make the longicorn beetle larvae feeds in order to avoid produce caking;
Figure 362403DEST_PATH_IMAGE004
packs the longicorn beetle larvae feeds branch that stirs in the container; With the raw glass rod with the feed compacting; Feed is filled to 1/2 to 2/3 of container volume, cover lid;
The container that
Figure 244909DEST_PATH_IMAGE005
will be equipped with the longicorn beetle larvae feeds is positioned in the autoclave; The 30min that under 121 ℃ of conditions, sterilizes promptly obtains longicorn beetle larva meridic diet after the cooling.
4. the preparation method of longicorn beetle larva meridic diet according to claim 3 is characterized in that: the described container of step
Figure 535076DEST_PATH_IMAGE004
comprises 140ml container or 300ml container; Wherein the 140ml container is the test tube of PC material with cover, and the 330ml container is the tissue culture bottle of the PC material of band gas permeable devices.
5. the preparation method of longicorn beetle larva meridic diet according to claim 3; It is characterized in that: a duck eye digs with aseptic instrument in the longicorn beetle larva meridic diet central authorities making; Be convenient to the longicorn beetle larva and bore moth entering feed inside, the size in hole is decided according to the polypide size.
6. the rearing method of a longicorn beetle larva comprises collection and the raising of ovum, larva, and it is characterized in that: concrete steps are following:
Figure 412377DEST_PATH_IMAGE001
collects ovum: catch the longicorn beetle adult in woodland; Place dependent insect cage; With longicorn beetle host plant spray feeding; Place longicorn beetle host plant juggle simultaneously; Supply the longicorn beetle adult to lay eggs, and will produce in the juggle insertion water of ovum and preserve moisture, treat that the longicorn beetle ovum hatches naturally; Or after the longicorn beetle adult has produced ovum, directly strip out the ovum in the juggle, preserve moisture, indoor hatching; Saidly preserve moisture, the method for indoor hatching is: ovum is put down gently on the wetting filter paper of sterilized water, is placed sterile petri dish together, be positioned over hatching under the dark surrounds;
Figure 850312DEST_PATH_IMAGE002
larva collects and raises: the longicorn beetle small grub that careful taking-up has been hatched; And it is put down gently to off-the-shelf longicorn beetle larva meridic diet; Cover lid; Place then under the dark condition and raise; According to the growth progress of longicorn beetle, change longicorn feed and suitable raising container in good time; The parts by weight of each component are in the said longicorn beetle larva meridic diet: 50～60 parts in sawdust, 25～30 parts of analysis for soybean powder, 25～30 parts in wheat bran, 2～3 parts of dusty yeasts, 2～3 parts of casein, 0.5～1 part of food preservative, 5～10 parts in agar, 130～170 parts in water; Said sawdust is the sawdust of longicorn beetle host plant, and said host plant comprises horse-tail, China tree, oranges and tangerines, willow or birch, and sawdust weight is the dry weight after drying;
Figure 208612DEST_PATH_IMAGE003
the routine observation longicorn beetle larval growth situation and situation of pupating; After treating that longicorn beetle pupates, sprouts wings; Raise adult with longicorn beetle host plant spray; Let it carry out mating, lay eggs, carry out the raising of the second generation.
7. the rearing method of longicorn beetle larva according to claim 6 is characterized in that: said longicorn beetle host plant comprises horse-tail, China tree, oranges and tangerines, birch or willow.
?
?
?
CN2011101052137A 2011-04-26 2011-04-26 Semi-artificial feed for anoplophora chinensis larvae and artificial feeding method Expired - Fee Related CN102217729B (en)

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