CN107736307A - A kind of method that fungus gnat is raised using sweet potato - Google Patents
A kind of method that fungus gnat is raised using sweet potato Download PDFInfo
- Publication number
- CN107736307A CN107736307A CN201710998444.2A CN201710998444A CN107736307A CN 107736307 A CN107736307 A CN 107736307A CN 201710998444 A CN201710998444 A CN 201710998444A CN 107736307 A CN107736307 A CN 107736307A
- Authority
- CN
- China
- Prior art keywords
- sweet potato
- fungus gnat
- bradysia
- vermiculite
- insect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000017020 Ipomoea batatas Species 0.000 title claims abstract description 97
- 235000002678 Ipomoea batatas Nutrition 0.000 title claims abstract description 96
- 241001157791 Mycetophilus sp. HMR-1993 Species 0.000 title claims abstract description 85
- 238000000034 method Methods 0.000 title claims abstract description 67
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 51
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 50
- 239000010455 vermiculite Substances 0.000 claims abstract description 50
- 241000238631 Hexapoda Species 0.000 claims abstract description 42
- 241001494113 Bradysia Species 0.000 claims description 42
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 33
- 241000613693 Bradysia odoriphaga Species 0.000 claims description 23
- 229920003023 plastic Polymers 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 244000061456 Solanum tuberosum Species 0.000 claims description 14
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 14
- 241001635185 Sciara Species 0.000 claims description 13
- 210000002615 epidermis Anatomy 0.000 claims description 12
- 241000220617 Megaselia nigra Species 0.000 claims description 11
- 241000256095 Sciaridae Species 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 241000681686 Lycoriella Species 0.000 claims description 8
- 241000372295 Bradysia impatiens Species 0.000 claims description 7
- 241000328772 Lycoriella ingenua Species 0.000 claims description 6
- 241001157808 Mycetophilidae Species 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 241000545744 Hirudinea Species 0.000 claims description 5
- 239000004575 stone Substances 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 22
- 230000001488 breeding effect Effects 0.000 abstract description 22
- 239000002994 raw material Substances 0.000 abstract description 12
- 230000004083 survival effect Effects 0.000 abstract description 10
- 238000012360 testing method Methods 0.000 abstract description 10
- 230000032669 eclosion Effects 0.000 abstract description 9
- 230000017448 oviposition Effects 0.000 abstract description 9
- 230000019617 pupation Effects 0.000 abstract description 9
- 239000011159 matrix material Substances 0.000 abstract description 7
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 230000035699 permeability Effects 0.000 abstract description 3
- 230000001850 reproductive effect Effects 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 235000005254 Allium ampeloprasum Nutrition 0.000 description 23
- 240000006108 Allium ampeloprasum Species 0.000 description 23
- 239000004033 plastic Substances 0.000 description 14
- 241000255925 Diptera Species 0.000 description 10
- 241000233866 Fungi Species 0.000 description 9
- 239000000463 material Substances 0.000 description 8
- 235000018645 Allium odorum Nutrition 0.000 description 7
- 235000017060 Arachis glabrata Nutrition 0.000 description 7
- 244000105624 Arachis hypogaea Species 0.000 description 7
- 235000010777 Arachis hypogaea Nutrition 0.000 description 7
- 235000018262 Arachis monticola Nutrition 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 7
- 241000607479 Yersinia pestis Species 0.000 description 7
- 235000020232 peanut Nutrition 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 230000002265 prevention Effects 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 240000008654 Allium ramosum Species 0.000 description 4
- 244000052909 Dioscorea esculenta Species 0.000 description 4
- 239000003905 agrochemical Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 239000004576 sand Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 244000291564 Allium cepa Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 235000002722 Dioscorea batatas Nutrition 0.000 description 3
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 3
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 2
- 244000000383 Allium odorum Species 0.000 description 2
- 240000002234 Allium sativum Species 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 241000382353 Pupa Species 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 235000004611 garlic Nutrition 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000000123 paper Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000222532 Agrocybe Species 0.000 description 1
- 235000010167 Allium cepa var aggregatum Nutrition 0.000 description 1
- 244000003377 Allium tuberosum Species 0.000 description 1
- 235000005338 Allium tuberosum Nutrition 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241000208173 Apiaceae Species 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 235000002723 Dioscorea alata Nutrition 0.000 description 1
- 235000008532 Dioscorea bulbifera Nutrition 0.000 description 1
- 235000007056 Dioscorea composita Nutrition 0.000 description 1
- 235000009723 Dioscorea convolvulacea Nutrition 0.000 description 1
- 235000005362 Dioscorea floribunda Nutrition 0.000 description 1
- 235000004868 Dioscorea macrostachya Nutrition 0.000 description 1
- 235000005361 Dioscorea nummularia Nutrition 0.000 description 1
- 235000005360 Dioscorea spiculiflora Nutrition 0.000 description 1
- 241000222684 Grifola Species 0.000 description 1
- 235000006350 Ipomoea batatas var. batatas Nutrition 0.000 description 1
- 208000000291 Nematode infections Diseases 0.000 description 1
- 241001560086 Pachyrhizus Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241000222351 Pleurotus cornucopiae Species 0.000 description 1
- 244000252132 Pleurotus eryngii Species 0.000 description 1
- 235000001681 Pleurotus eryngii Nutrition 0.000 description 1
- 241000318836 Pleurotus nebrodensis Species 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241001074085 Scophthalmus aquosus Species 0.000 description 1
- 108010016634 Seed Storage Proteins Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 231100000570 acute poisoning Toxicity 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 244000010262 apichu Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 235000004879 dioscorea Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000035929 gnawing Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000011232 storage material Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Food Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of method that fungus gnat is raised using sweet potato, belong to the artificial feeding field of insect.The inventive method first spreads vermiculite in container, sweet potato then is placed above in vermiculite, then cover last layer vermiculite；Container is placed in insect cage, the female male imago of fungus gnat is proportionally accessed, is finally raised in biochemical cultivation case.Nutrient sweet potato in the inventive method as feed enriches, and can meet that fungus gnat respectively grows needs from generation to generation, improves reproductive efficiency and quality；And by the use of the strong vermiculite of good water-retaining property, gas permeability as matrix, good environmental condition is provided for fungus gnat breeding.The survival rate of fungus gnat, percentage of pupation, eclosion rate, single female egg laying amount are good apparently higher than prior art, reproductive effect in the inventive method；And the uniformity and stability of gained larva are good, the confidence level and reliability of biologic test are accordingly improved, in addition, the present invention is adapted for large-scale breeding.Raw material of the present invention is easy to get, method is simple, raw material and cost of labor are low.
Description
Technical field
The invention belongs to the rearing method of insect, and in particular to a kind of method that fungus gnat is raised using sweet potato.
Background technology
Fungus gnat is the system of Diptera eye Sciaridae (Mycetophilidae) and Lycoriidae (Sciaridae) insect
Claim.Bradysia Sciara (Bradysia) and strict eye Sciara (Lycoriella) insect wherein in eye Sciaridae is to crops
Harm is the most serious, including belongs to the Bradysia odoriphaga (Bradysia odoriphaga) of Bradysia Sciara, different Bradysia fungus gnat
(Bradysia difformis) and mushroom fly (Bradysia minpleuroti), and belong to the flat mushroom of strict eye Sciara
Strict eye fungus gnat (Lycoriella pleuroti) etc..
Bradysia odoriphaga (Bradysia odoriphaga), is commonly called as fragrant-flowered garlic maggot, is the main original for causing the leek underproduction at present
Cause.The worm is distributed widely in the ground such as China northeast, North China, Central China and northwest, is the distinctive insect in China.Fragrant-flowered garlic maggot host range
Extensively, except endangering leek, garlic, shallot and onion, the section of composite family, Chenopodiaceae, Cruciferae, Curcurbitaceae and Umbelliferae etc. 6 is also endangered
30 various vegetables.Fragrant-flowered garlic maggot is gregarious in leek bulb position mainly by larva and carries out gnawing harm, causes plant withered, even dead
Die, have a strong impact on the yield and quality of leek.Fragrant-flowered garlic maggot is the most serious to the harm of greenhouse leek, and the underproduction is up to 30%~80%.Fragrant-flowered garlic
Maggot individual is small, occur and harm is disguised strong, and difficulty of prevention and cure is big, it has also become a great problem in leek production.
With the popularization of China's fungus growing technique, cultivated area constantly expands, place recycling, Edible Fungi Pests
Harm also rising year by year, different Bradysia fungus gnat (Bradysia difformis), mushroom fly wherein in eye Sciaridae
The insect such as (Bradysia minpleuroti) and the strict eye fungus gnat of flat mushroom (Lycoriella pleuroti) be cultivation and it is wild
Edible mushroom, the sociales of medicinal fungus insect, and one of important restriction factor as Edible Fungi Industry Development.The food that they endanger
Mainly have with strain class：Flat mushroom, Agricus blazei, agrocybe, pleurotus eryngii, Pleurotus nebrodensis, asparagus, pleurotus cornucopiae, grifola frondosus and black fungus etc..
Such adult pest is called mushroom mosquito by mushroom grower, and larva is referred to as mushroom maggot.The harm of mushroom mosquito is mainly taken by mushroom maggot brill moth into bacterium material
Mycelium and organic matter are eaten, mycelium can be eaten up when serious, compost is eaten into poroid；Mushroom mosquito is also mushroom class disease, line simultaneously
Worm, the disseminator of mite class, the development duration of the worm is short in addition, and mushroom mosquito can set up kind in a short time once mushroom house is flown into
Group.Therefore, occur in mushroom mosquito in serious mushroom house, the probability that bacterium bag is endangered by miscellaneous bacteria and nematode infections is also higher.
The main method of preventing and treating fungus gnat is chemical prevention at present, and most of vegetable grower is entered using the method for agricultural chemicals pouring root and spraying
Row preventing and treating, but leek and fruit body of edible fungi growth cycle it is short, directly eat, residues of pesticides to the toxic effect of human body, and
Leek is permanent vegetable, and the long-term of chemical pesticide is largely used so that chemical agent is enriched with leek, is absorbed from soil
After leek after agricultural chemicals is eaten by consumer, acute poisoning event is happened occasionally, and great threat is caused to the health of people.Therefore,
Need the prevention and controls beyond searching chemical prevention badly.
Need substantial amounts of larva to use worm as experiment in fungus gnat study on prevention, thus the indoor mass rearing of fungus gnat be into
The basis of the related study on prevention of row.Mainly using former host plant or hypha,hyphae as feed in conventional method for breeding, such as
Using leek section as feed in the artificial feeding of traditional Bradysia odoriphaga, that is, leek is gathered, go the removal of impurity, peeling, by leek
4~6mm segments are cut into, are eaten for olaiphage larva in Chinese Chive, using natural leek as feed in whole feeding process,
Subject matter existing for this method for breeding is：It can not be completely removed with certain residues of pesticides in leek, this band
Murder by poisoning of the medicine leek to fungus gnat is larger, causes the discontinuous of raising so that the raising of more generations of fungus gnat can not be carried out, and be also resulted in for examination
The uniformity of fungus gnat is poor, reduces the confidence level and representativeness of result of the test；Secondly, the Bradysia odoriphaga obtained by the method is quick
Perception is poor, causes result of the test inaccurate；In addition, leek is not easy to maintain in itself, it is perishable mouldy, it is necessary to often change fresh fragrant-flowered garlic
Dish and vessel, make the very cumbersome trouble of feeding process, improve feeding cost, and be difficult to be raised on a large scale.
In addition to by the use of leek as feed, the feed for raising Bradysia odoriphaga also has man-made feeds (such as
CN101427666A), mushroom powder, dusty yeast, preservative and agar powder used wherein in man-made feeds etc. not only increase feeding
Expect cost, also to prevent the rotten requirement improved to vessel and environment of man-made feeds, often more new feedstuff and vessel, operation
It is cumbersome, manpower and materials input amount is big, feeding cost is high, and is extremely difficult to extensive rearing conditions.It is in addition, slow for raising leek
The feed of eye fungus gnat also has peanut (CN104542498A) or potato (CN104509494A) etc., and feed is used as using peanut or potato
Method for breeding in not only peanut used, potato it is expensive, and peanut early stage, potato decortication are scalded with boiling water, and fine sand
Acquisition etc. be required for a large amount of human and material resources to put into, in addition, be put into fine sand in vessel, it is heavy to also result in vessel, is not easy to carry,
This method operation is relatively complicated, and wastes time and energy, and cost is high, it is also difficult to is raised on a large scale.
In addition to the selection of feed, another importance of raising eye fungus gnat is the requirement to feeding environment humidity.
It is main at present to use the humidity that feeding environment is kept in a manner of filter paper, agar, absorbent cotton or fine sand etc. add water for matrix, still
A common issue existing for these matrix is that moisture preserving time is short, it is necessary to constantly add water.
Sweet potato belongs to Convolvulaceae sweet potato genus (Ipomoea batatas (L.)), also known as sweet potato, sweet potato, sweet potato, sweet potato,
Red Hill medicine, Yuzhen potato, sweet potato, pachyrhizus, Chinese yam or yam etc., because having different titles from different places.Sweet potato is important grain
Food, feed, the raw material of industry and novel energy use crop.As potato wedge can be as the raw material of staple food, wine or beverage, fresh sweet potato and cauline leaf can
As feed etc..
Vermiculite is a kind of natural, nontoxic mineral matter, can expand to form expanded vermiculite under high temperature action, is had powerful
Water-retaining property has gas permeability concurrently.At present, vermiculite is mainly used for golf course lawn, Seed storage agent, soil in agricultural field
Conditioning agent, wetting agent, plant production agent or feed addictive etc..
Understand that sweet potato is not the host of fungus gnat, does not find to be used as feed artificial propagation insect side by the use of sweet potato through retrieval
The report in face；Also without the report found in terms of vermiculite is used for into insect reproduction.
The content of the invention
The host of fungus gnat is mainly green onion, the liliaceous plant of garlic class, and edible mushroom etc., but the present inventor was studying
Had been surprisingly found that in journey, by the use of sweet potato as forage feed fungus gnat, more preferable effect can be obtained.The present invention is exactly to have been surprisingly found that herein
On the basis of formed.
Present invention aims at provide application of the sweet potato as feed in fungus gnat artificial feeding.
Another object of the present invention is to provide a kind of method for raising fungus gnat using sweet potato.
To achieve the above object, technical scheme is as follows：
The invention provides application of the sweet potato as feed in fungus gnat artificial feeding.
Fungus gnat described in above-mentioned application refers to the Bradysia Sciara of a Sciaridae (Mycetophilidae)
Or strict eye Sciara (Lycoriella) insect (Bradysia).
Described Bradysia Sciara insect refers to Bradysia odoriphaga (Bradysia odoriphaga), different Bradysia fungus gnat
(Bradysia difformis), mushroom fly (Bradysia minpleuroti), Fujian mushroom Bradysia fungus gnat (Bradysia
) or Lu Shi Bradysias fungus gnat (Bradysia luhi) etc. minpleuroti.
Described strict eye Sciara insect refers to the strict eye fungus gnat of flat mushroom (Lycoriella pleuroti) or the strict eye gill fungus of cloud mushroom
Mosquito (Lycoriella yunpleuroti) etc..
Present invention also offers a kind of method that fungus gnat is raised using sweet potato, comprise the following steps：
(1) pre-process：The sweet potato epidermis of fresh and healthy is cleaned up, then naturally dry to epidermis is dried, then in purple
2~4h is irradiated under outer lamp；
(2) from the clean container that height is 15~20cm, the vermiculite that thickness is 5~7cm, Ran Hou are spread in container
1~3 piece of the sweet potato of step (1) pretreatment is placed above in vermiculite, then is covered with the vermiculite that a layer thickness is 3~5cm, exposed in vermiculite
Sweet potato above layer is highly 2~4cm；Container is placed in insect cage；
(3) the male and female adult population of same fungus gnat is accessed in insect cage, then the supporting equipped with fungus gnat adult by step
Worm cage is placed in biochemical cultivation case again, is 24~26 DEG C in temperature, illumination 16L：Raised under the conditions of 8D；
(4) treat that sweet potato pulp will be by the complete space-time of feeding one, in Adult worms producting eggs peak period, by equipped with fresh sweet potato and vermiculite
Container is put into insect cage, replaces the potato wedge totally by feeding, is repeated above step and is continued to raise.
The size of sweet potato described in above-mentioned method for breeding step (1) is 200~800g/.
Cleaning described in above-mentioned method for breeding step (1) refers to be cleaned with water.
Container described in above-mentioned method for breeding step (2) is transparent plastic casing, or is transparent plastics basin.It is described
Plastic casing or plastics basin etc. using preceding carrying out disinfection.
The particle size of vermiculite described in above-mentioned method for breeding step (2) is 1~3mm.
The water content of vermiculite described in above-mentioned method for breeding step (2) is 70~80%.Hold agglomerating, but can not drip
Water comes, and hand pine group does not scatter entirely.
Insect cage described in above-mentioned method for breeding step (2) is shaped as square.Insect cage can be bought in the market.
The specification of above-mentioned insect cage can determine according to raising scale, as specification is long 350mm, wide 350mm, high 350mm
200~250 female male imagos are can access in insect cage.
The structure composition of insect cage described in above-mentioned method for breeding step (2) includes inside casing, and yarn is enclosed with outside inside casing
Net.The material of described inside casing is plastic frame.The size of described plastic frame is corresponding to being wrapped in outer gauze size.
Described gauze is 120 mesh.The material of the gauze can be nylon wire.
The ratio of female male imago described in above-mentioned method for breeding step (3) is 1~2：1.
The quantity of the female male imago of access described in above-mentioned method for breeding step (3) can determine according to the size of insect cage,
Such as exist
Fungus gnat described in above-mentioned method for breeding step (3) refers to a Sciaridae (Mycetophilidae) insect.
Described eye Sciaridae insect refers to Bradysia Sciara (Bradysia) or strict eye Sciara (Lycoriella) elder brother
Worm.
Described Bradysia Sciara insect refers to Bradysia odoriphaga (Bradysia odoriphaga), different Bradysia fungus gnat
(Bradysia difformis) or mushroom fly (Bradysia minpleuroti) etc..
Described strict eye Sciara insect refers to the strict eye fungus gnat of flat mushroom (Lycoriella pleuroti) or the strict eye gill fungus of cloud mushroom
Mosquito (Lycoriella yunpleuroti) etc..
Temperature described in above-mentioned method for breeding step (3) is preferably 26 DEG C.
Calculated in above-mentioned method for breeding according to 200~250 fungus gnats of access, the generation of fungus gnat 2~3 can be bred per 500g sweet potatoes.
2~4 days ovum phases of usual fungus gnat, 10~12 days larval phases；2~3 days pupa time, 3~5 days adult stages；20~25 days
Complete a generation.Larva can be taken at any time in larval phase and carry out correlation test.
Compared with prior art, the present invention has the advantage that and beneficial effect：(1) effect of the inventive method breeding fungus gnat
It is good, using the survival rate of fungus gnat obtained by the inventive method, percentage of pupation, eclosion rate, single female egg laying amount apparently higher than prior art；Its
Secondary, the uniformity and stability of fungus gnat obtained by the inventive method greatly improve, correspondingly improve biologic test confidence level and
Reliability.(2) sweet potato is rich in protein and various amino acid in the inventive method, nutritious, can meet fungus gnat larva
Needed for growing, it is also ensured that the sustainable supply of nutrition when adult mates, you can to meet that fungus gnat respectively grows from generation to generation
Nutritional need, improve fungus gnat breeding efficiency and quality.(3) sweet potato in the inventive method as fungus gnat feed is easy to four
Ji Baocun, material are easy to get, and compared with leek, peanut, potato and Chinese yam, sweet potato price is low.(4) in the inventive method except
Clean surface, sweet potato need not other processing, and the fresh leek of existing fungus gnat feed, peanut, potato, Chinese yam etc. be required for into
Row processing and continuous renewal, not only reduce effect, and improve processing cost.(5) the inventive method with sweet potato as feeding
Material, low is required to environmental condition；And with leek, peanut, potato etc. as feed, because natural leek is perishable, needs often more
Change feed, keep humidity etc., thus, labor intensive high to environmental requirement, and reproductive effect is poor.(6) sweet potato does not have agricultural chemicals
Residual or residual are very low, fungus gnat are not poisoned or poisoned very little, ensure that the continuity of raising, and then ensure that for examination gill fungus
The uniformity of mosquito, enhance the confidence level and reliability of result of the test.Further, since sweet potato further avoids by pretreatment
For the Pesticide Residue of examination fungus gnat, the safety in feeding process is so ensured that, breeding come each will not for fungus gnat
Developed immunity to drugs due to stimulation components such as agricultural chemicals, susceptibility of the fungus gnat for experiment is improved, so as to improve biologic test
Accuracy rate and authenticity.(7) the inventive method using vermiculite as matrix, with the matrix such as filter paper, agar, absorbent cotton and fine sand
Compare, vermiculite has good water-retaining property and the strong characteristic of gas permeability, is also the production of fungus gnat adult while sweet potato healthy growth is ensured
Ovum, the growing of larva, change cocoon and sprout wings and provide good place, avoid in conventional method for breeding the transfer of ovum and
Waste, improve the efficiency and quality of breeding；Secondly, vermiculite good water-retaining property, number of water is greatly reduced；In addition, vermiculite cost
Low and repeatable recycling, reduce feeding cost；In addition, vermiculite can also provide nutriment for the growth of sweet potato.(9) originally
Inventive method is adapted for raising on a large scale, and former method by feed, matrix, environmental condition etc. due to being limited, it is difficult to
Raised on a large scale.(10) the inventive method is simple, and raw material and cost of labor are low.
Embodiment
The present invention will be further explained by the following examples, but the invention is not restricted to this.
Worm is used in experiment in following examples：Bradysia odoriphaga (Bradysia odoriphaga), different Bradysia fungus gnat
(Bradysia difformis), mushroom fly (Bradysia minpleuroti) and the strict eye fungus gnat (Lycoriella of flat mushroom
Pleuroti) provided by Plant Protection Institute microbial insecticide seminar of Hebei agricultural and forest science institute.
Embodiment 1 raises Bradysia odoriphaga experiment by feed of sweet potato
Carry out as follows：
(1) feed pre-processes：It is respectively 350g and 675g fresh and healthy sweet potato (sweet potato variety by two weight with water
For：Ji potato 98) epidermis cleans up, and then naturally dry to epidermis is dried, then irradiates 2h under uviol lamp.
(2) from the clean transparent plastic box that height is 15cm, the vermiculite that thickness is 5cm is spread in plastic casing, in leech
The 2 pieces of sweet potatoes pre-processed in step (1) are placed above in stone, then cover last layer 3cm vermiculites again, exposed on vermiculite
Sweet potato be highly 2cm；Plastic casing is placed in insect cage；(insect cage is shaped as square, its specification for the insect cage
It is 350mm for length, including inside casing and the nylon wire that is wrapped in outside inside casing；Wherein inside casing is and length specification
Corresponding plastic frame, the aperture of nylon wire is 120 mesh), insect cage can be commercially available (the foster worm of following examples in the market
Cage is same as Example 1).The particle size of wherein described vermiculite is 1~3mm；The water content of the vermiculite is 70~80%, 7
~10 days plus a water.
(3) access Bradysia odoriphaga (Bradysia odoriphaga) female adult pest in the insect cage described in step (2)
Insect cage, is then placed in biochemical cultivation case by 100 and male imago 50, is 24 DEG C in temperature, illumination condition 16L：8D,
Relative humidity is raised (above-mentioned sweet potato can continuously breed 3 generation Bradysia odoriphagas) under the conditions of being 70%.
(4) treat that by the complete space-time of feeding one, foster worm soon is put into the clean container equipped with sweet potato and vermiculite for sweet potato pulp
In cage, the sweet potato totally by feeding is replaced, continues to breed Bradysia odoriphaga.
The egg laying amount that each female adult (average according to female adult sum) is total in each generation, survival number are counted under the microscope
Amount, quantity of pupating, emergence quantity etc., survival rate, percentage of pupation, eclosion rate, single female production are then calculated respectively by following calculation formula
Ovum amount (grain) (average)：
Total quantity × 100% of larva quantity/ovum of survival rate (%)=hatching；
Larva quantity × 100% of the quantity for percentage of pupation (%)=pupate/total；
Quantity × 100% of the pupa of eclosion rate (%)=emerging adult quantity/total；
Spawning total quantity × 100% in single female egg laying amount (grain)=each female adult pest all one's life.
As a result Bradysia odoriphaga is raised with sweet potato, its survival rate is 93.5%, percentage of pupation 98.5%, and eclosion rate is
99%, single female egg laying amount is 120.Understand from the above, the Bradysia odoriphaga that the present invention is raised using sweet potato as feed
Survival rate, percentage of pupation, eclosion rate and single female egg laying amount obviously higher than prior art (referring to CN101427666A,
CN104542498A and CN104509494A), it is good as the feeding effect of feed using sweet potato to illustrate.Sweet potato disclosure satisfy that as feed
The nutritional need of each growth phase of Bradysia odoriphaga.Secondly, the one of the fungus gnat larva that the present invention is raised using sweet potato as feed
Cause property and stability significantly improve, and are influenceed without residues of pesticides, improve the confidence level and reliability of biologic test；This
Outside, the present invention also to raise fungus gnat on a large scale.Among other advantages, sweet potato can preserve in the four seasons, and material is easy to get,
Price is low, and does not need stripping and slicing, boiling etc. to handle before use, and method is simple；Nutrient sweet potato enriches, and can meet that fungus gnat is each
Needed for developmental stage；Sweet potato block is big, it is not necessary to and frequent updating feed, 1-2 sweet potato can continuously raise fungus gnat 2-3 from generation to generation,
Even 3-6 generations；In addition, low to being raised requirement to environment by feed of sweet potato, plus using vermiculite as matrix, water-retaining property
It is good, it is not necessary to frequently plus water, and repeatable to utilize；The inventive method raw material is cheap, and method is simple, and cost of labor is low.
Embodiment 2 is raised different Bradysia fungus gnat as feed using sweet potato and tested
Carry out as follows：
(1) pre-process：By three weight it is disease-free sweet potato (the sweet potato product of the complete health of 225g, 450g and 775g with water
Plant and be：Xu's potato 32) epidermis cleans up, and then naturally dry to epidermis is dried, then irradiates 3h under uviol lamp.
(2) from the clean transparent plastic box that height is 20cm, layer overlay thickness is 7cm vermiculite in plastic casing,
3 pieces of sweet potatoes of pretreatment in step (1) are placed on vermiculite, are then covered with the vermiculite that a layer thickness is 3cm again, it is exposed on vermiculite
The sweet potato in face is highly 3cm；Plastic casing is placed in insect cage (with embodiment 1)；The particle size of the vermiculite is 1~3mm；
The water content of the vermiculite is 70~80%.
(3) different Bradysia fungus gnat (Bradysia difformis) female adult pest 100 and male imago 100 are accessed in insect cage
Head, insect cage is placed in biochemical cultivation case, temperature be 24 DEG C, illumination condition 16L：8D, relative humidity are 70% condition
Lower raising 145 days (above-mentioned 3 pieces of sweet potatoes can breed 5 generations different Bradysia fungus gnat).
(4) treat that by the space-time of feeding one, foster worm soon is put into the clean container equipped with new sweet potato and vermiculite for sweet potato pulp
In cage, the sweet potato totally by feeding is replaced, continues to breed different Bradysia fungus gnat.
Observation and statistical method are the same as embodiment 1.
As a result different Bradysia fungus gnat is raised with sweet potato, its survival rate reaches 91.0%, and percentage of pupation reaches 92.6%, and eclosion rate reaches
To 97.8%, single female egg laying amount reaches 109.Illustrate that sweet potato disclosure satisfy that the nutrition of the different each growth phase of Bradysia fungus gnat needs
Ask, the whole history of life such as different Bradysia fungus gnat can complete spawning, larval growth well, pupate, sprout wings, the inventive method raising
The effect of different Bradysia fungus gnat is good；And the uniformity and stability of the different Bradysia fungus gnat provided are good；Extensive raising can be achieved.
In addition, raw material is easy to get, method is simple, and raw material and cost of labor are low.
Embodiment 3 raises mushroom fly experiment by feed of sweet potato
Carry out as follows：
(1) feed pre-processes：With water, by the complete disease-free sweet potato of health of 345g, 265g and 635g tri-, (sweet potato variety is
Ji potato 98) epidermis cleans up, and then naturally dry to epidermis is dried, then irradiates 4h under uviol lamp.
(2) from the clean normal transparent plastics basin that height is 20cm, layer overlay flood is all 7cm in plastics basin
Vermiculite, the 3 pieces of sweet potatoes pre-processed in step (1) are placed on vermiculite, are then covered with the leech that a layer thickness is 5cm again
Stone, the exposed sweet potato on vermiculite layers are highly 4cm；Plastic tub is placed in insect cage；The particle size of the vermiculite is 1
~3mm, the water content of vermiculite is 70~80%.
(3) mushroom fly (Bradysia minpleuroti) female adult pest 100 and male imago 100 are accessed in insect cage
Head, be placed in biochemical cultivation case, temperature be 26 DEG C, illumination condition 16L：8D, relative humidity raise 148 under the conditions of being 80%
My god (above-mentioned 3 pieces of sweet potatoes can breed 5 generation mushroom flies).
(4) sweet potato pulp is treated soon by the complete space-time of feeding one, with the transparent plastic basin equipped with fresh sweet potato and vermiculite
It is put into insect cage, replaces the sweet potato totally by feeding, continue to breed mushroom fly.
Observation and statistical method are the same as embodiment 1.
As a result mushroom fly is raised with sweet potato, its survival rate reaches 92.5%, and percentage of pupation reaches 94.3%, and eclosion rate reaches
93.4%, single female egg laying amount reaches 116.The above results illustrate that the inventive method raising mushroom fly effect is good；And gained
The uniformity and stability of larva are good, and it is with a high credibility to do biologic test with it, highly reliable；It it also allows to raise on a large scale
It is possibly realized.Meanwhile the inventive method raw material is easy to get, method is simple, raw material and cost of labor are low.
Embodiment 4 is raised the strict eye fungus gnat of flat mushroom as feed using sweet potato and tested
Carry out as follows：
(1) pre-process：By three weight it is disease-free sweet potato (the sweet potato product of the complete health of 450g, 665g and 795g with water
Plant and be：Xu's potato 32) epidermis cleans up, and then naturally dry to epidermis is dried, then irradiates 4h under uviol lamp.
(2) from the clean plastic casing that height is 20cm, layer overlay thickness is 7cm vermiculite in plastic casing, in leech
The 3 pieces of sweet potatoes pre-processed in step (1) are placed on stone, are then covered with the vermiculite that a layer thickness is 5cm again, it is exposed in leech
Sweet potato above stone is highly 4cm；Plastic casing is placed in insect cage (with embodiment 1)；The particle size of the vermiculite be 1~
3mm, the water content of vermiculite is 70~80%.
(3) the strict eye fungus gnat of flat mushroom (Lycoriella pleuroti) female adult pest 100 and male imago are accessed in insect cage
100, it is placed in biochemical cultivation case, is 26 DEG C in temperature, illumination condition 16L：8D, relative humidity are carried out under the conditions of being 80%
Raise 176 days (above-mentioned 3 pieces of sweet potatoes can breed the strict eye fungus gnat of 6 generation flat mushrooms).
(4) sweet potato pulp is treated soon by the complete space-time of feeding one, with the normal transparent plastics equipped with fresh sweet potato and vermiculite
Basin is put into insect cage, replaces the sweet potato totally by feeding, continues to breed the strict eye fungus gnat of flat mushroom.
Observation and statistical method are the same as embodiment 1.
As a result the strict eye fungus gnat of flat mushroom is raised with sweet potato, its survival rate is 93.5%, percentage of pupation 91.5%, and eclosion rate is
95.0%, single female egg laying amount is 113.It is good that the above results illustrate that the inventive method raising flat mushroom goes through a fungus gnat effect；And institute
Larva uniformity and stability it is good, it is with a high credibility to do biologic test with it, it is highly reliable；It it also allows to raise on a large scale
Support and be possibly realized.Meanwhile the inventive method raw material is easy to get, method is simple, raw material and cost of labor are low.
Claims (10)
1. application of the sweet potato as feed in fungus gnat artificial feeding.
2. application according to claim 1, it is characterised in that described fungus gnat refers to a Sciaridae
(Mycetophilidae) Bradysia Sciara (Bradysia) or strict eye Sciara (Lycoriella) insect.
3. application according to claim 2, it is characterised in that described Bradysia Sciara insect refers to Bradysia odoriphaga
(Bradysia odoriphaga), different Bradysia fungus gnat (Bradysia difformis), mushroom fly (Bradysia
Minpleuroti), Fujian mushroom Bradysia fungus gnat (Bradysia minpleuroti) or Lu Shi Bradysias fungus gnat (Bradysia luhi).
4. application according to claim 2, it is characterised in that described strict eye Sciara insect refers to the strict eye fungus gnat of flat mushroom
(Lycoriella pleuroti) or the strict eye fungus gnat of cloud mushroom (Lycoriella yunpleuroti).
A kind of 5. method that fungus gnat is raised using sweet potato, it is characterised in that comprise the following steps：
(1), pre-process：The sweet potato epidermis of fresh and healthy is cleaned up, then naturally dry to epidermis is dried, then in uviol lamp
2~4h of lower irradiation；
(2), from the clean container that height is 15~20cm, the vermiculite that thickness is 5~7cm is spread in container, then in leech
1~3 piece of the sweet potato of step (1) pretreatment is placed above in stone, then is covered with the vermiculite that a layer thickness is 3~5cm, exposed in vermiculite layers
Sweet potato above is highly 2~4cm；Container is placed in insect cage；
(3) the male and female adult population of same fungus gnat, is accessed in insect cage, then step is equipped with to the insect cage of fungus gnat adult
It is placed in again in biochemical cultivation case, is 24~26 DEG C in temperature, illumination 16L：Raised under the conditions of 8D；
(4), treat that sweet potato pulp will be by the complete space-time of feeding one, in Adult worms producting eggs peak period, by the appearance equipped with fresh sweet potato and vermiculite
Device is put into insect cage, replaces the potato wedge totally by feeding, is repeated above step and is continued to raise.
6. according to the method for claim 4, it is characterised in that the size of the sweet potato is 200~800g/.
7. according to the method for claim 4, it is characterised in that the container described in its step (2) is transparent plastic casing,
Or it is transparent plastics basin.
8. according to the method for claim 4, it is characterised in that the particle size of vermiculite described in its step (2) be 1~
3mm；The water content of described vermiculite is 70~80%.
9. according to the method for claim 4, it is characterised in that the ratio of the female male imago described in its step (3) be 1~
2：1.
10. according to the method for claim 4, it is characterised in that the fungus gnat described in its step (3) refers to a Sciaridae
(Mycetophilidae) insect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710998444.2A CN107736307B (en) | 2017-10-24 | 2017-10-24 | Method for feeding mushroom mosquitoes by using sweet potatoes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710998444.2A CN107736307B (en) | 2017-10-24 | 2017-10-24 | Method for feeding mushroom mosquitoes by using sweet potatoes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107736307A true CN107736307A (en) | 2018-02-27 |
| CN107736307B CN107736307B (en) | 2020-04-07 |
Family
ID=61236850
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710998444.2A Active CN107736307B (en) | 2017-10-24 | 2017-10-24 | Method for feeding mushroom mosquitoes by using sweet potatoes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107736307B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008031050A (en) * | 2006-07-26 | 2008-02-14 | Earth Chem Corp Ltd | Material for breeding insect |
| KR20130098765A (en) * | 2012-02-28 | 2013-09-05 | 주식회사 내츄로바이오텍 | Plant-derived insecticidal composition against mushroom fly containing plant-derived oil |
| CN104509494A (en) * | 2014-12-16 | 2015-04-15 | 山东省农业科学院植物保护研究所 | Artificial rearing method of tested Chinese chive maggot |
| CN104542498A (en) * | 2014-12-16 | 2015-04-29 | 山东省农业科学院植物保护研究所 | Artificial feeding method for tested bradysia odoriphaga |
| CN104798732A (en) * | 2015-05-11 | 2015-07-29 | 福建农林大学 | Method for large-batch artificial feeding of Macrocheles glaber |
| CN105145488A (en) * | 2015-07-08 | 2015-12-16 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring Chinese chive maggot larvae |
| CN205082493U (en) * | 2015-11-11 | 2016-03-16 | 长江大学 | Leek is breed bottle of eye fungus gnat late |
| CN105918256A (en) * | 2016-03-23 | 2016-09-07 | 中国农业科学院蔬菜花卉研究所 | Simple method for artificial feeding of chinese chive maggots |
| KR20170057905A (en) * | 2015-11-17 | 2017-05-26 | 농업회사법인주식회사디에이치씨 | Cultivation method of Sparassis crispa |
| CN107173336A (en) * | 2017-07-14 | 2017-09-19 | 山东省农业科学院植物保护研究所 | A kind of fragrant-flowered garlic maggot method for breeding |
-
2017
- 2017-10-24 CN CN201710998444.2A patent/CN107736307B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008031050A (en) * | 2006-07-26 | 2008-02-14 | Earth Chem Corp Ltd | Material for breeding insect |
| KR20130098765A (en) * | 2012-02-28 | 2013-09-05 | 주식회사 내츄로바이오텍 | Plant-derived insecticidal composition against mushroom fly containing plant-derived oil |
| CN104509494A (en) * | 2014-12-16 | 2015-04-15 | 山东省农业科学院植物保护研究所 | Artificial rearing method of tested Chinese chive maggot |
| CN104542498A (en) * | 2014-12-16 | 2015-04-29 | 山东省农业科学院植物保护研究所 | Artificial feeding method for tested bradysia odoriphaga |
| CN104798732A (en) * | 2015-05-11 | 2015-07-29 | 福建农林大学 | Method for large-batch artificial feeding of Macrocheles glaber |
| CN105145488A (en) * | 2015-07-08 | 2015-12-16 | 山东省农业科学院植物保护研究所 | Method for trapping and monitoring Chinese chive maggot larvae |
| CN205082493U (en) * | 2015-11-11 | 2016-03-16 | 长江大学 | Leek is breed bottle of eye fungus gnat late |
| KR20170057905A (en) * | 2015-11-17 | 2017-05-26 | 농업회사법인주식회사디에이치씨 | Cultivation method of Sparassis crispa |
| CN105918256A (en) * | 2016-03-23 | 2016-09-07 | 中国农业科学院蔬菜花卉研究所 | Simple method for artificial feeding of chinese chive maggots |
| CN107173336A (en) * | 2017-07-14 | 2017-09-19 | 山东省农业科学院植物保护研究所 | A kind of fragrant-flowered garlic maggot method for breeding |
Non-Patent Citations (4)
| Title |
|---|
| HEUNG YONG JEON 等: "Development of Simple Monitoring Techniques of Fungus Gnats, Bradysia agrestis (Diptera: Sciaridae) Larva and Adult in Sweet Pepper Greenhouse", 《KOREAN J. APPL. ENTOMOL.》 * |
| 杨峰山 等: "韭菜迟眼蕈蚊在不同寄主上产卵及对LED光趋性的研究", 《中国蔬菜》 * |
| 王梓清 等: "厉眼蕈蚊简易大量饲养方法", 《中国食用菌》 * |
| 陈浩 等: "韭菜迟眼蕈蚊规模化饲养技术", 《应用昆虫学报》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107736307B (en) | 2020-04-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103385220B (en) | Scale breeding method for encarsia formosa | |
| CN104642267B (en) | A kind of industrialization breeding method of aphidius gifuensis | |
| CN101569297B (en) | Breeding method of pyemotes zhonghuajia and application thereof in pest biological control | |
| CN101810257A (en) | Minitype space farm-food regeneration system capable of adopting insect to cycle all biological waste | |
| CA2899592C (en) | Mite rearing methods | |
| CN105746435B (en) | A kind of method using rice moth egg breeding chrysopa septempunctata larva | |
| CN106900656B (en) | A kind of cigarette fleahopper industrial production method | |
| CN109328819B (en) | Natural circulation full-ecological efficient farm and natural circulation utilization method of biological resources | |
| Babytskiy et al. | New findings of pest sciarid species (Diptera, Sciaridae) in Ukraine, with the first record of Bradysia difformis | |
| CN109769755A (en) | A kind of simple and fast propagation method of spotted wing drosophila pupa parasitic wasp hair angle clavicorn serphid | |
| CN104957116B (en) | A kind of method for trapping fragrant-flowered garlic maggot Adult worms producting eggs | |
| CN107173336A (en) | A kind of fragrant-flowered garlic maggot method for breeding | |
| Khan et al. | Pests of walnut | |
| CN104996723A (en) | Formula and preparation method of artificial feeding feed for Holotrichia parallela | |
| CN108064582B (en) | Facility for producing pollution-free agricultural products | |
| Burakowski | Laboratory methods for rearing soil beetles (Coleoptera) | |
| Kamalakannan et al. | Mushrooms–A hidden treasure | |
| CN107182943A (en) | A kind of method of indoor mass rearing Bradysia fungus gnat | |
| Akter et al. | Effect of different spawn seed on growth and varieties of the Oyster mushrooms (Pleurotus spp.) | |
| CN107736307A (en) | A kind of method that fungus gnat is raised using sweet potato | |
| CN105918256A (en) | Simple method for artificial feeding of chinese chive maggots | |
| CN111034684A (en) | Semitrella semipenguii seed conservation and rejuvenation breeding method | |
| Popenoe | Mushroom pests and how to control them | |
| Marri et al. | Growing Pleurotus ostreatus (Ex. Fr) Kummer Using Gamma Radiation In Southern Ghana And Its Associated Pests | |
| Laichattiwar et al. | Biology and morphometry of shoot and fruit borer, Leucinodes orbonalis (Guenee) on Brinjal, Solanum melongena (Mill.) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |