CN104509494B - Artificial rearing method of tested Chinese chive maggot - Google Patents
Artificial rearing method of tested Chinese chive maggot Download PDFInfo
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- CN104509494B CN104509494B CN201410783954.4A CN201410783954A CN104509494B CN 104509494 B CN104509494 B CN 104509494B CN 201410783954 A CN201410783954 A CN 201410783954A CN 104509494 B CN104509494 B CN 104509494B
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- 238000000034 method Methods 0.000 title claims abstract description 50
- 230000000384 rearing effect Effects 0.000 title claims abstract description 17
- 244000003377 Allium tuberosum Species 0.000 title abstract 6
- 235000005338 Allium tuberosum Nutrition 0.000 title abstract 6
- 239000004576 sand Substances 0.000 claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000009395 breeding Methods 0.000 claims description 23
- 230000001488 breeding effect Effects 0.000 claims description 21
- 241000607479 Yersinia pestis Species 0.000 claims description 15
- 230000009571 larval growth Effects 0.000 claims description 10
- 238000009835 boiling Methods 0.000 claims description 8
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- 235000013601 eggs Nutrition 0.000 claims description 8
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- 244000295724 Allium chinense Species 0.000 abstract 1
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- 235000011613 Pinus brutia Nutrition 0.000 description 1
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- RLBIQVVOMOPOHC-UHFFFAOYSA-N parathion-methyl Chemical group COP(=S)(OC)OC1=CC=C([N+]([O-])=O)C=C1 RLBIQVVOMOPOHC-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an artificial large-scale rearing method of tested insects, in particular to an artificial mass rearing method of tested Chinese chive maggot. Potatoes replace existing Chinese chive, green Chinese onion and artificial feed, and a fine sand moisturizing method replaces an agar moisturizing method and an artificial water adding moisturizing method. Compared with an existing tested Chinese chive maggot rearing method, the survival rate, the pupation rate, the emergence rate and the egg number per female of the artificial rearing method are equal to those of the existing tested Chinese chive maggot rearing method, but cost is saved, artificial operation steps are decreased, and favorable conditions are provided for normal supply of the tested Chinese chive maggot.
Description
Technical field
The present invention relates to a kind of rearing method for examination insecticide, particularly to a kind of artificial breeding side for examination fragrant-flowered garlic maggot
Method.
Background technology
Fragrant-flowered garlic maggot Bradysia odoriphaga, is the No.1 insect of Folium Allii tuberosi, directly threaten and govern Folium Allii tuberosi safety in production and
Sell.For a long time, vegetable grower relies primarily on high poison or hypertoxic insecticide, such as parathion-methyl, thimet (3911), Bayer 71628, first
Base different sulfur phosphorus, Furadan etc. prevents and treats this obstinate insect, or even arbitrarily increases dose, causes urban and rural residents' poisoning or death by accident
Often occur.In recent years in Dezhou, Boxing, the ground such as Ningjin repeatedly occurs diet to cause poisoning with Folium Allii tuberosi, wherein for Shandong Province
Most serious cause more than 70 people's acute poisonings, the residual hazard problem of Folium Allii tuberosi has caused worry and the concern of society, it has also become vegetable
Problem anxious to be resolved in dish.
With the raising to the concern of health level and to quality of life requirement for the people, pollution-free vegetable life in pollution-free food
Produce and the cry of agricultural sustainable development is more and more stronger.The basic conception that pollution-free vegetable produces and technical requirements are " for protecting
Demonstrate,prove certain yield and benefit, not to the not exceeded premise of toxic residua matter in production environment generation pollution and agricultural product body
Down it is allowed to limitation is using the chemical substance limiting." that is, pollution-free vegetable produces it is allowed to limitation uses some chemical fertilizer,
Low-toxin farm chemicals etc., but the toxic residua matter in vegetable not can exceed that state-set standard.Enter most, Beijing, the big city such as Shanghai,
Special requirement and regulation have all been done to Folium Allii tuberosi No-harmful apple orchard.
Large scale artificial breeding's technology for trying insecticide is one of important means of modern Pest control research.Fragrant-flowered garlic maggot is as grinding
Study carefully and controlling object, especially carry out Biological control and Drug resistance measures the research work such as steriliation by irradiation, study its synthetic
Feedstuff and mass rearing reproduction technique, solve test worm supply problem, are highly important to Folium Allii tuberosi No-harmful apple orchard, existing fragrant-flowered garlic
Maggot breeding technology is generally traditional fragrant-flowered garlic maggot raising and uses Folium Allii tuberosi section, i.e. Folium Allii tuberosi collection is returned, and goes the removal of impurity, peeling, Folium Allii tuberosi is ploughed and cuts
Become 4-6mm segment, eat for fragrant-flowered garlic maggot larva, all using natural Folium Allii tuberosi as feedstuff in whole feeding process, due to existing
All cannot thoroughly be removed with certain pesticide residues in Folium Allii tuberosi, the raising so allowing for fragrant-flowered garlic maggot cannot be protected safely
Card, the murder by poisoning for fragrant-flowered garlic maggot is larger, often causes the discontinuous of raising so that many generations of fragrant-flowered garlic maggot raise and cannot be carried out, so
The concordance for examination insecticide just cannot be ensured, reduce credibility and the representativeness of result of the test, and the fragrant-flowered garlic maggot sensitivity of gained
Difference, result of the test is inaccurate.
The method (zl200810238713.6) of fragrant-flowered garlic maggot raised by man-made feeds before our unit, it is possible to achieve interior is continuously
Disconnected, raising fragrant-flowered garlic maggot free from the influence of the external environment, the innoxious raising of fragrant-flowered garlic maggot can be realized by selecting raw material.But raise
Mainly reach the effect of moisturizing during supporting by filter paper, need to expend the moisturizing that manually carries out adding water in a large number, choose insect, choose
Ovum, wastes time and energy, and the agar adding in feedstuff, preservative etc. increased the cost of feedstuff.
Content of the invention
For the existing many deficiencies existing for examination fragrant-flowered garlic maggot method for breeding, the invention provides a kind of big rule for examination fragrant-flowered garlic maggot
Mould rearing method, instead of the existing man-made feeds using fresh leek, complicated as feedstuff with the Rhizoma Solani tuber osi being readily available
Method for breeding, after coordinating method for breeding of the present invention simultaneously, than existing for examination fragrant-flowered garlic its survival rate of maggot method for breeding,
Percentage of pupation, eclosion rate, single female egg laying amount is close, is that the regular supply for examination fragrant-flowered garlic maggot provides favourable conditions.
Rearing method for examination fragrant-flowered garlic maggot of the present invention, specifically includes following steps:
(1) feedstuff pretreatment: Rhizoma Solani tuber osi is peeled, cleaning, cut 1-2cm3Block, is scalded 10-15 second with boiling water；
(2) clean container for 10-25cm from height, in it, the fine sand of layer overlay 5-7cm, fine sand uniformly spreads
The feedstuff of one layer of pretreatment, then covers the thin fine sand of last layer 0.5-1cm again；
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, send out for Adult worms producting eggs and larval growth
Educate, until pupating, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container equipped with feedstuff and fine sand, repeat
Above step continues culture.
It is known that host's spectrum of fragrant-flowered garlic maggot is narrow, compare single, mainly the liliaceous plant of Herba Alii fistulosi Bulbus Allii class, fragrant-flowered garlic maggot is endangered
Evil Rhizoma Solani tuber osi has no report, the plant including Rhizoma Solani tuber osi and fruit, but inventor has been surprisingly found that under study for action, makees feedstuff using Rhizoma Solani tuber osi
Feed fragrant-flowered garlic maggot, good effect can be obtained, and Rhizoma Solani tuber osi be root crop it is easy to the four seasons preserve, price is low, pesticide residues
Low, the residual especially for the pesticide of conventional control fragrant-flowered garlic maggot is lower, and the murder by poisoning for fragrant-flowered garlic maggot is minimum it is ensured that raising is coherent
Property, and then ensure that the concordance for examination insecticide, enhance credibility and the representativeness of result of the test, result of the test is more accurate
Really.For the ease of fragrant-flowered garlic maggot, Rhizoma Solani tuber osi is taken food, need to carry out pretreatment to Rhizoma Solani tuber osi, will Rhizoma Solani tuber osi peel, cleaning, cut 1-2cm3
Block, is scalded 10-15 second with boiling water.Because this feedstuff is through pretreatment, further avoid the pesticide residues of conventional control fragrant-flowered garlic maggot
Problem, this ensures that there the safety in feeding process, ensure that breeding out each will not be due to pesticide etc. for fragrant-flowered garlic maggot
Stimulation component and develop immunity to drugs, improve fragrant-flowered garlic maggot for test sensitivity, thus improve biotic experiment accuracy rate and
Verity.Simultaneously because reduce relying on so that will not be due to the seasonality of Folium Allii tuberosi in feeding process for natural Folium Allii tuberosi
Stop production and cause the anxiety of feedstuff it is achieved that serialization is raised；Rhizoma Solani tuber osi in the method, the homogeneity of its quality and stability have
Greatly improve, this ensures that there the concordance of the fragrant-flowered garlic maggot quality raised out, fragrant-flowered garlic will not be caused due to the relation of feedstuff
The big rise and big fall of maggot quality, for biotic experiment, this concordance is of crucial importance.
Above-mentioned feeding process is 25-28 DEG C in temperature, and relative humidity is carried out, due to children in the box for feeding pests for 75-85%
During worm grows into adult, temperature and relative humidity have decisive role, in order to prevent fragrant-flowered garlic maggot retarded growth, will
Temperature and relative humidity control in above-mentioned scope.
The present invention has abandoned the method raised using means such as filter paper, agar, absorbent cottons of routine, and employs thin
Sand, fine sand not only can be developed for fragrant-flowered garlic maggot larval growth provides place, provides moist environment for larval growth, and moistening effect
Good.Fine sand provides place for Adult worms producting eggs simultaneously, it is to avoid the transfer of the ovum of conventional method for breeding and waste, improves breeding
Efficiency and quality；Fine sand cost is relatively low, and can recycle, and decreases the cost of raising.The fine sand that the present invention adopts contains
The water yield is 70%-80%, that is, hold agglomerating, but drips not water outlet and come, and handss pine group does not scatter entirely.Fine sand particle diameter of the present invention
For 0.25-0.35mm.
Rhizoma Solani tuber osi, this feedstuff rich in proteins and the various aminoacid of pretreatment are placed on fine sand, nutritious, can meet
Fragrant-flowered garlic maggot larval growth develop required it is ensured that during adult copulation nutrition sustainable supply, improve efficiency and the quality of breeding；With
When can recycle, decrease the cost of raising.
Clean container of the present invention is generally transparent plastic lunch box, and big plastics through sterilization after may also be employed
Cup, it is ensured that space needed for adult mate and oviposit, provides larger vivosphere to large quantities of larvas, and transparent plastics
Lunch box, observation that is easy to operate and facilitating experimenter.
Although existing method for breeding is resolutely different from method provided by the present invention, adopted in the past be all natural Folium Allii tuberosi,
Herba Alii fistulosi or synthetic feedstuff are higher to raise fragrant-flowered garlic maggot, the environmental condition requirement of raising, and because natural Folium Allii tuberosi itself is perishable
Rotten the shortcomings of, change feedstuff, keep humidity etc. to need to expend a large amount of manpowers.The fresh leek price in winter is higher, man-made feeds
Composition is various, virtually increased feeding cost.Above all these methods are all unfavorable for raising on a large scale at present, work as needs
When a large amount of test worm, it is difficult to supply.These make existing method for breeding there is larger defect；Disclosed raise with existing
Foster method is compared, and the method for the present invention formally there occurs radical change, employs modal Rhizoma Solani tuber osi and fine sand as former
Material raises fragrant-flowered garlic maggot so that having very big difference between method for breeding used by the present invention and existing method.Fine sand moisturizing is imitated
Really good, and appropriateness is more stable, decreases the number of times manually adding water, Rhizoma Solani tuber osi easily preserves, nutritious it is not necessary to increase extra
Composition, and almost there is no pesticide residues.Therefore this method have the advantages that low cost, simple to operate, save artificial, and
Bulky plastic lunch box provides, for the growth of fragrant-flowered garlic maggot, the space bred on a large scale, by feeding process in temperature can be just therefore
25-28 DEG C, relative humidity is carried out in the insectary for 75-85%, improves the growth rate of fragrant-flowered garlic maggot, permissible than prior art
Greater amount, turn out faster qualified health for examination fragrant-flowered garlic maggot.
In sum, after using above-mentioned method for breeding, than existing for examination fragrant-flowered garlic maggot method for breeding, gained fragrant-flowered garlic maggot quality
Homogeneity and stability are enhanced, and its survival rate, percentage of pupation, eclosion rate, single female egg laying amount and fresh leek, people
Work feedstuff quite, is that the regular supply for examination fragrant-flowered garlic maggot provides favourable conditions.Improve the sensitivity for test for the fragrant-flowered garlic maggot simultaneously,
Thus improve accuracy rate and the verity of biotic experiment, and this method for breeding, due to the Rhizoma Solani tuber osi that adopts, season is not limited
System, and do not affected by residual pesticide in Folium Allii tuberosi and quality, improve the continuity of raising.
Specific embodiment
Embodiment 1
A kind of rearing method for examination fragrant-flowered garlic maggot it is characterised in that: specifically include following steps:
(1) feedstuff pretreatment: Rhizoma Solani tuber osi is peeled, cleaning, cut 1-2cm3Block, is scalded 10 seconds with boiling water；
(2) clean container for 25cm, fine sand of layer overlay 5cm in it from height, fine sand uniformly spreads one layer
The feedstuff of pretreatment, then covers the thin fine sand of last layer 1cm again；
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, send out for Adult worms producting eggs and larval growth
Educate, until pupating, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container equipped with feedstuff and fine sand, repeat
Above step continues culture.
In described box for feeding pests, temperature is 25 DEG C, and relative humidity is 85%.
Embodiment 2
A kind of rearing method for examination fragrant-flowered garlic maggot it is characterised in that: specifically include following steps:
(1) feedstuff pretreatment: Rhizoma Solani tuber osi is peeled, cleaning, cut 1-2cm3Block, is scalded 15 seconds with boiling water；
(2) clean container for 10cm, fine sand of layer overlay 6cm in it from height, fine sand uniformly spreads one layer
The feedstuff of pretreatment, then covers the thin fine sand of last layer 0.5cm again；
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, send out for Adult worms producting eggs and larval growth
Educate, until pupating, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container equipped with feedstuff and fine sand, repeat
Above step continues culture.
In described box for feeding pests, temperature is 28 DEG C, and relative humidity is 75%.
Embodiment 3
A kind of rearing method for examination fragrant-flowered garlic maggot it is characterised in that: specifically include following steps:
(1) feedstuff pretreatment: Rhizoma Solani tuber osi is peeled, cleaning, cut 1-2cm3Block, is scalded 11 seconds with boiling water；
(2) clean container for 15cm, fine sand of layer overlay 7cm in it from height, fine sand uniformly spreads one layer
The feedstuff of pretreatment, then covers the thin fine sand of last layer 1cm again；
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, send out for Adult worms producting eggs and larval growth
Educate, until pupating, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container equipped with feedstuff and fine sand, repeat
Above step continues culture.
In described box for feeding pests, temperature is 26 DEG C, and relative humidity is 80%.
Embodiment 4
A kind of rearing method for examination fragrant-flowered garlic maggot it is characterised in that: specifically include following steps:
(1) feedstuff pretreatment: Rhizoma Solani tuber osi is peeled, cleaning, cut 1-2cm3Block, is scalded 12 seconds with boiling water；
(2) clean container for 20cm, fine sand of layer overlay 5cm in it from height, fine sand uniformly spreads one layer
The feedstuff of pretreatment, then covers the thin fine sand of last layer 1cm again；
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, send out for Adult worms producting eggs and larval growth
Educate, until pupating, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container equipped with feedstuff and fine sand, repeat
Above step continues culture.
In described box for feeding pests, temperature is 27 DEG C, and relative humidity is 75%.
Embodiment 5
A kind of rearing method for examination fragrant-flowered garlic maggot it is characterised in that: specifically include following steps:
(1) feedstuff pretreatment: Rhizoma Solani tuber osi is peeled, cleaning, cut 1-2cm3Block, is scalded 15 seconds with boiling water；
(2) clean container for 25cm, fine sand of layer overlay 7cm in it from height, fine sand uniformly spreads one layer
The feedstuff of pretreatment, then covers the thin fine sand of last layer 0.5cm again；
(3) female male imago is transferred in above-mentioned clean container, be placed in box for feeding pests, send out for Adult worms producting eggs and larval growth
Educate, until pupating, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container equipped with feedstuff and fine sand, repeat
Above step continues culture.
In described box for feeding pests, temperature is 25 DEG C, and relative humidity is 75%.
Comparative example 1
At identical conditions, using natural feed (Folium Allii tuberosi) and man-made feeds (raising described in zl200810238713.6
Material) feed fragrant-flowered garlic maggot, the survival rate of fragrant-flowered garlic maggot, percentage of pupation as the method described in the conventinal breeding method of feedstuff and embodiment 1, sprout wings
Rate and single female egg laying amount result are as follows:
By comparing above it can be seen that the fragrant-flowered garlic maggot of the method for breeding gained being adopted using embodiment 1, survival rate, pupate
Rate, eclosion rate is all raised beyond using natural feed (Folium Allii tuberosi) and man-made feeds (feedstuff described in zl200810238713.6) conduct
The fragrant-flowered garlic maggot that the conventinal breeding method of material is fed is although single female egg laying amount is slightly below existing method for breeding, but still can expire
The use further in sufficient later stage.And the present invention adopts Rhizoma Solani tuber osi as feedstuff, pesticide residues are few, safe and feeding cost is remote
Far below the method for breeding using man-made feeds (feedstuff described in zl200810238713.6).
Claims (2)
1. a kind of rearing method for examination fragrant-flowered garlic maggot it is characterised in that: specifically include following steps:
(1) feedstuff pretreatment: Rhizoma Solani tuber osi is peeled, cleaning, cut 1-2cm3Block, is scalded 10-15 second with boiling water；
(2) clean container for 10-25cm, fine sand of layer overlay 5-7cm in it from height, fine sand uniformly spreads one layer
The feedstuff of pretreatment, then covers the thin fine sand of last layer 0.5-1cm again；
(3) female male imago is transferred in above-mentioned clean container, is placed in box for feeding pests, develop for Adult worms producting eggs and larval growth,
Until pupating, treat that fragrant-flowered garlic maggot turns into adult, adult is transferred in the new clean container equipped with feedstuff and fine sand, more than repetition
Step continues culture；
Fine sand water content in described step (2) is 70%-80%.
2. method for breeding according to claim 1 it is characterised in that: in described box for feeding pests, temperature is 25-28 DEG C, relatively
Humidity is 75-85%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410783954.4A CN104509494B (en) | 2014-12-16 | 2014-12-16 | Artificial rearing method of tested Chinese chive maggot |
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| CN201410783954.4A CN104509494B (en) | 2014-12-16 | 2014-12-16 | Artificial rearing method of tested Chinese chive maggot |
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| CN104509494B true CN104509494B (en) | 2017-01-25 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104957116B (en) * | 2015-07-08 | 2018-06-15 | 山东省农业科学院植物保护研究所 | A kind of method for trapping fragrant-flowered garlic maggot Adult worms producting eggs |
| CN105145488B (en) * | 2015-07-08 | 2019-09-06 | 山东省农业科学院植物保护研究所 | A method of it traps and monitors bradysia odoriphaga larva |
| CN105918256B (en) * | 2016-03-23 | 2019-03-29 | 中国农业科学院蔬菜花卉研究所 | A kind of simple and easy method of artificial feeding bradysia odoriphaga |
| CN107736307B (en) * | 2017-10-24 | 2020-04-07 | 河北省农林科学院植物保护研究所 | Method for feeding mushroom mosquitoes by using sweet potatoes |
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