CN104824018A - Chinese chive maggot processing method - Google Patents
Chinese chive maggot processing method Download PDFInfo
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- CN104824018A CN104824018A CN201510267589.6A CN201510267589A CN104824018A CN 104824018 A CN104824018 A CN 104824018A CN 201510267589 A CN201510267589 A CN 201510267589A CN 104824018 A CN104824018 A CN 104824018A
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Abstract
The invention provides an insect prevention and treatment technology belonging to the field of plant protection and particularly relates to a Chinese chive maggot processing method. According to the method, a previous method which utilizes a lot of chemical pesticides to irrigate roots is changed, and a method for combining ozone water and the chemical pesticides is adopted; the disadvantages of pesticide residues, poor agricultural product safety, environment pollution and the like, caused by the fact that a lot of chemical pesticides are used, are avoided; and the strong oxidization reduction of the ozone water is utilized, and the Chinese chive maggot processing method has the characteristics of safety, no residues, no resistance and good insecticidal effect, and a novel prevention and treatment method is provided for preventing and treating underground insects, namely Chinese chive maggots, in the field.
Description
Technical field
The invention belongs to the insect control technology of plant protection art, relate to the processing method of a kind of fragrant-flowered garlic maggot.
Background technology
Fragrant-flowered garlic maggot---Bradysia odoriphaga Bradysia odoriphay is the No.1 insect of leek, directly threatens and govern safety in production and the sale of leek.For a long time, vegetable grower mainly relies on high poison or hypertoxic insecticide, as parathion-methyl, thimet (3911), acephatemet, the different sulphur phosphorus of methyl, this obstinate insect of the control such as Furadan, even arbitrarily strengthen dose, after causing urban and rural residents to eat leek, poisoning or death by accident often occurs.Shandong Province in recent years in Dezhou, repeatedly there is diet with leek and cause poisoning in Boxing, the ground such as Ningjin, wherein the most seriously cause more than 70 people's acute poisonings, the residual hazard problem of leek has caused worry and the concern of society, has become problem anxious to be resolved in vegetables.
Along with people are to the concern of the general level of the health and the raising to quality of life requirement, in pollution-free food, the cry of pollution-free vegetable production and agricultural sustainable development is more and more stronger.The basic conception that pollution-free vegetable produces and technical requirement are that " for ensureing certain output and benefit, under not producing to production environment the prerequisite that in pollution and agricultural product body, toxic residua matter does not exceed standard, Permissible limit uses the chemical substance limited." that is, pollution-free vegetable produces, and Permissible limit uses some chemical fertilizer, low-toxin farm chemicals etc., but the toxic residua matter in vegetables can not exceed state-set standard.Nearest Beijing, the big cities such as Shanghai, have all done special requirement and regulation to leek No-harmful apple orchard.
Fragrant-flowered garlic maggot body is little, and breeding rapidly, is lodged in soil, control difficulty.Current control fragrant-flowered garlic maggot, mainly based on chemical pesticide control, comprises and mixes soil and spread fertilizer over the fields, fill with the application methods such as root.Widely use the chemical agent such as phoxim, gamma cyhalothrin, some places even use 3911 contour poison forbidding medicaments.Use chemical agent and irrational insecticide-applying way to cause fragrant-flowered garlic maggot to reduce the susceptibility of medicament for many years continuously, pesticide resistance rises, and preventive effect reduces.Vegetable grower uses high poison, high residual medicament in a large number for preventing and treating fragrant-flowered garlic maggot for a long time, causes leek Pesticide Residues to exceed standard, and this is also the major issue that puzzlement leek produces.Along with food-safety problem is more and more subject to people's attention, need research and development badly and can reduce nontoxic, the free of contamination insecticide of the even alternative chemical pesticide of chemical pesticide consumption with Prevention technology fragrant-flowered garlic maggot.
Summary of the invention
Because of the series of problems that control fragrant-flowered garlic maggot uses chemical pesticide to cause in a large number in producing for existing leek, in order to the needs produced and live can be better met, develop the fragrant-flowered garlic maggot Prevention technology technology of new reduction Pesticide use amount, inventor is through long-term experiment, provide a kind of method adopting Ozone Water and chemical pesticide conbined usage, this method produces the upper prevention and controls adopting a large amount of chemical pesticide to fill with root before changing, and changes the drawback owing to using chemical pesticide to bring in a large number.Utilize Ozone Water and chemical pesticide conbined usage control fragrant-flowered garlic maggot, be conducive to reducing Pesticide use amount, reduce residue of pesticide, ensure crop production safety, and avoid fragrant-flowered garlic maggot and the series of problems such as to develop immunity to drugs.
Concrete technical scheme of the present invention is: adopt Ozone Water and chemical pesticide to process fragrant-flowered garlic maggot larva successively.Namely adopt low concentration ozone water pretreatment fragrant-flowered garlic maggot, wherein, adopt stomach toxicity co-toxicity method of tagging to carry out toxicity test to the examination worm hung after Ozone Water process more than 0.5-4 hour.
Described chemical pesticide is phoxim or gamma cyhalothrin.
Its concrete steps are:
(1) fragrant-flowered garlic maggot larva is immersed in Ozone Water, after 30min, it is taken out from Ozone Water, control solid carbon dioxide under room temperature and divide;
(2) former for chemical pesticide medicine is mixed with liquid; Clean filter paper to be laid in culture dish and medication liquid wetting; False for the leek soaked through liquid stem is sucked unnecessary liquid to be placed in culture dish; Then examination worm in the same size is provoked gently in culture dish with writing brush; Put into incubator to raise.
Wherein, the high-concentration ozone water preparing machine JOX-206 that Ozone Water uses Jinan Run Tu agricultural science and technology Co., Ltd to produce directly obtains.By adding the mode of ice cube or hot water, regulate the temperature of Ozone Water; Because the stability of Ozone Water is comparatively large by the impact of temperature, reduces the temperature of solution, can increase the stability of ozone, therefore Ozone Water is adjusted to lower temperature as far as possible, therefore the present invention adopts the mode putting into ice cube to reduce Ozone Water temperature.Fragrant-flowered garlic maggot winter and harm early spring the heaviest, the surface temperature of now facility leek cultivation at 10-20 DEG C, inventor try one's best control temperature when occurring with fragrant-flowered garlic maggot temperature consistent.
Owing to preventing and treating the application method of fragrant-flowered garlic maggot at present mainly to fill with root, therefore simulation this worm after Ozone Water is used may contact the mode of Ozone Water and design concrete method, and therefore the present invention adopts the method for ozone water immersion worm to carry out pretreatment to fragrant-flowered garlic maggot.
Because test finds that Ozone Water is about 20 DEG C time, in 20-30 minute, concentration is reduced to 0, therefore soaks the time controling of worm at 30 minutes.After leaching worm, taken out by insect from Ozone Water, control solid carbon dioxide divides, and does not drip.After control solid carbon dioxide, to feed foster insect according to normal temperature humidity and illumination.
Due to ozone glassware for drinking water strong oxidizing property, the effect of degrading pesticide can be played.So when conbined usage Ozone Water and chemical pesticide, consider both interval times, therefore the present invention's suggestion re-uses chemical pesticide after at least 0.5 hour, to ensure the action effect of chemical pesticide in use Ozone Water.
In the present invention, when carrying out pretreatment to fragrant-flowered garlic maggot larva, the usage amount of Ozone Water is 500mL, and ensure that consistency of ozone water is even, concentration reduces stable, if usage amount very little, although can not have fragrant-flowered garlic maggot larva, concentration is unstable, degrades faster.The clean filter paper 1-1.2mL liquid be laid in culture dish soaks, and both can provide sufficient liquid atmosphere for fragrant-flowered garlic maggot larval growth, and avoid again liquid to be too much drowned by fragrant-flowered garlic maggot larva; The false stem of leek is cut into about 1cm segment, takes out feed fragrant-flowered garlic maggot larva after soaking 30s in liquid.The culture dish diameter that the present invention adopts is 9cm, access examination worm 20 in each culture dish, described examination worm be 3 age fragrant-flowered garlic maggot larva.
After process terminates, condition of being put into by culture dish is temperature 25 ± 2 DEG C, the incubator of relative moisture 75 ± 5%, illumination 16L:8D is raised.
Adopt Ozone Water and chemical pesticide synergy to process fragrant-flowered garlic maggot, be reduce Pesticide use amount, reduce the effective way of residue of pesticide.The ozone glassware for drinking water strong oxidizing property that the present invention adopts, can destroy polypide body wall, make chemical agent better infiltrate polypide, and then better kill fragrant-flowered garlic maggot.And Ozone Water is unstable, reduction changes oxygen into very soon at normal temperatures, therefore does not pollute the environment with Ozone Water pretreatment fragrant-flowered garlic maggot, also can not produce any medicament residue in soil and crop.
Inventor finds that consistency of ozone water is too small, does not reach good fragmentation effect to fragrant-flowered garlic maggot; And the Ozone Water of high concentration not easily obtains, increase cost accounting, and high-concentration ozone water easily produces slight poisoning to it in the first vegetative period of leek, therefore consistency of ozone water of the present invention is 4-8ppm.
Although in actual production, have and be used alone Ozone Water to prevent and treat the method for fragrant-flowered garlic maggot, research finds that different vegetable is different to the endurance of ozone, excessive concentration is easily burnt vegetable leaf.And ozone concentration is too high can damage health.Research display, be chronically exposed in the air that ozone exceeds standard, ozone can damage lung's epidermal cell, and causes going down of lung functions.Therefore, be used alone Ozone Water to prevent and treat the method for fragrant-flowered garlic maggot, stable preventive effect may do not reached in the production of reality.Physical control and chemical control reasonable combination, will be an effective way of integrated control fragrant-flowered garlic maggot.
In the present invention, phoxim or the former medicinal acetone of gamma cyhalothrin are mixed with mother liquor by inventor, prepare the liquid of 5 concentration again according to the method being geometric ratio with 0.5 with the 0.1%Tween-80 aqueous solution, with reference to NY/T 1154.8-2007 process fragrant-flowered garlic maggot 3 instar larvae (Ozone Water of fragrant-flowered garlic maggot larva 4ppm carries out pretreatment), the reaction of being poisoned to death of examination worm is observed in 48h, calculate lethality, the results are shown in Table 1.
Table 1 chemical pesticide and Ozone Water conbined usage are to fragrant-flowered garlic maggot Contrast on effect
As can be seen from Table 1:
(1), when the concentration of described gamma cyhalothrin/phoxim liquid is 20mg/L, under ozone and chemical pesticide acting in conjunction, the lethality of fragrant-flowered garlic maggot just can reach more than 80%;
(2) when adopting Ozone Water and chemical pesticide synergy, reach close lethality, the usage amount of chemical pesticide obviously reduces than the usage amount of chemical pesticide when being used alone chemical pesticide.
In sum, the employing Ozone Water of the invention and drug combination are used for processing fragrant-flowered garlic maggot, processing cost can not only be reduced, slow down the drug-fast generation of fragrant-flowered garlic maggot, when its be applied to field fragrant-flowered garlic maggot is processed time, effectively can reduce the residue of pesticide in leek, improve people and eat the safety of leek, to the production promoting pollution-free food particularly green vegetables produce and have important practical significance.
Embodiment
Embodiment 1
A processing method for fragrant-flowered garlic maggot, its concrete steps are:
(1) preparation of Ozone Water: use the high-concentration ozone water preparing machine JOX-206 that Jinan Run Tu agricultural science and technology Co., Ltd produces, directly obtained Ozone Water.By adding the mode of ice cube, Ozone Water water temperature is regulated to be 15 ± 5 DEG C;
(2) the Ozone Water pretreatment of fragrant-flowered garlic maggot: fragrant-flowered garlic maggot 3 instar larvae is transferred in the self-control leaching worm net being covered with filter paper, measure 500mL concentration be 4ppm Ozone Water in beaker, the leaching worm net being placed with examination worm is immersed in beaker.After 30min, leaching worm net is taken out from beaker, under room temperature, hang 0.5h.
The Ozone Water of described Ozone Water to be the water temperature of preparation in step (1) the be 4ppm of 15 ± 5 DEG C.
(3) the chemical pesticide biologicall test of fragrant-flowered garlic maggot: 90% Phoxim original medicine acetone is mixed with mother liquor, prepare the liquid (1.25mg/L of 5 concentration again according to the method being geometric ratio with 0.5 with the 0.1%Tween-80 aqueous solution, 2.5mg/L, 5.0mg/L, 10.0mg/L, 20.0mg/L).Clean filter paper is laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False for leek stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid be placed on be covered with identical liquid process filter paper culture dish in, 2 sections, every ware.Then examination worm 20 in the same size is provoked gently in culture dish with writing brush.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L:D=16: 8.24h, 48h check result after medicine.
Embodiment 2
A processing method for fragrant-flowered garlic maggot, its concrete steps are:
(1) preparation of Ozone Water: use the high-concentration ozone water preparing machine JOX-206 that Jinan Run Tu agricultural science and technology Co., Ltd produces, directly obtained Ozone Water.By adding the mode of ice cube, regulate Ozone Water water temperature 15 ± 5 DEG C;
(2) the Ozone Water pretreatment of fragrant-flowered garlic maggot: fragrant-flowered garlic maggot 3 instar larvae is transferred in the self-control leaching worm net being covered with filter paper, measure 500mL concentration be 4ppm Ozone Water in beaker, the leaching worm net being placed with examination worm is immersed in beaker.After 30min, leaching worm net is taken out from beaker, under room temperature, hang 0.5h.
The Ozone Water of described Ozone Water to be the water temperature of preparation in step (1) the be 4ppm of 20 ± 5 DEG C.
(3) the chemical pesticide biologicall test of fragrant-flowered garlic maggot: the former medicinal acetone of 97% gamma cyhalothrin is mixed with mother liquor, prepare the liquid (1.25mg/L of 5 concentration again according to the method being geometric ratio with 0.5 with the 0.1%Tween-80 aqueous solution, 2.5mg/L, 5.0mg/L, 10.0mg/L, 20.0mg/L).Clean filter paper is laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False for leek stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid be placed on be covered with identical liquid process filter paper culture dish in, 2 sections, every ware.Then examination worm 20 in the same size is provoked gently in culture dish with writing brush.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L: D=16: 8.24h, 48h check result after medicine.
Comparative example 1
90% Phoxim original medicine is to the indoor virulence of fragrant-flowered garlic maggot 3 instar larvae
Former medicinal acetone is mixed with mother liquor, then prepares the liquid (1.25mg/L, 2.5mg/L, 5.0mg/L, 10.0mg/L, 20.0mg/L) of 5 concentration according to the method being geometric ratio with 0.5 with the 0.1%Tween-80 aqueous solution.Clean filter paper is laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False for leek stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid be placed on be covered with identical liquid process filter paper culture dish in, 2 sections, every ware.Then 3 instar larvaes in the same size are provoked gently in culture dish with writing brush.Every ware access examination worm 20, often processes repetition 3 times, establishes blank simultaneously.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L: D=16: 8.24h, 48h check result after medicine.
Comparative example 2
The former medicine of 97% gamma cyhalothrin is to the indoor virulence of fragrant-flowered garlic maggot 3 instar larvae
Former medicinal acetone is mixed with mother liquor, then prepares the liquid (1.25mg/L, 2.5mg/L, 5.0mg/L, 10.0mg/L, 20.0mg/L) of 5 concentration according to the method being geometric ratio with 0.5 with the 0.1%Tween-80 aqueous solution.Clean filter paper is laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False for leek stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid be placed on be covered with identical liquid process filter paper culture dish in, 2 sections, every ware.Then 3 instar larvaes in the same size are provoked gently in culture dish with writing brush.Every ware access examination worm 20, often processes repetition 3 times, establishes blank simultaneously.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L: D=16: 8.24h, 48h check result after medicine.
By comparative chemistry agricultural chemicals to the Toxicity Determination result of fragrant-flowered garlic maggot and Ozone Water and chemical pesticide to the synergy result of fragrant-flowered garlic maggot, find by concentration be 4ppm Ozone Water respectively with two kinds of chemical pesticide Combined Treatment fragrant-flowered garlic maggots, chemical pesticide used is to the LC of fragrant-flowered garlic maggot
50value is respectively lower than the LC of the alone process of chemical pesticide
50value.Result shows, Ozone Water is combined with above-mentioned chemical pesticide and prevented and treated fragrant-flowered garlic maggot and have better effect.The results are shown in Table 2.
The action effect of table 2 different agents
As seen from Table 1:
(1) in the present invention through Ozone Water pretreated fragrant-flowered garlic maggot 3 instar larvae to the LC of phoxim 24h, 48h
50value is respectively 6.516 and 3.520mg/L, respectively lower than the LC of alone phoxim 24h, 48h
50value 12.146 and 7.734mg/L.
(2) the present invention through Ozone Water pretreated fragrant-flowered garlic maggot 3 instar larvae to the LC of 24h, 48h of gamma cyhalothrin
50value is respectively 4.445 and 2.657mg/L, respectively lower than the LC of alone gamma cyhalothrin 24h, 48h
50value 6.474 and 4.194mg/L.
By contrasting discovery above, the present invention adopts Ozone Water and chemical pesticide to the synergy of fragrant-flowered garlic maggot compared with the method for the chemical pesticide control fragrant-flowered garlic maggot of routine, and Ozone Water is combined with chemical pesticide and prevented and treated fragrant-flowered garlic maggot and have better effect.This method is conducive to reducing Pesticide use amount, reduces residue of pesticide, ensures crop production safety, and avoids fragrant-flowered garlic maggot and the series of problems such as to develop immunity to drugs.
Embodiment 3
A processing method for fragrant-flowered garlic maggot, its concrete steps are:
(1) the high-concentration ozone water preparing machine JOX-206 using Jinan Run Tu agricultural science and technology Co., Ltd to produce, directly obtained Ozone Water.By adding the mode of ice cube, regulate Ozone Water water temperature 15 DEG C;
(2) fragrant-flowered garlic maggot 3 instar larvae is transferred in the self-control leaching worm net being covered with filter paper, measure 500mL concentration be 7ppm Ozone Water in beaker, the leaching worm net being placed with examination worm is immersed in beaker.After 30min, leaching worm net is taken out from beaker, under room temperature, hang 0.5h.
The Ozone Water of described Ozone Water to be the water temperature of preparation in step (1) the be 7ppm of 15 DEG C.
(3) be the liquid of 20mg/L by the former medicine compound concentration of 97% gamma cyhalothrin, clean filter paper be laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid and is placed in culture dish, 2 sections, every ware.Then examination worm 20 in the same size is provoked gently in culture dish with writing brush.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L: D=16: 8.48h check result after medicine, lethality is 89.31%.
Embodiment 4
A processing method for fragrant-flowered garlic maggot, its concrete steps are:
(1) the high-concentration ozone water preparing machine JOX-206 using Jinan Run Tu agricultural science and technology Co., Ltd to produce, directly obtained Ozone Water.By adding the mode of ice cube, regulate Ozone Water water temperature 20 DEG C;
(2) fragrant-flowered garlic maggot 3 instar larvae is transferred in the self-control leaching worm net being covered with filter paper, measure 500mL concentration be 8ppm Ozone Water in beaker, the leaching worm net being placed with examination worm is immersed in beaker.After 30min, leaching worm net is taken out from beaker, under room temperature, hang 0.5h.
The Ozone Water of described Ozone Water to be the water temperature of preparation in step (1) the be 8ppm of 20 DEG C.
(3) be the liquid of 20mg/L by the former medicine compound concentration of 97% gamma cyhalothrin, clean filter paper be laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False for leek stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid and is placed in culture dish, 2 sections, every ware.Then examination worm 20 in the same size is provoked gently in culture dish with writing brush.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L: D=16: 8.48h check result after medicine, lethality is 90.03%.
Embodiment 5
A processing method for fragrant-flowered garlic maggot, its concrete steps are:
(1) the high-concentration ozone water preparing machine JOX-206 using Jinan Run Tu agricultural science and technology Co., Ltd to produce, directly obtained Ozone Water.By adding the mode of ice cube, regulate Ozone Water water temperature 10 DEG C;
(2) fragrant-flowered garlic maggot 3 instar larvae is transferred in the self-control leaching worm net being covered with filter paper, measure 500mL concentration be 5ppm Ozone Water in beaker, the leaching worm net being placed with examination worm is immersed in beaker.After 30min, leaching worm net is taken out from beaker, under room temperature, hang 0.5h.
The Ozone Water of described Ozone Water to be the water temperature of preparation in step (1) the be 5ppm of 10 DEG C.
(3) be the liquid of 20mg/L by the former medicine compound concentration of 97% gamma cyhalothrin, clean filter paper be laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False for leek stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid and is placed in culture dish, 2 sections, every ware.Then examination worm 20 in the same size is provoked gently in culture dish with writing brush.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L: D=16: 8.48h check result after medicine, lethality is 85.23%.
Embodiment 6
A processing method for fragrant-flowered garlic maggot, its concrete steps are:
(1) the high-concentration ozone water preparing machine JOX-206 using Jinan Run Tu agricultural science and technology Co., Ltd to produce, directly obtained Ozone Water.By adding the mode of ice cube, regulate Ozone Water water temperature 10 DEG C;
(2) fragrant-flowered garlic maggot 3 instar larvae is transferred in the self-control leaching worm net being covered with filter paper, measure 500mL concentration be 6ppm Ozone Water in beaker, the leaching worm net being placed with examination worm is immersed in beaker.After 30min, leaching worm net is taken out from beaker, under room temperature, hang 0.5h.
The Ozone Water of described Ozone Water to be the water temperature of preparation in step (1) the be 6ppm of 10 DEG C.
(3) be the liquid of 20mg/L by 90% Phoxim original medicine compound concentration, clean filter paper be laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False for leek stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid and is placed in culture dish, 2 sections, every ware.Then examination worm 20 in the same size is provoked gently in culture dish with writing brush.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L: D=16: 8.48h check result after medicine, lethality is 85.72%.
Embodiment 7
A processing method for fragrant-flowered garlic maggot, its concrete steps are:
(1) the high-concentration ozone water preparing machine JOX-206 using Jinan Run Tu agricultural science and technology Co., Ltd to produce, directly obtained Ozone Water.By adding the mode of ice cube, regulate Ozone Water water temperature 15 DEG C;
(2) fragrant-flowered garlic maggot 3 instar larvae is transferred in the self-control leaching worm net being covered with filter paper, measure 500mL concentration be 7ppm Ozone Water in beaker, the leaching worm net being placed with examination worm is immersed in beaker.After 30min, leaching worm net is taken out from beaker, under room temperature, hang 0.5h.
The Ozone Water of described Ozone Water to be the water temperature of preparation in step (1) the be 7ppm of 15 DEG C.
(3) be the liquid of 20mg/L by 90% Phoxim original medicine compound concentration, clean filter paper be laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False for leek stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid and is placed in culture dish, 2 sections, every ware.Then examination worm 20 in the same size is provoked gently in culture dish with writing brush.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L: D=16: 8.48h check result after medicine, lethality is 88.52%.
Embodiment 8
A processing method for fragrant-flowered garlic maggot, its concrete steps are:
(1) the high-concentration ozone water preparing machine JOX-206 using Jinan Run Tu agricultural science and technology Co., Ltd to produce, directly obtained Ozone Water.By adding the mode of ice cube, regulate Ozone Water water temperature 20 DEG C;
(2) fragrant-flowered garlic maggot 3 instar larvae is transferred in the self-control leaching worm net being covered with filter paper, measure 500mL concentration be 8ppm Ozone Water in beaker, the leaching worm net being placed with examination worm is immersed in beaker.After 30min, leaching worm net is taken out from beaker, under room temperature, hang 0.5h.
The Ozone Water of described Ozone Water to be the water temperature of preparation in step (1) the be 8ppm of 20 DEG C.
(3) be the liquid of 20mg/L by 90% Phoxim original medicine compound concentration, clean filter paper be laid in diameter 9cm culture dish, filter paper quantitatively drips 1.2mL liquid.False for leek stem is cut into about 1cm segment, takes out soak 30s in variable concentrations liquid after, blotting paper sucks unnecessary liquid and is placed in culture dish, 2 sections, every ware.Then examination worm 20 in the same size is provoked gently in culture dish with writing brush.Put into T=25 ± 2 DEG C, RH=75 ± 5%, raise in the incubator of L: D=16: 8.48h check result after medicine, lethality is 90.96%.
Claims (10)
1. a processing method for fragrant-flowered garlic maggot, is characterized in that: adopt Ozone Water and chemical pesticide to process fragrant-flowered garlic maggot larva successively.
2. the processing method of a kind of fragrant-flowered garlic maggot according to claim 1, is characterized in that: the concentration of described ozone ozone in water is 4-8ppm.
3. the processing method of a kind of fragrant-flowered garlic maggot according to claim 1, is characterized in that: described chemical pesticide is phoxim or gamma cyhalothrin.
4. the processing method of a kind of fragrant-flowered garlic maggot according to claim 1, is characterized in that: its concrete steps are:
(1) fragrant-flowered garlic maggot larva is immersed in Ozone Water, after 30min, it is taken out from Ozone Water, control solid carbon dioxide under room temperature and divide;
(2) former for chemical pesticide medicine is mixed with liquid; Clean filter paper to be laid in culture dish and medication liquid wetting; False for the leek soaked through liquid stem is sucked unnecessary liquid to be placed in culture dish; Then examination worm in the same size is provoked gently in culture dish with writing brush; Put into incubator to raise.
5. the processing method of a kind of fragrant-flowered garlic maggot according to claim 1, is characterized in that: the water temperature of Ozone Water is 10-20 DEG C.
6. the processing method of a kind of fragrant-flowered garlic maggot according to claim 4, is characterized in that: the concentration of described gamma cyhalothrin liquid is 20mg/L.
7. the processing method of a kind of fragrant-flowered garlic maggot according to claim 4, is characterized in that: the concentration of described phoxim liquid is 20mg/L.
8. the processing method of a kind of fragrant-flowered garlic maggot according to claim 4, is characterized in that: each culture dish access examination worm 20 in step (2).
9. the processing method of a kind of fragrant-flowered garlic maggot according to claim 3, is characterized in that: the incubator condition in step (2) is temperature is 25 ± 2 DEG C, relative moisture is 75 ± 5%, illumination is 16L:8D.
10. the processing method of a kind of fragrant-flowered garlic maggot according to claim 4, is characterized in that: the time interval between step (1) and step (2) is at least 0.5h.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106665211A (en) * | 2016-07-31 | 2017-05-17 | 中国农业科学院蔬菜花卉研究所 | Method for preventing and controlling Chinese chive maggots by jointly using ozone water and plastic agricultural films |
| CN111596048A (en) * | 2020-04-07 | 2020-08-28 | 浙江省农业科学院 | Application method of two insecticides |
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| CN111596048A (en) * | 2020-04-07 | 2020-08-28 | 浙江省农业科学院 | Application method of two insecticides |
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