CN102599355A - Artificial feed for lygus lucorum - Google Patents
Artificial feed for lygus lucorum Download PDFInfo
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- CN102599355A CN102599355A CN201110427148XA CN201110427148A CN102599355A CN 102599355 A CN102599355 A CN 102599355A CN 201110427148X A CN201110427148X A CN 201110427148XA CN 201110427148 A CN201110427148 A CN 201110427148A CN 102599355 A CN102599355 A CN 102599355A
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Abstract
The invention relates to a feed for artificially breeding lygus lucorum. The feed comprises fresh egg, yolk powder, lecithin, sucrose, yeast powder, sorbic acid and the like which serve as main components. The artificial feed is suitable for artificially breeding a large quantity of adults and nymphs of the lygus lucorum, the bred lygus lucorum has high survival rate and quick growth and development, the average egg laying amount of single female lygus lucorum is high, the feed has long quality guarantee period, the frequency of changing the feed is greatly reduced, time and labor are saved, and the cost of the feed is reduced. The feed has the characteristics of simplicity and strong practicability, and is easy to operate.
Description
Technical field
The present invention relates to insect and raise the field, specifically, relate to a kind of man-made feeds of raising green plant bug, more particularly, relate to a kind of man-made feeds of raising green plant bug adult and nymph.
Background technology
Green plant bug is a kind of important pests of Cotton in China of causing harm, and in the Yangtze river basin and the Huanghe valley distribution is arranged all.Green plant bug is long to the harm time of cotton, lasts till the term of opening bolls from seedling always, and the phase of causing harm reached more than 4 months.Green plant bug lunges the tender head of cotton, growing point, young flower bud, young bell with sucking mouth parts, draws juice.Cotyledon period growth bud point is killed, blackout, withered, not regrowth; After true leaf occurred, terminal bud was injured withered, and twig grows thickly, and became " bull is cotton "; Be the pore shape when tender leaf is killed, large tracts of land is broken behind the mounted blade, is commonly called as " broken leaf is crazy "; The main heart and the limit heart are killed, form " broom seedling " that branches and leaves are grown thickly; Bract was opened after bud was injured, and presented yellowish-brown earlier, withered then coming off; After cotton buds and bolls were killed, water stain spot appearred in light wound, and heavy cotton boll ossifys and comes off.
Soil of transgenic Bt cotton is behind China's establishing in large scale, and lepidoptera pests such as bollworm are controlled effectively, but because the minimizing significantly of cotton field chemical insecticide consumption and frequency of usage; Sucking pest population quantity such as green plant bug increase; Take place to endanger to be to increase the weight of trend year by year, the killed strain rate in general cotton field reaches 30%～40%, and serious reaches 60%～90%; The general underproduction 10%～20% in cotton field of being injured is up to more than 60%.Green plant bug has become the great insect of Cotton in China, Cotton in China is produced threaten greatly.Except that the cotton of causing harm, the green plant bug crops such as jujube, peach, apple, cherry, grape, tea tree of also causing harm have become the significant problem that influences China's various crop production safety.
The control of green plant bug at present mainly relies on chemical pesticide, and the extensive application of pesticide must cause the pollution of drug-fast rising of green plant bug and environment, and effective controlling way of therefore seeking outside the chemical prevention is the task of top priority.Need study many-sides such as the biology of green plant bug, ecology, physiology, toxicology, but these researchs all need a large amount of nutrition conditions and the test of worm neat and consistent in age to use worm.Therefore, must develop green plant bug feed formula efficiently, green plant bug is carried out artificial feeding, ensure for the research of green plant bug provides the examination worm.
The artificial feeding of external fleahopper class pest starts from the sixties in 20th century, raise the America tarnished plant bug with cowpea, cotton, clover at first, but effect is undesirable; Using kidney bean, potato bud and cauliflower afterwards instead raises; Effect is better, and survival rate reaches more than 80%, but because the freshness date of natural feed is too short; Time-consuming, researcher's man-made feeds that begin one's study.Originally liquid man-made feeds poor effect had been developed a kind of semi-solid man-made feeds afterwards, had reached comparatively desirable effect.Nymph survival rate with this prescription is raised China green plant bug has only 30%, is not suitable for growing of green plant bug.
China is relatively lagging behind aspect the development of fleahopper silk worm man-made feeds, once with host plant such as cotton, potato green plant bug, black striped plant bug, adelphocoris taeniophorus, alfalfa plant bug is raised, and uses for reference external way subsequently; Also attempted utilizing kidney bean (and as oviposition substrate) raise green plant bug with in black alfalfa plant bug, obtained effect preferably, can raise in indoor continuous multi-generation; But because the kidney bean fresh keeping time is short, residues of pesticides are high, and winter, the supply of kidney bean also was difficult to guarantee; Bring difficulty for the artificial feeding of green plant bug; The frequent more new feedstuff of kidney bean raising need simultaneously, time-consuming, cost is higher.On the basis that Song Guojing etc. (with the preferred green plant bug nymph of orthogonal experiment man-made feeds prescription, plant protection, 2010 the 6th phases) fill a prescription abroad; Developed green plant bug nymph man-made feeds prescription; Compare with kidney bean, go through phase prolongation 1 day with the green plant bug nymphal development that this prescription is raised, survival rate does not have tangible raising; And nearly 10 kinds of the important food ingredients that should fill a prescription, cost is higher; The low age nymph in 1,2 ages of green plant bug dislikes getting food, only to 3,4 advanced age in age nymph certain effect is arranged, also be inappropriate for the raising of green plant bug adult.
Therefore, development is suitable for the man-made feeds prescription of green plant bug nymph and adult, carry out a large amount of artificial feedings in the laboratory, and guarantee test is consistent with nutrition condition age with the worm of worm, becomes pressing for of current green plant bug research.
Summary of the invention
Main purpose of the present invention is to provide a kind of green plant bug man-made feeds.
Second purpose of the present invention is to provide a kind of method of preparing the green plant bug man-made feeds.
The 3rd purpose of the present invention is to provide a kind of method of using the green plant bug man-made feeds to raise green plant bug.
On the one hand; The present invention provides a kind of green plant bug man-made feeds; It comprises the composition of following proportioning: in every 200ml sterilized water, and fresh hen egg 20.0g-25.0g, powdery yolk 25.0g-30.0g, lecithin 1.0g-1.8g, sucrose 1.5g-2.0g, dusty yeast 0.2g-0.5g, sorbic acid 0.2g-0.3g, bright kidney bean filtrating 30ml-50ml.In preferred embodiments; The present invention provides a kind of green plant bug man-made feeds; It comprises the composition of following proportioning: in every 200ml sterilized water, and fresh hen egg 22.0g, powdery yolk 30.0g, lecithin 1.5g, sucrose 2.0g, dusty yeast 0.3g, sorbic acid 0.3g, bright kidney bean filtrating 40ml.
On the other hand; The present invention provides a kind of method of preparing above-mentioned green plant bug man-made feeds; Said method comprising the steps of: (1) takes by weighing powdery yolk, lecithin, sucrose, dusty yeast, the sorbic acid of said amount respectively; And each solid constituent that will take by weighing is dissolved in the beaker that the 200ml sterilized water is housed, and mixes; (2) fresh hen egg is shelled, mix, the fresh hen egg that takes by weighing said amount then places the beaker of step (1); (3) squeeze fresh kidney bean liquid, after the filtration, the bright kidney bean filtrating of measuring said volume adds the mixed liquor that step (2) makes, and stirs, and promptly obtains said feed.
On the other hand, the present invention provides a kind of method of using above-mentioned green plant bug man-made feeds to raise green plant bug, said method comprising the steps of: nymph or the adult of green plant bug inserted in (1) at the vessel of putting into above-mentioned green plant bug man-made feeds; The vessel that (2) will insert nymph or adult cover with poromeric material, place illumination box then, and temperature is 27 ℃ ± 1 ℃, and relative humidity is 60%-70%, and the photoperiod is L: D=14: 10; (3) every at a distance from feed of replacing in 3-5 days.In preferred embodiments, said green plant bug man-made feeds are with sealing the film parcel.In a more preferred embodiment, the said film that seals is that Parafilm seals film.
Behind the transgenic cotton against pests establishing in large scale, the status of cotton pest develops, and insects such as green plant bug rise to primary pest, cause harm to increase the weight of year by year.A large amount of standard examination worms are badly in need of in the research of green plant bug integrated control technique; And green plant bug man-made feeds formulation cost is high at present; The nymph survival rate is low, development duration prolongs, and feeding effect is undesirable, to the problems referred to above; Inventor of the present invention has developed above-mentioned novel green plant bug man-made feeds prescription through a large amount of experiment screenings.The active ingredient of this man-made feeds prescription has only 7 kinds; Lack than the desired active ingredient of existing prescription, feed cost reduces about 30%, not only can be used for a large amount of artificial feedings of green plant bug nymph; Also can be used for a large amount of artificial feedings of green plant bug adult; And survival rate improves about 8.89%, and the total development duration of nymph reduces 0.6 day, and the single female average egg laying amount of adult increases about 7.71%.This feed formula has stronger practicality and operability.
The specific embodiment
Those skilled in the art will make further detailed description to the present invention with the mode of embodiment below, so that can put into practice the present invention.Should be appreciated that and to adopt other embodiments, and can make suitable change and do not depart from the spirit or scope of the present invention.For fear of for making those skilled in the art can put into practice unnecessary details the present invention, specification possibly omit some known to those skilled in the art information.Therefore, below describe in detail and should not understand, and scope of the present invention is only defined by accompanying claims with restrictive meaning.
Employed each raw material among the following embodiment, except that particularly pointing out, all can be by commercially available acquisition.
Embodiment one:
1, feed preparation
By formulated green plant bug man-made feeds of the present invention, concrete preparation steps is following:
(1) takes by weighing powdery yolk 30g, lecithin 1.5g, sucrose 2.0g, dusty yeast 0.3g, sorbic acid 0.3g respectively with electronic balance, the solid constituent that takes by weighing is dissolved in the beaker that the 200ml sterilized water is housed, mix;
(2) fresh hen egg is shelled, mix, take by weighing the beaker that the 22g fresh hen egg places step (1);
(3) (for example with compact juicer; Nine positive soy bean milk making machine) directly squeeze fresh kidney bean liquid, after 2 layers of filtered through gauze, the bright kidney bean filtrating of measuring 40ml adds in the mixed liquor that above-mentioned steps (2) makes; Mixing and stirring promptly gets green plant bug man-made feeds of the present invention.
It will be appreciated that each step of said compound method is not limited to above order, carry out each step in proper order with other and also can realize the present invention.
Embodiment two:
2. test method
Seal the feed parcel that film (all there is sale in biological chemical reagent company) will prepare in right amount with Parafilm when feeding green plant bug, and with the fine rule wrapping, the assurance feed does not spill and gets final product.During raising, green plant bug can puncture seals film to obtain feed.
The existing formulated green plant bug man-made feeds of pressing Song Guojing etc. (with the preferred green plant bug nymph of orthogonal experiment man-made feeds prescription, plant protection, 2010 the 6th phases) simultaneously are as control treatment.The concrete composition of existing prescription is: sucrose 2.8g, beer yeast powder 0.25g, 50% hydromel 2.5g, egg 22.5g, wheat 10g, lima bean flour 30g, soy meal 2.5g, yolk 30g, soybean lecithin 1.5g, B B-complex 1.2g, water 164g.
2.1 nymph survival rate and nymph are always gone through the phase:
In plastics crisper (long 20cm, wide 15cm, high 8cm); Put into respectively with Parafilm and seal good an amount of the foregoing description one prepared prescription feed of the present invention of film parcel and existing prescription feed; Every box inserts adopt green plant bug that kidney bean raises first incubates 100 of nymphs; Every kind of feed connects 3 boxes, promptly carries out three times and repeats.The plastics crisper that inserts nymph is covered with four layers of hospital gauze, and restraint and go up rubber band, prevent the nymph escape, then the plastics crisper is placed illumination box, temperature is 27 ℃ ± 1 ℃, and relative humidity is 60%-70%, and the photoperiod is L: D=14: 10.Every day observed and recorded green plant bug nymph survival and development progress.Changed a feed in per 3 days.
2.2 the single female egg laying amount of adult
Raise first each the some head of nymph of incubating with above two kinds of feeds in above-mentioned same condition, until sprout wings.The adult that has just sprouted wings is inserted the insect box of placing fresh four seasons fresh kidney beans (oviposition substrate), and the male and female ratio is 1: 1, and every box inserts 50 pairs, changes fresh kidney bean every day.The beanpod that changes is checked top ovum amount under stereomicroscope (Japan, Olympus, model V123340-1), and writes down the egg laying amount of every box, and is all dead until all female worms.
Result of the test:
Can be known that by data in the table 1 compare with existing prescription, the green plant bug nymph survival rate of raising with the green plant bug man-made feeds of formulated of the present invention improves 8.89%, the total development duration of nymph has shortened 0.6 day, and single female egg laying amount increases by 7.71%.
The table 1 the present invention influence of feed of filling a prescription to green plant bug survival rate, development duration and egg laying amount
The result shows that green plant bug is on the feed of man-made feeds formulated of the present invention, and survival rate is high, grows fast, and egg laying amount increases, and explains that green plant bug man-made feeds of the present invention are suitable for the mass rearing of green plant bug nymph and adult.Green plant bug man-made feeds of the present invention have excellent economy and practicality.
In sum; More than being merely preferred embodiment of the present invention, is not to be used to limit protection scope of the present invention, therefore; All any modifications of within spirit of the present invention and principle, being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. green plant bug man-made feeds; It comprises the composition of following proportioning: in every 200ml sterilized water, and fresh hen egg 20.0g-25.0g, powdery yolk 25.0g-30.0g, lecithin 1.0g-1.8g, sucrose 1.5g-2.0g, dusty yeast 0.2g-0.5g, sorbic acid 0.2g-0.3g, bright kidney bean filtrating 30ml-50ml.
2. green plant bug man-made feeds as claimed in claim 1; Said each composition proportion is following: in every 200ml sterilized water, and fresh hen egg 22.0g, powdery yolk 30.0g, lecithin 1.5g, sucrose 2.0g, dusty yeast 0.3g, sorbic acid 0.3g, bright kidney bean filtrating 40ml.
3. prepare the method for the described green plant bug man-made feeds of claim 1, said method comprising the steps of:
(1) take by weighing powdery yolk, lecithin, sucrose, dusty yeast, the sorbic acid of said amount respectively, and each solid constituent that will take by weighing is dissolved in the beaker that the 200ml sterilized water is housed, mixes;
(2) fresh hen egg is shelled, mix, the fresh hen egg that takes by weighing said amount then places the beaker of step (1);
(3) squeeze fresh kidney bean liquid, after the filtration, the bright kidney bean filtrating of measuring said volume adds the mixed liquor that step (2) makes, and stirs, and promptly obtains said feed.
4. the method for using the described green plant bug man-made feeds of claim 1 to raise green plant bug said method comprising the steps of:
(1) inserts nymph or the adult of green plant bug at the vessel of putting into the described green plant bug man-made feeds of claim 1;
The vessel that (2) will insert nymph or adult cover with poromeric material, place illumination box then, and temperature is 27 ℃ ± 1 ℃, and relative humidity is 60%-70%, and the photoperiod is L: D=14: 10;
(3) every at a distance from feed of replacing in 3-5 days.
5. the method for raising green plant bug as claimed in claim 4, wherein said green plant bug man-made feeds are with sealing the film parcel.
6. the method for raising green plant bug as claimed in claim 5, the wherein said film that seals is that Parafilm seals film.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110427148 CN102599355B (en) | 2011-12-19 | 2011-12-19 | Artificial feed for lygus lucorum |
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| CN 201110427148 CN102599355B (en) | 2011-12-19 | 2011-12-19 | Artificial feed for lygus lucorum |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102771670A (en) * | 2012-08-03 | 2012-11-14 | 中国农业科学院植物保护研究所 | Artificial diet for predative natural enemy insect-true bugs |
| CN103250894A (en) * | 2013-02-04 | 2013-08-21 | 吉林省林业科学研究院 | Predatory nature enemy arma chinensis artificial feed preparation method |
| CN103271247A (en) * | 2013-06-03 | 2013-09-04 | 浙江省农业科学院 | Artificial feed of cyrtorhinus lividipennis |
| CN103609529A (en) * | 2013-09-18 | 2014-03-05 | 中国农业科学院植物保护研究所 | Method for evaluating influences of biological protein on apolygus lucorum |
| CN104351503A (en) * | 2014-10-16 | 2015-02-18 | 中国农业大学 | Artificial feeds for heteroptera:Miridae |
| CN104823924A (en) * | 2015-05-08 | 2015-08-12 | 河南省农业科学院植物保护研究所 | Method for determining toxicity of systemic insecticide to green plant bugs |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102048057A (en) * | 2010-11-24 | 2011-05-11 | 河南省农业科学院 | Artificial feed for Lygus lucorum and feeding method thereof |
-
2011
- 2011-12-19 CN CN 201110427148 patent/CN102599355B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102048057A (en) * | 2010-11-24 | 2011-05-11 | 河南省农业科学院 | Artificial feed for Lygus lucorum and feeding method thereof |
Non-Patent Citations (4)
| Title |
|---|
| WHITBEY, RM: "Green bean extract-induced oviposition site preference in laboratory reared western tarnished plant bug (Heteroptera：Miridae)", 《ENVIRONMENTAL ENTOMOLOGY》 * |
| 宋国晶等: "用正交试验法优选绿盲蝽若虫人工饲料配方", 《植物保护》 * |
| 陆宴辉等: "利用四季豆饲养盲蝽的方法", 《植物保护学报》 * |
| 魏书艳等: "光周期对绿盲蝽生长发育和繁殖的影响", 《江苏农业学报》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102771670A (en) * | 2012-08-03 | 2012-11-14 | 中国农业科学院植物保护研究所 | Artificial diet for predative natural enemy insect-true bugs |
| CN102771670B (en) * | 2012-08-03 | 2014-03-26 | 中国农业科学院植物保护研究所 | Artificial diet for predative natural enemy insect-true bugs |
| CN103250894A (en) * | 2013-02-04 | 2013-08-21 | 吉林省林业科学研究院 | Predatory nature enemy arma chinensis artificial feed preparation method |
| CN103271247A (en) * | 2013-06-03 | 2013-09-04 | 浙江省农业科学院 | Artificial feed of cyrtorhinus lividipennis |
| CN103271247B (en) * | 2013-06-03 | 2014-08-20 | 浙江省农业科学院 | Artificial feed of cyrtorhinus lividipennis |
| CN103609529A (en) * | 2013-09-18 | 2014-03-05 | 中国农业科学院植物保护研究所 | Method for evaluating influences of biological protein on apolygus lucorum |
| CN104351503A (en) * | 2014-10-16 | 2015-02-18 | 中国农业大学 | Artificial feeds for heteroptera:Miridae |
| CN104351503B (en) * | 2014-10-16 | 2019-01-29 | 中国农业大学 | A kind of green plant bug man-made feeds |
| CN104823924A (en) * | 2015-05-08 | 2015-08-12 | 河南省农业科学院植物保护研究所 | Method for determining toxicity of systemic insecticide to green plant bugs |
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