CN107927356A - A kind of gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation - Google Patents
A kind of gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation Download PDFInfo
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- CN107927356A CN107927356A CN201711272615.XA CN201711272615A CN107927356A CN 107927356 A CN107927356 A CN 107927356A CN 201711272615 A CN201711272615 A CN 201711272615A CN 107927356 A CN107927356 A CN 107927356A
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- 241001455175 Neoseiulus cucumeris Species 0.000 title claims abstract description 60
- 239000012530 fluid Substances 0.000 claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 15
- 210000002969 egg yolk Anatomy 0.000 claims abstract description 12
- 235000012907 honey Nutrition 0.000 claims abstract description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 10
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 239000012137 tryptone Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000011159 matrix material Substances 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 7
- 239000008272 agar Substances 0.000 claims description 7
- 241000382353 Pupa Species 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 210000000936 intestine Anatomy 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 241000406588 Amblyseius Species 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 210000000496 pancreas Anatomy 0.000 claims description 2
- 239000007858 starting material Substances 0.000 claims description 2
- 244000241257 Cucumis melo Species 0.000 claims 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims 1
- 241000238876 Acari Species 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 5
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 238000011161 development Methods 0.000 description 16
- 230000018109 developmental process Effects 0.000 description 16
- 102000002322 Egg Proteins Human genes 0.000 description 8
- 108010000912 Egg Proteins Proteins 0.000 description 8
- 239000006185 dispersion Substances 0.000 description 8
- 210000004681 ovum Anatomy 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 235000013305 food Nutrition 0.000 description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
- 240000001548 Camellia japonica Species 0.000 description 3
- 240000008067 Cucumis sativus Species 0.000 description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 235000018597 common camellia Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 241000927584 Frankliniella occidentalis Species 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 241000339374 Thrips tabaci Species 0.000 description 2
- 235000019728 animal nutrition Nutrition 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 230000008144 egg development Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000034004 oogenesis Effects 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- 241000611866 Tyrophagus putrescentiae Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
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- 238000010998 test method Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- -1 wherein Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to plant protection art, and in particular to a kind of gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation.The artificial modified feed includes tussah chrysalis tissue fluid, yeast extraction powder, tryptone, glucose, honey, fresh yolk and water.Provided by the present invention for the gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation, low in raw material price, can be effectively performed amblyseius cucumeris oudeman population indoor propagation, have positive facilitation to later Amblysiuss class Predatory Mites large-scale production.
Description
Technical field
The present invention relates to plant protection art, and in particular to a kind of amblyseius cucumeris oudeman population indoor propagation is gluey manually to be changed
Good feed.
Background technology
Amblyseius cucumeris oudeman foraging territories are wide, can prey on a variety of small insects such as thrips, tetranychid, aleyrodid, deficient in prey resource
Reactive volt-ampere hour, can also feeding pollen, honeydew and microorganism maintain population quantity, in addition, plant pollen is favourable while predation prey
In amblyseius cucumeris oudeman growth and development.
Different according to feeds utilized source, amblyseius cucumeris oudeman feed required when breeding can be divided into phytophagous feed (such as flower
Powder), carnivorous feed (such as flour mite) and man-made feeds.At present, amblyseius cucumeris oudeman large-scale breeding is mainly with tyrophagus putrescentiae and ellipse
Circle baking soda mite etc. carries out extensive artificial feeding for predation prey.The man-made feeds of existing amblyseius cucumeris oudeman mainly with pollen,
Inanimacy nutrient fodder based on yeast extraction powder, peptone etc., and be liquid-state feed, bred for amblyseius cucumeris oudeman
When, it is easy to cause mite and is drowned, be unfavorable for amblyseius cucumeris oudeman population indoor propagation, how provides in a kind of suitable amblyseius cucumeris oudeman room
Man-made feeds of Population breeding and preparation method thereof become those skilled in the art's technical problem to be solved.
The content of the invention
The defects of the purpose of the present invention is overcoming the prior art, there is provided a kind of suitable for amblyseius cucumeris oudeman population indoor propagation
Gluey artificial modified feed, it saves raw material, reduces production cost while reduce because being drowned caused by Predatory Mites death,
Improve reproductive efficiency.
The present invention provides the gluey artificial modified feed suitable for amblyseius cucumeris oudeman population indoor propagation, the improvement feed
Including matrix and agar powder, wherein the matrix includes：Tussah chrysalis tissue fluid, yeast extraction powder, tryptone, glucose, bee
Honey and fresh yolk, the weight of each base starting material are：10-15 parts of tussah chrysalis tissue fluid, 5-10 parts of powder of yeast extraction, pancreas egg
White 30-65 parts of 5-10 parts of peptone, 5-10 parts of glucose, 5-10 parts of honey, 10-15 parts of fresh yolk and distilled water.
The gluey artificial modified feed of the amblyseius cucumeris oudeman population indoor propagation of embodiment according to the present invention, its
In, the matrix includes：10 parts of tussah chrysalis tissue fluid, yeast extraction 5 parts of powder, 5 parts of tryptone, 5 parts of glucose, 5 parts of honey,
60 parts of 10 parts of fresh yolk and water.
The gluey artificial modified feed of the amblyseius cucumeris oudeman population indoor propagation of embodiment according to the present invention, its
In, the artificial modified feed includes the agar of gross weight 2%.
The gluey artificial modified feed of the amblyseius cucumeris oudeman population indoor propagation of embodiment according to the present invention, its
In, the tussah chrysalis tissue fluid is prepared by method comprising the following steps：
(1) after removing tussah chrysalis body wall and middle intestines, tussah chrysalis is ground at low temperature；
(2) and then by ground pupa tissue fluid centrifuge, Aspirate supernatant obtains tussah chrysalis tissue fluid.
The gluey artificial modified feed of the amblyseius cucumeris oudeman population indoor propagation of embodiment according to the present invention, its
In, in step (2), ground pupa tissue fluid is centrifuged into 1min under 6000rmp.
Present invention also offers the method for artificial propagation amblyseius cucumeris oudeman, and the described method includes use above-mentioned amblyseius cucumeris oudeman
The step of population indoor propagation is with gluey artificial modified feed feeding amblyseius cucumeris oudeman.
It is suitable for the gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation according to the present invention, it includes certain ratio
The tussah chrysalis tissue fluid of example, so as to improve the Animal nutrition component of feed, is conducive to amblyseius cucumeris oudeman and quickly breeds.In feed
The middle appropriate agar powder (2%) of addition, is slowed down the evaporation rate of Moisture in Feed with this, has moisture-keeping function concurrently, while can avoid cucumber
It is drowned during amblyseius as predatory mite feeding, is not easy to adhere to young mite, is conducive to Predatory Mites breeding.Therefore, feed dosage form of the invention is glue
Shape feed.
The present invention overcomes amblyseius cucumeris oudeman it is low to the utilization rate of liquid feed containing pollen the problem of, with tussah chrysalis tissue fluid
Pollen is substituted, while man-made feeds holozoic nutrition is improved, increases liquid animal nutrition and the mixing of other components, carries
The utilization rate of high holozoic nutrition component.The gluey artificial modified for amblyseius cucumeris oudeman population indoor propagation of the present invention is raised
Material, low in raw material price, can be effectively performed amblyseius cucumeris oudeman population indoor propagation, to later Amblysiuss class Predatory Mites scale
Metaplasia production has positive facilitation.
Embodiment
With reference to embodiment, the present invention is furture elucidated.
Embodiment 1
Suitable for amblyseius cucumeris oudeman population indoor propagation gluey artificial modified feed formula as shown in table 1 below-table 3.
Tussah chrysalis is bought by market, its extracting method includes step：After removing tussah chrysalis body wall and middle intestines, tussah chrysalis is put
In being fully ground at low temperature in mortar, then by ground pupa tissue fluid be placed in 50ml centrifuge tubes under 6000rmp from
Heart 1min, Aspirate supernatant are placed in 10ml centrifuge tubes, are then placed in 4 DEG C of refrigerators, spare
Including the following raw material：Tussah chrysalis tissue fluid, yeast extraction powder, tryptone, glucose, honey and fresh yolk, respectively
The parts by weight of raw material are respectively：10 parts of tussah chrysalis tissue fluid, 5 parts of powder of yeast extraction, 5 parts of tryptone, 5 parts of glucose, honey 5
10 parts of part and fresh yolk.
2nd, influence of the component of artificial modified feed to amblyseius cucumeris oudeman feeding effect
Contrast test is specific as follows, and as shown in table 1, formula AD1 is tussah chrysalis tissue fluid+matrix, formula AD2 is fresh milk
+ matrix, formula AD3 are rape pollen+matrix, formula AD4 is camellia pollen+matrix, formula AD5 is matrix, wherein, matrix is
+ 10% fresh yolk of+5% honey of 5% yeast extract+5% glucose of+5% tryptone；3.CK is ellipse dispersion.According to institute
Each formula is arranged to weighing appropriate each component, pollen is fully dissolved with suitable distilled water, it is spare；Sucrose is put into the burning of 100mL
In cup, the distilled water needed for proportioning is added, being sufficiently stirred dissolves sucrose, adds appropriate agar powder (the 2% of total amount).Then
Beaker is put into micro-wave oven and heats 10-20s, is completely dissolved agar, is then taken out, treats that solution is cooled to close to room temperature not
During solidification, tussah chrysalis tissue fluid, milk, rape pollen or camellia pollen, and tryptone, yeast extract, bee are separately added into
The man-made feeds made, are placed under the conditions of 4 DEG C are sealed afterwards by honey, fresh yolk, spare.
1 amblyseius cucumeris oudeman man-made feeds formula of table
2 amblyseius cucumeris oudeman man-made feeds formula of table
Under be sealed, it is spare.
3 amblyseius cucumeris oudeman man-made feeds formula of table
Experiment is in 27 ± 1 DEG C of temperature, relative humidity RH70 ± 5% and illumination 16L:In the intelligent growth cabinet of 8D into
OK, using water pond method, one piece of square sponge (11cm × 11cm × 1cm) is placed in square culture dish (13cm × 13cm),
A piece of black PVC plastic tab (11cm × 11cm × 0.1cm) is placed on sponge, suitable distillation is then added into culture dish
Water, makes sponge fully absorb water, and to prevent mite from escaping, and has moisture-keeping function concurrently.
The amblyseius cucumeris oudeman chosen respectively on plastic tab in culture dish into 50 oogenesis is female into mite, makes its oviposition, receives
It is spare to collect produced ovum in 6h.The black plastic thin slice in culture dish is put it to 1 amblyseius cucumeris oudeman ovum of #0 writing brushes picking
Upper (2.5cm × 2.5cm × 0.1cm, every piece of sponge on uniformly place 9), and it is prepared in thin slice center to place a fritter
Feed, is replaced once per 24h；Feeding ellipse dispersion is set for control, each processing retains 15 times and effectively repeats.Observed per 8h
The developmental state of 1 amblyseius cucumeris oudeman, and record, to find that husking is into new mite state as standard, when amblyseius cucumeris oudeman is developed
To into after mite, by hero into mite be incorporated into it is female it is normally mated on plastic tab where mite, it is dead if any male mite during experiment
Die or escape, supplemented in time, record oviposition duration, incubation rate and death condition.Into into the male and female for after mite, counting into mite
Than, and will be matched after husking into mite, during the female oviposition into mite, per 8h, observation once, records egg laying amount, then chooses ovum,
Untill the female death into mite.
All data carry out single sample Kolmogorov- using SPSS20.0 softwares using non-parametric test method
Smirnov is examined, and determines whether data meet normal distribution, and then profit Tukey HSD methods carry out Multiple range test.
The development duration of each mite state is shown in Table 4 after amblyseius cucumeris oudeman feeding difference man-made feeds.The result shows that in temperature 28
DEG C, relative humidity RH70 ± 5%, illumination 16L:Under conditions of 8D, amblyseius cucumeris oudeman feeding can be just after respectively handling man-made feeds
Often it is developed into mite, and is bred.Under test conditions, after amblyseius cucumeris oudeman feeding respectively matches feed, its each phasic development
The phase is gone through, most long with the ovum phase, the young mite puberty is most short, and preceding deutonymph phase and deutonymph phase are close.Amblyseius cucumeris oudeman feeding different feeds
When, the phase is gone through in female mite and male each phasic development of mite does not have significant difference, and in general, male mite each time stage of development is slightly longer than
Female mite.
It is 5.9d the time required to completing one from generation to generation, for everywhere when amblyseius cucumeris oudeman is using ellipse dispersion as predation prey
It is most short in reason, secondly using in matrix add tussah chrysalis tissue fluid for food, completion one generation time be 6.4d, substantially less than with
Other four kinds of feeds are the development duration of food, than the long 0.44d, the two significant difference (F=using ellipse dispersion as predation prey
20.081；Df=5,84；P<0.0001).In prepared man-made feeds and using ellipse dispersion as in the processing eaten, cucumber is blunt
Pacify mite children mite development duration there was no significant difference (F=1.722；Df=5,84；P=0.1384), mainly due to young mite phase feeding
Measure small or not feeding, mobility is weaker, scope of activities is smaller, development duration is short, food it is influenced it is weaker, all processing
Amblyseius cucumeris oudeman children's mite development duration is below 1.0d.
This experiment matches each one work feed and there is influence to amblyseius cucumeris oudeman egg development, is hunted by predation of ellipse dispersion
The amblyseius cucumeris oudeman egg development of thing goes through phase minimum 1.9d, with AD1 (including AD1-1~AD1-3) and AD4 (including AD4-1~
AD4-3) puberty for food takes second place, and mainly due to food nourishment composition, female to amblyseius cucumeris oudeman into mite endotoky, there are shadow
Ring (F=10.736；Df=5,84；P<0.0001).When amblyseius cucumeris oudeman feeding ellipse dispersion and addition tussah chrysalis tissue fluid
Matrix when, corresponding preceding deutonymph development duration is respectively 1.6d and 1.8d, deutonymph development duration be respectively 1.5d and
1.7d, there are significant difference (F=10.075,3.903；Df=5,84；P<0.0001 ,=0.0031).
Development impact (M ± SE) of the different man-made feeds of table 4 to amblyseius cucumeris oudeman
Note：1. institute's column data is average value ± standard error in table；2. different lowercase letter indication difference conspicuousnesses in same column
(Tukey HSD, P<0.05)；3.AD1 is tussah chrysalis tissue fluid+matrix, AD2 is fresh milk+matrix, AD3 is rape pollen+base
Matter, AD4 are camellia pollen+matrix, AD5 is matrix, its mesostroma is+5% glucose+5% of+5% tryptone of 5% yeast extract
The fresh yolk of honey+10%；3.CK is ellipse dispersion.
Amblyseius cucumeris oudeman is euryphagy Predatory Mites, and various plants pollen can alternatively feed, but different phytoseiid mites pair
Nutritional ingredient needed for absorbing requires difference, and to nutriment, there is also different hobbies.Under the conditions of 25 DEG C indoor, cucumber is blunt to pacify
For mite from ovum into when preying on dust mite, Frankliniella occidentalis and onion thrips during mite respectively, its development duration is respectively 7.0,8.7 and
8.2d.In this experiment, when feed is respectively matched in amblyseius cucumeris oudeman feeding, it is below what is reported to the development duration into mite by ovum
Development duration under equal conditions using dust mite, Frankliniella occidentalis and onion thrips as predation prey.Research is found, works as amblyseius cucumeris oudeman
During man-made feeds of the feeding based on tussah chrysalis tissue fluid, its ovum to the development duration ratio into mite is using oval baking soda to prey on prey
Long 0.7d.
Due to this experiment apolegamy feed with it is previously reported different, cause amblyseius cucumeris oudeman ovum extremely into mite development duration
Had differences with previously reported, from experimental result as can be seen that the feed based on tussah chrysalis tissue fluid that this experiment is matched
Nutrition more horn of plenty, suitable for amblyseius cucumeris oudeman population indoor propagation.
The foregoing examples are only illustrative of the present invention, does not form the limitation to protection scope of the present invention, all
It is to be belonged to the same or similar design of the present invention within protection scope of the present invention.
Claims (6)
1. the gluey artificial modified feed of a kind of amblyseius cucumeris oudeman population indoor propagation, it is characterised in that the artificial modified is raised
Material includes matrix and agar powder, wherein the matrix includes：Tussah chrysalis tissue fluid, yeast extraction powder, tryptone, glucose,
Honey and fresh yolk, the weight of each base starting material are：10-15 parts of tussah chrysalis tissue fluid, 5-10 parts of powder of yeast extraction, pancreas
30-65 parts of 5-10 parts of peptone, 5-10 parts of glucose, 5-10 parts of honey, 10-15 parts of fresh yolk and distilled water.
2. the gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation according to claim 1, it is characterised in that
The matrix includes：It is 10 parts of tussah chrysalis tissue fluid, 5 parts of powder of yeast extraction, 5 parts of tryptone, 5 parts of glucose, 5 parts of honey, fresh
60 parts of 10 parts of yolk and water.
3. the gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation according to claim 2, it is characterised in that
The artificial modified feed includes the agar of gross weight 2%.
4. the gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation according to claim 1, it is characterised in that
The tussah chrysalis tissue fluid is prepared by method comprising the following steps：
(1) after removing tussah chrysalis body wall and middle intestines, tussah chrysalis is ground at low temperature；
(2) and then by ground pupa tissue fluid centrifuge, Aspirate supernatant obtains tussah chrysalis tissue fluid.
5. the gluey artificial modified feed of amblyseius cucumeris oudeman population indoor propagation according to claim 4, it is characterised in that
In step (2), ground pupa tissue fluid is centrifuged into 1min under 6000rmp.
A kind of 6. method of artificial propagation amblyseius cucumeris oudeman, it is characterised in that the described method includes usage right to require 1 Hu
The step of melon amblyseius as predatory mite population indoor propagation is with gluey artificial modified feed feeding amblyseius cucumeris oudeman.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711272615.XA CN107927356B (en) | 2017-12-06 | 2017-12-06 | Colloidal artificial improved feed for indoor propagation of amblyseius cucumeris population |
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| CN201711272615.XA CN107927356B (en) | 2017-12-06 | 2017-12-06 | Colloidal artificial improved feed for indoor propagation of amblyseius cucumeris population |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103271247A (en) * | 2013-06-03 | 2013-09-04 | 浙江省农业科学院 | Artificial feed of cyrtorhinus lividipennis |
| CN103734089A (en) * | 2013-12-26 | 2014-04-23 | 中山大学 | Artificial breeding method of predatory mites of phytoseiidae |
| CN103750016A (en) * | 2013-12-26 | 2014-04-30 | 中山大学 | Liquid feed used for feeding phytoseiidae predatory mites and preparation method of liquid feed |
| CN104642792A (en) * | 2015-01-27 | 2015-05-27 | 湖南省烟草公司长沙市公司 | Universal artificial feed for ladybird and preparation and application methods of universal artificial feed |
| CN105851720A (en) * | 2016-04-27 | 2016-08-17 | 浙江省农业科学院 | Artificial feed for tytthus chinensis |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103271247A (en) * | 2013-06-03 | 2013-09-04 | 浙江省农业科学院 | Artificial feed of cyrtorhinus lividipennis |
| CN103734089A (en) * | 2013-12-26 | 2014-04-23 | 中山大学 | Artificial breeding method of predatory mites of phytoseiidae |
| CN103750016A (en) * | 2013-12-26 | 2014-04-30 | 中山大学 | Liquid feed used for feeding phytoseiidae predatory mites and preparation method of liquid feed |
| CN104642792A (en) * | 2015-01-27 | 2015-05-27 | 湖南省烟草公司长沙市公司 | Universal artificial feed for ladybird and preparation and application methods of universal artificial feed |
| CN105851720A (en) * | 2016-04-27 | 2016-08-17 | 浙江省农业科学院 | Artificial feed for tytthus chinensis |
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