CN106942157B - A kind of Moringa Nao snout moth's larva method for breeding using man-made feeds - Google Patents
A kind of Moringa Nao snout moth's larva method for breeding using man-made feeds Download PDFInfo
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- CN106942157B CN106942157B CN201710125324.1A CN201710125324A CN106942157B CN 106942157 B CN106942157 B CN 106942157B CN 201710125324 A CN201710125324 A CN 201710125324A CN 106942157 B CN106942157 B CN 106942157B
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- 210000004894 snout Anatomy 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000009395 breeding Methods 0.000 title claims abstract description 5
- 230000001488 breeding effect Effects 0.000 title claims abstract description 5
- 241000220215 Moringa Species 0.000 claims abstract description 19
- 235000011347 Moringa oleifera Nutrition 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 11
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 11
- 210000004681 ovum Anatomy 0.000 claims abstract description 11
- 229920001817 Agar Polymers 0.000 claims abstract description 10
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 10
- 239000008272 agar Substances 0.000 claims abstract description 10
- 235000013601 eggs Nutrition 0.000 claims abstract description 9
- 241000382353 Pupa Species 0.000 claims abstract description 7
- 241000238631 Hexapoda Species 0.000 claims abstract description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 6
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims abstract description 6
- 235000010199 sorbic acid Nutrition 0.000 claims abstract description 6
- 239000004334 sorbic acid Substances 0.000 claims abstract description 6
- 229940075582 sorbic acid Drugs 0.000 claims abstract description 6
- 230000032669 eclosion Effects 0.000 claims abstract description 5
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 4
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 4
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 4
- 125000004383 glucosinolate group Chemical group 0.000 claims abstract description 4
- 239000002245 particle Substances 0.000 claims abstract description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 12
- 230000017448 oviposition Effects 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000013589 supplement Substances 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 5
- 230000001419 dependent effect Effects 0.000 claims description 3
- 230000019617 pupation Effects 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 2
- 239000005394 sealing glass Substances 0.000 claims description 2
- 238000011161 development Methods 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 abstract description 4
- 238000004321 preservation Methods 0.000 abstract description 4
- 239000012141 concentrate Substances 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 235000019629 palatability Nutrition 0.000 abstract description 2
- 238000012546 transfer Methods 0.000 abstract description 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 abstract 1
- 239000011521 glass Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241001580122 Noorda blitealis Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
A kind of Moringa Nao snout moth's larva method for breeding using man-made feeds belongs to technical field of insect feeding.The formula of man-made feeds are as follows: Moringa 99~101g of leaf dried powder, 9~11g of agar, 4.5~5.5g of yeast powder, 0.095~1.05ml of formaldehyde, 0.45~0.55g of sorbic acid, 0.38~0.42g of ascorbic acid, the particle that diameter is 0.4~0.6cm is made in 19~21mg of glucosinolates, water 500ml after mixing.Larva goes through the phase 8~11 days, and pupa time 5~9 days, the adult stage 5~7 days, specific steps included: the acquisition in Moringa Nao snout moth's larva source, the preservation of pupa and adult eclosion, the preservation of Adult worms producting eggs and ovum, raising and transfer of larva etc..Man-made feeds are easy to save, low in cost, and the palatability of Moringa Nao snout moth's larva is good, and polypide development is normal, such that high density concentrates raising Moringa Nao snout moth's larva, provide experiment worm sources to stablize.
Description
Technical field
The invention belongs to technical field of insect feeding, specifically a kind of Moringa Nao snout moth's larva raising side using man-made feeds
Method.
Background technique
Moringa complete stool various pieces can develop and use and widely used, such as ornamental, eat, medicinal, for planting
Purification and the ideal oilseed plant of object oil and beer, nectariferous plant, herding material and yule logs.Moringa Nao snout moth's larva (Noorda
Blitealis it is) newfound a kind of Microlepidoptera pest on China's Moringa, is mainly caused harm with larval feeding blade, harm is tight
It can be by Moringa blade eat everything up when weight.Existing Moringa Nao snout moth's larva distribution is saved in Yunnan Province of China at present, in some Moringa gardens
Population outbreak, and have the tendency that gradually extending, seriously threaten Moringa production.For safe prevention Moringa Nao snout moth's larva, should reinforce pair
The biology and Study of Prevention Technology of Moringa Nao snout moth's larva, this just needs artificial feeding Moringa Nao snout moth's larva, establishes stable indoor population, solution
Certainly test the supply problem of worm sources.
Once there were the report with fresh Moringa blade raising Moringa Nao snout moth's larva in foreign countries, but there are distinct disadvantages for this method: peppery
The wooden blade holding time is short, and humidity is then easy to rot greatly, and humidity is low to be then easy to dry up, and influences Moringa Nao snout moth's larva larval feeding；
In addition, Moringa Nao snout moth's larva larva is very big in gluttony phase appetite, largely it must feed and clear up daily using the raising of fresh Moringa blade, consume
Take a large amount of manpower and material resources.
Summary of the invention
The purpose of the present invention is to overcome the shortcomings of the existing technology, provides a kind of Moringa Nao snout moth's larva raising using man-made feeds
Method, to use manpower and material resources sparingly, solve selected insect source supply problem, it is established that the Moringa Nao snout moth's larva method for breeding of indoor population.
The method of the present invention is as follows:
1,3 more than age healthy Moringa Nao snout moth's larva larvas are collected from field, 29~31 DEG C, relative humidity 70% ± 10%,
Under conditions of illumination L:D=14 ︰ 10, raised with man-made feeds to pupating；
2, healthy person is put into dependent insect cage in step gained Moringa Nao snout moth's larva pupa in selection, in 29~31 DEG C, relative humidity 70%
Under conditions of ± 10% shading save, be put into after adult eclosion, in cage weight concentration be 4.5~5.5% hydromel at
Worm supplements the nutrients；
3, under conditions of 29~31 DEG C, relative humidity 70% ± 10% and shading, by adult obtained by upper step according to male and female
Quantity is placed in the bottle of gauze sealing than the ratio for 1:2, be put into bottle hydromel that weight concentration is 4.5~5.5% at
Worm supplements the nutrients, and bottle bottom places oviposition paper, lays eggs for female adult, checks oviposition situation, after 2~3 days, pieces of an egg blackening daily
After can carry out next step raising；
4, under conditions of 29~31 DEG C, relative humidity 70% ± 10%, illumination L:D=14 ︰ 10, by the ovum of upper step blackening
Block is placed in the bottle equipped with fresh Moringa blade and is sealed with gauze, culture 6~12 hours i.e. visible larvae hatch, ovum and new
Fresh Moringa blade quantity ratio is that every ovum places 1~2 blade；
5, the larva access that 1~2 age in days of upper step hatches is placed in the breathable sealing glass container of man-made feeds, often
The feed of replacement in 3~4 days, until larvae pupation, the feed relative being put into every time is 2~3 times of Weight of larvae；
6, continuously rearing: repeat according to above 2 to 5 step；
The formula of described man-made feeds are as follows: Moringa 99~101g of leaf dried powder, 9~11g of agar, 4.5~5.5g of yeast powder, first
0.095~1.05ml of aldehyde, 0.45~0.55g of sorbic acid, 0.38~0.42g of ascorbic acid, 19~21mg of glucosinolates, water
The particle that diameter is 0.4~0.6cm is made in 500ml after mixing.
Man-made feeds can be prepared as follows:
Load weighted Moringa leaf dried powder and yeast powder are first added to the cold water of formula total Water 3/5, stir evenly be made into it is outstanding
Supernatant liquid, it is spare；
Load weighted agar powder is poured into pot again, the water of formula total Water 2/5 is added, be heated to boiling and constantly uses glass
The stirring of glass stick；
Sorbic acid is added under fluidized state, mixes thoroughly, stops heating, formaldehyde is added, agar solution is made；
The suspension is poured into the agar solution cool to 60~65 DEG C, adds load weighted ascorbic acid powder,
It stirs evenly cooled and solidified and obtains man-made feeds.
It is an advantage of the invention that man-made feeds are easy to save, low in cost, the palatability of Moringa Nao snout moth's larva is good, polypide development
Normally, make it possible that high density concentrates raising Moringa Nao snout moth's larva, provide experiment worm sources to stablize.
Specific embodiment
See following embodiment:
Larva goes through the phase 8~11 days, and pupa time 5~9 days, the adult stage 5~7 days, specific steps are as follows:
(1) acquisition in Moringa Nao snout moth's larva source
From the healthy larva more than age of field acquisition Moringa Nao snout moth's larva 3, in 30 DEG C, relative humidity 70% ± 10%, illumination item
Under conditions of part L:D=14 ︰ 10, raised with man-made feeds to pupating.
(2) preservation of pupa and adult eclosion
The Moringa Nao snout moth's larva pupa of health is collected and is put into dependent insect cage, in 30 DEG C, the item of relative humidity 70% ± 10%
Shading saves under part, is put into the hydromel that weight concentration is 5% after adult eclosion, in cage for Adult supplement nutrient.
(3) preservation of Adult worms producting eggs and ovum
Under conditions of 30 DEG C, relative humidity 70% ± 10% under dark conditions, adult is the number of 1:2 according to sex ration
Amount, is placed in oblate glass candy bottle, bottleneck is sealed with gauze, prevents adult from escaping.It is 5% that weight concentration is put into bottle
Hydromel for Adult supplement nutrient appearance, bottle bottom place two by commercially available 80g printing paper fan-folded (radius
10.5cm, wide 2cm) oviposition paper, for female adult oviposition.Daily regular check oviposition situation, and the paper of replacement oviposition in time.
Daily with a batch of ovum, can be put together at one, after 2~3 days, pieces of an egg be slightly variable it is black after can be transferred to can
In bottle.
(4) raising and transfer of larva
Under conditions of 30 DEG C, relative humidity 70% ± 10%, 10 ︰ illumination condition L:D=14, the pieces of an egg of blackening are placed
It in the can equipped with fresh Moringa blade (bore 7cm, high 10cm), and is sealed with gauze, prevents larva from escaping.Every bottle puts
80 ovum are set, ovum and fresh Moringa blade quantity ratio are that every ovum places 2 blades；Cultivate 8 hours visible larvae hatch；
1~2 day old larva is gently accessed in the finger-type pipe for being placed with man-made feeds with writing brush (bore 2cm, high 7cm), and
It is sealed with tampon.Every pipe places 5 larvas, 3 feeds.The feed of replacement in every 3 days, until larvae pupation.It is put into every time
Feed relative is 2 times of Weight of larvae.
(5) continuously rearing
Repeat according to the step of (2) to (4).
The formula of man-made feeds used are as follows: Moringa leaf dried powder 100g, agar 10g, yeast powder 5g, formaldehyde 1ml, sorbic acid
The particle of diameter 0.5cm is made in 5g, ascorbic acid 0.4g, glucosinolates 20mg, water 500ml after mixing.
Man-made feeds are prepared as follows:
Load weighted Moringa leaf dried powder and yeast powder are first added to the cold water of formula total Water 3/5, stir evenly be made into it is outstanding
Supernatant liquid, it is spare；
Load weighted agar powder is poured into pot again, the water of formula total Water 2/5 is added, be heated to boiling and constantly uses glass
The stirring of glass stick；
Sorbic acid is added under fluidized state, mixes thoroughly, stops heating, formaldehyde is added, agar solution is made；
The suspension is poured into the agar solution cool to 65 DEG C, adds load weighted ascorbic acid powder, is stirred
Uniform cooled and solidified obtains man-made feeds.
Relevant comparative's experimental data of 1 feed of embodiment
Influence of the 1 two kinds of feeds of table to Moringa Nao snout moth's larva development duration
Note: data listed by table 1 are average ± standard error, identical according to t inspection result with alphabet registration in a line
Letter indicates 0.05 level without significant difference.
Above data show embodiment feed can under Enhancement test room environmental artificial feeding Moringa Nao snout moth's larva indices.
Influence of the 2 two kinds of feeds of table to Moringa Nao snout moth's larva growth and development
Note: data listed by table 2 are average ± standard error, and alphabet registration is identical according to t inspection result in same row
Letter indicates 0.05 level without significant difference.
Above data shows embodiment feed and can steadily cultivate Moringa Nao snout moth's larva in laboratory environments.
Claims (1)
1. a kind of Moringa Nao snout moth's larva method for breeding using man-made feeds, it is characterised in that as follows:
(1), 3 healthy Moringa Nao snout moth's larva larva more than age is collected from field, in 29~31 DEG C, relative humidity 70% ± 10%, light
Under conditions of L:D=14 ︰ 10, raised with man-made feeds to pupating；
(2), in selection step gained Moringa Nao snout moth's larva pupa in healthy person be put into dependent insect cage, 29~31 DEG C, relative humidity 70% ±
Shading saves under conditions of 10%, and the hydromel that weight concentration is 4.5~5.5% is put into after adult eclosion, in cage for adult
It supplements the nutrients；
(3), under conditions of 29~31 DEG C, relative humidity 70% ± 10% and shading, by adult obtained by upper step according to male and female number
Amount is placed in the bottle of gauze sealing than the ratio for being 1:2, and the hydromel that weight concentration is 4.5~5.5% is put into bottle for adult
It supplements the nutrients, bottle bottom places oviposition paper, lays eggs for female adult, oviposition situation is checked daily, after 2~3 days, after pieces of an egg blackening
It can carry out next step raising；
(4), under conditions of 29~31 DEG C, relative humidity 70% ± 10%, illumination L:D=14 ︰ 10, by the pieces of an egg of upper step blackening
Be placed in the bottle equipped with fresh Moringa blade and sealed with gauze, culture 6~12 hours i.e. visible larvae hatch, ovum with it is fresh
Moringa blade quantity ratio is that every ovum places 1~2 blade；
(5), the larva access that 1~2 age in days of upper step hatches is placed in the breathable sealing glass container of man-made feeds, every 3
The feed of replacement in~4 days, until larvae pupation, the feed relative being put into every time is 2~3 times of Weight of larvae；
(6), continuously rearing: repeat according to above 2 to 5 step；
The formula of described man-made feeds are as follows: Moringa 99~101g of leaf dried powder, 9~11g of agar, 4.5~5.5g of yeast powder, formaldehyde
0.095~1.05ml, 0.45~0.55g of sorbic acid, 0.38~0.42g of ascorbic acid, 19~21mg of glucosinolates, water 500ml,
The particle that diameter is 0.4~0.6cm is made after mixing.
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| CN108496900B (en) * | 2018-04-24 | 2020-11-24 | 云南农业大学 | Breeding method and using method for preventing and treating lygus tridentatus for moringa oleifera naotus |
| CN111264476B (en) * | 2020-03-12 | 2024-06-07 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg block collecting method and device |
| CN111838457B (en) * | 2020-07-22 | 2023-05-05 | 广州岭南穗粮谷物股份有限公司 | Preparation method of larva block feed for cnaphalocrocis medinalis and cultivation and throwing method of moth cocoon bees |
| CN114223655B (en) * | 2021-12-23 | 2023-05-02 | 西南林业大学 | Moringa oleifera agate and borer attractant based on moringa oleifera leaf volatile substances |
| CN117941655B (en) * | 2024-03-13 | 2024-05-31 | 暨南大学 | Efficient breeding method for culex pallidum |
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| CN101507472B (en) * | 2009-03-19 | 2011-09-07 | 中国农业科学院植物保护研究所 | Artificial feedstuff of striped rice borer, preparation method thereof and breeding method |
| CN101971931B (en) * | 2010-11-09 | 2012-07-04 | 中国农业科学院植物保护研究所 | Artificial feed of sesamia inferens walker as well as preparation method and feeding method thereof |
| CN103918937B (en) * | 2014-04-28 | 2016-01-20 | 广西壮族自治区蚕业技术推广总站 | Castor silkworm man-made feeds |
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