CN103844142A - Improved artificial diet of prodenia litura larvae and preparation method thereof - Google Patents
Improved artificial diet of prodenia litura larvae and preparation method thereof Download PDFInfo
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- CN103844142A CN103844142A CN201410036783.9A CN201410036783A CN103844142A CN 103844142 A CN103844142 A CN 103844142A CN 201410036783 A CN201410036783 A CN 201410036783A CN 103844142 A CN103844142 A CN 103844142A
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Abstract
The invention discloses an improved artificial diet of prodenia litura larvae and a preparation method of the improved artificial diet. The improved artificial diet is prepared from the following raw materials: 200g of corn flour, 100g of soybean flour, 12g of yeast powder, 6g of ascorbic acid, 2g of sorbic acid, 2g of methylparaben, 15g of agar, 15ml of compound vitamin liquid, 50g of cane sugar, and 1000g of water. The preparation method comprises the following steps: uniformly mixing the corn flour, the soybean flour and the yeast powder, and steaming a mixture in a pot for 30 minutes for later use; boiling the agar and the cane sugar with distilled water till the agar and the cane sugar are completely dissolved, cooling the solution to about 50 DEG C, adding the sorbic acid, the methylparaben, the ascorbic acid and the compound vitamin liquid, and stirring till the materials are dissolved; and adding the steamed mixture of the corn flour, the soybean flour and the yeast powder into the solution, stirring uniformly, pouring into a sterilized preservation box, cooling to room temperature, and sealing and refrigerating in a refrigerator at 4 DEG C, thereby obtaining the improved artificial diet ready to use as required. The prodenia litura can be bred indoors in a subculture manner with the improved artificial diet, thereby obtaining a large quantity of prodenia litura sources with identical growth.
Description
Technical field
The present invention relates to a kind of man-made feeds and preparation method thereof, particularly man-made feeds of a kind of improved Spodoptera litura larvae and preparation method thereof.
Background technology
Prodenia litura Prodenia litura(Fabricius) belong to Lepidoptera (Lepidoptera), Noctuidae (Noctuidae), is global polyphagy, migratory pest.Mainly cause harm with larva, after 4 ages in days, enter the gluttony phase, be injured plant gently blade eaten into incise, hole or netted, heavy surplus vein only, more severe patient even endangers cane, causes total crop failure, and the worm excrement of discharge pollutes melon dish, insect bite wound easily brings out the diseases such as the soft corruption of fruits and vegetables, epidemic disease, virosis, has a strong impact on the quality and yield of crop.In recent years, along with the variation of China's crop mix, add climate warming, harm occurs prodenia litura increases the weight of year by year, rises to rapidly the normal property sent out, fulminant agriculture major pest by the intermittence in past, sporadic, less important property insect.
For effectively controlling the harm of prodenia litura, the expansion such as biology, ecology and Efficacy of Pesticide to prodenia litura further investigation is a basic job, particularly prevents and treats in the work such as screening, the toxicologic study of pharmacy effect of prodenia litura effective agent and must have raised growth to grow consistent examination worm.Given this, how to raise a large amount of examination of acquisition worms by man-made feeds and just become one of important step of prodenia litura research.
About the research of prodenia litura man-made feeds, Pu Tiansheng etc. (1980) and Lu Wenhua etc. (1986) have reported a kind of prodenia litura semi-synthetic diet, but this feed depends on prodenia litura natural host, as cassava leaf meal and castor leaf powder.Chen Qijin etc. (2000) have reported the simple and easy feed of five kinds of noctuids of a kind of raising, but feeding effect is very undesirable.Zhu Limei etc. (2000), Huang Yanjun and Pu hat diligent (2011) have been reported a kind of man-made feeds of raising prodenia litura, but all without this composition of sucrose, it is not fine raising result.And be coated with industry illicit sexual relations Zeng Jihua (2010) report about having added potassium hydroxide, acetic acid and formaldehyde etc. in prodenia litura man-made feeds, preparation feed excitant is out large, insect eating effect is very undesirable.Lv Baoqian etc. (2013) publish about in the patent of prodenia litura man-made feeds containing any vitamin, its nutritive value is not high, feeding effect is bad.
Summary of the invention
The defect or the deficiency that exist for above-mentioned prior art, the object of the invention is to, for vegetable-crop pest-insect---and the needs of the indoor mass rearing of prodenia litura, screen man-made feeds formula of a kind of improved applicable mass rearing prodenia litura and preparation method thereof.
In order to realize above-mentioned task, the present invention takes following technical solution:
A kind of man-made feeds of Spodoptera litura larvae, it is characterized in that, these man-made feeds that make are made up of the raw material of following ingredients and weight: corn flour: 200g, soy meal: 100g, dusty yeast: 12g, ascorbic acid: 6g, sorbic acid: 2g, methyl hydroxybenzoate: 2g, agar: 15g, composite vitamin liquid 15ml, sucrose 50g, water 1000g.
Described composite vitamin liquid is made up of the raw material of following ingredients and weight: nicotinic acid (V
b5): 1.528g, D-VB5 calcium (V
b3): 1.525g, riboflavin: 0.764g, vitamin B1: 0.382g, vitamin B6: 0.382g, folic acid: 0.382g, Bio: 0.305g, cyanocobalamin: 0.003g, water: 500ml.
The preparation method of above-mentioned Spodoptera litura larvae man-made feeds, is characterized in that, comprises the following steps:
A, first by formula composite vitamin liquid is configured, put 4 ℃ of refrigerators for subsequent use;
B, weigh corn flour, soy meal, dusty yeast, ascorbic acid, sorbic acid, methyl hydroxybenzoate, agar, sucrose and distilled water by formula;
C, put into pot after corn flour, soy meal, dusty yeast are mixed and steam after 30min stand-by;
D, agar and sucrose adding distil water are boiled until dissolve completely, while being cooled to 50 ℃ of left and right, add sorbic acid, methyl hydroxybenzoate, ascorbic acid and composite vitamin liquid, stir until dissolve, then add therein the mixture of steamed corn flour, soy meal and dusty yeast, after stirring, pour in the plastic preserving box of having sterilized, to be cooled to room temperature, sealing, be placed in 4 ℃ of refrigerations of refrigerator, ready access upon use.
Spodoptera litura larvae man-made feeds of the present invention, the each raw material of its prescription is easy to buy, and compound method is simple, can raise prodenia litura at indoor subculture, obtains raised growth and grows consistent worm source.
The specific embodiment
In order clearly to understand the present invention, below in conjunction with embodiment, the present invention is described in further detail.
Embodiment:
The man-made feeds of Spodoptera litura larvae prepared by the present embodiment, it is filled a prescription referring to table 1.
Table 1: improved Spodoptera litura larvae man-made feeds formula
| Composition (Ingredient) | Consumption (Dosage) (g) | Source (Source) |
| Corn flour (Maize powder) | 200 | Commercial |
| Soy meal (Soybean powder) | 100 | Commercial |
| Dusty yeast (Yeast powder) | 12 | Angel Yeast Co.,Ltd |
| Ascorbic acid L-ascorbic acid | 6 | Xi'an chemical reagent factory |
| Sorbic acid (Sorbic acid) | 2 | Tianjin Bo Di Chemical Co., Ltd. |
| Methyl hydroxybenzoate (Nipagin ester) | 2 | Tianjin Deng Feng chemical reagent factory |
| Agar (Agar) | 15 | The south of Fujian Province agar-agar Co., Ltd |
| Distilled water (De-ionized water) | 1000 | Laboratory's self-control |
| Composite vitamin liquid (Vitamins liquid) | 15ml | Laboratory's autogamy |
| Sucrose (Saccharose) | 50g | Commercial |
Concrete preparation follows these steps to carry out:
1, by the composite vitamin liquid of formulated, its compound method is in every 500ml water, to add following raw material:
Nicotinic acid (V
b5): 1.528g, D-VB5 calcium (V
b3): 1.525g, riboflavin: 0.764g, vitamin: B1(V
b1): 0.382g, vitamin B6 (V
b6): 0.382g, folic acid 0.382g, Bio 0.305g, cyanocobalamin: 0.003g, manual gently stirring is put into 4 ℃ of refrigerators for subsequent use after each raw material dissolves;
2, take 200g corn flour, 100g soy meal, 12g dusty yeast, 6g ascorbic acid, 2g sorbic acid, 2g methyl hydroxybenzoate, 15g agar, 50g sucrose and 1000ml distilled water by formula;
3, putting into pot after 200g corn flour, 100g soy meal and 12g dusty yeast are mixed steams after 30min stand-by;
4,15g agar and 50g sucrose being added to 1000ml distilled water boils until dissolve completely, while being cooled to 50 ℃ of left and right, add the composite vitamin liquid of 2g sorbic acid, 2g methyl hydroxybenzoate, 6g ascorbic acid and 15ml, stir until dissolve, then add therein the mixture of steamed corn flour, soy meal and dusty yeast, after stirring, pour crisper (the diameter 18cm after the clorox soaking disinfection 12h that has been 0.2% by concentration into, height 10cm) in, to be cooledly to room temperature, seal, be placed in 4 ℃ of refrigerations of refrigerator, ready access upon use.
The evaluation of feeding effect:
The Spodoptera litura larvae man-made feeds of preparing with above embodiment, raise Spodoptera litura larvae, are: temperature in indoor raising condition: 27 ℃ ± 0.5 ℃, and relative humidity: 70 ± 10%, the photoperiod: 16:8(L:D) h, intensity of illumination: 8250lux.
Feeding effect: Spodoptera litura larvae is gone through the phase (14.69 ± 0.3d); Pupa time (9.06 ± 0.03d); Larval phase survival rate average out to 96.28%; Pupa eclosion rate average out to 92.86%; Female and male ratio (0.91 ± 0.006); Single female egg laying amount on average reaches 895.
Than existing man-made feeds (Chen Qijin etc., 2000; Zhu Limei etc., 2000; Huang Yanjun and Pu hat are diligent, and 2011; Be coated with industry illicit sexual relations Zeng Jihua, 2010; Lv Baoqian etc., 2013), Spodoptera litura larvae man-made feeds of the present invention feeding effect the best to Spodoptera litura larvae, and have the advantages such as composition is comprehensive, manufacturing process is relatively simple.
Claims (5)
1. the man-made feeds of an improved Spodoptera litura larvae, it is characterized in that, these man-made feeds that make are made up of the raw material of following ingredients and weight: corn flour: 200g, soy meal: 100g, dusty yeast: 12g, ascorbic acid: 6g, sorbic acid: 2g, methyl hydroxybenzoate: 2g, agar: 15g, composite vitamin liquid: 15ml, sucrose: 50g, water: 1000g.
2. Spodoptera litura larvae man-made feeds as claimed in claim 1, is characterized in that, described composite vitamin liquid is made up of the raw material of following ingredients and weight: nicotinic acid (V
b5): 1.528g, D-VB5 calcium (V
b3): 1.525g, riboflavin: 0.764g, vitamin B1: 0.382g, vitamin B6: 0.382g, folic acid: 0.382g, Bio: 0.305g, cyanocobalamin: 0.003g, water: 500ml.
3. the preparation method of Spodoptera litura larvae man-made feeds claimed in claim 1, is characterized in that, comprises the following steps:
A, first by formula composite vitamin liquid is configured, put 4 ℃ of refrigerators for subsequent use;
B, weigh corn flour, soy meal, dusty yeast, ascorbic acid, sorbic acid, methyl hydroxybenzoate, agar, sucrose and distilled water by formula;
C, put into pot after corn flour, soy meal, dusty yeast are mixed and steam after 30min stand-by;
D, agar and sucrose adding distil water are boiled until dissolve completely, be cooled to 50 ℃, add sorbic acid, methyl hydroxybenzoate, ascorbic acid and composite vitamin liquid, stir until dissolve, then add therein the mixture of steamed corn flour, soy meal and dusty yeast, after stirring, pour in the plastic preserving box of having sterilized, to be cooled to room temperature, sealing, is placed in 4 ℃ of refrigerations of refrigerator, ready access upon use.
4. method as claimed in claim 3, is characterized in that, the compound method of described composite vitamin liquid is: in every 500ml water, add following raw material to mix:
Nicotinic acid (V
b5): 1.528g, D-VB5 calcium (V
b3): 1.525g, riboflavin: 0.764g, vitamin B1: 0.382g, vitamin B6: 0.382g, folic acid: 0.382g, Bio: 0.305g, cyanocobalamin: 0.003g.
5. method as claimed in claim 3, is characterized in that, the disinfectant of described plastic preserving box adopts the clorox that concentration is 0.2%.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957380A (en) * | 2014-12-12 | 2015-10-07 | 昆明学院 | Semi-artificial feed for indoor breeding of Sclerogenia jessica and preparation method thereof |
| CN105124220A (en) * | 2015-08-21 | 2015-12-09 | 福建省农业科学院植物保护研究所 | Semi-synthetic artificial feed for mango horizontal tail noctuid larva and preparation method thereof |
| CN108077191A (en) * | 2017-12-29 | 2018-05-29 | 四川省农业科学院植物保护研究所 | A kind of Spodoptera litura larvae group breeding method |
| CN110583864A (en) * | 2019-10-29 | 2019-12-20 | 玉溪师范学院 | Spodoptera litura artificial feed containing traditional Chinese medicine components and preparation method thereof |
| CN113100358A (en) * | 2021-05-25 | 2021-07-13 | 福建农林大学 | Artificial feed for promoting growth of lepidoptera insects |
| CN115176944A (en) * | 2022-07-29 | 2022-10-14 | 长江大学 | Spodoptera litura artificial feed added with calcium and magnesium elements and preparation method thereof |
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| CN101731482A (en) * | 2010-02-08 | 2010-06-16 | 西北农林科技大学 | Artificial feed for Grapholitha molesta Busck larvae and preparation method thereof |
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| CN101731482A (en) * | 2010-02-08 | 2010-06-16 | 西北农林科技大学 | Artificial feed for Grapholitha molesta Busck larvae and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957380A (en) * | 2014-12-12 | 2015-10-07 | 昆明学院 | Semi-artificial feed for indoor breeding of Sclerogenia jessica and preparation method thereof |
| CN105124220A (en) * | 2015-08-21 | 2015-12-09 | 福建省农业科学院植物保护研究所 | Semi-synthetic artificial feed for mango horizontal tail noctuid larva and preparation method thereof |
| CN105124220B (en) * | 2015-08-21 | 2020-01-17 | 福建省农业科学院植物保护研究所 | Semi-synthetic artificial feed for mango horizontal line tail noctuid larvae and preparation method thereof |
| CN108077191A (en) * | 2017-12-29 | 2018-05-29 | 四川省农业科学院植物保护研究所 | A kind of Spodoptera litura larvae group breeding method |
| CN110583864A (en) * | 2019-10-29 | 2019-12-20 | 玉溪师范学院 | Spodoptera litura artificial feed containing traditional Chinese medicine components and preparation method thereof |
| CN113100358A (en) * | 2021-05-25 | 2021-07-13 | 福建农林大学 | Artificial feed for promoting growth of lepidoptera insects |
| CN115176944A (en) * | 2022-07-29 | 2022-10-14 | 长江大学 | Spodoptera litura artificial feed added with calcium and magnesium elements and preparation method thereof |
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Inventor after: Wu Junxiang Inventor after: Cheng Julong Inventor after: Zhang Nan Inventor after: Xu Xiangli Inventor after: Li Yiping Inventor before: Zhang Nan Inventor before: Wu Junxiang Inventor before: Xu Xiangli Inventor before: Li Yiping |
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Free format text: CORRECT: INVENTOR; FROM: ZHANG NAN WU JUNXIANG XU XIANGLI LI YIPING TO: WU JUNXIANG CHENG JULONG ZHANG NAN XU XIANGLI LI YIPING |
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Effective date of registration: 20141218 Address after: 712100 Shaanxi Province, Xi'an city Yangling District Tai Road No. 3 demonstration Applicant after: Northwest A & F University Applicant after: Shaanxi Tobacco Institute Address before: 712100 Shaanxi Province, Xi'an city Yangling District Tai Road No. 3 demonstration Applicant before: Northwest A & F University |
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Application publication date: 20140611 |