CN104187091A - Eel composite Chinese herbal medicine additive capable of resisting diseases and promoting growth - Google Patents
Eel composite Chinese herbal medicine additive capable of resisting diseases and promoting growth Download PDFInfo
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- CN104187091A CN104187091A CN201410393424.9A CN201410393424A CN104187091A CN 104187091 A CN104187091 A CN 104187091A CN 201410393424 A CN201410393424 A CN 201410393424A CN 104187091 A CN104187091 A CN 104187091A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention discloses an eel composite Chinese herbal medicine additive capable of resisting diseases and promoting growth. The eel composite Chinese herbal medicine additive is prepared by putting all raw materials, such as hairyvein agrimonia herb, radix codonopsis, cochinchinese asparagus root, radix ophiopogonis, rhubarb, radix isatidis, honeysuckle, folium artemisiae argyi, cortex eucommiae, radix astragali, medicated leaven and fructus aurantii, which have the same amount, into a constant-temperature drying oven at the temperature of 60 DEG C until the materials are completely dried, smashing the raw materials by an ultrafine smashing machine, screening the smashed materials by a screen being 120 meshes, and putting the obtained powder into a uniform mixing machine for full mixing. The additive is added into a basic feed for feeding eels, so that the effects of resisting diseases and promoting growth during European eel breeding is achieved; the eel composite Chinese herbal medicine additive has the effects of improving the growth performance of the European eels, increasing the course protein content of body components and increasing intestinal digestive enzyme and immune serum enzyme.
Description
Technical field
The present invention relates to field of fodder, relate in particular to a kind of common eel compound Chinese herbal medicine additive of disease-resistant growth-promoting.
Background technology
European eel delicious meat, nutritious, have clearing heat and detoxicating, eliminate blood stasis and promote tissue regeneration, nourishing such as takes good care of at the multiple efficacies, economic worth is high, is the famous and precious fish of domestic and international one salable.Along with the popularization of intensive culture scale, China supports eel industry and obtains fast development, but cultivation density is blindly improved in some areas, thinks little of environmental health cultivation, and quality safety management exists defect, causes eel disease again and again to break out.Although various fishing medicine antibiotic also can play the positive role that improves survival rate, reduces the loss, bring the problems such as the resistance to the action of a drug, environmental pollution, medicament residue, its application is restricted.Nowadays, fundamentally can improve fish autoimmunity in the urgent need to seeking a kind of new way, alleviation stress, reduce disease rapidly, can reach the object of volume increase simultaneously.
Chinese herb feed additive is formulated according to the theoretical physiological requirements different from animal of Chinese veterinarian by the Chinese herbal medicine of various flavor function.Its active ingredient mainly contains: polysaccharide, alkaloid, protein, amino acid, glucoside, grease, wax, resinae, phytochrome, mineral matter element and vitamin etc.These material effects are coordinated mutually, produce multi-faceted effect, and not only nutritious health-care effect, and directly sterilization, antibacterial regulate body's immunity and metabolism by a large amount of active materials, improve the cultivated animals general level of the health.5 kinds of Chinese herbal medicine mix preparations of the use such as Punitha huge grouper of throwing something and feeding, the test of throwing something and feeding of 60 days shows, the Chinese herbal medicine that basestocks adds 400mg/kg can significantly improve huge grouper survival rate, growth performance and immunity, improves serum activate the phagocytic capacity and Archon ratio.The people such as Choi make mix preparation with the Radix Astragali, the coptis, Herba Andrographitis and kuh-seng four taste Chinese herbal medicines with the ratio of 1:1:2:3, add in feed of grass carp with variable concentrations again, result shows that 2% Chinese herbal medicine interpolation level significantly improves Grass Carp Serum bactericidal activity and total immunoglobulin content, and makes the grass carp of Chinese herbal medicine interpolation group show more high-survival rate [12] under Aeromonas hydrophila is attacked.Chinese herb feed additive is due to characteristics such as its wide material sources, aboundresources, efficient, environmental protection, noresidues, and have the effect of disease-resistant growth-promoting concurrently, application on aquatic products is comparatively extensive, now develop for promoting the fish growths such as hybridized prussian carp [CN 103355482 A], swamp eel [CN 102302098 A], carp [CN 103039756 A], megalobrama amblycephala [CN102524596 A] and improving immune composite Chinese herbal additive, but, less for the research of the additive of the compound Chinese herb of European eel.
Summary of the invention
The object of the present invention is to provide a kind of can be to the compound Chinese herbal medicine additive of European eel disease-resistant growth-promoting.
For achieving the above object, the invention provides a kind of common eel compound Chinese herbal medicine additive of disease-resistant growth-promoting, it is characterized in that, described common eel compound Chinese herbal medicine additive is the mixture of hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, tarragon, the bark of eucommia, the Radix Astragali, Divine Comedy and Fructus Aurantii.
The preparation method of described common eel compound Chinese herbal medicine additive is, get respectively the each raw material equivalent of hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, tarragon, the bark of eucommia, the Radix Astragali, Divine Comedy and Fructus Aurantii, put in freeze-day with constant temperature baking oven 60 DEG C until dry completely, utilize micronizer to pulverize, after pulverizing, cross 120 mesh sieves, and enter together mixing machine and fully mix.
Described common eel compound Chinese herbal medicine additive is for the purposes of eel feed.
Described common eel compound Chinese herbal medicine additive is 0.25-1.25 % by weight for the addition of eel feed.
Described common eel compound Chinese herbal medicine additive is 0.5-1.25 % by weight for the addition of eel feed.
Described common eel compound Chinese herbal medicine additive is 0.5 % by weight for the addition of eel feed
The preparation of compound Chinese herb: get respectively the each raw material equivalent of hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, tarragon, the bark of eucommia, the Radix Astragali, Divine Comedy and Fructus Aurantii, put in freeze-day with constant temperature baking oven 60 DEG C until dry completely, utilize micronizer to pulverize, after pulverizing, cross 120 mesh sieves, and enter together mixing machine and fully mix and obtain compound Chinese herb;
Described compound Chinese herbal medicine additive is by hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, tarragon, the bark of eucommia, the Radix Astragali, these 12 kinds of Chinese herbal medicines of Divine Comedy and Fructus Aurantii, the raw material of getting respectively above-mentioned equivalent is put in freeze-day with constant temperature baking oven 60 DEG C (for more reservation volatile ingredient wherein, the present invention chooses 60 DEG C) until dry completely, utilize micronizer to pulverize, after pulverizing, cross 120 mesh sieves and (can increase Chinese herbal medicine absorptivity, promote the stripping of effective ingredient), and enter together mixing machine and fully mix, gained is compound Chinese herb.Between its plurality of active ingredients, mutually coordinate, produce multi-faceted effect, not only nutritious health-care effect, and directly sterilization, antibacterial, regulate body's immunity and metabolism by a large amount of active materials, improves the cultivated animals general level of the health.
In compound Chinese herbal medicine additive, hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, tarragon, the bark of eucommia are immunologic pattern Chinese herbal medicine; The Radix Astragali, Divine Comedy, Fructus Aurantii are growth-promoting elongated Chinese herbal medicine.Concrete effect is as follows:
Hairyvein agrimony: for the aerial part of rosaceous plant hairyvein agrimony, have astringing to arrest bleeding, only dysentery, the function of removing toxic substances.So its effect with expelling parasite, increase cell resistance power can be used as feed addictive and uses.
Radix Codonopsis: belong to Campanulaceae herbaceos perennial, have tonifying spleen and stomach and easy lung qi, beneficial gas effect to enrich blood, has excitation to nervous system, have and anti-stress, strengthen Abwehrkraft des Koepers, can extend internal antibody life period, improve the effect of animal reproduction performance.
Lucid asparagus: the piece root of liliaceous plant lucid asparagus is conventional Chinese medicine, the effect that have replenishing the vital essence and removing heat, moistening lung nourshing kidney, promotes the production of body fluid to quench thirst.As feed addictive, that lucid asparagus has is antibacterial, strengthen immune effect.
The tuber of dwarf lilyturf: for the piece root of the Liliaceae herbaceos perennial tuber of dwarf lilyturf, there is nourishing Yin and promoting production of body fluid, effect that moistening lung clears away heart-fire.Can improve animal used as test hypoxic tolerance the tuber of dwarf lilyturf, promotes animal used as test antibody to generate and delay antibody and disappear.
Rheum officinale: be being collectively referred to as of perennial plant of multiple polygonaceae Rheum, Chinese herb rhubarb has attacks stagnant, clearing away damp-heat, purging intense heat, cool blood, the effect such as the stasis of blood, removing toxic substances of dispelling.The Rhein that contains in rheum officinale, archen, aloe-emodin can kill bacteria and viruses, and wherein contained tannin is a kind of very strong antioxidant, can delaying senility, strengthen immunity.Rheum officinale usually share with other Chinese medicines in aquatic products, can improve test fish survival rate, reaches diseases prevention and the effect of curing the disease.
Radix Isatidis: be the Typical Representative of anti virus herb, in the applicating history of state-owned more than 2,000 years, function is heat-clearing, removing toxic substances, cool blood, relieve sore throat, can treat inflammation, heat poison, infectious disease etc.Feed adds Radix Isatidis can reach antibacterial, worm-expelling, strengthens immunity, improves the effect of premunition.
Honeysuckle a: Plants that is Caprifoliaceae, flower is called honeysuckle, is often considered medicinal material or food materials and uses, and has clearing heat and detoxicating, effect of wind-heat dissipating, its to cultivated animals can play antibacterial, antivirus action and strengthen aspect cultivated animals immunity function remarkably productive.
Tarragon: be the dry leaf of feverfew Chinese mugwort, tarragon is used for the treatment of the existing long history of illness, as traditional Chinese medicine, that tarragon has is antipyretic, diuresis, dysmenorrhoea, eliminate the phlegm, hemostasis, the multiple efficacies such as antibacterial.Nutritious as feed addictive tarragon, can significantly improve weightening finish and the feed conversion rate of cultivated animals, reduce the incidence of disease.
The bark of eucommia: belong to the perennial arbor in temperate zone, medical value is very high.That the bark of eucommia has is nourishing liver and kidney, strengthen muscles and bones, hypotensive effect, has in vivo higher antioxidation, can improve animal body immunologic function, promotes Animal Bone cell and myofibrillar growth, improves collagen of muscle content.
The Radix Astragali: be the dry root of the legume Radix Astragali, medicinal have effects such as tonifying Qi Kaiyang, strengthening exterior and reducing sweat, inducing diuresis to reduce edema, as feed addictive, the Radix Astragali has effect of accelerating body metabolism, promotion animal growth and development, raising product quality, strengthening cultivated animals immunity.
Divine Comedy: be by peppery official, sweet wormwood, the Chinese medicines such as almond are processed rear and flour or wheat bran mixing, make through fermentation, have strengthening the spleen and stomach, the effect helping digestion in adjusting.Thereby Divine Comedy uses and has the feed of promotion and digest and assimilate and reach growth as one of additive.
Fructus Aurantii: be the dry immature fruit of rutaceae bitter orange and variety thereof.There is relieving stagnant Qi long-pending, the aid digestion effect that disappears.What Fructus Aurantii and the Radix Astragali, Divine Comedy coupling can promote feed digests and assimilates, accelerates body anabolism function, thereby reaches growth promotion, improves the effect of weightening finish.
Above-mentioned 12 kinds of Chinese herbal medicines have effective, and resource is extensively cheap, is well suited for propagating artificially on a large scale the feature of use, and with the selected herbal mixture compatibility difference of prior art, proportioning is also different, and it is easy that equivalent is pulverized and mixed technological operation, easy-to-use.What selected Chinese herbal medicine had promotes emphatically growth, some promotion digestion, and some raising immunity, combine them can improve in all directions each physiological function of cultivation eel body, thereby promote the disease-resistant growth-promoting effect of Chinese herb feed additive.
Embodiments of the invention have verified that described common eel compound Chinese herbal medicine additive can play disease-resistant growth-promoting effect in the cultivation of Europe eel, has the effect that improves European eel growth performance, body composition crude protein content, enteron aisle digestive ferment and sero-immunity enzyme.
Detailed description of the invention
Describe embodiments of the invention below in detail, the example of described embodiment is intended to for explaining the present invention, and can not be interpreted as limitation of the present invention.Unreceipted concrete technology or condition person in embodiment, according to the described technology of the document in this area or condition or carry out according to product description.The unreceipted person of production firm of agents useful for same or instrument, being can be by the conventional products of commercial acquisition.
Traditional Chinese medicine ingredients used in following examples all can obtain in shop of Chinese medicines's purchase.
Embodiment 1: the preparation of common eel compound Chinese herbal medicine additive
The raw material of getting respectively hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, tarragon, the bark of eucommia, the Radix Astragali, Divine Comedy, Fructus Aurantii equivalent is put in freeze-day with constant temperature baking oven 60 DEG C until dry completely, utilize micronizer to pulverize, after pulverizing, cross 120 mesh sieves, and enter together mixing machine and fully mix.
Embodiment 2: compliance test result test
The black young material of common eel that the basestocks that test is used is produced for Fujian heavenly steed feed company, compound Chinese herbal medicine additive is designed to ratio according to arithmetic progression and mixes by enlargement method step by step with basal feed, the feed that is mixed with control group (0%), Diet1 (0.25%), Diet2 (0.50%), Diet3 (0.75%), Diet4 (1.00%), (1.25%) six kind of different interpolation level of Diet5, in valve bag ,-4 DEG C of Refrigerator stores are for subsequent use.Above percentage is weight percentage.
Culture experiment carries out in the glass aquarium (120L) of Collects The American University's seawater test site circulating filtration water system, after two weeks raise and train, pick out the elver physically fit, specification is consistent 540 tails [initial body weight is (6.28 ± 0.03) g], assign at random in 18 aquariums.During formal test every day throw something and feed 2 times (8:00, the 18:00) that be satiated with food, before throwing something and feeding, make bulk feed by group respectively at every turn, after fish ingests 20min, suck residual bait and ight soil and change the aeration running water of 20 volume %.Observe that common eel ingests and growing state every day, record death toll and water temperature.Survey weekly water quality, duration of test water temperature is 22.10 ± 0.21 DEG C, dissolved oxygen concentration >7.6mg/L, ammonia nitrogen concentration <0.25mg/L.Culture-cycle is 42 days.
When the long performance indications of test sashimi (raw fish) are measured, formula used is:
Specific growth rate SGR (%/d)=100 × (ln fish body end weight-ln fish body initial weight/test number of days
Rate of body weight gain WG (%)=100 × (fish body end weight-fish body initial weight)/fish body initial weight;
Survival rate SR (%)=100 × (results mantissa/put in a suitable place to breed mantissa);
Liver body index HSI (%)=100 × (liver pancreas weight/quality of fishes);
Coefficient of condition CF (%)=100 × weight (g)/body is long
3(cm
3)
Fish body composition and feed basis all adopt 105 DEG C of normal pressure oven drying constant weight methods to survey moisture; Adopt Micro-kjoldahl method (total nitrogen × 6.25) to survey crude protein; Soxhlet extraction process (ether is extract) is surveyed crude fat; Muffle furnace calcination method (550 DEG C) is surveyed ash content.In addition, the total superoxide dismutase of enteron aisle amylase, lipase, tryptic activity and serum, lysozyme, alkaline phosphatase activities and the Total albumen content all adopt Nanjing to build up the corresponding reagent box mensuration that Bioengineering Research Institute produces, and operating procedure is carried out to specifications.
The present invention's its main nutrient composition of basal feed used is in table 1:
Table 1 European eel basal feed main nutrient composition table (dry weight %)
Specific experiment result is as table 2-table 5.
The affect table of table 2 compound Chinese herbal medicine additive on European eel growth
Note: colleague's subscript lowercase difference person represents significant difference (P<0.05).
As can be seen from Table 2, basal feed adds this additive can significantly improve European eel rate of body weight gain, specific growth rate and survival rate (P<0.05), compared with control group, interpolation group Europe eel rate of body weight gain has not improved 7.8%-26.1% not etc., reaches maximum (P<0.05) at Diet2 (0.50%) group rate of body weight gain.The interpolation concentration of 0.5%-1.25% makes Europe eel obtain good growth and higher survival rate (P<0.05).Along with adding the rising of concentration, liver body exponential sum coefficient of condition presents and first increases the trend of falling afterwards, but difference less (P>0.05).
Table 3 compound Chinese herbal medicine additive affects table (weight in wet base %) to European eel body composition
Note: same column subscript lowercase difference person represents significant difference (P<0.05), lower same.Fish body weight in wet base refers to the gross weight of moisture content.
As can be seen from Table 3, compound Chinese herbal medicine additive can improve Europe eel body crude protein content within the specific limits, Diet3 (0.75%) group crude protein content reach maximum and with significantly (P<0.05) of contrast difference.Additive has the trend that reduces moisture in body composition, but on crude fat and content of ashes impact less (P>0.05).
The affect table of table 4 compound Chinese herbal medicine additive on European eel Activity of Digestive Enzymes in Intestine
As can be seen from Table 4, European eel its enteron aisle trypsase and amylase under the stimulation of Chinese herbal medicine are all improved, Diet2-Diet5 group Europe eel enteron aisle trypsase is all apparently higher than control group and reach significant difference (P<0.05), Diet2, Diet3 and Diet4 group Europe eel Amylase Activity is in higher level together and Diet4 group activity will reach significant difference (P<0.05) apparently higher than control group.Lipase active is subject to the impact of additive less (P>0.05).
The affect table of table 5 compound Chinese herbal medicine additive on European eel immunologic function
Can find out from GB 5, compound Chinese herbal medicine additive of the present invention can significantly improve the immune indexes (P<0.05) of European eel, the total superoxide dismutase activity of all interpolation groups Europe eel serum is all apparently higher than control group (P<0.05), and diet1, diet2 group is active is significantly higher than other each group in the highest.Serum lysozyme activities reached and total protein content are along with the rising of additive concentration is all the trend of falling after rising, diet2, diet3 group lysozyme activity is in highest level, be significantly higher than diet1 and control group, each additive group total protein concentration of serum all reaches maximum horizontal higher than control group and in diet2 group.Diet1 group Serum alkaline phosphatase activity is significantly higher than control group, but there is no significant difference between other each group.
Comprehensive analysis, under this experiment condition, compound Chinese herbal medicine additive of the present invention can play disease-resistant growth-promoting effect in the cultivation of Europe eel, and this experiment diet2 group is added concentration 0.5% for improving the optimum addition of European eel growth performance, body composition crude protein content, enteron aisle digestive ferment and sero-immunity enzyme.
Although illustrated and described embodiments of the invention above, be understandable that, above-described embodiment is exemplary, can not be interpreted as limitation of the present invention, those of ordinary skill in the art can change above-described embodiment within the scope of the invention in the situation that not departing from principle of the present invention and aim, amendment, replacement and modification.
Claims (6)
1. the common eel compound Chinese herbal medicine additive of a disease-resistant growth-promoting, it is characterized in that, described common eel compound Chinese herbal medicine additive is the mixture of hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, tarragon, the bark of eucommia, the Radix Astragali, Divine Comedy and Fructus Aurantii.
2. common eel compound Chinese herbal medicine additive described in claim 1, it is characterized in that, the preparation method of described common eel compound Chinese herbal medicine additive is, get respectively the each raw material equivalent of hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, tarragon, the bark of eucommia, the Radix Astragali, Divine Comedy and Fructus Aurantii, put in freeze-day with constant temperature baking oven 60 DEG C until dry completely, utilize micronizer to pulverize, after pulverizing, cross 120 mesh sieves, and enter together mixing machine and fully mix.
Described in claim 1 common eel compound Chinese herbal medicine additive for the purposes of eel feed.
4. described in claim 3, common eel compound Chinese herbal medicine additive, for the purposes of eel feed, is characterized in that, described common eel compound Chinese herbal medicine additive is 0.25-1.25 % by weight for the addition of eel feed.
5. described in claim 4, common eel compound Chinese herbal medicine additive, for the purposes of eel feed, is characterized in that, described common eel compound Chinese herbal medicine additive is 0.5-1.25 % by weight for the addition of eel feed.
6. described in claim 5, common eel compound Chinese herbal medicine additive, for the purposes of eel feed, is characterized in that, described common eel compound Chinese herbal medicine additive is 0.5 % by weight for the addition of eel feed.
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| CN105124198A (en) * | 2015-08-07 | 2015-12-09 | 海宁和心饲料有限公司 | Feed for eels and processing process of feed |
| CN105192298A (en) * | 2015-11-11 | 2015-12-30 | 浙江锦天生物科技有限公司 | Composite microecological feed additive for anguilla japonica |
| CN108041345A (en) * | 2017-12-11 | 2018-05-18 | 中山大学 | A kind of America of disease-resistant growth-promoting is into eel fly-maggot protein fodder additive and its preparation method and application |
| CN108041346A (en) * | 2017-12-11 | 2018-05-18 | 中山大学 | A kind of America elver fly-maggot protein fodder additive and its preparation method and application of disease-resistant growth-promoting |
| CN109258983A (en) * | 2018-08-09 | 2019-01-25 | 集美大学 | A kind of Chinese herb feed additive and method promoting the Japanese eel development of ovary |
| CN111743050A (en) * | 2020-08-04 | 2020-10-09 | 珠海海龙生物科技有限公司 | Compound feed for improving survival rate of juvenile sea bass and preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105124198A (en) * | 2015-08-07 | 2015-12-09 | 海宁和心饲料有限公司 | Feed for eels and processing process of feed |
| CN105192298A (en) * | 2015-11-11 | 2015-12-30 | 浙江锦天生物科技有限公司 | Composite microecological feed additive for anguilla japonica |
| CN108041345A (en) * | 2017-12-11 | 2018-05-18 | 中山大学 | A kind of America of disease-resistant growth-promoting is into eel fly-maggot protein fodder additive and its preparation method and application |
| CN108041346A (en) * | 2017-12-11 | 2018-05-18 | 中山大学 | A kind of America elver fly-maggot protein fodder additive and its preparation method and application of disease-resistant growth-promoting |
| CN108041345B (en) * | 2017-12-11 | 2021-04-09 | 中山大学 | Disease-resistant growth-promoting maggot protein feed additive for adult American eels and preparation method and application thereof |
| CN109258983A (en) * | 2018-08-09 | 2019-01-25 | 集美大学 | A kind of Chinese herb feed additive and method promoting the Japanese eel development of ovary |
| CN111743050A (en) * | 2020-08-04 | 2020-10-09 | 珠海海龙生物科技有限公司 | Compound feed for improving survival rate of juvenile sea bass and preparation method and application thereof |
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| CN104187091B (en) | 2017-06-23 |
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Effective date of registration: 20230203 Address after: 363200 Xiongcuo Village, Zhangpu Yanchang, Zhangpu County, Zhangzhou City, Fujian Province Patentee after: Zhangpu Yanchang Jinxing aquaculture Co.,Ltd. Address before: No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, 361000 Patentee before: JIMEI University |