CN104187091B - A kind of common eel compound Chinese herbal medicine additive of disease-resistant growth-promoting - Google Patents
A kind of common eel compound Chinese herbal medicine additive of disease-resistant growth-promoting Download PDFInfo
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- CN104187091B CN104187091B CN201410393424.9A CN201410393424A CN104187091B CN 104187091 B CN104187091 B CN 104187091B CN 201410393424 A CN201410393424 A CN 201410393424A CN 104187091 B CN104187091 B CN 104187091B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention discloses a kind of common eel compound Chinese herbal medicine additive of disease-resistant growth-promoting.It is by hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, folium artemisiae argyi, the bark of eucommia, the Radix Astragali, Divine Comedy and each raw material equivalent of Fructus Aurantii, 60 DEG C are put in freeze-day with constant temperature baking oven until drying completely, crushed using micronizer, after crushing cross 120 mesh sieves, and enter together mixing machine carry out fully mix prepare.It is added in basal feed and feeds common eel, disease-resistant growth-promoting effect can be played in Europe eel cultivates, has functions that to improve European eel growth performance, body composition crude protein content, enteron aisle digestive ferment and sero-immunity enzyme.
Description
Technical field
The present invention relates to field of fodder, more particularly to a kind of disease-resistant growth-promoting common eel compound Chinese herbal medicine additive.
Background technology
European eel delicious meat, nutritious, with clearing heat and detoxicating, stasis eliminatings life is new, the multiple efficacies, warp such as nourishing is taken good care of
Ji value is high, is a kind of domestic and international rare fish salable.With the popularization of intensive culture scale, China supports eel industry and obtains
To fast development, however some regional blindly raising cultivation densities, thinking little of environmental health cultivation, quality safety management exists scarce
Fall into, cause eel disease to break out again and again.Although the positive work that various fishing medicine antibiotic can also be played and improve survival rate, reduce loss
With, but the problems such as bring the resistance to the action of a drug, environmental pollution, medicament residue, it is restricted its application.Nowadays, in the urgent need to seeking
Seeking a kind of new way can fundamentally improve fish autoimmunity, and rapid alleviation, stress reduce disease, while can reach
The purpose of volume increase.
Chinese herb feed additive is the Chinese herbal medicine by various flavor function according to Chinese veterinarian's theory physiological requirements different with animal
It is formulated.Its active ingredient mainly has：Polysaccharide, alkaloid, protein, amino acid, glucoside, grease, wax, resinae, plant
Look for element, mineral matter element and vitamin etc..The effect of these materials is mutually coordinated, produces multi-faceted effect, not only nutritious
Health-care effect, and can be directly sterilized, antibacterial, body's immunity and metabolism are adjusted by a large amount of active materials, carry
The cultivated animals general level of the health high.5 kinds of Chinese herbal medicine mix preparations such as Punitha feed huge grouper, feed experiment table within 60 days
Bright, the Chinese herbal medicine of basestocks addition 400mg/kg can significantly improve huge grouper survival rate, growth performance and immunity, improve blood
Clear phagocytic activity and Archon ratio.Choi et al. Radixs Astragali, the coptis, Herba Andrographitis and the taste Chinese herbal medicine of kuh-seng four are with 1:1:2:3 ratio
Example is made mix preparation, then is added in feed of grass carp with various concentrations, as a result shows that 2% Chinese herbal medicine pitch-based sphere is significantly carried
Grass Carp Serum bactericidal activity high and total immunoglobulin content, and make Chinese herbal medicine addition group in the case where Aeromonas hydrophila is attacked
Grass carp shows more high-survival rate [12].Chinese herb feed additive is due to its wide material sources, aboundresources, efficient, environmental protection, noresidue
Etc. characteristic, and have the effect of disease-resistant growth-promoting concurrently, the application on aquatic products is relatively broad, be developed for promoting different educating silver
Crucian carp [A of CN 103355482], swamp eel [A of CN 102302098], carp [A of CN 103039756], megalobrama amblycephala
Fish growths such as [CN102524596 A] and the immune composite Chinese herbal additive of raising, however, for the compound of European eel
The research of the additive of Chinese herbal medicine is less.
The content of the invention
It is an object of the invention to provide it is a kind of can be to the compound Chinese herbal medicine additive of European eel disease-resistant growth-promoting.
To achieve the above object, the present invention provides a kind of common eel compound Chinese herbal medicine additive of disease-resistant growth-promoting, and its feature exists
In, the common eel compound Chinese herbal medicine additive be hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, folium artemisiae argyi,
The mixture of the bark of eucommia, the Radix Astragali, Divine Comedy and Fructus Aurantii.
The preparation method of the common eel compound Chinese herbal medicine additive is to take hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, big respectively
Huang, Radix Isatidis, honeysuckle, folium artemisiae argyi, the bark of eucommia, the Radix Astragali, Divine Comedy and each raw material equivalent of Fructus Aurantii, put in freeze-day with constant temperature baking oven 60 DEG C until
Dry completely, crushed using micronizer, 120 mesh sieves are crossed after crushing, and enter mixing machine together and fully mixed.
The common eel compound Chinese herbal medicine additive is used for the purposes of eel feed.
The addition that the common eel compound Chinese herbal medicine additive is used for eel feed is 0.25-1.25 weight %.
The addition that the common eel compound Chinese herbal medicine additive is used for eel feed is 0.5-1.25 weight %.
The addition that the common eel compound Chinese herbal medicine additive is used for eel feed is 0.5 weight %
The preparation of compound Chinese herb：Hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, Chinese mugwort are taken respectively
Leaf, the bark of eucommia, the Radix Astragali, Divine Comedy and each raw material equivalent of Fructus Aurantii, put in freeze-day with constant temperature baking oven 60 DEG C until dry completely, using Ultramicro-powder
Broken machine is crushed, and 120 mesh sieves are crossed after crushing, and enters mixing machine together and fully mix to obtain final product compound Chinese herb；
The compound Chinese herbal medicine additive is by hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, Chinese mugwort
Leaf, the bark of eucommia, the Radix Astragali, Divine Comedy and Fructus Aurantii this 12 kinds of Chinese herbal medicines, the raw material that above-mentioned equivalent is taken respectively is put 60 in freeze-day with constant temperature baking oven
DEG C (retaining volatile ingredient therein in order to more, the present invention chooses 60 DEG C) until dry completely, is crushed using micronizer,
120 mesh sieves (can increase Chinese herbal medicine absorptivity, promote the dissolution of effective ingredient) are crossed after crushing, and enters mixing machine together is carried out
Fully mix, gained is compound Chinese herb.It is mutually coordinated between its plurality of active ingredients, multi-faceted effect is produced, do not only have
Nutrition health-care functions, and can be directly sterilized, antibacterial, adjust body's immunity and new old generation by a large amount of active materials
Thank, improve the cultivated animals general level of the health.
Hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, folium artemisiae argyi, Du in compound Chinese herbal medicine additive
Secondary is immunologic pattern Chinese herbal medicine；The Radix Astragali, Divine Comedy, Fructus Aurantii are growth-promoting elongated Chinese herbal medicine.Specific effect is as follows：
Hairyvein agrimony：It is the aerial part of rosaceous plant hairyvein agrimony, there is astringing to arrest bleeding, stop dysentery, the function of removing toxic substances.It has
Expelling parasite, the effect of increase cell resistance power as feed addictive so can be used.
Radix Codonopsis：Category Campanulaceae herbaceos perennial, easy lung qi, the effect of QI invigorating to enrich blood, to god with tonifying spleen and stomach
There is excitation through system, with resisting stress, enhancing Abwehrkraft des Koepers, internal antibody existence time can be extended, improve animal numerous
Grow the effect of performance.
Lucid asparagus：The root tuber of liliaceous plant lucid asparagus, is conventional Chinese medicine, has replenishing the vital essence and removing heat, moistening lung nourshing kidney, promotes the production of body fluid to quench thirst
The effect of.Used as feed addictive, lucid asparagus has the immune effect of antibacterial, enhancing.
The tuber of dwarf lilyturf：It is the root tuber of the Liliaceae herbaceos perennial tuber of dwarf lilyturf, with nourishing Yin and promoting production of body fluid, effect of moistening lung clears away heart-fire.
The tuber of dwarf lilyturf can improve experimental animal hypoxic tolerance, promote experimental animal antibody tormation and delay antibody to disappear.
Rheum officinale：It is that the perennial plant of various polygonaceae Rheums is collectively referred to as, Chinese herb rhubarb has attacks stagnant, clearing away damp-heat, rushes down
The effects such as fire, cool blood, dissolving stasis, removing toxic substances.The Rhein that contains in rheum officinale, rheum emodin, aloe-emodin can kill bacterium and disease
Poison, tannin contained therein is a kind of very strong antioxidant, being capable of delaying senility, strengthen immunity.Rheum officinale is normal in aquatic products
Often shared with other Chinese medicines, it is possible to increase test fish survival rate, reach diseases prevention and the effect of curing the disease.
Radix Isatidis：It is the Typical Representative of anti virus herb, has the applicating history of more than 2,000 years in China, function is heat-clearing,
Removing toxic substances, cool blood, relieving sore-throat can treat inflammation, heat toxin, infectious disease etc..Feed addition Radix Isatidis can reach antibacterial, worm-expelling, strengthen
Immunity, improves the effect of premunition.
Honeysuckle：It is a kind of plant of Caprifoliaceae, flower is referred to as honeysuckle, is often considered medicinal material or food materials are used, with clear
Thermal detoxification, effect of wind-heat dissipating, it can play antibacterial, antivirus action to cultivated animals and strengthen cultivated animals immunization machine
Can aspect effect protrusion.
Folium artemisiae argyi：It is the dried leaf of feverfew Chinese mugwort, folium artemisiae argyi is used to treat the existing long history of illness, used as in tradition
Medicine, folium artemisiae argyi has the multiple efficacies such as antipyretic, diuresis, dysmenorrhoea, eliminating the phlegm, hemostasis, antibacterial.It is rich as feed addictive folium artemisiae argyi nutrition
Richness, can significantly improve weightening and the feed conversion rate of cultivated animals, reduce the incidence of disease.
The bark of eucommia：The category perennial arbor in temperate zone, medical value is very high.The bark of eucommia have it is nourishing liver and kidney, strengthen muscles and bones, hypotensive
Effect, in vivo with antioxidation higher, can improve animal body immunologic function, promote animal osteocyte and muscle fibre
Growth, improve collagen of muscle content.
The Radix Astragali：It is the dry root of the legume Radix Astragali, it is medicinal with the effect such as tonifying Qi Kaiyang, strengthening exterior and reducing sweat, inducing diuresis to remove edema,
Used as feed addictive, the Radix Astragali has to be accelerated body substance metabolism, promotes animal growth and development, raising product quality, enhancing to support
The effect of growing animal immune.
Divine Comedy：It is the sweet wormwood by peppery official, mixes with flour or wheat bran after the Chinese medicine processing such as almond, it is fermented to be made, have
Strengthening the spleen and stomach, the effect helped digestion in adjusting.Divine Comedy is absorbed so as to reach life as one of additive using with promotion feed digestion
Effect long.
Fructus Aurantii：Immature fruit is dried for rutaceae bitter orange and its variety.It is aid digestion with relieving stagnant Qi disperse accumulations
The effect of.Fructus Aurantii can promote digesting and assimilating, accelerating body anabolism function, so as to reach for feed with the Radix Astragali, Divine Comedy combination
Growth promotion, improves the effect of weightening.
Above-mentioned 12 kinds of Chinese herbal medicines have effect good, and resource is cheap extensively, are well suited for propagating what is used artificially on a large scale
Feature, and the herbal mixture compatibility difference with selected by prior art, proportioning are also different, and equivalent is pulverized and mixed technological operation letter
Just, it is easy-to-use.What selected Chinese herbal medicine had promotes emphatically growth, some promotion digestion, some raisings to be immunized, and they are combined energy
Enough each physiological functions of the cultivation eel body of raising in all directions, so as to lift the disease-resistant growth-promoting effect of Chinese herb feed additive.
Embodiments of the invention demonstrate the common eel compound Chinese herbal medicine additive and can play disease-resistant in Europe eel cultivates
Growth promoting effect, with the work for improving European eel growth performance, body composition crude protein content, enteron aisle digestive ferment and sero-immunity enzyme
With.
Specific embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is intended to be used to explain the present invention, and can not
It is interpreted as limitation of the present invention.In the examples where no specific technique or condition is specified, according to described by document in the art
Technology or condition or carried out according to product description.Agents useful for same or the unreceipted production firm person of instrument, being to lead to
Cross city available from conventional products.
Traditional Chinese medicine ingredients used can be commercially available with shop of Chinese medicines in following examples.
Embodiment 1：The preparation of common eel compound Chinese herbal medicine additive
Take respectively hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, folium artemisiae argyi, the bark of eucommia, the Radix Astragali, Divine Comedy,
The raw material of Fructus Aurantii equivalent puts in freeze-day with constant temperature baking oven 60 DEG C until dry completely, is crushed using micronizer, and 120 are crossed after crushing
Mesh sieve, and enter mixing machine together and fully mixed.
Embodiment 2：Compliance test result test
The basestocks that experiment is used are the black young material of the common eel of Fujian heavenly steed feed companies production, and compound Chinese herbal medicine additive is pressed
Design proportional according to arithmetic progression and mixed by enlargement method step by step with basal feed, be configured to control group (0%), Diet1
(0.25%), the different additions of Diet2 (0.50%), Diet3 (0.75%), Diet4 (1.00%), (1.25%) six kind of Diet5
The feed of level, it is standby in -4 DEG C of Refrigerator stores in valve bag.Above percentage is weight percentage.
Culture experiment is carried out in the glass aquarium (120L) of Collects The American University's seawater test site circulating filtration water system, warp
The consistent tail of elver 540 of physically fit, specification [original body mass is (6.28 ± 0.03) g], random point are picked out after raising and train within two weeks
To in 18 aquariums.It is satiated with food daily during formal test and is fed 2 times (8:00,18:00) made by group respectively before, feeding every time
Bulk feed, residual bait and excrement are sucked after fish ingests 20min and the aeration running water of 20 volume % is changed.Common eel is observed daily
Ingest and growing state, record death toll and water temperature.Water temperature is 22.10 ± 0.21 DEG C, dissolved oxygen during surveying water quality, experiment weekly
Concentration>7.6mg/L, ammonia nitrogen concentration<0.25mg/L.Culture-cycle is 42 days.
Formula used is during to test fish growth performance index determining：
Specific growth rate SGR (%/d)=100 × (heavy-ln fish bodies initial weight/experiment number of days in ln fish bodies end
Rate of body weight gain WG (%)=100 × (the last weight-fish body initial weight of fish body)/fish body initial weight；
Survival rate SR (%)=100 × (results mantissa/put mantissa in a suitable place to breed)；
Liver body index HSI (%)=100 × (liver pancreas weight/quality of fishes)；
Coefficient of condition CF (%)=100 × weight (g)/body is long3(cm3)
Nutrition components and feed basis survey moisture using 105 DEG C of normal pressure oven drying constant weight methods；Using semimicro
Kjeldahl's method (total nitrogen × 6.25) surveys crude protein；Soxhlet extraction methods (ether is extract) survey crude fat；Muffle furnace calcination method
(550 DEG C) survey ash contents.In addition, enteron aisle amylase, lipase, tryptic activity and serum total number born, bacteriolyze
Enzyme, alkaline phosphatase activities and the Total albumen content build up the corresponding reagent box of Bioengineering Research Institute's production using Nanjing
Determine, operating procedure is carried out to specifications.
Basal feed its main nutrient composition used by the present invention is shown in Table 1：
European eel basal feed main nutrient composition table (dry weight %) of table 1
Specific experiment result such as table 2- tables 5.
The influence table that the compound Chinese herbal medicine additive of table 2 grows to European eel
Note：Colleague subscript lowercase difference person represents significant difference (P<0.05).
From table 2 it can be seen that basal feed adds this additive can significantly improve European eel rate of body weight gain, particular growth
Rate and survival rate (P<0.05), compared with control group, addition group Europe eel rate of body weight gain improves 7.8%-26.1%,
Diet2 (0.50%) group rates of body weight gain reach maximum (P<0.05).The addition concentration of 0.5%-1.25% then makes Europe eel obtain well
Growth and survival rate (P higher<0.05).With the rising of addition concentration, liver body index and coefficient of condition then show and first increase
The trend for dropping afterwards, but the smaller (P of difference>0.05).
Influence table (weight in wet base %) of the compound Chinese herbal medicine additive of table 3 to European eel body composition
Note：Same column subscript lowercase difference person represents significant difference (P<0.05), similarly hereinafter.Fish body weight in wet base refers to moisture content
Gross weight.
From table 3 it can be seen that compound Chinese herbal medicine additive can within the specific limits improve Europe eel body crude protein content,
Diet3 (0.75%) group crude protein contents reach (P maximum and notable with contrast difference<0.05).Additive has in reduction body composition
The trend of moisture, but smaller (P is influenceed on crude fat and content of ashes>0.05).
Influence table of the compound Chinese herbal medicine additive of table 4 to European eel Activity of Digestive Enzymes in Intestine
From table 4, it can be seen that European eel its enteron aisle trypsase and amylase under the stimulation of Chinese herbal medicine are all carried
Height, Diet2-Diet5 groups Europe eel enteron aisle trypsase is obviously higher than control group and reaches significant difference (P<0.05), Diet2,
Diet3 and Diet4 groups Europe eel Amylase Activity is in higher level and Diet4 groups activity together will be reached apparently higher than control group
To significant difference (P<0.05).Lipase active is influenceed smaller (P by additive>0.05).
Influence table of the compound Chinese herbal medicine additive of table 5 to European eel immunologic function
From GB 5 as can be seen that compound Chinese herbal medicine additive of the invention can significantly improve the immune finger of European eel
Mark (P<0.05), all addition group Europe eel serum total number born activity are obviously higher than control group (P<0.05),
Diet1, diet2 group activity are significantly higher than other each groups in highest.Serum lysozyme activities reached and total protein content are with addition
In trend of falling after rising, diet2, diet3 group lysozyme activity are in highest level, are significantly higher than for the rising of agent concentration
Diet1 and control group, each additive group total protein concentration of serum are above control group and reach maximum horizontal in diet2 groups.diet1
Group Serum alkaline phosphatase activity is significantly higher than between control group, but other each groups and has no significant difference.
Comprehensive analysis, compound Chinese herbal medicine additive of the invention can play anti-in the cultivation of Europe eel under this experiment condition
Sick growth promoting effect, this experiment diet2 groups addition concentration 0.5% for improve European eel growth performance, body composition crude protein content,
The optimum addition of enteron aisle digestive ferment and sero-immunity enzyme.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art is not departing from principle of the invention and objective
In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.
Claims (5)
1. a kind of common eel compound Chinese herbal medicine additive of disease-resistant growth-promoting, it is characterised in that the common eel compound Chinese herbal medicine additive
It is the hairyvein agrimony of equivalent, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, folium artemisiae argyi, the bark of eucommia, the Radix Astragali, Divine Comedy and Fructus Aurantii
Mixture；
Its preparation method be take respectively hairyvein agrimony, Radix Codonopsis, lucid asparagus, the tuber of dwarf lilyturf, rheum officinale, Radix Isatidis, honeysuckle, folium artemisiae argyi, the bark of eucommia,
The Radix Astragali, Divine Comedy and each raw material equivalent of Fructus Aurantii, put in freeze-day with constant temperature baking oven 60 DEG C until dry completely, using micronizer powder
It is broken, 120 mesh sieves are crossed after crushing, and enter mixing machine together and fully mixed；
Feed the common eel compound Chinese herbal medicine additive of disease-resistant growth-promoting described in addition 0.25-1.25 weight % in the basal feed of common eel
Compared with the control of common eel compound Chinese herbal medicine additive for being not added with the disease-resistant growth-promoting, the rate of body weight gain of common eel, specific is improve
Growth rate and survival rate；Crude protein content in common eel body, enteron aisle trypsase and Amylase Activity；The total superoxides of serum
Dismutase activity and serum lysozyme activities reached.
2. common eel compound Chinese herbal medicine additive described in claim 1 is used to prepare the purposes of eel feed.
3. common eel compound Chinese herbal medicine additive described in claim 2 is used to prepare the purposes of eel feed, it is characterised in that described
The addition that common eel compound Chinese herbal medicine additive is used for eel feed is 0.25-1.25 weight %.
4. common eel compound Chinese herbal medicine additive described in claim 3 is used to prepare the purposes of eel feed, it is characterised in that described
The addition that common eel compound Chinese herbal medicine additive is used for eel feed is 0.5-1.25 weight %.
5. common eel compound Chinese herbal medicine additive described in claim 4 is used to prepare the purposes of eel feed, it is characterised in that described
The addition that common eel compound Chinese herbal medicine additive is used for eel feed is 0.5 weight %.
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| CN105124198A (en) * | 2015-08-07 | 2015-12-09 | 海宁和心饲料有限公司 | Feed for eels and processing process of feed |
| CN105192298A (en) * | 2015-11-11 | 2015-12-30 | 浙江锦天生物科技有限公司 | Composite microecological feed additive for anguilla japonica |
| CN108041346B (en) * | 2017-12-11 | 2021-05-07 | 中山大学 | Disease-resistant growth-promoting maggot protein feed additive for American young eels and preparation method and application thereof |
| CN108041345B (en) * | 2017-12-11 | 2021-04-09 | 中山大学 | Disease-resistant growth-promoting maggot protein feed additive for adult American eels and preparation method and application thereof |
| CN109258983A (en) * | 2018-08-09 | 2019-01-25 | 集美大学 | A kind of Chinese herb feed additive and method promoting the Japanese eel development of ovary |
| CN111743050A (en) * | 2020-08-04 | 2020-10-09 | 珠海海龙生物科技有限公司 | Compound feed for improving survival rate of juvenile sea bass and preparation method and application thereof |
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| JPS5918026B2 (en) * | 1976-05-19 | 1984-04-25 | 日清製粉株式会社 | Eel feed |
| CN101720869A (en) * | 2009-12-07 | 2010-06-09 | 中国水产科学研究院淡水渔业研究中心 | Method for preparing immunostimulant for fish |
| CN102754734B (en) * | 2012-05-28 | 2013-06-19 | 鲁东大学 | Chinese herbal livestock feed additive and application thereof |
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Effective date of registration: 20230203 Address after: 363200 Xiongcuo Village, Zhangpu Yanchang, Zhangpu County, Zhangzhou City, Fujian Province Patentee after: Zhangpu Yanchang Jinxing aquaculture Co.,Ltd. Address before: No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, 361000 Patentee before: JIMEI University |