CN108041345B - Disease-resistant growth-promoting maggot protein feed additive for adult American eels and preparation method and application thereof - Google Patents
Disease-resistant growth-promoting maggot protein feed additive for adult American eels and preparation method and application thereof Download PDFInfo
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- CN108041345B CN108041345B CN201711308166.XA CN201711308166A CN108041345B CN 108041345 B CN108041345 B CN 108041345B CN 201711308166 A CN201711308166 A CN 201711308166A CN 108041345 B CN108041345 B CN 108041345B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention discloses a disease-resistant growth-promoting maggot protein feed additive for American adult eels and a preparation method and application thereof. The feed additive comprises the following raw materials in parts by mass: 40 to 50 percent of fly maggot protein slurry, 20 to 25 percent of pregelatinized starch, 20 to 30 percent of molasses, 0.1 to 0.5 percent of chitosan oligosaccharide, 1 to 3 percent of soybean lecithin, 1 to 2 percent of vitamin mixture and 1.5 to 2.5 percent of inorganic salt mixture. Aiming at the characteristics of American adult eels, the invention takes maggot protein as a main material, is matched with proper amount of chitosan oligosaccharide and soybean lecithin, and is matched with pregelatinized starch, molasses, vitamin mixture and inorganic salt mixture in a certain proportion to prepare the additive, and various components are coordinated with each other, so that the nutrition condition of the American adult eels can be obviously improved, the growth of the American adult eels is promoted, the feed coefficient is reduced, the disease resistance of the American adult eels is improved, the use amount of medicines (antibiotics) is reduced, and the culture economic benefit is increased.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a disease-resistant and growth-promoting maggot protein feed additive for adult eels, and a preparation method and application thereof.
Background
The American eels (Anguilla prostrata) are one of three varieties of eels cultured in China, have tender and delicious meat quality and rich nutrition, have the effects of nourishing and strengthening the body, are regarded as 'ginseng in water' by people, and are one of important agricultural products for earning foreign exchange in export of China. However, with the increasing of the culture intensification degree, the occurrence of eel diseases is more frequent, the disease death rate is increased year by year, and the direct economic loss is up to 10 hundred million yuan each year. In order to control diseases, culturists have to adopt chemical drugs such as antibiotics for prevention and treatment, and although the method plays a positive role in improving cultivation survival rate, reducing disease loss and the like, the method also brings problems of drug residue, environmental pollution, bacterial drug resistance and the like, so that the application of the method is limited. Therefore, the active search for safe, reliable, pollution-free and residue-free disease prevention and control technology and method has become a problem to be solved urgently in the eel breeding industry in China at present. The research and development of green disease-resistant growth-promoting additives for regulating and controlling fish nutrition and immune function have become the subject of current attention of scholars at home and abroad.
Because the American eels have larger difference in nutrition requirements at different growth and development stages, in the American eel breeding, different raw materials of the artificial compound feed are scientifically mixed to form different artificial compound feeds according to different breeding stages. The American eels have important points of stress resistance and immunity to the outside in addition to the indexes of growth and survival rate in terms of nutritional requirements.
In the prior art, immune performance of the American eels is promoted by adding immune polysaccharide or Chinese herbal medicine preparation into feed, but the problems of high cost, low content of polysaccharide in natural products, difficult separation and extraction, incomplete production technology, difficult quality standard control, complex components, difficult determination of molecular structure and the like exist. Therefore, it is urgent to find a new feed additive which can satisfy the nutritional requirement and enhance the immune function by reducing the excessive dependence on immune polysaccharide.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, provides a disease-resistant growth-promoting maggot protein feed additive for American adult eels, and a preparation method and application thereof, so as to promote the adult eel to grow rapidly, improve the immunity of the adult eels, reduce the use of drugs such as antibiotics and the like, promote the development of healthy culture of the eels and improve the international competitiveness of eel products.
The first purpose of the invention is to provide a maggot protein feed additive for American adult eels, which can resist diseases and promote growth.
The second purpose of the invention is to provide a preparation method of the maggot protein feed additive for the American adult eels.
The third purpose of the invention is to provide the application of the maggot protein feed additive for the American adult eels.
The fourth purpose of the invention is to provide American adult eel feed containing the fly maggot protein feed additive for American adult eels.
The above purpose of the invention is realized by the following technical scheme:
a disease-resistant growth-promoting maggot protein feed additive for American adult eels comprises the following raw materials in parts by mass: 40 to 50 percent of fly maggot protein slurry, 20 to 25 percent of pregelatinized starch, 20 to 30 percent of molasses, 0.1 to 0.5 percent of chitosan oligosaccharide, 1 to 3 percent of soybean lecithin, 1 to 2 percent of vitamin mixture and 1.5 to 2.5 percent of inorganic salt mixture.
According to the invention, through research, the addition of a proper amount of fly maggot protein slurry in the American adult eel feed additive can obviously improve the immunity of adult eels, ensure the nutritional requirements of the adult eels, improve the growth and survival rate of the adult eels and shorten the growth period. The fly maggot protein is a high-quality animal protein, its essential amino acid content is greater than 40% of total amino acid, and said invention uses fly maggot protein as main material, and adds a proper quantity of chitosan oligosaccharide and soybean lecithin, and adds a certain proportion of pregelatinized starch, molasses, vitamin mixture and inorganic salt mixture, and their several components are mutually coordinated so as to produce multi-orientation action, so that it not only has the nutrient health-care action, but also can raise the activity of non-specific immune enzyme related to adult eel, and can raise disease-resisting capacity of adult eel and raise the health level of bred adult eel.
The 7 raw materials have the characteristics of good effect, wide resources, low price and suitability for large-scale artificial breeding, are different in compatibility and proportion from the selected feed or feed additives in the prior art, and are simple and convenient to operate and easy to use in a crushing and mixing process. The selected raw materials are mainly used for promoting growth and are also used for improving immunity, and the combination of the raw materials can comprehensively improve various physiological functions of the cultivated eel organism, thereby improving the disease resistance and growth promotion effects of the feed additive.
In order to realize the purpose of the invention, a proper amount of synergist can be added into the raw materials of the fly maggot protein feed additive for the American adult eels according to requirements, and the sum of the mass percentages of the contained raw materials is ensured to be 100%. The synergist is beneficial microorganism, Chinese herbal medicine, tubificidae powder or fermented soybean meal and the like.
In a preferred embodiment, the fly maggot protein feed additive for the American adult eels is composed of the following raw materials in parts by mass: 45% of fly maggot protein pulp, 23% of pregelatinized starch, 25% of molasses, 0.5% of chitosan oligosaccharide, 3% of soybean lecithin, 1% of vitamin mixture and 2.5% of inorganic salt mixture.
In another preferred embodiment, the fly maggot protein feed additive for the American adult eels is composed of the following raw materials in parts by mass: 50% of fly maggot protein slurry, 23% of pregelatinized starch, 20% of molasses, 0.5% of chitosan oligosaccharide, 3% of soybean lecithin, 1% of vitamin mixture and 2.5% of inorganic salt mixture.
In the invention, the preparation method of the fly maggot protein slurry comprises the following steps: and (3) breeding and separating the wheat bran to obtain maggots, disinfecting with 0.1% potassium permanganate, cleaning, draining, and grinding into slurry.
In the present invention, the molasses includes, but is not limited to, cane molasses, beet molasses, grape molasses, corn molasses, invert molasses and molasses, preferably cane molasses.
The vitamin mixture is a vitamin mixture for eel which is conventionally used in the field of aquatic feeds; preferably, each kilogram of the feed additive comprises the following components in parts by weight: 150 ten thousand IU of vitamin A, 30 ten thousand IU of vitamin D310g of vitamin E, 1g of vitamin K32g of vitamin B14g of vitamin B23g of vitamin B620mg of vitamin B1230g of vitamin C, 50mg of biotin, 0.5g of folic acid, 15g of nicotinic acid, 6g of calcium pantothenate and 20g of inositol.
The inorganic salt mixture is an inorganic salt mixture for eel which is conventionally used in the field of aquatic feeds; preferably, the inorganic salt mixture in each kilogram of feed additive comprises the following components in percentage by weight: 8g of zinc sulfate, 60g of ferrous sulfate, 200g of magnesium sulfate, 2.5g of manganese sulfate, 120g of potassium chloride, 3.5g of copper sulfate, 0.06g of potassium iodate, 0.4g of cobalt chloride, 0.03g of sodium selenite and 50g of sodium dihydrogen phosphate.
The invention also provides a preparation method of the maggot protein feed additive for the American adult eels, which mainly comprises the following steps:
s1, weighing fly maggot protein slurry, pregelatinized starch and molasses according to mass fraction, mixing and uniformly stirring to obtain a premix;
s2, weighing chitosan oligosaccharide, soybean lecithin, a vitamin mixture and an inorganic salt mixture according to the mass fraction, respectively adding the chitosan oligosaccharide, the soybean lecithin, the vitamin mixture and the inorganic salt mixture into the premix obtained in the step S1, and uniformly stirring to obtain a material;
and S3, drying and crushing the material obtained in the step S2, and uniformly mixing to obtain the American adult eel fly maggot protein feed additive.
In the invention, the preparation method of the fly maggot protein slurry comprises the following steps: and (3) breeding and separating the wheat bran to obtain maggots, disinfecting with 0.1% potassium permanganate, cleaning, draining, and grinding into slurry.
Preferably, in the step S3, the drying condition is that the drying is performed at 55 to 60 ℃ until the moisture content of the material is less than 10%.
More preferably, in the step S3, the drying condition is drying at 55 ℃ until the moisture content of the material is less than 10%.
Preferably, in the step S3, the crushing grain size is 120 meshes.
The invention also claims application of the maggot protein feed additive for the American adult eels in preparation of American adult eel feeds.
Preferably, the application is applied to the aspect of improving the immunity of American adult eels.
Particularly preferably, when the disease-resistant growth-promoting maggot protein feed additive for the American adult eels is added into the basic feed for feeding the American adult eels, the weight gain rate, the specific growth rate, the feed coefficient and the survival rate of the American adult eels are obviously improved compared with the control without the disease-resistant growth-promoting maggot protein feed additive; meanwhile, the activity of superoxide dismutase, alkaline phosphatase, acid phosphatase and lysozyme of the American adult eel is obviously improved.
The invention also provides American adult eel feed containing the fly maggot protein feed additive for American adult eels.
Preferably, the addition amount of the maggot protein feed additive for the American adult eels in the feed is 3-5%.
More preferably, the addition amount of the maggot protein feed additive for the American adult eels in the feed is 4%.
Compared with the prior art, the invention has the following beneficial effects:
1. the feed additive prepared by taking the fly maggot protein as the main material has rich nutrition, can effectively improve the nutritional status of the American adult eel organism, promotes the growth of the American adult eel, and simultaneously achieves the purpose of reducing the bait coefficient.
2. The invention takes maggot protein as the main material and is matched with chitosan oligosaccharide and soybean lecithin, thus having the functions of improving immunity, activating cells, regulating the environment of the organism and the like, enhancing the disease resistance of American adult eels and reducing the use of drugs and antibiotics.
3. The feed additive is safe, green, healthy and pollution-free, does not contain any hormone or antibiotic, has simple and feasible production process and is convenient for large-scale popularization; the feed additive can relieve the situation of protein tension of animal feed in China, can enhance the immunity of eel, promotes the production of safe food, reduces the risk of eel culture, improves the income of fishermen, and better promotes the development of eel industry in China.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1A fly maggot protein feed additive for American adult eel for disease resistance and growth promotion
1. Formulation composition
A maggot protein feed additive for American adult eels for resisting diseases and promoting growth takes maggot protein as a main raw material and comprises the following components in percentage by mass: 40% of fly maggot protein slurry, 23% of pregelatinized starch, 30% of molasses, 0.5% of chitosan oligosaccharide, 3% of soybean lecithin, 1% of vitamin mixture and 2.5% of inorganic salt mixture;
wherein, the vitamin mixture in each kilogram of feed additive comprises the following components in percentage by weight: 150 ten thousand IU of vitamin A, 30 ten thousand IU of vitamin D310g of vitamin E, 1g of vitamin K32g of vitamin B14g of vitamin B23g of vitamin B620mg of vitamin B1230g of vitamin C, 50mg of biotin, 0.5g of folic acid, 15g of nicotinic acid, 6g of calcium pantothenate and 20g of inositol;
wherein, the inorganic salt mixture in each kilogram of feed additive comprises the following components in percentage by weight: 8g of zinc sulfate, 60g of ferrous sulfate, 200g of magnesium sulfate, 2.5g of manganese sulfate, 120g of potassium chloride, 3.5g of copper sulfate, 0.06g of potassium iodate, 0.4g of cobalt chloride, 0.03g of sodium selenite and 50g of sodium dihydrogen phosphate.
2. Preparation method
The preparation method of the disease-resistant growth-promoting maggot protein feed additive for the adult eels comprises the following steps:
(1) sterilizing maggots obtained by culturing and separating wheat bran by using 0.1% potassium permanganate, cleaning, filtering, and grinding to obtain maggot protein pulp; weighing the prepared fly maggot protein slurry, the pre-gelatinized starch and the molasses according to the mass parts, mixing and uniformly stirring to obtain a premix;
(2) weighing chitosan oligosaccharide, soybean lecithin, a vitamin mixture and an inorganic salt mixture according to the mass parts, respectively adding the chitosan oligosaccharide, the soybean lecithin, the vitamin mixture and the inorganic salt mixture into the premix obtained in the step (1), and mixing in a stirrer to obtain a uniformly mixed material;
(3) drying the material prepared in the step (2) at the temperature of 55-60 ℃ until the moisture content is lower than 10% to obtain dry powder; micronizing the dry powder, sieving with 120 mesh sieve, and mixing to obtain maggot protein feed additive for American adult eel.
3. Application method
4.5% of the maggot protein feed additive for the adult eels in the embodiment is added into the adult eels basic feed.
Example 2A maggot protein feed additive for American adult eel for disease resistance and growth promotion
1. Formulation composition
A maggot protein feed additive for American adult eels for resisting diseases and promoting growth takes maggot protein as a main raw material and comprises the following components in percentage by mass: 45% of fly maggot protein pulp, 23% of pregelatinized starch, 25% of molasses, 0.5% of chitosan oligosaccharide, 3% of soybean lecithin, 1% of vitamin mixture and 2.5% of inorganic salt mixture.
2. Preparation method
The preparation method is the same as that of example 1.
3. Application method
4.5% of the maggot protein feed additive for the adult eels in the embodiment is added into the adult eels basic feed.
Example 3A fly maggot protein feed additive for American adult eel for disease resistance and growth promotion
1. Formulation composition
A maggot protein feed additive for American adult eels for resisting diseases and promoting growth takes maggot protein as a main raw material and comprises the following components in percentage by mass: 50% of fly maggot protein slurry, 23% of pregelatinized starch, 20% of molasses, 0.5% of chitosan oligosaccharide, 3% of soybean lecithin, 1% of vitamin mixture and 2.5% of inorganic salt mixture.
2. Preparation method
The preparation method is the same as that of example 1.
3. Application method
4.5% of the maggot protein feed additive for the adult eels in the embodiment is added into the adult eels basic feed.
Example 4A fly maggot protein feed additive for American adult eel for disease resistance and growth promotion
1. The formulation was the same as in example 2 and the preparation was the same as in example 1.
2. Application method
3% of the maggot protein feed additive for the adult eels in the embodiment is added into the adult eels basic feed.
Example 5A fly maggot protein feed additive for American adult eel for disease resistance and growth promotion
1. The formulation was the same as in example 2 and the preparation was the same as in example 1.
2. Application method
4% of the maggot protein feed additive for the adult eels in the embodiment is added into the adult eels basic feed.
Example 6A fly maggot protein feed additive for American adult eel for disease resistance and growth promotion
1. The formulation was the same as in example 2 and the preparation was the same as in example 1.
2. Application method
The maggot protein feed additive for the adult eels in the example was added to 5% of the adult eels basal feed.
Example 7 Effect verification test
1. Test method
(1) Feeding management
1) Grouping: the feed additives of examples 1 to 6 were added to adult eel basal feeds according to the respective methods of use to conduct feeding experiments, to examine the effect of the american adult eel fly maggot protein feed additives of the present invention on the growth and immunity of american eel adult eels, and the basal feed group to which the american adult eel fly maggot protein feed additives were not added was used as a control.
2) Feeding: the culture test is carried out by hanging a net in a culture pond of a circulating filtration water system in a test field of the Keyang bioengineering Co., Ltd, Wuhan City, with the size of the net cage being 2m multiplied by 1 m. 50 tails of American adult eels (the initial weight is 72.38 +/-0.73 g) with strong physique and consistent specification are put into each net cage, and each experimental group is repeated for 3 times. During the test period, the feed is fed for 2 times (8:00, 18:00) per day after being fed for each time, and the feed is respectively prepared into bulk feed according to groups before each feeding, wherein the feed feeding amount is 3 percent of the total weight of the American eels. The water is changed by 20 percent 1 hour after the bait is thrown every day, and the water temperature is 25-30 ℃ during the test period. The feeding and growth conditions of the eels are observed every day, and the daily bait feeding amount, death number and the like are recorded. The test period is 56 days.
(2) Measurement index
1) Growth index
At the beginning and end of the test, the weight of each group of eels is respectively weighed, the number of eel strips is measured, and the weight gain rate, the specific growth rate, the feed coefficient and the survival rate of relative body mass are calculated. Respectively according to the following formula:
relative weight gain (%) [ (test end mass-test initial mass)/test initial mass ] × 100
Specific growth rate (%/day) [ (ln test end average weight-ln test initial average weight)/test day ] × 100
Feed coefficient ═ feed intake mass/(test end mass-test initial mass)
Survival (%) (number of survivors at end of test/initial total number of test) × 100
2) Non-specific immune index
Randomly taking 10 eels from each test group, collecting blood with tail vein of 1mL syringe, placing the collected blood in 2mL sterilized centrifuge tube, standing at 4 deg.C for 8 hr, 3000rpm for 10min, collecting supernatant, and storing at-80 deg.C for use. The activity of superoxide dismutase, acid phosphatase, alkaline phosphatase and lysozyme in serum is measured by using a kit produced by Nanjing institute of bioengineering, and the measurement method is carried out according to the kit specification.
(3) Data processing
The experimental data are expressed as mean ± standard deviation (mean ± SD), and the software SPSS19.0 is used to perform One-way ANOVA (One-way-ANOVA), and Duncan's multiple comparisons to analyze the significance of the differences (P <0.05) in the experimental results.
2. Results and analysis
TABLE 1 Effect of fly maggot protein content in additives on American adult eel growth performance
| Group of | Relative weight gain (%) | Specific growth rate (%/day) | Coefficient of feed | Survival rate (%) |
| Control group | 40.23±3.10c | 0.60±0.03c | 1.93±0.24a | 92.38±2.11b |
| Example 1 | 56.67±1.34b | 0.80±0.01b | 1.76±0.12b | 100a |
| Example 2 | 73.67±3.59a | 0.99±0.03a | 1.57±0.25c | 100a |
| Example 3 | 57.92±5.25b | 0.82±0.04b | 1.80±0.30ab | 100a |
As can be seen from Table 1, the relative increase rate, specific growth rate and survival rate of the American adult eels are significantly higher than those of the control group, and the feed coefficient is significantly reduced compared with that of the control group after the feed added with the additive of the invention is fed. The growth promoting effect of the embodiment 2 is the best, the bait coefficient is the lowest, the survival rate is the highest, and the difference is obvious compared with the embodiment 1 and the embodiment 3 (P < 0.05).
TABLE 2 influence of fly maggot protein content in the additives on the non-specific index of American adult eels (U/mL)
As shown in Table 2, the activities of superoxide dismutase, alkaline phosphatase, acid phosphatase and lysozyme in the serum were significantly improved (P <0.05) when the feed supplemented with the additive of the present invention was fed, compared to the control group, and the effect was the best in example 2.
TABLE 3 Effect of the addition of the amount on the growth of American adult eel
As can be seen from table 3, the american adult eels were fed with 3%, 4% and 5% of the feed additive of the formula of example 2 added to the basic feed for american adult eels, respectively. As a result, the relative increase rate, specific growth rate and survival rate of the American adult eels cultured by the three additives are obviously higher than those of the control group, and the feed coefficient is obviously lower than that of the control group, wherein the effect is best realized by the 5 th embodiment.
TABLE 4 influence of the amount added on the nonspecific immunity index of American adult eel (U/mL)
As can be seen from table 4, the american adult eels were fed with 3%, 4% and 5% of the feed additive of the formula of example 2 added to the basic feed for american adult eels, respectively. As a result, it was found that the activities of superoxide dismutase, alkaline phosphatase, acid phosphatase and lysozyme in serum were the highest in example 5, but the indexes were not significantly different from those in example 6; meanwhile, compared with a control group, the activity of 4 enzymes of each group fed with the fly maggot protein feed additive is obviously higher than that of the control group.
Therefore, the feed additive has obvious effects of promoting the growth and development of the American adult eels and improving the immunity of the American adult eels, and has wide market prospect. All modifications which can be derived or suggested directly from the disclosure herein by a person skilled in the art are to be considered within the scope of the present invention.
Claims (9)
1. A disease-resistant growth-promoting maggot protein feed additive for American adult eels is characterized by comprising the following raw materials in parts by mass: 45% of fly maggot protein pulp, 23% of pregelatinized starch, 25% of molasses, 0.5% of chitosan oligosaccharide, 3% of soybean lecithin, 1% of vitamin mixture and 2.5% of inorganic salt mixture.
2. The maggot protein feed additive for the adult eels in america according to claim 1, wherein each kilogram of the feed additive comprises the following components in parts by weight: 150 ten thousand IU of vitamin A, 30 ten thousand IU of vitamin D310g of vitamin E, 1g of vitamin K32g of vitamin B14g of vitamin B23g of vitamin B620mg of vitamin B1230g of vitamin C and 50mg of biotin0.5g folic acid, 15g nicotinic acid, 6g calcium pantothenate, 20g inositol.
3. The maggot protein feed additive for the adult eels in america according to claim 1, wherein the inorganic salt mixture in each kilogram of feed additive comprises the following components in parts by weight: 8g of zinc sulfate, 60g of ferrous sulfate, 200g of magnesium sulfate, 2.5g of manganese sulfate, 120g of potassium chloride, 3.5g of copper sulfate, 0.06g of potassium iodate, 0.4g of cobalt chloride, 0.03g of sodium selenite and 50g of sodium dihydrogen phosphate.
4. The maggot protein feed additive for adult eels in america according to claim 1, wherein the preparation method comprises the steps of:
s1, weighing fly maggot protein slurry, pregelatinized starch and molasses according to mass fraction, mixing and uniformly stirring to obtain a premix;
s2, weighing chitosan oligosaccharide, soybean lecithin, a vitamin mixture and an inorganic salt mixture according to the mass fraction, respectively adding the chitosan oligosaccharide, the soybean lecithin, the vitamin mixture and the inorganic salt mixture into the premix obtained in the step S1, and uniformly stirring to obtain a material;
and S3, drying and crushing the material obtained in the step S2, and uniformly mixing to obtain the American adult eel fly maggot protein feed additive.
5. The maggot protein feed additive for adult eels in america according to claim 4, wherein in the step S3, the drying condition is drying at 55-60 ℃ until the moisture of the material is less than 10%.
6. Use of the maggot protein feed additive for adult eels according to any one of claims 1 to 5 in the preparation of adult eels feed.
7. A feed for adult American eels, comprising the maggot protein feed additive for adult American eels according to claim 1.
8. The American adult eel feed as claimed in claim 7, wherein the amount of the American adult eel maggot protein feed additive added in the feed is 3-5%.
9. The eel feed as claimed in claim 8, wherein the fly maggot protein feed additive is added into the feed in an amount of 4%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711308166.XA CN108041345B (en) | 2017-12-11 | 2017-12-11 | Disease-resistant growth-promoting maggot protein feed additive for adult American eels and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711308166.XA CN108041345B (en) | 2017-12-11 | 2017-12-11 | Disease-resistant growth-promoting maggot protein feed additive for adult American eels and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108041345A CN108041345A (en) | 2018-05-18 |
| CN108041345B true CN108041345B (en) | 2021-04-09 |
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| CN1269153A (en) * | 1999-04-05 | 2000-10-11 | 唐耿吉 | Production process of maggot protein additivie for fish feed |
| CN101978858A (en) * | 2010-11-19 | 2011-02-23 | 福建天马饲料有限公司 | Powder compound feed for adult Japanese eels |
| CN102771678A (en) * | 2012-08-16 | 2012-11-14 | 福建天马饲料有限公司 | Powdery compound feed for anguilla marmorata adult stage |
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| CN107307173A (en) * | 2017-07-10 | 2017-11-03 | 罗乌支 | A kind of method that active protein powder is prepared with housefly maggot |
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