CN103583928A - Feed used for improving immune functions of grass carp - Google Patents

Abstract

The invention provides a feed used for improving immune functions of grass carp. The feed comprises rapeseed oil, soybean meal, fish meal, salt, wheat flour, a traditional Chinese medicine immune preparation, bacillus subtilis, a mineral element additive, a vitamin additive and calcium dihydrogen phosphate. According to the invention, the feed including the traditional Chinese medicine immune preparation has the advantages of high efficiency, small toxic and side effects, high security, little residual, etc., has widely available raw materials and low cost and can adjust the immune system of fish and improve anti-stress capability and immune functions of aquatic animals, thereby preventing and treating fish diseases.

Description

For improving the feed of grass carp immunity function

Technical field

The present invention relates to traditional Chinese medicine technical field, relate in particular to a kind of for improving the feed of grass carp immunity function.

 

Background technology

Along with the development of aquatic products intensive degree, multiple, complicated, the high feature endangering that aquatic animal disease progressively presents, the deterioration of cultivation water environment also causes fish immunity power to decline.For reply fish disease spreading trend, a large amount of splashing of fish insect killing medicine are used, the abuse of Multiple Classes of Antibiotics and external sterilization medicine are splashed etc., although disease is had to certain control action, but often cause drug resistance, the medicament residue of pathogenic microorganisms, even cause whole water ecological setting balance to destroy.

In recent years, grass carp ( ctenopha ryngodon idellus) because its efficiency of feed utilization is high, aquaculture cost is cheap, it is rapid, best in quality to grow, liked by broad masses, developed into the govern-house-variety of China's freshwater aquiculture.Grass carp is one of China " four large Chinese carps ", but the incidence of disease (the average 50 % left and right due to grass carp " three diseases ", sometimes up to more than 90 %), (generally more than 90 %) are very high for case fatality rate, popular very wide, fish production is made a big impact.In order to solve the problem of preventing and treating of grass carp " three diseases ", need conscientiously to improve the immunity function of grass carp.

Chinese herbal medicine is a kind of desirable natural, environment-friendly medicine; there is little, safe, the residual plurality of advantages such as few of efficient, toxic and side effect; and wide material sources; cost is low, and the immune system of fish is adjusted, and improves moving anti-stress ability and the immunity function of aquatic products; preventing and curing of fish disease; Chinese herbal medicine is as disease prevention and cure medicine or additive of bait, is particularly useful for the needs of intensive, the large-scale production of current culture fishery, is convenient to carry out colony's control of cultured fishes disease.And application herbal control fish disease meets the disease control criterion of the nuisanceless aquatic products industry of development, production green aquatic product completely.The immunity that improves aquatic livestock of using by Chinese herbal medicine, improves animal disease resistant power, and the incidence of disease that reduces aquatic livestock is the focus of studying in recent years.Advocating healthy aquaculture under the new situation at present comprehensively, promoting herbal control fish disease, be extremely important.

The present invention is exactly on this basis, propose a kind of for improving the Chinese medicine immune formulation of grass carp immunity function, it has efficiently, little, safe, the residual plurality of advantages such as few of toxic and side effect, and wide material sources, cost is low, immune system to fish is adjusted, and improves moving anti-stress ability and the immunity function of aquatic products, thus preventing and curing of fish disease.

 

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of for improving the Chinese medicine immune formulation of grass carp immunity function, it has efficiently, little, safe, the residual plurality of advantages such as few of toxic and side effect, and wide material sources, cost is low, immune system to fish is adjusted, improve moving anti-stress ability and the immunity function of aquatic products, thus preventing and curing of fish disease.

For solving the problems of the technologies described above, the invention provides a kind ofly for improving the Chinese medicine immune formulation of grass carp immunity function, its bulk drug comprises umbellate pore furgus, wilsonii, the bighead atractylodes rhizome, folium panacis japonici cum caule, cordate houttuynia, the bark of eucommia, eclipta, the rhizome of davallia, cassia twig, Radix Isatidis, hawthorn, the membrane of a chicken's gizzard, Chinese trumpet creeper, Chinese yam, reticulate millettia, spina gleditsiae, Fructus Corni, peach branch, frutus cnidii, date, the tuber of stemona, Gorgon fruit, Galanga Galangal Seed, betel nut, pittosporum illicioides and Horseweed Herb.

Wherein, in described Chinese medicine immune formulation, the weight of each bulk drug is respectively umbellate pore furgus 25g～35g, wilsonii 45g～55g, bighead atractylodes rhizome 35g～45g, folium panacis japonici cum caule 60g～70g, cordate houttuynia 25g～35g, bark of eucommia 30g～40g, eclipta 35g～45g, rhizome of davallia 30g～40g, cassia twig 25g～35g, Radix Isatidis 35g～45g, hawthorn 35g～45g, the membrane of a chicken's gizzard 25g～35g, Chinese trumpet creeper 20g～30g, Chinese yam 25g～35g, reticulate millettia 35g～45g, spina gleditsiae 25g～35g, Fructus Corni 30g～40g, peach branch 25g～35g, frutus cnidii 35g～45g, date 30g～40g, tuber of stemona 20g～30g, Gorgon fruit 25g～35g, Galanga Galangal Seed 20g～30g, betel nut 25g～35g, pittosporum illicioides 35g～45g and Horseweed Herb 30g～40g.

Wherein, in described Chinese medicine immune formulation, the weight of each bulk drug is respectively umbellate pore furgus 30g, wilsonii 50g, bighead atractylodes rhizome 40g, folium panacis japonici cum caule 65g, cordate houttuynia 30g, bark of eucommia 35g, eclipta 40g, rhizome of davallia 35g, cassia twig 30g, Radix Isatidis 40g, hawthorn 40g, the membrane of a chicken's gizzard 30g, Chinese trumpet creeper 25g, Chinese yam 30g, reticulate millettia 40g, spina gleditsiae 30g, Fructus Corni 35g, peach branch 30g, frutus cnidii 40g, date 35g, tuber of stemona 25g, Gorgon fruit 30g, Galanga Galangal Seed 25g, betel nut 30g, pittosporum illicioides 40g and Horseweed Herb 35g.

The present invention also provides the preparation method of above-mentioned Chinese medicine immune formulation, is specially:

Each bulk drug in Chinese medicine immune formulation is uniformly mixed, send into and in pulverizer, be crushed and screened into 20 order ~ 40 object powder, put into subsequently marmite, the water that adds 4 ~ 6 times of quality, soak 2 ~ 4 hours, then decoct 1 ~ 2 hour, concoction is poured out, again add the water with respect to 2 ~ 4 times of powder qualities, decoct 30 minutes ~ 1 hour, filter, the concoction obtaining and the concoction obtaining above mixing will be filtered, reduced pressure concentration is removed the water of 70% ~ 80% volume, add subsequently ethyl acetate to extract, the ethyl acetate solution of acquisition is carried out to reduced pressure concentration and remove ethyl acetate solvent wherein, subsequent drying, to be dried body again sends into and in pulverizer, pulverizes and be screened into 20 order ~ 40 object powder, with the starch with respect to 3 ~ 5 times of its quality, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese medicine immune formulation.

It is a kind of for improving the feed of grass carp immunologic function that the present invention also provides, and it comprises rape oil, dregs of beans, fish meal, salt, wheat flour, Chinese medicine immune formulation, bacillus subtilis, mineral element additive, vitamin(e) supplement and calcium dihydrogen phosphate.

Wherein, in described feed, the weight of each raw material is respectively rape oil 50g～70g, dregs of beans 900g～1100g, fish meal 50g～70g, salt 10g～20g, wheat flour 200g～300g, Chinese medicine immune formulation 100g～150g, bacillus subtilis 3g～5g, mineral element additive 10g～20g, vitamin(e) supplement 20g～30g, calcium dihydrogen phosphate 5g～10g.

Wherein, in described feed, the weight of each raw material is respectively rape oil 60g, dregs of beans 1000g, fish meal 60g, salt 15g, wheat flour 250g, Chinese medicine immune formulation 130g, bacillus subtilis 4g, mineral element additive 15g, vitamin(e) supplement 25g, calcium dihydrogen phosphate 8g.

The preparation method of the above-mentioned feed that the present invention also provides, is specially:

The first step, each bulk drug in Chinese medicine immune formulation is uniformly mixed, send into and in pulverizer, be crushed and screened into 20 order ~ 40 object powder, put into subsequently marmite, the water that adds 4 ~ 6 times of quality, soak 2 ~ 4 hours, then decoct 1 ~ 2 hour, concoction is poured out, again add the water with respect to 2 ~ 4 times of powder qualities, decoct 30 minutes ~ 1 hour, filter, the concoction obtaining and the concoction obtaining above mixing will be filtered, reduced pressure concentration is removed the water of 70% ~ 80% volume, add subsequently ethyl acetate to extract, the ethyl acetate solution of acquisition is carried out to reduced pressure concentration and remove ethyl acetate solvent wherein, subsequent drying, to be dried body again sends into and in pulverizer, pulverizes and be screened into 20 order ~ 40 object powder, with the starch with respect to 3 ~ 5 times of its quality, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese medicine immune formulation,

Second step, it is even that the Chinese medicine composition that the first step is obtained and other each components in described feed are sent into stirrer for mixing in proportion, and mixture homogeneity is CV%≤10%;

The 3rd step, the material mixing is modulated, and temperature is controlled at 90 ~ 95 ℃, and modulating time 55 ~ 65 seconds is granulated subsequently, and cooling, pellet composition is cooling, and temperature is controlled at 20 ~ 25 ℃, packing.

Beneficial effect of the present invention:

Chinese medicine immune formulation provided by the invention has efficiently, little, safe, the residual plurality of advantages such as few of toxic and side effect, and wide material sources, cost is low, and the immune system of fish is adjusted, improve moving anti-stress ability and the immunity function of aquatic products, thus preventing and curing of fish disease.

 

The specific embodiment

The invention provides a kind ofly for improving the Chinese medicine immune formulation of grass carp immunity function, its bulk drug comprises umbellate pore furgus, wilsonii, the bighead atractylodes rhizome, folium panacis japonici cum caule, cordate houttuynia, the bark of eucommia, eclipta, the rhizome of davallia, cassia twig, Radix Isatidis, hawthorn, the membrane of a chicken's gizzard, Chinese trumpet creeper, Chinese yam, reticulate millettia, spina gleditsiae, Fructus Corni, peach branch, frutus cnidii, date, the tuber of stemona, Gorgon fruit, Galanga Galangal Seed, betel nut, pittosporum illicioides and Horseweed Herb.

Further, described Chinese medicine immune formulation only consists of above-mentioned raw materials medicine.

The pharmacology of each bulk drug is as follows:

Umbellate pore furgus: taste is sweet, light, property is flat, enters kidney, urinary bladder channel, has the effect of clearing damp and promoting diuresis, is mainly used in the oedema of retention of water-damp, difficult urination, and the having loose bowels, vomit of water retention due to hypofunction of the spleen; The cards such as the leukorrhea of damp invasion of lower energizer, gonorrhoea.

Wilsonii: latin name acanthopanacis Senticosi Radix et Rhizomaseu Caulis, taste is pungent, bitter, micro-sweet, warm in nature, enter spleen, kidney, the heart channel of Hang-Shaoyin, have and replenish qi to invigorate the spleen, tonify the kidney to relieve mental strain, the effect of dispelling rheumatism, soft for wind-cold-dampness arthralgia, lumbocrural pain, muscles and bones impotence, be slow in action, the card such as body void is thin and weak, traumatic injury, fracture, oedema, beriberi, uncontrolled urination are itched.

The bighead atractylodes rhizome: latin name attractylodis Macrocephalae Rhizoma, taste is sweet, bitter, warm in nature, enters spleen, stomach warp, has tonifying spleen profit gas, eliminating dampness Li Shui, and strengthening exterior and reducing sweat, the effect that fortune spleen is moisturized, cures mainly ruffian due to the dysfunction of the spleen in transport, QI failing to transform into body fluid and has one's mind filled with and rush down, phlegm and retained fluid oedema; The vexed lazy speech of the deficiency of qi in middle-jiao, metrorrhagia and metrostaxis, proctoptosis with chronic dysentery; The cards such as solid in the playing of deficiency of the spleen causing weakness of QI, abnormal sweating spontaneous perspiration, the bighead atractylodes rhizome can promote gastric secretion, strengthens the absorption of small intestine, and gastrointestinal function is had to regulating action.

Folium panacis japonici cum caule: bitter, sweet, cold in nature, attach to the lung and stomach meridians, there is tonifying Qi, beneficial lung, drives away summer heat, and the effect of promoting the production of body fluid, cures mainly qi-asthenia cough, and hot summer weather is irritated, and Tianjin wound is thirsty, and the head is unclear, the tired weary card that waits of four limbs.

Cordate houttuynia: taste is pungent, cold nature, enters lung, urinary bladder channel, have clearing heat and detoxicating, removing pus and relieving carbuncle, the effect of inducing diuresis for treating strangurtia, for lung carbuncle pyemesis, phlegm heat is coughed and is breathed heavily, toxic-heat and blood stasis, damp-heat gonorrhoea, hot carbuncle carbuncle, snakeworm is wanted first-class card.

The bark of eucommia: taste is sweet, micro-pungent, warm in nature, enters liver, kidney channel, has hypotensive, filling liver kidney, strengthening the bones and muscles, the effect of antiabortive gas, a little less than being shown in dizzy, soreness of waist and knee joint, muscles and bones impotence for caused by liver and kidney deficiency, card; The unconsolidation of the kidney-qi, frequent micturition or dripping urination, uncontrolled urination are itched, impotence, pregnant woman are weak, fetal irritability or waist pendant pain; The cards such as chronic joint disease, bone tuberculosis, dysmenorrhoea, dysfunctional uterine bleeding, chronic pelvic inflammatory disease.

Eclipta: taste is sweet, sour, cold in nature, returns liver, kidney channel, and play and tonify the liver and kidney, the effect of cooling blood and hemostasis, for having a dizzy spell due to the deficiency of liver-yin and kidney-yin, blurring of vision, soreness and weakness of waist and knees; Being suitable for the deficiency of Yin has hot person, is used for the treatment of the haematemesis of accumulated heat in stomach, the cards such as the hemoptysis of heat impairing pulmonary collateral.

The rhizome of davallia: bitter, warm in nature, enter liver, kidney channel, there is nourishing disney and strengthening bone, continuous effect of hindering pain relieving, for the soreness of waist and knee joint of suffering from a deficiency of the kidney, flaccidity and weakness of the lower limbs, the deficiency of liver-yin and kidney-yin, empty positive rising and the deafness and tinnitus that causes, tooth mobility pain or bleeding gums, speen-kidney dificiency and cause and rush down for a long time the cards such as protracted dysentery.

Cassia twig: taste is pungent, sweet, warm in nature, the thoughts of returning home, lung, urinary bladder channel, have sweating expelling pathogenic factors from muscles and skin, warming meridians and promoting circulation of qi, helps yang transforming qi, the effect of eliminating cold to stop pain, cassia twig warming meridians and promoting circulation of qi, horizontal logical podomere, for the illness that has not attacked the vital organs of the human body of catching cold, no matter have sweat, losslessly all can apply, also for arthralgia due to wind-dampness, stomach cold stomachache, through closing, dysmenorrhoea, arthralgia due to wind-dampness, the cards such as shoulder arm podomere crymodynia.

Radix Isatidis: latin name isatidis Radix,bitter, cold in nature, enters the heart, liver, courage, stomach warp, has the effect of clearing heat and detoxicating, cool blood relieve sore throat, is used for the treatment of febrile virulent maculae; High heat headache; Major part pestilence; Tongue is dark reddish purple dark, scarlet fever; Erysipelas; Mumps; Larynx numbness; Sore is swollen, carbuncle is swollen; Varicella; Measles; Hepatitis; Influenza, epidemic meningitis, encephalitis, pneumonia, coma is told nosebleed, and pharynx is swollen, pinkeye, sore rash; Can prevent and treat the cards such as Japanese Type-B encephalitis, acute, chronic hepatitis, mumps, osteomyelitis.

Hawthorn: taste acid, sweet, slightly warm in nature, returns spleen, stomach, Liver Channel, and for food digesting stomach fortifying, the promoting the circulation of qi stasis of blood of fall apart, is mainly used to treat accumulation of food in the stomach and intes tine due to indigestion, have loose bowels, dysentery, hawthorn is the staple for the treatment of indigestion, hawthorn can increase the secretion of peptic digest enzyme, promotes to digest; Shigella dysenteriae, Escherichia coli, Pseudomonas aeruginosa, staphylococcus aureus etc. are had to obvious inhibitory action, and the active ingredient hawthorn fruit total flavone in hawthorn and hawthorn total triterpene acid class have good reducing blood lipid, reduction cholesterol, increase the effect of gastric juice digestive ferment.

The membrane of a chicken's gizzard: taste is sweet, cold in nature, return spleen, stomach, small intestine, urinary bladder channel, have food digesting stomach fortifying, the effect of arresting seminal emission, cures mainly dyspepsia turgor, vomiting gastric disorder causing nausea, rushes down dysentery, infantile malnutrition due to digestive disturbances or intestinalparasites, quench one's thirst, lose and drown, larynx numbness acute tonsillitis, the cards such as noma aphtha, the membrane of a chicken's gizzard has promotion gastric secretion, the effect of gastric acidity and enteral digestion absorbability.

Chinese trumpet creeper: latin name campsis Flos, taste is sweet, sour, cold in nature, returns liver, pericardium channel, and there is promoting circulation of blood and go the stasis of blood, the effect of cooling blood, expelling wind, for Jing Bi wei lump in the abdomen, postpartum, breast was swollen, and rubella is rubescent, pruitus, the cards such as acne.

Chinese yam: taste is sweet, property is put down, enter lung, spleen, kidney channel, have strengthening the spleen and tonifying the lung, beneficial stomach kidney tonifying, the benefit of reinforcing the kidney essence, hearing-improing and eyesight improving, the effect that helps the five internal organs, strengthening the bones and muscles, long will to calm the nerves, promote longevity, cure mainly that weakness of the spleen and the stomach, lassitude, poor appetite, chronic diarrhea and dysentery, Lung Qi deficiency are dry, asthma due to excessive phlegm cough, kidney qi loss, soreness and weakness of waist and knees, lower limb impotence are weak, the red card such as swollen, fat of gonorrhoea under the frequent micturition of quenching one's thirst, seminal emission premature ejaculation, band, skin.

Reticulate millettia: bitter, sweet, warm in nature, return liver, kidney channel, there is hemangiectasis, enrich blood and invigorate blood circulation, menstruation regulating, the effect of stimulating the circulation of the blood and cause the muscles and joints to relax, cures mainly the irregular menstruation that the deficiency of blood has silt, and limbs pain is meciless, the cards such as soreness and weakness of waist and knees, pain.

Spina gleditsiae: taste is pungent, warm in nature, return lung channel, cure mainly swollen ulcer drug, scrofula, sore rash stubborn dermatitis, hypogalactia after delivery, retention of afterbirth, the cards such as pestilence wind.

Fructus Corni: taste acid, puckery, slightly warm in nature, returns liver, kidney channel, has and tonifies the liver and kidney, the effect that puckery essence is admittedly de-.For dizzy tinnitus, soreness of waist and knee joint, impotence and seminal emission, enuresis frequent micturition, under uterine bleeding band, profuse sweating collapse.The interior heat card such as quench one's thirst.

Peach branch: latin name persicae Ramulus, bitter, property is flat, and the thoughts of returning home, Liver Channel, have activating blood to promote menstruation, and the effect of removing toxic substances desinsection, for trusted subordinate's shouting pain, arthralgia due to wind-dampness, the cards such as traumatic injury.

Frutus cnidii: taste is pungent, bitter, warm in nature, enters kidney channel, the effect of there is warming kidney to invigorate yang, removing dampness and destroying parasites, dispellingging wind and arresting itching, the impotence in male causing for decline of kidney-YANG, woman Gong Leng are infertile, under cold-dampness band or the card such as waist knee crymodynia.

Date: taste is sweet, warm in nature, enter spleen, stomach warp, there is the effect of air making-up and spleen enlivening, nourishing blood and tranquilization, harmonizing yingfen and weifen, the mitigation property of medicine, for weakness of the spleen and the stomach, fatigue and asthenia, anorexia and loose stool, the card such as insufficiency of vital energy and blood, has a headache and fever, sweating hot blast.

The tuber of stemona: taste is sweet, bitter, slightly warm in nature enters lung channel, have and moisten the lung and relieve the cough, the effect of delousing desinsection, tuber of stemona temperature embellishes but not dry, and property is kept and is not walked, the merit that has out again drop-down gas, be mainly used in new all coughing for a long time, be especially suitable for the hot chronic cough person of labor, the modern times are used for preventing and treating the cough of chronic bronchitis, the dry cough of pulmonary tuberculosis, the cards such as pertussis.

Gorgon fruit: taste is sweet, puckery, property is flat.Return spleen, kidney channel.There is supplementing the kidney to control the nocturnal, tonifying spleen antidiarrheal, clearing damp is the function of band only, cures mainly emission, splenasthenic diarrhea, insufficiency of the spleen gonorrhoea, is with inferior card.

Galanga Galangal Seed: latin name hedysari Radix, taste is pungent, warm in nature, returns spleen, lung channel, has eliminating dampness in temperature, and eliminating dampness is loose cold, and the effect of being amusing and helping digestion cures mainly the cards such as coldness and pain in the epigastrium, dyspepsia turgor, vomiting are had loose bowels, drinking too much.

Betel nut: bitter, pungent, warm in nature, return stomach, large intestine channel, there is desinsection, disappear long-pending, promoting the circulation of qi, Li Shui, the effect of preventing malaria, cures mainly abdominal pain due to enterositosis, the stagnant dysentery of rushing down, tenesmus, oedema beriberi, the cards such as malaria, betel nut can promote stomach motion, the secretion that improves motilin and vasoactive intestinal peptide.

Pittosporum illicioides: bitter, pungent, warm in nature, there is activating collaterals to relieve pain, heart-calming kidney-nourishing, the effect of removing toxic substances, cures mainly arthralgia due to wind-dampness, fracture, stomachache, insomnia, seminal emission, the cards such as venomous snake bite.

Horseweed Herb: mildly bitter flavor, pungent, cool in nature, there is clearing heat and promoting diuresis, the effect of removing toxicity for detumescence, cures mainly dysentery, enteritis, hepatitis, cholecystitis, tympanitis, conjunctivitis, traumatic injury, treating rheumatic ostealgia, traumatism and bleeding, the cards such as eczema.

Wherein, in described Chinese medicine immune formulation, the weight of each bulk drug is respectively umbellate pore furgus 25g～35g, wilsonii 45g～55g, bighead atractylodes rhizome 35g～45g, folium panacis japonici cum caule 60g～70g, cordate houttuynia 25g～35g, bark of eucommia 30g～40g, eclipta 35g～45g, rhizome of davallia 30g～40g, cassia twig 25g～35g, Radix Isatidis 35g～45g, hawthorn 35g～45g, the membrane of a chicken's gizzard 25g～35g, Chinese trumpet creeper 20g～30g, Chinese yam 25g～35g, reticulate millettia 35g～45g, spina gleditsiae 25g～35g, Fructus Corni 30g～40g, peach branch 25g～35g, frutus cnidii 35g～45g, date 30g～40g, tuber of stemona 20g～30g, Gorgon fruit 25g～35g, Galanga Galangal Seed 20g～30g, betel nut 25g～35g, pittosporum illicioides 35g～45g and Horseweed Herb 30g～40g.

Wherein, in described Chinese medicine immune formulation, the weight of each bulk drug is respectively umbellate pore furgus 30g, wilsonii 50g, bighead atractylodes rhizome 40g, folium panacis japonici cum caule 65g, cordate houttuynia 30g, bark of eucommia 35g, eclipta 40g, rhizome of davallia 35g, cassia twig 30g, Radix Isatidis 40g, hawthorn 40g, the membrane of a chicken's gizzard 30g, Chinese trumpet creeper 25g, Chinese yam 30g, reticulate millettia 40g, spina gleditsiae 30g, Fructus Corni 35g, peach branch 30g, frutus cnidii 40g, date 35g, tuber of stemona 25g, Gorgon fruit 30g, Galanga Galangal Seed 25g, betel nut 30g, pittosporum illicioides 40g and Horseweed Herb 35g.

The present invention also provides the preparation method of above-mentioned Chinese medicine immune formulation, is specially:

Each bulk drug in Chinese medicine immune formulation is uniformly mixed, send into and in pulverizer, be crushed and screened into 20 order ~ 40 object powder, put into subsequently marmite, the water that adds 4 ~ 6 times of quality, soak 2 ~ 4 hours, then decoct 1 ~ 2 hour, concoction is poured out, again add the water with respect to 2 ~ 4 times of powder qualities, decoct 30 minutes ~ 1 hour, filter, the concoction obtaining and the concoction obtaining above mixing will be filtered, reduced pressure concentration is removed the water of 70% ~ 80% volume, add subsequently ethyl acetate to extract, the ethyl acetate solution of acquisition is carried out to reduced pressure concentration and remove ethyl acetate solvent wherein, subsequent drying, to be dried body again sends into and in pulverizer, pulverizes and be screened into 20 order ~ 40 object powder, with the starch with respect to 3 ~ 5 times of its quality, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese medicine immune formulation.

It is a kind of for improving the feed of grass carp immunologic function that the present invention also provides, and it comprises rape oil, dregs of beans, fish meal, salt, wheat flour, above-mentioned Chinese medicine immune formulation, bacillus subtilis, mineral element additive, vitamin(e) supplement and calcium dihydrogen phosphate.

Further, described feed only consists of above-mentioned raw materials.

Wherein, in described feed, the weight of each raw material is respectively rape oil 50g～70g, dregs of beans 900g～1100g, fish meal 50g～70g, salt 10g～20g, wheat flour 200g～300g, Chinese medicine immune formulation 100g～150g, bacillus subtilis 3g～5g, mineral element additive 10g～20g, vitamin(e) supplement 20g～30g, calcium dihydrogen phosphate 5g～10g.

Wherein, in described feed, the weight of each raw material is respectively rape oil 60g, dregs of beans 1000g, fish meal 60g, salt 15g, wheat flour 250g, Chinese medicine immune formulation 130g, bacillus subtilis 4g, mineral element additive 15g, vitamin(e) supplement 25g, calcium dihydrogen phosphate 8g.

Described vitamin(e) supplement can be various vitamin, as vitamin A, vitamin C, vitamin E etc., preferred vitamin premix, described vitamin premix can be FV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production), and it contains vitamin C is that 100g/kg, vitamin E are 50,000 IU/kg.

Mineral element has a very important role to the immunity function of aquatic livestock, it is by acting on the immune organ of aquatic livestock and affecting its immunologic function and health status with factors such as Ia enzymes, mineral element in feed and water body lacks or when excessive, the immunologic function of capital infringement aquatic livestock, increases its neurological susceptibility to disease.Therefore, in aquatic livestock, the appropriate mineral element that uses can play promotion growth, improve the effect of immunity and resistance against diseases, mineral substance premix provided by the present invention can be to contain zinc, iron, copper, the compound of the trace element such as manganese or the mixture of amino-acid chelate, also can select the existing mineral substance premix additive that can buy on the market, as the micro-composite trace element premix additive of excellent profit of Beijing You Libao Bioisystech Co., Ltd production, its main component is ferrous sulfate, Copper lysinate, zinc methionine, manganese sulfate, sodium selenite, Potassiumiodate, cobalt chloride etc., carrier is medical stone, wherein in every kg additive, iron content is 30g, copper content 17g, zinc content 30g, manganese content 20g.

The preparation method of the above-mentioned feed that the present invention also provides, is specially:

The first step, each bulk drug in Chinese medicine immune formulation is uniformly mixed, send into and in pulverizer, be crushed and screened into 20 order ~ 40 object powder, put into subsequently marmite, the water that adds 4 ~ 6 times of quality, soak 2 ~ 4 hours, then decoct 1 ~ 2 hour, concoction is poured out, again add the water with respect to 2 ~ 4 times of powder qualities, decoct 30 minutes ~ 1 hour, filter, the concoction obtaining and the concoction obtaining above mixing will be filtered, reduced pressure concentration is removed the water of 70% ~ 80% volume, add subsequently ethyl acetate to extract, the ethyl acetate solution of acquisition is carried out to reduced pressure concentration and remove ethyl acetate solvent wherein, subsequent drying, to be dried body again sends into and in pulverizer, pulverizes and be screened into 20 order ~ 40 object powder, with the starch with respect to 3 ~ 5 times of its quality, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese medicine immune formulation,

Second step, it is even that the Chinese medicine composition that the first step is obtained and other each components in described feed are sent into stirrer for mixing in proportion, and mixture homogeneity is CV%≤10%;

The 3rd step, the material mixing is modulated, and temperature is controlled at 90 ~ 95 ℃, and modulating time 55 ~ 65 seconds is granulated subsequently, and cooling, pellet composition is cooling, and temperature is controlled at 20 ~ 25 ℃, packing.

Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure of reaching technique effect can fully understand and implement according to this.

The preparation of embodiment 1 Chinese medicine immune formulation 1

By umbellate pore furgus 30g, wilsonii 50g, bighead atractylodes rhizome 40g, folium panacis japonici cum caule 65g, cordate houttuynia 30g, bark of eucommia 35g, eclipta 40g, rhizome of davallia 35g, cassia twig 30g, Radix Isatidis 40g, hawthorn 40g, the membrane of a chicken's gizzard 30g, Chinese trumpet creeper 25g, Chinese yam 30g, reticulate millettia 40g, spina gleditsiae 30g, Fructus Corni 35g, peach branch 30g, frutus cnidii 40g, date 35g, tuber of stemona 25g, Gorgon fruit 30g, Galanga Galangal Seed 25g, betel nut 30g, pittosporum illicioides 40g and Horseweed Herb 35g are uniformly mixed, send into and in pulverizer, be crushed and screened into 40 object powder, put into subsequently marmite, the water that adds 4.5kg, soak 3 hours, then decoct 2 hours, concoction is poured out, the water that again adds 2.5kg, decoct 1 hour, filter, the concoction obtaining and the concoction obtaining above mixing will be filtered, reduced pressure concentration is removed the water of 80% volume, add subsequently ethyl acetate to extract, the ethyl acetate solution of acquisition is carried out to reduced pressure concentration and remove ethyl acetate solvent wherein, subsequent drying, to be dried body again sends into and in pulverizer, pulverizes and be screened into 20 object powder, with the starch with respect to 4 times of its quality, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese medicine immune formulation 1.

The preparation of embodiment 2 Chinese medicine immune formulations 2

By 25g, wilsonii 45g, bighead atractylodes rhizome 35g, folium panacis japonici cum caule 60g, cordate houttuynia 25g, bark of eucommia 30g, eclipta 35g, rhizome of davallia 30g, cassia twig 25g, Radix Isatidis 35g, hawthorn 35g, the membrane of a chicken's gizzard 25g, Chinese trumpet creeper 20g, Chinese yam 25g, reticulate millettia 35g, spina gleditsiae 25g, Fructus Corni 30g, peach branch 25g, frutus cnidii 35g, date 30g, tuber of stemona 20g, Gorgon fruit 25g, Galanga Galangal Seed 20g, betel nut 25g, pittosporum illicioides 35g and Horseweed Herb 30g are uniformly mixed, send into and in pulverizer, be crushed and screened into 40 object powder, put into subsequently marmite, the water that adds 4kg, soak 3 hours, then decoct 2 hours, concoction is poured out, the water that again adds 2.2kg, decoct 1 hour, filter, the concoction obtaining and the concoction obtaining above mixing will be filtered, reduced pressure concentration is removed the water of 80% volume, add subsequently ethyl acetate to extract, the ethyl acetate solution of acquisition is carried out to reduced pressure concentration and remove ethyl acetate solvent wherein, subsequent drying, to be dried body again sends into and in pulverizer, pulverizes and be screened into 20 object powder, with the starch with respect to 4 times of its quality, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese medicine immune formulation 2.

The preparation of embodiment 3 Chinese medicine immune formulations 3

By umbellate pore furgus 35g, wilsonii 55g, bighead atractylodes rhizome 45g, folium panacis japonici cum caule 70g, cordate houttuynia 35g, bark of eucommia 40g, eclipta 45g, rhizome of davallia 40g, cassia twig 35g, Radix Isatidis 45g, hawthorn 45g, the membrane of a chicken's gizzard 35g, Chinese trumpet creeper 30g, Chinese yam 35g, reticulate millettia 45g, spina gleditsiae 35g, Fructus Corni 40g, peach branch 35g, frutus cnidii 45g, date 40g, tuber of stemona 30g, Gorgon fruit 35g, Galanga Galangal Seed 30g, betel nut 35g, pittosporum illicioides 45g and Horseweed Herb 40g are uniformly mixed, send into and in pulverizer, be crushed and screened into 40 object powder, put into subsequently marmite, the water that adds 5kg, soak 3 hours, then decoct 2 hours, concoction is poured out, the water that again adds 3kg, decoct 1 hour, filter, the concoction obtaining and the concoction obtaining above mixing will be filtered, reduced pressure concentration is removed the water of 80% volume, add subsequently ethyl acetate to extract, the ethyl acetate solution of acquisition is carried out to reduced pressure concentration and remove ethyl acetate solvent wherein, subsequent drying, to be dried body again sends into and in pulverizer, pulverizes and be screened into 20 object powder, with the starch with respect to 4 times of its quality, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese medicine immune formulation 3.

Embodiment 4 feeds 1

By rape oil 60g, dregs of beans 1000g, fish meal 60g, salt 15g, wheat flour 250g, the Chinese medicine immune formulation 130g of embodiment 1 preparation, bacillus subtilis 4g, the excellent profit of mineral element additive micro-(production of Beijing You Libao Bioisystech Co., Ltd) 15g, vitamin(e) supplement FV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production) 25g, it is even that calcium dihydrogen phosphate 8g sends into stirrer for mixing, mixture homogeneity is CV%≤10%, the material mixing is modulated, temperature is controlled at 90 ~ 95 ℃, modulating time 55 ~ 65 seconds, granulate subsequently, cooling, pellet composition is cooling, temperature is controlled at 20 ~ 25 ℃, packing, obtain feed 1.

Comparative example 1 feed 2

By rape oil 60g, dregs of beans 1000g, fish meal 60g, salt 15g, wheat flour 250g, bacillus subtilis 4g, the excellent profit of mineral element additive micro-(production of Beijing You Libao Bioisystech Co., Ltd) 15g, vitamin(e) supplement FV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production) 25g, it is even that calcium dihydrogen phosphate 8g sends into stirrer for mixing, mixture homogeneity is CV%≤10%, the material mixing is modulated, temperature is controlled at 90 ~ 95 ℃, modulating time 55 ~ 65 seconds, granulate subsequently, cooling, pellet composition is cooling, temperature is controlled at 20 ~ 25 ℃, packing, obtain feed 2.

Effect test

Test fish

Test uses grass carp from farm, Qingdao, after supporting temporarily, therefrom select fish 750 tails (average body quality 43g) of neat specification, physique stalwartness, with after 3% common salt aqueous solution sterilization, be divided at random 30 groups (every group of 25 tails), every group of 3 repetitions, put into 30 (1.0m * 1.0m * 0.5m) net cages.If 3 control groups, remain 7 for effect group.Net cage is placed in the cement pit of 7.5m * 7.5m * 1.5m.

Test feed

The feed 1 of test group feeding embodiment 4 preparations, the feed 2 of control group feeding comparative example 1 preparation.

Feeding and management

Duration of test, 17～25 ℃ of water temperatures.Throw something and feed when 9:00 and during 16:00 every day, and feeding rate is weight 5%, according to water temperature, the situation of ingesting adjustment.Blowdown every day, change water 1 time, whole day inflation, dissolved oxygen remains on that 5 mg/L are above, NH3-N<0.3 mg/L.

Sero-immunity index determining

Every case is got 5 tail fishes, tail vein blood, and the centrifugal 5min of 3000 r/min, gets serum standby.Superoxide dismutase (SOD), catalase (CAT), alkaline phosphatase (AKP) be active all adopts the assay method that builds up the kit that biotechnology research produces by Nanjing to measure.

Data analysis and processing

Result of the test represents with mean value ± standard error, adopts SPSS13.0 statistical software to carry out one-way analysis of variance to test data and Dun-can ' s multiple ratio is carried out statistical analysis, and the level of signifiance is 0.05.

As a result, in Table 1.

The impact of table 1 Chinese medicine immune formulation on Grass Carp Serum SOD, CAT and AKP activity

Group	SOD(U·mL - 1)	CAT(U·mL - 1)	AKP (the 100mL of King unit - 1)
				Control group	113.91±1.39	1.73±0.19	7.17±0.39
Effect group	135.86±1.21	2.62±0.21	9.23±0.26

SOD is one of antioxidase crucial in Fish, it is the natural remover of superoxide radical, can remove unnecessary free radical in body, exempt it to damage of biomolecule etc., therefore the immune level of SOD and organism is closely related, to strengthening the immunologic function of cytophagous defence capability and body, there is important function, as can be seen from Table 1, adopt Chinese medicine immune formulation provided by the invention can improve the SOD level in grass carp body, CAT is the important antioxidant enzyme of removing body oxygen radical, in healthy fish body, in its interior environment, the generation of free radical and elimination are in dynamic equilibrium.When CAT activity decreased, fish interior free yl too much can destroy some important biochemical processes in body, cause metabolism chaotic, normal physiological function imbalance, in body, immune level declines, potential cause of disease is activated, make the morbidity of fish body, as can be seen from Table 1, adopt Chinese medicine immune formulation provided by the invention can improve the CAT level in grass carp body, AKP is a kind of important hydrolase, the hydrolysis of energy catalysis phosphoric acid monoester and the transfer reaction of phosphate group, digestion degraded to foreign matters such as bacteriums in lysosome has important function, and play a role in immune response, existence to animal has great importance, AKP is used as an important immune indexes and is widely studied in animal immunology all the time, as can be seen from Table 1, adopt Chinese medicine immune formulation provided by the invention can improve the AKP level in grass carp body, therefore, adopt immune formulation of the present invention can significantly improve the immunologic function of grass carp.

All above-mentioned these intellectual properties of primary enforcement, do not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.

The above, be only preferred embodiment of the present invention, is not the present invention to be done to the restriction of other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not depart from, any simple modification, equivalent variations and the remodeling above embodiment done according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.

Claims (7)

1. for improving a feed for grass carp immunologic function, it is characterized in that: comprise rape oil, dregs of beans, fish meal, salt, wheat flour, Chinese medicine immune formulation, bacillus subtilis, mineral element additive, vitamin(e) supplement and calcium dihydrogen phosphate.

2. feed as claimed in claim 1, is characterized in that: in described feed, the weight of each raw material is respectively rape oil 50g～70g, dregs of beans 900g～1100g, fish meal 50g～70g, salt 10g～20g, wheat flour 200g～300g, Chinese medicine immune formulation 100g～150g, bacillus subtilis 3g～5g, mineral element additive 10g～20g, vitamin(e) supplement 20g～30g, calcium dihydrogen phosphate 5g～10g.

3. feed as claimed in claim 1 or 2, is characterized in that: in described feed, the weight of each raw material is respectively rape oil 60g, dregs of beans 1000g, fish meal 60g, salt 15g, wheat flour 250g, Chinese medicine immune formulation 130g, bacillus subtilis 4g, mineral element additive 15g, vitamin(e) supplement 25g, calcium dihydrogen phosphate 8g.

4. the feed as described in claims 1 to 3, is characterized in that: the bulk drug of described Chinese medicine immune formulation comprises umbellate pore furgus, wilsonii, the bighead atractylodes rhizome, folium panacis japonici cum caule, cordate houttuynia, the bark of eucommia, eclipta, the rhizome of davallia, cassia twig, Radix Isatidis, hawthorn, the membrane of a chicken's gizzard, Chinese trumpet creeper, Chinese yam, reticulate millettia, spina gleditsiae, Fructus Corni, peach branch, frutus cnidii, date, the tuber of stemona, Gorgon fruit, Galanga Galangal Seed, betel nut, pittosporum illicioides and Horseweed Herb.

5. the feed as described in claim 1 to 4, it is characterized in that: the weight of described each bulk drug of Chinese medicine immune formulation is respectively umbellate pore furgus 25g～35g, wilsonii 45g～55g, bighead atractylodes rhizome 35g～45g, folium panacis japonici cum caule 60g～70g, cordate houttuynia 25g～35g, bark of eucommia 30g～40g, eclipta 35g～45g, rhizome of davallia 30g～40g, cassia twig 25g～35g, Radix Isatidis 35g～45g, hawthorn 35g～45g, the membrane of a chicken's gizzard 25g～35g, Chinese trumpet creeper 20g～30g, Chinese yam 25g～35g, reticulate millettia 35g～45g, spina gleditsiae 25g～35g, Fructus Corni 30g～40g, peach branch 25g～35g, frutus cnidii 35g～45g, date 30g～40g, tuber of stemona 20g～30g, Gorgon fruit 25g～35g, Galanga Galangal Seed 20g～30g, betel nut 25g～35g, pittosporum illicioides 35g～45g and Horseweed Herb 30g～40g.

6. the feed as described in claim 1 to 5, is characterized in that: the weight of described each bulk drug of Chinese medicine immune formulation is respectively umbellate pore furgus 30g, wilsonii 50g, bighead atractylodes rhizome 40g, folium panacis japonici cum caule 65g, cordate houttuynia 30g, bark of eucommia 35g, eclipta 40g, rhizome of davallia 35g, cassia twig 30g, Radix Isatidis 40g, hawthorn 40g, the membrane of a chicken's gizzard 30g, Chinese trumpet creeper 25g, Chinese yam 30g, reticulate millettia 40g, spina gleditsiae 30g, Fructus Corni 35g, peach branch 30g, frutus cnidii 40g, date 35g, tuber of stemona 25g, Gorgon fruit 30g, Galanga Galangal Seed 25g, betel nut 30g, pittosporum illicioides 40g and Horseweed Herb 35g.

7. the preparation method of feed described in claim 1 to 6, is characterized in that, comprising:

The first step, each bulk drug in Chinese medicine immune formulation is uniformly mixed, send into and in pulverizer, be crushed and screened into 20 order ~ 40 object powder, put into subsequently marmite, the water that adds 4 ~ 6 times of quality, soak 2 ~ 4 hours, then decoct 1 ~ 2 hour, concoction is poured out, again add the water with respect to 2 ~ 4 times of powder qualities, decoct 30 minutes ~ 1 hour, filter, the concoction obtaining and the concoction obtaining above mixing will be filtered, reduced pressure concentration is removed the water of 70% ~ 80% volume, add subsequently ethyl acetate to extract, the ethyl acetate solution of acquisition is carried out to reduced pressure concentration and remove ethyl acetate solvent wherein, subsequent drying, to be dried body again sends into and in pulverizer, pulverizes and be screened into 20 order ~ 40 object powder, with the starch with respect to 3 ~ 5 times of its quality, mix, with 14 mesh sieves, granulate, in 60 ℃ ~ 70 ℃ baking ovens, toast 30 minutes, obtain Chinese medicine immune formulation,

Second step, it is even that the Chinese medicine composition that the first step is obtained and other each components in described feed are sent into stirrer for mixing in proportion, and mixture homogeneity is CV%≤10%;

The 3rd step, the material mixing is modulated, and temperature is controlled at 90 ~ 95 ℃, and modulating time 55 ~ 65 seconds is granulated subsequently, and cooling, pellet composition is cooling, and temperature is controlled at 20 ~ 25 ℃, packing.