CN106578657A - Grass carp Chinese medicinal feed additive and preparation method thereof - Google Patents

Grass carp Chinese medicinal feed additive and preparation method thereof Download PDF

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Publication number
CN106578657A
CN106578657A CN201610918111.XA CN201610918111A CN106578657A CN 106578657 A CN106578657 A CN 106578657A CN 201610918111 A CN201610918111 A CN 201610918111A CN 106578657 A CN106578657 A CN 106578657A
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parts
radix
feed additive
fructus
chinese medicine
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黄家绍
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Nanning Sea World Biotechnology Co Ltd
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Nanning Sea World Biotechnology Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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Abstract

Belonging to the technical field of feed additive research and development, the invention in particular relates to a grass carp Chinese medicinal feed additive and a preparation method thereof. The grass carp Chinese medicinal feed additive is prepared from the following components by weight: 20-30 parts of rhizoma drynariae, 10-30 parts of Chinese angelica, 8-20 parts of Taxillus sutchuenensis, 10-20 parts of prepared dried ginger, 8-16 parts of Himalayan teasel root, 8-20 parts of Schisandra chinensis, 9-18 parts of fructus forsythia, 10-30 parts of psoralea corylifolia, 9-20 parts of cortex lycii, 7-21 parts of chrysanthemum, 25-35 parts of morinda officinalis, 15-30 parts of Saposhnikovia divaricata, 15-30 parts of Bulbus Fritillaria Thunbergii, 10-19 parts of as arum, 8-15 parts of muscardine silkworm, 9-20 parts of mulberry twig, 25-35 parts of trogopterus dung, 25-35 parts of radix bupleuri, 8-15 parts of phellodendron, 9-16 parts of platycodon grandiflorus, 10-25 parts of fructus aurantii immaturus, 10-20 parts of plantain seed, and 6-15 parts of fructus forsythia. All Chinese herbal ingredients of the grass carp Chinese medicinal feed additive have a synergetic effect, the prepared Chinese medicinal feed additive can prevent grass carp from suffering illness, increase the grass carp survival rate, and improve the activity level of lysozyme, superoxide dismutase, catalase and alkaline phosphatase in grass carp.

Description

A kind of Ctenopharyngodon idellus Chinese medicine feed additive and preparation method thereof
Technical field
The invention belongs to feed additive research and development technology field, more particularly to a kind of Ctenopharyngodon idellus Chinese medicine feed additive and its system Preparation Method.
Background technology
Ctenopharyngodon idellus are one of large Chinese carps of China four, are also the main Freshwater Fish of China, Ctenopharyngodon idellus cultivation have feed cost it is low, The advantages of economic benefit is obvious.But erythroderma, gill rot, enteritis disease and the bleeding being easily subject in breeding process due to Ctenopharyngodon idellus Disease is referred to as the infringement of " Ctenopharyngodon idellus four are sick ", and fish production is made a big impact.Problem is prevented and treated in order to solve Ctenopharyngodon idellus " four is sick ", Need the immune function for improving Ctenopharyngodon idellus conscientiously.
Chinese herbal medicine is a kind of preferably natural, environment-friendly medicine, little, safe, residual with efficient, toxic and side effects Many advantages, such as staying few, and wide material sources, low cost.The immunity of aquatic animal is improved by the administration of Chinese herbal medicine, fish is improved Class premunition, the sickness rate for reducing aquatic animal is the focus studied in recent years.In the new shape for advocating healthy aquaculture comprehensively at present Under gesture, fish immunity function is improved using Chinese herbal medicine, be extremely important.
Application No. 201310630290.3《For improving the immune formulation of Ctenopharyngodon idellus immune function》Patent of invention it is public Open a kind of immune formulation for improving Ctenopharyngodon idellus immune function, it is characterised in that:Crude drug is by Polyporus, Radix Et Caulis Acanthopanacis Senticosi, the Rhizoma Atractylodis Macrocephalae, people Rhizoma panacis majoris, Herba Houttuyniae, the Cortex Eucommiae, Herba Ecliptae, Rhizoma Drynariae, Ramulus Cinnamomi, Radix Isatidis, Fructus Crataegi, Endothelium Corneum Gigeriae Galli, Flos Campsiss, Rhizoma Dioscoreae, Caulis Spatholobi, soap Fructus Gleditsia acupuncture, Fructus Corni, Ramulus Persicae, Fructus Cnidii, Fructus Jujubae, the Radix Stemonae, Semen Euryaless, Fructus Galangae, Semen Arecae, pittosporm root and Conyza canadensis (L.) Cronq. are constituted.From entirety See, the formula biases toward spleen reinforcing and supports kidney, adjusts gastrointestinal function, for enteritis disease effect still, but the patent is not given enough Data illustrate that this kind of immune formulation has ideal effect for the preventing and treating of Ctenopharyngodon idellus viral disease, bacterial disease.
In view of the defect of prior art, a kind of Chinese medicine fodder addition for improving Ctenopharyngodon idellus immune function of present invention proposition Agent, its have efficiently, have no toxic side effect, safe, noresidue many advantages, such as, and wide material sources, low cost, to Fish Immune system carries out across-the-board regulation, the dynamic resistances against diseases of Aquatic product and immune function is improved, so as to preventing and curing of fish disease.
The information for being disclosed in the background section is merely intended to increase the understanding of the general background to the present invention, and should not When the prior art for being considered to recognize or imply in any form the information structure well known to persons skilled in the art.
The content of the invention
For above-mentioned deficiency present in prior art, the present invention provides a kind of Ctenopharyngodon idellus Chinese medicine feed additive and its preparation Method, the Chinese medicine feed additive reasonable recipe, prepare simple, effect substantially, have no toxic side effect, have no drug resistance it is residual with medicine Stay, Ctenopharyngodon idellus immune function can be effectively improved.
To achieve these goals, the present invention is to employ the following technical solutions realization:
A kind of Ctenopharyngodon idellus Chinese medicine feed additive, by being made up of following components by weight:Rhizoma Drynariae 20-30 parts, Radix Angelicae Sinensis 10- 30 parts, Herba Taxilli 8-20 parts, Rhizoma Zingiberis Preparatum 10-20 parts, Radix Dipsaci 8-16 parts, Fructus Schisandrae Chinensis 8-20 parts, Fructus Forsythiae 9-18 parts, Fructus Psoraleae 10-30 Part, Cortex Lycii 9-20 parts, Flos Chrysanthemi 7-21 parts, Morinda offcinalis How 25-35 part, Radix Saposhnikoviae 15-30 parts, Bulbus Fritillariae Thunbergii 15-30 part, Herba Asari 10-19 parts, deadlock Silkworm 8-15 parts, Ramulus Mori 9-20 parts, Oletum Trogopterori 25-35 parts, Radix Bupleuri 25-35 parts, Cortex Phellodendri 8-15 parts, Radix Platycodoniss 9-16 parts, Fructus Aurantii Immaturus 10-25 Part, Semen Plantaginiss 10-20 parts and Fructus Forsythiae 6-15 parts.
Preferably, described Ctenopharyngodon idellus Chinese medicine feed additive, by being made up of following components by weight:Rhizoma Drynariae 25 Part, 20 parts of Radix Angelicae Sinensis, 12 parts of Herba Taxilli, 15 parts of Rhizoma Zingiberis Preparatum, 12 parts of Radix Dipsaci, 14 parts of Fructus Schisandrae Chinensis, 15 parts of Fructus Forsythiae, 25 parts of Fructus Psoraleae, bone 15 parts of skin, 12 parts of Flos Chrysanthemi, 30 parts of Morinda offcinalis How, 20 parts of Radix Saposhnikoviae, 25 parts of Bulbus Fritillariae Thunbergii, 15 parts of Herba Asari, 11 parts of Bombyx Batryticatus, 15 parts of Ramulus Mori, Oletum Trogopterori 10 parts of 30 parts, 30 parts of Radix Bupleuri, 12 parts of Cortex Phellodendri, 12 parts of Radix Platycodoniss, 20 parts of Fructus Aurantii Immaturus, 15 parts of Semen Plantaginiss and Fructus Forsythiae.
In addition, present invention also offers the preparation method of described Ctenopharyngodon idellus Chinese medicine feed additive, comprises the following steps:
(1) Rhizoma Drynariae, Radix Angelicae Sinensis, Herba Taxilli, Rhizoma Zingiberis Preparatum, Radix Dipsaci, Fructus Schisandrae Chinensis, Fructus Forsythiae, the benefit in formula is weighed by weight Bone fat, Cortex Lycii, Flos Chrysanthemi, Morinda offcinalis How, Radix Saposhnikoviae, Bulbus Fritillariae Thunbergii, Herba Asari, Bombyx Batryticatus, Ramulus Mori, Oletum Trogopterori, Radix Bupleuri, Cortex Phellodendri, Radix Platycodoniss, Fructus Aurantii Immaturus, car Front son, Fructus Forsythiae, clean and dry, and in addition to Herba Asari, Morinda offcinalis How, after being pulverized and mixed by pulverizer, add its 3-4 times of weight originally Water, first boils again slow fire by intense fire and endures 0.5-1h, and first filtrate is obtained after filtration, and toward medicinal residues the tap water of its weight 2-3 times is added, military Fire boils rear slow fire to be endured 0.5h and adds that Herba Asari, Morinda offcinalis How are continuous to endure 10-15min, pours out second filtrate, merges dense after filtrate twice Contract to obtain herb liquid study;
(2) dehydrated alcohol of its volume 3-5 times, stirring and dissolving is added to dilute in the herb liquid study obtained by step (1), Flow back 2.5-3.5h at a temperature of 85-95 DEG C, obtains refined herb liquid;
(3) the refined herb liquid obtained by step (2) is taken, after decompression recycling ethanol, by herb liquid low-temperature evaporation, latex shape is obtained Middle medicinal substances, glue milky substance are put in drying machine and are dried, and inlet temperature is 130-150 DEG C, and leaving air temp is 75-90 DEG C, Powdered rubber is obtained, powdered rubber is crushed to into 120 mesh, obtain Chinese medicinal powder;
(4) Chinese medicinal powder obtained by step (3) is admixed the Semen Maydis powder of its quality 2-3 times, is mixed, carry out expanded, temperature At 85-90 DEG C, expanded time 1-2min, subsequent pelletize, cooling treats that particulate component is cooled to 20-25 DEG C, and packaging is obtained final product for control Ctenopharyngodon idellus Chinese medicine feed additive.
Compared with prior art, the present invention has the advantages that:
(1) there is synergistic function, in preparing between each Chinese medicine ingredients of Ctenopharyngodon idellus Chinese medicine feed additive of the invention Medicine feed additive has functions that kidney, digestion promoting are supported in convergence removing toxic substances, spleen invigorating, and the immune system of Fish is adjusted, and improves The anti-stress ability and immune function of Ctenopharyngodon idellus, is prevented from Ctenopharyngodon idellus illness, improves Ctenopharyngodon idellus survival rate, improves bacteriolyze in Ctenopharyngodon idellus body Enzyme, superoxide dismutase, catalase, the activity level of alkali phosphatase.
(2) Ctenopharyngodon idellus Chinese medicine feed additive raw material of the invention is pure natural raw material, without any chemical classes medicine, system Preparation Method is simple, and the Chinese medicine feed additive residual rate is low, toxicity is low, does not affect the quality of Ctenopharyngodon idellus, is not detrimental to health.
(3) Ctenopharyngodon idellus Chinese medicine feed additive preparation method of the invention is simple, it is easy to large-scale promotion and application.
Specific embodiment
The specific embodiment of the present invention is described in detail with reference to specific embodiment, it is to be understood that of the invention Protection domain do not limited by specific embodiment.
Embodiment 1
A kind of Ctenopharyngodon idellus Chinese medicine feed additive, by being made up of following components by weight:20 parts of Rhizoma Drynariae, 10 parts of Radix Angelicae Sinensis, 8 parts of Herba Taxilli, 10 parts of Rhizoma Zingiberis Preparatum, 8 parts of Radix Dipsaci, 8 parts of Fructus Schisandrae Chinensis, 9 parts of Fructus Forsythiae, 10 parts of Fructus Psoraleae, 9 parts of Cortex Lycii, 7 parts of Flos Chrysanthemi, bar 25 parts of halberd, 15 parts of Radix Saposhnikoviae, 15 parts of Bulbus Fritillariae Thunbergii, 10 parts of Herba Asari, 8 parts of Bombyx Batryticatus, 9 parts of Ramulus Mori, 25 parts of Oletum Trogopterori, 25 parts of Radix Bupleuri, Cortex Phellodendri 8 6 parts of part, 9 parts of Radix Platycodoniss, 10 parts of Fructus Aurantii Immaturus, 10 parts of Semen Plantaginiss and Fructus Forsythiae.
The preparation method of described Ctenopharyngodon idellus Chinese medicine feed additive, comprises the following steps:
(1) Rhizoma Drynariae, Radix Angelicae Sinensis, Herba Taxilli, Rhizoma Zingiberis Preparatum, Radix Dipsaci, Fructus Schisandrae Chinensis, Fructus Forsythiae, the benefit in formula is weighed by weight Bone fat, Cortex Lycii, Flos Chrysanthemi, Morinda offcinalis How, Radix Saposhnikoviae, Bulbus Fritillariae Thunbergii, Herba Asari, Bombyx Batryticatus, Ramulus Mori, Oletum Trogopterori, Radix Bupleuri, Cortex Phellodendri, Radix Platycodoniss, Fructus Aurantii Immaturus, car Front son, Fructus Forsythiae, clean and dry, and in addition to Herba Asari, Morinda offcinalis How, after being pulverized and mixed by pulverizer, add the tap water of its 3 times of weight, First boil again slow fire by intense fire and endure 0.5h, first filtrate is obtained after filtration, toward medicinal residues the tap water of 2 times of its weight is added, boil by intense fire Afterwards slow fire endures 0.5h and adds that Herba Asari, Morinda offcinalis How are continuous to endure 10min, pours out second filtrate, to merge and be concentrated to give Chinese medicine after filtrate twice Liquid study;
(2) dehydrated alcohol of 3 times of its volume, stirring and dissolving is added to dilute in the herb liquid study obtained by step (1), Flow back 2.5h at a temperature of 85 DEG C, obtains refined herb liquid;
(3) the refined herb liquid obtained by step (2) is taken, after decompression recycling ethanol, by herb liquid low-temperature evaporation, latex shape is obtained Middle medicinal substances, glue milky substance are put in drying machine and are dried, and inlet temperature is 130 DEG C, and leaving air temp is 75 DEG C, obtains powder Material, by powdered rubber 120 mesh are crushed to, and obtain Chinese medicinal powder;
(4) Chinese medicinal powder obtained by step (3) is admixed the Semen Maydis powder of 2 times of its quality, is mixed, carry out expanded, temperature control At 85 DEG C, expanded time 1min, subsequent pelletize, cooling treats that particulate component is cooled to 20 DEG C to system, and packaging obtains final product the feeding of Ctenopharyngodon idellus Chinese medicine Feed additives.
Embodiment 2
A kind of Ctenopharyngodon idellus Chinese medicine feed additive, by being made up of following components by weight:30 parts of Rhizoma Drynariae, 30 parts of Radix Angelicae Sinensis, 20 parts of Herba Taxilli, 20 parts of Rhizoma Zingiberis Preparatum, 16 parts of Radix Dipsaci, 20 parts of Fructus Schisandrae Chinensis, 18 parts of Fructus Forsythiae, 30 parts of Fructus Psoraleae, 20 parts of Cortex Lycii, Flos Chrysanthemi 21 Part, 35 parts of Morinda offcinalis How, 30 parts of Radix Saposhnikoviae, 30 parts of Bulbus Fritillariae Thunbergii, 19 parts of Herba Asari, 15 parts of Bombyx Batryticatus, 20 parts of Ramulus Mori, 35 parts of Oletum Trogopterori, 35 parts of Radix Bupleuri, 15 parts of 15 parts of Cortex Phellodendri, 16 parts of Radix Platycodoniss, 25 parts of Fructus Aurantii Immaturus, 20 parts of Semen Plantaginiss and Fructus Forsythiae.
The preparation method of described Ctenopharyngodon idellus Chinese medicine feed additive, comprises the following steps:
(1) Rhizoma Drynariae, Radix Angelicae Sinensis, Herba Taxilli, Rhizoma Zingiberis Preparatum, Radix Dipsaci, Fructus Schisandrae Chinensis, Fructus Forsythiae, the benefit in formula is weighed by weight Bone fat, Cortex Lycii, Flos Chrysanthemi, Morinda offcinalis How, Radix Saposhnikoviae, Bulbus Fritillariae Thunbergii, Herba Asari, Bombyx Batryticatus, Ramulus Mori, Oletum Trogopterori, Radix Bupleuri, Cortex Phellodendri, Radix Platycodoniss, Fructus Aurantii Immaturus, car Front son, Fructus Forsythiae, clean and dry, and in addition to Herba Asari, Morinda offcinalis How, after being pulverized and mixed by pulverizer, add the tap water of its 4 times of weight, First boil again slow fire by intense fire and endure 1h, first filtrate is obtained after filtration, the tap water of 3 times of its weight is added toward medicinal residues, after boiling by intense fire Slow fire endures 0.5h and adds that Herba Asari, Morinda offcinalis How are continuous to endure 15min, pours out second filtrate, to merge and be concentrated to give herb liquid after filtrate twice Study;
(2) dehydrated alcohol of 5 times of its volume, stirring and dissolving is added to dilute in the herb liquid study obtained by step (1), Flow back 3.5h at a temperature of 95 DEG C, obtains refined herb liquid;
(3) the refined herb liquid obtained by step (2) is taken, after decompression recycling ethanol, by herb liquid low-temperature evaporation, latex shape is obtained Middle medicinal substances, glue milky substance are put in drying machine and are dried, and inlet temperature is 150 DEG C, and leaving air temp is 90 DEG C, obtains powder Material, by powdered rubber 120 mesh are crushed to, and obtain Chinese medicinal powder;
(4) Chinese medicinal powder obtained by step (3) is admixed the Semen Maydis powder of 3 times of its quality, is mixed, carry out expanded, temperature control At 90 DEG C, expanded time 2min, subsequent pelletize, cooling treats that particulate component is cooled to 25 DEG C to system, and packaging obtains final product the feeding of Ctenopharyngodon idellus Chinese medicine Feed additives.
Embodiment 3
A kind of Ctenopharyngodon idellus Chinese medicine feed additive, by being made up of following components by weight:25 parts of Rhizoma Drynariae, 20 parts of Radix Angelicae Sinensis, 12 parts of Herba Taxilli, 15 parts of Rhizoma Zingiberis Preparatum, 12 parts of Radix Dipsaci, 12 parts of Fructus Schisandrae Chinensis, 15 parts of Fructus Forsythiae, 20 parts of Fructus Psoraleae, 15 parts of Cortex Lycii, Flos Chrysanthemi 14 Part, 30 parts of Morinda offcinalis How, 20 parts of Radix Saposhnikoviae, 25 parts of Bulbus Fritillariae Thunbergii, 15 parts of Herba Asari, 12 parts of Bombyx Batryticatus, 12 parts of Ramulus Mori, 30 parts of Oletum Trogopterori, 30 parts of Radix Bupleuri, 9 parts of 12 parts of Cortex Phellodendri, 13 parts of Radix Platycodoniss, 20 parts of Fructus Aurantii Immaturus, 18 parts of Semen Plantaginiss and Fructus Forsythiae.
The preparation method of described Ctenopharyngodon idellus Chinese medicine feed additive, comprises the following steps:
(1) Rhizoma Drynariae, Radix Angelicae Sinensis, Herba Taxilli, Rhizoma Zingiberis Preparatum, Radix Dipsaci, Fructus Schisandrae Chinensis, Fructus Forsythiae, the benefit in formula is weighed by weight Bone fat, Cortex Lycii, Flos Chrysanthemi, Morinda offcinalis How, Radix Saposhnikoviae, Bulbus Fritillariae Thunbergii, Herba Asari, Bombyx Batryticatus, Ramulus Mori, Oletum Trogopterori, Radix Bupleuri, Cortex Phellodendri, Radix Platycodoniss, Fructus Aurantii Immaturus, car Front son, Fructus Forsythiae, clean and dry, and in addition to Herba Asari, Morinda offcinalis How, after being pulverized and mixed by pulverizer, add its 3.5 times of weight originally Water, first boils again slow fire by intense fire and endures 0.8h, and first filtrate is obtained after filtration, and toward medicinal residues the tap water of 2.5 times of its weight, intense fire are added Boil rear slow fire and endure 0.5h and add that Herba Asari, Morinda offcinalis How are continuous to endure 12min, pour out second filtrate, merge and be concentrated to give after filtrate twice Herb liquid study;
(2) dehydrated alcohol of 4 times of its volume, stirring and dissolving is added to dilute in the herb liquid study obtained by step (1), Flow back 3.0h at a temperature of 90 DEG C, obtains refined herb liquid;
(3) the refined herb liquid obtained by step (2) is taken, after decompression recycling ethanol, by herb liquid low-temperature evaporation, latex shape is obtained Middle medicinal substances, glue milky substance are put in drying machine and are dried, and inlet temperature is 140 DEG C, and leaving air temp is 80 DEG C, obtains powder Material, by powdered rubber 120 mesh are crushed to, and obtain Chinese medicinal powder;
(4) Chinese medicinal powder obtained by step (3) is admixed the Semen Maydis powder of 2.5 times of its quality, is mixed, carry out expanded, temperature At 88 DEG C, expanded time 1-2min, subsequent pelletize, cooling treats that particulate component is cooled to 22 DEG C, packaging, in obtaining final product Ctenopharyngodon idellus for control Medicine feed additive.
Embodiment 4:The Ctenopharyngodon idellus Chinese medicine feed additive of the present invention is tested survival rate and resistances against diseases
Experimental animal:Test Ctenopharyngodon idellus are purchased from Nanning City fish farm, are juvenile fish, body weight 80-86g/ tail, selected test fry Number is 4000 tails, and health is strong, and male and female have.4000 tail Ctenopharyngodon idellus are randomly divided into into four groups:Test group 1-3 and matched group, per group Containing the tail of juvenile fish 1000.
Test feed:Chinese medicine feed additive mixed fodder prepared by test group 1-3 difference feeding embodiment of the present invention 1-3 (will 150g above-described embodiments 1-3 prepare Chinese medicine feed additive, 80g soybean cakes, 350g wheat flours, 35g crab shell powders, 60g shrimps Powder, 10g Sal, 160g Semen Maydis powder, 30g fish oil, 15g glycine betaines, 2g dalcium biphosphates, 2g ferric citrates, 40g compound vitamines B, 30g yeast, 20g threonine, 20g lysines, mix homogeneously);The feedstuff of matched group feeding is with the difference of test group:Make The Chinese medicine feed additive for replacing the present invention to use with bambermycin 20mg/kg.
Feeding method:Date of test is 8 days-October 22 May in 2015.Ctenopharyngodon idellus are cultivated in fish pond, stocking density 1 Bar/20L.Fish pond area 1500m2(long 50m × wide 30m), deep 80cm, with bamboo fence, (water can pass through, and fish can not be by being mark It is accurate) separate and block into 375m24 blockages;Fish pond coolant-temperature gage it is whole it is average 16 DEG C (wherein 24-29 DEG C of summer, average 25.5 DEG C), pH value 6.5 ± 0.2, water quality meets fishery standard.
Feedstuff day feeding volume by fry weight 3-5% it is quantitative it is of fixed place and time feed, 2 times/d is fed, every 10d dosages 1 time.Adjust Look into the survival rate of Ctenopharyngodon idellus.
Fish is put to death, with buffer (pH value 7.5,0.25mol/ sucrose, 0.025mol/L Tris-HCL,
0.1mmol/L EDTA-2Na solution) rinse, then surface moisture is sucked with absorbent paper, and remove bone parts, stay The tissue homogenates such as heart, liver and gonad.Tissue 0.30g is accurately weighed, 9 times of buffer ice bath homogenate, 3000r/min is added Low-temperature and high-speed centrifuge 10-15min, takes supernatant 2mL and determines lysozyme and superoxide dismutase vigor.
Test data represents that two sample averages compare to be checked with t, and two samples constitute ratio using means standard deviation (x ± s) With chi-square criterion, P<0.05 has significance for difference.
As a result:Impact of the Chinese medicine feed additive to Ctenopharyngodon idellus survival rate, is shown in Table 1;Chinese medicine feed additive to lysozyme and The impact of superoxide dismutase vigor, the results are shown in Table 2.
Impact of the Ctenopharyngodon idellus Chinese medicine feed additive of the present invention of table 1 to Ctenopharyngodon idellus survival rate
Group Survival number (tail) Death toll (tail) Survival rate (%)
Test group 1 989 11 98.9
Test group 2 985 15 98.5
Test group 3 980 20 98.0
Matched group 836 164 83.6
As it can be seen from table 1 the Chinese medicine feed additive feeding Ctenopharyngodon idellus prepared using the present invention, its ill probability drop Low, survival rate is significantly higher than matched group.
The Ctenopharyngodon idellus Chinese medicine feed additive of the present invention of table 2 is to lysozyme, superoxide dismutase, catalase, alkalescence The impact of Phosphatase Activity
From table 2 it can be seen that using the Ctenopharyngodon idellus of Chinese medicine feed additive feeding of the present invention relative to matched group, internal bacteriolyze Enzyme, superoxide dismutase, catalase, alkaline phosphatase levels are significantly improved, so as to prove the Chinese medicine fodder of the present invention Additive has preferably defence disease, antiviral and antibacterial ability, can significantly improve the immune function of Ctenopharyngodon idellus.
It is aforementioned to the present invention specific illustrative embodiment description be in order to illustrate and illustration purpose.These descriptions It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can be much changed And change.The purpose of selecting and describing the exemplary embodiment is that explaining that the certain principles and its reality of the present invention should With so that those skilled in the art can realize and using the present invention a variety of exemplaries and A variety of selections and change.The scope of the present invention is intended to be limited by claims and its equivalents.

Claims (3)

1. a kind of Ctenopharyngodon idellus Chinese medicine feed additive, it is characterised in that by being made up of following components by weight:Rhizoma Drynariae 20-30 Part, Radix Angelicae Sinensis 10-30 parts, Herba Taxilli 8-20 parts, Rhizoma Zingiberis Preparatum 10-20 parts, Radix Dipsaci 8-16 parts, Fructus Schisandrae Chinensis 8-20 parts, Fructus Forsythiae 9-18 parts, benefit Bone fat 10-30 parts, Cortex Lycii 9-20 parts, Flos Chrysanthemi 7-21 parts, Morinda offcinalis How 25-35 part, Radix Saposhnikoviae 15-30 parts, Bulbus Fritillariae Thunbergii 15-30 part, Herba Asari 10-19 parts, Bombyx Batryticatus 8-15 parts, Ramulus Mori 9-20 parts, Oletum Trogopterori 25-35 parts, Radix Bupleuri 25-35 parts, Cortex Phellodendri 8-15 parts, Radix Platycodoniss 9-16 Part, Fructus Aurantii Immaturus 10-25 parts, Semen Plantaginiss 10-20 parts and Fructus Forsythiae 6-15 parts.
2. Ctenopharyngodon idellus Chinese medicine feed additive according to claim 1, it is characterised in that by by weight by following components system Into:25 parts of Rhizoma Drynariae, 20 parts of Radix Angelicae Sinensis, 12 parts of Herba Taxilli, 15 parts of Rhizoma Zingiberis Preparatum, 12 parts of Radix Dipsaci, 14 parts of Fructus Schisandrae Chinensis, 15 parts of Fructus Forsythiae, Psoralen 25 parts of fat, 15 parts of Cortex Lycii, 12 parts of Flos Chrysanthemi, 30 parts of Morinda offcinalis How, 20 parts of Radix Saposhnikoviae, 25 parts of Bulbus Fritillariae Thunbergii, 15 parts of Herba Asari, 11 parts of Bombyx Batryticatus, Ramulus Mori 10 parts of 15 parts, 30 parts of Oletum Trogopterori, 30 parts of Radix Bupleuri, 12 parts of Cortex Phellodendri, 12 parts of Radix Platycodoniss, 20 parts of Fructus Aurantii Immaturus, 15 parts of Semen Plantaginiss and Fructus Forsythiae.
3. the preparation method of Ctenopharyngodon idellus Chinese medicine feed additive according to claim 1 and 2, it is characterised in that including following Step:
(1) weigh by weight Rhizoma Drynariae in formula, Radix Angelicae Sinensis, Herba Taxilli, Rhizoma Zingiberis Preparatum, Radix Dipsaci, Fructus Schisandrae Chinensis, Fructus Forsythiae, Fructus Psoraleae, Cortex Lycii, Flos Chrysanthemi, Morinda offcinalis How, Radix Saposhnikoviae, Bulbus Fritillariae Thunbergii, Herba Asari, Bombyx Batryticatus, Ramulus Mori, Oletum Trogopterori, Radix Bupleuri, Cortex Phellodendri, Radix Platycodoniss, Fructus Aurantii Immaturus, Semen Plantaginiss, Fructus Forsythiae, cleans and dries, and in addition to Herba Asari, Morinda offcinalis How, after being pulverized and mixed by pulverizer, adds the tap water of its 3-4 times of weight, first military Fire boils slow fire again and endures 0.5-1h, and first filtrate is obtained after filtration, and toward medicinal residues the tap water of its weight 2-3 times is added, and boils by intense fire Afterwards slow fire endures 0.5h and adds that Herba Asari, Morinda offcinalis How are continuous to endure 10-15min, pours out second filtrate, merges in being concentrated to give after filtrate twice Medicinal liquid study;
(2) dehydrated alcohol of its volume 3-5 times, stirring and dissolving is added to dilute in the herb liquid study obtained by step (1), Flow back 2.5-3.5h at a temperature of 85-95 DEG C, obtains refined herb liquid;
(3) the refined herb liquid obtained by step (2) is taken, after decompression recycling ethanol, by herb liquid low-temperature evaporation, latex shape Chinese medicine is obtained Material, glue milky substance is put in drying machine and is dried, and inlet temperature is 130-150 DEG C, and leaving air temp is 75-90 DEG C, is obtained Powdered rubber, by powdered rubber 120 mesh are crushed to, and obtain Chinese medicinal powder;
(4) Chinese medicinal powder obtained by step (3) is admixed the Semen Maydis powder of its quality 2-3 times, is mixed, carry out expanded, temperature control At 85-90 DEG C, expanded time 1-2min, subsequent pelletize, cooling treats that particulate component is cooled to 20-25 DEG C, and packaging obtains final product Ctenopharyngodon idellus Chinese medicine feed additive.
CN201610918111.XA 2016-10-20 2016-10-20 Grass carp Chinese medicinal feed additive and preparation method thereof Pending CN106578657A (en)

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CN107334001A (en) * 2017-07-20 2017-11-10 通化师范学院 A kind of Chinese herbal feed additive for preventing golden trout moth fin disease and application thereof
CN110916015A (en) * 2019-11-27 2020-03-27 江苏三仪生物工程有限公司 Additive for improving antioxidant stress capability of grass carp and preparation method thereof
CN112674239A (en) * 2020-12-24 2021-04-20 集美大学 Chinese herbal medicine compound feed additive for fish and preparation method and application thereof
CN114847414A (en) * 2021-02-04 2022-08-05 山西中医药大学 Functional traditional Chinese medicine fish bait for promoting gastrointestinal health of fishes
CN114903128A (en) * 2022-05-11 2022-08-16 湖南文和友乔口小龙虾养殖有限公司 Crayfish compound feed and preparation method thereof

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CN104171725A (en) * 2014-08-15 2014-12-03 徐州福润禽业食品有限公司 Traditional Chinese medicine hen feed capable of improving egg quality and processing method of feed
CN104187171A (en) * 2014-09-15 2014-12-10 山东新希望六和集团有限公司 Functional feed for preventing and treating red skin disease of grass carp and preparation method

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CN103583928A (en) * 2013-12-02 2014-02-19 郝智慧 Feed used for improving immune functions of grass carp
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Publication number Priority date Publication date Assignee Title
CN107334001A (en) * 2017-07-20 2017-11-10 通化师范学院 A kind of Chinese herbal feed additive for preventing golden trout moth fin disease and application thereof
CN110916015A (en) * 2019-11-27 2020-03-27 江苏三仪生物工程有限公司 Additive for improving antioxidant stress capability of grass carp and preparation method thereof
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CN114847414A (en) * 2021-02-04 2022-08-05 山西中医药大学 Functional traditional Chinese medicine fish bait for promoting gastrointestinal health of fishes
CN114903128A (en) * 2022-05-11 2022-08-16 湖南文和友乔口小龙虾养殖有限公司 Crayfish compound feed and preparation method thereof
CN114903128B (en) * 2022-05-11 2024-01-16 湖南文和友乔口小龙虾养殖有限公司 Crayfish compound feed and preparation method thereof

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Application publication date: 20170426