CN106377656A - Traditional Chinese medicine pre-mixing agent for expelling parasites and preparation method thereof - Google Patents
Traditional Chinese medicine pre-mixing agent for expelling parasites and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
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- A61K33/34—Copper; Compounds thereof
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/42—Cucurbitaceae (Cucumber family)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses a traditional Chinese medicine pre-mixing agent for expelling parasites and a preparation method thereof. The traditional Chinese medicine pre-mixing agent is prepared from 6 to 10 traditional Chinese medicines of the following traditional Chinese medicines: fructus toosendan, semen cucurbitae, fructus carotae, fructus mume, ciderage, black false hellebore, garlic, pericarpium granati, herba scutellariae barbatae, flos genkwa, Chinese honeylocust spine, herba artemisiae annuae, cortex meliae, semen pharbitidis, brucea javanica, gelsemium elegans, chalcanthite, radix euphorbiae ebracteolatae, semen aesculi and radix dichroae. On the basis of the adoption of reasonable traditional Chinese medicine components, effective components capable of expelling parasites are extracted from part of the traditional Chinese medicines via a special extraction process; the effect of expelling parasites of livestock and poultry is improved; toxic and side effects are avoided; meanwhile, the meat quality can also be improved; through adoption of special auxiliary materials, the traditional Chinese medicine pre-mixing agent is prepared into granules; the prepared granules have the effects of preventing feed from being wetted and mildewed.
Description
Technical field
The present invention relates to a kind of Chinese medicine pre-mixing agent for anthelmintic and preparation method thereof, belong to field of veterinary.
Background technology
China is Animal husbandry production big country, and pig, cattle, sheep, poultry raising amount occupy the first in the world, and the number of animals raised of cattle is only second to print
Degree.In recent years, fast to propagating, the high strong epidemic disease of mortality rate, especially to the plague of livestock and fowl jeopardizing human health and life security
The monitoring of disease, Study of Prevention Technology and system aspect construction are all stepped up its investment, and achieve noticeable achievement.By contrast, it is in stealthy sense
Dye, equally has the animal protozoacide of harm and the preventing and treating new drug research of parasitic disease to lag far behind to human health and life security
In the demand in market, class particularly for animals treats protozoacide and the medicine of parasitic disease is more a lack of.
It is known that animal protozoacide, parasitic disease are the mass-sending diseases of livestock and poultry breeding industry one big class severe epidemic, and very
Many protozoacide are serious zoonosiss again, such as eperythrozoonosises, toxoplasmosiss, giardiasis, the sixth of the twelve Earthly Branches bass parasitosis, Taylor
Parasitosis, it is hidden in steamed bun parasitosis, Ah rice's the sixth of the twelve Earthly Branches protozoacide, large intestine balantidiasises etc..Protozoacide morbidity is serious, and mortality rate is high, to herding
The economic loss that industry causes is huge, very big with the threat of health to the life of the mankind.
Content of the invention
It is an object of the invention to provide a kind of Chinese medicine pre-mixing agent for anthelmintic and preparation method thereof, using reasonable Chinese medicine
On the basis of prescription, part raw material of Chinese medicine adopts specific extraction process, has extracted the effective ingredient of anthelmintic, has improve poultry
The effect of anthelmintic, will not produce toxic and side effects, can also improve meat simultaneously, and adopts specific adjuvant, the granule made
Dosage form, has the effect preventing feedstuff from making moist, going mouldy.
A kind of Chinese medicine pre-mixing agent for anthelmintic, selects 6 to 10 tastes to make by the Chinese medicine of following weight proportioning：Fructus Toosendan 5～
10 parts, 60～90 parts of Semen Cucurbitae, 10～15 parts of Fructus Carotae, 3～9 parts of Fructus Mume, 30～60 parts of Herba polygoni hydropiperis, 2～5 parts of Rhizoma et radix veratri (Radix Rhizoma Veratri), Bulbus Allii 15
～30 parts, 3～15 parts of Pericarpium Granati, 30～60 parts of Herba Scutellariae Barbatae, 1.5～3 parts of Flos Genkwa, 3～10 parts of Spina Gleditsiae, 5～15 parts of Herba Artemisiae Annuae, bitter
6～10 parts of chinaberry skin, 3～10 parts of Semen Pharbitidiss, 1～3 part of Fructus Bruceae, 10～30 parts of Herba Gelsemii Elegantis, 0.3～1.5 part of Chalcanthitum, the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) 3～6
Part, 3～10 parts of Semen Aesculi, 10～15 parts of Radix Dichroae.
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) Fructus Carotae extract, Herba polygoni hydropiperis extract, Herba Scutellariae Barbatae extract, Herba Gelsemii Elegantis extract, Radix Dichroae extract, Melia azedarach L. are taken
One or more of peel extract, are mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 7-9 times of Chinese medicine gross weight boils 40-50 minute, filter, filtrate
It is concentrated into the extractum of relative density 1.08-1.12, extractum adds ethanol, make alcohol content reach 50～60%, refilter, then filtrate
It is concentrated into the extractum of relative density 1.10-1.14, be dried, obtain final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 20-30 minute, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
15-20:15-20:8-12:4-6:10-15.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mixing 20-30 divides
Clock, pelletizes, obtains final product.
A kind of Chinese medicine pre-mixing agent for anthelmintic, is made up of the Chinese medicine of following weight proportioning：5～10 parts of Fructus Toosendan, Fructus Cucurbitae moschatae
60～90 parts of son, 10～15 parts of Fructus Carotae, 3～9 parts of Fructus Mume, 30～60 parts of Herba polygoni hydropiperis, 2～5 parts of Rhizoma et radix veratri (Radix Rhizoma Veratri), 15～30 parts of Bulbus Allii, stone
3～15 parts of pomegranate skin, 30～60 parts of Herba Scutellariae Barbatae.
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) take Fructus Carotae extract, Herba polygoni hydropiperis extract, Herba Scutellariae Barbatae extract, be mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 7-9 times of Chinese medicine gross weight boils 40-50 minute, filter, filtrate
It is concentrated into the extractum of relative density 1.08-1.12, extractum adds ethanol, make alcohol content reach 50～60%, refilter, then filtrate
It is concentrated into the extractum of relative density 1.10-1.14, be dried, obtain final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 20-30 minute, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
15-20:15-20:8-12:4-6:10-15.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mixing 20-30 divides
Clock, pelletizes, obtains final product.
A kind of Chinese medicine pre-mixing agent for anthelmintic, is made up of the Chinese medicine of following weight proportioning：1.5～3 parts of Flos Genkwa, Spina Gleditsiae
3～10 parts, 5～15 parts of Herba Artemisiae Annuae, 6～10 parts of Cortex Meliae, 3～10 parts of Semen Pharbitidiss, 1～3 part of Fructus Bruceae, 10～30 parts of Herba Gelsemii Elegantis, gallbladder
0.3～1.5 part of vitriol, 3～6 parts of the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae), 3～10 parts of Semen Aesculi, 10～15 parts of Radix Dichroae.
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) hook taking kiss extract, Radix Dichroae extract, Cortex Meliae extract, are mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 7-9 times of Chinese medicine gross weight boils 40-50 minute, filter, filtrate
It is concentrated into the extractum of relative density 1.08-1.12, extractum adds ethanol, make alcohol content reach 50～60%, refilter, then filtrate
It is concentrated into the extractum of relative density 1.10-1.14, be dried, obtain final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 20-30 minute, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
15-20:15-20:8-12:4-6:10-15.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mixing 20-30 divides
Clock, pelletizes, obtains final product.
The preextraction of Fructus Carotae：By the Fructus Carotae boiling water heat-insulation soaking 10-14h of 9-11 times of quality, filter, obtain filtrate
a；It is 1.04-1.06 that filtrate a is evaporated to relative density, obtains extractum；90% ethanol of extractum plus 2-3 times of volume,
Stir, filter to get filtrate b；The n-butanol extraction of filtrate b equal volume, separate and obtain n-butanol layer, recovered under reduced pressure
N-butyl alcohol, is dried, obtains final product Fructus Carotae extract.
The preextraction of Herba polygoni hydropiperis：Herba polygoni hydropiperis are pulverized, boils 20-30 minute with 7-9 times of decocting, filter, obtain medicinal residues；Medicinal residues
With dehydrated alcohol-ether mixed solution soaking at room temperature 4-6h of 6-8 times of quality, it is centrifuged, separate and obtain supernatant；Supernatant plus
The activated carbon of 2% mass, filters, obtains filtrate 1.；Filtrate 1. add 95% ethanol to be made alcohol content to be 70%, filter, obtain filtrate 2.,
2. filtrate be evaporated to no alcohol taste, be dried, obtain final product Herba polygoni hydropiperis extract.
Wherein in dehydrated alcohol-ether mixed solution, the volume of dehydrated alcohol and ether is 2:1.
The preextraction of Herba Scutellariae Barbatae：Herba Scutellariae Barbatae is pulverized, plus 70-80 DEG C of 75% ethanol of 14-16 times of quality is heated to reflux
180-220min, filters, obtains filtrate；Upper for filtrate HZ002 ion exchange absorption resin column, upper column flow rate is 1.1-1.3BV/h,
Again use 3BV 30% methanol -70% water, 20% methanol -80% water of 2BV, 10% methanol -90% water of 1BV, successively gradient wash
De-, elution flow rate is 1.1-1.3BV/h, collect and combine 30% methanol -70% water, 20% methanol -80% water, 10% methanol -
90% water elution, the 1/3 of eluent decompression and solvent recovery to original volume, is spray-dried, as Herba Scutellariae Barbatae extract.
Wherein, the volume ratio of filtrate and HZ002 ion exchange absorption resin is 2:1.
The preextraction of Herba Gelsemii Elegantis：Herba Gelsemii Elegantis are pulverized, is carried with 40-50 DEG C of the n-hexane-ethyl acetate mixed liquor of 13-15 times of quality
Take 2-3h, filter, obtain filtrate；It is 8-9 that filtrate is adjusted pH value, and centrifugation separates and obtains supernatant；Supernatant is evaporated to
Relative density is the extractum of 1.11-1.13, is dried, obtains Herba Gelsemii Elegantis extract.
Wherein in n-hexane-ethyl acetate mixed liquor, the volume ratio of normal hexane and ethyl acetate is 1:3.
The preextraction of Radix Dichroae：Radix Dichroae is pulverized, plus the acetone supersound extraction 30-40 minute of 3-5 times of quality, filter, obtain filter
Slag；60-70 DEG C of heating and refluxing extraction 120-180min of methanol of filtering residue plus 10-12 times of quality, filter, obtain filtrate；Filtrate
Upper AB-8 macroporous adsorptive resins, upper column flow rate is 1.5-1.7BV/h, then 20% ethanol -80% acetone with 5BV, 3BV
40% ethanol -60% acetone, 80% ethanol -20% acetone of 1BV, gradient elution successively, elution flow rate is 1.5-1.7BV/h,
Collect and combine 20% ethanol -80% acetone, 40% ethanol -60% acetone, 80% ethanol -20% acetone eluant, eluent
Recovered under reduced pressure, to no alcohol taste, is spray-dried, as Radix Dichroae extract.
Wherein, the volume ratio of filtrate and AB-8 macroporous adsorbent resin is 2.5:1.
The preextraction of Cortex Meliae：Cortex Meliae is pulverized, plus the benzene of 10-12 times of quality-acetone and ethyl acetate mixed liquor 150W
Microwave extraction 30 minutes, filters, obtains filtrate；Filtrate reduced in volume recycling design, it is dried, obtains final product Cortex Meliae extract.
Wherein, in benzene-acetone and ethyl acetate mixed liquor, benzene, acetone, the volume ratio of ethyl acetate are 2:3:4.
The using method of the present invention：1.0-1.5 kilogram of finished product, direct feeding is mixed thoroughly in feedstuff per ton.
The present invention, from Chinese medicine various in style, from medical material action principle, action and efficacy angularly, tests through multi-party,
Determine reasonable formula and the consumption of the present invention eventually, and according to processing of crude drugs characteristic, preextraction is carried out to part material, extracts
Effective ingredient to poultry anthelmintic, improves bioavailability, thus improve the curative effect of product anthelmintic, adds in adjuvant simultaneously
Palmic acid, alpha-linolenic acid, the bead dosage form made, not only can improve meat, the effect also prevent feedstuff from making moist, going mouldy.
Specific embodiment：
Embodiment 1：
A kind of Chinese medicine pre-mixing agent for anthelmintic, is made up of the Chinese medicine of following weight proportioning：5 parts of Fructus Toosendan, Semen Cucurbitae 60
Part, 10 parts of Fructus Carotae, 3 parts of Fructus Mume, 30 parts of Herba polygoni hydropiperis, 2 parts of Rhizoma et radix veratri (Radix Rhizoma Veratri), 15 parts of Bulbus Allii, 3 parts of Pericarpium Granati, 30 parts of Herba Scutellariae Barbatae.
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) take Fructus Carotae extract, Herba polygoni hydropiperis extract, Herba Scutellariae Barbatae extract, be mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 7 times of Chinese medicine gross weights boils 40 minutes, filter, filtrate is concentrated into
The extractum of relative density 1.08, extractum adds ethanol, makes alcohol content reach 50%, refilters, then filtrate is concentrated into relative density
1.10 extractum, is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 20-30 minute, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
15:15:8:4:10.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 20 minutes,
Pelletize, obtain final product.
The preextraction of Fructus Carotae：By the Fructus Carotae boiling water heat-insulation soaking 10h of 9 times of quality, filter, obtain filtrate a；Filter
It is 1.04 that liquid a is evaporated to relative density, obtains extractum；90% ethanol of extractum plus 2 times of volumes, stir, filter
Obtain filtrate b；The n-butanol extraction of filtrate b equal volume, separate and obtain n-butanol layer, recovered under reduced pressure n-butyl alcohol, it is dried, that is,
Obtain Fructus Carotae extract.
The preextraction of Herba polygoni hydropiperis：Herba polygoni hydropiperis are pulverized, is boiled 20 minutes with 7 times of decocting, filter, obtain medicinal residues；Medicinal residues with 6 times
The dehydrated alcohol of quality-ether mixed solution soaking at room temperature 4h, centrifugation, separate and obtain supernatant；Supernatant plus 2% mass
Activated carbon, filters, obtains filtrate 1.；Filtrate 1. add 95% ethanol to be made alcohol content to be 70%, filters, obtain filtrate 2., filtrate 2.
It is evaporated to no alcohol taste, be dried, obtain final product Herba polygoni hydropiperis extract.
Wherein in dehydrated alcohol-ether mixed solution, the volume of dehydrated alcohol and ether is 2:1.
The preextraction of Herba Scutellariae Barbatae：Herba Scutellariae Barbatae is pulverized, plus 70 DEG C of 75% ethanol of 14 times of quality is heated to reflux 180min, mistake
Filter, obtains filtrate；Upper for filtrate HZ002 ion exchange absorption resin column, upper column flow rate is 1.1BV/h, then 30% first with 3BV
Alcohol -70% water, 20% methanol -80% water of 2BV, 10% methanol -90% water of 1BV, gradient elution successively, elution flow rate is
1.1BV/h, collects and combines 30% methanol -70% water, 20% methanol -80% water, 10% methanol -90% water elution, eluting
Liquid decompression and solvent recovery, to the 1/3 of original volume, is spray-dried, as Herba Scutellariae Barbatae extract.
Wherein, the volume ratio of filtrate and HZ002 ion exchange absorption resin is 2:1.
Embodiment 2：
A kind of Chinese medicine pre-mixing agent for anthelmintic, is made up of the Chinese medicine of following weight proportioning：8 parts of Fructus Toosendan, Semen Cucurbitae 75
Part, 12 parts of Fructus Carotae, 6 parts of Fructus Mume, 45 parts of Herba polygoni hydropiperis, 3 parts of Rhizoma et radix veratri (Radix Rhizoma Veratri), 20 parts of Bulbus Allii, 8 parts of Pericarpium Granati, 45 parts of Herba Scutellariae Barbatae.
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) take Fructus Carotae extract, Herba polygoni hydropiperis extract, Herba Scutellariae Barbatae extract, be mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 8 times of Chinese medicine gross weights boils 45 minutes, filter, filtrate is concentrated into
The extractum of relative density 1.10, extractum adds ethanol, makes alcohol content reach 55%, refilters, then filtrate is concentrated into relative density
1.12 extractum, is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 25 minutes, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
18:18:10:5:12.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 25 minutes,
Pelletize, obtain final product.
The preextraction of Fructus Carotae：By the Fructus Carotae boiling water heat-insulation soaking 12h of 10 times of quality, filter, obtain filtrate a；?
It is 1.05 that filtrate a is evaporated to relative density, obtains extractum；90% ethanol of extractum plus 2.5 times of volumes, stir,
Filter to get filtrate b；The n-butanol extraction of filtrate b equal volume, separate and obtain n-butanol layer, recovered under reduced pressure n-butyl alcohol, do
Dry, obtain final product Fructus Carotae extract.
The preextraction of Herba polygoni hydropiperis：Herba polygoni hydropiperis are pulverized, is boiled 25 minutes with 10 times of decocting, filter, obtain medicinal residues；Medicinal residues are used 7
Dehydrated alcohol-ether mixed solution soaking at room temperature 5h of times quality, centrifugation, separate and obtain supernatant；Supernatant plus 2% mass
Activated carbon, filter, obtain filtrate 1.；It is 70% that filtrate plus 95% ethanol are made alcohol content, filters, obtains filtrate 2., filtrate 2.
It is evaporated to no alcohol taste, be dried, obtain final product Herba polygoni hydropiperis extract.
Wherein in dehydrated alcohol-ether mixed solution, the volume of dehydrated alcohol and ether is 2:1.
The preextraction of Herba Scutellariae Barbatae：Herba Scutellariae Barbatae is pulverized, plus 75 DEG C of 75% ethanol of 15 times of quality is heated to reflux 200min, mistake
Filter, obtains filtrate；Upper for filtrate HZ002 ion exchange absorption resin column, upper column flow rate is 1.2BV/h, then 30% first with 3BV
Alcohol -70% water, 20% methanol -80% water of 2BV, 10% methanol -90% water of 1BV, gradient elution successively, elution flow rate is
1.2BV/h, collects and combines 30% methanol -70% water, 20% methanol -80% water, 10% methanol -90% water elution, eluting
Liquid decompression and solvent recovery, to the 1/3 of original volume, is spray-dried, as Herba Scutellariae Barbatae extract.
Wherein, the volume ratio of filtrate and HZ002 ion exchange absorption resin is 2:1.
Embodiment 3：
A kind of Chinese medicine pre-mixing agent for anthelmintic, is made up of the Chinese medicine of following weight proportioning：10 parts of Fructus Toosendan, Semen Cucurbitae 90
Part, 15 parts of Fructus Carotae, 9 parts of Fructus Mume, 60 parts of Herba polygoni hydropiperis, 5 parts of Rhizoma et radix veratri (Radix Rhizoma Veratri), 30 parts of Bulbus Allii, 15 parts of Pericarpium Granati, 60 parts of Herba Scutellariae Barbatae.
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) take Fructus Carotae extract, Herba polygoni hydropiperis extract, Herba Scutellariae Barbatae extract, be mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 9 times of Chinese medicine gross weights boils 50 minutes, filter, filtrate is concentrated into
The extractum of relative density 1.12, extractum adds ethanol, makes alcohol content reach 60%, refilters, then filtrate is concentrated into relative density
1.14 extractum, is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 30 minutes, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
20:20:12:6:15.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 30 minutes,
Pelletize, obtain final product.
The preextraction of Fructus Carotae：By the Fructus Carotae boiling water heat-insulation soaking 14h of 11 times of quality, filter, obtain filtrate a；?
It is 1.06 that filtrate a is evaporated to relative density, obtains extractum；90% ethanol of extractum plus 3 times of volumes, stir, mistake
Filter to obtain filtrate b；The n-butanol extraction of filtrate b equal volume, separate and obtain n-butanol layer, recovered under reduced pressure n-butyl alcohol, it is dried,
Obtain final product Fructus Carotae extract.
The preextraction of Herba polygoni hydropiperis：Herba polygoni hydropiperis are pulverized, is boiled 30 minutes with 9 times of decocting, filter, obtain medicinal residues；Medicinal residues with 8 times
The dehydrated alcohol of quality-ether mixed solution soaking at room temperature 6h, centrifugation, separate and obtain supernatant；Supernatant plus 2% mass
Activated carbon, filters, obtains filtrate 1.；It is 70% that filtrate plus 95% ethanol are made alcohol content, filters, obtains filtrate 2., 2. filtrate subtracted
Pressure is concentrated into no alcohol taste, is dried, obtains final product Herba polygoni hydropiperis extract.
Wherein in dehydrated alcohol-ether mixed solution, the volume of dehydrated alcohol and ether is 2:1.
The preextraction of Herba Scutellariae Barbatae：Herba Scutellariae Barbatae is pulverized, plus 80 DEG C of 75% ethanol of 16 times of quality is heated to reflux 220min, mistake
Filter, obtains filtrate；Upper for filtrate HZ002 ion exchange absorption resin column, upper column flow rate is 1.3BV/h, then 30% first with 3BV
Alcohol -70% water, 20% methanol -80% water of 2BV, 10% methanol -90% water of 1BV, gradient elution successively, elution flow rate is
1.3BV/h, collects and combines 30% methanol -70% water, 20% methanol -80% water, 10% methanol -90% water elution, eluting
Liquid decompression and solvent recovery, to the 1/3 of original volume, is spray-dried, as Herba Scutellariae Barbatae extract.
Wherein, the volume ratio of filtrate and HZ002 ion exchange absorption resin is 2:1.
Embodiment 4：
A kind of Chinese medicine pre-mixing agent for anthelmintic, is made up of the Chinese medicine of following weight proportioning：1.5 parts of Flos Genkwa, Spina Gleditsiae 3
Part, 5 parts of Herba Artemisiae Annuae, 6 parts of Cortex Meliae, 3 parts of Semen Pharbitidiss, 1 part of Fructus Bruceae, 10 parts of Herba Gelsemii Elegantis, 0.3 part of Chalcanthitum, 3 parts of the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae), Semen Aesculi 3
Part, 10 parts of Radix Dichroae.
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) hook taking kiss extract, Radix Dichroae extract, Cortex Meliae extract, are mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 7 times of Chinese medicine gross weights boils 40 minutes, filter, filtrate is concentrated into
The extractum of relative density 1.08, extractum adds ethanol, makes alcohol content reach 50%, refilters, then filtrate is concentrated into relative density
1.10 extractum, is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 20 minutes, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
15:15:8:4:10.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 20 minutes,
Pelletize, obtain final product.
The preextraction of Herba Gelsemii Elegantis：Herba Gelsemii Elegantis are pulverized, extract 2h with 40 DEG C of the n-hexane-ethyl acetate mixed liquor of 13 times of quality,
Filter, obtain filtrate；It is 8-9 that filtrate is adjusted pH value, and centrifugation separates and obtains supernatant；Supernatant is evaporated to relatively close
Spend the extractum for 1.11, be dried, obtain Herba Gelsemii Elegantis extract.
Wherein in n-hexane-ethyl acetate mixed liquor, the volume ratio of normal hexane and ethyl acetate is 1:3.
The preextraction of Radix Dichroae：Radix Dichroae is pulverized, plus the acetone supersound extraction of 3 times of quality 30 minutes, filter, obtain filtering residue；?
Filtering residue adds 60 DEG C of heating and refluxing extraction 120min of methanol of 10 times of quality, filters, obtains filtrate；Upper for filtrate AB-8 macroporous absorption tree
Fat post, upper column flow rate is 1.5BV/h, then 40% ethanol -60% acetone of 20% ethanol -80% acetone with 5BV, 3BV, 1BV
80% ethanol -20% acetone, gradient elution successively, elution flow rate be 1.5BV/h, collect and combine 20% ethanol -80% acetone,
40% ethanol -60% acetone, 80% ethanol -20% acetone eluant, eluent recovered under reduced pressure to no alcohol taste, are spray-dried,
It is Radix Dichroae extract.
Wherein, the volume ratio of filtrate and AB-8 macroporous adsorbent resin is 2.5:1.
The preextraction of Cortex Meliae：Cortex Meliae is pulverized, plus the benzene of 10 times of quality-acetone and ethyl acetate mixed liquor 150W is micro-
Ripple extracts 30 minutes, filters, obtains filtrate；Filtrate reduced in volume recycling design, it is dried, obtains final product Cortex Meliae extract.
Wherein, in benzene-acetone and ethyl acetate mixed liquor, benzene, acetone, the volume ratio of ethyl acetate are 2:3:4.
Embodiment 5：
A kind of Chinese medicine pre-mixing agent for anthelmintic, is made up of the Chinese medicine of following weight proportioning：2 parts of Flos Genkwa, 6 parts of Spina Gleditsiae,
10 parts of Herba Artemisiae Annuae, 8 parts of Cortex Meliae, 6 parts of Semen Pharbitidiss, 2 parts of Fructus Bruceae, 20 parts of Herba Gelsemii Elegantis, 1.0 parts of Chalcanthitum, 5 parts of the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae), 6 parts of Semen Aesculi,
12 parts of Radix Dichroae.
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) hook taking kiss extract, Radix Dichroae extract, Cortex Meliae extract, are mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 8 times of Chinese medicine gross weights boils 45 minutes, filter, filtrate is concentrated into
The extractum of relative density 1.10, extractum adds ethanol, makes alcohol content reach 55%, refilters, then filtrate is concentrated into relative density
1.12 extractum, is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 25 minutes, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
18:18:10:5:12.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 25 minutes,
Pelletize, obtain final product.
The preextraction of Herba Gelsemii Elegantis：Herba Gelsemii Elegantis are pulverized, with 45 DEG C of extractions of n-hexane-ethyl acetate mixed liquor of 14 times of quality
2.5h, filters, obtains filtrate；It is 8-9 that filtrate is adjusted pH value, and centrifugation separates and obtains supernatant；Supernatant is evaporated to phase
It is 1.12 extractum to density, be dried, obtain Herba Gelsemii Elegantis extract.
Wherein in n-hexane-ethyl acetate mixed liquor, the volume ratio of normal hexane and ethyl acetate is 1:3.
The preextraction of Radix Dichroae：Radix Dichroae is pulverized, plus the acetone supersound extraction of 4 times of quality 35 minutes, filter, obtain filtering residue；?
Filtering residue adds 65 DEG C of heating and refluxing extraction 150min of methanol of 11 times of quality, filters, obtains filtrate；Upper for filtrate AB-8 macroporous absorption tree
Fat post, upper column flow rate is 1.6BV/h, then 40% ethanol -60% acetone of 20% ethanol -80% acetone with 5BV, 3BV, 1BV
80% ethanol -20% acetone, gradient elution successively, elution flow rate be 1.6BV/h, collect and combine 20% ethanol -80% acetone,
40% ethanol -60% acetone, 80% ethanol -20% acetone eluant, eluent recovered under reduced pressure to no alcohol taste, are spray-dried,
It is Radix Dichroae extract.
Wherein, the volume ratio of filtrate and AB-8 macroporous adsorbent resin is 2.5:1.
The preextraction of Cortex Meliae：Cortex Meliae is pulverized, plus the benzene of 11 times of quality-acetone and ethyl acetate mixed liquor 150W is micro-
Ripple extracts 30 minutes, filters, obtains filtrate；Filtrate reduced in volume recycling design, it is dried, obtains final product Cortex Meliae extract.
Wherein, in benzene-acetone and ethyl acetate mixed liquor, benzene, acetone, the volume ratio of ethyl acetate are 2:3:4.
Embodiment 6：
A kind of Chinese medicine pre-mixing agent for anthelmintic, is made up of the Chinese medicine of following weight proportioning：3 parts of Flos Genkwa, 10 parts of Spina Gleditsiae,
15 parts of Herba Artemisiae Annuae, 10 parts of Cortex Meliae, 10 parts of Semen Pharbitidiss, 3 parts of Fructus Bruceae, 30 parts of Herba Gelsemii Elegantis, 1.5 parts of Chalcanthitum, 6 parts of the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae), Semen Aesculi 10
Part, 15 parts of Radix Dichroae.
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) hook taking kiss extract, Radix Dichroae extract, Cortex Meliae extract, are mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 9 times of Chinese medicine gross weights boils 50 minutes, filter, filtrate is concentrated into
The extractum of relative density 1.12, extractum adds ethanol, makes alcohol content reach 60%, refilters, then filtrate is concentrated into relative density
1.14 extractum, is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 30 minutes, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
20:20:12:6:15.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 30 minutes,
Pelletize, obtain final product.
The preextraction of Herba Gelsemii Elegantis：Herba Gelsemii Elegantis are pulverized, extract 3h with 50 DEG C of the n-hexane-ethyl acetate mixed liquor of 15 times of quality,
Filter, obtain filtrate；It is 8-9 that filtrate is adjusted pH value, and centrifugation separates and obtains supernatant；Supernatant is evaporated to relatively close
Spend the extractum for 1.13, be dried, obtain Herba Gelsemii Elegantis extract.
Wherein in n-hexane-ethyl acetate mixed liquor, the volume ratio of normal hexane and ethyl acetate is 1:3.
The preextraction of Radix Dichroae：Radix Dichroae is pulverized, plus the acetone supersound extraction of 5 times of quality 40 minutes, filter, obtain filtering residue；?
Filtering residue adds 70 DEG C of heating and refluxing extraction 180min of methanol of 12 times of quality, filters, obtains filtrate；Upper for filtrate AB-8 macroporous absorption tree
Fat post, upper column flow rate is 1.7BV/h, then 40% ethanol -60% acetone of 20% ethanol -80% acetone with 5BV, 3BV, 1BV
80% ethanol -20% acetone, gradient elution successively, elution flow rate be 1.7BV/h, collect and combine 20% ethanol -80% acetone,
40% ethanol -60% acetone, 80% ethanol -20% acetone eluant, eluent recovered under reduced pressure to no alcohol taste, are spray-dried,
It is Radix Dichroae extract.
Wherein, the volume ratio of filtrate and AB-8 macroporous adsorbent resin is 2.5:1.
The preextraction of Cortex Meliae：Cortex Meliae is pulverized, plus the benzene of 12 times of quality-acetone and ethyl acetate mixed liquor 150W is micro-
Ripple extracts 30 minutes, filters, obtains filtrate；Filtrate reduced in volume recycling design, it is dried, obtains final product Cortex Meliae extract.
Wherein, in benzene-acetone and ethyl acetate mixed liquor, benzene, acetone, the volume ratio of ethyl acetate are 2:3:4.
Comparative example 1：
A kind of Chinese medicine pre-mixing agent for anthelmintic, is made up of the Chinese medicine of following weight proportioning：15 parts of Fructus Toosendan, Semen Cucurbitae 50
Part, 20 parts of Fructus Carotae, 1 part of Fructus Mume, 70 parts of Herba polygoni hydropiperis, 1 part of Rhizoma et radix veratri (Radix Rhizoma Veratri), 40 parts of Bulbus Allii, 20 parts of Pericarpium Granati, 20 parts of Herba Scutellariae Barbatae.
Remaining is with embodiment 1.
Comparative example 2：
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) take remaining Chinese medicine in formula, plus the decocting of 7 times of Chinese medicine gross weights boils 40 minutes, filter, filtrate is concentrated into
The extractum of relative density 1.08, extractum adds ethanol, makes alcohol content reach 50%, refilters, then filtrate is concentrated into relative density
1.10 extractum, is dried, obtains final product Chinese medicine extract；
(2) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Palmic acid, α-Caulis et Folium Lini
Acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 20 minutes, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:
15:15:8:4:10.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mixing 20-30 divides
Clock, pelletizes, obtains final product.
Remaining is with embodiment 4.
Comparative example 3：
The preextraction of Fructus Carotae：By the Fructus Carotae boiling water heat-insulation soaking 12h of 10 times of quality, filter, obtain filtrate a；?
It is 1.05 that filtrate a is evaporated to relative density, obtains extractum；70% ethanol of extractum plus 2.5 times of volumes, stir,
Filter to get filtrate b；The chloroform extraction of filtrate b equal volume, separate and obtain chloroform layer, recovered under reduced pressure chloroform, it is dried, obtain final product
Fructus Carotae extract.
The preextraction of Herba polygoni hydropiperis：Herba polygoni hydropiperis are pulverized, is boiled 25 minutes with 10 times of decocting, filter, obtain medicinal residues；Medicinal residues are used 7
Dehydrated alcohol-acetone mixed solution soaking at room temperature 5h of times quality, centrifugation, separate and obtain supernatant；Supernatant plus 2% mass
Activated carbon, filter, obtain filtrate 1.；It is 70% that filtrate plus 90% ethanol are made alcohol content, filters, obtains filtrate 2., filtrate 2.
It is evaporated to no alcohol taste, be dried, obtain final product Herba polygoni hydropiperis extract.
Wherein in dehydrated alcohol-acetone mixed solution, the volume of dehydrated alcohol and acetone is 2:1.
The preextraction of Herba Scutellariae Barbatae：Herba Scutellariae Barbatae is pulverized, plus 75 DEG C of 75% ethanol of 15 times of quality is heated to reflux 200min, mistake
Filter, obtains filtrate；Upper for filtrate HD-8 ion exchange absorption resin column, upper column flow rate is 1.2BV/h, then with 30% methanol of 3BV-
70% water, 20% methanol -80% water of 2BV, 10% methanol -90% water of 1BV, gradient elution successively, elution flow rate is
1.2BV/h, collects and combines 30% methanol -70% water, 20% methanol -80% water, 10% methanol -90% water elution, eluting
Liquid decompression and solvent recovery, to the 1/3 of original volume, is spray-dried, as Herba Scutellariae Barbatae extract.
Wherein, the volume ratio of filtrate and HD-8 ion exchange absorption resin is 2:1.
Remaining is with embodiment 2.
Comparative example 4：
A kind of Chinese medicine pre-mixing agent for anthelmintic, preparation method is：
(1) hook taking kiss extract, Radix Dichroae extract, Cortex Meliae extract, are mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 7 times of Chinese medicine gross weights boils 40 minutes, filter, filtrate is concentrated into
The extractum of relative density 1.08, extractum adds ethanol, makes alcohol content reach 50%, refilters, then filtrate is concentrated into relative density
1.10 extractum, is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, the extract of addition pretreatment, Pregelatinized Starch, paste
Essence, Microcrystalline Cellulose, mix 20 minutes, standby；
Chinese medicine extract with the mass ratio of Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:8:4:10.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 20 minutes,
Pelletize, obtain final product.
Remaining is with embodiment 5.
Animal experiment：
1. the medicine effect evaluation test of antibody endoparasite of the present invention：
1.1 animal subject
8 week old piglets 800, for the test of pig parasitic treatments.
1.2 method
1.2.1 test pig is randomly divided into 10 groups, every group 80, respectively test blank control group, embodiment 1-6 group,
Comparative example 1-3 group.In addition to blank control group, other each groups all add, according to feedstuff per ton, the premix that embodiment and comparative example are obtained
After agent 1kg is normally fed January, pig health status are carried out with situations such as outward appearance, body weight checks and is compared；
1.2.2 experimental treatment：After test pig carries out normal anthelmintic, pig feed intake increases, and pig late growing stage increases weight
Rate is high；Simultaneously after good anthelmintic, pig body surface fur light；
1.2.3 index of assessment of curative effect
(1) no parasitic infection rate and parasitic infection rate：
Healthy (no parasitic infection)：Pig feed intake is normal or increases, body surface fur light；Parasitic infection：Exist
Significantly parasite dye sense symptom, feed intake declines, and body surface fur color and luster is not bright；
(2) average weight gain：According to the body weight of every pig before administration and during off-test, the gain in weight of every pig of calculating, according to
This calculates every group of average weight gain；Body weight increase rate：Calculate by the ratio of each medication group and the average weight gain of blank control group；
1.3 conclusion
The statistical disposition of data carries out the significance test of data with biometrics, is shown in Table 1；
Table 1 present invention is to the treatment of pig parasitic disease and prophylactic tria
Find out from the result of table 1, the therapeutic test effective percentage to pig parasites disease in the body for the embodiments of the invention 1-6
Height, average daily gain is also higher, and effect is significant is better than 1 group of comparative example (formula consumption is different), (raw material of Chinese medicine is not for 2 groups of comparative example
Using pretreating process), 3 groups of comparative example (pretreating process different) it is seen that on the basis of the present invention adopts reasonable Chinese prescription,
Part raw material of Chinese medicine adopts specific handling process, effectively extracts the effective ingredient of preventing and treating parasite, improves piglet daily gain,
Prepared Chinese medicine pre-mixing agent has good anthelminthic effect.
2. clinical therapeutic efficacy of the present invention
The clinical therapeutic efficacy of table 2 present invention
Find out from the result of table 2, embodiments of the invention 1-6 is higher to the therapeutic effect of parasitic disease, is significantly better than
1 group of comparative example (formula consumption is different), 2 groups of comparative example (raw material of Chinese medicine is provided without pretreating process), 3 groups of (pretreatment of comparative example
Technique is different) it is seen that the present invention is using on the basis of reasonable Chinese prescription, part raw material of Chinese medicine adopts specific handling process,
Effectively extract the effective ingredient of preventing and treating parasite, improve piglet daily gain, prepared Chinese medicine pre-mixing agent has good anthelmintic to imitate
Really.
3. the impact to growth fattening pork mass for the present invention
(boar goes the DLY tri-crossbreeding close from 65 age in days parity, average initial weight is (34.68 ± 3.86) kg
Gesture, male and female half and half) 45,5 nest carries out 60d raising experiment, and experiment adopts single-factor randomized block design, is divided into blank group, enforcement
Example 1-6 group, comparative example 1-3 group, every group 5 repetitions, pig 3 (6.25 square metres of colony house area) raised by every nest.Diet basis is
Conventional use of Corn-soybean-cotton rapeseed meal type daily ration in China's Swine Production.
During experiment：1. embodiment 1-6 group, comparative example 1-3 group press embodiment 1-6 group respectively, comparative example 1-3 group is obtained
Chinese medicine pre-mixing agent is added on feedstuff, and feedstuff per ton adds in 1kg pre-mixing agent, blank group without；2. timing feeding 3 times daily,
It is respectively 7：00、14：00、18：00, periodically fed and registered, free water, daily cleaning up excrement once, keeps cleaning in circle；
3. indoor daily Daily temperature range and weather condition are recorded.
During off-test, selective body focuses on the castration boar in the range of (90 ± 3) kg, butchers 4 for every group.Before killing on an empty stomach
24h, normal supply of drinking water, after the lethal hot chicken hair of blood-letting, along midvertebral line and ventrimeson, pig corpse is divided into left and right two parts.
Percentage of water loss adopts oven drying method；The poor by weight method of drip loss preserves 24h at 4 DEG C and is measured；Tenderness, using shearing force method, is used
Saitermodel-235 (method is domestic) tender degree device measures.
The impact (means standard deviation) to growth fattening pork mass for table 3 present invention
Find out from the result of table 3, embodiments of the invention 1-6 significantly reduces drip loss, percentage of water loss and tenderness and cuts
Shear force, improve Carnis Sus domestica is waterpower and tenderness, and effect is significant is better than 1 group of comparative example (formula consumption is different), 2 groups of comparative example
3 groups of (raw material of Chinese medicine is provided without pretreating process), comparative example (pretreating process is different) is it is seen that the present invention adopts reasonable Chinese medicine group
On the basis of side, part raw material of Chinese medicine adopt specific handling process, in adjuvant add Palmic acid, alpha-linolenic acid, make
Granule type, can improve meat effect.
4. the impact that the present invention deposits to TRM
Fresh TMR (being made up of concentrate supplement, ensiling, Leymus chinensis (Trin.) Tzvel. and straw, moisture about 50%)；Weigh same day production
TMR feedstuff, be respectively added to TMR feedstuff, feedstuff per ton according to the Chinese medicine pre-mixing agent that embodiment 1-6 and comparative example 2 and 4 are obtained
Add 1kg pre-mixing agent, be averagely dispensed in 9 woven bags after mixing step by step, every bag of 20kg.Matched group is not added with any material.Treat
After sealing bag mouth, it is deposited in successively in the workshop of feed factory.
The daily situation of going mouldy observing TMR feedstuff from the 2nd day of storage, according to the criterion record result of table 4.Altogether
Observe 12d.Result is as shown in table 5.
Table 4 goes mouldy feature in various degree
Table 5 present invention is to the impact that TRM is deposited
Find out from the result of table 5, the 9th talent's generation point-like is mouldy again for embodiments of the invention 1-6, and anti-mold effect is significantly excellent
In 2 groups of comparative example (raw material of Chinese medicine is provided without pretreating process), 4 groups of comparative example (adjuvant is not added with Palmic acid, alpha-linolenic acid), can
On the basis of seeing that the present invention adopts reasonable Chinese prescription, part raw material of Chinese medicine adopts specific handling process, adds palm fibre in adjuvant
Palmitic acid acid, alpha-linolenic acid, the bead dosage form made, it is added on and in feedstuff, have mould proof, moistureproof effect.
Claims (9)
1. a kind of Chinese medicine pre-mixing agent for anthelmintic, it is characterized by：6 to 10 tastes are selected to make by the Chinese medicine of following weight proportioning：River
5～10 parts of chinaberry, 60～90 parts of Semen Cucurbitae, 10～15 parts of Fructus Carotae, 3～9 parts of Fructus Mume, 30～60 parts of Herba polygoni hydropiperis, 2～5 parts of Rhizoma et radix veratri (Radix Rhizoma Veratri),
15～30 parts of Bulbus Allii, 3～15 parts of Pericarpium Granati, 30～60 parts of Herba Scutellariae Barbatae, 1.5～3 parts of Flos Genkwa, 3～10 parts of Spina Gleditsiae, Herba Artemisiae Annuae 5～
15 parts, 6～10 parts of Cortex Meliae, 3～10 parts of Semen Pharbitidiss, 1～3 part of Fructus Bruceae, 10～30 parts of Herba Gelsemii Elegantis, 0.3～1.5 part of Chalcanthitum, wolf
3～6 parts of poison, 3～10 parts of Semen Aesculi, 10～15 parts of Radix Dichroae.
2. a kind of Chinese medicine pre-mixing agent for anthelmintic as claimed in claim 1, it is characterized by：Preparation method is：
(1) Fructus Carotae extract, Herba polygoni hydropiperis extract, Herba Scutellariae Barbatae extract, Herba Gelsemii Elegantis extract, Radix Dichroae extract, Cortex Meliae is taken to carry
Take thing one or more, be mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 7-9 times of Chinese medicine gross weight boils 40-50 minute, filter, filtrate is concentrated
To the extractum of relative density 1.08-1.12, extractum adds ethanol, makes alcohol content reach 50～60%, refilters, then filtrate is concentrated
To the extractum of relative density 1.10-1.14, it is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, add the extract of pretreatment, Palmic acid, alpha-linolenic acid,
Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 20-30 minute, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:15-
20:15-20:8-12:4-6:10-15.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 20-30 minute,
Pelletize, obtain final product.
3. a kind of Chinese medicine pre-mixing agent for anthelmintic, it is characterized by：It is made up of the Chinese medicine of following weight proportioning：Fructus Toosendan 5～10
Part, 60～90 parts of Semen Cucurbitae, 10～15 parts of Fructus Carotae, 3～9 parts of Fructus Mume, 30～60 parts of Herba polygoni hydropiperis, 2～5 parts of Rhizoma et radix veratri (Radix Rhizoma Veratri), Bulbus Allii 15～
30 parts, 3～15 parts of Pericarpium Granati, 30～60 parts of Herba Scutellariae Barbatae.
4. a kind of Chinese medicine pre-mixing agent for anthelmintic as claimed in claim 3, it is characterized by：Preparation method is：
(1) take Fructus Carotae extract, Herba polygoni hydropiperis extract, Herba Scutellariae Barbatae extract, be mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 7-9 times of Chinese medicine gross weight boils 40-50 minute, filter, filtrate is concentrated
To the extractum of relative density 1.08-1.12, extractum adds ethanol, makes alcohol content reach 50～60%, refilters, then filtrate is concentrated
To the extractum of relative density 1.10-1.14, it is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, add the extract of pretreatment, Palmic acid, alpha-linolenic acid,
Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 20-30 minute, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:15-
20:15-20:8-12:4-6:10-15.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 20-30 minute,
Pelletize, obtain final product.
5. a kind of Chinese medicine pre-mixing agent for anthelmintic, it is characterized by：It is made up of the Chinese medicine of following weight proportioning：Flos Genkwa 1.5～3
Part, 3～10 parts of Spina Gleditsiae, 5～15 parts of Herba Artemisiae Annuae, 6～10 parts of Cortex Meliae, 3～10 parts of Semen Pharbitidiss, 1～3 part of Fructus Bruceae, Herba Gelsemii Elegantis 10
～30 parts, 0.3～1.5 part of Chalcanthitum, 3～6 parts of the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae), 3～10 parts of Semen Aesculi, 10～15 parts of Radix Dichroae.
6. a kind of Chinese medicine pre-mixing agent for anthelmintic as claimed in claim 5, it is characterized by：Preparation method is：
(1) hook taking kiss extract, Radix Dichroae extract, Cortex Meliae extract, are mixed into the extract of pretreatment, standby；
(2) take remaining Chinese medicine in formula, plus the decocting of 7-9 times of Chinese medicine gross weight boils 40-50 minute, filter, filtrate is concentrated
To the extractum of relative density 1.08-1.12, extractum adds ethanol, makes alcohol content reach 50～60%, refilters, then filtrate is concentrated
To the extractum of relative density 1.10-1.14, it is dried, obtains final product Chinese medicine extract；
(3) Chinese medicine extract taking 2/3 mass is placed in mixer, add the extract of pretreatment, Palmic acid, alpha-linolenic acid,
Pregelatinized Starch, dextrin, Microcrystalline Cellulose, mix 20-30 minute, standby；
Chinese medicine extract with the mass ratio of Palmic acid, alpha-linolenic acid, Pregelatinized Starch, dextrin, Microcrystalline Cellulose is：100:15-
20:15-20:8-12:4-6:10-15.
(4) take remaining Chinese medicine extract to be placed in another mixer, add step (3) resulting material, mix 20-30 minute,
Pelletize, obtain final product.
7. a kind of Chinese medicine pre-mixing agent for anthelmintic as described in claim 2 or 4, it is characterized by：
The preextraction of Fructus Carotae：By the Fructus Carotae boiling water heat-insulation soaking 10-14h of 9-11 times of quality, filter, obtain filtrate a；?
It is 1.04-1.06 that filtrate a is evaporated to relative density, obtains extractum；90% ethanol of extractum plus 2-3 times of volume, stir
Uniformly, filter to get filtrate b；The n-butanol extraction of filtrate b equal volume, separate and obtain n-butanol layer, the positive fourth of recovered under reduced pressure
Alcohol, is dried, obtains final product Fructus Carotae extract；
The preextraction of Herba polygoni hydropiperis：Herba polygoni hydropiperis are pulverized, boils 20-30 minute with 7-9 times of decocting, filter, obtain medicinal residues；Medicinal residues 6-
The dehydrated alcohol of 8 times of quality-ether mixed solution soaking at room temperature 4-6h, centrifugation, separate and obtain supernatant；Supernatant plus 2%
The activated carbon of quality, filters, obtains filtrate 1.；Filtrate 1. add 95% ethanol to be made alcohol content to be 70%, filters, obtain filtrate 2.,
2. filtrate be evaporated to no alcohol taste, is dried, obtains final product Herba polygoni hydropiperis extract；
Wherein in dehydrated alcohol-ether mixed solution, the volume of dehydrated alcohol and ether is 2:1；
The preextraction of Herba Scutellariae Barbatae：Herba Scutellariae Barbatae is pulverized, plus 70-80 DEG C of 75% ethanol of 14-16 times of quality is heated to reflux 180-
220min, filters, obtains filtrate；Upper for filtrate HZ002 ion exchange absorption resin column, upper column flow rate is 1.1-1.3BV/h, then uses
30% methanol -70% water of 3BV, 20% methanol -80% water of 2BV, 10% methanol -90% water of 1BV, gradient elution successively,
Elution flow rate is 1.1-1.3BV/h, collects and combines 30% methanol -70% water, 20% methanol -80% water, 10% methanol -90% water
Eluent, the 1/3 of eluent decompression and solvent recovery to original volume, is spray-dried, as Herba Scutellariae Barbatae extract；
Wherein, the volume ratio of filtrate and HZ002 ion exchange absorption resin is 2:1.
8. a kind of Chinese medicine pre-mixing agent for anthelmintic as described in claim 2 or 6, it is characterized by：
The preextraction of Herba Gelsemii Elegantis：Herba Gelsemii Elegantis are pulverized, with 40-50 DEG C of extraction 2- of n-hexane-ethyl acetate mixed liquor of 13-15 times of quality
3h, filters, obtains filtrate；It is 8-9 that filtrate is adjusted pH value, and centrifugation separates and obtains supernatant；Supernatant is evaporated to relatively
Density is the extractum of 1.11-1.13, is dried, obtains Herba Gelsemii Elegantis extract；
Wherein in n-hexane-ethyl acetate mixed liquor, the volume ratio of normal hexane and ethyl acetate is 1:3；
The preextraction of Radix Dichroae：Radix Dichroae is pulverized, plus the acetone supersound extraction 30-40 minute of 3-5 times of quality, filter, obtain filtering residue；?
Filtering residue adds 60-70 DEG C of heating and refluxing extraction 120-180min of methanol of 10-12 times of quality, filters, obtains filtrate；Upper for filtrate AB-8
Macroporous adsorptive resins, upper column flow rate is 1.5-1.7BV/h, then 40% second of 20% ethanol -80% acetone with 5BV, 3BV
Alcohol -60% acetone, 80% ethanol -20% acetone of 1BV, gradient elution successively, elution flow rate is 1.5-1.7BV/h, collects and closes
And 20% ethanol -80% acetone, 40% ethanol -60% acetone, 80% ethanol -20% acetone eluant, eluent is reduced pressure back
Receive to no alcohol taste, be spray-dried, as Radix Dichroae extract；
Wherein, the volume ratio of filtrate and AB-8 macroporous adsorbent resin is 2.5:1；
The preextraction of Cortex Meliae：Cortex Meliae is pulverized, plus the benzene of 10-12 times of quality-acetone and ethyl acetate mixed liquor 150W microwave
Extract 30 minutes, filter, obtain filtrate；Filtrate reduced in volume recycling design, it is dried, obtains final product Cortex Meliae extract；
Wherein, in benzene-acetone and ethyl acetate mixed liquor, benzene, acetone, the volume ratio of ethyl acetate are 2:3:4.
9. a kind of Chinese medicine pre-mixing agent for anthelmintic as described in claim 1 or 3 or 5, it is characterized by：
The using method of the present invention：1.0-1.5 kilogram of finished product, direct feeding is mixed thoroughly in feedstuff per ton.
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