CN108392527A - A kind of pest repellant and its production method suitable for livestock body surface - Google Patents
A kind of pest repellant and its production method suitable for livestock body surface Download PDFInfo
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- CN108392527A CN108392527A CN201810101103.5A CN201810101103A CN108392527A CN 108392527 A CN108392527 A CN 108392527A CN 201810101103 A CN201810101103 A CN 201810101103A CN 108392527 A CN108392527 A CN 108392527A
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- bentonite
- production method
- body surface
- pest repellant
- livestock
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/4164—1,3-Diazoles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/535—Perilla (beefsteak plant)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/58—Meliaceae (Chinaberry or Mahogany family), e.g. Azadirachta (neem)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/14—Ectoparasiticides, e.g. scabicides
Abstract
The present invention is a kind of pest repellant and its production method suitable for livestock body surface, which includes:1)Chinese herbal medicine, ethyl alcohol and ethyl acetate are decocted, then filter to take filtrate so that extracting solution is made;2)Under ultrasound condition, extracting solution, nano-bentonite, rotenone, Albendazole are subjected to adsorption treatment, then filtered so that modified Nano bentonite is made;3)Modified Nano bentonite, cyclodextrin and water are subjected to ultrasonic vibration so that the pest repellant for livestock body surface is made;Wherein, Chinese herbal medicine is azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, raw Radix Astragali, buckeye and cacumen biotae, perilla leaf.The pest repellant for being used for livestock body surface has excellent anthelminthic effect for the body surface of livestock.
Description
Technical field
The present invention relates to a kind of drugs, and in particular, to a kind of pest repellant and its production method suitable for livestock body surface.
Background technology
Animal husbandry refer to herd, stable breeding or the two combine mode, raising livestock and poultry to obtain animal product or the draught animal
Production division.It include livestock raise herd, home poultry raising, economic beasts are raised and train.Animal husbandry has important in national economy
Status and effect, predominantly:1. providing the animal foods such as meat, milk, eggs;2. for industry provide wool, cashmere, skin,
The raw materials such as hair on the neck, beast bone, casing;3. exporting acquisition of foreign currency by livestock products;4. the industry of animal husbandry input and livestock products is promoted to add
Industrial expansion increases labour employment chance;5. providing organic fertilizer for production estimation;6. increasing farmers' income;7. being agriculture
Industry and transportation provide animal power;8. promoting the economy and cultural development in vast pastoral area, strengthen the unity among nationalities.Thus
As it can be seen that the development of country is to be unable to do without the support of animal husbandry.
During developing animal husbandry, often need to livestock carry out expelling parasite processing, wherein be divided into internal expelling parasite and
Body surface area worm.Currently, body surface area worm is generally to carry out expelling parasite using chemically synthesized organic reagent, although can obtain certain
Anthelminthic effect;But on the one hand the health of livestock can be adversely affected by being chemical synthesis reagent, and then affect domestic animal
The meat and pelage quality of poultry, the chemically synthesized reagent of another aspect can cause environmental pollution.
Invention content
The object of the present invention is to provide a kind of pest repellants and its production method suitable for livestock body surface, this is used for livestock body
The pest repellant of table has excellent anthelminthic effect for the body surface of livestock, and milder, nuisanceless to environment and livestock;Together
When the production method have the advantages that process is simple and be easy to get with raw material.
To achieve the goals above, the present invention provides a kind of production methods of the pest repellant for livestock body surface, including:
1)Chinese herbal medicine, ethyl alcohol and ethyl acetate are decocted, then filter to take filtrate so that extracting solution is made;2)In ultrasound condition
Under, the extracting solution, nano-bentonite, rotenone, Albendazole are subjected to adsorption treatment, then filters and is received so that modification is made
Rice bentonite;3)The modified Nano bentonite, cyclodextrin and water are subjected to ultrasonic vibration and are used for livestock body surface to be made described
Pest repellant;Wherein, the Chinese herbal medicine is azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, raw Radix Astragali, buckeye, cacumen biotae, purple
Perilla leaf;In step 1)In, the weight ratio of the Chinese herbal medicine, ethyl alcohol and ethyl acetate is 10:35-45:10-15;In the medium-height grass
In medicine, the azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, raw Radix Astragali, buckeye, cacumen biotae, perilla leaf weight ratio be 10:
5-8:15-20:8-10:3-6:10-12:8-12:6-10.
Further, the decoction meets the following conditions:It is 85 DEG C to decoct temperature, decocting time 5h.
Wherein, in step 2)In, the extracting solution, nano-bentonite, rotenone, Albendazole weight ratio be 100:
45:10:8;In step 2)In, the adsorption treatment meets the following conditions:Treatment temperature is 55 DEG C, processing time 10h, ultrasound
Frequency is 35KHz.
Further, in step 3)In, the weight ratio of the modified Nano bentonite, cyclodextrin and water is 10:7:255;
Step 3)In, the ultrasonic vibration meets the following conditions:It is 45 DEG C to shake temperature, and the concussion time is 8h, and supersonic frequency is
48KHz。
Further, the average grain diameter of the nano-bentonite is 33nm.
The present invention also provides a kind of pest repellant for livestock body surface, which passes through above-mentioned
Production method be made.
In the above-mentioned technical solutions, the present invention passes through first by the mixed liquor of ethyl alcohol and ethyl acetate composition to Chinese herbal medicine
Decoct and then obtains extracting solution;Then, extracting solution, rotenone, Albendazole are adsorbed by nano-bentonite
And then modified Nano bentonite is made;Then, modified Nano bentonite is coated by cyclodextrin, while utilizes cyclodextrin
Outer rim is hydrophilic and characteristic that inner cavity is hydrophobic so as to soluble in water and then pest repellant for livestock body surface is made.The drive
Worm agent has excellent anthelminthic effect not only for the body surface of livestock, while milder, nuisanceless to environment and livestock;Together
When the production method have the advantages that process is simple and be easy to get with raw material.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Specific implementation mode
The specific implementation mode of the present invention is described in detail below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
The present invention provides a kind of production methods of the pest repellant for livestock body surface, including:
1)Chinese herbal medicine, ethyl alcohol and ethyl acetate are decocted, then filter to take filtrate so that extracting solution is made;
2)Under ultrasound condition, extracting solution, nano-bentonite, rotenone, Albendazole are subjected to adsorption treatment, then filtered
Modified Nano bentonite is made;
3)Modified Nano bentonite, cyclodextrin and water are subjected to ultrasonic vibration so that the pest repellant for livestock body surface is made;
Wherein, Chinese herbal medicine is azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, raw Radix Astragali, buckeye and cacumen biotae, perilla leaf.
In the step 1 of the present invention)In, the dosage of each material can select in a wide range, but in order to make the expelling parasite
Agent has superior anthelminthic effect and mildness, it is preferable that in step 1)In, Chinese herbal medicine, ethyl alcohol and ethyl acetate weight
Amount is than being 10:35-45:10-15;In Chinese herbal medicine, azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, raw Radix Astragali, buckeye and side
The weight ratio of CedarLeaves is 10:5-8:12-20:8-12:3-6:10-12:6-8:8-10.
In the step 1 of the present invention)In, the condition of decoction can select in a wide range, but in order to make the pest repellant
With superior anthelminthic effect and mildness, it is preferable that decoction meets the following conditions:It is 79-83 DEG C to decoct temperature, is decocted
Time is 2-4h.
In the step 2 of the present invention)In, the dosage of each substance can select in a wide range, but in order to make the expelling parasite
Agent has superior anthelminthic effect and mildness, it is preferable that in step 2)In, extracting solution, nano-bentonite, rotenone,
The weight ratio of Albendazole is 100:30-40:5-8:2-6.
In the step 2 of the present invention)In, the condition of adsorption treatment can select in a wide range, but in order to make the drive
Worm agent has superior anthelminthic effect and mildness, it is preferable that in step 2)In, adsorption treatment meets the following conditions:Place
It is 40-50 DEG C, processing time 6-8h, supersonic frequency 25-30KHz to manage temperature.
In the step 3 of the present invention)In, the dosage of each substance can select in a wide range, but in order to make the expelling parasite
Agent has superior anthelminthic effect and mildness, it is preferable that in step 3)In, modified Nano bentonite, cyclodextrin and water
Weight ratio be 10:4-6:200-220.
In the step 3 of the present invention)In, the condition of ultrasonic vibration can select in a wide range, but in order to make the drive
Worm agent has superior anthelminthic effect and mildness, it is preferable that in step 3)In, ultrasonic vibration meets the following conditions:Shake
It is 15-35 DEG C to swing temperature, and the concussion time is 3-5h, supersonic frequency 35-40KHz.
In the present invention, the average grain diameter of nano-bentonite can select in a wide range, but in order to make the expelling parasite
Agent has superior anthelminthic effect and mildness, it is preferable that the average grain diameter of nano-bentonite is 15-30nm.
The present invention also provides a kind of pest repellant for livestock body surface, which passes through above-mentioned
Production method be made.
The present invention will be described in detail by way of examples below.
Embodiment 1
1)By Chinese herbal medicine(Azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, the weight for giving birth to Radix Astragali, buckeye and cacumen biotae, perilla leaf
Than being 10:7:30:10:5:8:12:10), ethyl alcohol and ethyl acetate is according to 10:40:15 weight ratio is decocted(Decoct temperature
It is 80 DEG C, decocting time 3h), filtrate is then filtered to take so that extracting solution is made;
2)Under ultrasound condition, by extracting solution, nano-bentonite(Average grain diameter is 25nm), rotenone, Albendazole according to
100:35:7:5 weight ratio carries out adsorption treatment(Treatment temperature is 48 DEG C, processing time 9h, supersonic frequency 38KHz),
Then it filters so that modified Nano bentonite is made;
3)By modified Nano bentonite, cyclodextrin and water according to 10:8:210 weight ratio carries out ultrasonic vibration(Shaking temperature is
25 DEG C, the concussion time is 5h, supersonic frequency 44KHz)The pest repellant A1 for livestock body surface is made.
Embodiment 2
1)By Chinese herbal medicine(Azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, the weight for giving birth to Radix Astragali, buckeye and cacumen biotae, perilla leaf
Than being 10:7:13:8:3:10:8:9), ethyl alcohol and ethyl acetate is according to 10:35:10 weight ratio is decocted(Decocting temperature is
79 DEG C, decocting time 2h), filtrate is then filtered to take so that extracting solution is made;
2)Under ultrasound condition, by extracting solution, nano-bentonite(Average grain diameter is 15nm), rotenone, Albendazole according to
100:45:5:4 weight ratio carries out adsorption treatment(Treatment temperature is 40 DEG C, processing time 6h, supersonic frequency 25KHz),
Then it filters so that modified Nano bentonite is made;
3)By modified Nano bentonite, cyclodextrin and water according to 10:4:210 weight ratio carries out ultrasonic vibration(Shaking temperature is
15 DEG C, the concussion time is 3h, supersonic frequency 35KHz)The pest repellant A2 for livestock body surface is made.
Embodiment 3
1)By Chinese herbal medicine(Azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, the weight for giving birth to Radix Astragali, buckeye and cacumen biotae, perilla leaf
Than being 10:8:20:12:6:12:15:12), ethyl alcohol and ethyl acetate is according to 10:45:15 weight ratio is decocted(Decoct temperature
Degree is 83 DEG C, decocting time 4h), filtrate is then filtered to take so that extracting solution is made;
2)Under ultrasound condition, by extracting solution, nano-bentonite(Average grain diameter is 30nm), rotenone, Albendazole according to
100:55:8:10 weight ratio carries out adsorption treatment(Treatment temperature is 50 DEG C, processing time 8h, supersonic frequency 30KHz),
Then it filters so that modified Nano bentonite is made;
3)By modified Nano bentonite, cyclodextrin and water according to 10:8:240 weight ratio carries out ultrasonic vibration(Shaking temperature is
35 DEG C, the concussion time is 5h, supersonic frequency 48KHz)The pest repellant A3 for livestock body surface is made.
Comparative example 1
It carries out that pest repellant B1 is made according to the method for embodiment 1, unlike, step 2)Extracting solution is not used.
Comparative example 2
It carries out that pest repellant B2 is made according to the method for embodiment 1, unlike, step 3)Cyclodextrin is not used.
Detect example 1
Using flea as experiment made on the living in this test example, enough food is provided during experiment made on the living.6 groups of work are chosen respectively
Body flea(It is sexually matured), every group 10(Each half of male and female), above-mentioned pest repellant periodically then is sprayed to every group of flea, daily
Sprinkling 3 times, each every group of sprinkling 10mL, continuous sprinkling 7 days, after right the latter moon in every group live body flea quantity;Its is hollow
White control group spray water;Concrete outcome is shown in Table 1.
Table 1
The substance of sprinkling | After one month in every group live body flea quantity/only |
A1 | 0 |
A2 | 0 |
A3 | 0 |
B1 | 125 |
B2 | 33 |
Water | 342 |
The preferred embodiment of the present invention has been described above in detail, still, the tool during present invention is not limited to the embodiments described above
Body details can carry out a variety of simple variants, these letters to technical scheme of the present invention within the scope of the technical concept of the present invention
Monotropic type all belongs to the scope of protection of the present invention.In addition, can also be appointed between a variety of different embodiments of the present invention
Meaning combination, as long as it does not violate the idea of the present invention, it should also be regarded as the disclosure of the present invention.Additionally need explanation
, specific technical features described in the above specific embodiments in the case of no contradiction can be by appointing
What suitable mode is combined, in order to avoid unnecessary repetition, the present invention to various combinations of possible ways no longer separately
Explanation.
Claims (8)
1. a kind of production method of pest repellant for livestock body surface, which is characterized in that including:
1)Chinese herbal medicine, ethyl alcohol and ethyl acetate are decocted, then filter to take filtrate so that extracting solution is made;2)In ultrasound condition
Under, the extracting solution, nano-bentonite, rotenone, Albendazole are subjected to adsorption treatment, then filters and is received so that modification is made
Rice bentonite;3)The modified Nano bentonite, cyclodextrin and water are subjected to ultrasonic vibration and are used for livestock body surface to be made described
Pest repellant;Wherein, the Chinese herbal medicine is azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, raw Radix Astragali, buckeye, cacumen biotae, purple
Perilla leaf;In step 1)In, the weight ratio of the Chinese herbal medicine, ethyl alcohol and ethyl acetate is 10:35-45:10-15;In the medium-height grass
In medicine, the azedarach, radix ranunculi ternati, manyleaf meadowure rhizome and root, semen momordicae, raw Radix Astragali, buckeye, cacumen biotae, perilla leaf weight ratio be 10:
5-8:15-20:8-10:3-6:10-12:8-12:6-10.
2. production method according to claim 1, wherein the decoction meets the following conditions:It is 85 DEG C to decoct temperature, is decocted
It is 5h to boil the time.
3. according to the production method described in any one of claim 1-2, wherein in step 2)In, the extracting solution, nanometer
Bentonite, rotenone, Albendazole weight ratio be 100:45:10:8.
4. production method according to claim 3, wherein in step 2)In, the adsorption treatment meets the following conditions:Place
It is 55 DEG C, processing time 10h, supersonic frequency 35KHz to manage temperature.
5. according to the production method described in claim 1-2, any one of 4, wherein in step 3)In, the modified Nano
The weight ratio of bentonite, cyclodextrin and water is 10:7:255.
6. according to the production method described in claim 1-2, any one of 4, wherein in step 3)In, the ultrasonic vibration
Meet the following conditions:It is 45 DEG C to shake temperature, and the concussion time is 8h, supersonic frequency 48KHz.
7. according to the production method described in claim 1-2, any one of 4, wherein the average grain diameter of the nano-bentonite
For 33nm.
8. a kind of pest repellant for livestock body surface, which is characterized in that the pest repellant for livestock body surface is wanted by right
The production method described in any one of 1-7 is asked to be made.
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CN102100661A (en) * | 2009-12-17 | 2011-06-22 | 苏州知微堂生物科技有限公司 | Process for preparing novel formulation of integrative traditional Chinese medicine and production method thereof |
CN103341053A (en) * | 2013-07-26 | 2013-10-09 | 广东药学院 | Purification method for large ligusticum wallichii oral liquid |
CN105418217A (en) * | 2015-11-27 | 2016-03-23 | 颍上县永祥旱粮研究所 | Corn compound fertilizer with slow-release and insect-resistant effects |
CN106377656A (en) * | 2016-10-21 | 2017-02-08 | 江西正邦动物保健品有限公司 | Traditional Chinese medicine pre-mixing agent for expelling parasites and preparation method thereof |
CN106490026A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of storage pest insecticide and preparation method thereof |
CN106857726A (en) * | 2017-01-23 | 2017-06-20 | 嵊州市派特普科技开发有限公司 | Pure natural insecticide and preparation method thereof |
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2018
- 2018-02-01 CN CN201810101103.5A patent/CN108392527A/en not_active Withdrawn
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102100661A (en) * | 2009-12-17 | 2011-06-22 | 苏州知微堂生物科技有限公司 | Process for preparing novel formulation of integrative traditional Chinese medicine and production method thereof |
CN101732336A (en) * | 2009-12-18 | 2010-06-16 | 华中师范大学 | Antiparasitic solution for external use |
CN103341053A (en) * | 2013-07-26 | 2013-10-09 | 广东药学院 | Purification method for large ligusticum wallichii oral liquid |
CN105418217A (en) * | 2015-11-27 | 2016-03-23 | 颍上县永祥旱粮研究所 | Corn compound fertilizer with slow-release and insect-resistant effects |
CN106377656A (en) * | 2016-10-21 | 2017-02-08 | 江西正邦动物保健品有限公司 | Traditional Chinese medicine pre-mixing agent for expelling parasites and preparation method thereof |
CN106490026A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of storage pest insecticide and preparation method thereof |
CN106857726A (en) * | 2017-01-23 | 2017-06-20 | 嵊州市派特普科技开发有限公司 | Pure natural insecticide and preparation method thereof |
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