CN105941313A - Anser cygnoides high hatching rate hatching method - Google Patents
Anser cygnoides high hatching rate hatching method Download PDFInfo
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- CN105941313A CN105941313A CN201610324588.5A CN201610324588A CN105941313A CN 105941313 A CN105941313 A CN 105941313A CN 201610324588 A CN201610324588 A CN 201610324588A CN 105941313 A CN105941313 A CN 105941313A
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- 230000012447 hatching Effects 0.000 title claims abstract description 74
- 241000272816 Anser cygnoides Species 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 19
- 235000013601 eggs Nutrition 0.000 claims abstract description 35
- 238000011534 incubation Methods 0.000 claims abstract description 30
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- 239000004575 stone Substances 0.000 claims description 11
- 239000006071 cream Substances 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 241000272814 Anser sp. Species 0.000 claims description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 6
- 241000756943 Codonopsis Species 0.000 claims description 6
- 241000628997 Flos Species 0.000 claims description 6
- 241000209094 Oryza Species 0.000 claims description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims description 6
- 239000010231 banlangen Substances 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 238000009395 breeding Methods 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 239000012634 fragment Substances 0.000 claims description 6
- 239000010903 husk Substances 0.000 claims description 6
- 235000009566 rice Nutrition 0.000 claims description 6
- 239000004576 sand Substances 0.000 claims description 6
- 238000009210 therapy by ultrasound Methods 0.000 claims description 6
- 239000002023 wood Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- 239000009636 Huang Qi Substances 0.000 claims description 3
- 241000245240 Lonicera Species 0.000 claims description 3
- LTGPFZWZZNUIIK-LURJTMIESA-N Lysol Chemical compound NCCCC[C@H](N)CO LTGPFZWZZNUIIK-LURJTMIESA-N 0.000 claims description 3
- -1 Maifanitum Substances 0.000 claims description 3
- 241000545442 Radix Species 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 3
- 239000001569 carbon dioxide Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 238000002604 ultrasonography Methods 0.000 claims description 3
- 241000272808 Anser Species 0.000 abstract 1
- 238000011177 media preparation Methods 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000037396 body weight Effects 0.000 description 2
- 230000008175 fetal development Effects 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000271566 Aves Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 244000273256 Phragmites communis Species 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 241000238370 Sepia Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 230000001617 migratory effect Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses an anser cygnoides high hatching rate hatching method comprising the following steps: 1, hatching egg disinfection; 2, hatching medium preparation; 3, hatching egg incubation; 4, hatching egg spawned. The hatching method can carry out scientific, reasonable and refinement management for hatching conditions of the hatching eggs, can comply with the anser cygnoides hatching egg hatching rules, thus obviously improving hatching rate, and the final hatching rate can be above 94%; the hatched young ansers are strong in body, and active in spirit.
Description
Technical field
The invention belongs to cultivation field, be specifically related to the hatching method of a kind of swan goose high hatchability.
Background technology
Swan goose (formal name used at school: swan goose, Anser cygnoides) is large-scale aquatic bird, body length 90 cm, body weight 2.8-5 kilogram.Mouth black, body colour terra brown, the dark sepia in the crown to collare, foreneck near-white.Seeming the crown and collare black, foreneck near-white at a distance, black-and-white two color is clearly demarcated, and contrast is strong.Mainly inhabit the lake on open plain and meadow, Plain, pool, river, marsh and in the neighbourhood.With various herbal leaves, bud, include that the plant food such as terrestrial plant and water plant, phragmites communis, algae, for food, also eats the animal foods such as a small amount of shell-fish and Mollusca.Property happiness knot group, Chang Chengqun is movable, particularly migratory seasons, the jumpbogroup that Chang Jicheng is tens of, hundreds of, the most thousands of.
There is the saying of " rather eat birds flatly, do not eat beast half jin " in China from ancient times, and swan goose body is loose, the most single body weight 2.5 ~ 3.5 kilograms, big up to more than 4.5 kilograms；Swan goose is good at long-distance circling in the air, and chest, leg muscle are the most flourishing, and meat is abundant, though fiber is relatively thick, but meat flavour is delicious, has a burst of delicate fragrance after the cooking, top grade in game.Owing to ecological environment destroys day by day, adding that people hunt excessively, the wild resource of swan goose is fewer and feweri, and increasing people starts artificial incubation and raises and train.But being limited to current hatching technique, the artificial incubation rate of swan goose is only about 80%, the raising of incubation rate is an up a key factor of artificial cultivation benefit, is also the problem needing now solution badly.
Summary of the invention
In order to solve the problems referred to above, the invention provides the hatching method of a kind of swan goose high hatchability, have the advantages that incubation rate wild goose body constitution high, young is good.
The present invention is achieved through the following technical solutions:
The hatching method of a kind of swan goose high hatchability, comprises the steps:
(1) hatching egg sterilization: swan goose hatching egg is put in the lysol aqueous solution that mass percent concentration is 3 ~ 5% immersion 20 ~ 30min, then takes out and rinse well with clear water, standby after cleaning surface moisture；
(2) prepared by incubation medium:
A. the material of following weight portion is weighed: 10 ~ 15 portions of wood fragments bits, 5 ~ 8 portions of rice husks, 10 ~ 15 portions of fluvial sand, 3 ~ 5 portions of Maifanitums, 2 ~ 3 portions of stone needles, 3 ~ 5 portions of Radixs Astragali, 2 ~ 4 portions of Radix Isatidis, 2 ~ 4 portions of Flos Loniceraes, 1 ~ 2 portion of Herba Hedyotidis Diffusae, 3 ~ 4 portions of Fructus Lyciis, 2 ~ 3 portions of Radix Codonopsis, 1 ~ 3 portion of Radix Ranunculi Ternati；
B. the Radix Astragali in mentioned component, Radix Isatidis, Flos Lonicerae, Herba Hedyotidis Diffusae, Fructus Lycii, Radix Codonopsis, Radix Ranunculi Ternati are put in the clear water of its gross mass 2 ~ 3 times, heating boiling filters to get filtrate after boiling 30 ~ 40min, filtrate carries out distillation and is dried that to make Chinese medicine extraction cream powder standby；
C. incubation medium is i.e. obtained after wood fragments bits, rice husk, fluvial sand, Maifanitum, stone needle ibid being walked prepared Chinese medicine extraction cream powder mix homogeneously；
(3) hatching of breeding eggs:
A. the hatching egg after sterilization is placed in incubation medium, it is desirable in 1/3 volume embedment incubation medium of hatching egg, the amount of residue 2/3 exposes in atmosphere；
B. the 1st ~ 7 day hatched, the temperature controlling hatching is 38.2 DEG C, and humidity is 60 ~ 63%, 8th ~ 14 day, the temperature controlling hatching is 38.0 DEG C, and humidity is the 63 ~ 66%, the 15th ~ 21 day, the temperature controlling hatching is 37.8 DEG C, humidity is 66 ~ 70%, and after the 22nd day, the temperature controlling hatching is 37.6 DEG C, humidity is 70 ~ 73%, and the oxygen content controlling air during whole hatching is 20.5 ~ 21.5%, carbon dioxide content is below 0.4%；Carry out temperature and humidity during hatching of breeding eggs refining control, use suitable humiture in different phase, speed and the quality of hatching can be promoted；
C. the 8th day hatched starts, every day egg-turning twice, the most in the morning 8.00 and carry out evening 8.00, still require that after egg-turning that in 1/3 volume embedment incubation medium of hatching egg, the amount of residue 2/3 exposes in atmosphere；
D. the 8th day hatched, to the 21st day, carries out the ultrasonic Treatment operation of 2 weeks by a definite date, carries out twice every day, and the most in the morning 10.00 and carry out evening 10.00, process 2 ~ 3min every time；
(4) hatching egg is hatched: during young wild goose broken shell, and not artificial assistance broken shell allows it naturally go out shell, is drawn off laying after going out shell.
Further, described in step (2), the granular size of stone needle is 2 ~ 4mm.
Further, the frequency of step (3) operation ultrasound wave described in d is 25 ~ 28KHz.
There is advantages that
(1) the cream powder extracted from Chinese medicine added in this incubation medium, it is possible not only to play the effect of sterilizing, purifies environment of hatching, reduce hatching egg and corroded dead probability by pathogenic bacteria, certain nutrient substance can be provided for hatching egg again, improve vigor and the resistance of embryo；The Maifanitum added can absorb the harmful substance that fetal development metabolism goes out, and purifies environment of hatching, improves quality and the success rate of hatching；The stone needle added contains and has special energy, the blood microcirculation of embryo in hatching egg, conditioning metabolism can be promoted, promote that it is hatched, promote incubation rate.
(2) during the 8th day hatched was to 21 days, namely the fetal development stage faster, hatching egg is carried out ultrasonic Treatment, can well strengthen the activity of various enzyme, promote the synthesis of material, improve incubation rate；Carry out all can not reaching optimal effect too early or too late, and the frequency of ultrasonic Treatment can not be excessive with effect duration, otherwise can play reverse effect.
(3) this hatching method has carried out scientific and reasonable ZOOM analysis to the incubation condition of hatching egg, meets the rule of swan goose hatching of breeding eggs, has been obviously improved incubation rate, and final incubation rate is up to more than 94%, and the young wild goose brought out has, and body constitution is strong, the feature of prothymia.
Detailed description of the invention
Embodiment 1
The hatching method of a kind of swan goose high hatchability, comprises the steps:
(1) hatching egg sterilization: swan goose hatching egg is put in the lysol aqueous solution that mass percent concentration is 3 ~ 5% immersion 20 ~ 30min, then takes out and rinse well with clear water, standby after cleaning surface moisture；
(2) prepared by incubation medium:
A. the material of following weight portion is weighed: 10 ~ 15 portions of wood fragments bits, 5 ~ 8 portions of rice husks, 10 ~ 15 portions of fluvial sand, 3 ~ 5 portions of Maifanitums, 2 ~ 3 portions of stone needles, 3 ~ 5 portions of Radixs Astragali, 2 ~ 4 portions of Radix Isatidis, 2 ~ 4 portions of Flos Loniceraes, 1 ~ 2 portion of Herba Hedyotidis Diffusae, 3 ~ 4 portions of Fructus Lyciis, 2 ~ 3 portions of Radix Codonopsis, 1 ~ 3 portion of Radix Ranunculi Ternati；
B. the Radix Astragali in mentioned component, Radix Isatidis, Flos Lonicerae, Herba Hedyotidis Diffusae, Fructus Lycii, Radix Codonopsis, Radix Ranunculi Ternati are put in the clear water of its gross mass 2 ~ 3 times, heating boiling filters to get filtrate after boiling 30 ~ 40min, filtrate carries out distillation and is dried that to make Chinese medicine extraction cream powder standby；
C. incubation medium is i.e. obtained after wood fragments bits, rice husk, fluvial sand, Maifanitum, stone needle ibid being walked prepared Chinese medicine extraction cream powder mix homogeneously；
(3) hatching of breeding eggs:
A. the hatching egg after sterilization is placed in incubation medium, it is desirable in 1/3 volume embedment incubation medium of hatching egg, the amount of residue 2/3 exposes in atmosphere；
B. the 1st ~ 7 day hatched, the temperature controlling hatching is 38.2 DEG C, and humidity is 60 ~ 63%, 8th ~ 14 day, the temperature controlling hatching is 38.0 DEG C, and humidity is the 63 ~ 66%, the 15th ~ 21 day, the temperature controlling hatching is 37.8 DEG C, humidity is 66 ~ 70%, and after the 22nd day, the temperature controlling hatching is 37.6 DEG C, humidity is 70 ~ 73%, and the oxygen content controlling air during whole hatching is 20.5 ~ 21.5%, carbon dioxide content is below 0.4%；
C. the 8th day hatched starts, every day egg-turning twice, the most in the morning 8.00 and carry out evening 8.00, still require that after egg-turning that in 1/3 volume embedment incubation medium of hatching egg, the amount of residue 2/3 exposes in atmosphere；
D. the 8th day hatched, to the 21st day, carries out the ultrasonic Treatment operation of 2 weeks by a definite date, carries out twice every day, and the most in the morning 10.00 and carry out evening 10.00, process 2 ~ 3min every time；
(4) hatching egg is hatched: during young wild goose broken shell, and not artificial assistance broken shell allows it naturally go out shell, is drawn off laying after going out shell.
Further, described in step (2), the granular size of stone needle is 2 ~ 4mm.
Further, the frequency of step (3) operation ultrasound wave described in d is 25 ~ 28KHz.
Comparative example 1
Compared with Example 1, in addition to not containing stone needle in incubation medium, remaining method step is the most identical for this comparative example 1.
Comparative example 2
It compared with Example 1, except the hatching of breeding eggs stage, is not carried out outside ultrasonic Treatment by this comparative example 2, and remaining method step is the most identical.
Matched group
Common swan goose artificial incubation method.
Choosing with a batch of swan goose hatching egg, be divided into four groups at random, carry out artificial incubation by above-mentioned four kinds of methods respectively, its Statistical Comparison data are as follows:
| Embodiment 1 | Comparative example 1 | Comparative example 2 | Matched group | |
| Incubation rate (%) | 95.4 | 90.2 | 91.4 | 82.6 |
| Brooding time (my god) | 27 | 28 | 28 | 31 |
| Young wild goose state | Spirit is very well | Spirit is preferably | Spirit is preferably | Spirit is poor |
Claims (3)
1. the hatching method of a swan goose high hatchability, it is characterised in that comprise the steps:
(1) hatching egg sterilization: swan goose hatching egg is put in the lysol aqueous solution that mass percent concentration is 3 ~ 5% immersion 20 ~ 30min, then takes out and rinse well with clear water, standby after cleaning surface moisture；
(2) prepared by incubation medium:
A. the material of following weight portion is weighed: 10 ~ 15 portions of wood fragments bits, 5 ~ 8 portions of rice husks, 10 ~ 15 portions of fluvial sand, 3 ~ 5 portions of Maifanitums, 2 ~ 3 portions of stone needles, 3 ~ 5 portions of Radixs Astragali, 2 ~ 4 portions of Radix Isatidis, 2 ~ 4 portions of Flos Loniceraes, 1 ~ 2 portion of Herba Hedyotidis Diffusae, 3 ~ 4 portions of Fructus Lyciis, 2 ~ 3 portions of Radix Codonopsis, 1 ~ 3 portion of Radix Ranunculi Ternati；
B. the Radix Astragali in mentioned component, Radix Isatidis, Flos Lonicerae, Herba Hedyotidis Diffusae, Fructus Lycii, Radix Codonopsis, Radix Ranunculi Ternati are put in the clear water of its gross mass 2 ~ 3 times, heating boiling filters to get filtrate after boiling 30 ~ 40min, filtrate carries out distillation and is dried that to make Chinese medicine extraction cream powder standby；
C. incubation medium is i.e. obtained after wood fragments bits, rice husk, fluvial sand, Maifanitum, stone needle ibid being walked prepared Chinese medicine extraction cream powder mix homogeneously；
(3) hatching of breeding eggs:
A. the hatching egg after sterilization is placed in incubation medium, it is desirable in 1/3 volume embedment incubation medium of hatching egg, the amount of residue 2/3 exposes in atmosphere；
B. the 1st ~ 7 day hatched, the temperature controlling hatching is 38.2 DEG C, and humidity is 60 ~ 63%, 8th ~ 14 day, the temperature controlling hatching is 38.0 DEG C, and humidity is the 63 ~ 66%, the 15th ~ 21 day, the temperature controlling hatching is 37.8 DEG C, humidity is 66 ~ 70%, and after the 22nd day, the temperature controlling hatching is 37.6 DEG C, humidity is 70 ~ 73%, and the oxygen content controlling air during whole hatching is 20.5 ~ 21.5%, carbon dioxide content is below 0.4%；
C. the 8th day hatched starts, every day egg-turning twice, the most in the morning 8.00 and carry out evening 8.00, still require that after egg-turning that in 1/3 volume embedment incubation medium of hatching egg, the amount of residue 2/3 exposes in atmosphere；
D. the 8th day hatched, to the 21st day, carries out the ultrasonic Treatment operation of 2 weeks by a definite date, carries out twice every day, and the most in the morning 10.00 and carry out evening 10.00, process 2 ~ 3min every time；
(4) hatching egg is hatched: during young wild goose broken shell, and not artificial assistance broken shell allows it naturally go out shell, is drawn off laying after going out shell.
The hatching method of a kind of swan goose high hatchability the most according to claim 1, it is characterised in that described in step (2), the granular size of stone needle is 2 ~ 4mm.
The hatching method of a kind of swan goose high hatchability the most according to claim 1, it is characterised in that the frequency of step (3) operation ultrasound wave described in d is 25 ~ 28KHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610324588.5A CN105941313A (en) | 2016-05-17 | 2016-05-17 | Anser cygnoides high hatching rate hatching method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610324588.5A CN105941313A (en) | 2016-05-17 | 2016-05-17 | Anser cygnoides high hatching rate hatching method |
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| CN105941313A true CN105941313A (en) | 2016-09-21 |
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| CN201610324588.5A Pending CN105941313A (en) | 2016-05-17 | 2016-05-17 | Anser cygnoides high hatching rate hatching method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107258687A (en) * | 2017-08-17 | 2017-10-20 | 合肥市明航养殖有限公司 | A kind of method for improving duck's egg incubation rate |
| CN107372333A (en) * | 2017-08-17 | 2017-11-24 | 合肥市明航养殖有限公司 | A kind of high-survival rate hatching method of duck's egg |
| CN107372786A (en) * | 2017-08-17 | 2017-11-24 | 合肥市明航养殖有限公司 | A kind of store method of high hatchability kind duck's egg |
| CN108835040A (en) * | 2018-07-12 | 2018-11-20 | 湖北富山生态农业股份有限公司 | A kind of pigeon eggs artificial incubation method |
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| US4893585A (en) * | 1986-11-06 | 1990-01-16 | Ethyl Corporation | Zeolites in poultry hatching |
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| CN103141436A (en) * | 2013-03-08 | 2013-06-12 | 固镇县淮鸿灰天鹅养殖专业合作社 | Method for hatching eggs of swan geese |
| CN103355248A (en) * | 2013-07-09 | 2013-10-23 | 江苏圣农禽业发展有限公司 | Hatching method of hatching eggs of poultry |
| CN105123615A (en) * | 2015-09-07 | 2015-12-09 | 中国科学院西北高原生物研究所 | Artificial variable temperature incubation method suitable for species bar-headed gooses endemic to Qinghai-Tibet Plateau |
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2016
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| US4893585A (en) * | 1986-11-06 | 1990-01-16 | Ethyl Corporation | Zeolites in poultry hatching |
| CN101103705A (en) * | 2007-08-01 | 2008-01-16 | 陈永南 | Method for hatching fowl eggs |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107258687A (en) * | 2017-08-17 | 2017-10-20 | 合肥市明航养殖有限公司 | A kind of method for improving duck's egg incubation rate |
| CN107372333A (en) * | 2017-08-17 | 2017-11-24 | 合肥市明航养殖有限公司 | A kind of high-survival rate hatching method of duck's egg |
| CN107372786A (en) * | 2017-08-17 | 2017-11-24 | 合肥市明航养殖有限公司 | A kind of store method of high hatchability kind duck's egg |
| CN108835040A (en) * | 2018-07-12 | 2018-11-20 | 湖北富山生态农业股份有限公司 | A kind of pigeon eggs artificial incubation method |
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Application publication date: 20160921 |