JP2009209057A - Immunoactivator and its manufacturing method, and foodstuff for human being, fodder for animal or domestic animal, immunoactivator for plant, and cosmetic raw material, each comprising the immunoactivator - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an immunoactivator that exhibits practical immunoactivating effects. <P>SOLUTION: Four bacterial groups consisting of Lactobacilli, saccharomyces, pichia and bacilli, or five bacterial groups further containing actinomyces in addition to the four bacterial groups are cultivated in a nutrient medium containing rice bran, water and saccharides to generate a fungus seed. The cultivation is carried out by means of aerating symbiotic cultivation in the nutrient medium using glucose as the saccharide to give a culture solution. The culture solution is then subjected to a sterilization step, a filtration step and the like for purifying the immunoactivating agent. Then, the immunoactivating agent is incorporated with a substrate for foods by a spray-drying method or an immersion method to give a dietary supplement and the like. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an immunoactive agent and a method for producing the same, and foods for humans including health foods and supplements containing the immunoactive agent, animal and livestock feeds, plant immunoactive agents, and cosmetic raw materials.

  Living organisms such as humans, animals and plants maintain their health and sustain their growth by their immunity, antioxidant power, antiallergic power and the like. Immunoactive agents that enhance immunity and have an antioxidant action are extremely valuable.

In the prior art, various supplements have been developed that are said to enhance immunity and have an antioxidant effect.
For example, various animal feeds have been developed, but with the recent pet boom, feeds that take into account the health of animals are commercially available, and some of them are used to cure animal diseases. Animal feeds that are effective in improving immunity have also been developed.

For example, the following patent documents have been made as patent applications intended to improve immunity.
Japanese Patent Application Laid-Open No. 2003-531597 is cited as Patent Document 1. The said patent document 1 is a patent application regarding the feed containing garlic.
Japanese Patent Application Laid-Open No. 2001-112436 is cited as Patent Document 2. The said patent document 2 is a patent application regarding the feed and pet food containing a basidiomycete.
Japanese Patent Application Laid-Open No. 11-89539 is cited as Patent Document 3. The said patent document 3 is a patent application regarding the feed, foodstuff, etc. which contain the leaf etc. of a stevia plant.

JP 2003-531597 A JP 2001-112436 A JP-A-11-89539

However, although Patent Documents 1 to 3 describe that an effect of improving immunity can be obtained, in actuality, it is mainly intended for edible use of domestic animals, and is intended to provide an immune activity effect. It was n’t. In addition, the usefulness of the immune activity effect is not clear.
Currently, animal and livestock feeds, etc. that are traded on the market are only edible for animals, and no animal or livestock feeds that are effective for immune activity have been developed yet.

Many things have been developed, such as supplements that increase human immunity and supplements based on lactic acid bacteria that have an intestinal regulating effect, but they not only enhance immunity but also antioxidant, antiallergic and intestinal regulating effects There were no supplements at the same time.
Therefore, the present invention provides an immunoactive agent effective for an immune activity effect.

The present invention has been made to solve such problems.
The inventor (Seiichi Takeyasu) collected soil from various locations for many years and found a plurality of fungal groups with very high activity on plants. Furthermore, the inventor has repeatedly cultured these plural fungal groups in a medium containing rice bran, water, and glucose, and has come to obtain an inoculum in a state where these plural fungal groups coexist.
As a result of earnest research, the inventor has established a liquid aeration co-cultivation method using a fungal group in which these fungal groups coexist, and a storage method for these fungal groups has not been changed for a long time. Established.

  It was found that an immunoactive agent produced by liquid aeration co-cultivation and purifying the culture solution has a high immune activity effect on useful microorganisms in soil, plants, animals / livestock, and humans.

The immunoactive agent of the present invention can be contained in various substances.
For example, if a soil activator prepared by adding glucose to a cellulose base is added, good immune activity can be imparted to useful microorganisms in the soil. In addition, as a result of inventor's diligent research, by adding glucose to the cellulose base material, it becomes pseudo close to the root of the plant and glucose supplied from the root. It was found that it can provide an environment that coexists with the roots of the tree.

  Moreover, for example, it became possible to provide the plant immunoactive agent which raises the immunity of a plant by making it contain in the plant immunoactive agent.

  In addition, for example, by containing the immunoactive agent of the present invention in animal / livestock feeds or supplements for animals, it is excellent in immune response and can directly contribute to the immune activity effect of animals. It has become possible to provide an immunoactive agent, a method for producing the same, an animal / livestock feed and an animal supplement containing the animal immunoactive agent.

  In addition, for example, by adding the immunoactive agent of the present invention to human foods, etc., human immunity is enhanced, and human foods such as health foods and supplements that simultaneously work with antioxidant, antiallergic and intestinal regulating effects. It became possible to provide food.

  Furthermore, by mixing the immunoactive agent of the present invention into a cosmetic raw material, it has become possible to enhance the immunity of the user's skin and the like and to provide a cosmetic raw material having an antioxidant action and an antiallergic action.

The immunoactive agent of the present invention is obtained by culturing a group of bacteria containing the following bacteria in a medium containing rice bran, water, and sugars.
1. 1. Lactobacillus group including Lactobacillus or Lactobacillus and Lactobacillus and similar bacteria 2. Saccharomyces fungi or Saccharomyces fungi, including Saccharomyces fungi and fungi of the same kind as Saccharomyces fungi 3. Pichia group including Pichia or Pichia and Pichia and the like. Bacillus group including Bacillus or Bacillus and Bacillus and similar groups

  In addition, the said fungal group is a state in which the four types of bacterial groups coexist by repeating culture on the four types of bacterial groups of the Lactobacillus group, the Saccharomyces group, the Pichia group, and the Bacillus group. Is preferably obtained by aeration and co-cultivation of the symbiotic bacteria group in the medium.

Next, the immunoactive agent of the present invention is an immunoactive agent characterized by being obtained by culturing a bacterial group containing the following bacteria in a medium containing rice bran, water, and sugars.
1. 1. Lactobacillus group including Lactobacillus or Lactobacillus and Lactobacillus and similar bacteria 2. Saccharomyces fungi or Saccharomyces fungi, including Saccharomyces fungi and fungi of the same kind as Saccharomyces fungi 3. Pichia group including Pichia or Pichia and Pichia and the like. 4. Bacillus group including Bacillus or Bacillus and Bacillus and the like Actinomycetes, or actinomycetes, including actinomycetes and similar fungi

  In addition, as the fungal group, the five types of fungal groups are obtained by repeating the culture on the five types of fungal groups of the Lactobacillus group, the Saccharomyces group, the Pichia group, the Bacillus group, and the Actinomyces group. It is preferable to use a symbiotic state, and to obtain the symbiotic group of bacteria by aeration and co-cultivation in the medium.

  Next, the soil activator of the present invention is a soil activator prepared by adding glucose to a cellulose base containing the immunoactive agent according to the present invention, which is good against useful microorganisms in the soil. It is characterized by providing an environment in which the useful microorganisms in the soil are symbiotically symbiotic to the roots of the plant by adding the glucose to the cellulose base material while imparting a good immune activity.

  Next, the health supplement for pets of the present invention is a health supplement for pets prepared by containing the immunoactive agent according to the present invention in a food base material. It is characterized by being able to improve activity and ingest nutritional supplements.

  Next, the animal / livestock feed of the present invention is an animal / livestock feed prepared by containing the immunoactive agent according to the present invention in a food base material, and the animal・ Improvement of livestock immune activity and intake of nutritional components.

  Next, the human supplement of the present invention is a human supplement prepared by containing the immunoactive agent according to the present invention in a food base material, which improves the immune activity of the user by oral ingestion and the nutritional supplement component. It is characterized by its ingestion.

  Next, the cosmetic raw material of the present invention is a cosmetic raw material prepared by mixing the immunoactive agent according to the present invention, characterized in that the immune activity can be improved and moisturized by skin absorption by rubbing into the skin. To do.

Next, the method for producing the immunoactive agent of the present invention comprises:
1. 1. Lactobacillus group including Lactobacillus or Lactobacillus and Lactobacillus and similar bacteria 2. Saccharomyces fungi or Saccharomyces fungi, including Saccharomyces fungi and fungi of the same kind as Saccharomyces fungi 3. Pichia group including Pichia or Pichia and Pichia and the like. Bacillus bacteria, or Bacillus bacteria group including the same fungal group of Bacillus bacteria and Bacillus bacteria, and repeating the culture for the above four types of bacterial groups to produce a state in which the four types of bacterial groups coexist,
A method for producing an immunoactive agent, comprising purifying the immunoactive agent by subjecting the symbiotic group of bacteria to aeration co-cultivation in a medium containing rice bran, water, and sugars.

Next, the method for producing the immunoactive agent of the present invention comprises:
1. 1. Lactobacillus group including Lactobacillus or Lactobacillus and Lactobacillus and similar bacteria 2. Saccharomyces fungi or Saccharomyces fungi, including Saccharomyces fungi and fungi of the same kind as Saccharomyces fungi 3. Pichia group including Pichia or Pichia and Pichia and the like. 4. Bacillus group including Bacillus or Bacillus and Bacillus and the like Actinomycetes, or actinomycetes group containing actinomycetes and fungi of the same type as actinomycetes, and repeating the culture for the above five types of fungal groups to produce a state in which the five types of fungal groups coexist,
A method for producing an immunoactive agent, comprising purifying the immunoactive agent by subjecting the symbiotic group of bacteria to aeration co-cultivation in a medium containing rice bran, water, and sugars.

One method of incorporating the immunoactive agent of the present invention into various food bases is a spray drying method. The spray drying method is a method in which an immunoactive agent is contained in a food base material by spraying and drying the immunoactive agent of the present invention on the food base material.
One method for incorporating the immunoactive agent of the present invention into various food bases is an immersion method. The dipping method is a method in which a food base material contains an immunoactive agent by immersing the food base material in the immunoactive agent of the present invention.

  According to the present invention, human immunoactive agents having an excellent immune activity promoting action against humans and animals, animal / livestock immunoactive agents, and human foods and supplements containing such human immunoactive agents, and It has become possible to provide animal / livestock feeds, animal / livestock supplements, plant immune activators and cosmetic ingredients containing animal / livestock immunoactive agents.

  Hereinafter, with reference to the drawings, examples of the immunoactive agent of the present invention and the production method thereof, human food, animal / livestock feed, plant immunoactive agent and cosmetic raw materials containing the immunoactive agent will be described. To do. However, it goes without saying that the scope of the present invention is not limited to the specific applications, shapes, numerical values, numbers, etc. shown in the following examples.

The immunoactive agent of the present invention according to Example 1 is a medium containing rice bran, water, and sugar, and includes four types of bacterial groups including Lactobacillus group, Saccharomyces group, Pichia group, Bacillus group, or It is characterized by being obtained by culturing 5 types of fungal groups including the actinomycete group in addition to these 4 types of fungal groups. In particular, in Example 1, a culture in which four types of bacterial groups or five types of bacterial groups are cultivated by repeating culture on these four types of bacterial groups or five types of bacterial groups is used. Obtained by aeration co-cultivation in a medium.
Although the kind of rice bran which is a composition component of a culture medium is not specifically limited, For example, what was generated when brown rice was polished can be used. Further, glucose may be a commonly used one. The water may be ordinary water, for example, tap water (well water).

  The above-mentioned Lactobacillus group, Saccharomyces group, Pichia group, Bacillus group, Actinomycetes group are originally useful soil fungal groups in nature, but these four or five fungal groups coexisted together. There was nothing in state. Moreover, the technique which obtains a culture solution by carrying out liquid aeration culture | cultivation using these 4 microbe groups or 5 microbe groups is not established by persons other than this inventor. Therefore, it is necessary to devise a culture medium and a culture method. As a result of inventor Seiichi Takeyasu's research in Yonago City in December 1990, these four fungal groups or five fungal groups remain in a state of cohabitation. A technique for obtaining a culture solution in an aerated state was established for the first time. In the present invention, a method of obtaining a culture solution in a liquid aerated state in a state where these four fungal groups or five types of fungal groups coexist in a medium obtained from rice bran, water, and glucose is referred to as “liquid aeration using useful fungal groups”. It was named “Kyoto culture”.

The production process of the medium of the immunoactive agent of the present invention by liquid aeration co-cultivation using a useful fungus group includes a mixing process of rice bran and water, a filtration process after mixing, a process of adding glucose to the mixed solution (filtrate), (Replenishment step of water to the filtrate) and sterilization step.
FIG. 1 is a diagram showing the flow of the above-described medium production process.
The production process of the culture solution of the immunoactive agent of the present invention by liquid aeration co-cultivation using useful fungal groups includes the steps of inoculating the above-mentioned medium with 4 or 5 bacterial groups, and primary culture by aeration culture. It consists of a process, a secondary culture process by cold storage, and a sterilization process.
FIG. 2 is a diagram showing a flow of the above-mentioned culture solution process.
The above culture solution is the immunologically active agent (stock solution) of the present invention, and is prepared as a useful agent by being contained in various base materials according to uses such as for humans, animals / livestock and plants.

[Collection of useful bacteria]
Inventor Seiichi Takeyasu collected useful soil bacteria from soil near the hillside at an altitude of 1500m at the prefectural border between Tottori and Hyogo Prefectures. Succeeded in generating. It was found that the inoculum contained a useful soil fungal group including a Lactobacillus group, a Saccharomyces group, a Pichia group, a Bacillus group, and an actinomycete group in a stable and coexisting state.

[Production of medium]
In the production of the culture medium, first, the rice bran was mixed with 5% equivalent amount of raw rice bran and stirred to extract an aqueous solution of rice bran. Next, this rice bran mixture was filtered with a cotton cloth. In addition to the cotton cloth, for example, a suction machine or a filter press can be used for this filtration.
Next, water is replenished to the above aqueous solution, 5% of the total amount of glucose is added, sterilized at 121 ° C. for 17 minutes, and then cooled to prepare a medium.

[Manufacture of culture solution]
In the production of the culture solution, a small amount of cells are taken from a group of bacteria in which four types of bacteria or a group of bacteria containing five types of bacteria are in a state of cohabitation with the medium, and the medium is inoculated.
A solution inoculated with a small amount of cells taken out from this group of bacteria was aerated at 37 ° C. for 48 hours.
Next, after aeration culture, cold storage was performed at 5 ° C. for 1 month. After cold storage, it was sterilized at 121 ° C. for 17 minutes to obtain a stock solution of an immunoactive agent as a culture solution. This culture solution was refrigerated at 5 ° C. or lower.

[Preparation process of useful agent]
After passing through a filtration step that removes the cells as needed from the stock solution of the immunoactive agent that has been stored at low temperature or after passing through the filtration step without removing the cells, it is added to the base material. It was processed into liquids, powders and solids and prepared as useful agents for humans, animals / livestock and plants.

  Hereinafter, as Example 2 to Example 6, an immunoactive agent of the present invention is contained in a base material, processed into a liquid, powder or solid form, and used as a useful agent for humans, animals / livestock and plants. A prepared example will be described.

  As Example 2, an example will be described in which the culture solution prepared in Example 1 is contained in a cellulose base material, and glucose is further added to form a soil activator.

  Originally, a part of the starch produced by photosynthesis of plant leaves is secreted from the roots as glucose, and useful microorganisms in the soil proliferate using this glucose as a nutrient source. These useful microorganisms in the soil produce plant growth promoting substances, self-protective power against pests, and immune active substances that give natural healing power. Plants are said to have a symbiotic relationship that absorbs these trace substances from the roots and preserves their own health. That is, a favorable soil state is a state in which useful microorganisms in the soil are symbiotic with the roots of the plant.

  The soil activator according to Example 2 is intended to create a pseudo state in which useful microorganisms in the soil coexist with the roots of the plant. Therefore, a soil activator prepared by adding glucose to a cellulose base containing the immunoactive agent of the present invention was used. That is, the soil activator of Example 2 simulates the state in which the glucose of the plant and glucose secreted from the root exist by adding glucose to the cellulose base material containing the immunoactive agent of the present invention. It is made to give to useful microorganisms in the soil, and the useful microorganisms in the soil are easily symbiotic in a good soil condition.

For many years, the present inventor has provided the soil activator of Example 2 to the soil and has confirmed its efficacy on plants.
By providing the soil activator of Example 2 to the soil, as an effect of enhancing immunity, it is difficult to get ill, and it is difficult for mold and pests to be damaged even when the amount of fungicides and insecticides used is small. became. Further, as a growth promoting effect, growth promotion of leaf vegetables (spinach, Japanese mustard spinach, green pepper etc.) was observed. In addition, the effect of promoting root growth was also observed. In addition, there is a tendency that the rate of fruiting increases and the actual size tends to increase.

  As Example 3, an example in which a pet food supplement prepared by containing the immunoactive agent prepared in Example 1 in a pet food will be described. It is a health supplement for pets that can improve pet immune activity and ingest nutritional supplements by ingesting pets.

  What is necessary is just to use the pet food according to an animal and livestock suitably. What is necessary is just to mix | blend the immunoactive agent prepared in Example 1 with respect to pet food. The blending amount may be adjusted according to animals and livestock, but it may be blended so that an immunoactive agent equivalent to 1 / 60,000 of the pet's body weight can be ingested in a pet food that a pet eats a day.

Hereinafter, an example in which the immune activity effect of animals / livestock by administering the health supplement for pets of Example 3 was obtained will be shown. The effect was confirmed using dogs, which are familiar animals as pets, as an example.
[Table 1] below shows the results of immune activation of the dog's pet health supplement of Example 3.

  As is apparent from the results shown in Table 1 above, the improvement of various dermatitis, including allergic dermatitis, takes about one month after the start of the administration of the pet health supplement for dogs of this Example 3. It turns out that an effect is seen. Moreover, the result that the glossiness improved and the bad smell of urine was relieved was also obtained. It turns out that it can be expected sufficiently as an adjuvant for dog skin diseases.

Next, as another verification example, the veterinarian cooperated under confidentiality to verify the symptom improvement effect of administration of the health supplement for pets of this Example 3 to dogs with allergic otitis externa. Received.
[Table 2] below shows the results of immune activation of the dog's pet health supplement of Example 3. The subject dog was a poodle and a 1 year old male.

  As is clear from the results shown in Table 2 above, an improvement effect on allergic otitis externa is observed about one month after the start of the administration of the dog's pet health supplement in Example 3 I understand. Immune activity was confirmed in clinical experiments in the presence of veterinarians.

Next, as another verification example, the symptom improvement effect by administration of the pet supplement of this Example 3 with respect to the dog with allergic dermatitis was verified.
The symptom improvement effect by administration of the health supplement for pets of this Example 3 with respect to the dog with allergic dermatitis is shown in FIG. 3, FIG. The subject dog was a Dalmatian, a 10 year old male weighing 20 kg.

Fig.3 (a) is the photograph of the back part in which allergic dermatitis before administration of the health supplement for pets of this Example 3 is seen. As shown in FIG. 3 (a), the hair in the center of the back fell off, and the reddish skin was exposed.
Administration of the health supplement for pets of Example 3 (2 g per day) was continued for 5 weeks.

  FIG. 3 (b) and FIG. 4 are photographs of the back part in which allergic dermatitis improved after the administration of the pet supplement of Example 3 for 5 weeks. As shown in FIG. 3B and FIG. 4, the redness of the skin at the center of the back where the hair had fallen off disappeared and the hair grew again. In addition, when allergic dermatitis of FIG. 3 (a) and FIG. 4 had developed, it was unstable because the dog was not settled down due to itching, but the allergic dermatitis of FIG. 3 (b) and FIG. After healed, the itch disappeared and the dog calmed down and returned to normal.

As Example 4, an example in which animal and livestock feed prepared by adding the immunoactive agent prepared in Example 1 to bran or soybean meal will be described. Example 4 is an animal / livestock feed that can improve the immune activity and intake nutrient components by ingestion of animals / livestock.
The animal / livestock feed may be appropriately selected according to the animal / livestock, but in Example 4, bran was used. The immunoactive agent prepared in Example 1 is blended with bran. The blending amount may be adjusted according to the animal / livestock, but about 20% of the animal / livestock feed is sufficient.

  Hereinafter, an example in which the immune activity effect of animals and livestock by feeding the animal and livestock feed of Example 4 was obtained will be shown. The effects were confirmed by taking dairy cows, pigs and chickens as domestic animals as examples. As a monitor, the following dairy farmers cooperated to confirm the effect.

Dairy cows are dairy farmers in Sumoto City, Hyogo Prefecture (bred 80 dairy cows), dairy farmers in Izumo City, Shimane Prefecture (bred 60 dairy cows), dairy farmers in Yasugi City, Shimane Prefecture (bred 60 dairy cows), etc. We received cooperation from dairy farmers.
Wagyu was supported by the Yoshida fattening center in Yoshida Village, Shimane Prefecture (bred 250 wagyu beef), the dairy farmer in Misumi-cho, Otsu-gun, Yamaguchi (bred 20 wagyu beef), and other dairymen.
The pigs were cooperated by dairymen in Misumi-cho, Otsu-gun, Yamaguchi Prefecture (bred 1,000 pigs) and other dairymen.
The chickens were cooperated by dairymen in Sakaiminato City, Tottori Prefecture (raising 20,000 laying hens) and other dairymen.

The following reports were obtained from the dairy farmers who became monitors.
The following effects are common among dairy cows, Japanese beef, pigs and laying hens.
(1) The disease rate decreased and the amount of antibiotics used decreased.
(2) Prevention of diarrhea in young animals decreased.
(3) Reproductive disorders decreased, and the progress before and after delivery became better.
(4) Feces, urine, and barn scents drastically decreased, and flies were reduced.
(5) Compost has good fertilizing effect and smell is reduced

Next, the following reports on dairy cows were obtained.
(1) The digestive efficiency of dietary fiber in the feed was improved, and appetite increased.
(2) Milk yield increased and milk fat percentage improved.
(3) The mastitis decreased (somatic cell count decreased), and the symptoms became lighter.

Next, the following report was obtained regarding Wagyu beef.
(1) The digestive efficiency of dietary fiber in the feed was improved, and appetite increased.
(2) The cold and diarrhea at the time of introduction of the original cow decreased.
(3) Obstacles before fattening cattle were shipped decreased.

Next, the following report was obtained on pigs.
(1) The breeding rate of pigs and piglets improved.

Next, the following report was obtained regarding laying hens.
(1) Fewer diarrheal stools.
(2) Feces, urine, and barn odor decreased.
(3) Flies and maggots were reduced.
(4) Improvement in egg quality was observed.

  As described above, by feeding the animal / livestock feed of the present invention, an immune activity effect of the animal / livestock was obtained.

  As Example 5, an example of a human dietary supplement prepared by containing the immunoactive agent prepared in Example 1 will be described. The human dietary supplement of Example 5 can improve the immune activity of the user and ingest the nutritional supplement component when the user takes it orally.

As a human dietary supplement, any food supplement provided in the market as a human dietary supplement can be applied, and the immunoactive agent prepared in Example 1 was contained.
For example, the immunoactive agent prepared in Example 1 is added to a flour by spray drying and processed as a powder. Moreover, it can also shape | mold by granulating this powdery body with solid agents, such as dextrin. The blending amount may be prepared, but in Example 5, the same amount of the immunoactive agent is sprayed on the same amount of 1 g of the flour so that 1 g of the immunoactive agent of the present invention can be ingested in one bag, and dried. Can be easily ingested for 1 day by packing it in a stick bag.
It was verified by taking 58 monitors for 1 to 3 months.
The verification results are summarized in [Table 3].

  From the results of the human nutritional supplement intake experiment results from the above monitor, not only can the human immunostimulatory effect be obtained, but the human dietary supplement of Example 5 has an antioxidant action, an antiallergic action, and an intestinal action. I also found out that

  As Example 6, an example of a cosmetic raw material prepared by adding the immunoactive agent prepared in Example 1 to a moisturizing cream will be described. The cosmetic raw material prepared by adding the immunoactive agent prepared in Example 1 to a moisturizing cream enables improvement of the immunoactivity and moisturization by skin absorption by rubbing into the skin.

The cosmetic raw material according to Example 6 was prepared by containing 30% to 40% by weight of the immunoactive agent prepared in Example 1 with respect to the moisturizing cream. The above moisturizing cream was prepared by a cosmetic company in Osaka city.
It was verified by applying it to the skin on more than 50 monitors for 1 to 3 months.
The verification results are summarized in [Table 4].

  According to the results of the moisturizing cream application experiment by the above monitor, the immunoactive agent of the present invention is absorbed from human skin by application to the skin, and the immunostimulatory effect of human skin is obtained, and the antioxidative action and antiallergic action I also found out that It was also found that it promotes blood flow in the tissue under the skin and activates skin cells and muscle cells.

  It should be noted that the moisturizing cream containing the immunoactive agent of the present invention is absorbed from the skin of the animal by application to the skin to the animal, and has an immunostimulatory effect on the skin of the animal, and has an antioxidant action and an antiallergic action I have confirmed that I have it.

  For example, when the moisturizing cream of Example 5 was applied to a cow whose inflammation had developed in the nipple, the inflammation of the nipple was cured. FIG. 5 is a photograph comparing the state before and after the application of the moisturizing cream of Example 5 for cattle with inflammation on the nipple. It is clear that the inflammation in the nipple is clearly healed.

  FIG. 6 is a photograph comparing the state of the site where inflammation was observed before application of the moisturizing cream of Example 6 with respect to the cat having inflammation on the skin and the state after the inflammation was cured by application. Clearly, the inflammation that had occurred in the cat's skin was clearly healed. In addition to this example, a number of clinical trials in which inflammation on the skin of pet dogs / cats and nipples of domestic cows was cured were confirmed.

  As a result of the above-described experimental results of application of moisturizing cream to animals and livestock, the immunoactive agent of the present invention is absorbed from the skin when applied to the skin of animals and livestock, and an immunostimulatory effect on the skin of animals and livestock is obtained. It was also found to have oxidative and antiallergic effects. It was also found that it promotes blood flow in the tissue under the skin and activates skin cells and muscle cells.

  While preferred embodiments of the invention have been illustrated and described, it will be appreciated that various changes can be made without departing from the scope of the invention. Therefore, the technical scope of the present invention is limited only by the description of the appended claims.

  The immunoactive agent of the present invention and its production method, and human foods and supplements, animal samples, animal supplements and cosmetic ingredients containing the immunoactive agent are used to reduce the disease by increasing the immune effect of humans and animals. It can create a constitution that is difficult to become, and contributes greatly to maintaining the health of people and animals. In addition, it is effective not only for humans and animals but also for plant immunity. It is also effective for strengthening the immunity of plants, suppresses the use of excessive pesticides, and preserves the original taste and color of plants. it can.

  In addition, it has anti-oxidant, anti-allergic, and intestinal regulating functions, has excellent permeability, promotes skin blood flow, and enhances skin and muscle metabolism. There are also external effects such as pain and rubbing. Moreover, it activates useful microorganisms in the intestine and is very effective in improving constipation, diarrhea, liver function, and odor, and can be used for these preventive drugs.

Flow chart showing the production method of the culture medium Flow diagram showing the method for producing a mixed culture Figure showing how allergic dermatitis has been improved by the administration of health supplements for pets (Part 1) Diagram showing the improvement of allergic dermatitis by the administration of health supplements for pets (Part 2) The figure which shows that the inflammation which was made in the nipple of the cow was improved by feeding animal and livestock feed A figure showing how inflammation on the skin of animals and livestock has been improved by applying moisturizing cream

Claims (13)

An immunoactive agent obtained by culturing a group of bacteria containing the following bacteria in a medium containing rice bran, water, and sugar.
1. 1. Lactobacillus group including Lactobacillus or Lactobacillus and Lactobacillus and similar bacteria 2. Saccharomyces fungi or Saccharomyces fungi, including Saccharomyces fungi and fungi of the same kind as Saccharomyces fungi 3. Pichia group including Pichia or Pichia and Pichia and the like. Bacillus group including Bacillus or Bacillus and Bacillus and similar groups Using glucose as the saccharide,
As the fungal group, the culture of the four types of fungal groups of the Lactobacillus group, the Saccharomyces group, the Pichia group, and the Bacillus group is repeated and the four types of bacteria group are stably co-resident. Create things,
The immunologically active agent according to claim 1, which is obtained by aeration and co-cultivation of the symbiotic bacteria group in the medium. An immunoactive agent obtained by culturing a group of bacteria containing the following bacteria in a medium containing rice bran, water, and sugar.
1. 1. Lactobacillus group including Lactobacillus or Lactobacillus and Lactobacillus and similar bacteria 2. Saccharomyces fungi or Saccharomyces fungi, including Saccharomyces fungi and fungi of the same kind as Saccharomyces fungi 3. Pichia group including Pichia or Pichia and Pichia and the like. 4. Bacillus group including Bacillus or Bacillus and Bacillus and the like Actinomycetes, or actinomycetes, including actinomycetes and similar fungi Using glucose as the saccharide,
As the fungal group, the culture of the five types of the bacterial group is stable by repeating the culture on the five types of the bacterial group of the Lactobacillus group, the Saccharomyces group, the Pichia group, the Bacillus group, and the actinomycetes. Create a symbiotic state,
The immunologically active agent according to claim 1, which is obtained by aeration and co-cultivation of the symbiotic bacteria group in the medium.   A soil activator prepared by adding glucose to a cellulosic substrate containing the immunoactivator according to any one of claims 1 to 4 and having good immunity against useful microorganisms in soil A soil activator characterized by providing an environment in which the useful microorganisms in the soil are symbiotic with plant roots by adding the glucose to the cellulose base material while imparting activity.   A health supplement for pets prepared by containing the immunoactive agent according to any one of claims 1 to 4 in a food base material, wherein the pet's immune activity is improved by oral intake of the pet. A health supplement for pets, characterized in that the intake of nutritional supplements is possible.   An animal / livestock feed prepared by containing the immunoactive agent according to any one of claims 1 to 4 in a food base material, wherein the animal / livestock is immunized by oral intake of the animal / livestock. Animal and livestock feed characterized by improved activity and nutritional intake.   A human supplement prepared by containing the immunoactive agent according to any one of claims 1 to 4 in a food base material, and improving the immune activity of a user and ingesting a nutritional supplement by ingestion. A human supplement characterized by the fact that it is possible.   A cosmetic raw material prepared by containing the immunoactive agent according to any one of claims 1 to 4 in a cosmetic base material, which can improve immune activity and moisturize by skin absorption by rubbing into the skin. Cosmetic raw material characterized by that. Repeat the culture for the following four types of fungus group to make a state in which the four types of fungus group coexist,
A method for producing an immunoactive agent, comprising purifying the immunoactive agent by subjecting the symbiotic group of bacteria to aeration co-cultivation in a medium containing rice bran, water, and sugars.
1. 1. Lactobacillus group including Lactobacillus or Lactobacillus and Lactobacillus and similar bacteria 2. Saccharomyces fungi or Saccharomyces fungi, including Saccharomyces fungi and fungi of the same kind as Saccharomyces fungi 3. Pichia group including Pichia or Pichia and Pichia and the like. Bacillus group including Bacillus or Bacillus and Bacillus and similar groups Repeat the culture for the following 5 types of fungus group to make a state in which the 5 types of fungus group coexist,
A method for producing an immunoactive agent, comprising purifying the immunoactive agent by subjecting the symbiotic group of bacteria to aeration co-cultivation in a medium containing rice bran, water, and sugars.
1. 1. Lactobacillus group including Lactobacillus or Lactobacillus and Lactobacillus and similar bacteria 2. Saccharomyces fungi or Saccharomyces fungi, including Saccharomyces fungi and fungi of the same kind as Saccharomyces fungi 3. Pichia group including Pichia or Pichia and Pichia and the like. 4. Bacillus group including Bacillus or Bacillus and Bacillus and the like Actinomycetes, or actinomycetes, including actinomycetes and similar fungi   The manufacturing method of the immunoactive agent of Claim 9 or 10 which makes the said food base material contain the said immunoactive agent by spraying and drying the said immunoactive agent with respect to a food base material.   The manufacturing method of the immunoactive agent of Claim 9 or 10 which makes the said food base material contain the said immunoactive agent by immersing a food base material in the said immunoactive agent.

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