CN109805204A - A kind of compound bacteria fermented feed and preparation method thereof - Google Patents

A kind of compound bacteria fermented feed and preparation method thereof Download PDF

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CN109805204A
CN109805204A CN201910081227.6A CN201910081227A CN109805204A CN 109805204 A CN109805204 A CN 109805204A CN 201910081227 A CN201910081227 A CN 201910081227A CN 109805204 A CN109805204 A CN 109805204A
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weight
parts
fermentation
wet basis
meal
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孙春华
李鑫
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Shandong New Hope Liuhe Group Co Ltd
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Shandong New Hope Liuhe Group Co Ltd
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    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract

The invention discloses a kind of compound bacteria fermented feed and preparation method thereof, the feed includes following raw material: corn flour, wheat bran, peanut meal, seawood meal, rice bran, wet basis fermentation raw material, fermentation adjuvant, antioxidant and yeast culture;Preparation method includes: destruction step, batching step, granulation step and classification step.The beneficial effects of the present invention are: compound bacteria fermented feed provided by the invention can enhance the immunity of laying hen, can be obviously improved the conversion ratio of feed and the egg production of laying hen and no pollution to the environment.

Description

A kind of compound bacteria fermented feed and preparation method thereof
Technical field
The present invention relates to feed fermentation technology, in particular to a kind of compound bacteria fermented feed and preparation method thereof.
Background technique
Obtained mycoplasma contains the metabolite of thallus after solid fermentation, and mycoplasma itself is also good nutrients Matter.The mycoplasma and thallus that solid fermentation obtains are with nutrition and medicinal function, in the development and application side of natural feed addictive Face has a good application prospect.
Feed additive industry also obtains development at full speed with the booming of China's cultivation industry, dynamic in order to improve Object growth performance, reduces aquaculture cost, including antibiotic, and the feeds such as hormone quasi-growth promoter (Clenbuterol and start ketone etc.) add Add agent once or continue to be used widely, they are made that huge contribution to the development of animal husbandry.But such feed addition Also mouth benefit is significantly exposed the negative effect that agent is long-term, largely uses.It is in particular in: livestock and poultry and human body drug resistance It generating, the substances such as antibiotic are formed in body and are remained, animal immunizing power decline, many adverse reactions of human body generation etc., and 2001 The outlet obstructions main cause that Livestock Products in China is met with is exactly the residual of antibiotic.
Currently, internal feed additives industries are in a transitional period, low-residual or noresidue, without drug resistance, natural Green feed additive will gradually replace antibiotic and other feed addictives with residual contamination problem.Green feed adds Add agent, it is necessary to which having " can enhance the immunity of animal, can be obviously improved the conversion ratio of feed and the quantum of output of animal and to environment It is pollution-free " etc. basic demands.The use of green feed additive is the basis for ensureing animal food safety.It meets feed and adds Add the developing direction of agent, green feed additive will become the main product in feed addictive market, green feed additive Use the primary condition for being guarantee China's meat products safety.
Currently, the green feed additive in China is also developed, active bacteria formulation, saccharicter-penin, oligosaccharide, feeding enzyme system Agent, Chinese herbal feed additive are main several.There are product standards to lack, technical level is inclined for green feed additive at present Low, the problems such as product function ingredient is indefinite, additive amount is big.It is stable and controllable for quality, noresidue, non-environmental-pollution, using it is safe, It is not limited by raw material, the stable green feed additive product of properties of product is in urgent need of development.
How to solve the above problems, is then the project that the present invention is faced.
Summary of the invention
On the basis of nutritional need in order to meet laying cycle of laying hens, realize produced in chicken alimentary canal protease ability it is high, It is remarkably improved egg feedstuff protein digestibility, can be applied in egg feedstuff, Egg Production of Laying Hens later period production performance is improved And egg quality, while having the function of reducing the cholesterol level of laying hen egg, the immunity of laying hen can be enhanced, can obviously be changed The conversion ratio of kind feed and the egg production and no pollution to the environment of laying hen, the present invention provides a kind of compound bacteria fermented feed and its Preparation method.
In order to achieve the above-mentioned object of the invention, the present invention provides a kind of compound bacteria fermented feeds, wherein the feed includes Following raw material: corn flour, wheat bran, peanut meal, seawood meal, rice bran, wet basis fermentation raw material, fermentation adjuvant, antioxidant and Yeast culture.
Wherein, in the feed each raw material parts by weight are as follows: corn flour 20-40 parts by weight, wheat bran 12-24 weight Part, peanut meal 10-32 parts by weight, seawood meal 7-18 parts by weight, rice bran 12-35 parts by weight, wet basis fermentation raw material 14-28 weight Part, fermentation adjuvant 8-26 parts by weight, antioxidant 0.3-0.5 parts by weight and yeast culture 0.2-1.0 parts by weight.
It wherein, further include Chinese herb feed additive, parts by weight in the feed are as follows: corn flour 20-40 parts by weight, wheat Bran 12-24 parts by weight, peanut meal 10-32 parts by weight, seawood meal 7-18 parts by weight, rice bran 12-35 parts by weight, wet basis fermentation raw material 14-28 parts by weight, fermentation adjuvant 8-26 parts by weight, antioxidant 0.3-0.5 parts by weight, yeast culture 0.2-1.0 weight Part and Chinese herb feed additive 0.32-0.48 parts by weight.
Wherein, in the feed each raw material parts by weight are as follows: 35 parts by weight of corn flour, 22 parts by weight of wheat bran, peanut 30 parts by weight of the dregs of rice, 14 parts by weight of seawood meal, 28 parts by weight of rice bran, 20 parts by weight of wet basis fermentation raw material, fermentation 12 weight of adjuvant Part, 0.32 parts by weight of 0.4 parts by weight of antioxidant, 0.5 parts by weight of yeast culture and Chinese herb feed additive.
Wherein, in the Chinese herb feed additive each raw medicinal material parts by weight are as follows: root of Dahurain angelica 6-12 parts by weight, weeping forsythia leaf 4-8 parts by weight, caulis lonicerae 5-20 parts by weight, ganoderma lucidum 5-14 parts by weight, chamomile 10-25 parts by weight, pueraria lobata 8-16 parts by weight and Fleece-flower root 15-30 parts by weight.
In order to preferably realize foregoing invention purpose, the present invention also provides a kind of compound bacteria fermented feed, the medium-height grass Medicine additive is pulvis, application method are as follows: after the Chinese herb feed additive is processed into pulvis, with other feedstuffs or powder Other feedstuffs after broken are uniformly mixed, feeding after feeding or granulation.
In order to preferably realize foregoing invention purpose, the present invention also provides a kind of preparation sides of compound bacteria fermented feed Method, the Chinese herb feed additive are pulvis, preparation method are as follows:
The first step mixes the score ratio by weight of each raw medicinal material in the Chinese herb feed additive, and investment pulverizer carries out powder Broken or direct chopping is fitted into and decocts in container, and suitable quantity of water, which is added, makes it submerge medicinal material, after impregnating 30-50 minutes, is heated to boiling After 30-50 minutes, fried liquid is separated, the dregs of a decoction add water decoct 2-3 times again, respectively are isolated by fried liquid every time, merge institute The fried liquid having takes filtrate to abandon filter residue after filtering;
Second step, liquid cooling to be filtered but after, be added relative to 1-2 times of liquid weight after concentration determining alcohol be 80-90% ethyl alcohol, fill Divide and be uniformly mixed, stands overnight, obtain filtrate after so that its precipitating, next day is taken its supernatant liquid filtering, sediment is 50- with determining alcohol 60% ethyl alcohol is cleaned, and is collected washing lotion and is merged with filtrate, after ethyl alcohol is withdrawn in decompression, stands 0.5-1 hours, makes precipitating completely, mistake Filter, filtrate low-temperature evaporation obtain paste;
Step 3: paste placed into spray dryer it is dry, guarantee inlet air temperature at 180 DEG C -200 DEG C, leaving air temp 70 DEG C -90 DEG C, it is then ground into -300 mesh of 200 mesh, obtains pulvis.
Preferably, the wet basis fermentation raw material is that wet basis fermentation cotton dregs, wet basis ferment rapeseed meal, wet basis fermented peanut meal, wet Any composition in base fermentation palm kernel meal and wet basis fermentation chrysanthemum meal;The wet basis fermentation raw material is made of following methods:
(1) prepared by fermentation substrate: taking wet basis fermentation cotton dregs, wet basis fermentation rapeseed meal, wet basis fermented peanut meal, wet basis fermentation palm kernel meal With wet basis fermentation chrysanthemum meal 75%~90%, water 20%~40%, mix to get fermentation substrate;
(2) prepared by mixed bacteria liquid: taking bacillus subtilis bacterium solution 1.5-3.2 parts by weight, bacillus licheniformis liquid 2.5-4.2 weight Number, lactobacillus suspension 1.2-4.0 parts by weight and the mixing of saccharomycete 1.5-3.2 parts by weight liquid are to get mixed bacteria liquid;
(3) mixed bacteria liquid obtained by step (2) is taken to be inoculated on fermentation substrate made from step (1), inoculum concentration is fermentation substrate weight The 3%~25% of amount mixes, and ferments 96 hours at 22~37 DEG C to get wet basis fermentation raw material;
Wherein, each number of live bacteria of probiotics in the mixed bacteria liquid are as follows: bacillus subtilis 3.6-12.8 × 108CFU/g, lichens bud Spore bacillus 1.3-4.5 × 108CFU/g, lactic acid bacteria 1.4-5.2 × 108CFU/g and saccharomycete 1.2-7.7 × 108CFU/g;
In the wet basis fermentation raw material, moisture be 38.0~60.0%, bacillus subtilis bacterial content be 2.5 × 109~6 × 109cfu/g, bacillus licheniformis content are 1.5 × 109~3 × 109cfu/g, the content of lactic acid bacteria is 2 × 109~5 × 109cfu/g, the content of saccharomycete are 2.2 × 109~3.5 × 109cfu/g, and total acid content is 4~10%.
Preferably, the fermentation adjuvant is the preparation method comprises the following steps: be added to 1500 milliliter of 30 0C's for fermenting 3 grams of adjuvant It in warm water, stirs, dissolution, after dissolution, places and activate within 2 hours spare, 40-70 grams of bacterium solution, urea after then taking activation 2-7 kilograms, 2-6 kilograms of salt, be put into 1200-2000 kilograms of cylinder or Chi Zhongjia water, stir evenly, then activated strain liquid It dissolves in water, fermentation adjuvant is made.
In order to preferably realize foregoing invention purpose, the present invention also provides a kind of preparation sides of compound bacteria fermented feed Method, wherein the preparation method includes:
(1) destruction step: weighing corn flour, wheat bran, peanut meal, seawood meal, rice bran by weight percentage, investment pulverizer into Row Crushing of Ultrafine, by smashing it through 2.0mm sieve;
(2) batching step: according to parts by weight than weighing fermentation adjuvant, antioxidant, yeast culture and Chinese herbal medicine addition Agent crushes the fine powder obtained with step (1) and mixes, put into mixing machine, is uniformly mixed, incorporation time 20-30s;
(3) granulation step: investment granulator is pelletized, and pelleting temperature is 70 DEG C, its gelatinization degree is made to reach 90% or more;
(4) sieve classification classification step: was crushed after granulation again.
Wherein, in the step (4), the upper mesh size of the classification is 1.5-3.0mm, and lower mesh size is 1.0-2.0mm.
Technical solution provided by the invention has the benefit that compound bacteria fermented feed provided by the invention, is applicable in Not only meet the nutritional need of laying cycle of laying hens in the compound bacteria fermented feed of laying hen, but also reduces the cholesterol of laying hen egg Content improves the quality of egg;Additionally it is possible to promote the development of laying hen immune organ, the immunity of body is improved, is stimulated Body of gland discharges FSH and LH, accelerates follicle maturity and ovulation, and then improve the laying rate of laying hen;Corn flour is added, in corn flour Protein content is high, and amino acid rich in improves the laying rate and protein quality of laying hen;Add probiotics, Neng Gouwei Intestinal eubiosis is held, harmful bacteria growth is inhibited, and effectively decomposes the macromoleculars such as starch and protein in feed, improves and raises Expect utilization rate;Chinese herb feed additive is added, has the double action of pharmacological property and trophism concurrently, to the cholesterol for reducing laying hen egg Play the role of vital, and also improves the production performance of laying hen, Chinese herbal medicine not only amino acid rich in, dimension Raw element and microelement etc., the digestibility and utilization of promotion nutriment that can be direct or indirect, and contained alkaloid, polysaccharide With the bioactive substances such as organic acid, by its body itself to improve production performance, at the same toxic side effect is small, have no drug resistance, The advantages that not remaining;After wet basis fermentation raw material composition is made in wet basis fermentation raw material and other raw materials according to a certain ratio, no It only will not influence the content of the functional components such as the protein of script, probiotics, enzyme and organic acid in wet basis fermentation raw material, moreover it is possible to The degree that the water content control of entire wet basis fermentation raw material composition slowly, is chronically bred, grown in suitable probiotics, is protected It can also inhibit fungus growth while holding probiotic active, slow down the speed of egg feedstuff mildew, extend the storage of egg feedstuff Time limit.
Specific embodiment
Aiming at the problem that Egg Production of Laying Hens later period production performance, egg quality decline, by scientific matching, the present invention provides A kind of compound bacteria fermented feed and preparation method thereof.
Chinese herb feed additive has non-specific antibacterial action, fundamentally protects and coordinate the holistic health of animal, can be with Prevent disease, enhance the immune function of body, drive away internal harmful parasites, adjust internal beneficial microbe group, sufficiently sends out The potential ability having is waved and improves body itself, Chinese herb feed additive can be improved the digestibility of nutriment, utilization rate, increase The metabolism of strong livestock and poultry, promotes blood circulation, and improves the speed of growth.Chinese herbal medicine is many kinds of, resourceful, can take on the spot Material, and use simply, conveniently use easy to promote large-scale.Pure natural property Chinese herbal medicine is mostly real by medical treatment in thousands of years Trampling confirms natural plants or product useful and harmless to human and livestock health, can play the role of disease preventing and treating, enhancing development, Unique advantage is that drug residue free is used for a long time, without drug resistance and has no toxic side effect, and is that good Green medicament Chinese herbal medicine adds Add agent.Multiple functional Chinese herbal medicine is complicated organism, multicomponent itself is simultaneously subject to reasonable combination to form total tune unified Unique versatility can be fully adjusted the physiological function of body, have the double action of nutriment and drug concurrently, can both prevent and treat Disease can be improved production performance again, have nospecific immunity antibacterial action, and a certain single chemical component is difficult in Herbal medicine is mentioned in the same breath, be chemical synthetic drug it is incomparable.Chinese herbal feed additive is by various flavor function Herbal medicine is formulated according to Chinese veterinarian's theory and animal difference needs, since many kinds of, structure is complicated, ingredient multiplicity, is determined The Chinese herbal medicine of different compositions has different effect.
1 Chinese herb feed additive toxicity test of embodiment
Take the 28g root of Dahurain angelica, 22 g weeping forsythia leafs, 15 g caulis loniceraes, 20g ganoderma lucidum, 35g pueraria lobata and the 44 g fleece-flowers root;After mixing, It crushes, into smashed mixture plus the water of 1200g, decoction 3 hours are filtered, and the water that 1200g is added in filter residue decocts again 1.5 hours, filtering filtrate will mix twice, and be concentrated under reduced pressure into the decoction that crude drug concentration is 3g/ml.
ICR mouse is taken, weight is grouped at random at 25-30 grams, by weight, and every group 10, half male and half female, oral administration solution is given Medicine, dosage are 6.0ml/.It is observed continuously after administration 20 days, as a result all mouse are strong deposits, activity, diet, hair, row It is no abnormal to let out object, has no toxic reaction.Irritation test is carried out to white mouse oral cavity and damaged skin, has no irritant anti- It should be with other adverse reactions.
Toxicity test proves that the medicine no toxic effects and adverse reaction, clinical application are safe and reliable.
2 feed 1 of embodiment
The embodiment of the invention provides a kind of compound bacteria fermented feeds, wherein the parts by weight of each raw material in feed are as follows: corn flour 24 parts by weight, 34 parts by weight of wheat bran, 26 parts by weight of peanut meal, 12 parts by weight of seawood meal, 32 parts by weight of rice bran, wet basis proferment Expect 25 parts by weight, fermentation 10 parts by weight of adjuvant, 0.7 parts by weight of 0.35 parts by weight of antioxidant and yeast culture.
Preferably, the wet basis fermentation raw material is that wet basis fermentation cotton dregs, wet basis ferment rapeseed meal, wet basis fermented peanut meal, wet Any composition in base fermentation palm kernel meal and wet basis fermentation chrysanthemum meal;The wet basis fermentation raw material is made of following methods:
(1) prepared by fermentation substrate: taking wet basis fermentation cotton dregs, wet basis fermentation rapeseed meal, wet basis fermented peanut meal, wet basis fermentation palm kernel meal With wet basis fermentation chrysanthemum meal 75%~90%, water 20%~40%, mix to get fermentation substrate;
(2) prepared by mixed bacteria liquid: taking bacillus subtilis bacterium solution 1.5-3.2 parts by weight, bacillus licheniformis liquid 2.5-4.2 weight Number, lactobacillus suspension 1.2-4.0 parts by weight and the mixing of saccharomycete 1.5-3.2 parts by weight liquid are to get mixed bacteria liquid;
(3) mixed bacteria liquid obtained by step (2) is taken to be inoculated on fermentation substrate made from step (1), inoculum concentration is fermentation substrate weight The 3%~25% of amount mixes, and ferments 96 hours at 22~37 DEG C to get wet basis fermentation raw material;
Wherein, each number of live bacteria of probiotics in the mixed bacteria liquid are as follows: bacillus subtilis 3.6-12.8 × 108CFU/g, lichens bud Spore bacillus 1.3-4.5 × 108CFU/g, lactic acid bacteria 1.4-5.2 × 108CFU/g and saccharomycete 1.2-7.7 × 108CFU/g;
In the wet basis fermentation raw material, moisture be 38.0~60.0%, bacillus subtilis bacterial content be 2.5 × 109~6 × 109cfu/g, bacillus licheniformis content are 1.5 × 109~3 × 109cfu/g, the content of lactic acid bacteria is 2 × 109~5 × 109cfu/g, the content of saccharomycete are 2.2 × 109~3.5 × 109cfu/g, and total acid content is 4~10%.
Preferably, the fermentation adjuvant is the preparation method comprises the following steps: be added to 1500 milliliter of 30 0C's for fermenting 3 grams of adjuvant It in warm water, stirs, dissolution, after dissolution, places and activate within 2 hours spare, 40-70 grams of bacterium solution, urea after then taking activation 2-7 kilograms, 2-6 kilograms of salt, be put into 1200-2000 kilograms of cylinder or Chi Zhongjia water, stir evenly, then activated strain liquid It dissolves in water, fermentation adjuvant is made.
In order to preferably realize foregoing invention purpose, the present invention also provides a kind of preparation sides of compound bacteria fermented feed Method, wherein the preparation method includes:
(1) destruction step: weighing corn flour, wheat bran, peanut meal, seawood meal, rice bran by weight percentage, investment pulverizer into Row Crushing of Ultrafine, by smashing it through 2.0mm sieve;
(2) batching step: according to parts by weight than weighing fermentation adjuvant, antioxidant, yeast culture, with step (1) powder The fine powder of broken acquisition mixes, and puts into mixing machine, is uniformly mixed, incorporation time 20-30s;
(3) granulation step: investment granulator is pelletized, and pelleting temperature is 70 DEG C, its gelatinization degree is made to reach 90% or more;
(4) sieve classification classification step: was crushed after granulation again.
Wherein, in the step (4), the upper mesh size of the classification is 1.5-3.0mm, and lower mesh size is 1.0-2.0mm.
3 feed 2 of embodiment
The embodiment of the invention provides a kind of compound bacteria fermented feeds, wherein the parts by weight of each raw material in the feed are as follows: beautiful 35 parts by weight of rice flour, 22 parts by weight of wheat bran, 30 parts by weight of peanut meal, 14 parts by weight of seawood meal, 28 parts by weight of rice bran, wet basis hair 20 parts by weight of ferment raw material, fermentation 12 parts by weight of adjuvant, 0.4 parts by weight of antioxidant, 0.5 parts by weight of yeast culture and medium-height grass 0.32 parts by weight of medicine additive.
Wherein, in the Chinese herb feed additive each raw medicinal material parts by weight are as follows: root of Dahurain angelica 6-12 parts by weight, weeping forsythia leaf 4-8 parts by weight, caulis lonicerae 5-20 parts by weight, ganoderma lucidum 5-14 parts by weight, chamomile 10-25 parts by weight, pueraria lobata 8-16 parts by weight and Fleece-flower root 15-30 parts by weight.
In order to preferably realize foregoing invention purpose, the present invention also provides a kind of compound bacteria fermented feed, the medium-height grass Medicine additive is pulvis, application method are as follows: after the Chinese herb feed additive is processed into pulvis, with other feedstuffs or powder Other feedstuffs after broken are uniformly mixed, feeding after feeding or granulation.
In order to preferably realize foregoing invention purpose, the present invention also provides a kind of preparation sides of compound bacteria fermented feed Method, the Chinese herb feed additive are pulvis, preparation method are as follows:
The first step mixes the score ratio by weight of each raw medicinal material in the Chinese herb feed additive, and investment pulverizer carries out powder Broken or direct chopping is fitted into and decocts in container, and suitable quantity of water, which is added, makes it submerge medicinal material, after impregnating 30-50 minutes, is heated to boiling After 30-50 minutes, fried liquid is separated, the dregs of a decoction add water decoct 2-3 times again, respectively are isolated by fried liquid every time, merge institute The fried liquid having takes filtrate to abandon filter residue after filtering;
Second step, liquid cooling to be filtered but after, be added relative to 1-2 times of liquid weight after concentration determining alcohol be 80-90% ethyl alcohol, fill Divide and be uniformly mixed, stands overnight, obtain filtrate after so that its precipitating, next day is taken its supernatant liquid filtering, sediment is 50- with determining alcohol 60% ethyl alcohol is cleaned, and is collected washing lotion and is merged with filtrate, after ethyl alcohol is withdrawn in decompression, stands 0.5-1 hours, makes precipitating completely, mistake Filter, filtrate low-temperature evaporation obtain paste;
Step 3: paste placed into spray dryer it is dry, guarantee inlet air temperature at 180 DEG C -200 DEG C, leaving air temp 70 DEG C -90 DEG C, it is then ground into -300 mesh of 200 mesh, obtains pulvis.
Preferably, the wet basis fermentation raw material is that wet basis fermentation cotton dregs, wet basis ferment rapeseed meal, wet basis fermented peanut meal, wet Any composition in base fermentation palm kernel meal and wet basis fermentation chrysanthemum meal;The wet basis fermentation raw material is made of following methods:
(1) prepared by fermentation substrate: taking wet basis fermentation cotton dregs, wet basis fermentation rapeseed meal, wet basis fermented peanut meal, wet basis fermentation palm kernel meal With wet basis fermentation chrysanthemum meal 75%~90%, water 20%~40%, mix to get fermentation substrate;
(2) prepared by mixed bacteria liquid: taking bacillus subtilis bacterium solution 1.5-3.2 parts by weight, bacillus licheniformis liquid 2.5-4.2 weight Number, lactobacillus suspension 1.2-4.0 parts by weight and the mixing of saccharomycete 1.5-3.2 parts by weight liquid are to get mixed bacteria liquid;
(3) mixed bacteria liquid obtained by step (2) is taken to be inoculated on fermentation substrate made from step (1), inoculum concentration is fermentation substrate weight The 3%~25% of amount mixes, and ferments 96 hours at 22~37 DEG C to get wet basis fermentation raw material;
Wherein, each number of live bacteria of probiotics in the mixed bacteria liquid are as follows: bacillus subtilis 3.6-12.8 × 108CFU/g, lichens bud Spore bacillus 1.3-4.5 × 108CFU/g, lactic acid bacteria 1.4-5.2 × 108CFU/g and saccharomycete 1.2-7.7 × 108CFU/g;
In the wet basis fermentation raw material, moisture be 38.0~60.0%, bacillus subtilis bacterial content be 2.5 × 109~6 × 109cfu/g, bacillus licheniformis content are 1.5 × 109~3 × 109cfu/g, the content of lactic acid bacteria is 2 × 109~5 × 109cfu/g, the content of saccharomycete are 2.2 × 109~3.5 × 109cfu/g, and total acid content is 4~10%.
Preferably, the fermentation adjuvant is the preparation method comprises the following steps: be added to 1500 milliliter of 30 0C's for fermenting 3 grams of adjuvant It in warm water, stirs, dissolution, after dissolution, places and activate within 2 hours spare, 40-70 grams of bacterium solution, urea after then taking activation 2-7 kilograms, 2-6 kilograms of salt, be put into 1200-2000 kilograms of cylinder or Chi Zhongjia water, stir evenly, then activated strain liquid It dissolves in water, fermentation adjuvant is made.
In order to preferably realize foregoing invention purpose, the present invention also provides a kind of preparation sides of compound bacteria fermented feed Method, wherein the preparation method includes:
(1) destruction step: weighing corn flour, wheat bran, peanut meal, seawood meal, rice bran by weight percentage, investment pulverizer into Row Crushing of Ultrafine, by smashing it through 2.0mm sieve;
(2) batching step: according to parts by weight than weighing fermentation adjuvant, antioxidant, yeast culture and Chinese herbal medicine addition Agent crushes the fine powder obtained with step (1) and mixes, put into mixing machine, is uniformly mixed, incorporation time 20-30s;
(3) granulation step: investment granulator is pelletized, and pelleting temperature is 70 DEG C, its gelatinization degree is made to reach 90% or more;
(4) sieve classification classification step: was crushed after granulation again.
Wherein, in the step (4), the upper mesh size of the classification is 1.5-3.0mm, and lower mesh size is 1.0-2.0mm.
Comparative test
Sample plot point chooses Qingdao six and laying hen chicken house, and company technique portion carries out 60 days by a definite date in spring in 2018 in laying hen Feeding experiment.1800 normal laying hens of 42 week old are chosen in test, are divided into three experimental groups immediately, respectively using embodiment 2 Feed 1, the feed 2 of embodiment 3 and traditional egg feedstuff are fed, during test, count each experimental group Egg Production of Laying Hens quantity and Egg size, and laying rate of laying hen and average egg weight are calculated, and the quality and cholesterol level for egg of making a random inspection immediately, as a result such as Shown in table 1, table 2 and table 3.
The table of comparisons of 1 laying rate of laying hen of table and average egg weight
Project Embodiment group 1 Embodiment group 2 Control group
Laying rate (%) 95.72 98.95 80.72
Average egg weight (g) 69.352 70.521 66.753
It was found from the data of table 1: compared with the laying hen that conventional feed is fed, using the laying hen of Example 1 and Example 2 of the present invention The laying hen of forage feed, laying rate are higher by 18% or so, and average egg weight is higher by 3.2g or so.
The 2 egg quality table of comparisons of table
Project Embodiment group 1 Embodiment group 2 Control group
Egg shape index 1.375 1.401 1.339
Eggshell strength (MPa) 4.32 4.40 3.45
Shell thickness (mm) 0.375 0.360 0.331
Yolk color (grade) 7.91 7.94 6.1
Albumen height (mm) 5.698 5.893 5.646
It was found from the data of table 2: using Example 1 and Example 2 of the present invention egg feedstuff feed laying hen, egg it is each The item index of quality is above the laying hen egg of conventional feed nursing.
The 3 Egg cholesterol amount table of comparisons of table
Project Embodiment group 1 Embodiment group 2 Control group
Cholesterol (mg/) 116.93 114.23 219.89
It was found from the data of table 3: the laying hen fed using the egg feedstuff of Example 1 and Example 2 of the present invention, the gallbladder of egg Sterol content is significantly lower than the laying hen egg that conventional feed is fed.
By comparative experiments it is found that the laying hen that the egg feedstuff that the present invention matches is fed not only increases laying rate and egg Quality, and significantly reduce the content of cholesterol in egg, mixed feed of the invention can be good at meeting laying hen production The nutritional need of egg phase.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.

Claims (10)

1. a kind of compound bacteria fermented feed, which is characterized in that the feed includes following raw material: corn flour, wheat bran, peanut The dregs of rice, seawood meal, rice bran, wet basis fermentation raw material, fermentation adjuvant, antioxidant and yeast culture.
2. compound bacteria fermented feed according to claim 1, which is characterized in that the parts by weight of each raw material in the feed Are as follows: corn flour 20-40 parts by weight, wheat bran 12-24 parts by weight, peanut meal 10-32 parts by weight, seawood meal 7-18 parts by weight, rice Chaff 12-35 parts by weight, wet basis fermentation raw material 14-28 parts by weight, fermentation adjuvant 8-26 parts by weight, antioxidant 0.3-0.5 weight Measure part and yeast culture 0.2-1.0 parts by weight.
3. compound bacteria fermented feed according to claim 1 to 3, which is characterized in that further include medium-height grass in the feed Medicine additive, parts by weight are as follows: corn flour 20-40 parts by weight, wheat bran 12-24 parts by weight, peanut meal 10-32 parts by weight, Seawood meal 7-18 parts by weight, rice bran 12-35 parts by weight, wet basis fermentation raw material 14-28 parts by weight, fermentation adjuvant 8-26 weight Part, antioxidant 0.3-0.5 parts by weight, yeast culture 0.2-1.0 parts by weight and Chinese herb feed additive 0.32-0.48 weight Part.
4. compound bacteria fermented feed according to claim 1 to 4, which is characterized in that the weight of each raw material in the feed Measure number are as follows: 35 parts by weight of corn flour, 22 parts by weight of wheat bran, 30 parts by weight of peanut meal, 14 parts by weight of seawood meal, 28 weight of rice bran Measure part, 20 parts by weight of wet basis fermentation raw material, fermentation 12 parts by weight of adjuvant, 0.4 parts by weight of antioxidant, yeast culture 0.5 0.32 parts by weight of parts by weight and Chinese herb feed additive.
5. compound bacteria fermented feed according to claim 1 to 4, which is characterized in that each in the Chinese herb feed additive The parts by weight of raw medicinal material are as follows: root of Dahurain angelica 6-12 parts by weight, weeping forsythia leaf 4-8 parts by weight, caulis lonicerae 5-20 parts by weight, ganoderma lucidum 5- 14 parts by weight, chamomile 10-25 parts by weight, pueraria lobata 8-16 parts by weight and fleece-flower root 15-30 parts by weight.
6. compound bacteria fermented feed according to claim 1 to 4, which is characterized in that the Chinese herb feed additive is powder Agent, application method are as follows: after the Chinese herb feed additive is processed into pulvis, with other feedstuffs or other smashed feedings Expect that raw material is uniformly mixed, feeding after feeding or granulation.
7. the preparation method of -6 any compound bacteria fermented feeds according to claim 1, which is characterized in that the Chinese herbal medicine Additive is pulvis, preparation method are as follows:
The first step mixes the score ratio by weight of each raw medicinal material in the Chinese herb feed additive, and investment pulverizer carries out powder Broken or direct chopping is fitted into and decocts in container, and suitable quantity of water, which is added, makes it submerge medicinal material, after impregnating 30-50 minutes, is heated to boiling After 30-50 minutes, fried liquid is separated, the dregs of a decoction add water decoct 2-3 times again, respectively are isolated by fried liquid every time, merge institute The fried liquid having takes filtrate to abandon filter residue after filtering;
Second step, liquid cooling to be filtered but after, be added relative to 1-2 times of liquid weight after concentration determining alcohol be 80-90% ethyl alcohol, fill Divide and be uniformly mixed, stands overnight, obtain filtrate after so that its precipitating, next day is taken its supernatant liquid filtering, sediment is 50- with determining alcohol 60% ethyl alcohol is cleaned, and is collected washing lotion and is merged with filtrate, after ethyl alcohol is withdrawn in decompression, stands 0.5-1 hours, makes precipitating completely, mistake Filter, filtrate low-temperature evaporation obtain paste;
Step 3: paste placed into spray dryer it is dry, guarantee inlet air temperature at 180 DEG C -200 DEG C, leaving air temp 70 DEG C -90 DEG C, it is then ground into -300 mesh of 200 mesh, obtains pulvis.
8. compound bacteria fermented feed according to claim 1-7, which is characterized in that the wet basis fermentation raw material is It is any in wet basis fermentation cotton dregs, wet basis fermentation rapeseed meal, wet basis fermented peanut meal, wet basis fermentation palm kernel meal and wet basis fermentation chrysanthemum meal The composition of kind;The wet basis fermentation raw material is made of following methods:
(1) prepared by fermentation substrate: taking wet basis fermentation cotton dregs, wet basis fermentation rapeseed meal, wet basis fermented peanut meal, wet basis fermentation palm kernel meal With wet basis fermentation chrysanthemum meal 75%~90%, water 20%~40%, mix to get fermentation substrate;
(2) prepared by mixed bacteria liquid: taking bacillus subtilis bacterium solution 1.5-3.2 parts by weight, bacillus licheniformis liquid 2.5-4.2 weight Number, lactobacillus suspension 1.2-4.0 parts by weight and the mixing of saccharomycete 1.5-3.2 parts by weight liquid are to get mixed bacteria liquid;
(3) mixed bacteria liquid obtained by step (2) is taken to be inoculated on fermentation substrate made from step (1), inoculum concentration is fermentation substrate weight The 3%~25% of amount mixes, and ferments 96 hours at 22~37 DEG C to get wet basis fermentation raw material;
Wherein, each number of live bacteria of probiotics in the mixed bacteria liquid are as follows: bacillus subtilis 3.6-12.8 × 108CFU/g, lichens bud Spore bacillus 1.3-4.5 × 108CFU/g, lactic acid bacteria 1.4-5.2 × 108CFU/g and saccharomycete 1.2-7.7 × 108CFU/g;
In the wet basis fermentation raw material, moisture is 38.0~60.0%, and bacillus subtilis bacterial content is 2.5 × 109~6 × 109Cfu/g, bacillus licheniformis content are 1.5 × 109~3 × 109Cfu/g, the content of lactic acid bacteria are 2 × 109~5 × 109Cfu/g, the content of saccharomycete are 2.2 × 109~3.5 × 109Cfu/g, total acid content are 4~10%.
9. compound bacteria fermented feed according to claim 1-8, which is characterized in that the fermentation adjuvant preparation Method are as follows: be added in the warm water of 1500 milliliter of 30 0C, stir by fermenting 3 grams of adjuvant, dissolution after dissolution, is placed 2 hours Activate it is spare, then take activation after 40-70 grams of bacterium solution, 2-7 kilograms of urea, 2-6 kilograms of salt, be put into cylinder or Chi Zhongjia It 1200-2000 kilograms of water, stirs evenly, then activated strain liquid is dissolved in water, fermentation adjuvant is made.
10. a kind of preparation method of such as described in any item compound bacteria fermented feeds of claim 1-9, which is characterized in that described Preparation method includes:
(1) destruction step: weighing corn flour, wheat bran, peanut meal, seawood meal, rice bran by weight percentage, investment pulverizer into Row Crushing of Ultrafine, by smashing it through 2.0mm sieve;
(2) batching step: according to parts by weight than weighing fermentation adjuvant, antioxidant, yeast culture and Chinese herbal medicine addition Agent crushes the fine powder obtained with step (1) and mixes, put into mixing machine, is uniformly mixed, incorporation time 20-30s;
(3) granulation step: investment granulator is pelletized, and pelleting temperature is 70 DEG C, its gelatinization degree is made to reach 90% or more;
(4) sieve classification classification step: was crushed after granulation again.
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