CN103875982A - Preparation method for constipation-resisting fermented feed - Google Patents

Preparation method for constipation-resisting fermented feed Download PDF

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Publication number
CN103875982A
CN103875982A CN201410129836.1A CN201410129836A CN103875982A CN 103875982 A CN103875982 A CN 103875982A CN 201410129836 A CN201410129836 A CN 201410129836A CN 103875982 A CN103875982 A CN 103875982A
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constipation
fermented feed
preparation
fermentation
resisting
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CN103875982B (en
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程茂基
程勐万里
孟龙
薛芹
吕锋
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SHAANXI SHIYANG AGRICULTURE Co.,Ltd.
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ANHUI WULIANGTAI BIOLOGICAL ENGINEERING Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention belongs to the technical field of feeds, enzyme preparations and microorganisms and particularly relates to a preparation method for a constipation-resisting fermented feed. The preparation method comprises the following steps: mixing and blending soya bean meal, oats, beet pulp and water to prepare a fermented material wet base; adding saccharomycetes strain liquid, lactic acid bacteria strain liquid and microorganism protease into the fermented material wet base; carrying out solid-state anaerobic fermentation and enzymolysis; and drying to prepare the constipation-resisting fermented feed. The constipation-resisting fermented feed prepared by the preparation method is enriched with micro-molecular nutrition including soluble fibers, small peptides, lactic acids, yeast proteins, probiotics and the like, and has the characteristics of constipation resistance, good palatability, easiness for digestion, low content of anti-nutritional factors and the like.

Description

A kind of preparation method of anti-constipation fermented feed
Technical field
The invention belongs to feed, enzyme preparation and microbial technology field, be specifically related to a kind of preparation method of anti-constipation fermented feed.
Background technology
China is populous nation, Ye Shi pig industry big country.2012, Chinese Pigs meat production reached 5,335 ten thousand tons, and live pig is delivered 6.96 hundred million for sale, wherein 5,200 ten thousand of sows.Be accompanied by the development of pig industry, pig feed rate of industrial development is surprising.According to Ministry of Agriculture's statistics, 7,600 ten thousand tons of Chinese pig feed output in 2012, wherein, sow feed is up to 5,000,000 tons.
In sow breeding production, constipation has a strong impact on sow reproductive performance.Sow generation constipation, the lighter can cause sow poor appetite, lassitude, nutrition insufficiency of intake, cause stillborn foetus, miscarriage, difficult labour and postpartum agalasisa disease etc.Severe one can cause sow to digest and assimilate disorder, insanity, and appetite is absolutely useless, does not rise sleepingly, occasionally has heating.The sow physique that the course of disease is long is weaker, and last physique seriously dies of exhaustion and dies.In recent years, how to prevent or slow down prevention of sow constipation and receive much concern.
Beet pulp is the accessory substance after beet sugar manufacture, contains 93% moisture, the dry of 7% left and right.In dry, containing abundant each amino acid, vitamin and trace element, and contain promotion milk cattle rumen fermentation and healthy betaine, pectin and nicotinic acid isoreactivity composition, is the desirable feed of sow, milk cow.Research is found: in sow diet in postpartum, add beet and can prevent the generation of sow apositia in postpartum and constipation.
In soybean, be rich in soyabean oligosaccharides (Soybean Oligosaccharide), it extracts separation from soybean, is not comprise monose, is the mixture of master's small molecular sugar by disaccharides more than monose, trisaccharide, tetrose.Conventionally soyabean oligosaccharides detects main two of stachyose, the gossyposes that detect.
Soyabean oligosaccharides possesses vicidity, retentiveness and the water-swollen physical characteristic of part dietary fiber.Soybean by fermentation after, compound sugar increased activity, can sting sow and swash intestines peristalsis, increase sow ight soil wettability, keep certain osmotic pressure, promotes defecation, thereby alleviates or prevent the generation of prevention of sow constipation.Research shows: in sow feed, add 2000~3000 gs/kg of compound sugar, can effectively prevent prevention of sow constipation.
Oat reaches 15.6% containing thick protein, and fat 8.5% also has the elements such as starch release heat and phosphorus, iron, calcium, is unique crop of closing soap formula element in cereal, and it can regulate the functions of intestines and stomach of animal, reduces anus sterol.In oat, richness is closed two kinds of important dietary fibers: Soluble Fiber and non-solubility fiber, and wherein Soluble Fiber can promote intestines peristalsis, and non-solubility fiber contributes to digestion, and the two all can Constipation.
Fermented feed is the good feed processing and treating method of sow.Feed is through the not only degradable ANFs that ferments, but also can produce small molecule bioactive material, as little peptide, lactic acid, vitamin, probio and unknown somatomedin etc., but also can promote lactation, be the best substitute of fish meal, PURE WHEY.
Therefore, the present invention intends dregs of beans, oat, beet pulp to ferment, the anti-constipation fermented feed of Development and Production.
Summary of the invention
The present invention is intended to prevent prevention of sow constipation, promotes the functions such as lactation, a kind of preparation method of anti-constipation fermented feed is provided, anti-constipation fermented feed prepared by the method is rich in the little molecular nutritions such as Soluble Fiber, little peptide, lactic acid, Yeast protein and probio, has the features such as anti-constipation, good palatability, easy to digest and ANFs content is low.
The technical solution adopted in the present invention is: the preparation method of anti-constipation fermented feed, and step is as follows:
1), dregs of beans, oat, beet pulp and water are mixed modified, make fermentation material wet basis;
2), in fermentation material wet basis, add saccharomycete strain liquid, lactic acid bacteria culturers liquid and microbial protease;
3), solid anaerobic digestion enzymolysis;
4), dry, make anti-constipation fermented feed.
Further, step 1) in the weight ratio of water, dregs of beans, oat and beet pulp be 100: 1~200: 2~500: 2~500.
Preferably, step 1) in the weight ratio of water, dregs of beans, oat and beet pulp be 100: 50~100: 80~200: 80~200.
Further, step 2) in the weight ratio of fermentation material wet basis, saccharomycete strain liquid, lactic acid bacteria culturers liquid be 100: 0.5~20: 0.5~20, the addition of microbial protease is 0.01~1000U/g substrate.
Preferably, step 2) in the weight ratio of fermentation material wet basis, saccharomycete strain liquid, lactic acid bacteria culturers liquid and microbial protease be 100: 2~8: 3~15, the addition of microbial protease is 100~300U/g substrate.
Further, step 3) in fermentation, hydrolysis temperature be 20~60 DEG C, fermentation, enzymolysis time are 8~240h, fermentation Initial pH is 4~8.
Preferably, step 3) in fermentation, hydrolysis temperature be 28~42 DEG C, fermentation, enzymolysis time are 36~72h, fermentation Initial pH is 5.8~6.2.
Anti-constipation fermented feed prepared by the present invention, is rich in the little molecular nutritions such as Soluble Fiber, little peptide, lactic acid, Yeast protein and probio, has the features such as anti-constipation, good palatability, easy to digest and ANFs content is low.
Detailed description of the invention
Below with reference to embodiment, the present invention is described in detail.
Embodiment 1
20kg dregs of beans, 40kg oat, 40kg beet pulp and 35kg water are mixed in proportion modified, make fermentation material wet basis.In fermentation material wet basis, add 4kg saccharomycete strain liquid, 6kg lactic acid bacteria culturers liquid and 1.2kg microbial protease (200U/g substrate wet basis), be under 6.0 conditions at 30 DEG C with pH, after fermentation, enzymolysis time 48h, dry and make the anti-constipation fermented feed of 98kg.
Embodiment 2
30kg dregs of beans, 30kg oat, 40kg beet pulp and 40kg water are mixed in proportion modified, make fermentation material wet basis.In fermentation material wet basis, add 3kg saccharomycete strain liquid, 5kg lactic acid bacteria culturers liquid and 1.0kg microbial protease (100U/g substrate wet basis), be under 6.2 conditions at 34 DEG C with pH, after fermentation, enzymolysis time 72h, dry and make the anti-constipation fermented feed of 97kg.
Embodiment 3
100kg dregs of beans, 500kg oat, 40kg beet pulp and 350kg water are mixed in proportion modified, make fermentation material wet basis.In fermentation material wet basis, add 50kg saccharomycete strain liquid, 80kg lactic acid bacteria culturers liquid and 10kg microbial protease (300U/g substrate wet basis), be under 5.8 conditions at 36 DEG C with pH, after fermentation, enzymolysis time 60h, dry and make the anti-constipation fermented feed of 960kg.
Embodiment 4
40kg dregs of beans, 20kg oat, 40kg beet pulp and 40kg water are mixed in proportion modified, make fermentation material wet basis.In fermentation material wet basis, add 5kg saccharomycete strain liquid, 8kg lactic acid bacteria culturers liquid and 1.5kg microbial protease (200U/g substrate wet basis), be under 6.0 conditions at 38 DEG C with pH, after fermentation, enzymolysis time 48h, dry and make the anti-constipation fermented feed of 98kg.
Embodiment 5
10kg dregs of beans, 20kg oat, 70kg beet pulp and 35kg water are mixed in proportion modified, make fermentation material wet basis.In fermentation material wet basis, add 5kg saccharomycete strain liquid, 5kg lactic acid bacteria culturers liquid and 0.8kg microbial protease (200U/g substrate wet basis), be under 6.2 conditions at 42 DEG C with pH, after fermentation, enzymolysis time 60h, dry and make the anti-constipation fermented feed of 95kg.
The feeding effect of the anti-constipation fermented feed that, prepared by embodiment 1
Choose body condition, parity, body weight and the expected date of childbirth 160 of close healthy Du Luoke milking sows in six Anxi quotient sets group one branches, commodity pig farm, Anhui, be divided at random 4 groups, every group of 5 repetitions, 8 sows of each repetition, to study the actual feeding effect of five grain health (anti-constipation fermented feed), i.e. control group, compound sugar group, 10% 5 grain health group, 20% 5 grain health group.Test finishes in the time that gestation starts to piglet wean in 21 days for 108 days.
4 groups of test sucking pig basal diet compositions are all identical with nutrition.Contrast A group is neither added compound sugar, does not also add five grain health; Contrast B group is added 3% compound sugar, but does not add five grain health; 10% 5 grain health group is added 10% 5 grain health; 20% 5 grain health group is added 20% 5 grain health.Test daily ration composition refers to table 1 with trophic level, and five grain health refer to table 2 to the impact of milking sow production performance.
Test sow house is roof double-pitch type, cement flooring, and ventilation and lighting is better, natural lighting on daytime, ventilation, night lights illumination, normal immunity and management.Pig house temperature controls to and is not less than 15 DEG C, and humidity is controlled at 55~65%.Accomplish that environmental condition is consistent, with at a draft supporting member's management, free choice feeding and drinking-water as far as possible.
Table 1 is tested daily ration composition and trophic level
Figure BSA0000102574020000051
Note: 4% premix contains calcium formate, calcium monohydrogen phosphate, salt, lysine, methionine, vitamin, trace element and medicine etc.
Table 2 five impacts of grain health on milking sow production performance
Known by table 2, in lactating swine diets, use compound sugar, 10% 5 grain health and 20% 5 grain health to feed behind 27-~28 day, the daily gain of piglet improves 4.11%, 13.72% and 16.30% (p < 0.05 or p < 0.01) than control group respectively.The constipation rate of milking sow has reduced by 71.42%, 92.85%, 100%.Result shows: five grain health can obviously improve the speed of growth of piglet, significantly reduce prevention of sow constipation.
Two, main active substances content in the anti-constipation fermented feed that prepared by embodiment 1, in table 3.
Main active substances content in the anti-constipation fermented feed of table 3
Project Index Project Index
Color and luster Yellowish-brown or light yellow Lactic acid/% ≥1
Moisture/% ≤10.0 Crude fibre/% ≥8.0
Thick protein/% ≥12.0 Coarse ash/% ≤0.5
Crude fat/% ≥2.0 Lysine/% ≥0.45
Little peptide albumen/% ≥2 Methionine/% ≥0.15
Above content is only made for the present invention giving an example and explanation; affiliated those skilled in the art make various amendments to described specific embodiment or supplement or adopt similar mode to substitute; only otherwise depart from the design of invention or surmount this scope as defined in the claims, all should belong to protection scope of the present invention.

Claims (7)

1. a preparation method for anti-constipation fermented feed, is characterized in that, step is as follows:
1), dregs of beans, oat, beet pulp and water are mixed modified, make fermentation material wet basis;
2), in fermentation material wet basis, add saccharomycete strain liquid, lactic acid bacteria culturers liquid and microbial protease;
3), solid anaerobic digestion enzymolysis;
4), dry, make anti-constipation fermented feed.
2. the preparation method of anti-constipation fermented feed according to claim 1, is characterized in that step 1) in the weight ratio of water, dregs of beans, oat and beet pulp be 100: 1~200: 2~500: 2~500.
3. the preparation method of anti-constipation fermented feed according to claim 2, is characterized in that step 1) in the weight ratio of water, dregs of beans, oat and beet pulp be 100: 50~100: 80~200: 80~200.
4. the preparation method of anti-constipation fermented feed according to claim 2, it is characterized in that, step 2) in the weight ratio of fermentation material wet basis, saccharomycete strain liquid, lactic acid bacteria culturers liquid be 100: 0.5~20: 0.5~20, the addition of microbial protease is 0.01~1000U/g substrate.
5. the preparation method of anti-constipation fermented feed according to claim 4, it is characterized in that, step 2) in the weight ratio of fermentation material wet basis, saccharomycete strain liquid, lactic acid bacteria culturers liquid and microbial protease be 100: 2~8: 3~15, the addition of microbial protease is 100~300U/g substrate.
6. the preparation method of anti-constipation fermented feed according to claim 4, is characterized in that step 3) in fermentation, hydrolysis temperature is 20~60 DEG C, fermentation, enzymolysis time are 8~240h, fermentation Initial pH is 4~8.
7. the preparation method of anti-constipation fermented feed according to claim 6, is characterized in that step 3) in fermentation, hydrolysis temperature is 28~42 DEG C, fermentation, enzymolysis time are 36~72h, fermentation Initial pH is 5.8~6.2.
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Cited By (11)

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CN103892136A (en) * 2014-04-16 2014-07-02 安徽广通生物科技有限责任公司 Fermented feed for resisting sow constipation and preparation method of fermented feed
CN104171494A (en) * 2014-07-02 2014-12-03 中粮营养健康研究院有限公司 Feed capable of improving the productive performance of lactating sows and feeding method thereof
CN104171556A (en) * 2014-07-10 2014-12-03 南宁荣港生物科技有限公司 Novel biological paste fodder for weaned pigs and preparation method thereof
CN104206692A (en) * 2014-09-19 2014-12-17 龙岩市益发畜牧发展有限公司 Preparation method of attractant anti-constipation fermented feed
CN108497187A (en) * 2018-03-28 2018-09-07 周口广安农牧科技有限公司 A kind of yellow serofluid promotees the preparation method of newborn glycolysis dregs of beans
CN108634112A (en) * 2018-03-30 2018-10-12 山东丰沃新农农牧科技有限公司 A kind of dietary fiber type religion guarantor's material
CN108835420A (en) * 2018-07-25 2018-11-20 日照普惠动物营养科技有限公司 A kind of dedicated anaerobic type biological fermentation feed of pregnant sow and preparation method
CN109170275A (en) * 2018-10-19 2019-01-11 北京嘉博文生物科技有限公司 Fermented feed and preparation method thereof and the application in improvement constipation
CN109805204A (en) * 2019-01-28 2019-05-28 山东新希望六和集团有限公司 A kind of compound bacteria fermented feed and preparation method thereof
WO2019122192A1 (en) * 2017-12-22 2019-06-27 Hamlet Protein A/S Vertical plug-flow process for bio-conversion of biomass involving enzymes
CN111480742A (en) * 2020-04-26 2020-08-04 湖南普菲克生物科技有限公司 Fermented feed for improving constipation of sows and preparation method thereof

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103892136A (en) * 2014-04-16 2014-07-02 安徽广通生物科技有限责任公司 Fermented feed for resisting sow constipation and preparation method of fermented feed
CN104171494A (en) * 2014-07-02 2014-12-03 中粮营养健康研究院有限公司 Feed capable of improving the productive performance of lactating sows and feeding method thereof
CN104171556A (en) * 2014-07-10 2014-12-03 南宁荣港生物科技有限公司 Novel biological paste fodder for weaned pigs and preparation method thereof
CN104206692A (en) * 2014-09-19 2014-12-17 龙岩市益发畜牧发展有限公司 Preparation method of attractant anti-constipation fermented feed
WO2019122192A1 (en) * 2017-12-22 2019-06-27 Hamlet Protein A/S Vertical plug-flow process for bio-conversion of biomass involving enzymes
CN111479472A (en) * 2017-12-22 2020-07-31 哈姆雷特蛋白质有限公司 Vertical plug flow process for bioconversion of biomass involving enzymes
CN108497187A (en) * 2018-03-28 2018-09-07 周口广安农牧科技有限公司 A kind of yellow serofluid promotees the preparation method of newborn glycolysis dregs of beans
CN108634112A (en) * 2018-03-30 2018-10-12 山东丰沃新农农牧科技有限公司 A kind of dietary fiber type religion guarantor's material
CN108835420A (en) * 2018-07-25 2018-11-20 日照普惠动物营养科技有限公司 A kind of dedicated anaerobic type biological fermentation feed of pregnant sow and preparation method
CN109170275A (en) * 2018-10-19 2019-01-11 北京嘉博文生物科技有限公司 Fermented feed and preparation method thereof and the application in improvement constipation
CN109805204A (en) * 2019-01-28 2019-05-28 山东新希望六和集团有限公司 A kind of compound bacteria fermented feed and preparation method thereof
CN111480742A (en) * 2020-04-26 2020-08-04 湖南普菲克生物科技有限公司 Fermented feed for improving constipation of sows and preparation method thereof

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Inventor after: Cheng Maoji

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