CN104041710A - Preparation method of fish meal small peptide feed - Google Patents
Preparation method of fish meal small peptide feed Download PDFInfo
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- CN104041710A CN104041710A CN201410279916.5A CN201410279916A CN104041710A CN 104041710 A CN104041710 A CN 104041710A CN 201410279916 A CN201410279916 A CN 201410279916A CN 104041710 A CN104041710 A CN 104041710A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention belongs to the technical field of fermentation of feeds, enzymic preparations and microorganisms and in particular relates to a preparation method of a fish meal small peptide feed. The preparation method of the fish meal small peptide feed comprises the following steps: mixing commercially-available poor-quality fish meal, molasses, brown sugar or glucose, water, microorganism protease, lactic acid bacteria strain liquid and saccharomycetes strain liquid to prepare a liquid fermentation culture medium, carrying out primary liquid fermentation and enzymolysis for 2-120 hours at the temperature being 20-70 DEG C to prepare fish meal small peptide liquid; mixing the fish meal small peptide liquid, corn flour and alfalfa powder to prepare a solid fermentation culture medium, carrying out secondary solid fermentation and enzymolysis for 6-360 hours at the temperature being 10-50 DEG C to prepare the fish meal small peptide feed. The prepared fish meal small peptide feed is capable of removing anti-nutritional factors, killing pathogenic microorganisms, producing abundant small peptides, lactic acid, yeast protein and probiotics, removing odor, presenting fishy sourness, improving the feed intake of livestock and poultry, promoting growth of piglets and improving milk yield of milking sows.
Description
Technical field
The invention belongs to feed, enzyme preparation and microbial fermentation technology field, specifically relate to a kind of preparation method of fish meal small peptide feed.
Background technology
China is populous nation, is also the first in the world big country of raising pigs.2011, the Animal Husbandry in China output value reached 2.2 trillion yuan, and live pig is delivered 6.46 hundred million for sale, and wherein sow is 4,800 ten thousand, 5,053 ten thousand tons of pork output.According to Ministry of Agriculture's statistics, within 2011, Chinese commodity feed total output will reach 1.80 hundred million tons, and wherein mixed feed is 1.49 hundred million tons; 2,500 ten thousand tons of concentrated food stuffs; 6,050,000 tons of additive premixes, wherein sow feed is more than 500 ten thousand tons.Within 2011, the national feed industry gross output value is 5,410 hundred million yuan.Expect national mixed feed output in 2015 and will reach more than 2.0 hundred million tons, wherein sow feed will reach more than more than 800 ten thousand tons.
As everyone knows, fish meal is the animal fodder raw material that livestock and poultry effect is the most definite and stable.Animal and fowl fodder adds 3～5% high-quality fish meal, and the speed of growth improves more than 6%, adds 3% fish meal in sow feed, and lactation amount improves more than 8%.2013, whole world fish meal output reached 5,300,000 tons, and wherein Chinese domestic fish meal output reaches more than 30 ten thousand tons.China is fish meal import and consumption big country, and within 2013, Chinese imported fish meal is more than 120 ten thousand tons.
Fish meal is animal fodder raw material, and its nutrition is very abundant, especially easily by pathogenic microorganisms such as Escherichia coli, salmonellas, is polluted, and has produced a large amount of toxin such as histamine, cadaverine.According to test, current commercially available domestic fish meal is fish meal inferior mostly, ubiquity quality is not high, the toxin such as the pathogenic microorganism such as Escherichia coli, salmonella and histamine, cadaverine are severe overweight often, what have even exceeds standard hundreds of times, present strong stink, cause feeding effect to decline, even can be not feeding.Therefore, how to improve commercially available fish meal quality inferior, the content of toxins such as pathogenic microbe killing and reduction histamine, cadaverine are extremely urgent problems.
Microorganism fermentation is one of behave of killing feed pathogenic microorganism and toxin.Lactic acid bacteria, by secretion lactic acid, reduces pH and kills feed pathogenic microorganism, and saccharomycete preferentially utilizes nonprotein nitrogen (NPN), can preferentially consume the nonprotein nitrogen such as histamine, cadaverine, significantly reduces the content of toxins such as histamine, cadaverine.
Summary of the invention
It is serious that the present invention is intended to overcome the commercially available fish meal microbiological contamination inferior of above-mentioned China, the difficult problems such as the content of toxins such as histamine, cadaverine exceeds standard, provide a kind of preparation method up to standard to prepare the content of toxins such as pathogenic microorganism and histamine, cadaverine, be rich in little peptide, lactic acid, Yeast protein and probio, have that nutrition is high, easy to digest, good palatability, obviously improve livestock and poultry feed intake, promote piglet growth, improve the fish meal small peptide feed of milking sow lactation amount.
In order to solve the problems of the technologies described above, the technical scheme adopting is: a kind of preparation method of fish meal small peptide feed, and step is as follows:
1., a liquid fermentation enzymolysis is prepared the little peptide liquid of fish meal protein
Commercially available fish meal inferior, molasses, brown sugar or glucose, water, microbial protease, lactic acid bacteria culturers liquid and saccharomycete strain liquid hybrid modulation are become to liquid fermentation medium, liquid fermentation enzymolysis 2～120h once in 20～70 ℃ of conditions, makes the little peptide liquid of fish meal protein;
In liquid fermentation medium, the weight ratio of commercially available fish meal inferior, molasses, brown sugar or glucose, water, lactic acid bacteria culturers liquid and saccharomycete strain liquid is 100:1～500:0.1～100:100～1000:1～20:1～20, and in liquid fermentation medium, the addition of microbial protease is 0.005～2000U/g substrate wet basis;
2., the little peptide liquid of fish meal protein secondary solid state fermentation enzymolysis is prepared fish meal small peptide feed
The little peptide liquid of fish meal protein, corn flour are become to solid fermentation culture medium with alfalfa meal hybrid modulation, and under 10～50 ℃ of conditions, secondary solid state fermentation enzymolysis 6～360h, makes fish meal small peptide feed;
In solid fermentation culture medium, the weight ratio of the little peptide liquid of fish meal protein, corn flour and alfalfa meal is 100:1～50:2～100.
Preferably, in described step liquid fermentation medium 1., the weight ratio of commercially available fish meal inferior, molasses, brown sugar or glucose, water, lactic acid bacteria culturers liquid and saccharomycete strain liquid is 100:5～50:1～10:150～250:3～8:3～8.
Preferably, in described step liquid fermentation medium 1., the addition of microbial protease is 100～500U/g substrate wet basis.
Preferably, described step 1. in the temperature of a liquid fermentation enzymolysis be 35～42 ℃, the time is 48～72h.
Preferably, in described step solid fermentation culture medium 2., the weight ratio of the little peptide liquid of fish meal protein, corn flour and alfalfa meal is 100:5～10:10～20.
Preferably, the described step 2. temperature of middle secondary solid state fermentation enzymolysis is 32～38 ℃, and the time is 72～120h.
The preparation method of fish meal small peptide feed of the present invention, utilize the commercially available fish meal inferior of microbial protease, lactic acid bacteria and saccharomycetes to make fermentation enzymolysis, molasses and brown sugar or glucose, again with corn flour, alfalfa meal mixed culture fermentation enzymolysis after the fish meal small peptide feed that makes not only can eliminate ANFs, pathogenic microbe killing, but also produce abundant little peptide, lactic acid, Yeast protein and probio, cause stink to be eliminated, present the fragrant tart flavour of raw meat, can improve livestock and poultry feed intake, promote piglet growth, improve milking sow lactation amount.
The specific embodiment
Below with reference to embodiment, the present invention is described in detail.
Embodiment 1
By the strong fish meal commercially available inferior of 100kg stink, 10kg molasses, after 5kg brown sugar and microbial protease 0.2kg (300U/g substrate wet basis) mix, add again 180kg clean water, 30kg lactobacillus inoculation liquid and 20kg barms liquid, be modulated into liquid glycolysis culture medium, be warming up to 38 ℃ of-42 ℃ of conditions next time after liquid fermentation enzymolysis 60h, add 60kg corn flour and 250kg alfalfa meal, under 32 ℃ of-38 ℃ of conditions after secondary solid state fermentation enzymolysis 72h, making stink eliminates, present the fish meal small peptide feed (wet basis) of the fragrant tart flavour of raw meat, or oven dry makes fish meal small peptide feed (butt).
In the fish meal small peptide feed (wet basis) of embodiment 1 preparation, nutrition content is as shown in the table:
| Project | Index | Project | Index |
| Color and luster | Yellowish-brown or light yellow | Phosphorus/% | 0.4～1.2 |
| Moisture/% | 36.0～40.0 | Lysine/% | 0.25～0.42 |
| Thick protein/% | 14.0～18.0 | Methionine/% | 0.15～0.30 |
| Crude fat/% | 3.0～6.0 | Little peptide (the heavy albumen of acid)/% | 5.0～10.0 |
| Crude fibre/% | 9.0～12 | Lactic acid/% | 2～4 |
| Coarse ash/% | 0.5～2.5 | Lactic acid bacteria | 50～300×10 8 |
| Calcium/% | 0.5～1.5 | Saccharomycete | 50～300×10 8 |
Embodiment 2
By the strong fish meal commercially available inferior of 100kg stink, 20kg molasses, after 10kg glucose and microbial protease 0.2kg (300U/g substrate wet basis) mix, add again 250kg clean water, 25kg lactobacillus inoculation liquid and 15kg barms liquid, be modulated into liquid glycolysis culture medium, be warming up to 38 ℃ of-42 ℃ of conditions next time after liquid fermentation enzymolysis 72h, add 50kg corn flour and 200kg alfalfa meal, under 32 ℃ of-38 ℃ of conditions after secondary solid state fermentation enzymolysis 96h, making stink eliminates, present the fish meal small peptide feed (wet basis) of the fragrant tart flavour of raw meat, or oven dry makes fish meal small peptide feed (butt).
Embodiment 3
By the strong fish meal commercially available inferior of 500kg stink, 80kg molasses, after 30kg glucose and microbial protease 1.2kg (350U/g substrate wet basis) mix, add again 1500kg clean water, 100kg lactobacillus inoculation liquid and 60kg barms liquid, be modulated into liquid glycolysis culture medium, be warming up to 38 ℃ of-42 ℃ of conditions next time after liquid fermentation enzymolysis 48h, add 300kg corn flour and 800kg alfalfa meal, under 32 ℃ of-38 ℃ of conditions after secondary solid state fermentation enzymolysis 120h, making stink eliminates, present the fish meal small peptide feed (wet basis) of the fragrant tart flavour of raw meat, or oven dry makes fish meal small peptide feed (butt).
Embodiment 4
By the strong fish meal commercially available inferior of 500kg stink, 120kg molasses, after 20kg brown sugar and microbial protease 1.0kg (300U/g substrate wet basis) mix, add again 1800kg clean water, 120kg lactobacillus inoculation liquid and 80kg barms liquid, be modulated into liquid glycolysis culture medium, be warming up to 38 ℃ of-42 ℃ of conditions next time after liquid fermentation enzymolysis 60h, add 200kg corn flour and 1200kg alfalfa meal, under 32 ℃ of-38 ℃ of conditions after secondary solid state fermentation enzymolysis 72h, making stink eliminates, present the fish meal small peptide feed (wet basis) of the fragrant tart flavour of raw meat, or oven dry makes fish meal small peptide feed (butt).
Embodiment 5
By the strong fish meal commercially available inferior of 1000kg stink, 150kg molasses, after 60kg glucose and microbial protease 1.8kg (250U/g substrate wet basis) mix, add again 4000kg clean water, 200kg lactobacillus inoculation liquid and 150kg barms liquid, be modulated into liquid glycolysis culture medium, be warming up to 38 ℃ of-42 ℃ of conditions next time after liquid fermentation enzymolysis 48h, add 500kg corn flour and 2000kg alfalfa meal, under 38 ℃ of-40 ℃ of conditions after secondary solid state fermentation enzymolysis 80h, making stink eliminates, present the fish meal small peptide feed (wet basis) of the fragrant tart flavour of raw meat, or oven dry makes fish meal small peptide feed (butt).
Embodiment 6
By the strong fish meal commercially available inferior of 1000kg stink, 300kg molasses, after 30kg brown sugar and microbial protease 2.4kg (350U/g substrate wet basis) mix, add again 4000kg clean water, 150kg lactobacillus inoculation liquid and 120kg barms liquid, be modulated into liquid glycolysis culture medium, be warming up to 38 ℃ of-42 ℃ of conditions next time after liquid fermentation enzymolysis 36h, add 300kg corn flour and 1500kg alfalfa meal, under 32 ℃ of-38 ℃ of conditions after secondary solid state fermentation enzymolysis 120h, making stink eliminates, present the fish meal small peptide feed (wet basis) of the fragrant tart flavour of raw meat, or oven dry makes fish meal small peptide feed (butt).
Above content is only made for the present invention giving an example and explanation; affiliated those skilled in the art make various modifications to described specific embodiment or supplement or adopt similar mode to substitute; only otherwise depart from the design of invention or surmount this scope as defined in the claims, all should belong to protection scope of the present invention.
Claims (6)
1. a preparation method for fish meal small peptide feed, is characterized in that step is as follows:
1., a liquid fermentation enzymolysis is prepared the little peptide liquid of fish meal protein
Commercially available fish meal inferior, molasses, brown sugar or glucose, water, microbial protease, lactic acid bacteria culturers liquid and saccharomycete strain liquid hybrid modulation are become to liquid fermentation medium, liquid fermentation enzymolysis 2～120h once in 20～70 ℃ of conditions, makes the little peptide liquid of fish meal protein;
In liquid fermentation medium, the weight ratio of commercially available fish meal inferior, molasses, brown sugar or glucose, water, lactic acid bacteria culturers liquid and saccharomycete strain liquid is 100:1～500:0.1～100:100～1000:1～20:1～20, and in liquid fermentation medium, the addition of microbial protease is 0.005～2000 U/g substrate wet basis;
2., the little peptide liquid of fish meal protein secondary solid state fermentation enzymolysis is prepared fish meal small peptide feed
The little peptide liquid of fish meal protein, corn flour are become to solid fermentation culture medium with alfalfa meal hybrid modulation, and under 10～50 ℃ of conditions, secondary solid state fermentation enzymolysis 6～360h, makes fish meal small peptide feed;
In solid fermentation culture medium, the weight ratio of the little peptide liquid of fish meal protein, corn flour and alfalfa meal is 100:1～50:2～100.
2. the preparation method of fish meal small peptide feed according to claim 1, is characterized in that: in described step liquid fermentation medium 1., the weight ratio of commercially available fish meal inferior, molasses, brown sugar or glucose, water, lactic acid bacteria culturers liquid and saccharomycete strain liquid is 100:5～50:1～10:150～250:3～8:3～8.
3. the preparation method of fish meal small peptide feed according to claim 1, is characterized in that: in described step liquid fermentation medium 1., the addition of microbial protease is 100～500 U/g substrate wet basis.
4. the preparation method of fish meal small peptide feed according to claim 1, is characterized in that: described step 1. in the temperature of a liquid fermentation enzymolysis be 35～42 ℃, the time is 48～72h.
5. the preparation method of fish meal small peptide feed according to claim 1, is characterized in that: in described step solid fermentation culture medium 2., the weight ratio of the little peptide liquid of fish meal protein, corn flour and alfalfa meal is 100:5～10:10～20.
6. the preparation method of fish meal small peptide feed according to claim 1, is characterized in that: the described step 2. temperature of middle secondary solid state fermentation enzymolysis is 32～38 ℃, and the time is 72～120h.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104509746A (en) * | 2014-12-23 | 2015-04-15 | 安徽五粮泰生物工程股份有限公司 | Preparation method of lactating sow feed |
| CN106135657A (en) * | 2015-04-20 | 2016-11-23 | 王相生 | Spray-dried plasma protein in feedstuff is replaced based on the protein raw materials of small composite peptide and antibody |
| CN108770993A (en) * | 2018-05-18 | 2018-11-09 | 广西开田三农科技有限责任公司 | A kind of preparation method of polypeptide feed |
| CN110663813A (en) * | 2019-11-07 | 2020-01-10 | 中国海洋大学 | Fish meal microbial fermentation and enzymolysis method |
| CN112167443A (en) * | 2020-09-29 | 2021-01-05 | 秦勇 | Method for preparing pet food for improving immunity of pets and delaying senescence |
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| CN108770993A (en) * | 2018-05-18 | 2018-11-09 | 广西开田三农科技有限责任公司 | A kind of preparation method of polypeptide feed |
| CN110663813A (en) * | 2019-11-07 | 2020-01-10 | 中国海洋大学 | Fish meal microbial fermentation and enzymolysis method |
| CN110663813B (en) * | 2019-11-07 | 2022-04-08 | 中国海洋大学 | Fish meal microbial fermentation and enzymolysis method |
| CN112167443A (en) * | 2020-09-29 | 2021-01-05 | 秦勇 | Method for preparing pet food for improving immunity of pets and delaying senescence |
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