CN105795162A - Fish feed for culturing fish in rice field and preparation method of fish feed - Google Patents
Fish feed for culturing fish in rice field and preparation method of fish feed Download PDFInfo
- Publication number
- CN105795162A CN105795162A CN201610186077.1A CN201610186077A CN105795162A CN 105795162 A CN105795162 A CN 105795162A CN 201610186077 A CN201610186077 A CN 201610186077A CN 105795162 A CN105795162 A CN 105795162A
- Authority
- CN
- China
- Prior art keywords
- parts
- weight
- rice field
- radix glycyrrhizae
- cacumen platycladi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of feeds for aquaculture, in particular to a fish feed for culturing fish in a rice field and a preparation method of the fish feed. According to the fish feed, various feed raw materials are adopted, besides, earthworm dung with high protein quality and cow dung containing massive nutrient elements are used as raw materials, and fermentation treatment is performed so that waste cow dung and the waste earthworm dung are recycled and prepared into a feed; compared with a conventional feed for the fertilizer field, the feed disclosed by the invention has a higher added value. Besides, nutrient components in the earthworm dung and nutrient components in the cow dung are combined, and dicalcium phosphate, microorganism fermentation substances, corn, wheat, traditional Chinese medicine powder, pine needles and the like are added, so that the normal metabolism of gastrointestinal tracts of the fish can be promoted, the nutrient components can be sufficiently absorbed and utilized by organisms, the feed conversion rate is increased, the immunocompetence can also be improved, the occurrence of diseases is reduced, the quality of fish meat is improved, and the growth rate of the fish can be accelerated.
Description
Technical field
The present invention relates to field of feed used for aquiculture, especially a kind of rice field fish culture fish meal and preparation method thereof.
Background technology
Flesh of fish fine and tender taste, nutritious, containing substantial amounts of protein and the trace element of needed by human body, vitamin, lecithin etc., can hypermnesis, thinking and analysis ability, delay mental deterioration, warming middle-JIAO QI invigorating, warming the stomach, profit skin etc., be warming middle-JIAO QI invigorating health-preserving food, is also the topmost protein food source of our people.
In the prior art, in order to make setting up of fish culture pond less costly, expand the profit margin breeded fish, have substantial amounts of researcher to start to be created as the cultivation of fish three-dimensional culture mode, and extensively drawn attention；Also there is the substantial amounts of reported success utilizing rice field fish culture this year, as the water surface just with rice field is breeded fish, namely simply utilize replacement pond, rice field to carry out original putting in a suitable place to breed；But lack scientific and reasonable management and material of throwing something and feeding due to this breeding way so that survival rate and the disease resistance of fish are poor, cause that yield is relatively low, even affect flesh of fish matter；For this, have the pattern of researcher rice field fish culture further to carry out further optimization process, such as " Hehua fish sulturing method in rice field " that number of patent application is 00116054.0.
However, prior art is concentrated mainly in the pattern of rice field fish culture for the research of rice field fish culture, and in the process of rice field fish culture, how by nutrition supply and disease control and aquaculture model to combination, reach to improve the purpose of rice field fish culture quality；Also this it is based on, this researcher is on the basis of existing rice field fish culture pattern, by to the adjustment needing the formula of feedstuff and the preparation technology thrown in rice field fish culture process, making to obtain a kind of rice field fish culture fish meal, this feedstuff can be effectively combined rice field fish culture pattern of the prior art, reaches fish carries out nutrition supply and meat improvement, reduce disease rate, improve survival rate, increase profit margin, promote the fast development of aquaculture industry.
Summary of the invention
In order to solve the above-mentioned technical problem existed in prior art, the present invention provides a kind of rice field fish culture fish meal and preparation method thereof.
It is achieved particular by techniques below scheme:
A kind of rice field fish culture fish meal, its material composition with parts by weight be wormcast 20-30 part, cattle manure 100-200 part, Chinese medicine powder 17-27 part, cobalt blue pigment 23-35 part, microbial fermenters 3-9 part, Semen Maydis 5-11 part, Semen Tritici aestivi 2-7 part, calcium hydrogen phosphate 3-5 part, Folium Pini 1-7 part.
Described material composition with parts by weight be wormcast 25 parts, cattle manure 150 parts, Chinese medicine powder 22 parts, cobalt blue pigment 27 parts, microbial fermenters 6 parts, Semen Maydis 9 parts, Semen Tritici aestivi 5 parts, calcium hydrogen phosphate 4 parts, 5 parts of Folium Pini.
Described Chinese medicine powder, its material composition with parts by weight be Radix Notoginseng 2-7 part, Sargassum 11-15 part, Radix Glycyrrhizae 3-5 part, Herba Houttuyniae 1-5 part, Cacumen Platycladi 1-3 part.
Described Chinese medicine powder, its material composition with parts by weight be Radix Notoginseng 5 parts, Sargassum 13 parts, 4 parts of Radix Glycyrrhizae, Herba Houttuyniae 3 parts, Cacumen Platycladi 2 parts.
Described Chinese medicine powder, its preparation method comprises the steps:
(1) by Radix Notoginseng under the dry environment of 35-50 DEG C, process and turn yellow to blade face, become flavescence Radix Notoginseng；Being put into by flavescence Radix Notoginseng in 2-3 times of weight, the warm water of 40-50 DEG C again, constant temperature soaks 10-15h, filters, and collects and soaks Radix Notoginseng；Immersion Radix Notoginseng is dried at 45-60 DEG C water content less than 8% again, is ground into 100-150 order fine powder, obtains Radix Notoginseng powder；
(2) after Sargassum and Herba Houttuyniae being mixed after steaming stir-fry, sterilization, it is ground into the fine powder that particle diameter is 2.5-4.5nm, obtains just mixed powder；
(3) Radix Glycyrrhizae is cleaned, put in 2-3 times of weight, 45-60 DEG C of water immersion 8-15h, be then processed into the Radix Glycyrrhizae slurry of more than 80 orders together, then Radix Glycyrrhizae slurry is boiled boil 20-30min, filter, collect Radix Glycyrrhizae filtrate；After Radix Glycyrrhizae filtrate is down to room temperature, adds the ethyl acetate of equal volume, stir process 25-40min, then layering, filtration, collect licorice water extract；The ethyl acetate accounting for licorice water extract 1/2 volume being joined processes once again in licorice water extract, filter, and collects Radix Glycyrrhizae secondary Aqueous extracts；By spray-dried for Radix Glycyrrhizae secondary Aqueous extracts, obtain Radix Glycyrrhizae powder；
(4) Cacumen Platycladi cleaned, be filtered dry, join in clear water equiponderant with it, boil extraction 1.0-2.5h, filter, collect Cacumen Platycladi Aqueous extracts；In Cacumen Platycladi slag, add the low polar organic solvent accounting for its original weight amount 1.5-3.0 times again, boil extraction 1.5-3.0h, filter, collect the low polar organic solvent extracting solution of Cacumen Platycladi；Low for the Cacumen Platycladi of Cacumen Platycladi Aqueous extracts and 1/2 volume polar organic solvent extracting solution is mixed, quiet 10-15h of putting at 0-10 DEG C, filter, collect clear liquor；Again low with the Cacumen Platycladi of remaining 1/2 volume for clear liquor polar organic solvent extracting solution is mixed, at 50-75 DEG C, be concentrated into the concentrated solution that solid content is 1.25-1.60mg/10ml；Then adding the kieselguhr accounting for solid content weight 20-35% in concentrated solution, stir 10-18h, filter at 45-60 DEG C, collection processes concentrated solution；Process concentrated solution is condensed into fluid extract at 50-75 DEG C, obtains Cacumen Platycladi extractum；
(5) by after the Radix Notoginseng powder of above-mentioned preparation, just mixed powder, Radix Glycyrrhizae powder, Cacumen Platycladi extractum mix homogeneously, at 45-60 DEG C, it is dried to water content less than 2%, pulverizes, obtain Chinese medicine powder.
Described microbial fermenters be one or more in acetobacter, yeast, lactobacillus after continuously activation, total living bacteria count is 8.1 × 109cfu/g-9×1012The fermented product of cfu/g.
Described microbial fermenters is by acetobacter, yeast, the lactobacillus continuous fermented product activated of warp respectively according to (0.1-0.15): (2-2.5): the part by weight of (0.2-0.3) mixes, its total living bacteria count is 8.1 × 109cfu/g-9×1012cfu/g。
Described cobalt blue pigment is one or more in rye grass, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Medicaginis leaf, maize seedling, weeds, when for, time two or more, the ratio of each component is equal portions or other ratio.
Described cobalt blue pigment is to process after 1-3h through 70-90 DEG C, room temperature dry in the shade to moisture be the grass of 1-6%.
The preparation method specifically also providing for rice field fish culture fish meal, comprises the following steps:
(1) by after wormcast, cattle manure mix homogeneously, add calcium hydrogen phosphate and microbial fermenters, and to adopt mixing speed be that 100-300r/min stirs 20-30min, then it be sent in fermentation vat, adjust temperature and be 60-80 DEG C, pH value is 3.5-7, humidity is 55-80% fermentation process 20-30 days, and every 3 days turning and throwing once, after fermentation completes, it is added thereto to Folium Pini and Chinese medicine powder mix homogeneously, obtains just mixed thing；
(2) by Semen Maydis, Semen Tritici aestivi mixed grinding the sieve crossing 150-300 order, take undersize, by undersize and the clear water accounting for its weight 10-28%, stir, then through expanding treatment, obtain puffing material；Then in puffing material, add the water accounting for its weight 30-40% again, stir evenly form slurry；
(3) half of the slurry that step (2) prepares is taken; its first mixed thing obtained with step (1) is mixed; and it is stirred for mix homogeneously; it is sent to pelletize in comminutor again; and after granule is placed 1-3 days in the environment that temperature is 25-33 DEG C; it is placed in the environment that humidity is 80-90% again; controlling temperature is 1-5 DEG C of placement 1-3h; again by remaining half slurry even application at particle surface; adopting temperature to be 55-60 DEG C again, to dry be 5% to moisture, obtains rice field fish culture fish meal.
Compared with prior art, the technique effect of the present invention is embodied in:
The fish meal of the present invention have employed multiple feedstuff, and using the wormcast with high protein quality and the cattle manure containing macronutrient as raw material, after being carried out fermentation process, make that discarded cattle manure is carried out recycling with wormcast and prepare into feedstuff, comparing and be conventionally used to fertilizer field, it has higher added value；And in conjunction with the nutritional labeling in wormcast and cattle manure, add calcium hydrogen phosphate, microbial fermenters, Semen Maydis, Semen Tritici aestivi, Chinese medicine powder, Folium Pini etc., the gastrointestinal tract homergy of fish can be promoted, its nutrition is enable fully to be absorbed by organisms utilization, improve food conversion ratio, moreover it is possible to strengthen immunocompetence, reduce disease and occur, improve the quality of the flesh of fish, and the speed of growth of Chinese herring can be added.Especially the Folium Pini being directly added into can provide fiber, it is possible to effectively improves the digestive system of fish, improves the fry absorption to nutritional labeling, and in conjunction with the addition of Chinese medicine powder Yu Folium Pini so that the disease-resistant performance of fish is improved, and reduces sickness rate, improve survival rate, reduce cost.
The present invention specifically carries out rice field fish culture also by the above-mentioned feedstuff of employing and traditional feedstuff is breeded fish and carried out contrast experiment, its result is: adopt the feedstuff of the present invention to carry out rice field fish culture, can effectively improve the yield of fish, the weight average of 1 year wall scroll fish of cultivation, at 3-3.5kg, exceeds 5-7 percentage point than traditional；And the mortality rate in breeding process is more significant lower than traditional, about low 10-20 percentage point so that cultivation profit improves more than 50%.
The present invention is importantly by preparation process, control in conjunction with the nutritive value in material composition, the addition step of each raw material is defined, and in conjunction with the restriction of the control of temperature and other technological parameters, make each nutritional labeling in raw material in carrying out the process mixed, occur accurately, directed biochemistry, physical blending effect, nutritive value in fish meal is improved, and can effectively improve the gastrointestinal system of fish meal, promote fry food feedstuff, reduce the loss of material of throwing something and feeding, and under the Folium Pini synergism with Chinese medicine powder, reach the epidemic prevention to fry, reduce sickness rate and avoid independent medication epidemic prevention, reduce rice field fish culture cost, improve profit.
Detailed description of the invention
Below in conjunction with specific embodiment technical scheme done further restriction, but the scope claimed is not only limited to the description made.
The concrete preparation method of the various microbial fermenters in the microbial fermenters of the present invention is:
Prepared by acetobacter fermented product: after Testa oryzae, peptone, oligosaccharide, Semen Maydis powder being mixed with the part by weight of 4: 1.5: 0.35: 2, acetobacter solid-state fermentation culture medium is made in the steaming and decocting that adds water, then acetobacter solid-state fermentation culture medium is loaded and test tube accesses acetobacter, inoculum concentration is the 1.5% of acetobacter solid-state fermentation culture medium weight, at 35 DEG C, cultivate 40h, continuously after activation, obtain acetobacter fermented product (that is: acetobacter fermented bacterium).
After Testa oryzae, peptone, oligosaccharide, edible fungi heel are mixed by the preparation of yeast fermentation thing with the part by weight of 4: 1.5: 0.3: 1, yeast solid-state fermentation culture medium is made in the steaming and decocting that adds water, then microzyme culture medium is loaded access yeast in test tube, inoculum concentration is the 1.25% of yeast solid-state fermentation culture medium weight, at 40 DEG C, cultivate 40h, continuously after activation, obtain yeast fermentation thing (that is: yeast fermentation strain).
Prepared by lactobacillus fermented product: after Testa oryzae, peptone, oligosaccharide, wheat bran being mixed with the part by weight of 4: 1.5: 0.3: 2, lactobacillus solid-state fermentation culture medium is made in the steaming and decocting that adds water, then lactobacillus solid-state fermentation culture medium is loaded and test tube accesses lactobacillus, inoculum concentration is the 2% of lactobacillus solid-state fermentation culture medium weight, at 40 DEG C, cultivate 45h, continuously after activation, obtain lactobacillus fermented product (that is: lactobacillus fermentation strain).
Except above-mentioned processing method, it is it may also is that by Testa oryzae, wheat bran, oligosaccharide, inorganic salt, after edible fungi heel mixes with the part by weight of 5: 3: 0.3: 0.15: 1, fermentable material is made in the steaming and decocting that adds water, again fermentable material is put in fermentation vat, then the above-mentioned acetobacter fermented bacterium mixed according to the part by weight of 0.2: 0.8: 0.3 is accessed, yeast fermentation strain and lactobacillus fermentation strain, at 35 DEG C, moisturizing is fermented 8 days, after fermenting, dry 6h at 65 DEG C, obtain microbial fermenters, its total living bacteria count is 6 × 109cfu/g-8×1012cfu/g。
Embodiment 1
A kind of rice field fish culture fish meal, its material composition is wormcast 20kg, cattle manure 100kg, Chinese medicine powder 17kg, cobalt blue pigment 23kg, microbial fermenters 3kg, Semen Maydis 5kg, Semen Tritici aestivi 2kg, calcium hydrogen phosphate 3kg, Folium Pini 1kg by weight.
Described Chinese medicine powder, its material composition is Radix Notoginseng 2kg, Sargassum 11kg, Radix Glycyrrhizae 3kg, Herba Houttuyniae 1kg, Cacumen Platycladi 1kg by weight.
Described Chinese medicine powder, its preparation method comprises the steps:
(1) by Radix Notoginseng under the dry environment of 35 DEG C, process and turn yellow to blade face, become flavescence Radix Notoginseng；Being put into by flavescence Radix Notoginseng in 2 times of weight, the warm water of 40 DEG C again, constant temperature soaks 10h, filters, and collects and soaks Radix Notoginseng；Immersion Radix Notoginseng is dried at 45 DEG C water content less than 8% again, is ground into 100 order fine powders, obtains Radix Notoginseng powder；
(2) after Sargassum and Herba Houttuyniae being mixed after steaming stir-fry, sterilization, it is ground into the fine powder that particle diameter is 2.5nm, obtains just mixed powder；
(3) Radix Glycyrrhizae is cleaned, put in 2 times of weight, 45 DEG C of water immersion 8h, be then processed into the Radix Glycyrrhizae slurry of more than 80 orders together, then Radix Glycyrrhizae slurry is boiled boil 20min, filter, collect Radix Glycyrrhizae filtrate；After Radix Glycyrrhizae filtrate is down to room temperature, adds the ethyl acetate of equal volume, stir process 25min, then layering, filtration, collect licorice water extract；The ethyl acetate accounting for licorice water extract 1/2 volume being joined processes once again in licorice water extract, filter, and collects Radix Glycyrrhizae secondary Aqueous extracts；By spray-dried for Radix Glycyrrhizae secondary Aqueous extracts, obtain Radix Glycyrrhizae powder；
(4) Cacumen Platycladi cleaned, be filtered dry, join in clear water equiponderant with it, boil extraction 1.0h, filter, collect Cacumen Platycladi Aqueous extracts；In Cacumen Platycladi slag, add the low polar organic solvent (petroleum ether) accounting for its original weight amount 1.5 times again, boil extraction 1.5h, filter, collect the low polar organic solvent extracting solution of Cacumen Platycladi；Low for the Cacumen Platycladi of Cacumen Platycladi Aqueous extracts and 1/2 volume polar organic solvent extracting solution is mixed, quiet 10h of putting at 0-10 DEG C, filter, collect clear liquor；Again low with the Cacumen Platycladi of remaining 1/2 volume for clear liquor polar organic solvent extracting solution is mixed, at 50 DEG C, be concentrated into the concentrated solution that solid content is 1.25mg/10ml；Then adding the kieselguhr accounting for solid content weight 20% in concentrated solution, stir 10h, filter at 45 DEG C, collection processes concentrated solution；Process concentrated solution is condensed into fluid extract at 50 DEG C, obtains Cacumen Platycladi extractum；
(5) by after the Radix Notoginseng powder of above-mentioned preparation, just mixed powder, Radix Glycyrrhizae powder, Cacumen Platycladi extractum mix homogeneously, at 45 DEG C, it is dried to water content less than 2%, pulverizes, obtain Chinese medicine powder.
Described microbial fermenters is acetobacter.
Described cobalt blue pigment is rye grass.
Embodiment 2
A kind of rice field fish culture fish meal, its material composition is wormcast 30kg, cattle manure 200kg, Chinese medicine powder 27kg, cobalt blue pigment 35kg, microbial fermenters 9kg, Semen Maydis 11kg, Semen Tritici aestivi 7kg, calcium hydrogen phosphate 5kg, Folium Pini 7kg by weight.
Described Chinese medicine powder, its material composition is Radix Notoginseng 7kg, Sargassum 15kg, Radix Glycyrrhizae 5kg, Herba Houttuyniae 5kg, Cacumen Platycladi 3kg by weight.
Described Chinese medicine powder, its preparation method comprises the steps:
(1) by Radix Notoginseng under the dry environment of 50 DEG C, process and turn yellow to blade face, become flavescence Radix Notoginseng；Being put into by flavescence Radix Notoginseng in 3 times of weight, the warm water of 50 DEG C again, constant temperature soaks 15h, filters, and collects and soaks Radix Notoginseng；Immersion Radix Notoginseng is dried at 60 DEG C water content less than 8% again, is ground into 150 order fine powders, obtains Radix Notoginseng powder；
(2) after Sargassum and Herba Houttuyniae being mixed after steaming stir-fry, sterilization, it is ground into the fine powder that particle diameter is 4.5nm, obtains just mixed powder；
(3) Radix Glycyrrhizae is cleaned, put in 3 times of weight, 60 DEG C of water immersion 15h, be then processed into the Radix Glycyrrhizae slurry of more than 80 orders together, then Radix Glycyrrhizae slurry is boiled boil 30min, filter, collect Radix Glycyrrhizae filtrate；After Radix Glycyrrhizae filtrate is down to room temperature, adds the ethyl acetate of equal volume, stir process 40min, then layering, filtration, collect licorice water extract；The ethyl acetate accounting for licorice water extract 1/2 volume being joined processes once again in licorice water extract, filter, and collects Radix Glycyrrhizae secondary Aqueous extracts；By spray-dried for Radix Glycyrrhizae secondary Aqueous extracts, obtain Radix Glycyrrhizae powder；
(4) Cacumen Platycladi cleaned, be filtered dry, join in clear water equiponderant with it, boil extraction 2.5h, filter, collect Cacumen Platycladi Aqueous extracts；In Cacumen Platycladi slag, add the low polar organic solvent (No. six solvent naphthas) accounting for its original weight amount 3.0 times again, boil extraction 3.0h, filter, collect the low polar organic solvent extracting solution of Cacumen Platycladi；Low for the Cacumen Platycladi of Cacumen Platycladi Aqueous extracts and 1/2 volume polar organic solvent extracting solution is mixed, quiet 15h of putting at 0-10 DEG C, filter, collect clear liquor；Again low with the Cacumen Platycladi of remaining 1/2 volume for clear liquor polar organic solvent extracting solution is mixed, at 75 DEG C, be concentrated into the concentrated solution that solid content is 1.60mg/10ml；Then adding the kieselguhr accounting for solid content weight 35% in concentrated solution, stir 18h, filter at 60 DEG C, collection processes concentrated solution；Process concentrated solution is condensed into fluid extract at 75 DEG C, obtains Cacumen Platycladi extractum；
(5) by after the Radix Notoginseng powder of above-mentioned preparation, just mixed powder, Radix Glycyrrhizae powder, Cacumen Platycladi extractum mix homogeneously, at 60 DEG C, it is dried to water content less than 2%, pulverizes, obtain Chinese medicine powder.
Described microbial fermenters is yeast after activation continuously, total living bacteria count is 8.1 × 109cfu/g-9×1012The fermented product of cfu/g.
Described cobalt blue pigment is Herba Astragali Melilotoidis (Herba Astragali Sinici).
Described cobalt blue pigment is to process after 1h through 70 DEG C, room temperature dry in the shade to moisture be the grass of 1%.
Embodiment 3
A kind of rice field fish culture fish meal, its material composition is wormcast 25kg, cattle manure 150kg, Chinese medicine powder 22kg, cobalt blue pigment 27kg, microbial fermenters 6kg, Semen Maydis 9kg, Semen Tritici aestivi 5kg, calcium hydrogen phosphate 4kg, Folium Pini 5kg by weight.
Described Chinese medicine powder, its material composition is Radix Notoginseng 5kg, Sargassum 13kg, Radix Glycyrrhizae 4kg, Herba Houttuyniae 3kg, Cacumen Platycladi 2kg by weight.
Described Chinese medicine powder, its preparation method comprises the steps:
(1) by Radix Notoginseng under the dry environment of 40 DEG C, process and turn yellow to blade face, become flavescence Radix Notoginseng；Being put into by flavescence Radix Notoginseng in 3 times of weight, the warm water of 45 DEG C again, constant temperature soaks 13h, filters, and collects and soaks Radix Notoginseng；Immersion Radix Notoginseng is dried at 55 DEG C water content less than 8% again, is ground into 130 order fine powders, obtains Radix Notoginseng powder；
(2) after Sargassum and Herba Houttuyniae being mixed after steaming stir-fry, sterilization, it is ground into the fine powder that particle diameter is 3.5nm, obtains just mixed powder；
(3) Radix Glycyrrhizae is cleaned, put in 2.5 times of weight, 50 DEG C of water immersion 11h, be then processed into the Radix Glycyrrhizae slurry of more than 80 orders together, then Radix Glycyrrhizae slurry is boiled boil 25min, filter, collect Radix Glycyrrhizae filtrate；After Radix Glycyrrhizae filtrate is down to room temperature, adds the ethyl acetate of equal volume, stir process 30min, then layering, filtration, collect licorice water extract；The ethyl acetate accounting for licorice water extract 1/2 volume being joined processes once again in licorice water extract, filter, and collects Radix Glycyrrhizae secondary Aqueous extracts；By spray-dried for Radix Glycyrrhizae secondary Aqueous extracts, obtain Radix Glycyrrhizae powder；
(4) Cacumen Platycladi cleaned, be filtered dry, join in clear water equiponderant with it, boil extraction 1.5h, filter, collect Cacumen Platycladi Aqueous extracts；In Cacumen Platycladi slag, add the low polar organic solvent accounting for its original weight amount 2.5 times again, boil extraction 2.0h, filter, collect the low polar organic solvent extracting solution of Cacumen Platycladi；Low for the Cacumen Platycladi of Cacumen Platycladi Aqueous extracts and 1/2 volume polar organic solvent extracting solution is mixed, quiet 13h of putting at 0-10 DEG C, filter, collect clear liquor；Again low with the Cacumen Platycladi of remaining 1/2 volume for clear liquor polar organic solvent extracting solution is mixed, at 65 DEG C, be concentrated into the concentrated solution that solid content is 1.550mg/10ml；Then adding the kieselguhr accounting for solid content weight 25% in concentrated solution, stir 17h, filter at 55 DEG C, collection processes concentrated solution；Process concentrated solution is condensed into fluid extract at 65 DEG C, obtains Cacumen Platycladi extractum；
(5) by after the Radix Notoginseng powder of above-mentioned preparation, just mixed powder, Radix Glycyrrhizae powder, Cacumen Platycladi extractum mix homogeneously, at 55 DEG C, it is dried to water content less than 2%, pulverizes, obtain Chinese medicine powder.
Described microbial fermenters is to be mixed according to the part by weight of 0.1: 2.5: 0.3 through the fermented product of activation continuously respectively by acetobacter, yeast, lactobacillus, and its total living bacteria count is 8.1 × 109cfu/g-9×1012cfu/g。
Described cobalt blue pigment is rye grass, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Medicaginis leaf, maize seedling, weeds, the ratio of each component is equal portions or other ratio.
Described cobalt blue pigment is to process after 2h through 80 DEG C, room temperature dry in the shade to moisture be the grass of 5%.
Embodiment 4
A kind of rice field fish culture fish meal, its material composition is wormcast 20kg, cattle manure 160kg, Chinese medicine powder 19kg, cobalt blue pigment 27kg, microbial fermenters 6kg, Semen Maydis 5kg, Semen Tritici aestivi 7kg, calcium hydrogen phosphate 4kg, Folium Pini 6kg by weight.
Described Chinese medicine powder, its material composition is Radix Notoginseng 2kg, Sargassum 13kg, Radix Glycyrrhizae 4kg, Herba Houttuyniae 3kg, Cacumen Platycladi 3kg by weight.
Described Chinese medicine powder, its preparation method comprises the steps:
(1) by Radix Notoginseng under the dry environment of 45 DEG C, process and turn yellow to blade face, become flavescence Radix Notoginseng；Being put into by flavescence Radix Notoginseng in 2 times of weight, the warm water of 50 DEG C again, constant temperature soaks 13h, filters, and collects and soaks Radix Notoginseng；Immersion Radix Notoginseng is dried at 60 DEG C water content less than 8% again, is ground into 130 order fine powders, obtains Radix Notoginseng powder；
(2) after Sargassum and Herba Houttuyniae being mixed after steaming stir-fry, sterilization, it is ground into the fine powder that particle diameter is 3.5nm, obtains just mixed powder；
(3) Radix Glycyrrhizae is cleaned, put in 3 times of weight, 45 DEG C of water immersion 8h, be then processed into the Radix Glycyrrhizae slurry of more than 80 orders together, then Radix Glycyrrhizae slurry is boiled boil 30min, filter, collect Radix Glycyrrhizae filtrate；After Radix Glycyrrhizae filtrate is down to room temperature, adds the ethyl acetate of equal volume, stir process 40min, then layering, filtration, collect licorice water extract；The ethyl acetate accounting for licorice water extract 1/2 volume being joined processes once again in licorice water extract, filter, and collects Radix Glycyrrhizae secondary Aqueous extracts；By spray-dried for Radix Glycyrrhizae secondary Aqueous extracts, obtain Radix Glycyrrhizae powder；
(4) Cacumen Platycladi cleaned, be filtered dry, join in clear water equiponderant with it, boil extraction 1.0h, filter, collect Cacumen Platycladi Aqueous extracts；In Cacumen Platycladi slag, add the low polar organic solvent accounting for its original weight amount 3.0 times again, boil extraction 1.5h, filter, collect the low polar organic solvent extracting solution of Cacumen Platycladi；Low for the Cacumen Platycladi of Cacumen Platycladi Aqueous extracts and 1/2 volume polar organic solvent extracting solution is mixed, quiet 11h of putting at 0-10 DEG C, filter, collect clear liquor；Again low with the Cacumen Platycladi of remaining 1/2 volume for clear liquor polar organic solvent extracting solution is mixed, at 75 DEG C, be concentrated into the concentrated solution that solid content is 1.25mg/10ml；Then adding the kieselguhr accounting for solid content weight 29% in concentrated solution, stir 17h, filter at 55 DEG C, collection processes concentrated solution；Process concentrated solution is condensed into fluid extract at 59 DEG C, obtains Cacumen Platycladi extractum；
(5) by after the Radix Notoginseng powder of above-mentioned preparation, just mixed powder, Radix Glycyrrhizae powder, Cacumen Platycladi extractum mix homogeneously, at 49 DEG C, it is dried to water content less than 2%, pulverizes, obtain Chinese medicine powder.
Described microbial fermenters is to be mixed according to the part by weight of 0.15: 2: 0.2 through the fermented product of activation continuously respectively by acetobacter, yeast, lactobacillus, and its total living bacteria count is 8.1 × 109cfu/g-9×1012cfu/g。
Described cobalt blue pigment is rye grass, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Medicaginis leaf, the ratio of each component is equal portions or other ratio.
Described cobalt blue pigment is to process after 2h through 90 DEG C, room temperature dry in the shade to moisture be the grass of 3%.
Embodiment 5
On the basis of embodiment 1, other are all with embodiment 1, described microbial fermenters is to be mixed according to the part by weight of 0.13: 2.3: 0.25 through the fermented product of activation continuously respectively by acetobacter, yeast, lactobacillus, and its total living bacteria count is 8.1 × 109cfu/g-9×1012cfu/g。
Embodiment 6
On the basis of embodiment 2, other all with embodiment 2, the preparation method specifically also providing for rice field fish culture fish meal, comprise the following steps:
(1) by after wormcast, cattle manure mix homogeneously, add calcium hydrogen phosphate and microbial fermenters, and to adopt mixing speed be 100, r/min stirring 20min, it is sent to again in fermentation vat, adjusting temperature is 60 DEG C, and pH value is 3.5, and humidity is 55% fermentation process 20 days, and every 3 days turning and throwing once, after fermentation completes, it is added thereto to Folium Pini and Chinese medicine powder mix homogeneously, obtains just mixed thing；
(2) by Semen Maydis, Semen Tritici aestivi mixed grinding the sieve crossing 150 orders, take undersize, by undersize and the clear water accounting for its weight 10%, stir, then through expanding treatment, obtain puffing material；Then in puffing material, add the water accounting for its weight 30% again, stir evenly form slurry；
(3) half of the slurry that step (2) prepares is taken; its first mixed thing obtained with step (1) is mixed; and it is stirred for mix homogeneously; it is sent to pelletize in comminutor again; and after granule is placed 1 day in the environment that temperature is 25 DEG C; it is placed in the environment that humidity is 80% again; controlling temperature is 1 DEG C of placement 1h; again by remaining half slurry even application at particle surface; adopting temperature to be 55 DEG C again, to dry be 5% to moisture, obtains rice field fish culture fish meal.
Embodiment 7
On the basis of embodiment 3, other all with embodiment 3, the preparation method specifically also providing for rice field fish culture fish meal, comprise the following steps:
(1) by after wormcast, cattle manure mix homogeneously, add calcium hydrogen phosphate and microbial fermenters, and to adopt mixing speed be that 300r/min stirs 30min, then being sent in fermentation vat, adjusting temperature is 80 DEG C, pH value is 7, humidity is 80% fermentation process 30 days, and every 3 days turning and throwing once, after fermentation completes, it is added thereto to Folium Pini and Chinese medicine powder mix homogeneously, obtains just mixed thing；
(2) by Semen Maydis, Semen Tritici aestivi mixed grinding the sieve crossing 300 orders, take undersize, by undersize and the clear water accounting for its weight 28%, stir, then through expanding treatment, obtain puffing material；Then in puffing material, add the water accounting for its weight 40% again, stir evenly form slurry；
(3) half of the slurry that step (2) prepares is taken; its first mixed thing obtained with step (1) is mixed; and it is stirred for mix homogeneously; it is sent to pelletize in comminutor again; and after granule is placed 3 days in the environment that temperature is 33 DEG C; it is placed in the environment that humidity is 90% again; controlling temperature is 5 DEG C of placement 3h; again by remaining half slurry even application at particle surface; adopting temperature to be 60 DEG C again, to dry be 5% to moisture, obtains rice field fish culture fish meal.
Embodiment 8
On the basis of embodiment 4, other all with embodiment 4, the preparation method specifically also providing for rice field fish culture fish meal, comprise the following steps:
(1) by after wormcast, cattle manure mix homogeneously, add calcium hydrogen phosphate and microbial fermenters, and to adopt mixing speed be that 200r/min stirs 25min, then being sent in fermentation vat, adjusting temperature is 70 DEG C, pH value is 5, humidity is 70% fermentation process 25 days, and every 3 days turning and throwing once, after fermentation completes, it is added thereto to Folium Pini and Chinese medicine powder mix homogeneously, obtains just mixed thing；
(2) by Semen Maydis, Semen Tritici aestivi mixed grinding the sieve crossing 200 orders, take undersize, by undersize and the clear water accounting for its weight 19%, stir, then through expanding treatment, obtain puffing material；Then in puffing material, add the water accounting for its weight 35% again, stir evenly form slurry；
(3) half of the slurry that step (2) prepares is taken; its first mixed thing obtained with step (1) is mixed; and it is stirred for mix homogeneously; it is sent to pelletize in comminutor again; and after granule is placed 2 days in the environment that temperature is 29 DEG C; it is placed in the environment that humidity is 85% again; controlling temperature is 3 DEG C of placement 2h; again by remaining half slurry even application at particle surface; adopting temperature to be 57 DEG C again, to dry be 5% to moisture, obtains rice field fish culture fish meal.
Claims (10)
1. a rice field fish culture fish meal, it is characterized in that, its material composition with parts by weight be wormcast 20-30 part, cattle manure 100-200 part, Chinese medicine powder 17-27 part, cobalt blue pigment 23-35 part, microbial fermenters 3-9 part, Semen Maydis 5-11 part, Semen Tritici aestivi 2-7 part, calcium hydrogen phosphate 3-5 part, Folium Pini 1-7 part.
2. rice field fish culture fish meal as claimed in claim 1, it is characterized in that, described material composition with parts by weight be wormcast 25 parts, cattle manure 150 parts, Chinese medicine powder 22 parts, cobalt blue pigment 27 parts, microbial fermenters 6 parts, Semen Maydis 9 parts, Semen Tritici aestivi 5 parts, calcium hydrogen phosphate 4 parts, 5 parts of Folium Pini.
3. rice field fish culture fish meal as claimed in claim 1 or 2, it is characterised in that described Chinese medicine powder, its material composition with parts by weight be Radix Notoginseng 2-7 part, Sargassum 11-15 part, Radix Glycyrrhizae 3-5 part, Herba Houttuyniae 1-5 part, Cacumen Platycladi 1-3 part.
4. rice field fish culture fish meal as claimed in claim 3, it is characterised in that described Chinese medicine powder, its material composition with parts by weight be Radix Notoginseng 5 parts, Sargassum 13 parts, 4 parts of Radix Glycyrrhizae, Herba Houttuyniae 3 parts, Cacumen Platycladi 2 parts.
5. rice field fish culture fish meal as claimed in claim 3, it is characterised in that described Chinese medicine powder, its preparation method comprises the steps:
(1) by Radix Notoginseng under the dry environment of 35-50 DEG C, process and turn yellow to blade face, become flavescence Radix Notoginseng；Being put into by flavescence Radix Notoginseng in 2-3 times of weight, the warm water of 40-50 DEG C again, constant temperature soaks 10-15h, filters, and collects and soaks Radix Notoginseng；Immersion Radix Notoginseng is dried at 45-60 DEG C water content less than 8% again, is ground into 100-150 order fine powder, obtains Radix Notoginseng powder；
(2) after Sargassum and Herba Houttuyniae being mixed after steaming stir-fry, sterilization, it is ground into the fine powder that particle diameter is 2.5-4.5nm, obtains just mixed powder；
(3) Radix Glycyrrhizae is cleaned, put in 2-3 times of weight, 45-60 DEG C of water immersion 8-15h, be then processed into the Radix Glycyrrhizae slurry of more than 80 orders together, then Radix Glycyrrhizae slurry is boiled boil 20-30min, filter, collect Radix Glycyrrhizae filtrate；After Radix Glycyrrhizae filtrate is down to room temperature, adds the ethyl acetate of equal volume, stir process 25-40min, then layering, filtration, collect licorice water extract；The ethyl acetate accounting for licorice water extract 1/2 volume being joined processes once again in licorice water extract, filter, and collects Radix Glycyrrhizae secondary Aqueous extracts；By spray-dried for Radix Glycyrrhizae secondary Aqueous extracts, obtain Radix Glycyrrhizae powder；
(4) Cacumen Platycladi cleaned, be filtered dry, join in clear water equiponderant with it, boil extraction 1.0-2.5h, filter, collect Cacumen Platycladi Aqueous extracts；In Cacumen Platycladi slag, add the low polar organic solvent accounting for its original weight amount 1.5-3.0 times again, boil extraction 1.5-3.0h, filter, collect the low polar organic solvent extracting solution of Cacumen Platycladi；Low for the Cacumen Platycladi of Cacumen Platycladi Aqueous extracts and 1/2 volume polar organic solvent extracting solution is mixed, quiet 10-15h of putting at 0-10 DEG C, filter, collect clear liquor；Again low with the Cacumen Platycladi of remaining 1/2 volume for clear liquor polar organic solvent extracting solution is mixed, at 50-75 DEG C, be concentrated into the concentrated solution that solid content is 1.25-1.60mg/10ml；Then adding the kieselguhr accounting for solid content weight 20-35% in concentrated solution, stir 10-18h, filter at 45-60 DEG C, collection processes concentrated solution；Process concentrated solution is condensed into fluid extract at 50-75 DEG C, obtains Cacumen Platycladi extractum；
(5) by after the Radix Notoginseng powder of above-mentioned preparation, just mixed powder, Radix Glycyrrhizae powder, Cacumen Platycladi extractum mix homogeneously, at 45-60 DEG C, it is dried to water content less than 2%, pulverizes, obtain Chinese medicine powder.
6. rice field fish culture fish meal as claimed in claim 1 or 2, it is characterised in that described microbial fermenters be one or more in acetobacter, yeast, lactobacillus after activating continuously, total living bacteria count is 8.1 × 109cfu/g-9×1012The fermented product of cfu/g.
7. rice field fish culture fish meal as claimed in claim 1 or 2, it is characterized in that, described microbial fermenters is by acetobacter, yeast, the lactobacillus continuous fermented product activated of warp respectively according to (0.1-0.15): (2-2.5): the part by weight of (0.2-0.3) mixes, its total living bacteria count is 8.1 × 109cfu/g-9×1012cfu/g。
8. rice field fish culture fish meal as claimed in claim 1 or 2, it is characterised in that described cobalt blue pigment is one or more in rye grass, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Medicaginis leaf, maize seedling, weeds, when for, time two or more, the ratio of each component is equal portions or other ratio.
9. rice field fish culture fish meal as claimed in claim 1 or 2, it is characterised in that described cobalt blue pigment is after 70-90 DEG C processes 1-3h, room temperature dry in the shade to moisture be the grass of 1-6%.
10. the preparation method of the rice field fish culture fish meal as described in any one of claim 1-9, it is characterised in that comprise the following steps:
(1) by after wormcast, cattle manure mix homogeneously, add calcium hydrogen phosphate and microbial fermenters, and to adopt mixing speed be that 100-300r/min stirs 20-30min, then it be sent in fermentation vat, adjust temperature and be 60-80 DEG C, pH value is 3.5-7, humidity is 55-80% fermentation process 20-30 days, and every 3 days turning and throwing once, after fermentation completes, it is added thereto to Folium Pini and Chinese medicine powder mix homogeneously, obtains just mixed thing；
(2) by Semen Maydis, Semen Tritici aestivi mixed grinding the sieve crossing 150-300 order, take undersize, by undersize and the clear water accounting for its weight 10-28%, stir, then through expanding treatment, obtain puffing material；Then in puffing material, add the water accounting for its weight 30-40% again, stir evenly form slurry；
(3) half of the slurry that step (2) prepares is taken; its first mixed thing obtained with step (1) is mixed; and it is stirred for mix homogeneously; it is sent to pelletize in comminutor again; and after granule is placed 1-3 days in the environment that temperature is 25-33 DEG C; it is placed in the environment that humidity is 80-90% again; controlling temperature is 1-5 DEG C of placement 1-3h; again by remaining half slurry even application at particle surface; adopting temperature to be 55-60 DEG C again, to dry be 5% to moisture, obtains rice field fish culture fish meal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610186077.1A CN105795162A (en) | 2016-03-25 | 2016-03-25 | Fish feed for culturing fish in rice field and preparation method of fish feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610186077.1A CN105795162A (en) | 2016-03-25 | 2016-03-25 | Fish feed for culturing fish in rice field and preparation method of fish feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105795162A true CN105795162A (en) | 2016-07-27 |
Family
ID=56453833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610186077.1A Pending CN105795162A (en) | 2016-03-25 | 2016-03-25 | Fish feed for culturing fish in rice field and preparation method of fish feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105795162A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719149A (en) * | 2016-12-15 | 2017-05-31 | 南宁学院 | A kind of method that use cold spring water cultivates Hehua fish in rice field |
| CN106942524A (en) * | 2017-03-22 | 2017-07-14 | 上林县山水牛畜牧业有限公司 | A kind of method that utilization cow dung prepares fish meal |
| CN107173641A (en) * | 2017-07-19 | 2017-09-19 | 合肥浦邦农业科技有限公司 | A kind of rice field fish culture fish meal and preparation method thereof |
| CN107183338A (en) * | 2017-05-11 | 2017-09-22 | 全椒县鮰鱼养殖专业合作社 | A kind of feed of Gai Shan Channel-catfish fish local flavors |
| CN108029875A (en) * | 2017-11-23 | 2018-05-15 | 定远县朱湾镇绿农养殖专业合作社 | A kind of fragrant pannage preparation method of low cost |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104522338A (en) * | 2014-11-21 | 2015-04-22 | 滁州市南谯区长江水产良种繁育场 | Fish feed prepared from biogas residue prepared from cow dung, and preparation method thereof |
| CN105029131A (en) * | 2015-09-15 | 2015-11-11 | 管天球 | Ecological fish feed |
-
2016
- 2016-03-25 CN CN201610186077.1A patent/CN105795162A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104522338A (en) * | 2014-11-21 | 2015-04-22 | 滁州市南谯区长江水产良种繁育场 | Fish feed prepared from biogas residue prepared from cow dung, and preparation method thereof |
| CN105029131A (en) * | 2015-09-15 | 2015-11-11 | 管天球 | Ecological fish feed |
Non-Patent Citations (2)
| Title |
|---|
| 吴心华、孙文华编: "《奶牛健康养殖技术》", 31 December 2013, 银川：阳光出版社 * |
| 蒋爱国等: "《高效生态养殖技术》", 30 April 2002, 南宁：广西科学技术出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719149A (en) * | 2016-12-15 | 2017-05-31 | 南宁学院 | A kind of method that use cold spring water cultivates Hehua fish in rice field |
| CN106942524A (en) * | 2017-03-22 | 2017-07-14 | 上林县山水牛畜牧业有限公司 | A kind of method that utilization cow dung prepares fish meal |
| CN107183338A (en) * | 2017-05-11 | 2017-09-22 | 全椒县鮰鱼养殖专业合作社 | A kind of feed of Gai Shan Channel-catfish fish local flavors |
| CN107173641A (en) * | 2017-07-19 | 2017-09-19 | 合肥浦邦农业科技有限公司 | A kind of rice field fish culture fish meal and preparation method thereof |
| CN108029875A (en) * | 2017-11-23 | 2018-05-15 | 定远县朱湾镇绿农养殖专业合作社 | A kind of fragrant pannage preparation method of low cost |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103704483B (en) | A kind of feed utilizing Chinese medicine dreg to ferment and preparation method thereof | |
| CN105767578A (en) | High-protein digestion promoting meat chicken feed and preparation method thereof | |
| CN105795162A (en) | Fish feed for culturing fish in rice field and preparation method of fish feed | |
| CN105393981B (en) | The fast well-fattened cultural method of the high damp-heat area beef cattle in south | |
| CN105075994A (en) | Breeding method for earthworms | |
| CN105166459A (en) | Pig feed | |
| CN101861953B (en) | Chinese medical herb composition for feed, feed additive, feed and preparation method | |
| CN105192327A (en) | Preparation method of growing and fattening pig biological feed | |
| CN105767516A (en) | High-protein summer heat relieving and stomach strengthening traditional Chinese medicinal composition fermented pig feed and preparation method thereof | |
| CN105962189A (en) | Compound fruit and vegetable enzyme and preparation method thereof | |
| CN110037166A (en) | A kind of preparation method of Procambius clarkii Chinese herbal medicine composite fermented feed | |
| CN105941824A (en) | Traditional Chinese medicine microbial probiotic original liquid, feed, fertilizer and pesticide, and preparation methods thereof | |
| CN106962681A (en) | A kind of feed of grass carp and preparation method thereof | |
| CN105410441A (en) | Probiotics-containing broiler compound feed and preparation method thereof | |
| CN105265804A (en) | Preparation method of feed for improving milk yield of cows | |
| CN106212371A (en) | A kind of cultural method of bar horse perfume (or spice) boar | |
| CN104497094B (en) | Processing method based on comprehensive utilization of stems and roots of cucurbitaceae melon plants | |
| CN106561565A (en) | Feeding method for black goats | |
| CN101861912B (en) | Feed Chinese medical herb composition, feed additive, feed and preparation method | |
| CN105753581A (en) | Special fertilizer for snakegourd fruits | |
| CN106306431A (en) | Traditional Chinese medicine health-care feed additive facilitating growth promotion of beef cattle and mutton sheep | |
| CN109006179A (en) | A kind of production method of mushroom cultivation substrate | |
| CN106616001A (en) | Novel tilapia feed produced from cassava lees through microbial fermentation and preparation method of tilapia feed | |
| CN105815595A (en) | Green high-protein chicken feed formula for improving immunity of chicken body | |
| CN106260575A (en) | A kind of Ecocyclic cultivation, implantation methods |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160727 |
|
| RJ01 | Rejection of invention patent application after publication |