CN103704483B - A kind of feed utilizing Chinese medicine dreg to ferment and preparation method thereof - Google Patents
A kind of feed utilizing Chinese medicine dreg to ferment and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of feed utilizing Chinese medicine dreg to ferment and preparation method thereof, described feed is fermented through fungi bacterium liquid by Chinese medicine dreg, stalk and wheat bran, and it is obtained to add probiotic microorganisms microbial inoculum, in described feed: Chinese medicine dreg 80-120 part, stalk 40-100 part, wheat bran 10-40 part, fungi bacterium liquid 1-10 part, probiotic microorganisms microbial inoculum 10-50 part.In feed provided by the invention, crude protein content is high, can promote the growth of domestic birds and animals, have obvious effect, and effect is all better than prior art to the weightening finish of domestic birds and animals and the raising of feed efficiency.
Description
Technical field
The present invention relates to a kind of fermented feed, be specifically related to a kind of feed utilizing Chinese medicine dreg to ferment and preparation method thereof.
Background technology
Chinese herbal medicine is the important component part of motherland's traditional medicine, and the exploitation of new formulation and the standardization of Chinese patent drug are produced, and Chinese herbal medicine is progressively gone to the world.Simultaneously in recent years along with the understanding intensification of people to chemical drugs toxic and side effect and the progressively formation of concept of modern health, the tcm product with long history and unique curative effect receives much concern with its comprehensive strong, low toxicity, low-residual feature, and domestic and international medical market also just constantly increases for the demand of Chinese medicine.But China's Chinese Medicine Industry produces the dregs of a decoction every year has reached 3,000 ten thousand tons, it is reported that most enterprise selects landfill or incinerating method to process, this original, simple processing mode brings great waste not only to enterprise self, especially all causes great hidden danger to environment and public health.
On the other hand, the safety problem of fowl, livestock products especially attracts people's attention.Feed addictive such as antibiotic, somatotropin, pest repellant, hormone, flavor enhancement, modifying agent, the anticorrisive agent etc. of a large amount of Prof. Du Yucang generally use in feed, and cause the residue of veterinary drug in animal body.And the feed addictive of Chinese medicine type has antibacterial, growth promotion with it, effectively improves animal immunizing power, the less residual feature such as in animal body, show all the more the importance to animal health and advantage.But because Chinese herbal medicine resource is limited, Long-Time Service Chinese herbal feed additive, must cause people beast to strive the situation of medicine, the present situation that aggravation Chinese herbal medicine resource lacks.
For Zhuzhou Qianjin Pharmacy Co., Ltd, it is main that the said firm produces the Chinese patent medicine preparation such as FUKE QIANJIN PIAN, ' Qianjin ' capsule to treat ganopathy, wherein:
FUKE QIANJIN PIAN, as CN98112696.0(Authorization Notice No. CN1078079C), disclose the preparation method of sugar coated tablet or Film coated tablets, be specially: the Radix Angelicae Sinensis of 9 weight portions, Radix Codonopsis, Herba Andrographitis, in pulverizer, be ground into the fine powder of more than 100 orders, leave and take for subsequent use according to the powder rate that obtains of at least 93.3%; Choose the cherokee rose root of the one side pin of 9 weight portions and 16 weight portions, reticulate millettia, Chinese mahonia and very heavyly pull out traditional Chinese medicine of the five flavours, they are placed in multi-function extractor, add hot water and extract twice by the every 2 hours methods extracted once, cross leaching liquid, this extract is condensed in Three-effect concentration device proportion is 1.08(80 DEG C simultaneously) medicinal extract for subsequent use; By fine powder and medicinal extract mixed pelletization, and be made into particle by boiling drier, after compressing tablet, enrobing processes, make sugar coated tablet or Film coated tablets.
' Qianjin ' capsule to treat ganopathy, if CN99115683.8(Authorization Notice No. is CN1158087C), CN99115682.X(Authorization Notice No. is CN1170549C), wherein the preparation method of CN99115683.8 is: the Radix Angelicae Sinensis one of the Herba Andrographitis of 9% and 9% is reinstated the ethanol of 85% concentration through heating reflux method extraction filtration, reclaim ethanol, and filtrate is condensed into cream clearly; The one side pin poach of the leatherleaf mahonia of 16% and 9% is extracted, after filtering de-slag, filtrate is condensed into cream clearly; 16% very heavyly to pull out, the cherokee rose root of 16%, the reticulate millettia of 16% and 9% Radix Codonopsis poach extract, filter de-slag and get filtrate, and the filter residue of filter residue and Herba Andrographitis and Radix Angelicae Sinensis is merged, after poach extracts again, filtrate after twice gained filter is merged, and be condensed into cream clearly, merge three kinds of clear cream, mixing, spraying dry, granulation, fillingly namely obtain capsule; The preparation method of CN99115682.X is: the incorporation of Radix Angelicae Sinensis, Radix Codonopsis, Herba Andrographitis and one side pin is respectively 9% of medicinal material total amount; Cherokee rose root, reticulate millettia, leatherleaf mahonia and the very heavy incorporation pulled out are respectively 16% of medicinal material total amount; Its preparation method is: extracted by Radix Angelicae Sinensis alcohol percolation method and make cream clearly; Herba Andrographitis ethanol refluxing process is extracted and makes cream clearly; Leatherleaf mahonia, one side pin poach are extracted twice, after filtering the merging of both filtrates is made cream clearly; By very heavyly pulling out, cherokee rose root, reticulate millettia and Radix Codonopsis poach extract and once filter afterwards, by the dregs of a decoction poach together with the Radix Angelicae Sinensis dregs of a decoction, the Herba Andrographitis dregs of a decoction after filtration, filter, merging filtrate also makes cream clearly, merge above-mentioned four kinds of clear cream, mixing, spraying dry, granulation, filling, obtain ' Qianjin ' capsule to treat ganopathy.
Chinese medicine dreg is as plant discarded object, and still containing plurality of active ingredients such as cellulose, protein, polysaccharide, left drug compositions, making full use of discarded Chinese medicine dreg resource has important realistic meaning: one is twice laid, protection of the environment; Two is the expenses of saving factory processes discarded object; Three is make high value added product with Chinese medicine dreg, and existing resource is maximized the use.
At present, the recycling of Chinese medicine dreg is more and more subject to the attention of medicine enterprise and various circles of society, and the direction of exploitation is also enriched gradually, such as, the dregs of a decoction are developed as animal feed, compost, fermentation methane production, make active carbon etc.
Chinese medicine dreg solid state fermentation produce protein feed (Wang Bing etc., Chinese medicine dreg solid state fermentation produce protein feed, food and biotechnology journal, in July, 2007,26 volumes (4): 77-82) disclose with whiterot fungi to the dregs of a decoction ferment method.
CN91104240.7(publication number is CN1059068A) disclose the method for Chinese medicine dreg culturing edible fungus mushroom, pass through breeding strain, the preparation of planting material, inoculation, mycelia are cultivated, the process procedures such as management of producing mushroom, cultivate edible fungus cluster, the method mainly dregs of a decoction directly cultivates bacterium mushroom, does not relate to the method for dregs of a decoction fermentation.
CN201210438591.1(publication number is CN102919513A) disclose Chinese medicine dreg, fungal fermented filtrate, maize straw, wheat bran fermentation feed, wherein fungal fermented filtrate is lucidum strain, flat mushroom, pleurotus eryngii.
Chinese medicine dreg, except above-mentioned report, is combine for new approach with other Chinese medicine or edible mushrooms etc. mostly.
But namely the key issue of the recycling dregs of a decoction simplifies development technology and reduction development cost at present, therefore works out and is easy to operation, the new medicine dregs of a decoction fermentation technique of convenient production is the task of top priority.
Summary of the invention
The object of this invention is to provide a kind of feed utilizing Chinese medicine dreg to ferment.
A kind of feed utilizing Chinese medicine dreg to ferment provided by the invention, described feed is fermented through fungi bacterium liquid by Chinese medicine dreg, stalk and wheat bran, and it is obtained to add probiotic microorganisms microbial inoculum.
Concrete, in described feed: Chinese medicine dreg 80-120 part, stalk 40-100 part, wheat bran 10-40 part, fungi bacterium liquid 1-10 part, probiotic microorganisms microbial inoculum 10-50 part.
Preferably, in described feed: Chinese medicine dreg 100-120 part, stalk 50-100 part, wheat bran 10-40 part, fungi bacterium liquid 1.5-8.0 part, probiotic microorganisms microbial inoculum 15-40 part.
Further preferably, in described feed: Chinese medicine dreg 100-105 part, stalk 70-80 part, wheat bran 17-20 part, fungi bacterium liquid 3.0-4.0 part, probiotic microorganisms microbial inoculum 23-30 part.
Further preferred, in described feed: Chinese medicine dreg 100 parts, stalk 80 parts, 20 parts, wheat bran, fungi bacterium liquid 4.0 parts, probiotic microorganisms microbial inoculum 30 parts.
In above-mentioned feed:
Described weight portion can be the known unit of weights of field of medicaments such as μ g, mg, g, kg, also can be its multiple, as 1/10,1/100,10 times, 100 times etc.
Described Chinese medicine dreg be very heavyly to pull out, cherokee rose root, reticulate millettia, one side pin, leatherleaf mahonia, Herba Andrographitis, Radix Angelicae Sinensis, remaining residue after one or more traditional Chinese medicine extraction in Radix Codonopsis;
Preferably, the described dregs of a decoction are remaining residue after the traditional Chinese medicine extraction of following weight portion: Radix Angelicae Sinensis 8-12 part, Radix Codonopsis 8-12 part, Herba Andrographitis 8-12 part, one side pin 8-12 part, leatherleaf mahonia 12-18 part, very heavyly pull out 12-18 part, cherokee rose root 12-18 part, reticulate millettia 12-18 part;
Further preferably, the described dregs of a decoction are the pharmacy residue of FUKE QIANJIN PIAN or ' Qianjin ' capsule to treat ganopathy.
Described stalk is rice straw, maize straw or wheat stalk, is preferably rice straw.
Described fungi bacterium liquid is black fungus, rainbow conk or Hericium erinaceus, is preferably Hericium erinaceus.
Described fungi bacterium liquid, prepared by following methods:
1) preparation of fungi seed liquor: get black fungus flat board, rainbow conk is dull and stereotyped or Hericium erinaceus is dull and stereotyped, take bacterial classification block drop into fresh PDA culture medium with aseptic inoculation shovel, 120-150rpm rotating speed cultivates 60-72h 25 DEG C of concussions, namely makes fungi seed liquor;
2) preparation of fungi bacterium liquid: join in PDA fluid nutrient medium by fungi seed liquor according to the inoculum concentration of 0.3%-0.8%, keeps temperature 25 DEG C, the condition of culture of 120-150rpm rotating speed, cultivates 2-5 days, obtains fungi bacterium liquid.
Described PDA culture medium: potato leaching liquid 1.0L, glucose 20.0g, agar 15.0g, pH value nature, in 121 DEG C of sterilizing 20min.
Described probiotic microorganisms microbial inoculum is Bifidobacterium probiotics, can directly by commercially available purchase.
Concrete, described feed is prepared by following methods:
1) collect Chinese medicine dreg, stalk and wheat bran, be ground into particle respectively, particle diameter is 0.4-0.8cm;
2) dregs of a decoction moist heat sterilization crushed 121 DEG C, 30-40min;
3) dregs of a decoction after being exposed to the sun mix according to the above ratio with stalk, wheat bran, and agitation as appropriate is even;
4) the fungi bacterium liquid of sprayed-out recipe amount, mixes rear 22-28 DEG C heap fermentation, turning in 4-7 days, fermentation 30-40 days;
5) material after fermentation suitably reduces moisture to 18-20%, adds the probiotic microorganisms microbial inoculum of formula ratio, mixing, dry, to obtain final product.
In said method:
In the sweat of step 4), ensure good ventilation, when material gradually dehydration time note adding water and keep fermentation materials moisture to 55-60%;
Drying in described step 5) is air-dry or dries, and notices that temperature is at 45-50 DEG C, when moisture is down to 10-15%, packs.
Present invention also offers the preparation method of above-mentioned feed, the method comprises the following steps:
1) collect Chinese medicine dreg, stalk and wheat bran, be ground into particle respectively, particle diameter is 0.4-0.8cm;
2) dregs of a decoction moist heat sterilization crushed 121 DEG C, 30-40min;
3) dregs of a decoction after being exposed to the sun mix according to the above ratio with stalk, wheat bran, and agitation as appropriate is even;
4) the fungi bacterium liquid of sprayed-out recipe amount, mixes rear 22-28 DEG C heap fermentation, turning in 4-7 days, fermentation 30-40 days;
5) material after fermentation suitably reduces moisture to 18-20%, adds the probiotic microorganisms microbial inoculum of formula ratio, mixing, dry, to obtain final product.
In said method:
In the sweat of step 4), ensure good ventilation, when material gradually dehydration time note adding water and keep fermentation materials moisture to 55-60%;
Drying in described step 5) is air-dry or dries, and notices that temperature is at 45-50 DEG C, when moisture is down to 10-15%, packs.
Present invention also offers the application of above-mentioned feed in raise poultry domestic animal.
The feed of Chinese medicine dreg fermentation provided by the invention has the following advantages:
First, the present invention adopts the edible fungus fermented tcm dregs of a decoction, stalk and wheat bran, has fully ensured the security of feedstuff, and selects the degradation capability of bacterial classification strong, and catabolite is beneficial to domestic birds and animals and eats;
Secondly, mainly adopt Chinese medicine dreg and stalk to be raw material, take full advantage of Chinese medicine dreg and these two kinds of industry of stalk, agricultural wastes, both saved the expense of the dregs of a decoction, stalk reprocessing, and again reduced Feed Manufacturing cost;
3rd, technical scheme method provided by the invention is simple, can greatly meet industrialization demand.
Moreover, add part probio, more can strengthen effect of this feed, the effect making it have diseases prevention, strengthen domestic birds and animals immunity, improve domestic birds and animals rate of body weight gain, with take general feeds equivalent weight domestic birds and animals compared with, it is more obvious that body weight increases effect, and the transformation efficiency of feed is higher.Meanwhile also effectively alleviate the problem of Chinese herbal feed raw material resources shortage.
3, in feed provided by the invention, crude protein content is high, can promote the growth of domestic birds and animals, have obvious effect, and effect is all better than prior art to the weightening finish of domestic birds and animals and the raising of feed efficiency.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Black fungus is dull and stereotyped, rainbow conk is dull and stereotyped or Hericium erinaceus is dull and stereotyped can by commercially available direct purchase.
The method for making of fungi bacterium liquid is:
Method for making one:
1) preparation of fungi seed liquor: get black fungus flat board, rainbow conk is dull and stereotyped or Hericium erinaceus is dull and stereotyped, with the bacterial classification block of 0.3cm × 0.3cm for unit, black fungus flat board, rainbow conk flat board or dull and stereotyped 10 pieces of Hericium erinaceus is taken respectively with aseptic inoculation shovel, drop into 400mlPDA culture medium, 120rpm rotating speed cultivates 60h 25 DEG C of concussions, namely makes fungi seed liquor;
2) according to 0.3% inoculum concentration, fungi seed liquor is joined in PDA fluid nutrient medium, is incubated 25 DEG C, 120rpm shakes cultivation 2 days, obtain fungi bacterium liquid.
Method for making two:
1) preparation of fungi seed liquor: get black fungus flat board, rainbow conk is dull and stereotyped or Hericium erinaceus is dull and stereotyped, with the bacterial classification block of 0.3cm × 0.3cm for unit, black fungus flat board, rainbow conk flat board or dull and stereotyped 5 pieces of Hericium erinaceus is taken respectively with aseptic inoculation shovel, drop into 350mlPDA culture medium, 135rpm rotating speed cultivates 66h 25 DEG C of concussions, namely makes fungi seed liquor;
2) according to 0.5% inoculum concentration, fungi seed liquor is joined in PDA fluid nutrient medium, is incubated 25 DEG C, 135rpm shakes cultivation 4 days, obtain fungi bacterium liquid.
Method for making three:
1) preparation of fungi seed liquor: get black fungus flat board, rainbow conk is dull and stereotyped or Hericium erinaceus is dull and stereotyped, with the bacterial classification block of 0.3cm × 0.3cm for unit, black fungus flat board, rainbow conk flat board or dull and stereotyped 2 pieces of Hericium erinaceus is taken respectively with aseptic inoculation shovel, drop into 200mlPDA culture medium, 150rpm rotating speed cultivates 72h 25 DEG C of concussions, namely makes fungi seed liquor;
2) according to 0.8% inoculum concentration, fungi seed liquor is joined in PDA fluid nutrient medium, is incubated 25 DEG C, 150rpm shakes cultivation 5 days, obtain fungi bacterium liquid.
Described PDA culture medium: potato leaching liquid 1.0L, glucose 20.0g, agar 15.0g, pH value nature, in 121 DEG C of sterilizing 20min.
Described probiotic microorganisms microbial inoculum is Bifidobacterium probiotics, buys producer: Borrow Di Muhan (Weifang) bio tech ltd, and trade name is health care probio-Bifidobacterium.
Embodiment 1: utilize the feed that Chinese medicine dreg ferments
1, raw material: Chinese medicine dreg (be the pharmacy waste residue of FUKE QIANJIN PIAN, its preparation method is as CN1078079C) 100kg, rice straw 50kg, wheat bran 12.5kg, black fungus fungi bacterium liquid (fungi method for making one) 1.625kg, probiotic microorganisms microbial inoculum 16.25kg.
2, preparation method:
(1) collect Chinese medicine dreg, rice straw, wheat bran, be ground into particle respectively, particle diameter is 0.4cm;
(2) dregs of a decoction moist heat sterilization crushed 121 DEG C, 30min;
(3) the Chinese medicine dreg 100kg, the rice straw 50kg that take formula ratio mix with wheat bran 12.5kg, and agitation as appropriate is even;
(4) spray with fungi bacterium liquid 1.625kg, mix rear maintenance temperature 22 DEG C, heap fermentation 30 days, turning in 4 days once;
(5) ensure good ventilation in said process, when material gradually dehydration time note adding water and keep fermentation materials moisture to 55%.
(6) material after fermentation suitably reduces moisture to 18%, adds 16.25kg probiotic microorganisms microbial inoculum, mixing;
(7) by air-dry for above-mentioned feed or oven dry, notice that temperature keeps about 45 DEG C, moisture is down to 10%, packs, and to obtain final product.
Embodiment 2: utilize the feed that Chinese medicine dreg ferments
1, raw material: Chinese medicine dreg (be the pharmacy waste residue of FUKE QIANJIN PIAN, its preparation method is as CN1078079C) 110kg, rice straw 88kg, wheat bran 22kg, rainbow conk fungi bacterium liquid (fungi method for making two) 4.4kg, probiotic microorganisms microbial inoculum 26.4kg.
2, preparation method:
(1) collect Chinese medicine dreg, rice straw, wheat bran, be ground into particle respectively, particle diameter is 0.5cm;
(2) dregs of a decoction moist heat sterilization crushed 121 DEG C, 35min;
(3) take formula ratio Chinese medicine dreg 110kg, rice straw 88kg mixes with wheat bran 22kg, agitation as appropriate is even;
(4) spray fungi bacterium liquid 4.4kg, mix rear maintenance temperature 28 DEG C, heap fermentation 40 days, turning in 6 days once;
(5) ensure good ventilation in said process, when material gradually dehydration time note adding water and keep fermentation materials moisture to 60%;
(6) material after fermentation suitably reduces moisture to 20%, adds 26.4kg probiotic microorganisms microbial inoculum, mixing;
(7) by air-dry for above-mentioned feed or oven dry, notice that temperature keeps about 45 DEG C, moisture is down to 10%, packs, and to obtain final product.
Embodiment 3: utilize the feed that Chinese medicine dreg ferments
1, raw material: Chinese medicine dreg (be the pharmacy waste residue of ' Qianjin ' capsule to treat ganopathy, its preparation method is as CN1158087C) 120kg, rice straw 100kg, wheat bran 40kg, rainbow conk fungi bacterium liquid (fungi method for making three) 7.8kg, probiotic microorganisms microbial inoculum 39kg.
2, preparation method:
(1) collect Chinese medicine dreg, rice straw, wheat bran, be ground into particle respectively, particle diameter is 0.8cm;
(2) dregs of a decoction moist heat sterilization crushed 121 DEG C, 40min;
(3) take formula ratio Chinese medicine dreg 120kg, rice straw 100kg mixes with wheat bran 40kg, agitation as appropriate is even;
(4) spray fungi bacterium liquid 7.8kg, mix rear maintenance temperature 25 DEG C, heap fermentation 35 days, turning in 7 days once;
(5) ensure good ventilation in said process, when material gradually dehydration time note adding water and keep fermentation materials moisture to 58%.
(6) material after fermentation suitably reduces moisture to 18%, adds 39kg probiotic microorganisms microbial inoculum, mixing;
(7) by air-dry for above-mentioned feed or oven dry, notice that temperature keeps about 45 DEG C, moisture is down to 10%, packs, and to obtain final product.
Embodiment 4: utilize the feed that Chinese medicine dreg ferments
1, raw material: Chinese medicine dreg (be the pharmacy waste residue of ' Qianjin ' capsule to treat ganopathy, its preparation method is as CN1158087C) 105kg, maize straw 63kg, wheat bran 10.5kg, Hericium erinaceus fungi bacterium liquid (fungi method for making one) 1.8kg, probiotic microorganisms microbial inoculum 18.4kg.
2, preparation method:
(1) collect Chinese medicine dreg, maize straw, wheat bran, be ground into particle respectively, particle diameter is 0.6cm;
(2) dregs of a decoction moist heat sterilization crushed 121 DEG C, 35min;
(3) take formula ratio Chinese medicine dreg 105kg, maize straw 63kg mixes with wheat bran 10.5kg, agitation as appropriate is even;
(4) spray with fungi bacterium liquid 1.8kg, mix rear maintenance 22 DEG C, heap fermentation 30 days, turning in 4 days once;
(5) ensure good ventilation in said process, when material gradually dehydration time note adding water and keep fermentation materials moisture to 55%;
(6) material after fermentation suitably reduces moisture to 18%, adds 18.4kg probiotic microorganisms microbial inoculum, mixing;
(7) by air-dry for above-mentioned feed or oven dry, notice that temperature keeps about 45 DEG C, moisture is down to 15%, packs, and to obtain final product.
Embodiment 5: utilize the feed that Chinese medicine dreg ferments
1, raw material: Chinese medicine dreg (be the pharmacy waste residue of ' Qianjin ' capsule to treat ganopathy, its preparation method is as CN1170549C) 105kg, wheat stalk 75kg, wheat bran 17kg, black fungus fungi bacterium liquid (fungi method for making two) 3.0kg, probiotic microorganisms microbial inoculum 23.64kg.
2, preparation method
(1) collect Chinese medicine dreg, wheat stalk, wheat bran, be ground into particle respectively, particle diameter is 0.4cm;
(2) dregs of a decoction moist heat sterilization crushed 121 DEG C, 30min;
(3) take formula ratio Chinese medicine dreg 105kg, wheat stalk 75kg mixes with wheat bran 17kg, agitation as appropriate is even;
(4) spray with fungi bacterium liquid 3.0kg, mix rear maintenance 28 DEG C, heap fermentation 40 days, turning in 6 days once;
(5) ensure good ventilation in said process, when material gradually dehydration time note adding water and keep fermentation materials moisture to 60%;
(6) material after fermentation suitably reduces moisture to 18%, adds 23.64kg probiotic microorganisms microbial inoculum, mixing;
(7) by air-dry for above-mentioned feed or oven dry, notice that temperature keeps about 45 DEG C, moisture is down to 10%, packs, and to obtain final product.
Embodiment 6: utilize the feed that Chinese medicine dreg ferments
1, raw material: Chinese medicine dreg (be the pharmacy waste residue of ' Qianjin ' capsule to treat ganopathy, its preparation method is as CN1170549C) 100kg, rice straw 80kg, wheat bran 20kg, Hericium erinaceus fungi bacterium liquid (fungi method for making three) 4.0kg, probiotic microorganisms microbial inoculum 30kg.
2, preparation method:
(1) with embodiment 1;
(2) with embodiment 1;
(3) cruelly take formula ratio Chinese medicine dreg 100kg, rice straw 80kg mixes with wheat bran 20kg, agitation as appropriate is even;
(4) spray fungi bacterium liquid 4.0kg, mix rear maintenance 25 DEG C, heap fermentation 35 days, turning in 7 days once;
(5) ensure good ventilation in said process, when material gradually dehydration time note adding water and keep fermentation materials moisture to 55%.
(6) material after fermentation suitably reduces moisture to 18%, adds 30kg probiotic microorganisms microbial inoculum, mixing;
(7) by air-dry for above-mentioned feed or oven dry, notice that temperature keeps about 45 DEG C, moisture is down to 15%, packs, and to obtain final product.
Comparative example 1:
Reference Wang Bing etc. produce the method (in July, 2007,26 volumes (4): 77-82) of protein feed at the Chinese medicine dreg solid state fermentation that " food and biotechnology journal " is delivered, and ferment to the dregs of a decoction with whiterot fungi, concrete grammar is:
1, the dregs of a decoction: adopt the dregs of a decoction identical with embodiment 6.
2, culture medium: PDA culture medium: potato leaching liquid 1.0L, glucose 20.0g, agar 15.0g, pH value nature, in 121 DEG C of sterilizing 20min, for Spawn incubation.
Solid-state fermentation culture medium: be unique culture matrix with Chinese medicine dreg, add a certain amount of moisture, Diammonium phosphate (DAP) and trace element, be mixed with for investigating glucose addition, Diammonium phosphate (DAP) addition, material water quality ratio, initial pH value, fermentation time to the different culture media of the impact of the true albumen of tunning and crude fiber content.
3, after whiterot fungi being activated for 3 generations on PDA slant medium, make certain density spore suspension, be then inoculated in solid-state fermentation culture medium, after 35-39 DEG C of static gas wave refrigerator certain hour, 55-65 DEG C of oven dry.
Comparative example 2:
Be CN102919513A with reference to CN201210438591.1(publication number) the true albumen growth rate of embodiment 1(be 53.2%), concrete grammar is:
1, Chinese medicine slag, rice straw and wheat bran are broken into the particle that particle diameter is 0.5-1cm;
2, above-mentioned Chinese medicine slag, maize straw and the wheat bran crushed is exposed to the sun 15 hours under outdoor solar light, described Chinese medicine slag is the mixture of pharmacy waste residue according to the weight ratio of 1:1 of Yinqiao detoxification and the Pu blue sulfathiazole in ground two kinds of Chinese patent drugs, and wherein the composition of Yinqiao detoxification comprises honeysuckle flower, great burdock achene, the capsule of weeping forsythia, the leaf of bamboo, Radix Glycyrrhizae and Fermented Soybean; The composition of the blue sulfathiazole in ground, Pu comprises dandelion, Chinese violet, Radix Isatidis and the root of large-flowered skullcap, stores for future use after going out pharmaceutical factory through dehydration;
3, the proportioning of the Chinese medicine dreg tanned by the sun with rice straw, wheat bran reference example 6 mixed, add water and high-temperature sterilization, high-temperature sterilization condition is: sterilizing 120min under 121 DEG C of conditions;
4, the compound inoculation fermentation liquid after above-mentioned sterilizing is stirred, zymotic fluid be flat mushroom strain be seeded to add in rich PDA fluid nutrient medium in 28 DEG C, 150r/min cultivates 4 days, inoculum concentration is 3%;
5, drying is taken out after above-mentioned postvaccinal fermentation material being cultivated 7 days in 28 DEG C.
Experimental example 1: the detection of feed crude protein
1, principle: according to the nitrogen content in Kjeldahl nitrogen determination sample, first make nitrogen substance change into ammonium sulfate with sulfuric acid, add highly basic and ammonia is overflowed, absorbs with boric acid, then uses acidometric titration, be nitrogen content.
2, concrete detection method:
Divide to 200g by sample quartering, be then crushed to all by 0.90mm hole sizer (20 order), it is for subsequent use that mixing is loaded on airtight container.
From ready sample, take 1g sample be placed in kjeldahl flask, add the mixed catalyst (0.4g copper sulphate, 6g potassium sulfate) of 6.4g and 12ml sulfuric acid and 2 beades, be heated to sample coking, treat lather collapse, keep 360-410 DEG C, to the transparent blue-green of solution, then continue heating 2h, disappear and boil completely.
Disappear and boil liquid cooling and but proceed to 100ml volumetric flask afterwards, add water to scale, shake up.Link semimicro distilling apparatus, immerses 20ml boric acid and the conical flask containing 2 mixed indicators (methyl red and bromocresol green solution) by condenser pipe end.Disappear and boil liquid and pour distilling apparatus into, after sealing, start distillation.
Absorbing liquid after distillation uses the titration of 0.1M/L hydrochloric acid standard solution immediately, and it is titration end-point that solution becomes bois de rose by blue-green.By nitrogen content and the protein content of the volume computing sample of titration hydrochloric acid.
3, measurement result: in table 1
Table 1: crude protein content of feed measures
Table 1 result shows: in the product feed of comparative example 1,2, protein content is lower than 19%, and crude protein growth rate is lower than 43%, and in embodiment of the present invention 1-6, the protein content of finished product feedstuff is more than 19.8%, and notably up to 22.8%, and crude protein growth rate is all higher than 45%.
Result shows: the crude protein content of feed of Chinese medicine dreg fermentation of the present invention is high, is better than prior art.
Experimental example 2: raising experiment
1, place is implemented: home poultry raising base, a thousand pieces of gold medicine company dining room, Zhuzhou
2, material is implemented: respectively according to fermented feed prepared by the embodiment of the present invention 1, embodiment 6 method, the feed prepared according to comparative example 1 and comparative example 2 method, Broiler chicks.
3, grouping is implemented: test establishes four groups, is respectively A1: the Chinese medicine slag fermented feed group prepared according to embodiment 1; A2: the Chinese medicine slag fermented feed group prepared according to embodiment 6; B1: the fermented feed group prepared according to comparative example 1 method; B2: the fermented feed group prepared according to comparative example 2 method; C: normal diet group.Each group comprises 25 age in days Broiler chicks 20 (body weight is 810 ± 20g).
4, feeding and management: each group 25 age in days Broiler chicks is except feed uses difference, and other management are consistent.Broiler chicks body weight and feed efficiency is respectively organized when recording 35 ages in days, 45 age in days respectively.
Growth promotion efficiency (%)=(processed group body weight-control group body weight)/control group body weight × 100%;
Feed efficiency (%)=(when test is measured body weight-original body mass)/throw feed gross weight × 100%.
5, experimental result: in table 2
Table 2: Broiler chicks body weight during different days
Note: a: compared to A1 group, p<0.05, has significant difference; B: compared to A2 group, p<0.05, has significant difference; C: compared to B1 group, p<0.05, has significant difference; D: compared to B2 group, p<0.05, has significant difference; The calculating of growth promotion rate all with C group for control group.
Table 3: Broiler chicks feed efficiency during different days
Note: a: compared to A1 group, p<0.05, has significant difference; B: compared to A2 group, p<0.05, has significant difference; C: compared to B1 group, p<0.05, has significant difference; D: compared to B2 group, p<0.05, has significant difference
Table 2,3 result displays:
Aspect is affected on Broiler chicks body weight: when 35 age in days, each group of effect giving special feed is all better than C group; After 45 ages in days, compared with B1, B2 group, the Broiler chicks body weight of A1 and A2 group has remarkable growth, and growth promotion efficiency obviously increases.
Feed efficiency aspect: A1 and A2 group is all higher than B1, B2, C group.
Equally, other embodiments of the present invention and proportioning are tested, effect and embodiment 1,6 similar, wherein crude protein content and embodiment 6 close, effect is identical with embodiment 6.
Result shows: use forage feed Broiler chicks of the present invention, the feed that daily gain is prepared higher than method for making in normal diet and comparative example 1, comparative example 2, can accelerate chick growth, reach 11.8% compared to the growth promotion rate of normal diet; Through comparing, the transformation efficiency of feed of the present invention, also apparently higher than comparative example feed and normal diet, has significant difference.Find additionally by observation, the regularity of the overall chicken of Chinese medicine dreg feed group of the present invention is relative to comparative example 1,2 better.
Although above with general explanation, detailed description of the invention and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (9)
1. the feed utilizing Chinese medicine dreg to ferment, it is characterized in that, described feed is by Chinese medicine dreg, stalk and wheat bran ferment through fungi bacterium liquid, and it is obtained to add probiotic microorganisms microbial inoculum, in described feed: Chinese medicine dreg 80-120 part, stalk 40-100 part, wheat bran 10-40 part, fungi bacterium liquid 1-10 part, probiotic microorganisms microbial inoculum 10-50 part, the described dregs of a decoction are remaining residue after following traditional Chinese medicine extraction: Radix Angelicae Sinensis 8-12 part, Radix Codonopsis 8-12 part, Herba Andrographitis 8-12 part, one side pin 8-12 part, leatherleaf mahonia 12-18 part, very heavyly pull out 12-18 part, cherokee rose root 12-18 part, reticulate millettia 12-18 part, described fungi is black fungus, rainbow conk or Hericium erinaceus, described probiotic microorganisms microbial inoculum is Bifidobacterium probiotics, and described feed is prepared by following methods:
1) collect Chinese medicine dreg, stalk and wheat bran, be ground into particle respectively, particle diameter is 0.4-0.8cm;
2) dregs of a decoction moist heat sterilization crushed 121 DEG C, 30-40min;
3) dregs of a decoction mix by proportioning with stalk, wheat bran, and agitation as appropriate is even;
4) the fungi bacterium liquid of sprayed-out recipe amount, mixes rear 22-28 DEG C heap fermentation, and once, ferment 30-40 days in turning in 4-7 days;
5) material after fermentation suitably reduces moisture to 18-20%, adds the probiotic microorganisms microbial inoculum of formula ratio, mixing, dry, to obtain final product.
2. feed according to claim 1, is characterized in that, in described feed: Chinese medicine dreg 100-120 part, stalk 50-100 part, wheat bran 10-40 part, fungi bacterium liquid 1.5-8.0 part, probiotic microorganisms microbial inoculum 15-40 part.
3. feed according to claim 1, is characterized in that, in described feed: Chinese medicine dreg 100-105 part, stalk 70-80 part, wheat bran 17-20 part, fungi bacterium liquid 3.0-4.0 part, probiotic microorganisms microbial inoculum 23-30 part.
4. feed according to claim 1, is characterized in that, in described feed: Chinese medicine dreg 100 parts, stalk 80 parts, 20 parts, wheat bran, fungi bacterium liquid 4.0 parts, probiotic microorganisms microbial inoculum 30 parts.
5. feed according to claim 1, is characterized in that, the described dregs of a decoction are the pharmacy residue of FUKE QIANJIN PIAN or ' Qianjin ' capsule to treat ganopathy.
6. the feed according to any one of claim 1-4, is characterized in that, described fungi is Hericium erinaceus.
7. feed according to claim 1, is characterized in that, described fungi bacterium liquid, is prepared by following methods:
1) preparation of fungi seed liquor: get black fungus flat board, rainbow conk is dull and stereotyped or Hericium erinaceus is dull and stereotyped, take bacterial classification block and drop into fresh PDA culture medium, 120-150rpm rotating speed cultivates 60-72h 25 DEG C of concussions, namely makes fungi seed liquor;
2) preparation of fungi bacterium liquid: join in PDA fluid nutrient medium by fungi seed liquor according to the inoculum concentration of 0.3%-0.8%, keeps temperature 25 DEG C, the condition of culture of 120-150rpm rotating speed, cultivates 2-5 days, obtains fungi bacterium liquid.
8. prepare a method for feed described in any one of claim 1-7, it is characterized in that, the method comprises the following steps:
1) collect Chinese medicine dreg, stalk and wheat bran, be ground into particle respectively, particle diameter is 0.4-0.8cm;
2) dregs of a decoction moist heat sterilization crushed 121 DEG C, 30-40min;
3) dregs of a decoction mix by proportioning with stalk, wheat bran, and agitation as appropriate is even;
4) the fungi bacterium liquid of sprayed-out recipe amount, mixes rear 22-28 DEG C heap fermentation, and once, ferment 30-40 days in turning in 4-7 days;
5) material after fermentation suitably reduces moisture to 18-20%, adds the probiotic microorganisms microbial inoculum of formula ratio, mixing, dry, to obtain final product.
9. the application of the feed described in any one of claim 1-7 in the feed preparing raise poultry or domestic animal.
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| CN102919513A (en) * | 2012-11-07 | 2013-02-13 | 南开大学 | Method for producing feed by using fungi to ferment Chinese medicine residue |
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