CN104171279B - A kind of pig starter feed resisting stress additive and its preparation method and application - Google Patents
A kind of pig starter feed resisting stress additive and its preparation method and application Download PDFInfo
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- CN104171279B CN104171279B CN201410387238.4A CN201410387238A CN104171279B CN 104171279 B CN104171279 B CN 104171279B CN 201410387238 A CN201410387238 A CN 201410387238A CN 104171279 B CN104171279 B CN 104171279B
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Abstract
The invention discloses a kind of pig starter feed resisting stress additive and its preparation method and application, belongs to agriculture herding application field.Resisting stress additive provided by the present invention composition is tunning 5% 10%, tryptophan 10 25%, glutamine 15 20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1 3%, Fructus Ligustri Lucidi polysaccharide 0.1 0.2%, astragalus polyose 0.1 0.3%, pachymaran 0.1 0.2%, vitamin E 0.1 0.4%, remaining is zeolite powder.Wherein tunning is after agricultural wastes straw is hydrolyzed into glucose, by the use of liquid glucose as matrix culture Lactobacillus plantarum and saccharomyces cerevisiae, bacterium powder that zymotic fluid obtains after spray drying.Pig starter feed resisting stress additive provided by the present invention can increase lie prone sleeping behavior of piglet and reduce standing behavior, reduce piglet because mixed group stress caused by salivary cortisol content and blood epinephrine contents raise, and improve immunoglobulin content in piglet blood, and then alleviate piglet mix group after stress behavior, lifted pigling immunity.
Description
Technical field
The present invention relates to a kind of pig starter feed resisting stress additive and its preparation method and application, belongs to agriculture herding application
Field.
Background technology
It can cause mental depression, neuro-endocrinology hormone imbalance and provocative act in response to swashing after weaned piglet and mixed group
Occur, cause feed intake, gastrointestinal health, growth performance, immunity function etc. to decline.The current side that solves piglet production and stress commonly use
Method is that antibiotic, calm medicine etc. are added in feed.Although the use of this kind of medicine achieves preferable effect, make for a long time
The problems such as with easily resistance and antibiotic residue is caused.With the enhancing of health of people and environmental consciousness, green,
Pollution-free, noresidue anti-stress feed addictive has turned into the inexorable trend of sustainable development of animal husbandry.At present, with people
Concern to animal welfare and animal health, antibioticses calm safety of the medicine as feed addictive by the height of people
Degree is paid attention to.Under this background, Animal nutrition scholar points out, pays close attention to the anti-stress nutrition of piglet, lifts piglet disease-resistant power, reduces
Piglet disease problems caused by weaning and mixing group and stress wait are urgently to be resolved hurrily.How ablactation stress is overcome to caused by piglet production
Harm, it is emphasis and difficult point that pig industry is concerned about all the time.Therefore, selection it is a kind of using safe and effective feed addictive come
The generation for improving the factors such as piglet fighting behavior caused by ablactation stress, growth performance decline, disease is extremely urgent.
Stalk be common agricultural wastes, the more maize plantings in the Northeast, therefore stalk mainly based on maize straw, its
Main component is cellulose, lignin and hemicellulose.At present, peasant handles stalk by the way of burning more, not only pollutes
Environment, and wherein biomass can not be also utilized.On the research of stalk new application, focus mostly in biogas fermentation, make
For directions such as the high level compositions such as straw feed or fermented feed, extraction lignin.Stalk prepares fermented feed for raw material to be needed to sieve
Select fermentation inhibitor and corrupt inhibitor, fermentation inhibitor such as hydrochloric acid, propionic acid and salt, corrupt inhibitor such as formic acid, benzoic acid
And formaldehyde, it reduce further the palatability of feed.Crude fiber content is still very high in fermented feed at present, prior art for
Crude fibre treatment effect is not notable.The value of maize straw how is improved, is that current feedstuff industry is in the urgent need to address
Technical problem.
The content of the invention
The invention provides a kind of pig starter feed resisting stress additive, this stress the composition of additive be tunning 5%-
10%, tryptophan 10-25%, glutamine 15-20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1-3%, Fructus Ligustri Lucidi polysaccharide 0.1-0.2%, the Radix Astragali are more
Sugared 0.1-0.3%, pachymaran 0.1-0.2%, vitamin E 0.1-0.4%, remaining is zeolite powder；
The tunning is after agricultural wastes straw is hydrolyzed into glucose, and matrix culture plant is used as by the use of liquid glucose
Lactobacillus and saccharomyces cerevisiae, the bacterium powder that zymotic fluid obtains after spray drying.
The agricultural wastes straw method for hydrolysis is as follows：Stalk crush after according to solid-liquid ratio be 1:0.5, which adds water, is carried out
Steam explosion treatment, steam blasting Pretreatment pressures are 120s in 1.5-2.0MPa, reservation pressure time；Stalk is used after explosion
PH5.0 buffer solution regulation solid-liquid ratio is 1:5-1:10, add pectase 50U/g, cellulase 20U/g and half by dry matter weight
Cellulase 20U/g, digested 1-3 days in 40 DEG C, supernatant is obtained using plate compression or centrifuge.
The liquid glucose adds peptone, yeast extract, diammonium hydrogen citrate, calcium salt, sylvite, magnesium salts, sub- selenium after detoxification
It is used to cultivate Lactobacillus plantarum and saccharomyces cerevisiae after sour sodium and sodium glutamate.
Another object of the present invention is to provide a kind of preparation method of pig starter feed resisting stress additive, this method be by
After agricultural wastes straw is hydrolyzed to glucose, matrix culture Lactobacillus plantarum and saccharomyces cerevisiae, zymotic fluid are used as by the use of liquid glucose
Tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran, dimension are added after spray drying
Resisting stress additive is made in raw plain E, zeolite powder.
Methods described be stalk crush after according to solid-liquid ratio be 1:0.5, which adds water, carries out Steam explosion treatment, steam blasting
Pretreatment pressures are 120s in 1.5-2.0MPa, reservation pressure time；Stalk adjusts solid-liquid ratio with pH5.0 buffer solution after explosion
For 1:5-1:10, pectase 50U/g, cellulase 30U/g and hemicellulase 20U/g are added by dry matter weight, in 40 DEG C of enzymes
Solution, supernatant is obtained using plate compression or centrifuge；Supernatant adds peptone, yeast extract, hydrogen citrate after detoxification
It is used to cultivate Lactobacillus plantarum and saccharomyces cerevisiae after two ammoniums, calcium salt, sylvite, magnesium salts, sodium selenite and sodium glutamate.
Methods described comprises the following steps that：
1) it is 1 according to solid-liquid ratio after stalk crushing:0.5, which adds water, carries out Steam explosion treatment, steam blasting pretreatment pressure
Power is 120s in 1.5-2.0MPa, reservation pressure time；
2) it is 1 that stalk adjusts solid-liquid ratio with pH5.0 buffer solution after explosion:5-1:10, add pectase by dry matter weight
50U/g, cellulase 30U/g and hemicellulase 20U/g, digest 1-3 days in 40 DEG C, obtained using plate compression or centrifuge
Supernatant；
3) liquid glucose is diluted to concentration of reduced sugar below 20% after detoxification, adds peptone, yeast extract, hydrogen citrate
It is used to cultivate Lactobacillus plantarum and saccharomyces cerevisiae after two ammoniums, calcium salt, sylvite, magnesium salts, sodium selenite and sodium glutamate；Wherein, it is living
Bacterium number is 108CFU/mL Lactobacillus plantarums inoculum concentration is 5%, 107CFU/mL saccharomyces cerevisiaes inoculum concentration is 1%；Cultivation temperature is 32
DEG C culture 18 hours；
4) zymotic fluid is spray-dried to obtain powdered xeraphium after adding maltodextrin and milk powder, is heated to plant
It is more with tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, Poria cocos after lactobacillus and saccharomyces cerevisiae inactivation
Resisting stress additive is made in sugar, vitamin E, zeolite powder.
20mg/L sodium selenites, 75mg/L sodium glutamates, 10g/L peptones, 5g/L are added in the step 3) of methods described
Yeast extract, 0.2g/L diammonium hydrogen citrates, 0.2g/L calcium salts, 0.1g/L sylvite and 0.1g/L magnesium salts.
The resisting stress additive be used for alleviate piglet mix group after stress behavior.
The method that stalk of the present invention is hydrolyzed to glucose, it is first by stalk after pretreatment, is obtained by enzymolysis
Enzymolysis liquid.Applicant was once attempted to carry out acidolysis processing in shaking flask, and in acid hemolysis process, grape is obtained using two-step method sulphuric acid hydrolysis
Sugared yield is higher, but it can produce higher concentration sulfate radical.
The specific method that stalk is hydrolyzed to glucose is：Be stalk crush after according to solid-liquid ratio be 1:0.5, which adds water, is carried out
Steam explosion treatment, steam blasting Pretreatment pressures are 120s in 1.5-2.0MPa, reservation pressure time；Stalk is used after explosion
PH5.0 buffer solution regulation solid-liquid ratio is 1:5-1:10, add pectase 50U/g, cellulase 30U/g and half by dry matter weight
Cellulase 20U/g, digested 1-3 days in 40 DEG C, after enzyme deactivation, supernatant is obtained using plate compression or centrifuge；Supernatant passes through
Cross after detoxification add after peptone, yeast extract, diammonium hydrogen citrate, calcium salt, sylvite and magnesium salts be used to cultivating Lactobacillus plantarum and
Saccharomyces cerevisiae.
The liquid glucose is the other compositions that culture medium is added after detoxification, and the detoxification is the resin D301 that will first activate
According to solid-liquid ratio 5:Sealed in 1 enzymolysis liquid added to after neutralizing, 12h is shaken at 24 DEG C, is filtered after 12h, taken supernatant to survey sugar and treat
With.
Zymotic fluid is spray-dried to obtain powdered xeraphium after adding 5% maltodextrin, is heated to Lactobacillus plantarum
With saccharomyces cerevisiae inactivate after, take powder based on 5%-10% weight with tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, the fruit of glossy privet
Resisting stress additive is made in polysaccharide, astragalus polyose, pachymaran, vitamin E, zeolite powder.
Wherein, tunning 5%-10%, tryptophan 10-25%, glutamine 15-20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1-3%,
Fructus Ligustri Lucidi polysaccharide 0.1-0.2%, astragalus polyose 0.1-0.3%, pachymaran 0.1-0.2%, vitamin E 0.1-0.4%, remaining
For zeolite powder.
Present invention also offers a kind of application of pig starter feed resisting stress additive, is to add one in piglet basal diet
The above-mentioned additive of certainty ratio, its adding proportion are preferably 0.5-1.5%, can be according to the specific daily behavior and fighting behavior of pig
Determine additive addition.
The present invention has the advantages that：
1) while realizing straw waste recycling, discarded object is improved by way of microbial fermentation prepares additive
The value of stalk, Reducing sugar reaches more than 20% after hydrolysis；
2) cellulose in stalk is converted into by glucose using the method for explosion and enzymolysis, avoids sulfuric acid in acid hemolysis process
The generation of root, and the yield of glucose is improved, acid hydrolysis solution can use after detoxification directly as culture medium, avoid fibre
The pressure of sewage disposal after dimension element hydrolysis；
3) liquid glucose is digested as culture medium using stalk, realizes the co-cultivation of Lactobacillus plantarum and saccharomycete；
4) the resisting stress additive for preparing of the present invention is added in feed, piglet is added and lies prone and sleeping behavior and reduce standing row
For, reduce piglet because mixed group stress caused by salivary cortisol content and the rise of blood epinephrine contents, and improve son
Immunoglobulin content in pig blood, so alleviate piglet mix group after stress behavior, lifted pigling immunity.
Brief description of the drawings
Influence of Fig. 1 cellulases addition to Reducing sugar.
The photo of bacterium powder after Fig. 2 spray drying.
Embodiment
With reference to specific embodiment, the present invention will be further described, but the present invention should not be limited by the examples.
The stalk of embodiment 1 is hydrolyzed to the research of glucose
Present embodiments provide a kind of stalk be hydrolyzed to glucose sugar method, be after stalk is crushed according to solid-liquid ratio be 1:
0.5, which adds water, carries out Steam explosion treatment, and steam blasting Pretreatment pressures are 120s in 1.5-2.0MPa, reservation pressure time；
Stalk is 1 with pH5.0 buffer solution regulation solid-liquid ratio after explosion:5-1:10, add pectase, hemicellulase by dry matter weight
And cellulase, digested 24 hours in 50 DEG C, after enzyme deactivation, supernatant is obtained using plate compression or centrifuge；Steam blasting is pre-
Processing pressure is in 1.5-2.0MPa, it is impossible to more than 2.0MPa, otherwise can produce more furfural class accessory substance, steam blasting reason exists
Pre-processed in stalk.PH to 5.0-6.0 or so can be adjusted after explosion with a small amount of phosphate buffer.
Add enzyme according to following 4 kinds of modes respectively to handle sample, treatment temperature is 50 DEG C, is digested 24 hours.Add
When adding cellulase 10-50U/g, Reducing sugar is as shown in Figure 1.It is relatively low in view of Reducing sugar, further, in fiber
Pectase and hemicellulase are added on the basis of plain enzyme, when pectase addition is 50U/g, hemicellulase addition is 20U/
G, Reducing sugar are up to 41.8%.
The influence that the stalk hydrolyzate concentration of reduced sugar of embodiment 2 grows to fermented bacterium
Enzymolysis liquid is diluted to obtain the liquid glucose of different content of reducing sugar, blank is that Lactobacillus plantarum or saccharomycete are individually cultivated
When, using spectrophotometric determination OD values, as a result as shown in table 1.As seen from Table 1 when enzymolysis liquid content of reducing sugar is higher than
20%, the growth to Lactobacillus plantarum can produce significant impact.Possible cause is, accessory substance caused by stalk hydrolytic process
Inhibit the growth of Lactobacillus plantarum.After enzymolysis by way of dilution, concentration of reduced sugar is maintained less than 20%.
The influence that the concentration of reduced sugar of table 1 grows to fermented bacterium
The Lactobacillus plantarum of embodiment 3 is co-cultured with saccharomycete and studied
Lactobacillus plantarum is Lactobacillus plantarum ATCC purchased from Unite States Standard strain library ATCC numberings
14917, the bacterial strain freezes (subzero 70-80 degrees Celsius) in 15% glycerine, freezes after pipe takes out on MRS inclined-planes solid medium
Activate picking individual colonies, after the liquid glucose being seeded to after two generations after enzymolysis by detoxification treatment is activated in MRS fluid nutrient mediums.
Prepare saccharomyces cerevisiae seed culture medium：Saccharomyces cerevisiae strain is transferred on saccharomyces cerevisiae seed culture medium and lived
Change, 30 DEG C are cultivated 24 hours；Picking activation saccharomyces cerevisiae strain inoculation saccharomyces cerevisiae fluid nutrient medium 5mL, 30 DEG C of cultures 24 are small
When, spread cultivation successively according to 10% inoculum concentration.The seed culture medium composition：Yeast extract 5g, peptone 10g, sodium chloride
5g, agar 15g, water 1000mL, pH value are 7 preparation saccharomyces cerevisiae seed culture mediums；Saccharomyces cerevisiae strain liquid：Yeast extract
5g, peptone 10g, sodium chloride 5g, agar 15g, water 1000mL, pH value are 7 preparation saccharomyces cerevisiae strain liquid, and 121 DEG C sterilize
30 minutes.Saccharomyces cerevisiae is ATCC74366 purchased from ATCC numberings.
Before fermentation, add sodium selenite in the liquid glucose after detoxification treatment and sodium glutamate, wherein sodium selenite are dense
Spend as shown in the table.Growth of the high concentration sodium selenite to Lactobacillus plantarum and saccharomyces cerevisiae generates obvious inhibitory action,
But when concentration of sodium selenite is 10-50mg/L, obvious inhibitory action is not produced, wherein when concentration of sodium selenite is
During 20mg/L, sodium selenite conversion ratio highest.
Influence of the concentration of sodium selenite of table 2 to Lactobacillus plantarum and saccharomyces cerevisiae biomass
Equally, experiment finds that Lactobacillus plantarum and saccharomyces cerevisiae can not be degradable by sodium glutamate, and sodium glutamate
γ-aminobutyric acid can not be fully converted to, when addition is higher than 100mg/L sodium glutamates, sodium glutamate residual quantity is higher, shadow
Its mouthfeel as feed addictive is rung.Therefore, sodium glutamate addition should be less than 100mg/L.
The change of the sodium glutamate of table 3 existence form in fermentation system
| Concentration of sodium glutamate | Sodium glutamate remains | γ-aminobutyric acid |
| 20mg/L | 2.17mg/L | 4.15mg/L |
| 50mg/L | 3.03mg/L | 5.03mg/L |
| 70mg/L | 5.15mg/L | 10.8mg/L |
| 100mg/L | 9.52mg/L | 12.5mg/L |
| 150mg/L | 35mg/L | 11.17mg/L |
Specific spray drying process is as follows：Spray drying device is passed through clear water using preceding elder generation and washed in a device, by equipment
In residue wash out, after be passed through more than 200 DEG C of high temperature air 15-30min, after equipment cooling, by the good cow's milk of homogeneous
Charging aperture is put into maltodextrin, starts to spray.Outlet temperature is set during spraying as 80 DEG C, feeding temperature is selected 120 DEG C and 100
DEG C two temperature.Bacterium powder (bacterium powder after spray drying is as shown in Figure 2) is collected, viable count is determined with viable bacteria counting method.
Embodiment 4 mixes with other raw materials
Tryptophan, amide glutaminate, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran, vitamin E is broken
Into the particle of 60 mesh；
Carrier zeolite powder is ground into the particle of 200 mesh；
Above-mentioned raw materials composition is weighed in the ratio in formula, is then put into the stainless steel mixing of mixture homogeneity≤5%
In machine stir 5-8 minutes, by quantitative package finished product；
The said goods are mixed into bacterium powder prepared by embodiment 3 according to a certain percentage, specific adding proportion is as follows：Fermentation production
Thing 5%-10%, tryptophan 10-25%, glutamine 15-20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1-3%, Fructus Ligustri Lucidi polysaccharide 0.1-0.2%,
Astragalus polyose 0.1-0.3%, pachymaran 0.1-0.2%, vitamin E 0.1-0.4%, remaining is zeolite powder.It will be prepared
Above-mentioned additive be added in 1% ratio in piglet diet, fed and managed by the conventinal breeding program of pig farm piglet.
Embodiment 5 mixes with other raw materials
Scheme 1
Tunning 5%, tryptophan 10%, glutamine 15%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1%, Fructus Ligustri Lucidi polysaccharide 0.1% are yellow
Astragalus polysaccharides 0.1%, pachymaran 0.1%, vitamin E 0.1%, remaining is zeolite powder.
Scheme 2
Tunning 7.5%, tryptophan 15%, glutamine 18%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 2%, Fructus Ligustri Lucidi polysaccharide 0.1%,
Astragalus polyose 0.2%, pachymaran 0.1%, vitamin E 0.2%, remaining is zeolite powder.
Scheme 3
Tunning 10%, tryptophan 25%, glutamine 20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 3%, Fructus Ligustri Lucidi polysaccharide 0.2% are yellow
Astragalus polysaccharides 0.3%, pachymaran 0.2%, vitamin E 0.4%, remaining is zeolite powder.
The application experiment of embodiment 6
The above-mentioned additive being prepared is added in piglet diet in 1% ratio, by the conventinal breeding of pig farm piglet
Program is fed and managed.Additive composition is as follows：Tunning 7.5%, tryptophan 15%, glutamine 18%, thorn five
Sugar 2% is added, Fructus Ligustri Lucidi polysaccharide 0.1%, astragalus polyose 0.2%, pachymaran 0.1%, vitamin E 0.2%, remaining is zeolite
Powder.
Experiment packet and diet
160 healthy 28 ages in days wean castrated piglets are randomly divided into 2 groups, i.e. control group, test group by body weight, every group
It is each to repeat have 8 pigs if 10 repetitions.Control group fed Basic drawing, basal diet formula is with reference to bacon hogs raising mark
Accurate (2004) and U.S. NRC (2012) pig nutritional need is prepared.Test group is added 1% pig starter feed in basal diet and resisted
Stress additive.
Feeding management
Experiment is carried out on long-range kind of pig farm, and pig house is concrete floor.There are a hopper and automatic drinking bowl in often enclosing.Pig
Raising and immune long-range animal husbandry pig farm Routine Management of pressing are carried out.Test piglet and choose 28 days weanling pigs, original body mass is 8 kg
Left and right.Primitive pit go to nursery house feed 30 days, experiment the 10th day to the 15th day 14:00-16:00 observation piglet stand, lie prone it is sleeping,
Dog sits back and waits daily behavior, and the piglet that feeding in 30 days terminates between rear same treatment group mixes group and (each repeats 4 piglets of any selection to do
Mark mixes mutually group), feeding daily ration is constant, using CCD infrared digital camera (models：SG-IR612MP, Sage, Shenzhen) even
Continuous observation 8h, the number fought in detail in the every column piglet 8h of record and time simultaneously collect saliva measure salivary cortisol.Examination
One pig of each repetition extraction butchers collection blood measuring blood adrenaline after testing end.
Result of the test：
The influence of each treatment group piglet daily behavior is as shown in table 4.As shown in Table 4, with feed basal diet control group
Compare, the dog seat behavior difference of test group piglet is not notable, but sleeping behavior of lying prone dramatically increases, and standing behavior significantly reduces, and shows
Adding the piglet daily behavior of pig starter feed resisting stress additive of the present invention is improved.
The comparison of each treatment group piglet daily behavior of table 4
| Daily behavior | Control group | Test group |
| Lie prone sleeping | 65.08b | 73.56a |
| Stand | 20.23b | 14.25a |
| Dog sits | 3.45 | 3.51 |
Compare in factor identical with a line, the female different persons of marking-up represent significant difference (P ＜ 0.05).
The influence that fighting behavior after group is mixed to piglet is as shown in table 5.As shown in Table 5, with feed basal diet control group
Compare, test group piglet fight the time and number of fighting all significantly reduces, illustrate to add pig starter feed resisting stress additive energy
Significantly reduce piglet and mix the behavior of fighting after group.
Each treatment group piglet of table 5 mix group after in 8 hours fighting behavior comparison
| Fight behavior | Control group | Test group |
| Fight the time (second) | 111.84±7.13a | 35.89±3.09b |
| Fight number | 15.03±0.94a | 8.21±0.23b |
Compare in factor identical with a line, the female different persons of marking-up represent significant difference (P ＜ 0.05).
Each treatment group is as shown in table 6 on piglet salivary cortisol content, the adrenergic influence of blood.As shown in Table 6, with
The control group of feeding basal diet is compared, and the salivary cortisol content and blood epinephrine contents of test group piglet significantly drop
It is low, illustrate to test the pig starter feed resisting stress additive that the present invention is added in piglet diet can significantly reduce with stress be related it is sharp
Cellulose content.
6 each treatment group of table is to piglet salivary cortisol content, the adrenergic comparison of blood
| Index | Control group | Test group |
| Cortisol (ng/ml) | 3.13±0.05a | 1.27±0.01b |
| Adrenaline (pg/mL) | 112.70±1.23a | 56.56±1.23b |
In identical factor of going together relatively, the female different persons of marking-up represent significant difference (P ＜ 0.05).
Influence of each treatment group to piglet immunological globulin content is as shown in table 7.As shown in Table 7, with feeding basal diet
Control group compare, immunoglobulin M (IgM) content is without significant difference in test group piglet blood, but test group piglet blood
Immunoglobulin A (IgA) and immunoglobulin G (IgG) content are significantly higher than control group, illustrate to add this hair in piglet diet
Bright pig starter feed resisting stress additive can improve piglet immunological globulin content, alleviate piglet because wean and mix group stress caused by
Pigling immunity declines.
Comparison of the 7 each treatment group of table to piglet immunological globulin content
| Index | Control group | Test group |
| IgA | 0.19±0.05b | 0.27±0.01a |
| IgG | 2.92±0.12b | 3.37±0.10a |
| IgM | 0.29±0.02 | 0.30±0.05 |
In identical factor of going together relatively, the female different persons of marking-up represent significant difference (P ＜ 0.05).
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this
The people of technology, without departing from the spirit and scope of the present invention, various changes and modification, therefore the protection of the present invention can be done
What scope should be defined by claims is defined.
Claims (5)
1. a kind of pig starter feed resisting stress additive, it is characterised in that tunning 5-10%, tryptophan 10-25%, glutamy
Amine 15-20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1-3%, Fructus Ligustri Lucidi polysaccharide 0.1-0.2%, astragalus polyose 0.1-0.3%, pachymaran 0.1-
0.2%, vitamin E 0.1-0.4%, remaining is zeolite powder；The tunning is that agricultural wastes straw is hydrolyzed into grape
After sugar, by the use of liquid glucose as matrix culture Lactobacillus plantarum and saccharomyces cerevisiae, bacterium powder that zymotic fluid obtains after spray drying；
Wherein, the liquid glucose adds 20mg/L sodium selenites, 75mg/L sodium glutamates, 10g/L peptones, 5g/L after detoxification
It is used to cultivate plant breast bar after yeast extract, 0.2g/L diammonium hydrogen citrates, 0.2g/L calcium salts, 0.1g/L sylvite and 0.1g/L magnesium salts
Bacterium and saccharomyces cerevisiae；The Lactobacillus plantarum is Lactobacillus plantarum ATCC 14917.
2. resisting stress additive described in claim 1, it is characterised in that the agricultural wastes straw method for hydrolysis is as follows：Straw
According to solid-liquid ratio 1 after stalk crushing:0.5, which adds water, carries out Steam explosion treatment, and steam blasting Pretreatment pressures are 1.5-2.0MPa,
Reservation pressure time is 120s；Stalk is 1 with pH 5.0 buffer solution regulation solid-liquid ratio after explosion:5-1:10, by dry matter weight
Add pectase 50U/g, cellulase 20U/g and hemicellulase 20U/g, digested 1-3 days in 40 DEG C, using plate compression or
Centrifuge obtains supernatant.
3. the preparation method of resisting stress additive described in a kind of claim 1, it is characterised in that be by agricultural wastes straw water
Solve as after glucose, by the use of liquid glucose as matrix culture Lactobacillus plantarum and saccharomyces cerevisiae, zymotic fluid adds after spray drying
Enter tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran, vitamin E, zeolite powder to be made
Resisting stress additive.
4. claim 3 methods described, it is characterised in that be according to solid-liquid ratio 1 after stalk crushes:0.5, which adds water, carries out steam
Explosion treatment, for steam blasting Pretreatment pressures in 1.5-2.0MPa, reservation pressure time is 120s；Stalk pH 5.0 after explosion
Buffer solution regulation solid-liquid ratio be 1:5-1:10, add pectase 50U/g, cellulase 30U/g and hemicellulose by dry matter weight
Plain enzyme 20U/g, in 40 DEG C of enzymolysis, supernatant is obtained using plate compression or centrifuge；Supernatant adds 20mg/ after detoxification
L sodium selenites, 75mg/L sodium glutamates, 10g/L peptones, 5g/L yeast extracts, 0.2g/L diammonium hydrogen citrates, 0.2g/L calcium
It is used to cultivate Lactobacillus plantarum and saccharomyces cerevisiae after salt, 0.1g/L sylvite and 0.1g/L magnesium salts.
5. claim 3 methods described, it is characterised in that comprise the following steps that：
1) it is 1 according to solid-liquid ratio after stalk crushing:0.5, which adds water, carries out Steam explosion treatment, and steam blasting Pretreatment pressures exist
1.5-2.0MPa reservation pressure time is 120s；
2) it is 1 that stalk adjusts solid-liquid ratio with pH 5.0 buffer solution after explosion:5-1:10, add pectase 50U/ by dry matter weight
G, cellulase 30U/g and hemicellulase 20U/g, digested 1-3 days in 40 DEG C, supernatant is obtained using plate compression or centrifuge
Liquid；
3) liquid glucose is diluted to concentration of reduced sugar below 20% after detoxification, adds peptone, yeast extract, hydrogen citrate two
It is used to cultivate Lactobacillus plantarum and saccharomyces cerevisiae after ammonium, calcium salt, sylvite, magnesium salts, sodium selenite and sodium glutamate；Wherein, viable bacteria
Number is 108CFU/mL Lactobacillus plantarums inoculum concentration is 5%, 107CFU/mL saccharomyces cerevisiaes inoculum concentration is 1%；Cultivation temperature is 32 DEG C
Culture 18 hours；
4) zymotic fluid is spray-dried to obtain powdered xeraphium after adding maltodextrin and milk powder, is heated to plant breast bar
Bacterium and saccharomyces cerevisiae inactivation after with tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran,
Resisting stress additive is made in vitamin E, zeolite powder.
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| CN104905075A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing pig complete feed by solid state enzyme solution and fermentation |
| CN104905074A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing laying duck complete feed by solid state enzyme solution and fermentation |
| CN104905013A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing protein feed raw materials by carrying out enzymolysis and fermentation on white spirit vinasse |
| CN104920805A (en) * | 2015-06-30 | 2015-09-23 | 河南双成生物科技有限公司 | Method for producing antimicrobial-free feed through enzymosis and fermentation of straw |
| CN104905018A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing protein feed material by enzymolysis and fermentation of guar meal |
| CN104905073A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing meat duck complete feed by solid state enzyme solution and fermentation |
| CN104904993A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing cattle and sheep feed by solid state enzyme solution and fermentation |
| CN104904991A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing protein feed raw materials by carrying out enzymolysis and fermentation on soybean meal |
| CN105076721B (en) * | 2015-08-25 | 2018-03-20 | 江西优能生物科技有限公司 | A kind of pre-mixing agent, preparation method and purposes for reducing weanling pig oxidative stress |
| CN105394413A (en) * | 2015-11-12 | 2016-03-16 | 六安瑞泰养殖科技有限公司 | Calcium-supplementing piglet feed capable of improving immunity and preparation method of calcium-supplementing piglet feed |
| CN107751612A (en) * | 2017-11-23 | 2018-03-06 | 贵州大兴农业科技发展有限公司 | A kind of feed for feeding 13 months pigs and preparation method thereof |
| CN109275781A (en) * | 2018-11-19 | 2019-01-29 | 天水市畜牧技术推广站 | It is a kind of using paper mulberry as poultry anti-stress feed of raw material and preparation method thereof |
| CN113198007A (en) * | 2021-04-15 | 2021-08-03 | 河南省帮邦生物工程有限公司 | Compound preparation for clearing pig endotoxin and preparation method thereof |
| CN114732084A (en) * | 2022-03-04 | 2022-07-12 | 扬州日兴生物科技股份有限公司 | Method for preparing anti-stress animal feed by biological processing of shrimp processing by-products |
| CN114532444A (en) * | 2022-03-08 | 2022-05-27 | 四川农业大学 | Fibrous thallus protein feed and preparation method thereof |
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| CN103429749B (en) * | 2010-12-10 | 2016-12-14 | 诺维信公司 | For the method producing tunning from lignocellulose-containing materials |
| CN102488089A (en) * | 2011-11-18 | 2012-06-13 | 吉林省农业科学院 | Preparation method of microecological culture feed additive |
| CN103315184B (en) * | 2013-07-11 | 2014-03-26 | 曾勇 | Feed additive for alleviating unfavorable influences of transport stress to growth of piglets |
| CN103349186B (en) * | 2013-08-01 | 2014-08-20 | 正大康地（澄海）有限公司 | Weaned piglet feed additive and preparation method thereof |
| CN103549184A (en) * | 2013-10-31 | 2014-02-05 | 北京福乐维生物科技股份有限公司 | Nano nutritious supplementary with function of fattening pigs and preparation method thereof |
| CN103598483B (en) * | 2013-11-22 | 2016-04-13 | 江瀚生物科技（上海）有限公司 | A kind of reduce piglet stress additive for microbe feedstuff and preparation method thereof |
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Inventor after: Chen Junhai Inventor after: Liu Huawei Inventor after: Shu Danping Inventor after: Zhang Xiaojie Inventor after: Zeng Minying Inventor after: Wei Kejian Inventor after: Xiao Huali Inventor before: Chen Wenbin Inventor before: Zhang Xiaojie Inventor before: Liu Huawei Inventor before: Li Yuxia |
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Effective date of registration: 20160713 Address after: 518106 Guangdong province Shenzhen Guangming New District Office in Gongming will be on the stone stone community on the South Industrial Zone Applicant after: SHENZHEN JINXINNONG FEED CO., LTD. Applicant after: Haerbin Yuanda Animal Husbandry Co.,Ltd Applicant after: Shanghai Chengnong Feed Co.,Ltd. Applicant after: Guangdong golden Feed Co., Ltd. Applicant after: Fujian Jin Xinnong Feed Co., Ltd. Applicant after: Changsha Cheng Nong feedstuff Co., Ltd. Applicant after: Sichuan Golden Dragon Feed Co., Ltd. Address before: 150060 Heilongjiang city of Harbin province Pingfang District haping Road District Bohai Road No. 8 Applicant before: Haerbin Yuanda Animal Husbandry Co.,Ltd |
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Address after: 518106 Guangdong province Shenzhen Guangming New District Office in Gongming will be on the stone stone community on the South Industrial Zone Applicant after: Shenzhen Jin Xinnong Polytron Technologies Inc Applicant after: Haerbin Yuanda Animal Husbandry Co.,Ltd Applicant after: Shanghai Chengnong Feed Co.,Ltd. Applicant after: Guangdong golden Feed Co., Ltd. Applicant after: Fujian Jin Xinnong Feed Co., Ltd. Applicant after: Changsha Cheng Nong feedstuff Co., Ltd. Applicant after: Sichuan Golden Dragon Feed Co., Ltd. Address before: 518106 Guangdong province Shenzhen Guangming New District Office in Gongming will be on the stone stone community on the South Industrial Zone Applicant before: SHENZHEN JINXINNONG FEED CO., LTD. Applicant before: Haerbin Yuanda Animal Husbandry Co.,Ltd Applicant before: Shanghai Chengnong Feed Co.,Ltd. Applicant before: Guangdong golden Feed Co., Ltd. Applicant before: Fujian Jin Xinnong Feed Co., Ltd. Applicant before: Changsha Cheng Nong feedstuff Co., Ltd. Applicant before: Sichuan Golden Dragon Feed Co., Ltd. |
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