CN104171279A - Piglet fodder anti-stress additive as well as preparation method and application thereof - Google Patents
Piglet fodder anti-stress additive as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a piglet fodder anti-stress additive as well as a preparation method and an application thereof and belongs to the field of agriculture stockbreeding application. The anti-stress additive provided by the invention comprises 5%-10% of fermentation product, 10-25% of tryptophan, 15-20% of glutamine, 1-3% of acanthopanax senticosus polysaccharide, 0.1-0.2% of fructus ligustri lucidi polysaccharides, 0.1-0.3% of astragalus polysaccharide, 0.1-0.2% of pachymaran, 0.1-0.4% of vitamin E and the balance of zeolite powder. The fermentation product is microbial inoculant powder prepared by the steps of hydrolyzing agricultural waste straw into glucose, culturing lactobacillus plantarum and brewer's yeast by taking the liquid glucose as a matrix, and carrying out spray drying on fermentation liquor. By utilizing the piglet fodder anti-stress additive provided by the invention, the lying behavior of piglets can be increased, the standing behavior can be reduced, rise of saliva cortisol content and blood epinephrine content, caused by group stress of the piglets is reduced, immune globulin content in piglet blood is improved, and further the stress behavior of the piglets in a group is relieved and the immunity of the piglets is improved.
Description
Technical field
The present invention relates to that a kind of pig starter feed is anti-stress additive and its preparation method and application, belongs to agriculture herding application.
Background technology
After weaned piglet and mixed group, can, because causing the generation of mental depression, neuro-endocrinology hormone imbalance and provocative act, cause the declines such as feed intake, gastrointestinal health, growth performance, immunity function.It stress conventional method be in feed, to add antibiotic, calm medicine etc. that current solution piglet produces.Although the use of this class medicine has obtained good effect, long-term use easily causes the problem such as resistance and antibiotic residue.Along with the enhancing of health of people and environmental consciousness, environmental protection, anti-stress feed addictive pollution-free, noresidue have become the inexorable trend of sustainable development of animal husbandry.At present, along with the concern of people to animal welfare and animal health, the calm medicine of antibiotics has been subject to people's great attention as the safety of feed addictive.Under this background, Animal nutrition scholar points out, pays close attention to the anti-stress nutrition of piglet, promotes piglet disease-resistant power, reduces piglet because of wean and mixes group and stress wait the disease problem causing urgently to be resolved hurrily.How to overcome ablactation stress and piglet produced to the harm causing, be all the time raise pigs industry be concerned about Focal point and difficult point.Therefore, select the generation of the factors such as a kind of fighting behavior that adopts safe and effective feed addictive to improve piglet to cause because of ablactation stress, growth performance decline, disease extremely urgent.
Stalk is common agricultural wastes, the many maize plantings in the Northeast, and therefore stalk is mainly taking maize straw as main, and its main component is cellulose, lignin and hemicellulose.At present, peasant adopts the mode Treating straw of burning more, has not only polluted environment, and wherein living beings also cannot be utilized.About the research of the new purposes of stalk, focus mostly at biogas fermentation, as directions such as straw feed or fermented feed, the contour value compositions of extraction lignin.Stalk is that raw material is prepared fermented feed and need to be screened fermentation inhibitor and corrupt inhibitor, fermentation inhibitor example hydrochloric acid, propionic acid and salt, and corrupt inhibitor, as formic acid, benzoic acid and formaldehyde, has further reduced the palatability of feed.In fermented feed, crude fiber content is still very high at present, and prior art is not remarkable for crude fibre treatment effect.How improving the value of maize straw, is current feedstuff industry technical problem in the urgent need to address.
Summary of the invention
The invention provides that a kind of pig starter feed is anti-stress additive, this stress additive consist of tunning 5%-10%, tryptophan 10-25%, glutamine 15-20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1-3%, Fructus Ligustri Lucidi polysaccharide 0.1-0.2%, astragalus polyose 0.1-0.3%, pachymaran 0.1-0.2%, vitamin E 0.1-0.4%, all the other are zeolite powder;
Described tunning is that agricultural wastes straw is hydrolyzed to after glucose, utilizes liquid glucose to cultivate Lactobacillus plantarum and saccharomyces cerevisiae as matrix, the bacterium powder that zymotic fluid obtains after being dried through spraying.
Described agricultural wastes straw method for hydrolysis is as follows: after crushed stalk, be that 1:0.5 adds water to carry out steam blasting processing according to solid-liquid ratio, steam blasting pretreatment pressure is 120s at 1.5-2.0MPa, reservation pressure time; After explosion, stalk is 1:5-1:10 with the buffer solution adjusting solid-liquid ratio of pH5.0, adds pectase 50U/g, cellulase 20U/g and hemicellulase 20U/g by dry matter weight, in 40 DEG C of enzymolysis 1-3 days, adopts plate compression or centrifuge to obtain supernatant.
Described liquid glucose adds after peptone, yeast extract, diammonium hydrogen citrate, calcium salt, sylvite, magnesium salts, sodium selenite and sodium glutamate for cultivating Lactobacillus plantarum and saccharomyces cerevisiae after detoxification.
Another object of the present invention is to provide a kind of pig starter feed anti-preparation method that stress additive, the method is that agricultural wastes straw is hydrolyzed to after glucose, utilize liquid glucose to cultivate Lactobacillus plantarum and saccharomyces cerevisiae as matrix, zymotic fluid add after dry through spraying tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran, vitamin E, zeolite powder make anti-stress additive.
Described method is after crushed stalk to be that 1:0.5 adds water to carry out steam blasting processing according to solid-liquid ratio, and steam blasting pretreatment pressure is 120s at 1.5-2.0MPa, reservation pressure time; After explosion, stalk is 1:5-1:10 with the buffer solution adjusting solid-liquid ratio of pH5.0, adds pectase 50U/g, cellulase 30U/g and hemicellulase 20U/g by dry matter weight, in 40 DEG C of enzymolysis, adopts plate compression or centrifuge to obtain supernatant; Supernatant adds after peptone, yeast extract, diammonium hydrogen citrate, calcium salt, sylvite, magnesium salts, sodium selenite and sodium glutamate for cultivating Lactobacillus plantarum and saccharomyces cerevisiae after detoxification.
The concrete steps of described method are as follows:
1) after crushed stalk, be that 1:0.5 adds water to carry out steam blasting processing according to solid-liquid ratio, steam blasting pretreatment pressure is 120s at 1.5-2.0MPa, reservation pressure time;
2) after explosion, stalk is 1:5-1:10 with the buffer solution adjusting solid-liquid ratio of pH5.0, adds pectase 50U/g, cellulase 30U/g and hemicellulase 20U/g by dry matter weight, in 40 DEG C of enzymolysis 1-3 days, adopts plate compression or centrifuge to obtain supernatant;
3) liquid glucose is diluted to concentration of reduced sugar below 20% after detoxification, adds after peptone, yeast extract, diammonium hydrogen citrate, calcium salt, sylvite, magnesium salts, sodium selenite and sodium glutamate for cultivating Lactobacillus plantarum and saccharomyces cerevisiae; Wherein, viable count is 10
8cFU/mL Lactobacillus plantarum inoculum concentration is 5%, 10
7cFU/mL saccharomyces cerevisiae inoculum concentration is 1%; Cultivation temperature is 32 DEG C and cultivates 18 hours;
4) zymotic fluid adds the dry Powdered xeraphium that obtains of spraying after maltodextrin and milk powder, heating to after Lactobacillus plantarum and saccharomyces cerevisiae deactivation with tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran, vitamin E, zeolite powder make anti-stress additive.
The step 3 of described method) in add 20mg/L sodium selenite, 75mg/L sodium glutamate, 10g/L peptone, 5g/L yeast extract, 0.2g/L diammonium hydrogen citrate, 0.2g/L calcium salt, 0.1g/L sylvite and 0.1g/L magnesium salts.
Described anti-stress additive for alleviate after the mixed group of piglet stress behavior.
Stalk of the present invention is hydrolyzed to the method for glucose, be first by stalk after pretreatment, the enzymolysis liquid obtaining through enzymolysis.Applicant once attempted in shaking flask, carrying out acidolysis processing, in acidolysis process, adopt two-step method sulphuric acid hydrolysis to obtain glucose yield higher, but it can produce higher concentration sulfate radical.
The concrete grammar that stalk is hydrolyzed to glucose is: be after crushed stalk to be that 1:0.5 adds water to carry out steam blasting processing according to solid-liquid ratio, steam blasting pretreatment pressure is 120s at 1.5-2.0MPa, reservation pressure time; After explosion, stalk is 1:5-1:10 with the buffer solution adjusting solid-liquid ratio of pH5.0, adds pectase 50U/g, cellulase 30U/g and hemicellulase 20U/g by dry matter weight, in 40 DEG C of enzymolysis 1-3 days, goes out after enzyme, adopts plate compression or centrifuge to obtain supernatant; Supernatant adds after peptone, yeast extract, diammonium hydrogen citrate, calcium salt, sylvite and magnesium salts for cultivating Lactobacillus plantarum and saccharomyces cerevisiae after detoxification.
Described liquid glucose is other compositions that add culture medium after detoxification, and described detoxification is first the resin D301 having activated to be added in the enzymolysis liquid after neutralization and to be sealed according to solid-liquid ratio 5:1, at 24 DEG C of concussion 12h, after 12h, filters, and gets supernatant and surveys sugared stand-by.
Zymotic fluid adds the dry Powdered xeraphium that obtains of spraying after 5% maltodextrin, heating to after Lactobacillus plantarum and saccharomyces cerevisiae deactivation, get powder by the weighing scale of 5%-10% and tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran, vitamin E, zeolite powder make resist stress additive.
Wherein, tunning 5%-10%, tryptophan 10-25%, glutamine 15-20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1-3%, Fructus Ligustri Lucidi polysaccharide 0.1-0.2%, astragalus polyose 0.1-0.3%, pachymaran 0.1-0.2%, vitamin E 0.1-0.4%, all the other are zeolite powder.
The present invention also provides a kind of pig starter feed anti-application that stress additive, in piglet basal diet, to add a certain proportion of above-mentioned additive, its adding proportion is preferably 0.5-1.5%, can determine additive addition according to the concrete daily behavior of pig and fighting behavior.
The present invention has following beneficial effect:
1) when realizing stalk changing waste into resources, promoted the value of wastes straw by the ferment mode of preparing additive of microorganism, after hydrolysis, Reducing sugar reaches more than 20%;
2) adopting the method for explosion and enzymolysis is glucose by cellulose conversion in stalk, avoid the generation of sulfate radical in acidolysis process, and improved the yield of glucose, acid hydrolysis solution can directly use as culture medium after detoxification, has avoided the pressure of sewage disposal after cellulose hydrolysis;
3), taking stalk enzymolysis liquid glucose as culture medium, Lactobacillus plantarum and saccharomycetic cultivation have altogether been realized;
4) in feed, add prepared by the present invention anti-stress additive, increase lie prone sleeping behavior reduce the behavior of standing of piglet, saliva Determination of cortisol and the rising of blood epinephrine contents that piglet stress cause because of mixed group are reduced, and improve immunoglobulin content in piglet blood, and then alleviate after the mixed group of piglet stress behavior, promote pigling immunity.
Brief description of the drawings
The impact of Fig. 1 cellulase addition on Reducing sugar.
The spray photo of dried bacterium powder of Fig. 2.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention is not subject to the restriction of embodiment.
Embodiment 1 stalk is hydrolyzed to the research of glucose
A kind of method that the present embodiment provides stalk to be hydrolyzed to glucose sugar, is after crushed stalk, to be that 1:0.5 adds water to carry out steam blasting processing according to solid-liquid ratio, and steam blasting pretreatment pressure is 120s at 1.5-2.0MPa, reservation pressure time; After explosion, to regulate solid-liquid ratio with the buffer solution of pH5.0 be 1:5-1:10 to stalk, adds pectase, hemicellulase and cellulase by dry matter weight, in 50 DEG C of enzymolysis 24 hours, goes out after enzyme, adopts plate compression or centrifuge to obtain supernatant; Steam blasting pretreatment pressure, at 1.5-2.0MPa, can not exceed 2.0MPa, otherwise can produce more furfural class accessory substance, and steam blasting reason is stalk to carry out pretreatment.After explosion, can regulate pH to 5.0-6.0 left and right with a small amount of phosphate buffer.
Add enzyme according to following 4 kinds of modes respectively sample is processed, treatment temperature is 50 DEG C, enzymolysis 24 hours.While adding cellulase 10-50U/g, Reducing sugar as shown in Figure 1.Consider that Reducing sugar is lower, further, add pectase and hemicellulase on cellulase basis, when pectase addition is 50U/g, hemicellulase addition is 20U/g, and Reducing sugar is up to 41.8%.
The impact of embodiment 2 stalk hydrolyzate concentration of reduced sugar on fermented bacterium growth
Enzymolysis liquid dilution is obtained to the liquid glucose of different content of reducing sugar, blank while cultivating separately for Lactobacillus plantarum or saccharomycete, utilize spectrophotometric determination OD value, result is as shown in table 1.When enzymolysis liquid content of reducing sugar is higher than 20%, can produce significant impact to the growth of Lactobacillus plantarum as seen from Table 1.Possible cause is, the accessory substance that stalk hydrolytic process produces has suppressed the growth of Lactobacillus plantarum.After enzymolysis, by the mode of dilution, concentration of reduced sugar is maintained below 20%.
The impact of table 1 concentration of reduced sugar on fermented bacterium growth
Embodiment 3 Lactobacillus plantarums and saccharomycete be culture studies altogether
Lactobacillus plantarum is numbered Lactobacillus plantarum ATCC 14917 purchased from Unite States Standard strain library ATCC, this bacterial strain is at 15% glycerine frozen (subzero 70-80 degree Celsius), after freeze-drying pipe takes out, on MRS inclined-plane solid medium, single bacterium colony is selected in activation, after in MRS fluid nutrient medium, activate be seeded to enzymolysis after two generations after through the liquid glucose of detoxification treatment.
Preparation saccharomyces cerevisiae seed culture medium: saccharomyces cerevisiae bacterial classification is transferred on saccharomyces cerevisiae seed culture medium and is activated, cultivate 24 hours for 30 DEG C; Picking activation saccharomyces cerevisiae bacterial classification inoculation saccharomyces cerevisiae fluid nutrient medium 5mL, cultivates 24 hours, spreads cultivation successively according to 10% inoculum concentration for 30 DEG C.Described seed culture medium composition: yeast extract 5g, peptone 10g, sodium chloride 5g, agar 15g, water 1000mL, pH value is 7 preparation saccharomyces cerevisiae seed culture mediums; Saccharomyces cerevisiae bacterial classification liquid: yeast extract 5g, peptone 10g, sodium chloride 5g, agar 15g, water 1000mL, pH value is 7 preparation saccharomyces cerevisiae bacterial classification liquid, 121 DEG C of sterilizings 30 minutes.Saccharomyces cerevisiae is numbered ATCC74366 purchased from ATCC.
Before fermentation, add sodium selenite and sodium glutamate in the liquid glucose after detoxification treatment, wherein concentration of sodium selenite is as shown in the table.High concentration sodium selenite has produced obvious inhibitory action to the growth of Lactobacillus plantarum and saccharomyces cerevisiae, but in the time that concentration of sodium selenite is 10-50mg/L, do not produce obvious inhibitory action, wherein, in the time that concentration of sodium selenite is 20mg/L, sodium selenite conversion ratio is the highest.
The impact of table 2 concentration of sodium selenite on Lactobacillus plantarum and saccharomyces cerevisiae biomass
Equally, experiment finds that Lactobacillus plantarum and saccharomyces cerevisiae can not be degradable by sodium glutamate, and sodium glutamate can not be converted into GABA completely, in the time adding higher than 100mg/L sodium glutamate, sodium glutamate residual quantity is higher, has affected its mouthfeel as feed addictive.Therefore, sodium glutamate addition should be lower than 100mg/L.
The variation of table 3 sodium glutamate existence form in fermentation system
| Concentration of sodium glutamate | Sodium glutamate is residual | GABA |
| 20mg/L | 2.17mg/L | 4.15mg/L |
| 50mg/L | 3.03mg/L | 5.03mg/L |
| 70mg/L | 5.15mg/L | 10.8mg/L |
| 100mg/L | 9.52mg/L | 12.5mg/L |
| 150mg/L | 35mg/L | 11.17mg/L |
Concrete spray drying process is as follows: before spray drying device uses, first in equipment, pass into clear water washing, residue in equipment is washed out, after pass into 200 DEG C of above high temperature air 15-30min, after waiting equipment cooling, good homogeneous cow's milk and maltodextrin are put into charging aperture, start spraying.When spraying, setting outlet temperature is 80 DEG C, and feeding temperature is selected 120 DEG C and 100 DEG C of two temperature.Collect bacterium powder (spraying dried bacterium powder as shown in Figure 2), measure viable count with viable bacteria counting method.
Embodiment 4 mixes with other raw material
Tryptophan, amide glutaminate, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran, vitamin E are broken into 60 object particles;
Carrier zeolite powder is ground into 200 object particles;
Above-mentioned raw materials composition is weighed in the ratio in formula, is then put in the stainless steel mixer of mixture homogeneity≤5% and stirs 5-8 minute, by quantitative package finished product;
The said goods is sneaked in bacterium powder prepared by embodiment 3 according to a certain percentage, concrete adding proportion is as follows: tunning 5%-10%, tryptophan 10-25%, glutamine 15-20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1-3%, Fructus Ligustri Lucidi polysaccharide 0.1-0.2%, astragalus polyose 0.1-0.3%, pachymaran 0.1-0.2%, vitamin E 0.1-0.4%, all the other are zeolite powder.The above-mentioned additive preparing is added in piglet diet in 1% ratio, carries out feeding and management by the routine raising program of pig farm piglet.
Embodiment 5 mixes with other raw material
Scheme 1
Tunning 5%, tryptophan 10%, glutamine 15%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1%, Fructus Ligustri Lucidi polysaccharide 0.1%, astragalus polyose 0.1%, pachymaran 0.1%, vitamin E 0.1%, all the other are zeolite powder.
Scheme 2
Tunning 7.5%, tryptophan 15%, glutamine 18%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 2%, Fructus Ligustri Lucidi polysaccharide 0.1%, astragalus polyose 0.2%, pachymaran 0.1%, vitamin E 0.2%, all the other are zeolite powder.
Scheme 3
Tunning 10%, tryptophan 25%, glutamine 20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 3%, Fructus Ligustri Lucidi polysaccharide 0.2%, astragalus polyose 0.3%, pachymaran 0.2%, vitamin E 0.4%, all the other are zeolite powder.
Embodiment 6 application experiments
The above-mentioned additive preparing is added in piglet diet in 1% ratio, carries out feeding and management by the routine raising program of pig farm piglet.Additive is composed as follows: tunning 7.5%, and tryptophan 15%, glutamine 18%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 2%, Fructus Ligustri Lucidi polysaccharide 0.1%, astragalus polyose 0.2%, pachymaran 0.1%, vitamin E 0.2%, all the other are zeolite powder.
Test grouping and diet
160 healthy 28 ages in days wean castrated piglets are divided into 2 groups at random by body weight, i.e. control group, test group, establishes 10 repetitions for every group, has eachly repeated 8 pigs.Control group fed basis diet, basal diet formula is prepared with reference to bacon hogs feeding standard (2004) and U.S. NRC (2012) pig nutritional need.Test group adds in basal diet that 1% pig starter feed is anti-stress additive.
Feeding and management
Test is carried out on long-range kind of pig farm, and pig house is concrete floor.In every circle, there are a hopper and automatic drinking bowl.Pig is raised and immunity is undertaken by long-range animal husbandry pig farm Routine Management.Test piglet is chosen 28 days weanling pigs, and initial body weight is 8 kg left and right.Primitive pit forwards nursery house to and feeds 30 days, the 14:00-16:00 testing the 10th day to the 15th day observes piglet stand, lie prone sleeping, dog and sits back and waits daily behavior, within 30 days, feed and finish the mixed group of piglet (each repetition is chosen arbitrarily 4 piglets and made marks mixed crowd mutually) between rear same processed group, the daily ration of feeding is constant, adopt CCD infrared digital camera (model: SG-IR612MP, Sage, Shenzhen) Continuous Observation 8h, records in detail number of times and the time of generation fight in every hurdle piglet 8h and collects saliva and measure saliva cortisol.Eachly after off-test repeat to extract a pig and butcher and collect blood measuring blood adrenaline.
Result of the test:
The impact of each processed group piglet daily behavior is as shown in table 4.As shown in Table 4, compared with the control group of the basal diet of feeding, it is not remarkable that the dog of test group piglet sits behavior difference, but the sleeping behavior of lying prone significantly increases, the behavior of standing significantly reduces, and shows to add the anti-piglet daily behavior that stress additive of pig starter feed of the present invention and improves.
The comparison of the each processed group piglet of table 4 daily behavior
| Daily behavior | Control group | Test group |
| Lie prone sleeping | 65.08 b | 73.56 a |
| Stand | 20.23 b | 14.25 a |
| Dog sits | 3.45 | 3.51 |
With comparing in a line same factor, the female different persons of marking-up represent significant difference (P < 0.05).
Impact on fighting behavior after the mixed group of piglet is as shown in table 5.As shown in Table 5, compared with the control group of the basal diet of feeding, the time of fighting of test group piglet and the number of times of fighting all significantly reduce, and illustrates and add the anti-behavior of fighting that stress additive can significantly reduce after mixed group of piglet of pig starter feed.
The comparison of fighting behavior in 8 hours after the mixed group of the each processed group piglet of table 5
| The behavior of fighting | Control group | Test group |
| Fight the time (second) | 111.84±7.13 a | 35.89±3.09 b |
| The number of times of fighting | 15.03±0.94 a | 8.21±0.23 b |
With comparing in a line same factor, the female different persons of marking-up represent significant difference (P < 0.05).
Each processed group is as shown in table 6 on piglet saliva Determination of cortisol, the adrenergic impact of blood.As shown in Table 6, compared with the control group of the basal diet of feeding, saliva Determination of cortisol and the blood epinephrine contents of test group piglet significantly reduce, and illustrate that test adds the anti-hormone-content that stress additive can significantly reduce and stress be relevant of pig starter feed of the present invention in piglet diet.
The each processed group of table 6 is to piglet saliva Determination of cortisol, the adrenergic comparison of blood
| Index | Control group | Test group |
| Cortisol (ng/ml) | 3.13±0.05 a | 1.27±0.01 b |
| Adrenaline (pg/mL) | 112.70±1.23 a | 56.56±1.23 b |
In colleague's same factor, relatively, the female different persons of marking-up represent significant difference (P < 0.05).
Each processed group is as shown in table 7 on the impact of piglet immunological globulin content.As shown in Table 7, compared with the control group of the basal diet of feeding, immunoglobulin M in test group piglet blood (IgM) content is without significant difference, but test group piglet blood immunoglobulin A (IgA) and immunoglobulin G (IgG) content are significantly higher than control group, illustrate that in piglet diet, adding pig starter feed of the present invention resists and stress can improve piglet immunological globulin content by additive, alleviate piglet because of wean and the mixed group of pigling immunity that stress cause declines.
The comparison of the each processed group of table 7 to piglet immunological globulin content
| Index | Control group | Test group |
| IgA | 0.19±0.05 b | 0.27±0.01 a |
| IgG | 2.92±0.12 b | 3.37±0.10 a |
| IgM | 0.29±0.02 | 0.30±0.05 |
In colleague's same factor, relatively, the female different persons of marking-up represent significant difference (P < 0.05).
Although the present invention with preferred embodiment openly as above; but it is not in order to limit the present invention, any person skilled in the art, without departing from the spirit and scope of the present invention; can do various changes and modification, therefore protection scope of the present invention should be with being as the criterion that claims were defined.
Claims (8)
1. a pig starter feed is anti-stress additive, it is characterized in that, tunning 5-10%, tryptophan 10-25%, glutamine 15-20%, Radix Et Caulis Acanthopanacis Senticosi polysaccharide 1-3%, Fructus Ligustri Lucidi polysaccharide 0.1-0.2%, astragalus polyose 0.1-0.3%, pachymaran 0.1-0.2%, vitamin E 0.1-0.4%, all the other are zeolite powder; Described tunning is that agricultural wastes straw is hydrolyzed to after glucose, utilizes liquid glucose to cultivate Lactobacillus plantarum and saccharomyces cerevisiae as matrix, the bacterium powder that zymotic fluid obtains after being dried through spraying.
2. anti-described in claim 1 stress additive, it is characterized in that, described agricultural wastes straw method for hydrolysis is as follows: after crushed stalk, add water to carry out steam blasting processing according to solid-liquid ratio 1:0.5, steam blasting pretreatment pressure is 1.5-2.0MPa, and reservation pressure time is 120s; After explosion, stalk is 1:5-1:10 with the buffer solution adjusting solid-liquid ratio of pH5.0, adds pectase 50U/g, cellulase 20U/g and hemicellulase 20U/g by dry matter weight, in 40 DEG C of enzymolysis 1-3 days, adopts plate compression or centrifuge to obtain supernatant.
3. anti-described in claim 1 stress additive, it is characterized in that, described liquid glucose adds after peptone, yeast extract, diammonium hydrogen citrate, calcium salt, sylvite, magnesium salts, sodium selenite and sodium glutamate for cultivating Lactobacillus plantarum and saccharomyces cerevisiae after detoxification.
4. anti-preparation method that stress additive described in a claim 1, it is characterized in that, that agricultural wastes straw is hydrolyzed to after glucose, utilize liquid glucose to cultivate Lactobacillus plantarum and saccharomyces cerevisiae as matrix, zymotic fluid add after dry through spraying tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran, vitamin E, zeolite powder make anti-stress additive.
5. method described in claim 4, is characterized in that, is to add water to carry out steam blasting processing according to solid-liquid ratio 1:0.5 after crushed stalk, and steam blasting pretreatment pressure is at 1.5-2.0MPa, and reservation pressure time is 120s; After explosion, stalk is 1:5-1:10 with the buffer solution adjusting solid-liquid ratio of pH5.0, adds pectase 50U/g, cellulase 30U/g and hemicellulase 20U/g by dry matter weight, in 40 DEG C of enzymolysis, adopts plate compression or centrifuge to obtain supernatant; Supernatant adds after peptone, yeast extract, diammonium hydrogen citrate, calcium salt, sylvite, magnesium salts, sodium selenite and sodium glutamate for cultivating Lactobacillus plantarum and saccharomyces cerevisiae after detoxification.
6. method described in claim 4, is characterized in that, concrete steps are as follows:
1) after crushed stalk, be that 1:0.5 adds water to carry out steam blasting processing according to solid-liquid ratio, steam blasting pretreatment pressure is at 1.5-2.0MPa, and reservation pressure time is 120s;
2) after explosion, stalk is 1:5-1:10 with the buffer solution adjusting solid-liquid ratio of pH5.0, adds pectase 50U/g, cellulase 30U/g and hemicellulase 20U/g by dry matter weight, in 40 DEG C of enzymolysis 1-3 days, adopts plate compression or centrifuge to obtain supernatant;
3) liquid glucose is diluted to concentration of reduced sugar below 20% after detoxification, adds after peptone, yeast extract, diammonium hydrogen citrate, calcium salt, sylvite, magnesium salts, sodium selenite and sodium glutamate for cultivating Lactobacillus plantarum and saccharomyces cerevisiae; Wherein, viable count is 10
8cFU/mL Lactobacillus plantarum inoculum concentration is 5%, 10
7cFU/mL saccharomyces cerevisiae inoculum concentration is 1%; Cultivation temperature is 32 DEG C and cultivates 18 hours;
4) zymotic fluid adds the dry Powdered xeraphium that obtains of spraying after maltodextrin and milk powder, heating to after Lactobacillus plantarum and saccharomyces cerevisiae deactivation with tryptophan, glutamine, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, Fructus Ligustri Lucidi polysaccharide, astragalus polyose, pachymaran, vitamin E, zeolite powder make anti-stress additive.
7. method described in claim 6, it is characterized in that step 3) in add 20mg/L sodium selenite, 75mg/L sodium glutamate, 10g/L peptone, 5g/L yeast extract, 0.2g/L diammonium hydrogen citrate, 0.2g/L calcium salt, 0.1g/L sylvite and 0.1g/L magnesium salts.
8. anti-described in claim 1 stress additive, for alleviate after the mixed group of piglet stress behavior.
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| CN104905075A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing pig complete feed by solid state enzyme solution and fermentation |
| CN104905074A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing laying duck complete feed by solid state enzyme solution and fermentation |
| CN104905073A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing meat duck complete feed by solid state enzyme solution and fermentation |
| CN104905013A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing protein feed raw materials by carrying out enzymolysis and fermentation on white spirit vinasse |
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| CN104904993A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing cattle and sheep feed by solid state enzyme solution and fermentation |
| CN104920805A (en) * | 2015-06-30 | 2015-09-23 | 河南双成生物科技有限公司 | Method for producing antimicrobial-free feed through enzymosis and fermentation of straw |
| CN105076721A (en) * | 2015-08-25 | 2015-11-25 | 江西优能生物科技有限公司 | Premixing agent for reducing oxidative stress of weaned piglets and preparation method and application thereof |
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| CN113198007A (en) * | 2021-04-15 | 2021-08-03 | 河南省帮邦生物工程有限公司 | Compound preparation for clearing pig endotoxin and preparation method thereof |
| CN114532444A (en) * | 2022-03-08 | 2022-05-27 | 四川农业大学 | Fibrous thallus protein feed and preparation method thereof |
| CN114732084A (en) * | 2022-03-04 | 2022-07-12 | 扬州日兴生物科技股份有限公司 | Method for preparing anti-stress animal feed by biological processing of shrimp processing by-products |
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| CN104905075A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing pig complete feed by solid state enzyme solution and fermentation |
| CN104905074A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing laying duck complete feed by solid state enzyme solution and fermentation |
| CN104905073A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing meat duck complete feed by solid state enzyme solution and fermentation |
| CN104905013A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing protein feed raw materials by carrying out enzymolysis and fermentation on white spirit vinasse |
| CN104905018A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing protein feed material by enzymolysis and fermentation of guar meal |
| CN104904991A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing protein feed raw materials by carrying out enzymolysis and fermentation on soybean meal |
| CN104904993A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing cattle and sheep feed by solid state enzyme solution and fermentation |
| CN104920805A (en) * | 2015-06-30 | 2015-09-23 | 河南双成生物科技有限公司 | Method for producing antimicrobial-free feed through enzymosis and fermentation of straw |
| CN105076721A (en) * | 2015-08-25 | 2015-11-25 | 江西优能生物科技有限公司 | Premixing agent for reducing oxidative stress of weaned piglets and preparation method and application thereof |
| CN105076721B (en) * | 2015-08-25 | 2018-03-20 | 江西优能生物科技有限公司 | A kind of pre-mixing agent, preparation method and purposes for reducing weanling pig oxidative stress |
| CN105394413A (en) * | 2015-11-12 | 2016-03-16 | 六安瑞泰养殖科技有限公司 | Calcium-supplementing piglet feed capable of improving immunity and preparation method of calcium-supplementing piglet feed |
| CN107751612A (en) * | 2017-11-23 | 2018-03-06 | 贵州大兴农业科技发展有限公司 | A kind of feed for feeding 13 months pigs and preparation method thereof |
| CN109275781A (en) * | 2018-11-19 | 2019-01-29 | 天水市畜牧技术推广站 | It is a kind of using paper mulberry as poultry anti-stress feed of raw material and preparation method thereof |
| CN113198007A (en) * | 2021-04-15 | 2021-08-03 | 河南省帮邦生物工程有限公司 | Compound preparation for clearing pig endotoxin and preparation method thereof |
| CN114732084A (en) * | 2022-03-04 | 2022-07-12 | 扬州日兴生物科技股份有限公司 | Method for preparing anti-stress animal feed by biological processing of shrimp processing by-products |
| CN114532444A (en) * | 2022-03-08 | 2022-05-27 | 四川农业大学 | Fibrous thallus protein feed and preparation method thereof |
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