CN107484880A - A kind of Radix Codonopsis dregs of a decoction biological feed additive and preparation method and application - Google Patents
A kind of Radix Codonopsis dregs of a decoction biological feed additive and preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
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Abstract
The invention discloses a kind of Radix Codonopsis dregs of a decoction biological feed additive and preparation method and application, belong to field of feed additive technology.In order to solve the Chinese medicine slag wasting of resources, pollute environment, and the problems such as harm of abuse of the antibiotic in feed manufacturing industry and aquaculture to health of people, innovative combines medicinal fungi ganoderma lucidum and the Radix Codonopsis dregs of a decoction, using medicinal fungi ganoderma lucidum as fermented bacterium, fementative composition is collectively formed by the use of the dregs of a decoction of medicinal material Radix Codonopsis with health role as property of medicine matrix auxiliary materials such as herbage of arranging in pairs or groups again, both sides are adapted is fermented under certain condition, to produce various useful medicinal fungal substances, after last re-dry crushing feedstuff industry is can apply to as biological feed additive, aquaculture, promote animal growth, improve premunition.The invention had both solved Chinese medicine slag environmental pollution problem, can make full use of the Chinese material medicine resource of preciousness again and Chinese medicine slag is turned waste into wealth, can also reduce application of the antibiotic in aquaculture.
Description
Technical field
The present invention relates to field of feed additive technology, and in particular to a kind of Radix Codonopsis dregs of a decoction biological feed additive and its system
Preparation Method and application.
Background technology
Radix Codonopsis is Campanulaceae Radix CodonopsisCodonopsis pilosula (Franch.) Nannf., codonopsis pilosula var. modestaCodonopsis pilosula Nannf. Var. modesta (Nannf.) L. T. Shen or radix codonpsis tangshenCodonopsis tangshen Oliv. dry root, has the function that invigorating the spleen and tonifying Qi.Codonopsis pilosula polysaccharide, amino acid, glucosides,
Alkaloid, sterol etc. are its main active（Li Da, wait Review Study [J] China of the Codonopsis pilosula polysaccharides as immunologic adjuvant
Medical guide, 2013,11 (28):56-59）.Codonopsis pilosula polysaccharide can not only strengthen cellular anti-oxidant capacity, can also improve
Immunity of organisms while also there is anti-aging and antineoplastic action（Chen Jia small islands, Codonopsis pilosula polysaccharide classes are waited to exempt from tumor-bearing mice
Epidemic disease response and tumor-inhibiting action research [J] China treatment and prevention of tumour magazine, 2015,22 (17):1357-1362）.
Ganoderma lucidum (Ganoderma lucidum) seocho is also known as, for traditional Chinese medicine more than 2000 years.Research confirms spirit
Sesame not only contains polysaccharide and alkaloid, also containing furans, nucleosides and sterol isoreactivity composition（Yuan with show, wait ganoderma actives into
Point, pharmacological action and its apply China National folk medicine magazines, 2006,79:110-113）.Ganoderma lucidum can strengthen body
Immunity, improve macrophages phagocytic capacity, also there is hypotensive, antitumor and norcholesterol and low transaminase,
So as to increase CBF coronarius, promote impaired cell repair and regeneration（The rich clinical practice for send out first ganoderma lucidums and
The research Sichuan traditional Chinese medical science both at home and abroad, 2001,19 (12):17-18.）.Research shows that GL-B can suppress tumour cell life
It is long, improve immunity of organisms（Yin G,et al. Chinese herbs (Astragalus radix and Ganoderma lucidum) enhance immune response of carp, Cyprinus carpio, and protection
against Aeromonas hydrophila[J].Fish & Shellfish Immunology, 2009, 26(1):
140-145）.Compared with normal ganoderma lucidum, fermentation can improve the content of amino acid and polysaccharide in ganoderma lucidum mycelium.
Antibiotic plays an important role in the development of animal husbandry, but the abuse of antibiotic result in feed at present
The various problems such as medicament residue, drug resistance increase, environmental pollution.China produces about 210,000 tons of antibiotic per year, wherein nearly half is used for
Livestock breeding industry.The animal products of antibiotic residue brings serious harm to the health of people.In addition, China shares at present
Traditional Chinese medicine enterprise produces more than 3,000 ten thousand tons of Chinese medicine slag per year, traditional processing method is mostly burning, landfill and fixed area up to family more than 2000
Domain stacking etc., causes serious environmental pollution and the wasting of resources.To make Chinese medicine slag turn waste into wealth, during we are fermented with medicinal fungi
The dregs of a decoction, the health care biological feed additive of alternative antibiotic is researched and developed, improve livestock and poultry quality.
The content of the invention
The purpose of the present invention is that medicinal fungi and Chinese medicine slag combine, using with enhancing animal immunizing power and
The medicinal fungi glossy ganoderma fermentation Radix Codonopsis dregs of a decoction of antiviral activity have enhancing animal immunizing power to produce one kind, improve disease resistance,
The new bio feed addictive of part or all of substitute antibiotics, applied in aquaculture, makes Chinese medicine slag turn waste into wealth.
A kind of Radix Codonopsis dregs of a decoction biological feed additive and preparation method and application, the present invention are realized by following steps：
A weighs 42.50 g improvement Martin's agar medium powder, 90 DEG C of distilled water dissolving, and constant volume is sub-packed in test tube to 1 L
With 121 DEG C, sterilize 20 min, puts inclined-plane, after taking the lucidum strain that 3 test tube slants culture medium switching laboratory preserves, in 25
~ 28 DEG C of lucifuge cultures, mycelia can be used after covering with inclined-plane.
B weighs 41.5 g mould Liquid Culture original washing powders, and 90 DEG C of distilled water is dissolved, and 1.5 are added after first constant volume to 1 L
L distilled water mixes, in 10 500 mL conical flask, every bottle of 250 mL of packing, with 121 DEG C of high-pressure sterilizing pot sterilizing 20 ~
30 min, it is inoculated with the ganoderma lucidum solid slope of 3 ~ 5 pieces of soybean grain sizes after cooling in every bottle of fluid nutrient medium in superclean bench
Strain, 30 ~ 60 s are smashed to pieces respectively with tissue mashing machine with 10000 ~ 20000 rpm, with 150 ~ 200 after sterile ventilative filter membrane sealing
Rpm, 25 ~ 29 DEG C, shaking table lucifuge culture to appearance a large amount of uniform mycelium stops culture when being in exponential phase.
C takes the fresh Radix Codonopsis dregs of a decoction, measures after water content is converted to dry weight, carrys out dispensing according to following formula：The Radix Codonopsis dregs of a decoction
30 ~ 80 parts, 15 ~ 45 parts of herbage, 10 ~ 20 parts of wheat bran, 5 ~ 10 parts of corn flour, 1 ~ 2 part of lime, 1 ~ 2 part of sucrose, 1 ~ 2 part of gypsum, so
Water content is adjusted afterwards to 60% ~ 70%（w/w）, pH value 6 ~ 7 is adjusted, then with 121 DEG C of h of high-temperature sterilization 1.5 ~ 2.5, is cooled to room
Warm standby is used.
Wherein, preferable batch formula：70 ~ 80 parts of the Radix Codonopsis dregs of a decoction, 15 ~ 20 parts of herbage, 10 ~ 20 parts of wheat bran, corn flour 5 ~
10 parts, 1 ~ 2 part of lime, 1 ~ 2 part of sucrose, 1 ~ 2 part of gypsum.
Wherein, further preferred batch formula：30 ~ 40 parts of the Radix Codonopsis dregs of a decoction, 35 ~ 45 parts of herbage, 10 ~ 20 parts of wheat bran are beautiful
5 ~ 10 parts of ground rice, 1 ~ 2 part of lime, 1 ~ 2 part of sucrose, 1 ~ 2 part of gypsum.
Wherein, batch formula still more preferably：50 ~ 60 parts of the Radix Codonopsis dregs of a decoction, 20 ~ 30 parts of herbage, 10 ~ 20 parts of wheat bran,
5 ~ 10 parts of corn flour, 1 ~ 2 part of lime, 1 ~ 2 part of sucrose, 1 ~ 2 part of gypsum.
Then the lucidum strain activated in step B is aseptically inoculated into the dispensing after step C sterilizings by D,
Lucifuge culture under the conditions of 25 ~ 29 DEG C, untill mycelia is covered with, obtains tunning.
E beats powder together after the tunning of gained in step D is dried or dried, and feed addictive is made after sieving, so
Heavy metal safety detection and zoopery effect detection are carried out to feed addictive afterwards.Wherein, the tunning in step D can be with
Just used after mycelia is covered with, can continue to fermentation and reused to after producing fructification；Can also may be used with being used after drying and crushing
With fresh direct use.
, can be according to standard GB/T/T 13081-2006/5 with cold atomic absorption spectrometry after feed addictive is made
The content of feed addictive mercury in detecting step E, according to standard GB/T/T 13079-2006/7 with hydride generation atomic fluorescence light
The content of feed addictive arsenic in degree method detecting step E, according to standard GB/T/T 13080-2004 with Flame Atomic Absorption Spectrometry light
The content of feed addictive lead in spectrometry detecting step E, according to standard GB/T/T 13082-1991 with Flame Atomic Absorption Spectrometry light
The content of feed addictive cadmium in spectrometry detecting step E, according to standard GB/T/T 13885-2003 with Flame Atomic Absorption Spectrometry light
The content of feed addictive copper in spectrometry detecting step E.
By taking laying hen as an example, the using effect of feed addictive is detected.The daily management of laying hen is with reference to the feeding of common laying hen
The method of supporting, by the way of three-dimensional cage, hen house is semi open model, is divided into 3 layers of upper, middle and lower, while ensures the good ventilation of hen house
Condition.Feed base material is prepared using raw material according to corresponding nutritive proportion oneself, blank control group feeding different phase
Basal feed, experimental group different phase add 1% ~ 3% in basal feed（w/w）Feed addictive in step E.Laying hen is from small
To without using any antibiotic, observing greatly and count the upgrowth situation of chicken, the difference of resistance against diseases and egg production rate, full feeding
79 weeks cycles.As a result show, the laying hen for employing feed addictive of the invention to raise, its resistance against diseases has significant increasing
By force, and growth rate also faster.
The Radix Codonopsis dregs of a decoction biological feed additive of the present invention can be used for breeding layer chicken, increase its resistance against diseases, but not
It is limited to the cultivation of livestock and poultry, can also be applied in the cultivation of marine product, feed or premix can be used as to be used.
Embodiment
For ease of more fully understanding the present invention, it is further illustrated by the examples that follow, these embodiments belong to the present invention
Protection domain, but be not limited to protection scope of the present invention.
Embodiment 1
42.50 g improvement Martin's agar medium powder, 90 DEG C of distilled water dissolving are weighed, constant volume is sub-packed in test tube to 1 L
With 121 DEG C, sterilize 20 min, puts inclined-plane.After taking the lucidum strain that 3 test tube slants culture medium switching laboratory preserves, in 27
DEG C lucifuge culture, mycelia can be used after covering with inclined-plane.
41.5 g mould Liquid Culture original washing powders are weighed, 90 DEG C of distilled water is dissolved, and 1.5 L are added after first constant volume to 1 L
Distilled water mixes, in 10 500 mL conical flask, every bottle of 250 mL of packing.With 121 DEG C of high-pressure sterilizing pot sterilizing 20 ~
30 min.It is inoculated with the ganoderma lucidum solid slope of 3 ~ 5 pieces of soybean grain sizes after cooling in every bottle of fluid nutrient medium in superclean bench
Strain, 30 ~ 60 s are smashed to pieces respectively with tissue mashing machine with 10000 ~ 20000 rpm, with 150 ~ 200 after sterile ventilative filter membrane sealing
Rpm, 25 ~ 29 DEG C, shaking table lucifuge culture to appearance a large amount of uniform mycelium stops culture when being in exponential phase.
The fresh Radix Codonopsis dregs of a decoction are taken, measures after water content is converted to dry weight, carrys out dispensing according to following formula：The Radix Codonopsis dregs of a decoction 75
Part, 10 parts of herbage, 15 parts of wheat bran, 7 parts of corn flour, 1 part of lime, 1 part of sucrose, 1 part of gypsum.Then water content is adjusted to 65%（w/
w）, adjust pH value 6.5.Then it is standby that room temperature is cooled to dispensing 2 h of sterilizing with 121 DEG C of high-temperature steams.
Then the lucidum strain of activation is aseptically inoculated into the dispensing after sterilizing, kept away under the conditions of 27 DEG C
Optical culture, untill mycelia is covered with.
Powder is beaten together after the tunning of gained in previous step is dried or dried, and feed addictive is made after sieving, so
Heavy metal safety detection and zoopery effect detection are carried out to feed addictive afterwards.Wherein, in previous step in tunning
It can just be used after mycelia is covered with, can continue to fermentation and reused to after producing fructification；Can to be used after drying and crushing,
Direct use that can also be fresh.
The feed addictive mercury prepared is detected with cold atomic absorption spectrometry according to standard GB/T/T 13081-2006/5
Content, according to standard GB/T/T 13079-2006/7 with hydride generation atomic fluorescence photometry detect prepare feed add
The content of agent arsenic, the feed prepared is detected with flame atomic absorption spectrometry according to standard GB/T/T 13080-2004 and is added
The content of agent lead, the feed prepared is detected with flame atomic absorption spectrometry according to standard GB/T/T 13082-1991 and is added
The content of agent cadmium, the feed prepared is detected with flame atomic absorption spectrometry according to standard GB/T/T 13885-2003 and is added
The content of agent copper.
By taking laying hen as an example, the using effect of feed addictive is detected.The daily management of laying hen is with reference to the feeding of common laying hen
The method of supporting, by the way of three-dimensional cage, hen house is semi open model, is divided into 3 layers of upper, middle and lower, while ensures the good ventilation of hen house
Condition.Feed base material is prepared using raw material according to corresponding nutritive proportion oneself, blank control group feeding different phase
Basal feed, experimental group different phase add 1% ~ 3% in basal feed（w/w）Feed addictive in step 5.Laying hen is from small
To without using any antibiotic, observing greatly and count the upgrowth situation of chicken, the difference of resistance against diseases and egg production rate, full feeding
79 weeks cycles.
Embodiment 2
The batch formula of fermentation is only changed to by other operations with embodiment 1：55 parts of the Radix Codonopsis dregs of a decoction, 20 parts of herbage, 15 parts of wheat bran,
7 parts of corn flour, 1 part of lime, 1 part of sucrose, 1 part of gypsum.
Embodiment 3
The batch formula of fermentation is only changed to by other operations with embodiment 1：35 parts of the Radix Codonopsis dregs of a decoction, 40 parts of herbage, 15 parts of wheat bran,
7 parts of corn flour, 1 part of lime, 1 part of sucrose, 1 part of gypsum.
Below by taking the feed addictive prepared by embodiment 2 as an example, a huge sum of money for feed addictive prepared by the present invention is illustrated
Belong to the using effect of content and feed addictive to laying hen, other embodiment can also reach equivalent effect.
Feed addictive content of beary metal detects：
China Guangzhou Analysis ＆. Test Center of commission third party testing agency is detected to feed addictive, according to national standard
GB/T 13081-2006/5 detect the content of the feed addictive mercury prepared with cold atomic absorption spectrometry, according to national standard
GB/T 13079-2006/7 detect the content of the feed addictive arsenic prepared with hydride generation atomic fluorescence photometry, according to country
Standard GB/T 13080-2004 detect the content of the feed addictive lead prepared with flame atomic absorption spectrometry, according to country
Standard GB/T 13082-1991 detect the content of the feed addictive cadmium prepared with flame atomic absorption spectrometry, according to country
Standard GB/T 13885-2003 detect the content of the feed addictive copper prepared with flame atomic absorption spectrometry.
As a result it is as shown in table 1 below, 5 heavy metals in feed addictive：Mercury, arsenic, lead, the content of cadmium and copper are superior to country
Feed addictive content of beary metal requirement as defined in standard.
Content of beary metal in the feed addictive of table 1
Feed addictive using effect detects：
By taking laying hen as an example, the using effect of feed addictive is detected.Will be with a batch of 40 body weight 276 ± 20 g 5 week old
Laying hen（The blue ash in U.S. sea）It is randomly divided into 2 groups, respectively experimental group and blank control group.The daily management of laying hen is with reference to common
The method for breeding of laying hen, by the way of three-dimensional cage, hen house is semi open model, is divided into 3 layers of upper, middle and lower, while ensure that hen house is good
Good ventilation condition.Feed base material is prepared using raw material according to corresponding nutritive proportion oneself, and blank control group is fed not
With the basal feed in stage, experimental group different phase adds 1% ~ 3% in basal feed（w/w）The feed addictive of the present invention.
Laying hen without using any antibiotic, is observed from small to large and counts the upgrowth situation of chicken, resistance against diseases, 79 weeks full feeding cycles.
As a result show, additive is as shown in table 2 below on 150 days body weight growth rate influence statistical results of laying hen, by egg
Body weight after chicken feeds 150 days carries out statistical analysis.Compared with control group, biological feed additive of the invention can promote laying hen
Growth, difference reaches the level of signifiance（* representP<0.05,n=20）, show that this kind of biological feed additive can remarkably promote egg
Chicken grows.
Influence of the feed addictive of table 2 to laying hen body weight increase
Note：Compared with control group, " * " is representedP<0.05, significant difference.
Additive is to shown in influence statistical result table 3 of the laying hen to bird pox virus resistance, during cultivation, without using appointing
What antibiotic, is not inoculated with fowl pox vaccine, after laying hen natural infection bird pox virus, counts its self-healing situation., should compared with control group
Invention additive can significantly improve the ability of the anti-bird pox virus of laying hen, reduce case fatality rate 30%（Table 3）.
Influence of the feed addictive of table 3 to laying hen premunition
Claims (7)
1. a kind of preparation method of Radix Codonopsis dregs of a decoction biological feed additive, it is characterised in that comprise the following steps：
A：42.50 g improvement Martin's agar medium powder is weighed, is put into 90 DEG C of distilled water and dissolves, constant volume to 1 L, packing
With 121 DEG C in test tube, sterilize 20 min, puts inclined-plane, takes the Ganoderma Lucidum of 3 test tube slant culture medium switching laboratory storages
After kind, in 25 ~ 28 DEG C of lucifuge cultures, mycelia can be used after covering with inclined-plane；
B：41.5 g mould Liquid Culture original washing powders are weighed, 90 DEG C of distilled water is dissolved, and 1.5 L steamings are added after first constant volume to 1 L
Distilled water mixes, in 10 500 mL conical flask, every bottle of 250 mL of packing, with 121 DEG C of sterilizings 20 ~ 30 of high-pressure sterilizing pot
Min, it is inoculated with the ganoderma lucidum solid slope bacterium of 3 ~ 5 pieces of soybean grain sizes after cooling in every bottle of fluid nutrient medium in superclean bench
Kind, 30 ~ 60 s are smashed to pieces respectively with tissue mashing machine with 10000 ~ 20000 rpm, with 150 ~ 200 after sterile ventilative filter membrane sealing
Rpm, 25 ~ 29 DEG C, shaking table lucifuge culture to appearance a large amount of uniform mycelium stops culture when being in exponential phase；
C：The fresh Radix Codonopsis dregs of a decoction are taken, measures after water content is converted to dry weight, carrys out dispensing according to following formula：The Radix Codonopsis dregs of a decoction 30 ~
80 parts, 15 ~ 45 parts of herbage, 10 ~ 20 parts of wheat bran, 5 ~ 10 parts of corn flour, 1 ~ 2 part of lime, 1 ~ 2 part of sucrose, 1 ~ 2 part of gypsum, then
Water content is adjusted to 60% ~ 70%（w/w）, adjust pH value 6 ~ 7, then with 121 DEG C of high steams to dispensing sterilize 1.5 ~ 2.5 h,
It is standby to be cooled to room temperature；
D：Then the lucidum strain activated in step B is aseptically inoculated into the dispensing after step C sterilizings, 25 ~
Lucifuge culture under the conditions of 29 DEG C, untill mycelia is covered with, obtains tunning；
E：Powder is beaten together after the tunning of gained in step D is dried or dried, and feed addictive is made after sieving.
2. according to a kind of preparation method of Radix Codonopsis dregs of a decoction biological feed additive described in claims 1, it is characterised in that
In step C, the formula of dispensing is：70 ~ 80 parts of the Radix Codonopsis dregs of a decoction, 15 ~ 20 parts of herbage, 10 ~ 20 parts of wheat bran, 5 ~ 10 parts of corn flour,
1 ~ 2 part of lime, 1 ~ 2 part of sucrose, 1 ~ 2 part of gypsum.
3. according to a kind of preparation method of Radix Codonopsis dregs of a decoction biological feed additive described in claims 1, it is characterised in that
In step C, the formula of dispensing is：30 ~ 40 parts of the Radix Codonopsis dregs of a decoction, 35 ~ 45 parts of herbage, 10 ~ 20 parts of wheat bran, 5 ~ 10 parts of corn flour,
1 ~ 2 part of lime, 1 ~ 2 part of sucrose, 1 ~ 2 part of gypsum.
4. according to a kind of preparation method of Radix Codonopsis dregs of a decoction biological feed additive described in claims 1, it is characterised in that
In step C, the formula of dispensing is：50 ~ 60 parts of the Radix Codonopsis dregs of a decoction, 20 ~ 30 parts of herbage, 10 ~ 20 parts of wheat bran, 5 ~ 10 parts of corn flour,
1 ~ 2 part of lime, 1 ~ 2 part of sucrose, 1 ~ 2 part of gypsum.
5. according to a kind of preparation method of Radix Codonopsis dregs of a decoction biological feed additive described in claims 1, it is characterised in that
In step D, mycelia continues to reuse after fermented and cultured occurs to fructification.
A kind of 6. Radix Codonopsis dregs of a decoction biological feed additive obtained by preparation method with any one of claim 1-5.
7. Radix Codonopsis dregs of a decoction biological feed additive as claimed in claim 6 is in feed, premix and livestock and poultry and culturing marine products
In application.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850537A (en) * | 2018-05-23 | 2018-11-23 | 淮阴工学院 | The preparation method of the Chinese herbal feed additive of Chinese herbal medicine endogenetic fungus fermentation |
| KR102254820B1 (en) * | 2021-01-15 | 2021-05-24 | 주식회사 펫21 | Animal feed containing herbal medicinal materials and manufacturing method thereof |
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| CN104782905A (en) * | 2015-04-29 | 2015-07-22 | 北京联合大学 | Method for preparing feed from common andrographis herb dregs by virtue of fermentation |
| CN106615640A (en) * | 2016-09-19 | 2017-05-10 | 淄博誉江塬农业科技发展有限公司 | Biologically fermented feed and preparation method thereof |
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| CN108850537A (en) * | 2018-05-23 | 2018-11-23 | 淮阴工学院 | The preparation method of the Chinese herbal feed additive of Chinese herbal medicine endogenetic fungus fermentation |
| CN108850537B (en) * | 2018-05-23 | 2023-01-13 | 淮阴工学院 | Preparation method of Chinese herbal medicine feed additive fermented by Chinese herbal medicine endophytic fungi |
| KR102254820B1 (en) * | 2021-01-15 | 2021-05-24 | 주식회사 펫21 | Animal feed containing herbal medicinal materials and manufacturing method thereof |
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