CN105213461A - Utilize the two-way fermentation of Ganoderma-Radix Codonopsis to improve the biotechnology of its drug effect - Google Patents
Utilize the two-way fermentation of Ganoderma-Radix Codonopsis to improve the biotechnology of its drug effect Download PDFInfo
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- CN105213461A CN105213461A CN201410296194.4A CN201410296194A CN105213461A CN 105213461 A CN105213461 A CN 105213461A CN 201410296194 A CN201410296194 A CN 201410296194A CN 105213461 A CN105213461 A CN 105213461A
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Abstract
The invention discloses and utilize the two-way fermentation of Ganoderma-Radix Codonopsis to improve the biotechnology of its drug effect, comprise Radix Codonopsis pretreatment, Ganderma lucidum strain activation, the preparation of two-way fermentation medium, two-way fermentation and property of medicine change and the process of Zymocyte.Biological activity both the present invention is intended to be strengthened by two-way fermentation technique and drug effect, the antioxidant activity of Radix Codonopsis improves more than 20% and is easy to absorb after fermentation.Therefore can as building up resistance, the functional food of life lengthening and medicine, application prospect is good.
Description
Technical field
The invention belongs to bioengineering field, relating to a kind of take Chinese medicine as the two-way fermentation technique of microorganism of raw material.
Background technology
In recent years, the demand of world pharmaceutical market to conventional medicament and natural drug increases just fast, has become the new trend of world today's medicament research and development with traditional drugs and natural pharmaceutical resources exploitation original new drug and relevant health product.China is as the cradle of Chinese medicine, gather around systematic Chinese medical theory and very abundant natural pharmaceutical resources, although be faced with the favourable situation of natural drug market strong growth, internationalization paces are slow, and its basic reason is that modernization of Chinese medicine level is still lower.As Gansu has abundant medicine resource, but be generally still at present based on crude drug and elementary processed goods supply market, active constituent content is low, and value-rising is few, also cannot ensure that market is to high-end and multifarious demand; The task of top priority is the tackling of key scientific and technical problems carrying out genuine medicinal materials, improves the value of Chinese crude drug;
Radix Codonopsis
codonopisispilosula, be the dry root of Campanulaceae, have effect of spleen invigorating lung benefiting, cure mainly deficiency of the spleen and lung, anorexia and loose stool, dyspnea due to deficiency is coughed, and interior-heat such as to be quenched one's thirst the disease.Modern study shows that Radix Codonopsis has enhancing immunity, blood vessel dilating, blood pressure lowering, improves microcirculation, strengthens the effects such as hemopoietic function.In addition castering action is had to the leukopenia that chemotherapy radiotherapy causes.Radix Codonopsis main component has polysaccharide, tangshenoside, sterols, triterpenes, alkaloid, lactone, legumin constituents etc.; Ganoderma
ganodermaLucidumbe the famous and precious fungus of China's tradition, be the valuable ingredient of Chinese medicine with strengthening by means of tonics, strengthening the body resistance, clothes have effect of life lengthening for a long time; Scientific research confirm Ganoderma have improve immunity, antitumor, anticancer, ease pain, protect the liver, hypermnesis, antibacterial, antioxidation, antiinflammatory, the pharmacologically active such as antimutagenic; The auxiliary treatment etc. of Ganoderma to chronic bronchitis, hat sexually transmitted disease (STD), hepatitis, liver cirrhosis, arteriosclerosis, hyperlipidemia, hypertension, neurasthenia, oncosis has curative effect in various degree clinically; The various active material such as polysaccharide, triterpenes, furans, alkaloids, aminoacid protein class, oils, inorganic ions, sterols contained in these pharmacologically actives and Ganoderma is closely related; The demand of Ganoderma constantly increases in recent years, and wild Ganoderma is very rare, can not meet the needs of people far away, and Ganoderma Lucidum growth cycle is long; Utilize fermentation engineering can make Ganoderma raising with short production cycle, active; Namely two-way fermentation is seeded in certain substrate with certain medicinal strain, under certain environment condition, through certain hour fermentation, controls to be issued to fermentation termination and produce mycoplasma in specific quality index; Chinese medicine can be utilized as the ingredient of culture medium, build medicinal fungal substance, for break-through point is found in the combination of Chinese medicine and medicinal fungi, and exploitation has the medicine of good effects.
Summary of the invention
Chinese medicine two-way fermentation technique system adopts different Chinese crude drugs to be that substrate forms different medicinal mycoplasma, or in nutrient matrix, add the Chinese crude drug composition wholeness matrix with active component, or become multiformity (compound recipe) substrate with two or more Chinese crude drug through combined crosswise, by inoculating different strains as medicinal fungi, make the effect of fermentation from originally unidirectional only by C, the nutritional labelings such as N source, the enzyme developing into application strain makes tissue and the ingredient breakdown of medicinal mycoplasma, change, produce new composition, Fermentation is made to develop into amphicheirality, thus form new nature and flavor, the medicinal fungal substance that function is different or full property mycoplasma, therefore there is amphicheirality or multiformity, the Radix Astragali of this problem using main product in Minxian County, Gansu, as dominant fermentation substrate, with Ganoderma as strain, adopts the two-way fermentation technique of fungus to ferment, is intended to the bioactive ingredients of both increases and strengthens drug effect, especially strengthening its anti-oxidation efficacy, utilize Ganderma lucidum can multiple exoenzyme such as eccrine fiber element enzyme, peroxidase, superoxide dismutase, esterase etc. in fermentation, make it act on the Radix Astragali to improve its medicine eduction rate, increase the change of secondary metabolite, the object of the invention is to realize by following technical scheme, it is characterized in that it comprises the following steps:
(1) Radix Codonopsis pretreatment: after Radix Codonopsis clean dry, section, grinds into 4-40 order size powder;
(2) Ganderma lucidum strain activation: Linzhi is activated by activation medium, 28 DEG C of constant temperature culture 7 days; Semen Maydis powder fluid medium, 100-180r/min shaking table amplification culture 96h, treats that mycelia covers with inclined-plane for subsequent use;
(3) two-way fermentation medium preparation: through the full maize raw material 84-96% pulverized and sieved, 1% edible CaCO
3, then add the Radix Codonopsis powder of 3-15%; After above-mentioned substance mix homogeneously, add its gross weight 65-200% water and stir, pH value nature, 121 DEG C, sterilizing 30min;
(4) two-way fermentation: the Linzhi after activation is accessed above-mentioned culture medium by 12% inoculum concentration (12mL/100g), 28 degree, pH nature, relative humidity 65%, ventilate dark solid state fermentation culture 5-13d;
(5) property of medicine change: when Ganoderma covers with whole fermentation tank, and its mycelium pellet is spherical in shape, when outward appearance is dense, pure white, namely fermentation comes to an end; Before and after Radix Codonopsis fermentation, DPPH free radical scavenging activity is respectively 53.97%, 66.88%, improves 23.92% before comparatively fermenting after fermentation; Hydroxyl radical free radical clearance rate difference 40.97%, 60.68%; 48.11% is improve before comparatively fermenting after fermentation; Ganoderma Biomass >11g/100ml, mycelium pellet diameter >4.5mm; Mycelia outward appearance is dense, pure white;
(6) process of Zymocyte: under 45-65 DEG C of condition, tunning (comprising mixture and the mycoplasma of mycelia and culture matrix composition) is dried to constant weight, cross 40 mesh sieves after pulverizing, permeate is final products;
beneficial effect of the present invention
(1) technique is simple, and equipment requirements is not high, is suitable for industrialization promotion;
(2) utilize the property of medicine and functional component specific to Radix Codonopsis and Ganoderma, interacted by the profound level of the two medical health care functions such as lifting its antioxidation, free radical resisting, enhancing immunity;
(3) the above packing of product is become glossy ganoderma fermentation dry powder and oral liquid can as building up resistance, the merit medicine of slow down aging, life lengthening and functional food, application prospect is good.
Detailed description of the invention
The following examples can further illustrate the present invention, but do not limit the present invention in any way:
embodiment 1
Solid Double is to fermentation---and take drying Radix Codonopsis some, grind to form 4 order size powder by pulverizer; Take Ganoderma as fermentation medicinal fungi seed, PDA slant medium activates, and adopts Semen Maydis powder fluid medium amplification culture; By adding the Radix Codonopsis powder of 5% (accounting for the full weight amount of culture medium) concentration in the culture medium of setting, make the two-way culture medium of full property (full maize raw material 84-96%, 1% edible CaCO
3, be mass concentration).Access Ganoderma seed liquor, is placed at 28 DEG C, controls oxygen-supply quantity by force ventilation, under natural Ph condition, cultivates 5d; When Ganoderma covers with whole fermentation tank, and its mycelium pellet is spherical in shape, and when outward appearance is dense, pure white, namely fermentation comes to an end;
Preparation
Ganoderma activation medium: Semen Maydis powder 78%, wheat bran 20%, sucrose 1%, calcium sulfate 1%, pH value nature.Add its gross weight 65% water to stir, 126 DEG C of sterilizing 20min are for subsequent use;
Liquid seed culture medium: Semen Maydis powder 40g, glucose 10g, yeast extract 5g, MgSO
41.0g, VB
1these materials are added distilled water by 20-40mg/L and standardize solution is 1L, pH value nature, 121 DEG C of sterilizing 20min;
Medicine matter solid medium: 84-96% is complete, and maize raw material pulverizes and sieves, soaks 24h, the 20min that precooks to variable color, adds 1% edible CaCO
3; Add the Radix Codonopsis dry powder of 3-15%, pH value nature, 121 DEG C, sterilizing 20min.
Claims (1)
1. utilize the two-way fermentation of Ganoderma-Radix Codonopsis to improve the biotechnology of its drug effect, it is characterized in that step is as follows:
1) Radix Codonopsis pretreatment: after Radix Codonopsis clean dry, section, grinds into 4-40 order size powder;
2) Ganderma lucidum strain activation: Linzhi is activated by activation medium, 28 DEG C of constant temperature culture 7 days; Semen Maydis powder fluid medium, 100-180r/min shaking table amplification culture 96h, treats that mycelia covers with inclined-plane for subsequent use;
3) two-way fermentation medium preparation: through the full maize raw material 84-96% pulverized and sieved, 1% edible CaCO
3, then add the Radix Codonopsis powder of 3-15%; After above-mentioned substance mix homogeneously, add its gross weight 65-200% water and stir, pH value nature, 121 DEG C, sterilizing 30min;
4) two-way fermentation: the Linzhi after activation is accessed above-mentioned culture medium by 12% inoculum concentration (12mL/100g), 28 degree, pH nature, relative humidity 65%, ventilate dark solid state fermentation culture 5-13d;
5) property of medicine change: when Ganoderma covers with whole fermentation tank, and its mycelium pellet is spherical in shape, when outward appearance is dense, pure white, namely fermentation comes to an end; Before and after Radix Codonopsis fermentation, DPPH free radical scavenging activity is respectively 53.97%, 66.88%, improves 23.92% before comparatively fermenting after fermentation; Hydroxyl radical free radical clearance rate difference 40.97%, 60.68%; 48.11% is improve before comparatively fermenting after fermentation; Ganoderma Biomass >11g/100ml, mycelium pellet diameter >4.5mm; Mycelia outward appearance is dense, pure white;
6) process of Zymocyte: dried to constant weight by tunning (comprising mixture and the mycoplasma of mycelia and culture matrix composition) under 45-65 DEG C of condition, cross 40 mesh sieves after pulverizing, permeate is final products.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107484880A (en) * | 2017-10-17 | 2017-12-19 | 遵义医学院 | A kind of Radix Codonopsis dregs of a decoction biological feed additive and preparation method and application |
| CN107801987A (en) * | 2017-10-26 | 2018-03-16 | 郭景龙 | A kind of truffle and pseudo-ginseng are mycoplasma of raw material and preparation method thereof |
| CN109007790A (en) * | 2018-09-07 | 2018-12-18 | 南阳理工学院 | A kind of preparation method of Chinese yam mycoplasma powder and its Chinese yam mycoplasma powder of preparation |
| CN109528568A (en) * | 2018-12-18 | 2019-03-29 | 北京工商大学 | A kind of composition and preparation method thereof with anti-aging and white-skinned face function |
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| CN103316058A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Technology for improving drug effects of Mythic Fungus and Chinese angelica through utilizing Mythic Fungus-Chinese angelica bidirectional fermentation |
| CN103393094A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103316058A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Technology for improving drug effects of Mythic Fungus and Chinese angelica through utilizing Mythic Fungus-Chinese angelica bidirectional fermentation |
| CN103393094A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107484880A (en) * | 2017-10-17 | 2017-12-19 | 遵义医学院 | A kind of Radix Codonopsis dregs of a decoction biological feed additive and preparation method and application |
| CN107801987A (en) * | 2017-10-26 | 2018-03-16 | 郭景龙 | A kind of truffle and pseudo-ginseng are mycoplasma of raw material and preparation method thereof |
| CN109007790A (en) * | 2018-09-07 | 2018-12-18 | 南阳理工学院 | A kind of preparation method of Chinese yam mycoplasma powder and its Chinese yam mycoplasma powder of preparation |
| CN109528568A (en) * | 2018-12-18 | 2019-03-29 | 北京工商大学 | A kind of composition and preparation method thereof with anti-aging and white-skinned face function |
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