CN107801987A - A kind of truffle and pseudo-ginseng are mycoplasma of raw material and preparation method thereof - Google Patents
A kind of truffle and pseudo-ginseng are mycoplasma of raw material and preparation method thereof Download PDFInfo
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- CN107801987A CN107801987A CN201711018598.7A CN201711018598A CN107801987A CN 107801987 A CN107801987 A CN 107801987A CN 201711018598 A CN201711018598 A CN 201711018598A CN 107801987 A CN107801987 A CN 107801987A
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- China
- Prior art keywords
- ginseng
- pseudo
- truffle
- fermentation
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Links
- 241000609666 Tuber aestivum Species 0.000 title claims abstract description 67
- 244000131316 Panax pseudoginseng Species 0.000 title claims abstract description 63
- 235000003181 Panax pseudoginseng Nutrition 0.000 title claims abstract description 63
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 241000204031 Mycoplasma Species 0.000 title claims abstract description 14
- 239000002994 raw material Substances 0.000 title claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 73
- 238000000855 fermentation Methods 0.000 claims abstract description 47
- 230000004151 fermentation Effects 0.000 claims abstract description 47
- 238000000034 method Methods 0.000 claims abstract description 19
- 230000000694 effects Effects 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 34
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- 239000007788 liquid Substances 0.000 claims description 26
- 239000001888 Peptone Substances 0.000 claims description 25
- 108010080698 Peptones Proteins 0.000 claims description 25
- 235000019319 peptone Nutrition 0.000 claims description 25
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 24
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 24
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 24
- 239000001963 growth medium Substances 0.000 claims description 20
- 230000001954 sterilising effect Effects 0.000 claims description 19
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 17
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- 229930006000 Sucrose Natural products 0.000 claims description 17
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 17
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- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 16
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 16
- 239000012533 medium component Substances 0.000 claims description 16
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 16
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 16
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 16
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 8
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- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 4
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- 241000018646 Pinus brutia Species 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
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- 238000004458 analytical method Methods 0.000 claims description 3
- 235000014103 egg white Nutrition 0.000 claims description 3
- 210000000969 egg white Anatomy 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 2
- 241000233866 Fungi Species 0.000 abstract description 7
- 230000002929 anti-fatigue Effects 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
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- 230000000975 bioactive effect Effects 0.000 abstract description 2
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- 239000004615 ingredient Substances 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
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- 239000000203 mixture Substances 0.000 description 6
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 6
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 239000000306 component Substances 0.000 description 2
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- 238000001035 drying Methods 0.000 description 2
- YURJSTAIMNSZAE-HHNZYBFYSA-N ginsenoside Rg1 Chemical compound O([C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(C[C@@H]([C@H]4C(C)(C)[C@@H](O)CC[C@]4(C)[C@H]3C[C@H]2O)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)C)(C)CC1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YURJSTAIMNSZAE-HHNZYBFYSA-N 0.000 description 2
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- 230000009182 swimming Effects 0.000 description 2
- 239000001100 (2S)-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chroman-4-one Substances 0.000 description 1
- QWWVBNODQCWBAZ-WHFBIAKZSA-N (2r)-2-amino-3-[(2r)-2-carboxy-2-(methylamino)ethyl]sulfanylpropanoic acid Chemical compound CN[C@H](C(O)=O)CSC[C@H](N)C(O)=O QWWVBNODQCWBAZ-WHFBIAKZSA-N 0.000 description 1
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- QMMFVYPAHWMCMS-UHFFFAOYSA-N Dimethyl sulfide Chemical compound CSC QMMFVYPAHWMCMS-UHFFFAOYSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- QUQPHWDTPGMPEX-UHFFFAOYSA-N Hesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(COC4C(C(O)C(O)C(C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-UHFFFAOYSA-N 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- YXOLAZRVSSWPPT-UHFFFAOYSA-N Morin Chemical compound OC1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 YXOLAZRVSSWPPT-UHFFFAOYSA-N 0.000 description 1
- 241000204003 Mycoplasmatales Species 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- YURJSTAIMNSZAE-UHFFFAOYSA-N UNPD89172 Natural products C1CC(C2(CC(C3C(C)(C)C(O)CCC3(C)C2CC2O)OC3C(C(O)C(O)C(CO)O3)O)C)(C)C2C1C(C)(CCC=C(C)C)OC1OC(CO)C(O)C(O)C1O YURJSTAIMNSZAE-UHFFFAOYSA-N 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000001430 anti-depressive effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- QUQPHWDTPGMPEX-UTWYECKDSA-N aurantiamarin Natural products COc1ccc(cc1O)[C@H]1CC(=O)c2c(O)cc(O[C@@H]3O[C@H](CO[C@@H]4O[C@@H](C)[C@H](O)[C@@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)cc2O1 QUQPHWDTPGMPEX-UTWYECKDSA-N 0.000 description 1
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- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
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- APSNPMVGBGZYAJ-GLOOOPAXSA-N clematine Natural products COc1cc(ccc1O)[C@@H]2CC(=O)c3c(O)cc(O[C@@H]4O[C@H](CO[C@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)cc3O2 APSNPMVGBGZYAJ-GLOOOPAXSA-N 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 229960004544 cortisone Drugs 0.000 description 1
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- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
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- 235000019634 flavors Nutrition 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- CBEHEBUBNAGGKC-UHFFFAOYSA-N ginsenoside Rg1 Natural products CC(=CCCC(C)(OC1OC(CO)C(O)C(O)C1O)C2CCC3(C)C2C(O)CC4C5(C)CCC(O)C(C)(C)C5CC(OC6OC(CO)C(O)C(O)C6O)C34C)C CBEHEBUBNAGGKC-UHFFFAOYSA-N 0.000 description 1
- 229940025878 hesperidin Drugs 0.000 description 1
- VUYDGVRIQRPHFX-UHFFFAOYSA-N hesperidin Natural products COc1cc(ccc1O)C2CC(=O)c3c(O)cc(OC4OC(COC5OC(O)C(O)C(O)C5O)C(O)C(O)C4O)cc3O2 VUYDGVRIQRPHFX-UHFFFAOYSA-N 0.000 description 1
- QUQPHWDTPGMPEX-QJBIFVCTSA-N hesperidin Chemical compound C1=C(O)C(OC)=CC=C1[C@H]1OC2=CC(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]4[C@@H]([C@H](O)[C@@H](O)[C@H](C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-QJBIFVCTSA-N 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
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- 230000006386 memory function Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000036651 mood Effects 0.000 description 1
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 235000007708 morin Nutrition 0.000 description 1
- ARGKVCXINMKCAZ-UHFFFAOYSA-N neohesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(CO)O3)OC3C(C(O)C(O)C(C)O3)O)=CC(O)=C2C(=O)C1 ARGKVCXINMKCAZ-UHFFFAOYSA-N 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
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- 210000003296 saliva Anatomy 0.000 description 1
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- 229930182490 saponin Natural products 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Polymers & Plastics (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical fields such as medical science or health food, and in particular to a kind of truffle and pseudo-ginseng are mycoplasma of raw material and preparation method thereof, and Zymocyte of the present invention is prepared by following raw materials：Pseudo-ginseng and truffle；The present invention is used as main fermentation substrate using fresh pseudo-ginseng, strain is used as using truffle bacterium solution, fermented using fungi bi-directional fermentation technology, obtain a kind of new medicinal fungal substance preparation method, this method is intended to the content and enhancing drug effect of both bioactive ingredients of increase, can especially strengthen its anti-fatigue effect.
Description
Technical field
The invention belongs to the technical fields such as medical science or health food, and in particular to a kind of truffle and pseudo-ginseng are raw material
Mycoplasma and preparation method thereof.
Background technology
Pseudo-ginseng also known as pseudo-ginseng, the famous pharmacy man Li Shizhen (1518-1593 A.D.) of the Ming Dynasty are called " invaluable ", the Chinese patent drug " cloud for China and foreign countries of becoming famous
Southern baiyao " and " Pien Tze Huang ", i.e., be made up using pseudo-ginseng of primary raw material.Pseudo-ginseng main active is arasaponin (PNS), especially
It is that rare saponin(e Rd shows preferable pharmacological activity in treatment cardiovascular and cerebrovascular disease, anti-tumor aspect；Ginsenoside Rg1 is
One of important activity composition of pseudo-ginseng, there is antifatigue, resist oxygen lack and enhancing learning and memory function effect；Rg1 can also reduce blood
Cortisone concentration in clear, by promote BDNF, p-ERK, etc. a series of rush survivins, increase animal nerve unit generation produce
Raw antidepressant effect, loosens mood and prevention of cardiovascular disease is had great significance；
Truffle, nickname ferfas (truffle), pig arch bacterium is commonly called as, is the external of a kind of symbiosis such as with Oak Tree, pine tree, robur
VA Mycorrhizal Fungi；The truffle of France originates in Oak Tree root more, and the truffle of China originates in pine tree root, and the truffle in Yunnan is with Gaoligong Shan Mountain
For the prime quality, it is individual it is small, ugly but flavor is extremely strong, rich in a- androstanols (composition of promoting the sexual maturity) and dimethyl sulphur-based methane (the special wind of ferfas
Taste composition).A- androstanols are one of active components of truffle, are a kind of steroidal compounds, and its chemical constitution swashs with property in human body
The chemical constitution of element is similar, contains the compound in the boar saliva do not castrated, testis, liver, in the blood plasma, profuse sweating in the armpit and urine of people
In be also found；Alpha-androstanol has the effect of promoting the sexual maturity, and existing enterprise is developed into a kind of commodity --- pheromones
(pheromone) it is, very worthy；Excite brain cell activity, conditioning endocrine recent research indicate that a- androstanols also have, resist
Fatigue, antidepression etc. act on.
Applying for fermented tcm has a long history in China, is one of the important method of traditional Chinese medicine Processing methods,
Fermented tcm not only changes traditional handicraft that is pan-fried, boiling, endure, refining, steaming, soaking, and make drug effect as a kind of Preparation process technique
The raising property of medicine changes to produce noval chemical compound and reduce adverse drug and acted on.Such as：Medicated Leaven, Fermented Soybean, Rhizoma Pinelliae Fermentata etc. pass
System Chinese medicine forms by natural microbial fermentation.Traditional herb fermenting is fermented using natural microbial, and strain is impure, metabolism reason
By, effective component theory is smudgy, produce poor controllability.
The content of the invention
For these reasons, applicant passes through years of researches, using fresh pseudo-ginseng as main fermentation substrate, with truffle bacterium
Liquid is fermented using fungi bi-directional fermentation technology as strain, obtains a kind of new medicinal fungal substance preparation method, this method purport
In the content and enhancing drug effect of both bioactive ingredients of increase, it can especially strengthen its anti-fatigue effect.
What the present invention was achieved through the following technical solutions.
A kind of Zymocyte, Zymocyte are prepared by following raw materials：Pseudo-ginseng and truffle.
The preparation method of described Zymocyte includes the preparation method and bi-directional fermentation method of truffle bacterium solution, wherein two-way
Fermentation process includes：The two-way fermentation process of solid-state or liquid bi-directional fermentation method.
The preparation method of wherein truffle bacterium solution is：
A, by the fresh truffle ascocarp of collection, 25KHz ultrasonications 5min after fully being cleaned with water, then use sterilized water
Moderately ground with sterile mortar and obtain lapping liquid；
B, lapping liquid is coated on SLF culture medium flat plates, it is incubated at 23-28 DEG C, the bacterium colony grown is cultivated in SLF
Base plate streaking purifies and separates, aforesaid operations are repeated untill single bacterium colony is obtained；The SLF medium components are：Glucose
30g/L, peptone 10g/L, ammonium sulfate 1g/L, agar 20g/L, fresh pseudo-ginseng diffusion juice 1L；
C, the single bacterium colony being separated to is respectively connected to fermentation medium, in 23-28 DEG C, 150rpm shaken cultivation 6-10d, adopted
Tunning is detected with the method for efficient liquid phase chromatographic analysis, the most strong bacterial strain of production a- androstanols ability is the truffle screened
Bacterium, move it on PDA inclined-planes after cultivating, in 4 DEG C of preservations；The fermentation medium components are：Maltose 30g/L, sucrose
20g/L, peptone 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, fresh pseudo-ginseng diffusion juice 1L, 121 DEG C go out
Bacterium 20min.
D, truffle bacterium slant strains are inoculated into strain enriched medium, in 23-28 DEG C, 150rpm shaken cultivation 24h, obtained
To truffle bacterium solution；Described strain enriched medium composition is：Maltose 30g/L, sucrose 20g/L, peptone 10g/L, yeast
Cream 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, calcium chloride 1g/L, vitamin B10.01g/L, 121
DEG C sterilizing 20min；
The preparation method of wherein solid-state bi-directional fermentation is：
A, fresh pseudo-ginseng is added into nutrient solution and pure water homogenate makes the 60-70% that its water content is gross weight, in 121 DEG C
Sterilizing 20min obtains pseudo-ginseng solid medium；
B, truffle bacterium solution is inoculated into pseudo-ginseng solid medium according to 10ml/100g, being uniformly mixed makes the bed of material thick
Spend for 3-5cm, be positioned in 23-28 DEG C of incubator and ferment, fermentation ends when covering with culture medium to mycelia, by Produced by Solid-state Fermentation
Thing drying and crushing, obtains Zymocyte.
The preparation method of wherein liquid bi-directional fermentation is：
A, fresh pseudo-ginseng and pure water in mass ratio 1: 5-10 are mixed and be beaten, in 60 DEG C of adverse current extraction 1h, take filtrate
Add nutrient solution and mix, sterilize 20min in 121 DEG C, obtains pseudo-ginseng liquid culture medium；
B, 10% volume ratio truffle bacterium solution is accessed in pseudo-ginseng liquid culture medium, fermented under the conditions of 23-28 DEG C, 150rpm
6-10d；
C, after fermentation ends, zymotic fluid is extracted into 1h in normal temperature adverse current, filtrate is concentrated and dried after centrifugal filtration, sent out
Yeast-like fungi matter.
Application of the described mycoplasma in fatigue-relieving food.
Mycoplasma of the present invention is：Truffle and pseudo-ginseng bi-directional fermentation medicinal fungal substance, the mycoplasma can be separately as food
It is edible, antifatigue food consumption is used for after can also it be combined with other compositions, or carry what is obtained after mycoplasma extraction
Take thing individually or combined with other compositions and used as antifatigue food.
Nutrient solution of the present invention by deionized water, soluble starch, inorganic salts, vitamin B, ammonium nitrogen, nitrate nitrogen,
Glucose, sucrose, mannose, maltose, fructose, peptone, yeast extract, malt, wheat bran, pine needle, hesperidin, morin, first
One or more in methyllanthionine, phenylalanine, citric acid are prepared.Above-mentioned raw materials are all commercially commercially available.
Specific embodiment
Technical scheme, but protection scope of the present invention not limited to this are illustrated with specific embodiment below.
Content described in this specification embodiment is only enumerating to the way of realization of inventive concept, protection of the invention
Scope is not construed as being only limitted to the concrete form that embodiment is stated, protection scope of the present invention is also and in art technology
Personnel according to present inventive concept it is conceivable that equivalent technologies mean.Although embodiment of the invention below is retouched
State, but the invention is not limited in above-mentioned specific embodiments and applications field, following specific embodiments are only to show
It is meaning property, guiding rather than restricted.One of ordinary skill in the art is under the enlightenment of this specification and is not taking off
In the case of the scope protected from the claims in the present invention, the form of many kinds can also be made, these belong to the present invention
The row of protection.
The following experiments of the present invention, it is on the basis of multiple creative experiment, with the claimed technical scheme of the present invention
Based on, the conclusive experiment of the research staff of summary.
Test the experiment of 1 mouse anti-reflecting fatigue
Experimental animal：Kunming mouse, body weight 18-20g；
Control group：Fresh pseudo-ginseng 100g, add water 100g, be homogenized, it is standby；
Test group：Mycoplasma of the embodiment of the present invention, add water 100g, be homogenized, it is standby；
Test method：Mouse is taken, random packet, every group 10, blank group gives physiological saline 0.2ml/20g.Control group
With test group gastric infusion, dosage 10ml/kg, after being administered 1 hour, quickly mouse is put into the swimming pool got ready in advance,
Record mouse enters pond and started to the time no longer swum.It the results are shown in Table 1.
The anti-fatigue test result of table 1
| Group | Swimming time (min) |
| Blank group | 2.2±0.8 |
| Control group | 4.9±0.7* |
| 1 group of embodiment | 8.2±1.0**# |
| 2 groups of embodiment | 8.1±0.9**# |
| 3 groups of embodiment | 7.9±0.7**# |
| 4 groups of embodiment | 8.0±1.2**# |
| 5 groups of embodiment | 7.7±0.6**# |
| 6 groups of embodiment | 8.1±0.7**# |
| 7 groups of embodiment | 8.0±1.1**# |
| 8 groups of embodiment | 8.3±0.7**# |
| 9 groups of embodiment | 9.1±1.2**# |
| 10 groups of embodiment | 9.4±1.0**# |
| 11 groups of embodiment | 9.3±0.7**# |
| 12 groups of embodiment | 9.0±1.1**# |
Note：* * p ＜ 0.01, the #p ＜ 0.05 compared with control group compared with control group.
Conclusion (of pressure testing)：Above-mentioned experiment shows that mycoplasma of the present invention has good antifatigue effect, in dosage identical feelings
Under condition, compare than pseudo-ginseng group with significant difference (P<0.05).
Prepare embodiment
Embodiment 1
The preparation method of truffle bacterium solution is：
A, by the fresh truffle ascocarp of collection, 25KHz ultrasonications 5min after fully being cleaned with water, then use sterilized water
Moderately ground with sterile mortar and obtain lapping liquid；
B, lapping liquid is coated on SLF culture medium flat plates, it is incubated at 23 DEG C, to the bacterium colony that grows in SLF culture mediums
Plate streaking purifies and separates, aforesaid operations are repeated untill single bacterium colony is obtained；The SLF medium components are：Glucose
30g/L, peptone 10g/L, ammonium sulfate 1g/L, agar 20g/L, fresh pseudo-ginseng diffusion juice 1L；
C, the single bacterium colony being separated to is respectively connected to fermentation medium, in 23 DEG C, 150rpm shaken cultivation 6d, using efficient
The method detection tunning of liquid-phase chromatographic analysis, the most strong bacterial strain of production a- androstanols ability is the truffle bacterium screened, by it
Move on PDA inclined-planes after cultivating, in 4 DEG C of preservations；The fermentation medium components are：Maltose 30g/L, sucrose 20g/L, albumen
Peptone 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, fresh pseudo-ginseng diffusion juice 1L, 121 DEG C of sterilizing 20min.
D, truffle bacterium slant strains 20g is inoculated into 200ml strain enriched mediums, in 23 DEG C, 150rpm shaken cultivations
24h, obtain truffle bacterium solution 180ml；Described strain enriched medium composition is：Maltose 30g/L, sucrose 20g/L, peptone
10g/L, yeast extract 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, calcium chloride 1g/L, vitamin B1
0.01g/L, 121 DEG C of sterilizing 20min；
Process for solid state fermentation：
Fresh pseudo-ginseng 100g addition nutrient solution 5g and pure water are homogenized to make its water content be gross weight 60% A, in
121 DEG C of sterilizing 20min obtain pseudo-ginseng solid medium；
B, truffle bacterium solution 10ml is inoculated into 100g pseudo-ginseng solid mediums, being uniformly mixed makes the thickness of feed layer be
3cm, it is positioned in 23 DEG C of incubator and ferments, fermentation ends when covering with culture medium to mycelia, by product by solid-state fermentation xeraphium
It is broken, obtain Zymocyte 90g.
Embodiment 2
The preparation method of truffle bacterium solution is：
A, by the fresh truffle ascocarp of collection, 25KHz ultrasonications 5min after fully being cleaned with water, then use sterilized water
Moderately ground with sterile mortar and obtain lapping liquid；
B, lapping liquid is coated on SLF culture medium flat plates, it is incubated at 23 DEG C, to the bacterium colony that grows in SLF culture mediums
Plate streaking purifies and separates, aforesaid operations are repeated untill single bacterium colony is obtained；The SLF medium components are：Glucose
30g/L, peptone 10g/L, ammonium sulfate 1g/L, agar 20g/L, fresh pseudo-ginseng diffusion juice 1L；
C, the single bacterium colony being separated to is respectively connected to fermentation medium, in 23 DEG C, 150rpm shaken cultivation 6d, using efficient
The method detection tunning of liquid-phase chromatographic analysis, the most strong bacterial strain of production a- androstanols ability is the truffle bacterium screened, by it
Move on PDA inclined-planes after cultivating, in 4 DEG C of preservations；The fermentation medium components are：Maltose 30g/L, sucrose 20g/L, albumen
Peptone 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, fresh pseudo-ginseng diffusion juice 1L, 121 DEG C of sterilizing 20min.
D, truffle bacterium slant strains 20g is inoculated into 200ml strain enriched mediums, in 23 DEG C, 150rpm shaken cultivations
24h, obtain truffle bacterium solution 180ml；Described strain enriched medium composition is：Maltose 30g/L, sucrose 20g/L, peptone
10g/L, yeast extract 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, calcium chloride 1g/L, vitamin B1
0.01g/L, 121 DEG C of sterilizing 20min；
The preparation method of liquid bi-directional fermentation is：
A, fresh pseudo-ginseng 100g is mixed and is beaten with pure water 500, in 60 DEG C of adverse current extraction 1h, take filtrate to add nutrition
Liquid 5g is simultaneously mixed, and sterilize 20min in 121 DEG C, obtains pseudo-ginseng liquid culture medium；
B, truffle bacterium solution 50ml is accessed in 500ml pseudo-ginseng liquid culture mediums, ferment 6d under the conditions of 23 DEG C, 150rpm；
C, after fermentation ends, zymotic fluid is extracted into 1h in normal temperature adverse current, filtrate is concentrated and dried after centrifugal filtration, sent out
Yeast-like fungi matter 60g.
Embodiment 3
The preparation method of truffle bacterium solution is：
A, by the fresh truffle ascocarp of collection, 25KHz ultrasonications 5min after fully being cleaned with water, then use sterilized water
Moderately ground with sterile mortar and obtain lapping liquid；
B, lapping liquid is coated on SLF culture medium flat plates, it is incubated at 28 DEG C, to the bacterium colony that grows in SLF culture mediums
Plate streaking purifies and separates, aforesaid operations are repeated untill single bacterium colony is obtained；The SLF medium components are：Glucose
30g/L, peptone 10g/L, ammonium sulfate 1g/L, agar 20g/L, fresh pseudo-ginseng diffusion juice 1L；
C, the single bacterium colony being separated to is respectively connected to fermentation medium, in 28 DEG C, 150rpm shaken cultivation 10d, using height
The method detection tunning of effect liquid phase chromatogram analysis, the most strong bacterial strain of production a- androstanols ability is the truffle bacterium screened, will
It is moved on PDA inclined-planes after culture, in 4 DEG C of preservations；The fermentation medium components are：Maltose 30g/L, sucrose 20g/L, egg
White peptone 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, fresh pseudo-ginseng diffusion juice 1L, 121 DEG C of sterilizing 20min.
D, truffle bacterium slant strains 20g is inoculated into 200ml strain enriched mediums, in 28 DEG C, 150rpm shaken cultivations
24h, obtain truffle bacterium solution 180ml；Described strain enriched medium composition is：Maltose 30g/L, sucrose 20g/L, peptone
10g/L, yeast extract 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, calcium chloride 1g/L, vitamin B1
0.01g/L, 121 DEG C of sterilizing 20min；
Process for solid state fermentation：
Fresh pseudo-ginseng 100g addition nutrient solution 10g and pure water are homogenized to make its water content be gross weight 70% A, in
121 DEG C of sterilizing 20min obtain pseudo-ginseng solid medium；
B, truffle bacterium solution 10ml is inoculated into 100g pseudo-ginseng solid mediums, being uniformly mixed makes the bed of material thick
8 degree are 5cm, are positioned in 28 DEG C of incubator and ferment, fermentation ends when covering with culture medium to mycelia, solid-state are sent out
Ferment product drying and crushing, obtains Zymocyte 92g.
Embodiment 4
The preparation method of truffle bacterium solution is：
A, by the fresh truffle ascocarp of collection, 25KHz ultrasonications 5min after fully being cleaned with water, then use sterilized water
Moderately ground with sterile mortar and obtain lapping liquid；
B, lapping liquid is coated on SLF culture medium flat plates, it is incubated at 28 DEG C, to the bacterium colony that grows in SLF culture mediums
Plate streaking purifies and separates, aforesaid operations are repeated untill single bacterium colony is obtained；The SLF medium components are：Glucose
30g/L, peptone 10g/L, ammonium sulfate 1g/L, agar 20g/L, fresh pseudo-ginseng diffusion juice 1L；
C, the single bacterium colony being separated to is respectively connected to fermentation medium, in 28 DEG C, 150rpm shaken cultivation 10d, using height
The method detection tunning of effect liquid phase chromatogram analysis, the most strong bacterial strain of production a- androstanols ability is the truffle bacterium screened, will
It is moved on PDA inclined-planes after culture, in 4 DEG C of preservations；The fermentation medium components are：Maltose 30g/L, sucrose 20g/L, egg
White peptone 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, fresh pseudo-ginseng diffusion juice 1L, 121 DEG C of sterilizing 20min.
D, truffle bacterium slant strains 20g is inoculated into 200ml strain enriched mediums, in 28 DEG C, 150rpm shaken cultivations
24h, obtain truffle bacterium solution 180ml；Described strain enriched medium composition is：Maltose 30g/L, sucrose 20g/L, peptone
10g/L, yeast extract 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, calcium chloride 1g/L, vitamin B1
0.01g/L, 121 DEG C of sterilizing 20min；
The preparation method of liquid bi-directional fermentation is：
A, fresh pseudo-ginseng 100g is mixed and is beaten with pure water 1000ml, in 60 DEG C of adverse current extraction 1h, take filtrate to add
Nutrient solution 20g is simultaneously mixed, and sterilize 20min in 121 DEG C, obtains pseudo-ginseng liquid culture medium；
B, truffle bacterium solution 100ml is accessed in 1000ml pseudo-ginseng liquid culture mediums, fermented under the conditions of 28 DEG C, 150rpm
10d；
C, after fermentation ends, zymotic fluid is extracted into 1h in normal temperature adverse current, filtrate is concentrated and dried after centrifugal filtration, sent out
Yeast-like fungi matter 63g.
Embodiment 5
The preparation method of truffle bacterium solution is：
A, by the fresh truffle ascocarp of collection, 25KHz ultrasonications 5min after fully being cleaned with water, then use sterilized water
Moderately ground with sterile mortar and obtain lapping liquid；
B, lapping liquid is coated on SLF culture medium flat plates, it is incubated at 25 DEG C, to the bacterium colony that grows in SLF culture mediums
Plate streaking purifies and separates, aforesaid operations are repeated untill single bacterium colony is obtained；The SLF medium components are：Glucose
30g/L, peptone 10g/L, ammonium sulfate 1g/L, agar 20g/L, fresh pseudo-ginseng diffusion juice 1L；
C, the single bacterium colony being separated to is respectively connected to fermentation medium, in 25 DEG C, 150rpm shaken cultivation 8d, using efficient
The method detection tunning of liquid-phase chromatographic analysis, the most strong bacterial strain of production a- androstanols ability is the truffle bacterium screened, by it
Move on PDA inclined-planes after cultivating, in 4 DEG C of preservations；The fermentation medium components are：Maltose 30g/L, sucrose 20g/L, albumen
Peptone 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, fresh pseudo-ginseng diffusion juice 1L, 121 DEG C of sterilizing 20min.
D, truffle bacterium slant strains 20g is inoculated into 200ml strain enriched mediums, in 25 DEG C, 150rpm shaken cultivations
24h, obtain truffle bacterium solution 180ml；Described strain enriched medium composition is：Maltose 30g/L, sucrose 20g/L, peptone
10g/L, yeast extract 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, calcium chloride 1g/L, vitamin B1
0.01g/L, 121 DEG C of sterilizing 20min；
The preparation method of solid-state bi-directional fermentation is：
Fresh pseudo-ginseng 100g addition nutrient solution 3g and pure water are homogenized to make its water content be gross weight 65% A, in
121 DEG C of sterilizing 20min obtain pseudo-ginseng solid medium；
B, truffle bacterium solution 10ml is inoculated into 100g pseudo-ginseng solid mediums, being uniformly mixed makes the thickness of feed layer be
4cm, it is positioned in 25 DEG C of incubator and ferments, fermentation ends when covering with culture medium to mycelia, by product by solid-state fermentation xeraphium
It is broken, obtain Zymocyte 95g.
Embodiment 6
The preparation method of truffle bacterium solution is：
A, by the fresh truffle ascocarp of collection, 25KHz ultrasonications 5min after fully being cleaned with water, then use sterilized water
Moderately ground with sterile mortar and obtain lapping liquid；
B, lapping liquid is coated on SLF culture medium flat plates, it is incubated at 25 DEG C, to the bacterium colony that grows in SLF culture mediums
Plate streaking purifies and separates, aforesaid operations are repeated untill single bacterium colony is obtained；The SLF medium components are：Glucose
30g/L, peptone 10g/L, ammonium sulfate 1g/L, agar 20g/L, fresh pseudo-ginseng diffusion juice 1L；
C, the single bacterium colony being separated to is respectively connected to fermentation medium, in 25 DEG C, 150rpm shaken cultivation 8d, using efficient
The method detection tunning of liquid-phase chromatographic analysis, the most strong bacterial strain of production a- androstanols ability is the truffle bacterium screened, by it
Move on PDA inclined-planes after cultivating, in 4 DEG C of preservations；The fermentation medium components are：Maltose 30g/L, sucrose 20g/L, albumen
Peptone 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, fresh pseudo-ginseng diffusion juice 1L, 121 DEG C of sterilizing 20min.
D, truffle bacterium slant strains 20g is inoculated into 200ml strain enriched mediums, in 25 DEG C, 150rpm shaken cultivations
24h, obtain truffle bacterium solution 180ml；Described strain enriched medium composition is：Maltose 30g/L, sucrose 20g/L, peptone
10g/L, yeast extract 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, calcium chloride 1g/L, vitamin B1
0.01g/L, 121 DEG C of sterilizing 20min；
The preparation method of liquid bi-directional fermentation is：
A, fresh pseudo-ginseng 100g is mixed and is beaten with pure water 600ml, in 60 DEG C of adverse current extraction 1h, take filtrate to add battalion
Nutrient solution 3g is simultaneously mixed, and sterilize 20min in 121 DEG C, obtains pseudo-ginseng liquid culture medium；
B, truffle bacterium solution 60ml is accessed in 600ml pseudo-ginseng liquid culture mediums, ferment 8d under the conditions of 25 DEG C, 150rpm；
C, after fermentation ends, zymotic fluid is extracted into 1h in normal temperature adverse current, filtrate is concentrated and dried after centrifugal filtration, sent out
Yeast-like fungi matter 66g.
Claims (6)
1. a kind of Zymocyte, it is characterised in that Zymocyte is prepared by following raw materials：Pseudo-ginseng and truffle.
2. a kind of Zymocyte according to claim 1, it is characterised in that the preparation method of Zymocyte includes truffle bacterium
The preparation method and bi-directional fermentation method of liquid, wherein bi-directional fermentation method include：The two-way fermentation process of solid-state or the two-way hair of liquid
Fermenting process.
3. a kind of mycoplasma according to claim 2, the preparation method of wherein truffle bacterium solution are：
A, by the fresh truffle ascocarp of collection, 25KHz ultrasonications 5min after fully being cleaned with water, then with sterilized water and nothing
Bacterium mortar moderately grinds and obtains lapping liquid；
B, lapping liquid is coated on SLF culture medium flat plates, it is incubated at 23-28 DEG C, the bacterium colony grown is put down in SLF culture mediums
Plate line purifies and separates, repeat aforesaid operations untill single bacterium colony is obtained；The SLF medium components are：Glucose 30g/
L, peptone 10g/L, ammonium sulfate 1g/L, agar 20g/L, fresh pseudo-ginseng diffusion juice 1L；
C, the single bacterium colony being separated to is respectively connected to fermentation medium, in 23-28 DEG C, 150rpm shaken cultivation 6-10d, using height
The method detection tunning of effect liquid phase chromatogram analysis, the most strong bacterial strain of production a- androstanols ability is the truffle bacterium screened, will
It is moved on PDA inclined-planes after culture, in 4 DEG C of preservations；The fermentation medium components are：Maltose 30g/L, sucrose 20g/L, egg
White peptone 10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, fresh pseudo-ginseng diffusion juice 1L, 121 DEG C of sterilizing 20min.
D, truffle bacterium slant strains are inoculated into strain enriched medium, in 23-28 DEG C, 150rpm shaken cultivation 24h, obtain pine
Reveal bacterium solution；Described strain enriched medium composition is：Maltose 30g/L, sucrose 20g/L, peptone 10g/L, yeast extract
10g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, ammonium sulfate 1g/L, calcium chloride 1g/L, vitamin B10.01g/L, 121 DEG C
Sterilize 20min.
4. a kind of mycoplasma according to claim 2, the preparation method of wherein solid-state bi-directional fermentation are：
A, fresh pseudo-ginseng is added into nutrient solution and pure water homogenate makes the 60-70% that its water content is gross weight, in 121 DEG C of sterilizings
20min obtains pseudo-ginseng solid medium；
B, truffle bacterium solution is inoculated into pseudo-ginseng solid medium according to 10ml/100g, being uniformly mixed makes the thickness of feed layer be
3-5cm, it is positioned in 23-28 DEG C of incubator and ferments, fermentation ends when covering with culture medium to mycelia, product by solid-state fermentation is done
Dry crushing, obtains Zymocyte.
5. a kind of mycoplasma according to claim 2, the preparation method of wherein liquid bi-directional fermentation are：
A, fresh pseudo-ginseng and pure water in mass ratio 1: 5-10 are mixed and be beaten, in 60 DEG C of adverse current extraction 1h, take filtrate to add
Nutrient solution simultaneously mixes, and sterilize 20min in 121 DEG C, obtains pseudo-ginseng liquid culture medium；
B, 10% volume ratio truffle bacterium solution is accessed in pseudo-ginseng liquid culture medium, ferment 6- under the conditions of 23-28 DEG C, 150rpm
10d；
C, after fermentation ends, zymotic fluid is extracted into 1h in normal temperature adverse current, filtrate is concentrated and dried after centrifugal filtration, obtains zymophyte
Matter.
6. according to a kind of mycoplasma described in claim any one of 1-6, it is characterised in that described mycoplasma is in fatigue-relieving food
In application.
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