CN108159291A

CN108159291A - Plant extracts and probiotics compounding product and preparation method thereof

Info

Publication number: CN108159291A
Application number: CN201711287884.3A
Authority: CN
Inventors: 韩大春; 韩淑艳
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-12-07
Filing date: 2017-12-07
Publication date: 2018-06-15

Abstract

The present invention relates to a Plant Extracts and probiotics to compound product, compound probiotic and plant extracts is re-dubbed, compound probiotic matches composition by weight percent including following components：Lactobacillus plantarum 9%, Bifidobacterium Bifidum 8%, lactobacillus acidophilus 4%, Lactobacillus rhamnosus 4%, Lactobacillus helveticus 4%, lactobacillus bulgaricus 4%, Lactobacillus paracasei 4%；Plant extracts matches composition by weight percent including following components：Rhizoma imperatae 7%, corn stigma 8%, Rhizoma Et Radix Notopterygii 7%, Radix Angelicae Sinensis 7%, Poria cocos 7%, radix cyathulae 7%, resveratrol 10%, sargentodoxa cuneata 5%, honeysuckle 5%.The beneficial effects of the present invention are inventive article it is efficient, safe, harmless, good mouthfeel, can take for a long time, solve the problems, such as that function is single in existing product, effect is general, palliative, inconvenient to use.

Description

Plant extracts and probiotics compounding product and preparation method thereof

Technical field

It is specifically a kind of the present invention relates to liver protection, shield kidney and alleviation, the preparing technical field for treating gout product Plant extracts and probiotics compounding product and preparation method thereof.

Background technology

It is higher with meat, aquatic product, soybean product content containing different purine in the food of our daily ingestions, And vegetable and fruit class content is relatively fewer, in the synthesis and decomposable process of purine, there are many participations of enzyme, congenital due to enzyme Sexual abnormality or certain not yet specific factor human body metabolisms get muddled, and the synthesis of uric acid is made to increase or discharge and is reduced, as a result Hyperuricemia can be caused, this people is mostly related with heredity, and when serum Uric Acid Concentration is excessively high, uric acid is sunk in the form of sodium salt Product causes the foreign matter inflammatory reaction of tissue, into the seed of trouble for causing gout in joint, soft tissue, cartilage and kidney.Nearly more than ten Nian Lai, the higher people of blood uric acid is more and more, and the people of goat is also more and more, causes more high lithemias and gout patients Reason is incessantly hereditary, scientific investigations showed that, the main reason is that, excessive diet causes kidney can't bear the heavy load, finally, kidney excretion The function reduction of uric acid so that caused by uric acid is hoarded in human body.Purine nucleotides catabolic reaction basic process is nucleosides Acid is ultimately breaks down into uric acid under the action of nucleotidase, external with urine ejection.Xanthine oxidase is important in catabolism Enzyme.Purine nucleotides catabolism mainly carries out in liver, small intestine and kidney, purine metabolic disturbance：Uric acid excessively causes gout Disease, uric acid content liter in patient's blood, thus, the people of hepatic and renal function exception, it is easier to gout must be gone up, eaten excessively when treating gout Medicine not only without effect, can aggravate gout instead.

Probiotics refers to improve the active bacteria formulation and its metabolite of host health level and health status；Have in animal body The bacterium or fungi of benefit mainly have：Lactic acid bacteria, Bifidobacterium, actinomyces, saccharomycete etc..The function of studying in the world at present is most strong Big product be mainly above all kinds of microorganism groups into composite reactive probiotics；Prebiotic fungus strain is a kind of to the thin of animal health Bacterium, they can direct catalysis polymer substance.

Enzyme is the large biological molecule for having biocatalytic Activity, i.e. biocatalyst, it can accelerate the speed of biochemical reaction Degree, but do not change direction and the product of reaction.But unlike other abiotic catalyst, enzyme has the single-minded of height Property, only catalysis specifically reacts or generates specific configuration.Thus single enzyme component is single, can only be catalyzed big point of a certain biology Son is not particularly suited for diversified large biological molecule substrate, old friends in living nature and complex enzyme is conducted in-depth research, It is developed and is increasingly paid close attention to.In the catalystic converter system of enzyme, reactant molecule is referred to as substrate, the catalysis that substrate passes through enzyme Another molecule is converted into, almost all of cellular activity process is required for the participation organism of enzyme by cell composition, Mei Gexi Since the presence of enzyme just shows various vital movements, internal metabolism could carry out born of the same parents.Enzyme is metabolic in human body Catalyst, only enzyme exists, and items biochemical reaction could be carried out in human body.Human enzymes are more, more complete, and life is got over Health.When also just terminating without organized enzyme, life in human body.Human diseases, it is most of with azymia or dyssynthesis It is related.

For this purpose, we set about from the formula of product, efficient, safe, harmless, good mouthfeel, the plant that can be taken for a long time are developed Object extract compounds product with probiotics.

Invention content

The defects of the purpose of the present invention is overcoming the prior art, provides a Plant Extracts and compounds product with probiotics, The present invention therefrom preferably goes out that effect is best by the domestication culture to tens kinds of probiotics, and be most suitable for human body application 7 kinds are prebiotic Bacterium carries out compatibility, screening, the experiment of more than 100 kind plants, final compounding product should achieve the effect that it is best, again using medicine Homologous plant non-toxic to humans is eaten, so as to which the plant that obtains efficient, safe, harmless, good mouthfeel, can take for a long time carries Object and probiotics is taken to compound product, solves that function in existing product is single, effect is general, palliative, inconvenient to use asks Topic.

The invention is realized by the following technical scheme：

One Plant Extracts compound product with probiotics, and compound probiotic and plant extracts are re-dubbed, wherein, it is multiple It closes probiotics and matches composition by weight percent including following components：Lactobacillus plantarum 9%, Bifidobacterium Bifidum 8%, lactobacillus acidophilus 4%th, Lactobacillus rhamnosus 4%, Lactobacillus helveticus 4%, lactobacillus bulgaricus 4%, Lactobacillus paracasei 4%；Plant extract Object matches composition by weight percent including following components：Rhizoma imperatae 7%, corn stigma 8%, Rhizoma Et Radix Notopterygii 7%, Radix Angelicae Sinensis 7%, Poria cocos 7%, radix cyathulae 7%, resveratrol 10%, sargentodoxa cuneata 5%, honeysuckle 5%.

The invention solves another technical problem be to provide the system of above-mentioned plant extracts and probiotics compounding product Preparation Method is as follows：

Step (1), lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus helveticus, Bao Jiali Sub- lactobacillus, Lactobacillus paracasei and saccharomyces cerevisiae stock, ferment through single strain and are mixed, and obtain zymocyte liquid, fermentation With the control of culture medium initial ph value in 4-8, cultivation temperature is controlled at 30 DEG C to 50 DEG C, and incubation time is with fermentation termination pH value to 3- Subject to 6；

Step (2) after each strain fermentation, takes whole zymotic fluids to be centrifuged, concentrated, and takes the bacterium obtained after concentration Mud adds in protective agent by the 5%-15% of bacterium mud mass ratio, is freeze-dried or is spray-dried after emulsification uniformly, made highly concentrated Spend active microbe powder or probiotics powder；

Step (3), in the lab carries out obtained each kind bacterium powder the inspection of technical indicator, and technical indicator includes According to the bacteria containing amount of recipe requirements in water content, miscellaneous bacteria infection and every gram of bacterium powder；

Step (4), by lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Switzerland obtained by step (2) Each viable bacteria powder of lactobacillus, lactobacillus bulgaricus, Lactobacillus paracasei is according to aforementioned weight than carrying out dispensing mixing, viable count Control is 10⁶-10⁹CFU/ grams, it is prepared into complex solid powder；

Step (5), the preparation method of plant extracts specifically include each plant material stock, clean, drying, grinding is beaten Slurry, water extraction, extracting solution refined filtration are concentrated under reduced pressure, liquid extract, and extraction is stood still for crystals, and is filtered, coarse crystallization, dissolving, degreasing decoloring, Condensing crystallizing, recrystallization, coarse-grain, condensing crystallizing is dry, is sieved and obtains extract, and the withdrawal ratio of raw material and extract is 20： 1。

Step (6) is carried out step (4) complex solid powder, step (5) extract and resveratrol by aforementioned weight Dispensing mixes, and is performed by GMP working specifications, and operating environment humidity is less than 30%, is sufficiently mixed to obtain product.

Preferably, the protective agent is powdered glucose, cane sugar powder, whey powder, lactose powder, serum powder, trehalose, glutamic acid Any one in sodium, glycerol yeast powder, maltodextrin or arbitrary several mixing.

The beneficial effects of the present invention are：

Inventive article enters purine human body with bidirectional modulation and decomposition, and probiotics is by institute's purine-containing in food It is decomposed when passing through intestinal absorption, enhancing body quickly removes most purine, and by probiotics in enteron aisle The polypeptide for generating and being largely beneficial to health is colonized, blood fat is high because gout patients largely have, high blood pressure, the spies such as blood glucose height Point, the present invention, which also takes into account, adjusts blood pressure, blood glucose, blood fat and other effects.In addition produced by the present invention in the plant extracts of compatibility Distinctive plant alkaloid, the product of purine, uric acid for the symptom of first gout, can such as suffer from effectively comprehensive and blood Locate pain, redness, have analgesic antiphlogistic, detumescence effect, for severe gout patient articular position, ligament tissue accumulates a large amount of gouts The patient of stone also has the effect for dissolving tophus in addition to above-mentioned function.

Lactobacillus helveticus in the present invention is capable of a variety of biologically active peptides beneficial to human body of lactoalbumin hydrolysate generation, tool There is the angiotoninase that inhibition causes hypertension, active effect blood pressure reduction effect has improvement sleep and increases blood calcium concentration Effect；Lactobacillus plantarum has certain immunoregulation effect, there is inhibiting effect to pathogenic bacteria, reduce serum cholesterol content and Prevention cardiovascular and cerebrovascular is cured the disease, and intestinal flora balance is maintained to promote absorption of nutrient ingredients, alleviates lactose intolerance, inhibits tumour thin The formation of born of the same parents；Lactobacillus bulgaricus can adjust intestinal flora balance, improve constipation, reduce cholesterol, improve liver function, control Vivotoxin, the growth for inhibiting spoilage organisms in enteron aisle, manufacture nutriment, the development for stimulating healthy cell.

Specific embodiment

Illustrate this patent with example below, be then tested for the property.It should be noted that the embodiments described below It is exemplary, is only used for explaining the present invention, and it is not intended that limitation to this patent.

One Plant Extracts compound product with probiotics, and compound probiotic and plant extracts are re-dubbed, wherein, it is multiple It closes probiotics and includes following components：Lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus helveticus, Lactobacillus bulgaricus, Lactobacillus paracasei；Plant extracts matches composition by weight percent including following components：Cogongrass Root, corn stigma, Rhizoma Et Radix Notopterygii, Radix Angelicae Sinensis, Poria cocos, radix cyathulae, sargentodoxa cuneata, honeysuckle, resveratrol.

Bifidobacterium：Treatment chronic diarrhea, which improves intestinal environment, can simultaneously treat because largely using antibiotic；

Lactobacillus paracasei：It can promote the balance of body microorganism species and enzyme and the immunologic mechanism of stimulation specificity It can play prevention disease, development is promoted to build up health, slow down aging and extend the effect in service life；

Lactobacillus acidophilus：Inhibit the increment of enteron aisle undesirable microorganism has antagonism to pathogenic microorganisms；

Lactobacillus rhamnosus：Balance improves function of intestinal canal, enhances human autoimmune's ability, promotes Bifidobacterium and acidophilus The growth of lactobacillus, prevention help to treat diarrhea, preventing respiratory tract infection, exclude toxin, is pre- anti-caries, pre- hypo-allergenic.

Lactobacillus helveticus：It is capable of a variety of biologically active peptides beneficial to human body of lactoalbumin hydrolysate generation, has and inhibit to draw The angiotoninase of hypertension is played, active effect blood pressure reduction effect plays the role of improving sleep and increases blood calcium concentration；

Lactobacillus plantarum：There is certain immunoregulation effect, there is inhibiting effect to pathogenic bacteria, reduce serum cholesterol content It cures the disease with prevention cardiovascular and cerebrovascular, intestinal flora balance is maintained to promote absorption of nutrient ingredients, alleviate lactose intolerance, inhibit tumour The formation of cell；Inhibit the growth of spoilage organisms in enteron aisle, manufacture nutriment, the development for stimulating healthy cell.

Lactobacillus bulgaricus：Intestinal flora balance is adjusted, improves constipation, reduces cholesterol, improve liver function, inhibit body Endotoxin is grown.

Rhizoma imperatae：Pyreticosis polydipsia is controlled in cool blood, hemostasis, heat-clearing, is spitted blood, bleeding from five sense organs or subcutaneous tissue, and lung heat asthma is anxious, stomach energy hiccup, and gonorrhoea is small Just it is unfavorable, oedema, jaundice.

Corn stigma：With reducing blood lipid, blood glucose, diuresis, sharp liver and gall, corn stigma is also containing alkaloid, inositol, VC, VK, allantois Element etc..

Rhizoma Et Radix Notopterygii：Evacuate pathogenic wind is wet, sharp joint, and analgesic, master catches cold；It has a headache lossless；Mimeograph cold-dampness numbness；Geomantic omen edema；Sore Pyogenic infections.

Radix Angelicae Sinensis：The all cards of the deficiency of blood；Abdominal mass knot gathers；Uterine bleeding；Asthenia cold abdominalgia；Impotence numbness；Numbness；Intestines are dry and shit is difficult；Weight after dysentery characterized by blood in the stool； Ulcer sores；Injury from falling down.

Poria cocos：Difficult urination；Oedema turgor；Phlegm and retained fluid cough is inverse；Vomiting；Spleen eating less；Diarrhea；Palpitation and restlessness；Insomnia forgetfulness； Seminal emission gonorrhoea；Antitoxic heart-soothing and sedative, internal heat anticance；Mild in medicine property and, dampness removing is without hindering healthy tendency.

Radix cyathulae：Dispelling stasis of blood and stimulating the menstrual flow, easing joint movement, injury from falling down, arthralgia pain due to rheumatism, atrophy muscle.

Resveratrol：It finds resveratrol for the first time in nineteen thirty-nine, finds for the first time the 1970s in grape containing this Substance, later it is found that also containing this ingredient in the plants such as giant knotweed, peanut, mulberry fruit.Natural resveratrol is a kind of natural Active constituent, it can be in the form of two kinds of free state (cis-, trans-) and glucosides reference state (cis-, trans-) in plant (such as Chinese medicine Material giant knotweed) in be distributed and biosynthesis, and be respectively provided with antioxygenic effect, the bioactivity of wherein transisomer be better than it is cis-, A kind of important phytoalexin in grape, people conduct extensive research its natural resources, at least 21 sections, Resveratrol is found that in 72 kinds of plants of 31 categories, such as:The Vitis of Vitaceae, Ampelopsis, the Arachis of pulse family are determined Bright category, Sophora, liliaceous Veratrum, the eucalyptus category of Myrtaceae, Polygonum of polygonaceae etc., many plants containing resveratrol are normal The medicinal plant seen, such as Cassia, black false hellebore, giant knotweed, some are exactly food, such as:The content of resveratrol is most in grape, Grape Skin Height, up to 50~100mg/kg.

Corn stigma：With reducing blood lipid, blood glucose, diuresis, sharp liver and gall, corn stigma is also containing alkaloid, inositol, VC, VK, allantois Element etc.

Sargentodoxa cuneata：Toxin-Vanquishing disappears carbuncle, and acute and chronic appendicitis is controlled in promoting blood circulation and removing obstruction in channels, the effect of wind dispelling insecticide, and arthralgia pain due to rheumatism is red Dysentery, blood strangury, irregular menstruation, infantile malnutrition due to digestive disturbances or intestinalparasites, worm pain, injury from falling down.

Honeysuckle：Honeysuckle is cold in nature, sweet in flavor, enters lung, the heart, stomach, has effects that clearing heat and detoxicating, anti-inflammatory, qi-restoratives treats wind, Cure mainly disease under turgor, warm disease heating, the diseases such as heat toxin large carbuncle and tumour, for dizziness, thirst, sweating and boring, intestines Inflammation, bacillary dysentery, measles, pneumonia, encephalitis, epidemic meningitis, acute mastitis, septicemia, appendicitis, skin infection, ulcer boils, erysipelas, The illnesss such as parotitis, suppurative tonsillitis have certain curative effect.

The preparation method of above-mentioned plant extracts and probiotics compounding product, is as follows：

Step (2) after each strain fermentation, takes whole zymotic fluids to be centrifuged, concentrated, and takes the bacterium obtained after concentration Mud adds in protective agent by the 5%-15% of bacterium mud mass ratio, and protective agent can be powdered glucose, cane sugar powder, whey powder, lactose Any one in powder, serum powder, trehalose, sodium glutamate, glycerol yeast powder, maltodextrin or arbitrary several mixing, emulsification are equal It is freeze-dried or is spray-dried after even, make active microbe powder with high concentration or probiotics powder；

Step (4), by lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Switzerland obtained by step (2) Each viable bacteria powder of lactobacillus, lactobacillus bulgaricus, Lactobacillus paracasei carries out dispensing mixing, viable bacteria according to aforementioned mass percent Number control is 10⁶-10⁹CFU/ grams, it is prepared into complex solid powder；

Step (4) complex solid powder, step (5) extract and resveratrol are pressed aforementioned mass percent by step (6) Dispensing mixing is carried out, is performed by GMP working specifications, and operating environment humidity is less than 30%, is sufficiently mixed to obtain product.

The embodiment of the present invention and comparative example is given below.

Embodiment 1：

Probiotics provided in this embodiment compounds product with plant extracts, and compound probiotic is 35-40% in the product (mass percent), plant extracts are as follows for 60-65% (mass percent) preparation method：

Step (1) is stocked up, compound probiotic selection lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, rhamnose breast bar Bacterium, Lactobacillus helveticus, lactobacillus bulgaricus, Lactobacillus paracasei and saccharomyces cerevisiae, through in single strain routinely food Fermentation mixed culture obtains zymocyte liquid, and fermentation culture medium initial ph value is controlled in 4-8, and cultivation temperature is controlled at 30 DEG C extremely 50 DEG C, incubation time is subject to fermentation termination pH value to 3-6；

Step (2) after each strain fermentation, takes whole zymotic fluids to be centrifuged, concentrated, and takes the bacterium obtained after concentration Mud adds in protective agent by the 10% of bacterium mud mass ratio, and the protective agent of the present embodiment selects powdered glucose, is carried out after emulsifying uniformly cold Dry or spray drying is lyophilized, makes active microbe powder with high concentration；

Step (4) mixes the content dispensing by weight percentage of active microbe powder obtained by step (2)：Wherein, lactobacillus plantarum 9%th, Bifidobacterium Bifidum 8%, lactobacillus acidophilus 4%, Lactobacillus rhamnosus 4%, Lactobacillus helveticus 4%, lactobacillus bulgaricus 4%th, Lactobacillus paracasei 4% is prepared into complex solid powder；

Step (5), the preparation method of plant extracts specifically include each plant material stock, clean, drying, grinding is beaten Slurry, water extraction, extracting solution refined filtration are concentrated under reduced pressure, liquid extract, and extraction is stood still for crystals, and is filtered, coarse crystallization, dissolving, degreasing decoloring, Condensing crystallizing, recrystallization, coarse-grain, condensing crystallizing is dry, is sieved and obtains extract, and the withdrawal ratio of raw material and extract is 20： 1, each plant material is respectively rhizoma imperatae, corn stigma, Rhizoma Et Radix Notopterygii, Radix Angelicae Sinensis, Poria cocos, radix cyathulae, sargentodoxa cuneata and gold and silver in the present embodiment Flower, and extraction prepares extract respectively；

Step (5) each extract is carried out dispensing with resveratrol and mixes (rhizoma imperatae by step (6) by weight percentage 7%, corn stigma 8%, Rhizoma Et Radix Notopterygii 7%, Radix Angelicae Sinensis 7%, Poria cocos 7%, radix cyathulae 7%, sargentodoxa cuneata 5%, honeysuckle 5%, resveratrol 10%), mixture mixes again with step (4) complex solid powder, is performed by GMP working specifications, and operating environment humidity is small In 30%, it is sufficiently mixed to obtain the present embodiment product.

By detecting the present embodiment product viable bacteria content 10⁹CFU/ grams, water content is 6.0%.

Inventive article through several light, in, severe patient with gout is taken, significant effect, and treatment case is as follows：

1st, Beijing Mrs Wang, 40 years old, hobby meat, seafood, 5 years beer, moderate gout history, 410 μm of ol/ of blood uric acid value L, leg joint swelling and pain are unable to normal walking, and this product is used by crutches support.Take that product of the present invention, pain disappears after two weeks It loses, crutches normal walking is thrown away in naked joint detumescence, and uric acid, which is down to 295.4 μm of ol/L symptoms and disappears, restores normal.

2nd, Qinhuangdao Mr. Zhao, 51 years old, suffer from gout 27 years, 495 μm of ol/L both hands joint portions of blood uric acid value, leg, ankle Minister expires tophus, eats various gout medicines throughout the year, such as colchicin, Allopurinol, Febustat, even by perform the operation several times come Eradicating tophus, still effect is undesirable, and tophus is still grown again, and pain gets up to lie awake all night, along with long-term use is controlled It treats gout drug and grievous injury is caused to his liver kidney, disappeared using feeling of pain after this product 30 days, the detumescence of swelling position, gout Stone position is itched, and is generated heat, and reduces, gradually there is consciousness, continuing now with using.

3rd, Qinhuangdao Mr. Chu just suffered from gout, and 1 year uric acid height, ankle is in great pain, red and swollen, using 4 hours after this product Feeling of pain disappears, and subsides a swelling after 2 days.

Beneficial effects of the present invention are verified by the treatment case to embodiment and comparative example.Comparative example 1：

1 product of comparative example is prepared using the technical solution disclosed in CN201710671608.0, treatment case see the table below.

Comparative example 2：

2 product of comparative example is prepared using the technical solution disclosed in CN201310061824.5, treatment case see the table below.

Case list, blood uric acid value unit are treated with embodiment and comparative example product：μmol/L.

Since this invention takes above-mentioned technical proposals, compared with prior art, the present invention has high living to treating gout The advantages of property, high stability, function and effect fast, without side-effects, at low cost.Such a example has 100 many cases not going to live in the household of one's in-laws on getting married one by one It states.

Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, the equal variation or modification change completed under the technical spirit suggested by all present invention, should all belong to Cover the scope of the claims in the present invention.

Claims

1. a Plant Extracts compound product with probiotics, compound probiotic and plant extracts are re-dubbed, feature exists In compound probiotic matches composition by weight percent including following components：Lactobacillus plantarum 9%, Bifidobacterium Bifidum 8%, acidophilus Lactobacillus 4%, Lactobacillus rhamnosus 4%, Lactobacillus helveticus 4%, lactobacillus bulgaricus 4%, Lactobacillus paracasei 4%；It plants Object extract matches composition by weight percent including following components：Rhizoma imperatae 7%, corn stigma 8%, Rhizoma Et Radix Notopterygii 7%, Radix Angelicae Sinensis 7%, Poria cocos 7%, radix cyathulae 7%, resveratrol 10%, sargentodoxa cuneata 5%, honeysuckle 5%.

2. the preparation method of plant extracts according to claim 1 and probiotics compounding product, which is characterized in that specific Step is as follows：

Step (1), lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus helveticus, bulgarian milk Bacillus, Lactobacillus paracasei and saccharomyces cerevisiae stock, ferment through single strain and are mixed, and obtain zymocyte liquid, fermentation training The control of base initial ph value is supported in 4-8, cultivation temperature control at 30 DEG C to 50 DEG C, incubation time using fermentation termination pH value to 3-6 as It is accurate；

Step (2) after each strain fermentation, takes whole zymotic fluids to be centrifuged, concentrated, and takes the bacterium mud obtained after concentration, Protective agent is added in by the 5%-15% of bacterium mud mass ratio, is freeze-dried or is spray-dried after emulsification uniformly, make high concentration Active microbe powder or probiotics powder；

Step (3), in the lab carries out obtained each kind bacterium powder the inspection of technical indicator, and technical indicator includes aqueous According to the bacteria containing amount of recipe requirements in amount, miscellaneous bacteria infection and every gram of bacterium powder；

Step (4), by lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Switzerland's breast bar obtained by step (2) Each viable bacteria powder of bacterium, lactobacillus bulgaricus, Lactobacillus paracasei is according to aforementioned weight than carrying out dispensing mixing, viable count control At 106-109CFU/ grams, it is prepared into complex solid powder；

Step (5), the preparation method of plant extracts specifically include each plant material stock, clean, drying, grinding mashing, water Extraction, extracting solution refined filtration are concentrated under reduced pressure, liquid extract, and extraction is stood still for crystals, and is filtered, coarse crystallization, dissolving, degreasing decoloring, concentration Crystallization, recrystallization, coarse-grain, condensing crystallizing is dry, is sieved and obtains extract, and the withdrawal ratio of raw material and extract is 20：1.

Step (4) complex solid powder, step (5) extract and resveratrol are carried out dispensing by step (6) by aforementioned weight Mixing is performed by GMP working specifications, and operating environment humidity is less than 30%, is sufficiently mixed to obtain product.

3. the preparation method of plant extracts according to claim 2 and probiotics compounding product, which is characterized in that described Protective agent is powdered glucose, cane sugar powder, whey powder, lactose powder, serum powder, trehalose, sodium glutamate, glycerol yeast powder, malt Any one in dextrin or arbitrary several mixing.