CN108159291A - Plant extracts and probiotics compounding product and preparation method thereof - Google Patents
Plant extracts and probiotics compounding product and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a Plant Extracts and probiotics to compound product, compound probiotic and plant extracts is re-dubbed, compound probiotic matches composition by weight percent including following components：Lactobacillus plantarum 9%, Bifidobacterium Bifidum 8%, lactobacillus acidophilus 4%, Lactobacillus rhamnosus 4%, Lactobacillus helveticus 4%, lactobacillus bulgaricus 4%, Lactobacillus paracasei 4%；Plant extracts matches composition by weight percent including following components：Rhizoma imperatae 7%, corn stigma 8%, Rhizoma Et Radix Notopterygii 7%, Radix Angelicae Sinensis 7%, Poria cocos 7%, radix cyathulae 7%, resveratrol 10%, sargentodoxa cuneata 5%, honeysuckle 5%.The beneficial effects of the present invention are inventive article it is efficient, safe, harmless, good mouthfeel, can take for a long time, solve the problems, such as that function is single in existing product, effect is general, palliative, inconvenient to use.
Description
Technical field
It is specifically a kind of the present invention relates to liver protection, shield kidney and alleviation, the preparing technical field for treating gout product
Plant extracts and probiotics compounding product and preparation method thereof.
Background technology
It is higher with meat, aquatic product, soybean product content containing different purine in the food of our daily ingestions,
And vegetable and fruit class content is relatively fewer, in the synthesis and decomposable process of purine, there are many participations of enzyme, congenital due to enzyme
Sexual abnormality or certain not yet specific factor human body metabolisms get muddled, and the synthesis of uric acid is made to increase or discharge and is reduced, as a result
Hyperuricemia can be caused, this people is mostly related with heredity, and when serum Uric Acid Concentration is excessively high, uric acid is sunk in the form of sodium salt
Product causes the foreign matter inflammatory reaction of tissue, into the seed of trouble for causing gout in joint, soft tissue, cartilage and kidney.Nearly more than ten
Nian Lai, the higher people of blood uric acid is more and more, and the people of goat is also more and more, causes more high lithemias and gout patients
Reason is incessantly hereditary, scientific investigations showed that, the main reason is that, excessive diet causes kidney can't bear the heavy load, finally, kidney excretion
The function reduction of uric acid so that caused by uric acid is hoarded in human body.Purine nucleotides catabolic reaction basic process is nucleosides
Acid is ultimately breaks down into uric acid under the action of nucleotidase, external with urine ejection.Xanthine oxidase is important in catabolism
Enzyme.Purine nucleotides catabolism mainly carries out in liver, small intestine and kidney, purine metabolic disturbance：Uric acid excessively causes gout
Disease, uric acid content liter in patient's blood, thus, the people of hepatic and renal function exception, it is easier to gout must be gone up, eaten excessively when treating gout
Medicine not only without effect, can aggravate gout instead.
Probiotics refers to improve the active bacteria formulation and its metabolite of host health level and health status；Have in animal body
The bacterium or fungi of benefit mainly have：Lactic acid bacteria, Bifidobacterium, actinomyces, saccharomycete etc..The function of studying in the world at present is most strong
Big product be mainly above all kinds of microorganism groups into composite reactive probiotics；Prebiotic fungus strain is a kind of to the thin of animal health
Bacterium, they can direct catalysis polymer substance.
Enzyme is the large biological molecule for having biocatalytic Activity, i.e. biocatalyst, it can accelerate the speed of biochemical reaction
Degree, but do not change direction and the product of reaction.But unlike other abiotic catalyst, enzyme has the single-minded of height
Property, only catalysis specifically reacts or generates specific configuration.Thus single enzyme component is single, can only be catalyzed big point of a certain biology
Son is not particularly suited for diversified large biological molecule substrate, old friends in living nature and complex enzyme is conducted in-depth research,
It is developed and is increasingly paid close attention to.In the catalystic converter system of enzyme, reactant molecule is referred to as substrate, the catalysis that substrate passes through enzyme
Another molecule is converted into, almost all of cellular activity process is required for the participation organism of enzyme by cell composition, Mei Gexi
Since the presence of enzyme just shows various vital movements, internal metabolism could carry out born of the same parents.Enzyme is metabolic in human body
Catalyst, only enzyme exists, and items biochemical reaction could be carried out in human body.Human enzymes are more, more complete, and life is got over
Health.When also just terminating without organized enzyme, life in human body.Human diseases, it is most of with azymia or dyssynthesis
It is related.
For this purpose, we set about from the formula of product, efficient, safe, harmless, good mouthfeel, the plant that can be taken for a long time are developed
Object extract compounds product with probiotics.
Invention content
The defects of the purpose of the present invention is overcoming the prior art, provides a Plant Extracts and compounds product with probiotics,
The present invention therefrom preferably goes out that effect is best by the domestication culture to tens kinds of probiotics, and be most suitable for human body application 7 kinds are prebiotic
Bacterium carries out compatibility, screening, the experiment of more than 100 kind plants, final compounding product should achieve the effect that it is best, again using medicine
Homologous plant non-toxic to humans is eaten, so as to which the plant that obtains efficient, safe, harmless, good mouthfeel, can take for a long time carries
Object and probiotics is taken to compound product, solves that function in existing product is single, effect is general, palliative, inconvenient to use asks
Topic.
The invention is realized by the following technical scheme：
One Plant Extracts compound product with probiotics, and compound probiotic and plant extracts are re-dubbed, wherein, it is multiple
It closes probiotics and matches composition by weight percent including following components：Lactobacillus plantarum 9%, Bifidobacterium Bifidum 8%, lactobacillus acidophilus
4%th, Lactobacillus rhamnosus 4%, Lactobacillus helveticus 4%, lactobacillus bulgaricus 4%, Lactobacillus paracasei 4%；Plant extract
Object matches composition by weight percent including following components：Rhizoma imperatae 7%, corn stigma 8%, Rhizoma Et Radix Notopterygii 7%, Radix Angelicae Sinensis 7%, Poria cocos
7%, radix cyathulae 7%, resveratrol 10%, sargentodoxa cuneata 5%, honeysuckle 5%.
The invention solves another technical problem be to provide the system of above-mentioned plant extracts and probiotics compounding product
Preparation Method is as follows：
Step (1), lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus helveticus, Bao Jiali
Sub- lactobacillus, Lactobacillus paracasei and saccharomyces cerevisiae stock, ferment through single strain and are mixed, and obtain zymocyte liquid, fermentation
With the control of culture medium initial ph value in 4-8, cultivation temperature is controlled at 30 DEG C to 50 DEG C, and incubation time is with fermentation termination pH value to 3-
Subject to 6；
Step (2) after each strain fermentation, takes whole zymotic fluids to be centrifuged, concentrated, and takes the bacterium obtained after concentration
Mud adds in protective agent by the 5%-15% of bacterium mud mass ratio, is freeze-dried or is spray-dried after emulsification uniformly, made highly concentrated
Spend active microbe powder or probiotics powder；
Step (3), in the lab carries out obtained each kind bacterium powder the inspection of technical indicator, and technical indicator includes
According to the bacteria containing amount of recipe requirements in water content, miscellaneous bacteria infection and every gram of bacterium powder；
Step (4), by lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Switzerland obtained by step (2)
Each viable bacteria powder of lactobacillus, lactobacillus bulgaricus, Lactobacillus paracasei is according to aforementioned weight than carrying out dispensing mixing, viable count
Control is 106-109CFU/ grams, it is prepared into complex solid powder；
Step (5), the preparation method of plant extracts specifically include each plant material stock, clean, drying, grinding is beaten
Slurry, water extraction, extracting solution refined filtration are concentrated under reduced pressure, liquid extract, and extraction is stood still for crystals, and is filtered, coarse crystallization, dissolving, degreasing decoloring,
Condensing crystallizing, recrystallization, coarse-grain, condensing crystallizing is dry, is sieved and obtains extract, and the withdrawal ratio of raw material and extract is 20：
1。
Step (6) is carried out step (4) complex solid powder, step (5) extract and resveratrol by aforementioned weight
Dispensing mixes, and is performed by GMP working specifications, and operating environment humidity is less than 30%, is sufficiently mixed to obtain product.
Preferably, the protective agent is powdered glucose, cane sugar powder, whey powder, lactose powder, serum powder, trehalose, glutamic acid
Any one in sodium, glycerol yeast powder, maltodextrin or arbitrary several mixing.
The beneficial effects of the present invention are：
Inventive article enters purine human body with bidirectional modulation and decomposition, and probiotics is by institute's purine-containing in food
It is decomposed when passing through intestinal absorption, enhancing body quickly removes most purine, and by probiotics in enteron aisle
The polypeptide for generating and being largely beneficial to health is colonized, blood fat is high because gout patients largely have, high blood pressure, the spies such as blood glucose height
Point, the present invention, which also takes into account, adjusts blood pressure, blood glucose, blood fat and other effects.In addition produced by the present invention in the plant extracts of compatibility
Distinctive plant alkaloid, the product of purine, uric acid for the symptom of first gout, can such as suffer from effectively comprehensive and blood
Locate pain, redness, have analgesic antiphlogistic, detumescence effect, for severe gout patient articular position, ligament tissue accumulates a large amount of gouts
The patient of stone also has the effect for dissolving tophus in addition to above-mentioned function.
Lactobacillus helveticus in the present invention is capable of a variety of biologically active peptides beneficial to human body of lactoalbumin hydrolysate generation, tool
There is the angiotoninase that inhibition causes hypertension, active effect blood pressure reduction effect has improvement sleep and increases blood calcium concentration
Effect；Lactobacillus plantarum has certain immunoregulation effect, there is inhibiting effect to pathogenic bacteria, reduce serum cholesterol content and
Prevention cardiovascular and cerebrovascular is cured the disease, and intestinal flora balance is maintained to promote absorption of nutrient ingredients, alleviates lactose intolerance, inhibits tumour thin
The formation of born of the same parents；Lactobacillus bulgaricus can adjust intestinal flora balance, improve constipation, reduce cholesterol, improve liver function, control
Vivotoxin, the growth for inhibiting spoilage organisms in enteron aisle, manufacture nutriment, the development for stimulating healthy cell.
Specific embodiment
Illustrate this patent with example below, be then tested for the property.It should be noted that the embodiments described below
It is exemplary, is only used for explaining the present invention, and it is not intended that limitation to this patent.
One Plant Extracts compound product with probiotics, and compound probiotic and plant extracts are re-dubbed, wherein, it is multiple
It closes probiotics and includes following components：Lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus helveticus,
Lactobacillus bulgaricus, Lactobacillus paracasei；Plant extracts matches composition by weight percent including following components：Cogongrass
Root, corn stigma, Rhizoma Et Radix Notopterygii, Radix Angelicae Sinensis, Poria cocos, radix cyathulae, sargentodoxa cuneata, honeysuckle, resveratrol.
Bifidobacterium：Treatment chronic diarrhea, which improves intestinal environment, can simultaneously treat because largely using antibiotic；
Lactobacillus paracasei：It can promote the balance of body microorganism species and enzyme and the immunologic mechanism of stimulation specificity
It can play prevention disease, development is promoted to build up health, slow down aging and extend the effect in service life；
Lactobacillus acidophilus：Inhibit the increment of enteron aisle undesirable microorganism has antagonism to pathogenic microorganisms；
Lactobacillus rhamnosus：Balance improves function of intestinal canal, enhances human autoimmune's ability, promotes Bifidobacterium and acidophilus
The growth of lactobacillus, prevention help to treat diarrhea, preventing respiratory tract infection, exclude toxin, is pre- anti-caries, pre- hypo-allergenic.
Lactobacillus helveticus：It is capable of a variety of biologically active peptides beneficial to human body of lactoalbumin hydrolysate generation, has and inhibit to draw
The angiotoninase of hypertension is played, active effect blood pressure reduction effect plays the role of improving sleep and increases blood calcium concentration；
Lactobacillus plantarum：There is certain immunoregulation effect, there is inhibiting effect to pathogenic bacteria, reduce serum cholesterol content
It cures the disease with prevention cardiovascular and cerebrovascular, intestinal flora balance is maintained to promote absorption of nutrient ingredients, alleviate lactose intolerance, inhibit tumour
The formation of cell；Inhibit the growth of spoilage organisms in enteron aisle, manufacture nutriment, the development for stimulating healthy cell.
Lactobacillus bulgaricus：Intestinal flora balance is adjusted, improves constipation, reduces cholesterol, improve liver function, inhibit body
Endotoxin is grown.
Rhizoma imperatae：Pyreticosis polydipsia is controlled in cool blood, hemostasis, heat-clearing, is spitted blood, bleeding from five sense organs or subcutaneous tissue, and lung heat asthma is anxious, stomach energy hiccup, and gonorrhoea is small
Just it is unfavorable, oedema, jaundice.
Corn stigma：With reducing blood lipid, blood glucose, diuresis, sharp liver and gall, corn stigma is also containing alkaloid, inositol, VC, VK, allantois
Element etc..
Rhizoma Et Radix Notopterygii：Evacuate pathogenic wind is wet, sharp joint, and analgesic, master catches cold；It has a headache lossless；Mimeograph cold-dampness numbness；Geomantic omen edema；Sore
Pyogenic infections.
Radix Angelicae Sinensis：The all cards of the deficiency of blood；Abdominal mass knot gathers；Uterine bleeding；Asthenia cold abdominalgia；Impotence numbness；Numbness；Intestines are dry and shit is difficult；Weight after dysentery characterized by blood in the stool；
Ulcer sores；Injury from falling down.
Poria cocos：Difficult urination；Oedema turgor；Phlegm and retained fluid cough is inverse；Vomiting；Spleen eating less；Diarrhea；Palpitation and restlessness；Insomnia forgetfulness；
Seminal emission gonorrhoea；Antitoxic heart-soothing and sedative, internal heat anticance；Mild in medicine property and, dampness removing is without hindering healthy tendency.
Radix cyathulae：Dispelling stasis of blood and stimulating the menstrual flow, easing joint movement, injury from falling down, arthralgia pain due to rheumatism, atrophy muscle.
Resveratrol：It finds resveratrol for the first time in nineteen thirty-nine, finds for the first time the 1970s in grape containing this
Substance, later it is found that also containing this ingredient in the plants such as giant knotweed, peanut, mulberry fruit.Natural resveratrol is a kind of natural
Active constituent, it can be in the form of two kinds of free state (cis-, trans-) and glucosides reference state (cis-, trans-) in plant (such as Chinese medicine
Material giant knotweed) in be distributed and biosynthesis, and be respectively provided with antioxygenic effect, the bioactivity of wherein transisomer be better than it is cis-,
A kind of important phytoalexin in grape, people conduct extensive research its natural resources, at least 21 sections,
Resveratrol is found that in 72 kinds of plants of 31 categories, such as:The Vitis of Vitaceae, Ampelopsis, the Arachis of pulse family are determined
Bright category, Sophora, liliaceous Veratrum, the eucalyptus category of Myrtaceae, Polygonum of polygonaceae etc., many plants containing resveratrol are normal
The medicinal plant seen, such as Cassia, black false hellebore, giant knotweed, some are exactly food, such as:The content of resveratrol is most in grape, Grape Skin
Height, up to 50~100mg/kg.
Corn stigma：With reducing blood lipid, blood glucose, diuresis, sharp liver and gall, corn stigma is also containing alkaloid, inositol, VC, VK, allantois
Element etc.
Sargentodoxa cuneata：Toxin-Vanquishing disappears carbuncle, and acute and chronic appendicitis is controlled in promoting blood circulation and removing obstruction in channels, the effect of wind dispelling insecticide, and arthralgia pain due to rheumatism is red
Dysentery, blood strangury, irregular menstruation, infantile malnutrition due to digestive disturbances or intestinalparasites, worm pain, injury from falling down.
Honeysuckle：Honeysuckle is cold in nature, sweet in flavor, enters lung, the heart, stomach, has effects that clearing heat and detoxicating, anti-inflammatory, qi-restoratives treats wind,
Cure mainly disease under turgor, warm disease heating, the diseases such as heat toxin large carbuncle and tumour, for dizziness, thirst, sweating and boring, intestines
Inflammation, bacillary dysentery, measles, pneumonia, encephalitis, epidemic meningitis, acute mastitis, septicemia, appendicitis, skin infection, ulcer boils, erysipelas,
The illnesss such as parotitis, suppurative tonsillitis have certain curative effect.
The preparation method of above-mentioned plant extracts and probiotics compounding product, is as follows：
Step (1), lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus helveticus, Bao Jiali
Sub- lactobacillus, Lactobacillus paracasei and saccharomyces cerevisiae stock, ferment through single strain and are mixed, and obtain zymocyte liquid, fermentation
With the control of culture medium initial ph value in 4-8, cultivation temperature is controlled at 30 DEG C to 50 DEG C, and incubation time is with fermentation termination pH value to 3-
Subject to 6；
Step (2) after each strain fermentation, takes whole zymotic fluids to be centrifuged, concentrated, and takes the bacterium obtained after concentration
Mud adds in protective agent by the 5%-15% of bacterium mud mass ratio, and protective agent can be powdered glucose, cane sugar powder, whey powder, lactose
Any one in powder, serum powder, trehalose, sodium glutamate, glycerol yeast powder, maltodextrin or arbitrary several mixing, emulsification are equal
It is freeze-dried or is spray-dried after even, make active microbe powder with high concentration or probiotics powder；
Step (3), in the lab carries out obtained each kind bacterium powder the inspection of technical indicator, and technical indicator includes
According to the bacteria containing amount of recipe requirements in water content, miscellaneous bacteria infection and every gram of bacterium powder；
Step (4), by lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Switzerland obtained by step (2)
Each viable bacteria powder of lactobacillus, lactobacillus bulgaricus, Lactobacillus paracasei carries out dispensing mixing, viable bacteria according to aforementioned mass percent
Number control is 106-109CFU/ grams, it is prepared into complex solid powder；
Step (5), the preparation method of plant extracts specifically include each plant material stock, clean, drying, grinding is beaten
Slurry, water extraction, extracting solution refined filtration are concentrated under reduced pressure, liquid extract, and extraction is stood still for crystals, and is filtered, coarse crystallization, dissolving, degreasing decoloring,
Condensing crystallizing, recrystallization, coarse-grain, condensing crystallizing is dry, is sieved and obtains extract, and the withdrawal ratio of raw material and extract is 20：
1。
Step (4) complex solid powder, step (5) extract and resveratrol are pressed aforementioned mass percent by step (6)
Dispensing mixing is carried out, is performed by GMP working specifications, and operating environment humidity is less than 30%, is sufficiently mixed to obtain product.
The embodiment of the present invention and comparative example is given below.
Embodiment 1：
Probiotics provided in this embodiment compounds product with plant extracts, and compound probiotic is 35-40% in the product
(mass percent), plant extracts are as follows for 60-65% (mass percent) preparation method：
Step (1) is stocked up, compound probiotic selection lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, rhamnose breast bar
Bacterium, Lactobacillus helveticus, lactobacillus bulgaricus, Lactobacillus paracasei and saccharomyces cerevisiae, through in single strain routinely food
Fermentation mixed culture obtains zymocyte liquid, and fermentation culture medium initial ph value is controlled in 4-8, and cultivation temperature is controlled at 30 DEG C extremely
50 DEG C, incubation time is subject to fermentation termination pH value to 3-6；
Step (2) after each strain fermentation, takes whole zymotic fluids to be centrifuged, concentrated, and takes the bacterium obtained after concentration
Mud adds in protective agent by the 10% of bacterium mud mass ratio, and the protective agent of the present embodiment selects powdered glucose, is carried out after emulsifying uniformly cold
Dry or spray drying is lyophilized, makes active microbe powder with high concentration；
Step (3), in the lab carries out obtained each kind bacterium powder the inspection of technical indicator, and technical indicator includes
According to the bacteria containing amount of recipe requirements in water content, miscellaneous bacteria infection and every gram of bacterium powder；
Step (4) mixes the content dispensing by weight percentage of active microbe powder obtained by step (2)：Wherein, lactobacillus plantarum
9%th, Bifidobacterium Bifidum 8%, lactobacillus acidophilus 4%, Lactobacillus rhamnosus 4%, Lactobacillus helveticus 4%, lactobacillus bulgaricus
4%th, Lactobacillus paracasei 4% is prepared into complex solid powder；
Step (5), the preparation method of plant extracts specifically include each plant material stock, clean, drying, grinding is beaten
Slurry, water extraction, extracting solution refined filtration are concentrated under reduced pressure, liquid extract, and extraction is stood still for crystals, and is filtered, coarse crystallization, dissolving, degreasing decoloring,
Condensing crystallizing, recrystallization, coarse-grain, condensing crystallizing is dry, is sieved and obtains extract, and the withdrawal ratio of raw material and extract is 20：
1, each plant material is respectively rhizoma imperatae, corn stigma, Rhizoma Et Radix Notopterygii, Radix Angelicae Sinensis, Poria cocos, radix cyathulae, sargentodoxa cuneata and gold and silver in the present embodiment
Flower, and extraction prepares extract respectively；
Step (5) each extract is carried out dispensing with resveratrol and mixes (rhizoma imperatae by step (6) by weight percentage
7%, corn stigma 8%, Rhizoma Et Radix Notopterygii 7%, Radix Angelicae Sinensis 7%, Poria cocos 7%, radix cyathulae 7%, sargentodoxa cuneata 5%, honeysuckle 5%, resveratrol
10%), mixture mixes again with step (4) complex solid powder, is performed by GMP working specifications, and operating environment humidity is small
In 30%, it is sufficiently mixed to obtain the present embodiment product.
By detecting the present embodiment product viable bacteria content 109CFU/ grams, water content is 6.0%.
Inventive article through several light, in, severe patient with gout is taken, significant effect, and treatment case is as follows：
1st, Beijing Mrs Wang, 40 years old, hobby meat, seafood, 5 years beer, moderate gout history, 410 μm of ol/ of blood uric acid value
L, leg joint swelling and pain are unable to normal walking, and this product is used by crutches support.Take that product of the present invention, pain disappears after two weeks
It loses, crutches normal walking is thrown away in naked joint detumescence, and uric acid, which is down to 295.4 μm of ol/L symptoms and disappears, restores normal.
2nd, Qinhuangdao Mr. Zhao, 51 years old, suffer from gout 27 years, 495 μm of ol/L both hands joint portions of blood uric acid value, leg, ankle
Minister expires tophus, eats various gout medicines throughout the year, such as colchicin, Allopurinol, Febustat, even by perform the operation several times come
Eradicating tophus, still effect is undesirable, and tophus is still grown again, and pain gets up to lie awake all night, along with long-term use is controlled
It treats gout drug and grievous injury is caused to his liver kidney, disappeared using feeling of pain after this product 30 days, the detumescence of swelling position, gout
Stone position is itched, and is generated heat, and reduces, gradually there is consciousness, continuing now with using.
3rd, Qinhuangdao Mr. Chu just suffered from gout, and 1 year uric acid height, ankle is in great pain, red and swollen, using 4 hours after this product
Feeling of pain disappears, and subsides a swelling after 2 days.
Beneficial effects of the present invention are verified by the treatment case to embodiment and comparative example.Comparative example 1：
1 product of comparative example is prepared using the technical solution disclosed in CN201710671608.0, treatment case see the table below.
Comparative example 2：
2 product of comparative example is prepared using the technical solution disclosed in CN201310061824.5, treatment case see the table below.
Case list, blood uric acid value unit are treated with embodiment and comparative example product：μmol/L.
Since this invention takes above-mentioned technical proposals, compared with prior art, the present invention has high living to treating gout
The advantages of property, high stability, function and effect fast, without side-effects, at low cost.Such a example has 100 many cases not going to live in the household of one's in-laws on getting married one by one
It states.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal variation or modification change completed under the technical spirit suggested by all present invention, should all belong to
Cover the scope of the claims in the present invention.
Claims (3)
1. a Plant Extracts compound product with probiotics, compound probiotic and plant extracts are re-dubbed, feature exists
In compound probiotic matches composition by weight percent including following components：Lactobacillus plantarum 9%, Bifidobacterium Bifidum 8%, acidophilus
Lactobacillus 4%, Lactobacillus rhamnosus 4%, Lactobacillus helveticus 4%, lactobacillus bulgaricus 4%, Lactobacillus paracasei 4%；It plants
Object extract matches composition by weight percent including following components：Rhizoma imperatae 7%, corn stigma 8%, Rhizoma Et Radix Notopterygii 7%, Radix Angelicae Sinensis 7%,
Poria cocos 7%, radix cyathulae 7%, resveratrol 10%, sargentodoxa cuneata 5%, honeysuckle 5%.
2. the preparation method of plant extracts according to claim 1 and probiotics compounding product, which is characterized in that specific
Step is as follows：
Step (1), lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus helveticus, bulgarian milk
Bacillus, Lactobacillus paracasei and saccharomyces cerevisiae stock, ferment through single strain and are mixed, and obtain zymocyte liquid, fermentation training
The control of base initial ph value is supported in 4-8, cultivation temperature control at 30 DEG C to 50 DEG C, incubation time using fermentation termination pH value to 3-6 as
It is accurate；
Step (2) after each strain fermentation, takes whole zymotic fluids to be centrifuged, concentrated, and takes the bacterium mud obtained after concentration,
Protective agent is added in by the 5%-15% of bacterium mud mass ratio, is freeze-dried or is spray-dried after emulsification uniformly, make high concentration
Active microbe powder or probiotics powder；
Step (3), in the lab carries out obtained each kind bacterium powder the inspection of technical indicator, and technical indicator includes aqueous
According to the bacteria containing amount of recipe requirements in amount, miscellaneous bacteria infection and every gram of bacterium powder；
Step (4), by lactobacillus plantarum, Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus rhamnosus, Switzerland's breast bar obtained by step (2)
Each viable bacteria powder of bacterium, lactobacillus bulgaricus, Lactobacillus paracasei is according to aforementioned weight than carrying out dispensing mixing, viable count control
At 106-109CFU/ grams, it is prepared into complex solid powder；
Step (5), the preparation method of plant extracts specifically include each plant material stock, clean, drying, grinding mashing, water
Extraction, extracting solution refined filtration are concentrated under reduced pressure, liquid extract, and extraction is stood still for crystals, and is filtered, coarse crystallization, dissolving, degreasing decoloring, concentration
Crystallization, recrystallization, coarse-grain, condensing crystallizing is dry, is sieved and obtains extract, and the withdrawal ratio of raw material and extract is 20：1.
Step (4) complex solid powder, step (5) extract and resveratrol are carried out dispensing by step (6) by aforementioned weight
Mixing is performed by GMP working specifications, and operating environment humidity is less than 30%, is sufficiently mixed to obtain product.
3. the preparation method of plant extracts according to claim 2 and probiotics compounding product, which is characterized in that described
Protective agent is powdered glucose, cane sugar powder, whey powder, lactose powder, serum powder, trehalose, sodium glutamate, glycerol yeast powder, malt
Any one in dextrin or arbitrary several mixing.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108936678A (en) * | 2018-08-08 | 2018-12-07 | 深圳极醇健康产业股份有限公司 | A kind of probiotics capsule and preparation method thereof |
| CN110354207A (en) * | 2019-08-24 | 2019-10-22 | 河南中微生物科技研究院(有限合伙) | A kind of fermented tcm oral solution and preparation method thereof improving gastrointestinal health of animals |
| CN113925169A (en) * | 2021-11-23 | 2022-01-14 | 天津小薇生物科技有限公司 | Gout relieving probiotic composition, and preparation method and application thereof |
| CN116616447A (en) * | 2023-05-25 | 2023-08-22 | 杭州知零生物科技有限公司 | Metazoan microcapsule and preparation method thereof |
| NL2035839B1 (en) * | 2022-11-23 | 2024-05-30 | Guizhou Zhende Biotechnology Co Ltd | Medicinal and edible homologous enzyme with uric acid lowering effect and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160124630A (en) * | 2015-04-20 | 2016-10-28 | 이동한 | The method of manufacturing the functional improvement in ventilation efficiency using theHydroponic cultivation and foliar sibibeop |
| CN107259578A (en) * | 2017-08-08 | 2017-10-20 | 韩大春 | A kind of probiotic composition and preparation method thereof |
-
2017
- 2017-12-07 CN CN201711287884.3A patent/CN108159291A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160124630A (en) * | 2015-04-20 | 2016-10-28 | 이동한 | The method of manufacturing the functional improvement in ventilation efficiency using theHydroponic cultivation and foliar sibibeop |
| CN107259578A (en) * | 2017-08-08 | 2017-10-20 | 韩大春 | A kind of probiotic composition and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 田润安等: "中医辨证治疗痛风性关节炎60例", 《陕西中医》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108936678A (en) * | 2018-08-08 | 2018-12-07 | 深圳极醇健康产业股份有限公司 | A kind of probiotics capsule and preparation method thereof |
| CN110354207A (en) * | 2019-08-24 | 2019-10-22 | 河南中微生物科技研究院(有限合伙) | A kind of fermented tcm oral solution and preparation method thereof improving gastrointestinal health of animals |
| CN110354207B (en) * | 2019-08-24 | 2021-06-04 | 河南中微生物科技研究院(有限合伙) | Fermented traditional Chinese medicine oral liquid for improving gastrointestinal health of animals and preparation method thereof |
| CN113925169A (en) * | 2021-11-23 | 2022-01-14 | 天津小薇生物科技有限公司 | Gout relieving probiotic composition, and preparation method and application thereof |
| NL2035839B1 (en) * | 2022-11-23 | 2024-05-30 | Guizhou Zhende Biotechnology Co Ltd | Medicinal and edible homologous enzyme with uric acid lowering effect and preparation method thereof |
| CN116616447A (en) * | 2023-05-25 | 2023-08-22 | 杭州知零生物科技有限公司 | Metazoan microcapsule and preparation method thereof |
| CN116616447B (en) * | 2023-05-25 | 2024-09-17 | 杭州知零生物科技有限公司 | Metazoan microcapsule and preparation method thereof |
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Application publication date: 20180615 |